Talanta Open最新文献

{"title":"Transforming a traditional ethnic beverage into highly emissive CDs as a sensitive nanosensor for the anti-malignant hyperthermia drug dantrolene sodium in pharmaceuticals and plasma. An economic and sustainable approach","authors":"Samar Salem,&nbsp;Rania El-Shaheny,&nbsp;Nora A. Abdallah","doi":"10.1016/j.talo.2025.100549","DOIUrl":"10.1016/j.talo.2025.100549","url":null,"abstract":"<div><div>This work provides a cost-effective, environmentally benign, rapidly prepared, highly fluorescent carbon dots (CDs) using renewable antioxidants-rich feedstock (<em>Tamarindus Indica</em> L.) as the precursor. A home-available and less energy-consuming microwave oven synthesizes CDs within 5 min. The created CDs have the highest fluorescence intensity at 402 nm after being excited at 328 nm, conferring a high luminescence quantum yield of 33.5 %. The prepared CDs provide ultra-sensitive and selective determination of dantrolene sodium (DAN), the only synthetic drug that can manage malignant hyperthermia up to now, and of extraordinary antioxidant properties. The determination is over a wide concentration range of (1.25–100.0 µM) with a limit of detection (LOD) and limit of quantification (LOQ) of 0.07 µM and 0.21 µM, respectively. DAN was estimated in two different pharmaceutical dosage forms, yielding percentage recoveries of 99.49 ± 1.50 and 99.74 ± 0.97. The proposed method was also successfully applied for the assay of DAN in spiked human plasma with a % recovery of 99.55 ± 3.13. Various greenness metrics were used to elucidate the greenness of the nanoprobe. The greenness of this work stems from the fabrication of CDs from natural, widespread, cheap, and edible fruit using a less energy and time-consuming, easily operated, and safe reactor. Moreover, avoiding harsh chemicals and solvents and using distilled water as the diluent renders the method greener. The above-mentioned facts about the proposed method prove it to be a validated analytical approach for different pharmaceutical and biological applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100549"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thorium determination in seawater by inductively coupled plasma mass spectrometry: Method development and oceanographic applications","authors":"Yingjie Zou ,&nbsp;Peng Wang ,&nbsp;Lei Xu ,&nbsp;Hong Ren ,&nbsp;Yiyao Cao","doi":"10.1016/j.talo.2025.100553","DOIUrl":"10.1016/j.talo.2025.100553","url":null,"abstract":"<div><div>Thorium isotopes in the ocean (including radiogenic isotopes and terrigenous-derived ²³²Th) serve as key tracers for studying land-derived material transport. While high-sensitivity and high-resolution inductively coupled plasma mass spectrometry (ICP-MS) has become the mainstream technique for seawater thorium isotope analysis, its application faces three major challenges: complex pretreatment purification procedures, varying sample volume requirements, and significant instrument memory effects. This review systematically summarizes the technical difficulties and solutions in ICP-MS-based thorium isotope detection and examines its oceanographic applications in quantifying dust inputs and tracing water mass mixing, thereby providing critical data for assessing marine carbon pump efficiency.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100553"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed carbon black-based electrode for simultaneous determination of ascorbic acid and kojic acid in dermo-cosmetic products","authors":"Larissa Gil Lucas ,&nbsp;Eduardo Constante Martins ,&nbsp;Pãmyla Layene dos Santos ,&nbsp;João Paulo Winiarski ,&nbsp;Edson Roberto Santana ,&nbsp;Iolanda Cruz Vieira ,&nbsp;Almir Spinelli","doi":"10.1016/j.talo.2025.100532","DOIUrl":"10.1016/j.talo.2025.100532","url":null,"abstract":"<div><div>A miniaturized and disposable poly(lactic acid)-carbon black-based working electrode (3D CB-PLA) was developed using 3D printing technology through fused filament fabrication to be applied to the simultaneous analysis of the antioxidant compounds ascorbic acid (vitamin C) and kojic acid. A previous chemical/electrochemical activation of the 3D CB-PLA electrode was carried out in 1.0 mol L⁻¹ NaOH, applying a fixed potential of +1.0 V <em>vs</em>. Ag/AgCl, KCl_(sat) for 1800 s. This procedure was necessary to remove a smooth layer of PLA polymer on the electrode surface and to allow the carbon black conductive sites to be exposed. Morphological and spectroscopic characterizations performed before and after activation revealed a rougher surface with an increased presence of oxygenated functional groups, attributed to both the activation process and the enhanced exposure of carbon black. Using cyclic voltammetry in 0.1 mol L⁻¹ Britton-Robinson buffer (pH 7.0), the irreversible oxidation reactions of ascorbic and kojic acids were observed at +0.30 and +0.87 V <em>vs</em>. Ag/AgCl, KCl_(sat), respectively. With the experimental conditions optimized, calibration curves were constructed for both analytes by square wave voltammetry, with detection limits of 4.80 and 5.40 µmol L⁻¹ for ascorbic acid and kojic acid, respectively. The activated 3D CB-PLA electrode enabled accurate and precise simultaneous quantification of both antioxidant compounds in facial whitening creams, demonstrating its effectiveness and analytical potential.