Talanta Open最新文献

{"title":"Conducting polymer-based electrochemical sensors: Progress, challenges, and future perspectives","authors":"Aashutosh Dube ,&nbsp;Shweta J. Malode ,&nbsp;Abdullah N. Alodhayb ,&nbsp;Kunal Mondal ,&nbsp;Nagaraj P. Shetti","doi":"10.1016/j.talo.2024.100395","DOIUrl":"10.1016/j.talo.2024.100395","url":null,"abstract":"<div><div>Conducting polymers are promising due to their unique properties, such as excellent electrical and optical properties, physical and chemical stability, high conductivity, and effective redox properties with high-temperature stability and biocompatibility. Due to these properties, conducting polymers are useful in diverse applications like sensors, batteries, oil industries, biosensors, biomedicines, catalysis, cancer treatment, etc. This review article aims to discuss the recent trends and analysis of conducting polymer-based electrochemical sensors in diverse areas with all required sensor characteristics, such as the derived limit of detection, utilized techniques for the sensing analysis and derived linear dynamic range with the stability of the sensors. Conducting polymers and their nanocomposites-based electrochemical sensors have demonstrated exceptional capabilities towards detecting various biomolecules, heavy metals, pesticides, and viruses like SARS-COV-2. Incorporation of redox mediators, use of conducting hydrogels, and molecular imprinting are promising strategies for better performance of the derived sensor. The article has demonstrated the existing challenges and limitations and provided solutions in the field. In the future, conducting polymers-based electrochemical sensors can be utilized in wearable sensors and integrated with IoT devices for better reach in real-time applications. They can also be made more accessible with precise control and data output by following specific methodologies. Utilizing green and sustainable conducting polymers can be crucial in advancing eco-friendly practices in the future. Conducting polymer-based electrochemical sensors has affectivity in neurochemical and pathogen sensing, which is essential for brain function and mental health.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100395"},"PeriodicalIF":4.1,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in metal-organic frameworks for electrochemical sensing applications","authors":"Magesh Kumar Muthukumaran ,&nbsp;Muthukumar Govindaraj ,&nbsp;Sakthivel Kogularasu ,&nbsp;Balasubramanian Sriram ,&nbsp;Bharathi Kannan Raja ,&nbsp;Sea-Fue Wang ,&nbsp;Guo-Ping Chang-Chien ,&nbsp;Arockia Selvi J","doi":"10.1016/j.talo.2024.100396","DOIUrl":"10.1016/j.talo.2024.100396","url":null,"abstract":"<div><div>Metal-organic frameworks (MOFs) have emerged as promising electrode modifiers in electrochemical sensing owing to their unique structural attributes, such as high surface area, tunable porosity, high catalytic activity, and abundant active sites. These properties make MOF-based systems highly effective for detecting a wide range of analytes, including heavy metals, antibiotics, environmental pollutants, and biomarkers. MOFs offer rapid, cost-effective analysis, yet challenges remain in optimizing their electrochemical properties to fully meet the demands of practical applications, particularly in energy conversion and storage (e.g., supercapacitors, batteries, and water-splitting catalysts) and in the fabrication of high-performance electrochemical sensors. This review critically examines the electrochemical properties of MOF-based materials for detecting various analytes, exploring their current limitations and potential for improvement. Particular focus is given to the design and synthesis strategies that enhance MOFs' structural and electrochemical properties, making them more suitable for real-world applications. Furthermore, this review highlights the challenges associated with MOF stability and conductivity and suggests pathways for overcoming these barriers. Reviewing recent advancements in MOF synthesis and functionality, this article provides a comprehensive overview of how MOFs can be developed as next-generation electrochemical sensing platforms. It also discusses future perspectives, including integrating MOFs into multifunctional sensors and their potential role in wearable and IoT-enabled devices. This review bridges the gap between theoretical research and practical applications, offering valuable insights into the future of MOF-based electrochemical technologies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100396"},"PeriodicalIF":4.