CAHIERS DE NUTRITION ET DE DIETETIQUE最新文献

{"title":"SIRT1 -1400T>C Polymorphism (rs12778366) and metabolic-inflammatory variability in women with systemic lupus erythematosus: Perspectives for personalized nutrition","authors":"Amanda Alves Ribeiro, Beatriz Garcia de Carvalho, Jhulia Caroline Nunes Leal da Mota, Lucas Moura Carvalho, José Alfredo Martinez, Eduardo Ferreira Borba, Carolina Nicoletti Ferreira Fino","doi":"10.1016/j.cnd.2026.08.077","DOIUrl":"10.1016/j.cnd.2026.08.077","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate the association between the SIRT1 -1400T>C polymorphism (rs12778366) and body composition parameters as well as inflammatory biomarkers in women with SLE.</div></div><div>This study aimed to investigate the association between the SIRT1 -1400T>C polymorphism (rs12778366) and body composition parameters as well as inflammatory biomarkers in women with SLE.</div><div><h3>Methods</h3><div>This exploratory study included 121 women diagnosed with SLE and followed at the Adult Lupus Outpatient Clinic of the Rheumatology Division, Hospital das Clínicas, Faculty of Medicine, University of São Paulo. Participants were stratified according to the TT, CT, and CC genotypes of the -1400T>C polymorphism. The evaluated parameters included body mass index (BMI), waist circumference (WC), absolute lean mass (LM), percentage of lean mass, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and disease activity assessed by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Data normality was assessed using the Shapiro–Wilk test. Parametric variables were analyzed using one-way ANOVA, whereas nonparametric variables were compared using the Kruskal–Wallis test. Statistical significance was set at <em>P</em> <!--><<!--> <!-->0.05. All study procedures were conducted following approval by the Research Ethics Committee of the Hospital das Clínicas, Faculty of Medicine, University of São Paulo (HC-FMUSP), under CAAE No. 86418824.6.0000.0068.</div></div><div>This exploratory study included 121 women diagnosed with SLE and followed at the Adult Lupus Outpatient Clinic of the Rheumatology Division, Hospital das Clínicas, Faculty of Medicine, University of São Paulo. Participants were stratified according to the TT, CT, and CC genotypes of the -1400T>C polymorphism. The evaluated parameters included body mass index (BMI), waist circumference (WC), absolute lean mass (LM), percentage of lean mass, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and disease activity assessed by the Systemic Lupus Erythematosus Disease Activity Index (SLEDAI). Data normality was assessed using the Shapiro–Wilk test. Parametric variables were analyzed using one-way ANOVA, whereas nonparametric variables were compared using the Kruskal–Wallis test. Statistical significance was set at <em>P</em> <!--><<!--> <!-->0.05. All study procedures were conducted following approval by the Research Ethics Committee of the Hospital das Clínicas, Faculty of Medicine, University of São Paulo (HC-FMUSP), under CAAE No. 86418824.6.0000.0068.</div><div><h3>Results</h3><div>No statistically significant differences were observed among genotypes for BMI (<em>P</em> <!-->=<!--> <!-->0.802), WC (<em>P</em> <!-->=<!--> <!-->0.252), LM (<em>P</em> <!-->=<!--> <!-->0.094), percentage of lean mass (<em>P</em> <!-->=<!--> <!-->0.629), CRP (<em>P</em> <!-->=<!--> <!-->0.070), ESR (<em>P</em> <!-->=<!--> <","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e42"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omic predictors of BMI trajectories in adults with MASLD undergoing personalized dietary treatment","authors":"Carolynne Martins Teixeira, Itziar Abete, Fermin I. Milagro, José I. Herrero, Mariana Elorz, Josep A. Tur, Jose Alfredo Martínez, Helen Hermana Miranda Hermsdorff, M. Angeles Zulet","doi":"10.1016/j.cnd.2026.08.081","DOIUrl":"10.1016/j.cnd.2026.08.081","url":null,"abstract":"<div><h3>Objective</h3><div>To identify Body Mass Index (BMI) trajectories over 24 months and evaluate the ability of multi-omic biomarkers to discriminate individuals according to dietary intervention response patterns.</div></div><div>To identify Body Mass Index (BMI) trajectories over 24 months and evaluate the ability of multi-omic biomarkers to discriminate individuals according to dietary intervention response patterns.