Talanta Open最新文献

{"title":"3D printed hardware for automation of proteomics sample preparation at the Meso-Scale","authors":"Sadie R. Schultz ,&nbsp;Matthew M. Champion","doi":"10.1016/j.talo.2025.100505","DOIUrl":"10.1016/j.talo.2025.100505","url":null,"abstract":"<div><div>Mass spectrometry-based proteomics is the dominant method for measuring peptides and proteins from complex mixtures. In bottom-up approaches, proteins are digested or proteolyzed prior to LC-MS/MS analysis. Peptides are fragmented, and proteins are inferred <em>via</em> peptide spectral matching (PSM). The throughput of this process is surprisingly low; a proteomics core facility might analyse &lt;20 samples/day per instrument using UHPLC-MS/MS. Because of this, automation in proteomics is rare, and virtually all preparation is performed by hand. We developed 3D printed hardware and automated sample preparation modules for a lower-cost Andrew Alliance pipetting robot. The robot operates on simple principles, using traditional pipettes and follows protocols closely resembling manual preparation. Here, we present modular protocols for the major techniques in proteomics preparation: in-solution and S-Trap digestion; Tip and solid-phase extraction (SPE) based desalting. Both approaches yield dense protein identifications from complex proteomes. Automated samples had high reproducibility: ∼60 % of proteins identified from in-solution and S-Trap digested samples had a measured CV of ≤20 %. In contrast, 52 % of in-solution digested and 63 % of S-Trap digested of proteins identified from manually prepared samples had CVs ≤20 %. Automated sample digestion and tip-based desalting had reduced ≅ 70 % and 40 % quantitative yield respectively compared to manual preparation according to the protein label-free quantification (LFQ). Increasing injection amount to normalize the yield restored protein and peptide identifications which demonstrates the differences between manual and automated methods were predominantly due to reduced recovery. Overall, automation of bottom-up proteomics sample preparation at the meso‑scale offers increased reproducibility in non sample-limited applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100505"},"PeriodicalIF":4.1,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of atmospheric pressure DBD plasma treatment on the biodegradability, hydrophilicity, and mechanical properties of PVA/PEG/Chitosan/HA nanofibers for bone tissue engineering","authors":"Hartatiek ,&nbsp;M.I. Wuriantika ,&nbsp;Yudyanto ,&nbsp;A. Taufiq ,&nbsp;M. Diantoro ,&nbsp;Y. Yusuf ,&nbsp;M. Taufik ,&nbsp;J.F. Fatriansyah","doi":"10.1016/j.talo.2025.100501","DOIUrl":"10.1016/j.talo.2025.100501","url":null,"abstract":"<div><div>The effectiveness of scaffolds in supporting bone tissue regeneration is highly dependent on surface modification. Atmospheric pressure dielectric barrier (DBD) plasma treatment offers a practical method that offers simplicity, time efficiency, cost effectiveness, and reliable results. In this study, nanofiber scaffolds composed of PVA, PEG, chitosan, and hydroxyapatite were fabricated using electrospinning and treated with DBD plasma at varying distances. Optical emission spectroscopy (OES) confirmed the presence of reactive species such as OH radicals, nitrogen (N₂), and nitrogen ions (N₂⁺). These species caused changes in surface morphology, including an increase in fiber diameter. These surface morphological modifications were associated with mechanical properties and hydrophilicity. Furthermore, scaffolds treated at a distance of 1.5 mm showed a degradation rate of up to 70 % after three weeks. The results highlight the potential of DBD plasma treatment to optimize the performance of bone tissue engineering scaffolds.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100501"},"PeriodicalIF":4.1,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural isomeric aromatic diamine linkers influenced electrocatalysis of AuNPs: Efficient assessment of hydrazine in environmental water samples","authors":"Karmegam Muthukrishnan ,&nbsp;N.S.K. Gowthaman ,&nbsp;Bharathi Sinduja ,&nbsp;Mathur Gopalakrishnan Sethuraman","doi":"10.1016/j.talo.2025.100503","DOIUrl":"10.1016/j.talo.2025.100503","url":null,"abstract":"<div><div>This electrochemical research explores the conductive behaviour and sensor functionality of citrate-stabilized gold nanoparticles (AuNPs) constructed on a glassy carbon (GC) electrode. Aromatic diamines (ArDAs) with different structural isomeric forms were used as efficient linkers for the attachment of AuNPs, with particular attention given to the detection of hydrazine (HYZ). Careful analysis of three distinct ArDAs, ortho-phenylenediamine (OPD), meta-phenylenediamine (MPD), and para-phenylenediamine (PPD), revealed the formation of self-assembled monolayers (SAMs) of ArDAs on the GC electrode surface. The growth kinetics of ArDA SAMs on GC surfaces were examined using