Recent Application of CRISPR/Cas in Plant Disease Detection

IF 11.8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Trends in Analytical Chemistry Pub Date : 2025-04-04 DOI:10.1016/j.trac.2025.118251

Xue-Yan Gong, Zi-Han Wang, Maria Bashir, Ting Tang, Xiuhai Gan, Wen-Chao Yang

{"title":"Recent Application of CRISPR/Cas in Plant Disease Detection","authors":"Xue-Yan Gong, Zi-Han Wang, Maria Bashir, Ting Tang, Xiuhai Gan, Wen-Chao Yang","doi":"10.1016/j.trac.2025.118251","DOIUrl":null,"url":null,"abstract":"<div><div>Plant diseases have significant adverse impacts on the food security and biodiversity, making effective diagnosis extremely essential for efficient and sustainable plant disease management. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated protein (Cas)-based systems have emerged as powerful tools for detecting plant pathogens, due to their fast speed, and excellent accuracy. However, a comprehensive systematic review of the application of CRISPR/Cas in plant disease diagnosis is lacking, which partially hampers the practical application. This review firstly discusses the underlying principles of CRISPR/Cas-based biosensing technology. Then it highlights the significant applications in detecting plant viruses, bacteria, and fungi. Finally, it provides insightful analysis and predictions regarding the future development of the CRISPR/Cas system for diagnosing plant diseases. This review not only contributes to the advancement of plant disease diagnosis technology based on the CRISPR/Cas system, but also offers inspiration for smart plant protection and precision agriculture.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"189 ","pages":"Article 118251"},"PeriodicalIF":11.8000,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Analytical Chemistry","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165993625001190","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Plant diseases have significant adverse impacts on the food security and biodiversity, making effective diagnosis extremely essential for efficient and sustainable plant disease management. Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated protein (Cas)-based systems have emerged as powerful tools for detecting plant pathogens, due to their fast speed, and excellent accuracy. However, a comprehensive systematic review of the application of CRISPR/Cas in plant disease diagnosis is lacking, which partially hampers the practical application. This review firstly discusses the underlying principles of CRISPR/Cas-based biosensing technology. Then it highlights the significant applications in detecting plant viruses, bacteria, and fungi. Finally, it provides insightful analysis and predictions regarding the future development of the CRISPR/Cas system for diagnosing plant diseases. This review not only contributes to the advancement of plant disease diagnosis technology based on the CRISPR/Cas system, but also offers inspiration for smart plant protection and precision agriculture.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Trends in Analytical Chemistry 化学-分析化学

CiteScore

20.00

自引率

4.60%

发文量

257

审稿时长

3.4 months

期刊介绍： TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.