</div><div><h3>Methods</h3><div>Data were obtained from the Fatty Liver in Obesity (FLiO) randomized controlled trial, involving 98 adults with overweight/obesity and diagnosed with Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). Participants underwent a 30% caloric restriction and were randomized to one of two dietary interventions (FLiO or American Heart Association [AHA] diet). BMI trajectories over 24 months were identified using Latent Class Growth Modeling (LCGM) to classify participants according to response patterns. A Polygenic Risk Score (PRS) was calculated based on 35 SNPs across 11 candidate genes by summing risk alleles. Gut microbiota composition was analyzed by shotgun sequencing. Genera significantly associated with BMI trajectories in logistic regression analyses were selected as predictors for microbiota-based models. Predictive performance was assessed using the Area Under the Receiver Operating Characteristic Curve (AUC), comparing favorable versus unfavorable trajectory patterns. The FLiO study was approved by the Ethics Committee of the University of Navarra (54/2015) and registered with Clinical Trials (FLiO: Fatty Liver on Obesity study; <span><span>NCT03183193</span><svg><path></path></svg></span>).</div></div><div>Data were obtained from the Fatty Liver in Obesity (FLiO) randomized controlled trial, involving 98 adults with overweight/obesity and diagnosed with Metabolic Dysfunction-Associated Steatotic Liver Disease (MASLD). Participants underwent a 30% caloric restriction and were randomized to one of two dietary interventions (FLiO or American Heart Association [AHA] diet). BMI trajectories over 24 months were identified using Latent Class Growth Modeling (LCGM) to classify participants according to response patterns. A Polygenic Risk Score (PRS) was calculated based on 35 SNPs across 11 candidate genes by summing risk alleles. Gut microbiota composition was analyzed by shotgun sequencing. Genera significantly associated with BMI trajectories in logistic regression analyses were selected as predictors for microbiota-based models. Predictive performance was assessed using the Area Under the Receiver Operating Characteristic Curve (AUC), comparing favorable versus unfavorable trajectory patterns. The FLiO study was approved by the Ethics Committee of the University of Navarra (54/2015) and registered with Clinical Trials (FLiO: Fatty Liver on Obesity study; <span><span>NCT03183193</span><svg><path></path></svg></span>).</div><div><h3>Results</h3><div>LCGM identified three distinct","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e44"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between intestinal dysbiosis risk and anthropometric and biochemical parameters in overweight and obese individuals","authors":"Kamile Abreu Inacio, Gustavo Braga, Geovana Firmo Pezin, Samile Peteli Cardoso, Fabiana de Cássia Carvalho Oliveira, André Gustavo Vasconcelos Costa, Maria das Graças Vaz Tostes, Mirelle Lomar Viana","doi":"10.1016/j.cnd.2026.08.089","DOIUrl":"10.1016/j.cnd.2026.08.089","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the association between the risk of intestinal dysbiosis and body mass index (BMI), body fat percentage, LDL cholesterol levels, and serum cortisol levels in individuals with overweight or obesity.</div></div><div>To investigate the association between the risk of intestinal dysbiosis and body mass index (BMI), body fat percentage, LDL cholesterol levels, and serum cortisol levels in individuals with overweight or obesity.</div><div><h3>Methods</h3><div>This is a cross-sectional observational study presenting preliminary results from a larger project (PPSUS), which is expected to evaluate 272 individuals receiving care at Primary Health Care Units in Alegre, ES, Brazil. Twenty-three participants underwent an indirect dysbiosis assessment questionnaire (DYS-R), anthropometric assessment, biochemical tests, and bioimpedance analysis (InBody®). Stata software was used for analyses. Data normality was assessed using the Shapiro–Wilk test. Correlations were analyzed using Pearson's correlation test, and one-way analysis of variance (ANOVA) was used to compare means among independent groups, with significance set at <em>P</em> <!--><<!--> <!-->0.05. This study was approved by the Human Research Ethics Committee under protocol number No. 7.357.997.