Trends in Analytical Chemistry最新文献_第9页

Recent Application of CRISPR/Cas in Plant Disease Detection CRISPR/Cas在植物病害检测中的最新应用

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-04-04 DOI: 10.1016/j.trac.2025.118251

Xue-Yan Gong, Zi-Han Wang, Maria Bashir, Ting Tang, Xiuhai Gan, Wen-Chao Yang

{"title":"Recent Application of CRISPR/Cas in Plant Disease Detection","authors":"Xue-Yan Gong, Zi-Han Wang, Maria Bashir, Ting Tang, Xiuhai Gan, Wen-Chao Yang","doi":"10.1016/j.trac.2025.118251","DOIUrl":"10.1016/j.trac.2025.118251","url":null,"abstract":"<div><div>Plant diseases have significant adverse impacts on the food security and biodiversity, making effective diagnosis extremely essential for efficient and sustainable plant disease management. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated protein (Cas)-based systems have emerged as powerful tools for detecting plant pathogens, due to their fast speed, and excellent accuracy. However, a comprehensive systematic review of the application of CRISPR/Cas in plant disease diagnosis is lacking, which partially hampers the practical application. This review firstly discusses the underlying principles of CRISPR/Cas-based biosensing technology. Then it highlights the significant applications in detecting plant viruses, bacteria, and fungi. Finally, it provides insightful analysis and predictions regarding the future development of the CRISPR/Cas system for diagnosing plant diseases. This review not only contributes to the advancement of plant disease diagnosis technology based on the CRISPR/Cas system, but also offers inspiration for smart plant protection and precision agriculture.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118251"},"PeriodicalIF":11.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advance in single-molecule detection and imaging 单分子检测和成像的最新进展

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-04-04 DOI: 10.1016/j.trac.2025.118252

Yun Han , Weijie Tong , Fei Ma, Chun-yang Zhang

{"title":"Recent advance in single-molecule detection and imaging","authors":"Yun Han , Weijie Tong , Fei Ma, Chun-yang Zhang","doi":"10.1016/j.trac.2025.118252","DOIUrl":"10.1016/j.trac.2025.118252","url":null,"abstract":"<div><div>Single-molecule detection and imaging have provided a powerful toolbox for exploring the molecular basis of life. In contrast to conventional ensemble measurements, single-molecule detection technique can directly observe and investigate the properties, behaviors, and interactions of individual molecules at the molecular level. The capability of measuring single biomolecules with good specificity and high accuracy is of great importance in biosensing, bioimaging, biomedical research and diseases diagnosis. Single-molecule detection technique can be used for characterizing and measuring various biomarkers due to its unique advantages of high resolution, ultrahigh sensitivity, visualization capability, and minimal sample consumption. Herein, we review the recent advance in fluorescence-, electrochemiluminescence- and surface-enhanced Raman scattering (SERS)-based single molecule detection and imaging for sensitive measurement of nucleic acids, proteins, viruses, living cells, and exosomes in clinical samples. We summarize the working principles and applications of these technologies, and discuss the current challenges and future perspectives in this field as well.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118252"},"PeriodicalIF":11.8,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143790996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances of aptamer based sensors for aflatoxins 基于适体的黄曲霉毒素传感器研究进展

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-04-03 DOI: 10.1016/j.trac.2025.118250

Meiting Kang , Qiang Zhao

引用次数: 0

Automation and high throughput sample analysis with various platforms in microextraction techniques: A need for ecofriendly, green, and cost-effective sample preparation approaches – A review 微萃取技术中各种平台的自动化和高通量样品分析：对环保，绿色和具有成本效益的样品制备方法的需求-综述

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-04-02 DOI: 10.1016/j.trac.2025.118247

Wajid Ali Khan , S. Amir Hossein Fallah , Saeid Moltajihagh , Fatemeh Hamdi , Fatemeh Alipour , Afsaneh Mollahosseini , Grzegorz Boczkaj

{"title":"Automation and high throughput sample analysis with various platforms in microextraction techniques: A need for ecofriendly, green, and cost-effective sample preparation approaches – A review","authors":"Wajid Ali Khan , S. Amir Hossein Fallah , Saeid Moltajihagh , Fatemeh Hamdi , Fatemeh Alipour , Afsaneh Mollahosseini , Grzegorz Boczkaj","doi":"10.1016/j.trac.2025.118247","DOIUrl":"10.1016/j.trac.2025.118247","url":null,"abstract":"<div><div>Popularization of green analytical chemistry (GAC) encouraged miniaturization of sample preparation scale – with preference to microextraction scale methods and automation by platforms to achieve high throughput, reduce energy costs, minimize hazardous chemical disposal, and shorten extraction time. It improves the sample throughput, extraction efficiency, sensitivity, reproducibility, and greenness of the developed method. To achieve these objectives, automatic platforms for high-throughput liquid-phase microextraction (LPME) and solid-phase extraction/microextraction (SPE/SPME) were developed. Among the reported platforms, the 96-well plate, pipette tip, lab-in-syringe (LIS), and microfluidic devices receive significant attention. This review describes the advantages of each platform with respect to green chemistry principles. In addition, the challenges associated with each platform are critically evaluated. Moreover, the review highlights how the use of these platforms can minimize waste disposal, energy consumption, and reduce the hazardous effects. Furthermore, a comparative assessment of the above mentioned platforms in terms of green microextraction techniques has been discussed.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118247"},"PeriodicalIF":11.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Innovative Nanozyme-Based Detection Methods for Biomarkers in Interstitial Fluid 创新的基于纳米酶的间质液生物标志物检测方法

