Talanta Open最新文献_第10页

Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament 利用3D打印机和市售蜡丝制造纸基微流控装置

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100142

Antonio Espinosa, Joannes Diaz, Edgar Vazquez, Lina Acosta, Arianna Santiago, Lisandro Cunci

{"title":"Fabrication of paper-based microfluidic devices using a 3D printer and a commercially-available wax filament","authors":"Antonio Espinosa, Joannes Diaz, Edgar Vazquez, Lina Acosta, Arianna Santiago, Lisandro Cunci","doi":"10.1016/j.talo.2022.100142","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100142","url":null,"abstract":"<div>In this work, we developed an alternative manufacturing paper-based microfluidics method through 3D printing and wax filament. Microfluidic paper-based analytical devices (µPADs) are low-cost and easy-to-manufacture tools used for various chemical and biological analyses and studies. Paper-based microfluidics with wax has been limited as the manufacturers have discontinued most wax printing equipment. We aim to develop a low-cost and accessible manufacturing method that can replace conventional wax-on paper-based microfluidic manufacturing methods. Using highly available commercial 3D printing technology and wax filament, we could create hydrophobic wax barriers on the surface of different paper types. The properties and limits of this manufacturing method were characterized. Moreover, using this paper-based microfluidic manufacturing method, we were able to measure dopamine electrochemically using µPAD as a passive flow-based method in concentrations as low as 1 nM using injections as small as 15 µL.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100142"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1825617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development of a microfluidic paper-based analytical device for magnesium determination in saliva samples 唾液样品中镁的微流控纸基分析装置的研制

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100135

Juliana I.S. Aguiar, Mafalda T.S. Silva, Helena A.G. Ferreira, Elisabete C.B. Pinto, Marta W. Vasconcelos, António O.S.S. Rangel, Raquel B.R. Mesquita

{"title":"Development of a microfluidic paper-based analytical device for magnesium determination in saliva samples","authors":"Juliana I.S. Aguiar, Mafalda T.S. Silva, Helena A.G. Ferreira, Elisabete C.B. Pinto, Marta W. Vasconcelos, António O.S.S. Rangel, Raquel B.R. Mesquita","doi":"10.1016/j.talo.2022.100135","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100135","url":null,"abstract":"<div>In this work, a microfluidic paper-based analytical device (μPAD) was developed for magnesium determination in saliva samples. The idea was to develop a fast and simple method for biological magnesium quantification targeting saliva as an easy to collect and non-invasive alternative to blood or urine samples. The μPAD approach was chosen due to its advantages, namely ideally suited to conduct on-location determinations, and not requiring trained operators or specialized laboratory equipment. The developed μPAD was based on the colorimetric reaction between eriochrome cyanine and magnesium to form an intense orange/reddish colour product. The colour intensity was determined by image processing after digital scanning, made within 10 to 90 min after sample loading. Under optimal conditions, the dynamic concentration range was 82–247 μM, with detection and quantification limits of 62 μM and 81 μM, respectively. The device is stable for up to 3 months when stored in vacuum or in a modified nitrogen atmosphere. An accuracy assessment was made by comparing the results obtained using the developed μPAD with those from atomic absorption spectrometry (AAS). The relative difference between the two sets of results was below 5%.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3201169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A homogeneous biosensor for carbohydrate antigen 153 detection in human serum based on upconversion fluorescence resonance energy transfer 基于上转换荧光共振能量转移的人血清碳水化合物抗原153检测同质生物传感器

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100154

Tao Liang , Xiuqing Ma , Xiaojing Zhang, Miantai Ye, Yanying Wang, Chunya Li

{"title":"A homogeneous biosensor for carbohydrate antigen 153 detection in human serum based on upconversion fluorescence resonance energy transfer","authors":"Tao Liang , Xiuqing Ma , Xiaojing Zhang, Miantai Ye, Yanying Wang, Chunya Li","doi":"10.1016/j.talo.2022.100154","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100154","url":null,"abstract":"<div>Quantitative determination of carbohydrate antigen 153 (CA153) in human serum plays an important role in early diagnosis of breast cancer. In this work, a highly sensitive and selective biosensor for CA153 detection was developed based on a fluorescent resonance energy transfer (FRET) strategy, in which near-infrared (NIR)-excitable upconversion nanoparticles (UCNPs) and the commercial organic dye, FAM, were employed as energy donor and energy acceptor, respectively. The two components were linked by a molecular beacon (MB) containing CA153 aptamer sequence and the upconversion fluorescence (UCF) of UCNPs can be effectively quenched by FAM. In presence of CA153, the hairpin structure was opened, resulting in the separation of UCNPs and FAM and the inhibition of FRET. The UCF showed a linear relationship with the logarithm of CA153 concentration in the range from 0.01 to 150 U/mL, with a LOD of 4.5 mU/mL in HEPES buffer. This probe can be successfully applied for CA153 assay in human serum, which may be a useful tool for early diagnosis of breast cancer.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100154"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1825627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive method for determination of nimesulide and its hydroxy metabolite in milk using validated UPLC-MS method 高效液相色谱-质谱法测定牛奶中尼美舒利及其羟基代谢物