</div></div><div>This is a cross-sectional observational study presenting preliminary results from a larger project (PPSUS), which is expected to evaluate 272 individuals receiving care at Primary Health Care Units in Alegre, ES, Brazil. Twenty-three participants underwent an indirect dysbiosis assessment questionnaire (DYS-R), anthropometric assessment, biochemical tests, and bioimpedance analysis (InBody®). Stata software was used for analyses. Data normality was assessed using the Shapiro–Wilk test. Correlations were analyzed using Pearson's correlation test, and one-way analysis of variance (ANOVA) was used to compare means among independent groups, with significance set at <em>P</em> <!--><<!--> <!-->0.05. This study was approved by the Human Research Ethics Committee under protocol number No. 7.357.997.</div><div><h3>Results</h3><div>The mean DYS-R score was 19.09<!--> <!-->±<!--> <!-->5.12. The mean values for BMI, body fat percentage, LDL cholesterol, and serum cortisol were 33.48<!--> <!-->±<!--> <!-->7.22<!--> <!-->kg/m<sup>2</sup>, 43.54<!--> <!-->±<!--> <!-->8.49%, 112.32<!--> <!-->±<!--> <!-->31.00<!--> <!-->mg/dL, and 12.59<!--> <!-->±<!--> <!-->5.33<!--> <!-->μg/dL, respectively. No significant correlations were observed between DYS-R scores and BMI (<em>P</em> <!-->=<!--> <!-->0.82), body fat percentage (<em>P</em> <!-->=<!--> <!-->0.99), LDL cholesterol (<em>P</em> <!-->=<!--> <!-->0.16), or cortisol levels (<em>P</em> <!-->=<!--> <!-->0.50). Likewise, no significant divergences were found in BMI (<em>P</em> <!-->=<!--> <!-->0.86), body fat percentage (<em>P</em> <!-->=<!--> <!-->0.83), LDL cholesterol (<em>P</em> <!-->=<!--> <!-->0.27), or cor","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e48"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polygenic risk score is associated with metabolically unhealthy obesity: A secondary analysis from an energy-restricted intervention (Brazilian Nuts Study)","authors":"Darlene Larissa de Souza Vilela, Karla Pereira Balbino, Ana Cláudia Pelissari Kravchychyn, Josefina Bressan, Helen Hermana Miranda Hermsdorff","doi":"10.1016/j.cnd.2026.08.087","DOIUrl":"10.1016/j.cnd.2026.08.087","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the association between a polygenic risk score (PRS) and metabolic obesity phenotypes in Brazilian adults.</div></div><div>To evaluate the association between a polygenic risk score (PRS) and metabolic obesity phenotypes in Brazilian adults.</div><div><h3>Methods</h3><div>This secondary cross-sectional analysis utilized baseline data from the “Brazilian Nuts Study” Phase 3, a randomized controlled trial (ReBec registration number RBR8xzkyp2). All volunteers signed the Informed Consent Form (Ethics Committee's opinion No. 4.543.541/CEPH/UFV). The study included 92 adults, both sexes, with overweight (BMI 27–29.9<!--> <!-->kg/m<sup>2</sup>) or obesity (BMI<!--> <!-->≥<!--> <!-->30<!--> <!-->kg/m<sup>2</sup>), increased waist circumference (≥<!--> <!-->80<!--> <!-->cm for women and ≥<!--> <!-->90<!--> <!-->cm for men), and elevated body fat (><!--> <!-->30% for women and ><!--> <!-->20% for men). Participants were classified as having metabolically healthy obesity (MHO) with<!--> <!-->≤<!--> <!-->1 metabolic abnormality and as having metabolically unhealthy obesity (MUO) with<!--> <!-->≥<!--> <!-->2 metabolic abnormalities. Metabolic abnormalities included impaired fasting glucose/insulin resistance, dyslipidemia (low HDL cholesterol and/or elevated triglycerides), and hypertension. The PRS was composed of four single-nucleotide polymorphisms (SNPs) (FTO rs9939609; FTO rs1121980; MC4R rs17782313; LEP rs7799039). An unweighted polygenic risk score (dominant model). Defined as the sum of risk alleles, individuals carrying 1 or 2 risk alleles are assigned a value of 1, yielding a PRS ranging from 0 to 4. Logistic regression was performed using Stata® 17.</div></div><div>This secondary cross-sectional analysis utilized baseline data from the “Brazilian Nuts Study” Phase 3, a randomized controlled trial (ReBec registration number RBR8xzkyp2). All volunteers signed the Informed Consent Form (Ethics Committee's opinion No. 4.543.541/CEPH/UFV). The study included 92 adults, both sexes, with overweight (BMI 27–29.9<!--> <!-->kg/m<sup>2</sup>) or obesity (BMI<!--> <!-->≥<!--> <!-->30<!--> <!-->kg/m<sup>2</sup>), increased waist circumference (≥<!--> <!-->80<!--> <!-->cm for women and ≥<!--> <!-->90<!--> <!