ATR-FT-IR, focusing on the -NH₂ bending vibration region (1592–1631 cm^-1), and the Langmuir adsorption kinetics described the SAM formation, specifically, faster and easier formation of PPD-SAM than OPD and MPD. The PPD SAM on the electrode surface was found to be more compact than OPD and MPD, according to the multi-method analysis. The synthesized AuNPs were anchored on the electrode surface through the free amine groups present in the SAMs and confirmed through scanning electron microscopy, X-ray photoelectron spectroscopy, and cyclic voltammetry. The AuNPs/PPD/GC electrode exhibited superior sensitivity (562 µA mM^-1 cm^-2) and a low detection limit (30 nM) towards HYZ determination. Further, the practicability of the proposed sensor is evaluated by detecting HYZ in environmental water samples and validating the results with the ultra-high-performance liquid chromatography method.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100503"},"PeriodicalIF":4.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic and quantitative lateral flow assay for grayscale imaging of neutralizing antibodies SARS-CoV-2","authors":"Karla R. Castro ,&nbsp;Isabela A. Mattioli ,&nbsp;Graziela C. Sedenho ,&nbsp;Thiago Bertaglia ,&nbsp;Antônio F.A. Araújo ,&nbsp;Manoel J.A. Lima ,&nbsp;Beatriz G.R. da Silva ,&nbsp;Mona N. Oliveira ,&nbsp;Iris Todeschini ,&nbsp;Phelipe M. Vitale ,&nbsp;Erika R. Manuli ,&nbsp;Geovana M. Pereira ,&nbsp;Suzete C. Ferreira ,&nbsp;Ester C. Sabino ,&nbsp;Emanuel Carrilho ,&nbsp;Frank N. Crespilho","doi":"10.1016/j.talo.2025.100504","DOIUrl":"10.1016/j.talo.2025.100504","url":null,"abstract":"<div><div>Lateral flow immunoassays (LFIAs) have modernized decentralized mass testing and significantly impacted the healthcare system. It provides a rapid and accessible method for serological testing, which is an important tool for screening patients with past viral phases by detecting antibodies. Here, the development of a quantitative LFIA is presented, in which the receptor-binding domain of Spike protein-specific antibody (anti-S-RBD) in serum samples from previously infected with SARS-CoV-2 patients form an immune complex with the RBD protein immobilized on gold nanoparticles. For quantitative measurements, a methodology was developed based on the plasmonic behavior of gold nanoparticles and using a digital camera that processes images of the test lines in a closed chamber using the CYMK color system. The limits of detection and quantification were 0.22 and 0.70 µg mL^–1, respectively. The proposed device has a manufacturing cost of less than US$ 1 from synthesizing the bioconjugates, assembly of the LFIA strips, and 3D printing of the cassettes. The platform could detect anti-S-RBD antibodies in human serum samples and can be particularly useful in strategies for monitoring IgG levels in the population.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100504"},"PeriodicalIF":4.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free masterful spectrofluorimetric systems for minuscule tracking of natural antioxidant vincamine in the pharmaceutical, environmental, and biological samples: Comparative greenness, whiteness, and blueness appraisal","authors":"Eman Yosrey ,&nbsp;Heba Elmansi ,&nbsp;Shereen Shalan ,&nbsp;Jenny Jeehan Nasr","doi":"10.1016/j.talo.2025.100502","DOIUrl":"10.1016/j.talo.2025.100502","url":null,"abstract":"<div><div>In this work, we present two label-free spectrofluorimetric systems for the swift quantification of vincamine (VN) in various matrices without the need for knotty fluorescent dyes, drastic conditions, or expensive instrumentation, which are key merits of the quantifying procedures. The first method (Method I) relied on the direct mix-and-read assay for exploiting the intrinsic fluorescence of VN, adopting λ_ex/em of 272/334 nm using water as a diluting solvent. The method fitted linearity across the concentrations of 1.00 – 10.00 µg mL⁻¹. In the second method (Method II), upon turning off the photoinduced electron transfer (PET) of tertiary amine in the piperidine moiety to the indole scaffold using 0.1 M hydrochloric acid, the fluorescent signal was augmented up to 158 %. This commendable boost in the fluorescent sensitivity allowed the minute detection of VN in spiked human plasma with a standard deviation not exceeding 1.30. Using the acid-blocked PET-based probe, the fluorescent signal was linearly correlated with VN concentration across 0.25 – 6.00 µg mL⁻¹ using λ_ex/em of 277/323 nm. These streamlined procedures proficiently evaluated VN in both the sole and co-formulated capsules without any significant interference from capsule filler material or the co-formulated medication “piracetam”. In addition, the two submitted procedures were successfully executed to check VN through weight variation testing in the Brain-ox® capsules with good acceptance values. The constructed platforms ensemble estimated VN in the environmental samples (tap and