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-04-02 DOI: 10.1016/j.trac.2025.118248

Jiuhong Zhao, Junjie Tian, Xiaoyu Zhang, Jinnan Zhao, Guixia Ling, Peng Zhang

{"title":"Innovative Nanozyme-Based Detection Methods for Biomarkers in Interstitial Fluid","authors":"Jiuhong Zhao, Junjie Tian, Xiaoyu Zhang, Jinnan Zhao, Guixia Ling, Peng Zhang","doi":"10.1016/j.trac.2025.118248","DOIUrl":"10.1016/j.trac.2025.118248","url":null,"abstract":"<div><div>The detection of biomarkers can timely detect abnormalities and provide appropriate treatment for patients, which effectively improves the prognosis of the disease. Interstitial fluid (ISF) contains a variety of biomarkers similar to other body fluids. The detection of biomarkers in ISF can not only reflect the physiological and pathological changes in the body immediately but also reduce the physical harm to patients. Nanomaterials are low-cost, more stable, easy-preparation, and less susceptible to environmental factors, which have similar catalytic activities and electrochemical properties. Through the combination of nanomaterials with ISF analysis, it can successfully achieve minimally-invasive detection of biomarkers, which not only improves the accuracy and sensitivity of detection but also significantly reduces patient suffering. The non-enzymatic detection methods for biomarkers in ISF play a crucial role in the early diagnosis of diseases, especially diabetes, renal disease, and cardiovascular disease, which will hold implications for the development of future clinical diagnosis.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118248"},"PeriodicalIF":11.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous framework materials for mycotoxin detection: From synthesis to application 真菌毒素检测用多孔框架材料：从合成到应用

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-04-01 DOI: 10.1016/j.trac.2025.118249

Mengxiang Zhang , Weiqiang Li , Jin Huang , Xueying Tao , Hengyi Xu

{"title":"Porous framework materials for mycotoxin detection: From synthesis to application","authors":"Mengxiang Zhang , Weiqiang Li , Jin Huang , Xueying Tao , Hengyi Xu","doi":"10.1016/j.trac.2025.118249","DOIUrl":"10.1016/j.trac.2025.118249","url":null,"abstract":"<div><div>Mycotoxins have posed a serious threat to human health and agricultural economy, which lead to an urgent need for alleviating and improving the tough problem caused by mycotoxins. Therefore, it is significant to establish facile, sensitive and accurate methods for the detection of mycotoxins considering the shortcomings of existing methods. Porous framework materials (PFMs) are a promising class of nanomaterials with excellent properties, and have demonstrated strong application potential in adsorption separation and analysis sensing, which could enhance mycotoxins detection. This review summarizes the research progress of synthetic PFMs and their application in mycotoxin detection. The principles and details of various synthesis methods are emphatically introduced, and further elaborates on the application scenarios, including separation and sensing. The transition towards green synthesis methods and eliminating harmful organic solvents is a growing trend. Future works could focus on developing precise synthesis based on green solvents and simultaneous detection of multiple analytes.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118249"},"PeriodicalIF":11.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in DNA damage detection: Current progress, challenges, and future directions DNA损伤检测的进展：当前进展、挑战和未来方向

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-03-27 DOI: 10.1016/j.trac.2025.118246

Heeseok Kang , Hyung Joon Park , Jieun Kang , Yuna Hwang , YongJin Lee , Sang-Uk Park , Jeongkyu Kim , Kwan Hyi Lee , Sunbok Jang

{"title":"Advances in DNA damage detection: Current progress, challenges, and future directions","authors":"Heeseok Kang , Hyung Joon Park , Jieun Kang , Yuna Hwang , YongJin Lee , Sang-Uk Park , Jeongkyu Kim , Kwan Hyi Lee , Sunbok Jang","doi":"10.1016/j.trac.2025.118246","DOIUrl":"10.1016/j.trac.2025.118246","url":null,"abstract":"<div><div>DNA is much continuously exposed to endogenous and exogenous agents that induce damage, potentially leading to genomic instability, disease development, and aging. Detecting DNA damage and understanding associated repair mechanisms are essential for advancing diagnostic techniques, biosensors, and therapeutic interventions. Since DNA damage varies, the desired measurement approach differs, highlighting the need for an unbiased exploration of DNA damage biomarkers, along with a critical analysis of various quantification methods. Therefore, this review aims to provide a comprehensive overview of current methodologies for measuring DNA damage, focusing on direct detection of DNA lesions and indirect measurement of repair enzymes. We discuss various analytical, biochemical, and imaging techniques, identify the limitations in existing technologies and suggest future directions. This review emphasizes the growing need for advanced tools to measure DNA damage, which is set to play a transformative role in early disease detection, optimizing treatments, and supporting safe human space exploration.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118246"},"PeriodicalIF":11.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-throughput CRISPR approaches: New solutions for challenging problems 高通量CRISPR方法：挑战性问题的新解决方案