-->cm for men), and elevated body fat (><!--> <!-->30% for women and ><!--> <!-->20% for men). Participants were classified as having metabolically healthy obesity (MHO) with<!--> <!-->≤<!--> <!-->1 metabolic abnormality and as having metabolically unhealthy obesity (MUO) with<!--> <!-->≥<!--> <!-->2 metabolic abnormalities. Metabolic abnormalities included impaired fasting glucose/insulin resistance, dyslipidemia (low HDL cholesterol and/or elevated triglycerides), and hypertension. The PRS was composed of four single-nucleotide polymorphisms (SNPs) (FTO rs9939609; FTO rs1121980; MC4R rs17782313; LEP rs7799039). An unweighted polygenic risk score (dominant model). Defined as the sum of risk alleles, indivi","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e47"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics integration for the identification of key genes associated with pancreatic islet function in type 2 diabetes","authors":"Viridiana Basaldúa-Maciel, Fernando Martínez-Esquivias, Juan Manuel Guzmán-Flores","doi":"10.1016/j.cnd.2026.08.006","DOIUrl":"10.1016/j.cnd.2026.08.006","url":null,"abstract":"<div><h3>Objective</h3><div>To identify key molecular alterations and hub genes associated with pancreatic islet function in type 2 diabetes (T2D) through a multi-omics integration approach.</div></div><div>To identify key molecular alterations and hub genes associated with pancreatic islet function in type 2 diabetes (T2D) through a multi-omics integration approach.</div><div><h3>Methods</h3><div>Genes associated with T2D were retrieved from DisGeNET, MalaCards, and the Comparative Toxicogenomics Database. Transcriptomic data from human pancreatic islets were obtained from the Gene Expression Omnibus (GEO), while proteomic data were collected from the Omics Discovery Index. Multi-omics datasets were integrated using OmicsNet 2.0 to construct a protein-protein interaction network. Functional enrichment analyses were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Hub genes were identified based on their connectivity within the interaction network. This study was conducted exclusively using publicly available, de-identified datasets. Therefore, approval from a Research Ethics Committee was not required according to institutional and applicable ethical guidelines.</div></div><div>Genes associated with T2D were retrieved from DisGeNET, MalaCards, and the Comparative Toxicogenomics Database. Transcriptomic data from human pancreatic islets were obtained from the Gene Expression Omnibus (GEO), while proteomic data were collected from the Omics Discovery Index. Multi-omics datasets were integrated using OmicsNet 2.0 to construct a protein-protein interaction network. Functional enrichment analyses were performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Hub genes were identified based on their connectivity within the interaction network. This study was conducted exclusively using publicly available, de-identified datasets. Therefore, approval from a Research Ethics Committee was not required according to institutional and applicable ethical guidelines.</div><div><h3>Results</h3><div>The integration of 3,260 genes, 6,918 mRNA, and 92 proteins revealed biological processes associated with metabolic regulation, cellular metabolism, and enzyme binding. Functional enrichment analyses highlighted mechanisms involved in mitochondrial metabolism, intracellular signaling, transcriptional regulation, and insulin secretion. Enriched pathways included Ras/MAPK signaling, basal transcription factors, gap junctions, and oxidative phosphorylation. Network analysis identified ten highly connected hub genes: <em>SRC</em>, <em>TP53</em>, <em>AKT1</em>, <em>CTNNB1</em>, <em>EP300</em>, <em>MAPK1</em>, <em>MAPK3</em>, <em>CREBBP</em>, <em>EGFR</em>, and <em>HSP90AA1</em>. These genes are associated with key biological functions involved in glucose uptake, insulin receptor signaling, energy metabolism, and pancreatic B-cell survival.