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Europium-based core-shell materials for fluorescence and colorimetric dual-mode sensing of alkaline phosphatase activity","authors":"Chen-Xi Zhao,&nbsp;Xiao-Xia Li,&nbsp;Yang Shu","doi":"10.1016/j.talo.2024.100397","DOIUrl":"10.1016/j.talo.2024.100397","url":null,"abstract":"<div><div>Abnormal alkaline phosphatase levels have been associated with several diseases, including tumor growth, diabetes, liver dysfunction and so on. Generally, the occurrence and prognosis of these diseases can be reflected by the serum alkaline phosphatase level. In this work, Eu/DBM@SiO₂@SiO₂ is prepared to protect the internal Eu complex from the external environment by wrapping Eu/DBM inside the silica shell. Then, Eu/DBM@SiO₂@SiO₂/MnO₂, a sensor consists of Eu/DBM@SiO₂@SiO₂ and MnO₂, is prepared and applied for alkaline phosphatase detection. MnO₂ is used as the response part of alkaline phosphatase, which can be degraded to Mn²⁺ by ascorbic acid. Ascorbic acid is the hydrolysate of alkaline phosphatase. In the process of this reaction, UV–vis absorption of Eu/DBM@SiO₂@SiO₂/MnO₂ is reduced and fluorescence is turned on. Alkaline phosphatase concentration is linearly correlated with the logarithmic value of fluorescence enhancement in the range of 10.0–100.0 U/L with linear equation of Lg(F/F₀)=0.008C-0.030 (R²=0.99). For fluorescence method, the LOQ is 10.0 U/L and the LOD is 4.4 U/L. For colorimetric method, the concentration of alkaline phosphatase is linearly correlated with the decrease of absorption intensity in the range of 20.0–90.0 U/L with the linear equation of A/A₀=-0.012C+1.148 (R²=0.99). The LOQ is 20.0 U/L and the LOD is 7.2 U/L. The detection system has good selectivity and can detect alkaline phosphatase in human serum samples, the accuracy of the detection system is verified by the experiment of spike-in experiment. The RSD of alkaline phosphatase concentration in serum samples is in the range of 1.4–8.4 %. This method has potential application in the detection of ALP activity in biological samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100397"},"PeriodicalIF":4.1,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in field effect transistor based electronic devices integrated with CMOS technology for biosensing","authors":"Harshita Rai ,&nbsp;Kshitij RB Singh ,&nbsp;Arunadevi Natarajan ,&nbsp;Shyam S. Pandey","doi":"10.1016/j.talo.2024.100394","DOIUrl":"10.1016/j.talo.2024.100394","url":null,"abstract":"<div><div>This review article embarks on an enlightening journey through the multifaceted realm of electronic devices and their applications in biosensing, emphasizing the role of Field effect transistor (FET) based biosensors and Complementary Metal Oxide Semiconductor (CMOS) processes in biosensing device development. It begins by elucidating the foundational principles of biosensing and underscoring the crucial contribution of transducers, establishing a robust understanding of the field. The article unravels the intricate interplay between electronic biosensors and CMOS processes, offering a concise yet insightful exploration of their operational intricacies, diverse practical applications, and recent advancements. Additionally, it spotlights the pivotal role of FET-based biosensors integrated with CMOS processes in miniaturizing biosensors and thus amplifying their real-world efficacy. Moreover, the role of modern technologies, such as the Internet of Things (IoT), in recent biosensor development has been discussed. By addressing inherent challenges like sensitivity, integration, cost, and accessibility, the article underscores the vital role of biosensing technologies driven by electronic devices in wearable technology development. In addition, integrating these devices to fit with the ongoing trend of VLSI technology faces significant challenges. To overcome this aspect, sensors based on molecularly imprinted polymers (MIPs) can be the best alternative, as they will avoid utilizing bioreceptors, as it simplifies integration by reducing complexity, enhancing stability, and improving compatibility with CMOS processes. Hence, this review's distinct contribution lies in its comprehensive approach, shedding light on how biosensing technologies, underpinned by electronic devices such as FETs and CMOS processes, offer solutions for realizing modern-day