High-throughput CRISPR approaches: New solutions for challenging problems

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-03-27 DOI:10.1016/j.trac.2025.118245

Gargi Bindal , Chitra Seetharam Misra , Mandeep Singh , Shyam Sunder Rangu , Neha Pandey , Devashish Rath

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Abstract

The CRISPR technology is a formidable tool that allows precise interrogation and manipulation of genomes, at unprecedented throughput. The CRISPR technology integrated with other high-throughput methods opens new opportunities for research and development in different areas and presents new solutions to challenges in health, agriculture, and biotechnology. CRISPR-based functional screens have helped in identifying potential drug targets for both infectious and non-infectious diseases, revealed mechanisms involved in antibiotic resistance, host-pathogen interactions, cancer progression and response to drugs. CRISPR screening has facilitated engineering of biosynthetic pathways and optimization of cell factories for pharmaceutical and biofuel production. CRISPR-based massively parallel genome editing has enabled increase in crop yield and tolerance to abiotic/biotic stresses with the consequent increase of fitness and adaptability of the crop. This review also highlights the innovative approaches integrating CRISPR and other high-throughput techniques to find new solutions to long-standing and complex problems in biological research.

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高通量CRISPR方法：挑战性问题的新解决方案

CRISPR技术是一种强大的工具，可以以前所未有的吞吐量对基因组进行精确的询问和操纵。CRISPR技术与其他高通量方法相结合，为不同领域的研究和开发开辟了新的机会，并为卫生、农业和生物技术领域的挑战提供了新的解决方案。基于crispr的功能筛选有助于确定感染性和非感染性疾病的潜在药物靶点，揭示抗生素耐药性、宿主-病原体相互作用、癌症进展和对药物反应的机制。CRISPR筛选促进了生物合成途径的工程设计和优化用于制药和生物燃料生产的细胞工厂。基于crispr的大规模平行基因组编辑使作物产量和对非生物/生物胁迫的耐受性得以提高，从而提高了作物的适应性和适应性。这篇综述还强调了整合CRISPR和其他高通量技术的创新方法，为生物学研究中长期存在的复杂问题找到新的解决方案。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.