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100532"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput microfluidic generation of self-assembled, uniform 3D vascularized and mineralized bone organoids without matrix biomaterials","authors":"Xue Li ,&nbsp;Zijiao Zhang ,&nbsp;Tian Tian ,&nbsp;Chen Chen ,&nbsp;Xin Xu ,&nbsp;Yuzhu He ,&nbsp;Yaran Zang ,&nbsp;Jianan Hui ,&nbsp;Hongju Mao ,&nbsp;Huiying Liu","doi":"10.1016/j.talo.2025.100531","DOIUrl":"10.1016/j.talo.2025.100531","url":null,"abstract":"<div><div>Bone organoids hold great promise for modeling bone-related diseases, improving bone injury repair strategies, and enabling high-throughput drug screening. However, conventional approaches rely heavily on matrix biomaterials—such as Matrigel, collagen gels, or 3D-printed scaffolds—which introduce undefined parameters, pose manufacturing complexities, and raise cost barriers, potentially limiting clinical translation. To address these challenges, we present a novel high-throughput microfluidic chip that generates 540 uniformly sized, scaffold-free 3D bone organoids simultaneously within six independent channels. By co-cultivating human bone marrow mesenchymal stem cells (hBMSCs), human umbilical vein endothelial cells (HUVECs), and mineralized collagen (MC), self-assembled bone organoids formed by the third day and progressively compacted over time, exhibiting enhanced cell viability and proliferation. The inclusion of MC upregulated multiple osteogenic markers (OCN, ALP, COL-1, RUNX2, and BMP-2), while endothelial markers (PECAM-1, HIF-1α, and VEGF) remained consistently expressed, reflecting stable vascularization and mineralization potential. Overall, this high-throughput, matrix-free microfluidic platform offers a biomimetic environment for the investigation of osteogenesis and angiogenesis and holds significant promise for advanced material assessment, drug screening, and disease modeling.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100531"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144904572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanozyme activity of silver nanowires for colorimetric detection of bisphenol A following salting-out assisted liquid-liquid extraction","authors":"Huda Salem AlSalem ,&nbsp;Sara Naif Alharbi ,&nbsp;Mohamed A Abdel-Lateef ,&nbsp;Rabeea D. Abdel-Rahim ,&nbsp;Wesam M. El-Koussi","doi":"10.1016/j.talo.2025.100587","DOIUrl":"10.1016/j.talo.2025.100587","url":null,"abstract":"<div><div>The increasing use of industrial chemicals has raised major concerns about their environmental and health impacts, with bisphenol A (BPA) being one of the most widely studied contaminants. In this work, we introduce a proof-of-concept approach that combines the peroxidase-like catalytic activity of silver nanowires (AgNWs) with a salting-out assisted liquid–liquid extraction (SALLE) strategy for BPA detection. AgNWs catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in an acidic environment, producing a measurable color change that is effectively suppressed in the presence of BPA. This inhibition enables a simple colorimetric quantification of BPA with a detection limit of 31.62 ng/mL (LOQ = 95.83 ng/mL). The method demonstrated a practical linear response within the range of 200–700 ng/mL, making it suitable for screening moderately contaminated water samples. Furthermore, the work offers good recovery values for the extraction of BPA from spiked environmental water samples. Integrating AgNWs nanoenzyme activity with an eco-friendly pre-concentration step provides a promising low-cost, straightforward, and rapid alternative to conventional chromatographic methods, particularly suited for preliminary screening of contaminated waters.