Trends in Analytical Chemistry最新文献_第9页

Probing interfacial electrochemical processes in metal biodegradation via in operando pH microscopy: A review with perspectives 利用酸碱度显微镜探测金属生物降解中的界面电化学过程：综述与展望

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2025-12-24 DOI: 10.1016/j.trac.2025.118619

Cheng Wang , Qiangsheng Dong , Yi Shao , Yulong Wu , Yanbin Zhao , Ming Yin , Di Mei , Chenglin Chu , Feng Xue , Jing Bai , Mikhail L. Zheludkevich , Sviatlana V. Lamaka

{"title":"Probing interfacial electrochemical processes in metal biodegradation via in operando pH microscopy: A review with perspectives","authors":"Cheng Wang , Qiangsheng Dong , Yi Shao , Yulong Wu , Yanbin Zhao , Ming Yin , Di Mei , Chenglin Chu , Feng Xue , Jing Bai , Mikhail L. Zheludkevich , Sviatlana V. Lamaka","doi":"10.1016/j.trac.2025.118619","DOIUrl":"10.1016/j.trac.2025.118619","url":null,"abstract":"<div><div>Metal biodegradation is primarily an electrochemical process, involving metal anodic dissolution and cathodic reduction of water or oxygen. The hydrolysis of metal cations produces H<sup>+</sup> ions, while the cathodic reactions generate OH<sup>−</sup> ions, inducing the changes in local pH. These pH variations are also influenced by the precipitation of chemical species on a metal surface. To monitor the local pH evolution, pH microelectrodes are placed in an electrolyte solution above the active surface (10–100 μm). This technique plays a crucial role in discerning the chemical nature of degradation products and getting insights on the kinetics of coupled electrochemical-chemical processes, especially in complex media during the degradation of biodegradable metals. This review provides an introduction to pH microscopy, presents several application examples in biodegradation-related studies, and discusses the simultaneous utilization of pH microscopy with other local techniques.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118619"},"PeriodicalIF":12.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145882557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent advancements in molecular diagnostics with nanomaterials 纳米材料在分子诊断中的最新进展

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2025-12-23 DOI: 10.1016/j.trac.2025.118605

Jing Li , Jiao Hu , Dai-Wen Pang , Cui Liu

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Nucleic acid-assisted metabolic glycan labeling: A promising strategy for enhanced glycoconjugate imaging in cells and in vivo 核酸辅助代谢聚糖标记：一种在细胞和体内增强糖结合成像的有前途的策略

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2026-01-21 DOI: 10.1016/j.trac.2026.118692

Sirui Liu , Mengyu Huang , Jin Li , Shulin Wang , Wei Lv , Wanshuang Cao , Bingzhi Li , Xing Zhang

{"title":"Nucleic acid-assisted metabolic glycan labeling: A promising strategy for enhanced glycoconjugate imaging in cells and in vivo","authors":"Sirui Liu , Mengyu Huang , Jin Li , Shulin Wang , Wei Lv , Wanshuang Cao , Bingzhi Li , Xing Zhang","doi":"10.1016/j.trac.2026.118692","DOIUrl":"10.1016/j.trac.2026.118692","url":null,"abstract":"<div><div>Glycoconjugates are central to cellular recognition, signaling, and adhesion, yet their visualization in living systems remains difficult because of low abundance, structural diversity, and the limited specificity of traditional probes. Metabolic glycan labeling provides a means to introduce bioorthogonal groups into cellular glycans, allowing selective attachment of imaging probes. Recent studies have combined this approach with functional nucleic acid technologies such as aptamers, CRISPR/Cas systems, DNAzymes, and isothermal amplification. These combinations improve signal strength, increase selectivity, and support multiplex or multimodal readouts. The programmability and amplification capability of nucleic acids help address long-standing challenges in sensitivity and molecular recognition. This review summarizes recent progress in nucleic acid-assisted metabolic glycan labeling for visualizing glycoconjugates in live cells and <em>in vivo</em> models, outlines representative analytical formats, and highlights remaining challenges. Future opportunities are discussed with attention to expanding the analytical toolkit for glycobiology and molecular imaging.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118692"},"PeriodicalIF":12.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Mapping metabolism using stable isotope tracers in combination with mass spectrometry imaging 利用稳定同位素示踪剂结合质谱成像绘制代谢图谱

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2026-01-16 DOI: 10.1016/j.trac.2026.118679