river water) with acceptable percentage recoveries and standard deviations (0.95 – 1.65). The procedures' green, white, and blue fitness were appraised through a plethora of computational tools, including GAPI, AGREE, AGREEprep, the RGB 12 algorithm, and BAGI. The evaluation showed that the proposed works effectively strike a compromise between sustainability and functionality postulates. This highlighted that designed approaches are perfectly suited as ecological frameworks for minuscule assaying of VN in crucial pharmaceutical, biological, and environmental samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100502"},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144338779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly selective room temperature detection of NO2 enabled by vanadyl oxygen vacancies in novel bilayer V2O5","authors":"Reshma P R,&nbsp;Arun K Prasad","doi":"10.1016/j.talo.2025.100497","DOIUrl":"10.1016/j.talo.2025.100497","url":null,"abstract":"<div><div>The primary challenge in developing a gas sensor is achieving high selectivity for the target gas. Most sensor materials respond to multiple gases, making it difficult to discern between various toxic gases. The present study reports the enhancement of the selectivity towards NO₂ gas by introducing vanadyl oxygen (O_I) vacancies in novel 2D V₂O₅. The chemical exfoliation process, which is utilized in the present study to synthesize bilayer nanosheets of V₂O₅, intrinsically generates O_I vacancies. The presence of O-vacancy defects, predominantly O_I vacancies, in the sample is confirmed using X-ray photoelectron spectroscopy, photoluminescence spectroscopy, and Raman spectroscopy. The bilayer 2D V₂O₅ showed a highly selective chemiresistive response towards NO₂ gas at room temperature unlike normally observed higher temperature sensor response by V₂O₅, typically above 100 °C. Along with the effect of high surface to volume ratio, the room temperature gas sensing performance by 2D V₂O₅ stems from the presence of O_I vacancy defects and the consequent increase in the surface activity. In addition, the presence of O_I vacancies leads to highly selective response to NO₂, since NO₂ is a highly oxidizing gas with a pair of lone electrons. Hence, the present study is the first to reveal novel bilayer V₂O₅ sensor with a highly selective response to NO₂ at ambient temperature.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100497"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrinsic fluorescence in Moringa Oleifera seed extract","authors":"Namrah Azmi ,&nbsp;Sachin Rathod ,&nbsp;Bruno Telli Ceccato ,&nbsp;Rasmus Kranold Mikkelsen ,&nbsp;Jon Otto Fossum","doi":"10.1016/j.talo.2025.100500","DOIUrl":"10.1016/j.talo.2025.100500","url":null,"abstract":"<div><div>Autofluorescence, an intrinsic property of biological molecules that emit light upon excitation, is gaining significance as a noninvasive technique for studying plant-derived bioactive compounds. This phenomenon enables detection of biomolecules such as chlorophyll, lignin, flavonoids, and alkaloids without requiring external dyes or destructive extraction processes. Intrinsic fluorescence can also aid in detecting and identifying biomolecules that typically require extensive sample preparation (molecule labelling with external fluorophores), eliminating the need for external probes. The present study aims to report the intrinsic autofluorescence in the <em>Moringa Oleifera</em> plant, highlighting its potential to enhance noninvasive bioactive compound identification and quality assessment in plant-based products.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100500"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advanced analytical and digital approaches for proactive detection of food fraud as an emerging contaminant threat","authors":"Ahmed S. El-tahlawy ,&nbsp;Abdullah S. Alawam ,&nbsp;Hassan A. Rudayn ,&nbsp;Ahmed A. Allam ,&nbsp;Rehab Mahmoud ,&nbsp;Hany Abd El-Raheem ,&nbsp;Waleed Alahmad","doi":"10.1016/j.talo.2025.100499","DOIUrl":"10.1016/j.talo.2025.100499","url":null,"abstract":"<div><div>The integrity of the global food supply is increasingly compromised by sophisticated fraudulent methods such as adulteration, mislabeling, and substitution. These deceptive practices introduce undisclosed and harmful substances that often evade traditional detection, posing significant risks to public health and consumer trust. This review synthesizes cutting-edge advancements in analytical and digital technologies that are redefining food fraud surveillance. We explore the convergence of molecular diagnostics (e.g., DNA barcoding, clustered regularly interspaced short palindromic repeats (CRISPR), next-generation sequencing (NGS)), high-throughput spectroscopic platforms (e.g., Raman spectroscopy, mass spectrometry), biosensors, and digital innovations (e.g., artificial intelligence (AI), Internet of Things (IoT), blockchain, and