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100587"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145463088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphene oxide and molecularly imprinted polymer-based sensor for the electrochemical detection of benzene: A novel tactic in early diagnosis of lung cancer","authors":"Warren Rosario ,&nbsp;Devesh K. Avasthi ,&nbsp;Nidhi Chauhan","doi":"10.1016/j.talo.2025.100506","DOIUrl":"10.1016/j.talo.2025.100506","url":null,"abstract":"<div><div>Early cancer detection significantly enhances survival rates, making widespread diagnostic screening essential. However, the high cost and limited accessibility of diagnostic tools pose major challenges. Nanomaterial-based sensors offer a promising solution due to their portability, user-friendly design, and cost-effective manufacturing. Volatile organic compounds (VOCs) emitted by the human body are closely linked to the presence of life-threatening diseases, with benzene emerging as a significant biomarker in the breath and blood of individuals at risk of developing lung cancer. Leveraging this correlation, we have developed a sensor capable of detecting benzene with high reliability. This study details the fabrication of an electrochemical sensor on a screen-printed electrode (SPE), modified with graphene oxide (GO) followed by molecularly imprinted polymer (MIP). SPE promotes the development of a miniaturized and user-friendly sensing platform. The incorporation of GO is responsible for improving the sensor's response due to its excellent electrochemical properties. The MIP is synthesized using benzene as a template, which creates imprints of benzene on the polymer. Integration of this MIP significantly improves the sensor’s selectivity. This approach addresses the key limitation of low selectivity in VOC sensors for medical applications. The sensor demonstrated a broad linear range of 0.1–1000 ppb with a sensitivity of 15.5 µA/ppb and exhibited excellent selectivity toward benzene. By enhancing both sensitivity and selectivity, the developed sensor offers a promising solution toward effective, accessible, and early lung cancer diagnosis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100506"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144549660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on biosensor approaches for the detection of hazardous elements in water","authors":"Nidhi Chauhan ,&nbsp;Nishtha Sah ,&nbsp;Kirti Saxena ,&nbsp;Utkarsh Jain","doi":"10.1016/j.talo.2025.100536","DOIUrl":"10.1016/j.talo.2025.100536","url":null,"abstract":"<div><div>Water is the basis of life on Earth, but due to increased pollution and habitat degradation, water scarcity is increasing day by day. Therefore, it is necessary to detect water pollutants to save water and aquatic diversity. Monitoring of toxic elements present in wastewater effluents is an important factor for further treatment to make it pollutant-free. While various methods have been developed for monitoring these toxic elements, biosensors can overcome the limitations of traditional methods. Biosensors are highly sensitive, selective, low in cost, and simple to use. In this review, we have provided an overview of reported biosensors for the detection of various types of toxic elements that cause water toxicity. A summary of these toxic elements has also been discussed herein. This review aims to provide an overview of advances in biosensing technology used for water monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100536"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wearable biosensors in modern healthcare: Emerging trends and practical applications","authors":"Maumita Das Mukherjee ,&nbsp;Priyanshi Gupta ,&nbsp;Vanshika Kumari ,&nbsp;Ishika Rana ,&nbsp;Drishti Jindal ,&nbsp;Navya Sagar ,&nbsp;Jay Singh ,&nbsp;Chetna Dhand","doi":"10.1016/j.talo.2025.100486","DOIUrl":"10.1016/j.talo.2025.100486","url":null,"abstract":"<div><div>Wearable biosensor systems represent a significant breakthrough in the life sciences, offering real-time monitoring and quantitative assessment of various human health parameters. The escalating demand for continuous and immediate surveillance of both acute and chronic conditions, compounded by constraints in clinical infrastructure, has propelled extensive investigation into wearable biosensors. These systems have emerged as pivotal tools for furnishing physiological insights and facilitating non-invasive monitoring. They monitor various analytes through the analysis of sweat, epidermal, Interstitial fluids, breath and tears, and easily incorporated into flexible substrates ensuring long-term wear and continuous monitoring. However, these biosensors penetrate deeper into the body's fluid networks, providing a systemic approach to continuous monitoring of biomarkers, which is particularly useful for individuals with metabolic disorders. This comprehensive review explores recent trends and diverse applications of wearable biosensors. These applications empower individuals to dynamically assess their well-being, enabling informed healthcare decisions tailored