devices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100394"},"PeriodicalIF":4.1,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing an innovative, eco-conscious optode for spectrophoto-metric detection of copper across various biological and environmental samples","authors":"Abdullah H. Alluhayb ,&nbsp;Alaa M. Younis ,&nbsp;Alaa S. Amin ,&nbsp;Naglaa Mohamed","doi":"10.1016/j.talo.2024.100393","DOIUrl":"10.1016/j.talo.2024.100393","url":null,"abstract":"<div><div>An environmentally sustainable optode has been developed for the detection of copper ions in water-based solutions. The optode was created by immobilizing 2-[6-nitro-2-benzothiazolylazo]-4-hydroxybenzoic acid (NBTHB) onto a porous cellulosic polymer membrane. This immobilization technique is especially beneficial for dye molecules that tend to degrade in alkaline ethylenediamine solutions, which are frequently employed during optode fabrication. The optode demonstrated a linear response to copper(II) ions within the concentration range of 10 to 130 ng mL^–1, achieving a high correlation coefficient of 0.9990. The quantification and detection limits were determined to be 10 and 3.0 ng mL^–1, respectively. The precision results were reported as % RSD, with intra-day values of 2.30% and 1.65%, and inter-day values of 2.15% and 1.85%, respectively. The selectivity of the sensor membrane was rigorously tested for a various cations and anions, successfully establishing the tolerance limits for interfering species. It could be effectively regenerated by treatment with 0.1 M EDTA, restoring its functionality for repeated use. The optode was effectively employed to measure copper(II) ions in a range of food products, biological fluids, and environmental water samples. The results obtained were comparable to those achieved using the ICP-AES technique, demonstrating the optodes reliability and accuracy.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100393"},"PeriodicalIF":4.1,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the potential application of single-walled carbon nanotubes in medical treatment and therapy","authors":"Sandra Ross Olakkengil Shajan ,&nbsp;Nandini Markuli Sadashivappa ,&nbsp;Devaraj Hanumanthappa ,&nbsp;Shivaraj Kumar Walikar ,&nbsp;Basavana Gowda Hosur Dinesh ,&nbsp;Bandral Sunil Kumar ,&nbsp;Selvaraj Kunjiappan ,&nbsp;Panneerselvam Theivendren ,&nbsp;Santhana Krishna Kumar Alagarsamy ,&nbsp;Kumarappan Chidamabaram ,&nbsp;Damodar Nayak Ammunje ,&nbsp;Parasuraman Pavadai","doi":"10.1016/j.talo.2024.100392","DOIUrl":"10.1016/j.talo.2024.100392","url":null,"abstract":"<div><div>This comprehensive review highlights the potential of carbon nanotubes (CNTs) as versatile nanomaterials in medicine by exploring their numerous therapeutic applications. The synthesis processes for single-walled and multi-walled carbon nanotubes are explored in detail in this article, which includes flame synthesis, arc discharge method, laser ablation, nebulized spray pyrolysis, and chemical vapor deposition method. The article focuses on the unique physiochemical characteristics of CNTs that make them attractive for a range of biological uses, including genetic engineering, infection therapy, antibiotics, antibacterial treatments, and anticancer therapies. This article also examines the potential use of CNTs in tissue regeneration and artificial implantation, as well as their use as medications and drug delivery vehicles. The importance of functionalized carbon nanotubes in improving biological applications- such as tissue engineering and infection treatment-is emphasized in the study. Furthermore, it also discusses the possible drawbacks and toxicity issues related to the application of CNTs, highlighting the need for more studies to guarantee their safety and efficient application in therapeutic contexts.