Sarantos Kostidis , Hannah Mörk , Ton J. Rabelink , Martin Giera , Nina Ogrinc

{"title":"Mapping metabolism using stable isotope tracers in combination with mass spectrometry imaging","authors":"Sarantos Kostidis , Hannah Mörk , Ton J. Rabelink , Martin Giera , Nina Ogrinc","doi":"10.1016/j.trac.2026.118679","DOIUrl":"10.1016/j.trac.2026.118679","url":null,"abstract":"<div><div>Stable isotope tracing combined with mass spectrometry imaging (MSI) offers a powerful framework for spatially and temporally resolved analysis of metabolic activity, enabling visualization of labeled metabolites at cellular and subcellular resolution. This approach provides insights into the spatial organization and dynamics of metabolic pathways such as glycolysis, the tricarboxylic acid cycle, and amino acid metabolism under both physiological and pathological conditions. However, its implementation remains technically challenging due to spectral complexity, limited spatial quantification methods, and the need for meticulous sample preparation and data processing. This review defines key considerations for integrating isotope tracers with MSI, including tracer selection, administration strategies across biological model systems, and current MSI platform capabilities. It also covers interpretation of isotopic labeling patterns and emerging experimental designs for mapping metabolic activity. By consolidating recent advances and identifying ongoing challenges, this review outlines the evolving role of MSI-based isotope tracing in spatial metabolomics.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118679"},"PeriodicalIF":12.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Advancements of multiple template-based molecularly imprinted polymers (mt-MIPs) in deep eutectic solvents (DESs): Towards greener imprinting approaches 深度共晶溶剂（DESs）中多模板基分子印迹聚合物（mt-MIPs）的研究进展：迈向绿色印迹方法

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2026-01-14 DOI: 10.1016/j.trac.2026.118673

Shuai Zhang , Kavirajaa Pandian Sambasevam , Faizah Muhammad Yunus , Waleed Alahmad , Nor Kartini Binti Abu Bakar , Muggundha Raoov

{"title":"Advancements of multiple template-based molecularly imprinted polymers (mt-MIPs) in deep eutectic solvents (DESs): Towards greener imprinting approaches","authors":"Shuai Zhang , Kavirajaa Pandian Sambasevam , Faizah Muhammad Yunus , Waleed Alahmad , Nor Kartini Binti Abu Bakar , Muggundha Raoov","doi":"10.1016/j.trac.2026.118673","DOIUrl":"10.1016/j.trac.2026.118673","url":null,"abstract":"<div><div>This review is the first to provide a systematic integration of deep eutectic solvents (DESs) into multi-template molecular imprinting, establishing DESs-based multi-template molecularly imprinted polymers (DESs-mt-MIPs) as an emerging class of green affinity materials. The review systematically covers the fundamental principles of molecular imprinting, emphasizing the advantages and mechanisms of DESs-based multiple-template imprinting over traditional MIPs approaches. This review also investigates the pivotal functions of DESs as both functional monomers and porogens in the fabrication of mt-MIPs, demonstrating their ability to enhance the polymers' selectivity, adsorption capacity, and environmental friendliness. Furthermore, the review presents various innovative synthesis techniques, characterizes the structural and functional properties of DESs-mt-MIPs, and discusses their applications in environmental pollutant removal, biomedical fields, and food safety. Despite the promising potential, current challenges, including the type of DESs that can be used in MIPs synthesis, the combination of multiple templates, stability, reproducibility, and large-scale production, are highlighted, and future research directions are proposed to address these issues and explore mt-MIPs in DESs further.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118673"},"PeriodicalIF":12.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent advances in application of artificial intelligence algorithms in terahertz sensing techniques 人工智能算法在太赫兹传感技术中的应用进展

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2026-01-01 DOI: 10.1016/j.trac.2025.118645

Jianyuan Qin, Xing Wu, Fang Wang, Binggang Xiao, Yong Du

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Technologies and opportunities in cell-free RNA analysis for clinical diagnostics 临床诊断中无细胞RNA分析的技术和机遇

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2025-12-31 DOI: 10.1016/j.trac.2025.118640

Linyi Deng , Da Han

{"title":"Technologies and opportunities in cell-free RNA analysis for clinical diagnostics","authors":"Linyi Deng , Da Han","doi":"10.1016/j.trac.2025.118640","DOIUrl":"10.1016/j.trac.2025.118640","url":null,"abstract":"<div><div>Cell-free RNA (cfRNA) circulating in biofluids comprises RNA molecules originating from diverse tissues and cell types, providing a dynamic and real-time snapshot of the body that can be harnessed for early disease detection, differential diagnosis, and longitudinal monitoring. Nevertheless, clinical translation of cfRNA analysis is hindered by pronounced variability arising from diverse pre-analytical conditions, as well as the immaturity and limited standardization of subsequent analytical pipelines. Recent advances in sequencing technologies, together with optimized library preparation protocols and computational frameworks, have substantially improved the sensitivity, reproducibility, and interpretability of cfRNA profiling, thereby paving the way for its broader diagnostic and biomedical applications. In this Review, we highlight the critical impact of pre-analytical workflows on cfRNA studies, provide a summary of current technologies from low-plex assays to high-throughput sequencing, explore the promising potential of cfRNA in clinical applications, and conclude with a discussion of current challenges and future directions.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118640"},"PeriodicalIF":12.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent progress in microfluidic cell manipulation techniques and their applications in single-cell mass spectrometry 微流控细胞操作技术及其在单细胞质谱分析中的应用进展