</div></div><div>The integration of 3,260 genes, 6","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e5"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-of-1 multi-omics precision nutrition modeling after an energy-restricted anti-inflammatory intervention in a subject with obesity","authors":"Amanda Cuevas-Sierra, Andrea Higuera-Gómez, Carolina Nicoletti, María del Carmen López de las Hazas, Joao Tomé-Carneiro, Alberto Dávalos, J. Alfredo Martínez","doi":"10.1016/j.cnd.2026.08.038","DOIUrl":"10.1016/j.cnd.2026.08.038","url":null,"abstract":"<div><h3>Background/objective</h3><div>N-of-1 methodologies have emerged as a promising precision nutrition framework, merging clinical and multi-omics data to characterize individual metabolic responses and to follow dynamic biological pathways. This approach offers an opportunity to understand obesity physiopathology and to advance in personalized disease management. The objective was to investigate the physiological response to an energy-restricted anti-inflammatory diet in obesity through the integration of clinical and multi-omics data within an N-of-1 precision nutrition model.</div></div><div>N-of-1 methodologies have emerged as a promising precision nutrition framework, merging clinical and multi-omics data to characterize individual metabolic responses and to follow dynamic biological pathways. This approach offers an opportunity to understand obesity physiopathology and to advance in personalized disease management. The objective was to investigate the physiological response to an energy-restricted anti-inflammatory diet in obesity through the integration of clinical and multi-omics data within an N-of-1 precision nutrition model.</div><div><h3>Methods</h3><div>A 76-year-old man with obesity (BMI 31.9<!--> <!-->kg/m<sup>2</sup>) underwent a 32-week anti-inflammatory intervention involving 30% energy restriction (ethic committee no.PI 164–21). The resulting data were compared with those from a healthy normal-weight subject following a N-1 approach. Conventional anthropometric and biochemical assessments were performed before and after the intervention using validated methods. Multi-omics characterization included gut microbiota by 16S rRNA gene sequencing, targeted serum metabolomics, genotyping, miRNA transcriptomic, and DNA methylation. MEDAS-14 validated questionnaire was used to determine diet adherence.</div></div><div>A 76-year-old man with obesity (BMI 31.9<!--> <!-->kg/m<sup>2</sup>) underwent a 32-week anti-inflammatory intervention involving 30% energy restriction (ethic committee no.PI 164–21). The resulting data were compared with those from a healthy normal-weight subject following a N-1 approach. Conventional anthropometric and biochemical assessments were performed before and after the intervention using validated methods. Multi-omics characterization included gut microbiota by 16S rRNA gene sequencing, targeted serum metabolomics, genotyping, miRNA transcriptomic, and DNA methylation. MEDAS-14 validated questionnaire was used to determine diet adherence.</div><div><h3>Results</h3><div>The intervention resulted in body weight decreased by 4.4%, waist circumference by 4.2%, systolic blood pressure by 15.0%, lipid metabolism variables decreased between 10.1–15.7%, and glucose metabolism markers decreased between 2.8–40%. Adherence to the Mediterranean diet increased by 22.2%, while C-reactive protein decreased by 50%. Genetic profiling revealed high susceptibility to appetite dysregulation (90th percentile), impaired liver f","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Pages e21-e22"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Obesity-induced modulation of MFSD2a in hypothalamic nuclei","authors":"Camila Venturini Ayres Cunha, Leandro Kansuke Oharomari, Letícia Maria Paula da Silva, Danillo Henrique Travensolo Buoro, Maria Luiza Salmazo Gatz, Talitha Silva Meneguelli, Eduardo Rochete Ropelle, José Rodrigo Pauli, Adelino Sanchez Ramos da Silva, Dennys Esper Cintra","doi":"10.1016/j.cnd.2026.08.088","DOIUrl":"10.1016/j.cnd.2026.08.088","url":null,"abstract":"<div><h3>Background</h3><div>MFSD2a is the primary omega-3 (ω3) fatty acid transporter from the blood to the central nervous system, which also contributes to the maintenance of blood-brain barrier (BBB) permeability. Studies have shown that the BBB is heterogeneous across brain regions, leading to differences in its properties. Previously, we demonstrated that total hypothalamic MFSD2a content is changed under obesity induced by high-fat food intake. Specifically, immune-guided histology suggested that this enhancement occurred differently in hypothalamic zones, which could differently impact the control of food intake and energy expenditure. However, no further evidence exists regarding how obesity specifically alters MFSD2a in each hypothalamic nucleus, nor how transcriptional factors are involved in it. Some studies suggest that peroxisome proliferator-activated receptors (PPARs) may regulate MFSD2a transcription; however, this has not yet been conclusively confirmed.