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100392"},"PeriodicalIF":4.1,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrotropy and co-solvency: Sustainable strategies for enhancing solubility of poorly soluble pharmaceutical active ingredients","authors":"Mohamed A. El Hamd ,&nbsp;Reem H. Obaydo ,&nbsp;Dania Nashed ,&nbsp;Mahmoud El-Maghrabey ,&nbsp;Hayam M. Lotfy","doi":"10.1016/j.talo.2024.100391","DOIUrl":"10.1016/j.talo.2024.100391","url":null,"abstract":"<div><div>This review explores sustainable strategies for enhancing the solubility of poorly water-soluble pharmaceutical active ingredients (PAIs), focusing on hydrotropy and co-solvency, while comparing their effectiveness, sustainability, and applicability in analytical chemistry, particularly within the frameworks of green and white analytical chemistry (GAC and WAC). Methods like hydrotropy, co-solvency, solid dispersions, and pH modification are discussed, each with distinct advantages and limitations. Hydrotropy, introduced by Carl A. Neuberg in 1916, enhances solubility without altering a drug's UV measurement range and is known for its eco-friendliness, stability, and scalability. Co-solvency modifies the solvent environment using solvents like ethanol but poses environmental concerns. Hydrotropy is safer and more scalable, though it requires more additives, while co-solvency is cost-effective but presents toxicity and precipitation risks. The environmental and safety benefits of hydrotropic solvents align with global sustainability initiatives like the UN SDGs. A case study comparing two UV methods (one with organic solvents and another with hydrotropic solvents) showed hydrotropy's superiority in sensitivity, stability, and sustainability, achieving a sustainability score of 71 % versus 29 % for methanol. The review also highlights the synergistic effects of multiple hydrotropic agents in improving solubility and drug stability. Overall, hydrotropy, particularly when combined with other solubility-enhancing techniques, represents a promising, sustainable approach for improving PAI solubility, offering significant safety, environmental, and economic benefits over traditional organic solvents.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100391"},"PeriodicalIF":4.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Far-field ammonia gas sensing at room temperature with graphene nanoplatelets-infused PEDOT:PSS transparent thin film","authors":"Vinod K Ganesan ,&nbsp;Chun Hui Tan ,&nbsp;Pei Song Chee ,&nbsp;Jen Hahn Low ,&nbsp;Soon Poh Lee ,&nbsp;Eng Hock Lim","doi":"10.1016/j.talo.2024.100389","DOIUrl":"10.1016/j.talo.2024.100389","url":null,"abstract":"<div><div>Amid the increasing demand for advanced gas sensing technologies, particularly for ammonia gas detection, this study presents an innovative solution for far-field sensing at room temperature. Widely used in various industrial applications, ammonia poses significant environmental and health risks, emphasizing the need for efficient monitoring. Although traditional gas sensing methods effective, they often constrained by high operating temperatures and complex electronic components, limiting their practicality. In response, transparent thin films have emerged as a promising alternative, offering real-time monitoring capabilities. However, existing transparent films often rely on external stimuli for activation, resulting in higher power consumption and degradation over time. This research investigates a transparent thin film composed of poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) incorporated with graphene nanoplatelets (GNPs) for far-field ammonia gas sensing. Synthesized through a low-temperature, full-solution approach, the film demonstrates an average transmittance of 76.18 % in visible spectrum. Notably, patterning this film into a single slot antenna exhibits a significant 60 MHz frequency shift at a far-field distance of 12 cm when exposed to 50 ppm of ammonia gas. This significant frequency shift underscores the potential of the developed transparent slot antenna for practical and unobtrusive far-field sensing, advancing transparent gas sensors applications in environmental monitoring and workplace safety.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100389"},"PeriodicalIF":4.1,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-sensitive refractive index detection with gold-coated PCF-based SPR sensor","authors":"Amit Das ,&nbsp;Md Abu Huraiya ,&nbsp;Vinoth Raj R ,&nbsp;Hitoshi Tabata ,&nbsp;Sankar Ganesh Ramaraj","doi":"10.1016/j.talo.2024.100384","DOIUrl":"10.1016/j.talo.2024.100384","url":null,"abstract":"<div><div>This study introduces an innovative photonic crystal fiber (PCF) surface plasmon resonance (SPR) biosensor, notable for its sophisticated design and exceptional performance, utilizing gold as the plasmonic material. The sensor offers an expanded detection range and outstanding sensitivity, operating within a refractive index range of 1.27 to 1.43. It achieves a remarkable wavelength sensitivity of 157,000 nm/RIU with a resolution of 6.37 × 10⁻⁷ RIU and an amplitude sensitivity of 1263 RIU⁻¹ with a resolution of 7.92 × 10⁻⁵ RIU. Additionally, it boasts a figure of merit (FOM) of 1648, underscoring its superior capability in detecting biomolecular interactions. The suggested design is numerically analysed using the finite element method (FEM) of a gold-coated PCF-based SPR sensor designed for detecting changes in the refractive index (RI) within the range of 1.27 to 1.43. This range is related to various biological and chemical samples, including proteins, DNA, and biochemical analytes typically used in biomedical diagnostics and environmental monitoring. The integration of gold plasmonic elements enhances the sensor's sensitivity and stability while extending its detection capabilities across a broader range, allowing for versatile applications in biomedicine, environmental monitoring, and beyond. This research significantly advances optical biosensing technologies by offering a robust platform for high-precision detection across diverse domains. The sensor's advanced design and performance make it an invaluable tool for applications requiring accurate and reliable detection, driving innovations in areas such as biomedical diagnostics, environmental monitoring, food safety, and pharmaceutical quality control.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100384"},"PeriodicalIF":4.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel sustainable, portable, fast, and non-invasive platform based on ATR-FTIR technology coupled with machine learning algorithms for Helicobacter pylori detection in human saliva","authors":"Ghabriel Honório-Silva ,&nbsp;Marco Guevara-Vega ,&nbsp;Nagela Bernadelli Sousa Silva ,&nbsp;Marcelo Augusto Garcia-Júnior ,&nbsp;Deborah Cristina Teixeira Alves ,&nbsp;Luiz Ricardo Goulart ,&nbsp;Mario Machado Martins ,&nbsp;André Luiz Oliveira ,&nbsp;Rui Miguel Pinheiro Vitorino ,&nbsp;Thulio Marquez Cunha ,&nbsp;Carlos Henrique Gomes Martins ,&nbsp;Murillo Guimarães Carneiro ,&nbsp;Robinson Sabino-Silva","doi":"10.1016/j.talo.2024.100383","DOIUrl":"10.1016/j.talo.2024.100383","url":null,"abstract":"<div><div><em>Helicobacter pylori (H. pylori</em>) infection can increase the risk of peptic ulcers and gastric neoplasms. <em>H. pylori</em> detection in gastric epithelial tissue collected by esophagogastroduodenoscopy (EGD) is an invasive, costly, and stands as an invasive and examiner-dependent procedure necessitating suitable sedation. complex execution procedure, reducing access for isolated populations. <em>H. pylori</em> detection by Urea Breath Test (UBT) presents high outlay cost with limited access in low- and middle-income countries. In this context, it is critical to develop novel alternative non-invasive platforms for the portable, fast, accessible through self-collection and reagent-free detection of <em>H. pylori</em>. Here, we used attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) supported by Machine Learning algorithms to identify infrared vibrational modes of <em>H. pylori</em> diluted in human saliva. To perform it, saliva was diluted in 4 different concentrations (10⁸ CFU/mL, 10⁷ CFU/mL, 10⁶ CFU/mL, and 10⁵ CFU/mL) of <em>H. pylori.</em> Then, diluted saliva with or without <em>H. pylori</em> were applied to ATR-FTIR spectroscopy to perform a reagent-free, fast, and sustainable analysis of spectral signatures to identify unique vibrational modes to identify this pathogen. The obtained spectra were applied to Linear Discriminant Analysis (LDA) and Support Vector Machine (SVM) algorithms to perform the <em>H. pylori</em> detection. The results indicate that the method was highly accurate between 10⁸ - 10⁵ CFU/mL, achieving an accuracy of 89 % for 10⁸ CFU/mL, 93 % for 10⁷ CFU/mL, 94 % for 10⁶ CFU/mL, and 85 % for 10⁵ CFU/mL with SVM algorithm. This proof-of-concept study demonstrates the significant potential of a biophotonic platform supported by artificial intelligence for the non-invasive detection of <em>H. pylori</em> in human saliva samples obtained by self-collection, without the use of reagents. The data reveal that this proof-of-concept study has significant potential for the non-invasive detection of <em>H. pylori</em> using a biophotonic platform supported by artificial intelligence without the use of reagents with human saliva samples obtained by self-collection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100383"},"PeriodicalIF":4.1,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}