lab-on-a-chip (LOC) devices). These integrated approaches enable real-time, proactive detection of fraud across complex and globalized supply chains, offering sensitivity, scalability, and traceability previously unattainable. By framing food fraud as a form of emerging contamination, this work aligns with the paradigm shift toward predictive, data-driven risk assessment in contaminant detection. The review also addresses key translational bottlenecks such as cost, standardization, and interoperability, and proposes a systems-level roadmap to bridge scientific innovation with regulatory and industrial application. This interdisciplinary perspective advocates for a future where food authenticity assurance is intelligent, holistic, and resilient in the face of evolving adulteration threats.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100499"},"PeriodicalIF":4.1,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of low-dimensional MoS2 as a smart nanoplatform for environmental technologies, therapeutic strategies, and biomedical sensing","authors":"Smriti Gaba,&nbsp;Mridul Sahu,&nbsp;Nidhi Chauhan,&nbsp;Utkarsh Jain","doi":"10.1016/j.talo.2025.100498","DOIUrl":"10.1016/j.talo.2025.100498","url":null,"abstract":"<div><div>As one of the representative layered transition metal dichalcogenides (LTMDC), molybdenum disulfide (MoS₂) has been widely researched as a promising nanoplatform for biomedicine as well as green technologies. The features of high surface-to-volume ratio, tunable bandgap, and good biocompatibility make it a promising candidate for therapeutic strategies, biomedical detection, and environmental applications. Low-dimensional MoS₂ has been widely investigated for drug delivery, bioimaging, photothermal therapy (PTT), photodynamic therapy (PDT), biosensing, and environmental innovations in the past few years, owing to its strong interaction with biomolecules and the cellular microenvironment. The utility of MoS₂ as an alternative to traditional nanomaterials has been assisted by various functionalization strategies to improve solubility, stability, and targeted applications. Moreover, MoS₂-based biosensors have demonstrated remarkable sensitivity for detecting biomolecules, pathogens, and disease-specific biomarkers that enable early and accurate disease diagnosis. This ability is critical to precision medicine, which allows for personalized treatment approaches, real-time health monitoring, and target activation or suppression of pathways based on a patient's biological profile. Additionally, it has become a beacon of environmental application innovation, providing long-term answers to urgent ecological problems. Because of special physicochemical characteristics, contaminants, including gases, organic compounds, heavy metals, and radionuclides, can be effectively removed from the environment, leading to cleaner air and water supplies. Nonetheless, industrial translation of MoS₂ requires the resolution of toxicity, long-term stability, and large-scale synthesis issues. This review aims to comprehensively discuss the recent development, biomedical and environmental applications, challenges, and future perspectives of low-dimensional MoS₂ in the field of next-generation sustainable technology.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100498"},"PeriodicalIF":4.1,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DART-MS approaches for metabolomics-based authentication: Challenges in determining the geographical origin of strawberries (Fragaria × ananassa)","authors":"Felix Schmauder,&nbsp;Johannes Brockelt,&nbsp;Kim Brettschneider,&nbsp;Stephan Seifert,&nbsp;Marina Creydt,&nbsp;Markus Fischer","doi":"10.1016/j.talo.2025.100496","DOIUrl":"10.1016/j.talo.2025.100496","url":null,"abstract":"<div><div>Metabolomics-based approaches are a widely used method for determining the origin of food. The aim of this study was to investigate the suitability of metabolomics analysis using direct analysis in real time mass spectrometry (DART-MS) to ascertain the origin of strawberries. In addition, optimization possibilities and limitations were identified by testing different extraction methods, two different sample delivery systems (Quick-Strip and Dip-it approaches), and running some of the measurements in both positive and negative ionization mode proved to be the most suitable. A total of 207 strawberry samples from six countries were measured and classified using random forest with this optimized approach. The classification accuracy was 63.8%, which shows certain limitations of the approach presented. However, another aim of this study was to protect German strawberries from adulteration with foreign strawberries, and this was achieved with a promising accuracy of 90.3%.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100496"},"PeriodicalIF":4.1,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144307333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}