</div></div><div>MFSD2a is the primary omega-3 (ω3) fatty acid transporter from the blood to the central nervous system, which also contributes to the maintenance of blood-brain barrier (BBB) permeability. Studies have shown that the BBB is heterogeneous across brain regions, leading to differences in its properties. Previously, we demonstrated that total hypothalamic MFSD2a content is changed under obesity induced by high-fat food intake. Specifically, immune-guided histology suggested that this enhancement occurred differently in hypothalamic zones, which could differently impact the control of food intake and energy expenditure. However, no further evidence exists regarding how obesity specifically alters MFSD2a in each hypothalamic nucleus, nor how transcriptional factors are involved in it. Some studies suggest that peroxisome proliferator-activated receptors (PPARs) may regulate MFSD2a transcription; however, this has not yet been conclusively confirmed.</div><div><h3>Objective</h3><div>Evaluate the modulation of MFSD2a by diet-induced obesity in the different hypothalamic nuclei, and the participation of PPARs in its transcription.</div></div><div>Evaluate the modulation of MFSD2a by diet-induced obesity in the different hypothalamic nuclei, and the participation of PPARs in its transcription.</div><div><h3>Methods</h3><div>Obesity was induced in mice fed with a high-fat diet for 12 weeks (35% w/w fat) ad libitum. Using the immunofluorescence and microdissection technique, MFSD2a content of arcuate, paraventricular, ventromedial, and lateral hypothalamic nuclei was mapped and quantified. To determine the role of PPAR in hypothalamic MFSD2a transcription, oral PPARα and PPARγ pharmacological agonists were used, as well as PPARα knockout mice, to evaluate <em>MFSD2a</em> gene expression and content. All experimental procedures were approved by the local animal ethics committee (protocols 6721-1/2025 and 6801-1/2025).</div></div><div>Obesity was induced in mice fed with a hig","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Pages e47-e48"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive potential of soursop (Annona muricata L.) by-products: Antioxidant properties and effects on toxicity and oxidative stress in Caenorhabditis elegans","authors":"Valéria Silva de Lana, Marcella Duarte Villas Mishima, Douglas Correia de Souza, Hércia Stampini Duarte Martino","doi":"10.1016/j.cnd.2026.08.040","DOIUrl":"10.1016/j.cnd.2026.08.040","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the bioactive potential of soursop (<em>Annona muricata</em> L.) by-products (peel, pomace and seeds) and their effects on toxicity and oxidative stress in <em>Caenorhabditis elegans</em>.</div></div><div>To evaluate the bioactive potential of soursop (<em>Annona muricata</em> L.) by-products (peel, pomace and seeds) and their effects on toxicity and oxidative stress in <em>Caenorhabditis elegans</em>.</div><div><h3>Methods</h3><div>The three fractions were freeze-dried, ground, and extracted with 50% ethanol. Total phenolics, flavonoids, condensed tannins, and antioxidant capacity (DPPH, ABTS) were measured. <em>C. elegans</em> N2 (L4 stage) were exposed to 5%, 0.5%, and 0.1% (w/v) by-products for toxicity; survival was assessed by movement. For oxidative stress, nematodes were co-exposed to 0.5% extracts and 4–12<!--> <!-->mM H<sub>2</sub>O<sub>2</sub> for 24<!--> <!-->h. Antioxidant properties were analyzed using one-way ANOVA followed by Tukey's. <em>C. elegans</em> assays were analyzed using Student's t-test against the control group.</div></div><div>The three fractions were freeze-dried, ground, and extracted with 50% ethanol. Total phenolics, flavonoids, condensed tannins, and antioxidant capacity (DPPH, ABTS) were measured. <em>C. elegans</em> N2 (L4 stage) were exposed to 5%, 0.5%, and 0.1% (w/v) by-products for toxicity; survival was assessed by movement. For oxidative stress, nematodes were co-exposed to 0.5% extracts and 4–12<!--> <!-->mM H<sub>2</sub>O<sub>2</sub> for 24<!--> <!-->h. Antioxidant properties were analyzed using one-way ANOVA followed by Tukey's. <em>C. elegans</em> assays were analyzed using Student's t-test against the control group.