to individual needs. The convergence of these approaches in wearable biosensors highlights a comprehensive approach to understand individual health. This integration holds the promise of transforming healthcare practice by providing continuous observance and remote monitoring. The result has been a paradigm shift towards active, personalized health care, leading to improved health outcomes and improved quality of life</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100486"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical imprinting meets nanotechnology: Ultra-sensitive monitoring of beta-endorphin","authors":"Ankita Ghosh ,&nbsp;Ramesh Chandra ,&nbsp;Nidhi Chauhan","doi":"10.1016/j.talo.2025.100529","DOIUrl":"10.1016/j.talo.2025.100529","url":null,"abstract":"<div><div>Beta-endorphin (BE) is an endogenous opioid peptide (EOP) produced in the anterior pituitary gland and hypothalamus. Beta-endorphin is a vital neurotransmitter involved in physiological processes including relief the pain, boosting memory, and regulating mood. BE is a happy hormone that reduces the chance of depression and anxiety. BE analysed by traditional laboratory techniques like LC-MS, ELISA and radioimmunoassay is reliable; however, there are certain limitations including time consumption, device complexity, cost and availability. This study aims to develop a self-monitoring molecularly imprinted polymer-based (MIP) biosensor for sensitive, selective and rapid detection of BE. The nanocomposite TiO₂/MoS₂ was electrochemically deposited on the screen-printed electrode using cyclic voltammetry (CV) to enhance the conductivity and surface area to immobilize MIP. The MIP was electrochemically deposited at voltage range 0.2 to -0.6 V using CV for 20 cycles at scan rate 50 mV/s on the modified TiO₂/MoS₂/SPE. MIP is a synthetic bio-recognizing element that provides high selectivity for BE over other structurally similar molecules. The developed sensor MIP@TiO₂/MoS₂/SPE exhibits sensitivity of 0.475 µA/pM, the limit of detection (LOD) of 0.1pM and a detection range of 0.1pM and 200pM. This is novel, innovative and cost-effective point of care device developed for the rapid and real time detection of BE.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100529"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144887024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical detection of enrofloxacin and ciprofloxacin based on mesoporous La2O2CO3 nanostructures – an eco-friendly electrode material for sustainability","authors":"Arghya Chakravorty ,&nbsp;Sudip Das ,&nbsp;Sahil Luktuke ,&nbsp;Aarcha Appu Mini ,&nbsp;Vimala Raghavan","doi":"10.1016/j.talo.2025.100546","DOIUrl":"10.1016/j.talo.2025.100546","url":null,"abstract":"<div><div>Administration of antibiotics in the modern world has posed a serious threat to induce antimicrobial resistance (AMR), which concerns the Sustainable Development Goals (SDGs). Fluoroquinolone group antibiotics, namely enrofloxacin and ciprofloxacin, are predominantly responsible for undermining the One Health. In this context, to provide an eco-friendly and robust approach, a green electrochemical sensor has been fabricated with lanthanum oxycarbonate (La₂O₂CO₃) nanoparticles for the detection of enrofloxacin and ciprofloxacin in several complex matrices, related to food safety, environmental monitoring, and human health. The green synthesis methodology was adopted for the synthesis of the oxycarbonate material, while the phytochemicals present in the leaves of <em>Sida acuta</em> played the role of reducing and capping agents. To characterize the crystallinity and morphology of the synthesized material, several spectroscopic (XRD, BET, EDAX) and microscopic (FESEM, TEM) techniques were employed. The fabricated electrodes were characterized by atomic force microscopy (AFM), goniometry, and surface profilometry. The fabricated voltammetric sensor demonstrated an efficient linear range of 2.77 µM to 158.99 µM and 5.98 µM to 57.87 µM in the case of enrofloxacin and ciprofloxacin, respectively. In both cases, cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) have been employed as electrochemical analytical techniques. The limit of detection (LOD) was achieved to be 4.07 µM, 2.91 µM, and 4.98 µM, while the limit of quantification (LOQ) was achieved to be 14.2 µM, 8.83 µM, and 15.1 µM in CV, DPV, and SWV, respectively, for the detection of enrofloxacin. On the other hand, the LOD was achieved to be 2.85 µM, 1.90 µM, and 1.65 µM, while the LOQ was found as 8.64 µM, 5.7 µM, and 5.01 µM in CV, DPV, and SWV, respectively, for the detection of ciprofloxacin.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100546"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}