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-03-27 DOI: 10.1016/j.trac.2025.118245

Gargi Bindal , Chitra Seetharam Misra , Mandeep Singh , Shyam Sunder Rangu , Neha Pandey , Devashish Rath

{"title":"High-throughput CRISPR approaches: New solutions for challenging problems","authors":"Gargi Bindal , Chitra Seetharam Misra , Mandeep Singh , Shyam Sunder Rangu , Neha Pandey , Devashish Rath","doi":"10.1016/j.trac.2025.118245","DOIUrl":"10.1016/j.trac.2025.118245","url":null,"abstract":"<div><div>The CRISPR technology is a formidable tool that allows precise interrogation and manipulation of genomes, at unprecedented throughput. The CRISPR technology integrated with other high-throughput methods opens new opportunities for research and development in different areas and presents new solutions to challenges in health, agriculture, and biotechnology. CRISPR-based functional screens have helped in identifying potential drug targets for both infectious and non-infectious diseases, revealed mechanisms involved in antibiotic resistance, host-pathogen interactions, cancer progression and response to drugs. CRISPR screening has facilitated engineering of biosynthetic pathways and optimization of cell factories for pharmaceutical and biofuel production. CRISPR-based massively parallel genome editing has enabled increase in crop yield and tolerance to abiotic/biotic stresses with the consequent increase of fitness and adaptability of the crop. This review also highlights the innovative approaches integrating CRISPR and other high-throughput techniques to find new solutions to long-standing and complex problems in biological research.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118245"},"PeriodicalIF":11.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143858934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sample Preparation of Polar Metabolites in Biological Samples: Methodologies and Technological Insights 生物样品中极性代谢物的样品制备：方法和技术见解

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-03-26 DOI: 10.1016/j.trac.2025.118244

Xue-Man Dong , Frederik André Hansen , Pu Wu , Yelu Wu , Sile He , Weiyang Tang

{"title":"Sample Preparation of Polar Metabolites in Biological Samples: Methodologies and Technological Insights","authors":"Xue-Man Dong , Frederik André Hansen , Pu Wu , Yelu Wu , Sile He , Weiyang Tang","doi":"10.1016/j.trac.2025.118244","DOIUrl":"10.1016/j.trac.2025.118244","url":null,"abstract":"<div><div>Polar metabolites in biological samples are vital for various biochemical processes and serve as key indicators in numerous clinical and experimental investigations. However, the inherent complexity of biological matrices and the strong polarity of metabolites present significant challenges in sample preparation, resulting in inefficient extraction and matrix effects. Therefore, continuous advances in pre-treatment methodologies are crucial for improving sensitivity, reproducibility, and overall analytical performance. This review provides a comprehensive overview of advancements in analytical preparation techniques for polar metabolites in biological samples since 2020. It examines the strengths and limitations of these methods, focusing on their applications in extracting and analyzing diverse classes of polar metabolites. By exploring the latest trends and developments in sample processing, this review aims to assist researchers in selecting appropriate techniques for efficient and reliable metabolite analysis, ultimately improving the accuracy and depth of biochemical research.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118244"},"PeriodicalIF":11.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Halloysite-based sorbents: Synthesis, characterization, and applications in sample preparation 以高岭土为基础的吸附剂：合成、表征和在样品制备中的应用

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-03-22 DOI: 10.1016/j.trac.2025.118241

Omid Mazloumi, Fatemeh Zahra Biabanpour, Saeed Nojavan

{"title":"Halloysite-based sorbents: Synthesis, characterization, and applications in sample preparation","authors":"Omid Mazloumi, Fatemeh Zahra Biabanpour, Saeed Nojavan","doi":"10.1016/j.trac.2025.118241","DOIUrl":"10.1016/j.trac.2025.118241","url":null,"abstract":"<div><div>Halloysite nanotubes (HNTs), naturally occurring aluminosilicate clay minerals, possess unique structural and physicochemical properties that make them highly promising sorbents for solid-based extraction methods. Their tubular structure, high surface area, and inherent adsorption capacity enable efficient capture of analytes from complex matrices. This review comprehensively examines the application of HNTs in solid-phase extraction (SPE) and its miniaturized variants, such as solid-phase microextraction (SPME) or micro SPE (μ-SPE), focusing on their use in isolating analytes from environmental, beverage, food, and biological samples. Key aspects covered include the synthesis and modification of HNT-based composites to enhance extraction selectivity and efficiency, the assessment of the environmental impact and greenness of the extraction methodologies, and the characterization techniques used to elucidate the structure-property relationships of the HNT-based materials. The review highlights the significant potential of HNTs to advance sample preparation technologies by offering sustainable and efficient extraction processes across a broad range of analytical disciplines.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118241"},"PeriodicalIF":11.8,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143739225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0