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Trends in Analytical Chemistry Pub Date : 2026-03-01 Epub Date: 2025-12-25 DOI: 10.1016/j.trac.2025.118630

Siyuan Tan , Huiping Qiao , Xuping Shentu , Zehu Xie , Yu Wang , Qionglin Liang , Xiaoyun Gong , Xinhua Dai

{"title":"Recent progress in microfluidic cell manipulation techniques and their applications in single-cell mass spectrometry","authors":"Siyuan Tan , Huiping Qiao , Xuping Shentu , Zehu Xie , Yu Wang , Qionglin Liang , Xiaoyun Gong , Xinhua Dai","doi":"10.1016/j.trac.2025.118630","DOIUrl":"10.1016/j.trac.2025.118630","url":null,"abstract":"<div><div>With the accelerated advancement of biotechnology, microfluidic cell manipulation has emerged as a focal research area. Microfluidics leverages microscale channels to achieve precise control of fluids with high throughput, cost-effectiveness, and miniaturization. It makes use of the unique properties of fluids for single-cell manipulation and analysis with unprecedented accuracy and efficiency. With the advantages of high sensitivity, wide molecular coverage and excellent structural identification, mass spectrometry (MS) stands as a powerful tool for both qualitative and quantitative analysis of a vast range of biomolecules within single cells. In recent years, the integration of microfluidics and MS has gained significant attention, offering unprecedented precision and efficiency for single-cell studies. This review summarizes recent advances in microfluidic cell manipulation techniques and their integration with single-cell MS. The application scope covers multiple omics fields, including proteomics, metabolomics, lipidomics, metallomics, genomics, and transcriptomics. The review also discusses current challenges, outlines future research directions, and underscores the significance of this interdisciplinary area in biology, medicine, and environmental researches.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"196 ","pages":"Article 118630"},"PeriodicalIF":12.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145922592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Molecular design of metal complex-based luminescence probes for time-gated luminescence bioassays 用于时间门控发光生物测定的金属配合物发光探针的分子设计

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Trends in Analytical Chemistry Pub Date : 2026-02-01 Epub Date: 2025-12-24 DOI: 10.1016/j.trac.2025.118627

Yundi Huang , Bo Song , Wenzhu Zhang , Jingli Yuan

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Recent progress of ionic microporous organic networks and their composites in solid phase extraction 离子微孔有机网络及其复合材料在固相萃取中的研究进展

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Trends in Analytical Chemistry Pub Date : 2026-02-01 Epub Date: 2025-12-17 DOI: 10.1016/j.trac.2025.118615

Ting Wang , Yuan-Yuan Cui , Xi-Xi Wang , Cheng-Xiong Yang

{"title":"Recent progress of ionic microporous organic networks and their composites in solid phase extraction","authors":"Ting Wang , Yuan-Yuan Cui , Xi-Xi Wang , Cheng-Xiong Yang","doi":"10.1016/j.trac.2025.118615","DOIUrl":"10.1016/j.trac.2025.118615","url":null,"abstract":"<div><div>Ionic microporous organic networks (iMONs) represent an emerging class of functional polymeric adsorbents that synergistically integrate the structural advantages of conventional MONs with the unique properties of ionic functional groups. These advanced materials have gained significant attention in solid phase extraction (SPE) due to their exceptional characteristics, including abundant chargeable interaction sites, high specific surface area, excellent stability, and remarkable compatibility with various supporting matrices. This review systematically summarizes: (1) state-of-the-art preparation strategies of iMONs; (2) methods of iMONs composite fabrication; (3) their applications across various SPE techniques, including dispersive SPE, column SPE, magnetic SPE, stir bar sorptive extraction, and on-line SPE. Compared with commercial adsorbents, iMONs exhibit superior adsorption capacity, selectivity, and efficiency. Current challenges involve synthesis cost, environmental impact, and performance in complex real samples. Future efforts should focus on greener synthesis, systematic comparison with commercial adsorbents, and computational studies to elucidate extraction mechanisms.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"195 ","pages":"Article 118615"},"PeriodicalIF":12.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145797515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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