</div><div><h3>Results</h3><div>Peel exhibited the highest antioxidant capacity (μmol TE/g), reaching 10,615.08<!--> <!-->±<!--> <!-->363.50 (DPPH) and 163.3<!--> <!-->±<!--> <!-->4.8 (ABTS), followed by the pomace (2078.21<!--> <!-->±<!--> <!-->35.76 and 62.1<!--> <!-->±<!--> <!-->1.6), seed exhibited the lowest activity (183.43<!--> <!-->±<!--> <!-->1.43 and 1.2<!--> <!-->±<!--> <!-->0.0). Peel presented the highest levels of total phenolic compounds (2658.16<!--> <!-->±<!--> <!-->120.65 mgGAE/100<!--> <!-->g), flavonoids (252.65<!--> <!-->±<!--> <!-->8.7 mgEC/100<!--> <!-->g) and tannins (100.7<!--> <!-->±<!--> <!-->4.9 mgEC/g), followed by pomace (908.53<!--> <!-->±<!--> <!-->36.04 mgGAE/100<!--> <!-->g, 172.78<!--> <!-->±<!--> <!-->25 mgEC/100<!--> <!-->g and 64.2<!--> <!-->±<!--> <!-->2.8 mgEC/g, respectively). Seeds had the lowest values (173.13<!--> <!-->±<!--> <!-->3.58 mgGAE/100<!--> <!-->g, 21.23<!--> <!-->±<!--> <!-->0.4 mgEC/100<!--> <!-->g and 1.07<!--> <!-->±<!--> <!-->0.6 mgEC/g). In <em>C. elegans</em>, peel and pomace showed no toxicity at 5%, 0.5%, and 0.1%. The seed reduced survival at 5%, but not at 0.5% or 0.1%. Under oxidative stress, the seed enhanced <em>C. elegans</em> survival at 4, 8, and 12<!--> <!-->mM H<sub>2</sub>O<sub","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Pages e22-e23"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early-life kefir intake reduces disease activity and colonic histopathological damage in wistar rats with induced colitis","authors":"Poliana Guiomar de Almeida Brasiel, Ana Clara Fernandes Peixoto, Eduardo Nascimento Barreto, Thiago Alves da Silva, Ana Carolina Oliveira Simões, Gabriela de Cássia Ávila Alpino, Ricardo Reis Rodrigues, Érika de Fátima dos Santos, Eliana Cristina de Brito Toscano, Sheila Cristina Potente Dutra Luquetti","doi":"10.1016/j.cnd.2026.08.051","DOIUrl":"10.1016/j.cnd.2026.08.051","url":null,"abstract":"<div><h3>Introduction</h3><div>Inflammatory bowel diseases (IBD), are the result of multifactorial processes involving genetic, immune, microbiota, and environmental factors. Their incidence is increasing, especially in developing countries such as Brazil. Early-life microbiota manipulation has shown promise in preventing IBD. Therefore, probiotics may play a role in this process. The present study explores the potential impact of kefir and <em>Limosilactobacillus reuteri</em> DSM 17938 (<em>L. reuteri</em>) on IBD susceptibility and aims to guide the development of preventative strategies.</div></div><div>Inflammatory bowel diseases (IBD), are the result of multifactorial processes involving genetic, immune, microbiota, and environmental factors. Their incidence is increasing, especially in developing countries such as Brazil. Early-life microbiota manipulation has shown promise in preventing IBD. Therefore, probiotics may play a role in this process. The present study explores the potential impact of kefir and <em>Limosilactobacillus reuteri</em> DSM 17938 (<em>L. reuteri</em>) on IBD susceptibility and aims to guide the development of preventative strategies.</div><div><h3>Methods</h3><div>Lactation Wistar rats were divided: Control (C); Kefir control (CK); <em>L. reuteri</em> control (CL); Overfeeding (S); Kefir overfeeding (SK); and <em>L. reuteri</em> overfeeding (SL). The C and S rats received 1<!--> <!-->mL of water/day; Kefir and <em>L. reuteri</em> groups received 1<!--> <!-->mL of probiotics (109 colony forming unit, cfu/day) during lactation. After weaning, the pups continued to receive the same treatment until 30 days. At 140 days of age, the animals were induced to colitis by 3% (w/v) dextran sulfate sodium (DSS) in drinking water for 7 days. Body weight, disease activity index (DAI) score and histopathological evaluation were measured. The experimental protocol was approved by the Ethics Committee for the use of Animals at the Federal University of Juiz de Fora (n.09/2025).</div></div><div>Lactation Wistar rats were divided: Control (C); Kefir control (CK); <em>L. reuteri</em> control (CL); Overfeeding (S); Kefir overfeeding (SK); and <em>L. reuteri</em> overfeeding (SL). The C and S rats received 1<!--> <!-->mL of water/day; Kefir and <em>L. reuteri</em> groups received 1<!--> <!