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100160

Bhoopendra Singh Kushwah , Laximan Ganesh Velip , Kala Kumar Bharani , Prasad V. Surya , Suprita Sinha , Amit Khurana , Yogeshwar Kankarne , Anil Kumar Banothu , Samanthula Gananadhamu

{"title":"A sensitive method for determination of nimesulide and its hydroxy metabolite in milk using validated UPLC-MS method","authors":"Bhoopendra Singh Kushwah , Laximan Ganesh Velip , Kala Kumar Bharani , Prasad V. Surya , Suprita Sinha , Amit Khurana , Yogeshwar Kankarne , Anil Kumar Banothu , Samanthula Gananadhamu","doi":"10.1016/j.talo.2022.100160","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100160","url":null,"abstract":"<div>Nimesulide is a widely used drug for the treatment of pain in humans as well as in animals. The presence of trace amounts of drugs in milk or other food supplement affects seriously human health. This study reports a method based on LC-MS detection for the determination of nimesulide and its hydroxy metabolite in cow milk samples. Waters ACQUITY BEH C18 column (100 mm × 2.1 mm, 1.7 µm), with gradient elution using 0.1% formic acid and acetonitrile as mobile phase at a flow rate of 0.3 mL min−1 was used for the chromatographic separation. The ion scans were quantified at m/z 307.0393 for nimesulide, m/z 323.0375 for hydroxy nimesulide metabolite and m/z 434.2198 for IS (valsartan) in negative ESI mode. A double extraction method with protein precipitation by acetonitrile followed by solid-phase extraction was developed to detect nimesulide at 1.30 ng mL−1 and hydroxy nimesulide at 1.03 ng mL−1 in milk. The calibration curve was constructed for nimesulide in the range of 1.30 ng mL−1 to 125.07 ng mL−1 while for hydroxy nimesulide calibration curve was constructed in the range of 1.03 ng mL−1 to 99.70 ng mL−1 in whole cow milk without any treatment. The validation parameters like accuracy, precision, selectivity, stability, matrix effect and dilution integrity were also performed on nimesulide and hydroxy nimesulide. The developed processing and chromatographic method can be used to quantitatively determine nimesulide or its hydroxy nimesulide metabolite in biological matrices, i.e., milk, plasma, faecal, tissue, etc.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100160"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2702809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ultrasensitive assay of HER1, HER2, and heregulin-α in whole blood 全血中HER1、HER2和herigulin -α的超灵敏测定

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100151

Raluca-Ioana Stefan-van Staden , Oana-Raluca Musat , Damaris-Cristina Gheorghe , Ruxandra-Maria Ilie-Mihai , Catalina Cioates Negut , Paula Sfirloaga

{"title":"Ultrasensitive assay of HER1, HER2, and heregulin-α in whole blood","authors":"Raluca-Ioana Stefan-van Staden , Oana-Raluca Musat , Damaris-Cristina Gheorghe , Ruxandra-Maria Ilie-Mihai , Catalina Cioates Negut , Paula Sfirloaga","doi":"10.1016/j.talo.2022.100151","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100151","url":null,"abstract":"<div>Microsensors based on the physical immobilization of oleamides (N-(pyridine-3-yl-methyl) oleamide, and N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide) on paste containing nanographene decorated with spheroidal Cu were proposed for the molecular recognition and ultrasensitive simultaneous determination of HER1, HER2, and heregulin-α in whole blood. While for the assay of HER1 and HER2 there were not significant differences in the sensitivities recorded when N-(pyridine-3-yl-methyl) oleamide, and N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide were used to modify the nanographene paste, for the assay of heregulin-α, a higher sensitivity was recorded when N-(2-mercapto-1H-benzo[d]imidazole-5-yl) oleamide was used to modify the paste. Wide working concentration ranges were recorded for HER1 (between 10−13 and 10−8 mol L − 1), HER2 (between 10−12–10−10 mol L − 1), and heregulin-α (10−13–10−11mol L − 1). The student t-test showed that there is no significant difference between the results obtained by utilizing the two microsensors for screening tests of whole blood, at 99% confidence level.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100151"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1749539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Novel bis naphthalene-2-ol based colorimetric chemosensor for the detection of Fe2+ in physiological pH and its DFT calculation studies 新型二萘-2-醇基比色化学传感器用于生理pH中Fe2+的检测及其DFT计算研究