-->mL of probiotics (109 colony forming unit, cfu/day) during lactation. After weaning, the pups continued to receive the same treatment until 30 days. At 140 days of age, the animals were induced to colitis by 3% (w/v) dextran sulfate sodium (DSS) in drinking water for 7 days. Body weight, disease activity index (DAI) score and histopathological evaluation were measured. The experimental protocol was approved by the Ethics Committee for the use of Animals at the Federal University of Juiz de Fora (n.09/2025).</div><div><h3>Results</h3><div>Offspring overfed during lactation exhibited higher body weight at the end of the lactation period than the control g","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e29"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between the FTO rs9939609 polymorphism and energy, macronutrient, and fiber intake in adults with obesity: Preliminary results of a systematic review of observational studies","authors":"Matheus Maia Soares, Hilana Moreira Paiva, Sandy Maira Almeida de Andrade, Alessandra Silva, Flavia Lucia Conceição, Fernanda Cristina C. Mattos, João Regis Ivar Carneiro","doi":"10.1016/j.cnd.2026.08.079","DOIUrl":"10.1016/j.cnd.2026.08.079","url":null,"abstract":"<div><h3>Objective</h3><div>The influence of the FTO rs9939609 polymorphism on dietary intake in adults with obesity remains poorly understood. Thus, the objective of this study is to systematically review the association between the FTO rs9939609 polymorphism and total energy, macronutrient (fat, carbohydrate, and protein), and fiber intake in adults with obesity.</div></div><div>The influence of the FTO rs9939609 polymorphism on dietary intake in adults with obesity remains poorly understood. Thus, the objective of this study is to systematically review the association between the FTO rs9939609 polymorphism and total energy, macronutrient (fat, carbohydrate, and protein), and fiber intake in adults with obesity.</div><div><h3>Methods</h3><div>This systematic review was conducted in accordance with PRISMA 2020 guidelines and registered with PROSPERO (CRD420261344866). Electronic searches were performed in MEDLINE, EMBASE, and Scopus for studies published between 2015 and 2026, supplemented by manual screening of reference lists. Eligibility criteria followed the PECOS framework: adults with obesity (BMI<!--> <!-->≥<!--> <!-->30<!--> <!-->kg/m<sup>2</sup>); FTO rs9939609 genotyping as exposure; comparison between risk allele carriers (AA/AT) versus non-carriers (TT); outcomes included total energy, macronutrient, micronutrient, and fiber intake; observational study designs (cross-sectional, cohort, or case-control). Studies in children or animals and those lacking extractable data were excluded. Titles and abstracts were screened independently by three reviewers, with discrepancies resolved by consensus. The Rayyan software was used for duplicate management.</div></div><div>This systematic review was conducted in accordance with PRISMA 2020 guidelines and registered with PROSPERO (CRD420261344866). Electronic searches were performed in MEDLINE, EMBASE, and Scopus for studies published between 2015 and 2026, supplemented by manual screening of reference lists. Eligibility criteria followed the PECOS framework: adults with obesity (BMI<!--> <!-->≥<!--> <!-->30<!--> <!-->kg/m<sup>2</sup>); FTO rs9939609 genotyping as exposure; comparison between risk allele carriers (AA/AT) versus non-carriers (TT); outcomes included total energy, macronutrient, micronutrient, and fiber intake; observational study designs (cross-sectional, cohort, or case-control). Studies in children or animals and those lacking extractable data were excluded. Titles and abstracts were screened independently by three reviewers, with discrepancies resolved by consensus. The Rayyan software was used for duplicate management.</div><div><h3>Results</h3><div>A total of 1,084 articles were identified, of which 421 duplicates were removed, resulting in 663 articles screened by title and abstract. Of these, 20 articles were read in full, and 3 met the eligibility criteria and were included. All included studies are cross-sectional; two were conducted in Brazil and one in Norway. A total of","PeriodicalId":43722,"journal":{"name":"CAHIERS DE NUTRITION ET DE DIETETIQUE","volume":"61 ","pages":"Page e43"},"PeriodicalIF":0.3,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149028303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}