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100143

Rajendran Nagarajan , Eswaran Kamaraj , Chong-Hyeak Kim , Ki Hwan Lee

{"title":"Novel bis naphthalene-2-ol based colorimetric chemosensor for the detection of Fe2+ in physiological pH and its DFT calculation studies","authors":"Rajendran Nagarajan , Eswaran Kamaraj , Chong-Hyeak Kim , Ki Hwan Lee","doi":"10.1016/j.talo.2022.100143","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100143","url":null,"abstract":"<div>A novel bis naphthalene-2-ol-based multi-functional chromogenic chemosensor was synthesized in a single step and thoroughly characterized by Fourier-Transform Infrared Spectroscopy, Mass spectrometry, Nuclear Magnetic Resonance and Single Crystal-X-ray for the detection of Fe2+ and CN− ions in physiological pH. The detection limit of Fe2+ was initiated as 4.35 μM is much superior to the United States Environmental Protection Agency parameter value for drinking water (5.37 μM). The 3-Fe2+ complex we can reversibly reuse after the treatment with CN− ion. A noteworthy red-shifted with hypochromic shift spectrum and “naked eye” colour change has been observed from yellow colour to light pink colour within 5 s for Fe2+ ion but not for other competing metal ions. Without any complex pretreatment, our chemosensor easily discriminates Fe2+ and Fe3+ ions. From Job's plot analysis, 1H NMR titration and MS analysis confirm the binding stoichiometry among probe 3 and Fe2+ metal ion as 1:1. The experimental active molecular binding site results are correlated well with the Density Functional Theory calculation to understand the sensing mechanism.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100143"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3267881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The cyclic nature of soil chemistry: Forensic analysis with the aid of ultra-high performance liquid chromatography 土壤化学的循环性质:借助超高高效液相色谱法的法医分析

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100126

Jessica G.P. Hay , Andrew P.A. Oxley , Melissa L. Wos-Oxley , Robert Hayes , Tony Pickles , Ken Roberts , Xavier A. Conlan

{"title":"The cyclic nature of soil chemistry: Forensic analysis with the aid of ultra-high performance liquid chromatography","authors":"Jessica G.P. Hay , Andrew P.A. Oxley , Melissa L. Wos-Oxley , Robert Hayes , Tony Pickles , Ken Roberts , Xavier A. Conlan","doi":"10.1016/j.talo.2022.100126","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100126","url":null,"abstract":"<div>Despite its forensic importance as trace evidence, soil is under-utilised by forensic scientists due to a lack of research into the suitability of analytical approaches and a subsequent lack of understanding of the soil composition. Ultra-high performance liquid chromatography was used to analyse acetonitrile extractions from 210 soil samples taken from four locations across the Geelong and Surf Coast Shire areas of Victoria, Australia from March 2020 to January 2021. With the aid of principal components analysis of the UV-visible data chromatograms, it was found that this method could discriminate soil between locations, timepoints, and age points, and therefore, for the first time, shows that the method may offer a reliable method for forensic soil analysis. The approach used PCoA plots representing Bray-Curtis similarities comparing the UHPLC peak profiles which was able to show that the soil chemistry changes are cyclic, a factor that will help improve the utility of this approach for forensic analysis. As the separation was perfomred using a C18 column, the LDA scores suggest that hydrophobicity based on methyl selectivity may be important in spatial discrimination using this method. Further this type of approach generates a simple visual representation of the data that will be of value for presentation within a courtroom.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100126"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3450954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Green spectrofluorimetric determination of alendronate sodium using nano switchable probe; Water-soluble carbon dots synthesized from a natural source 纳米可切换探针绿色荧光法测定阿仑膦酸钠从天然来源合成的水溶性碳点

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100127

Mohamed Rizk, Maha Abou El-Alamin, Dina Mohamed, Safaa Toubar

引用次数: 0

New Raman spectroscopic methods’ application in forensic science 新型拉曼光谱方法在法医学中的应用

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100124

Elmer-Rico Mojica, Zhaohua Dai

引用次数: 3

EDTA-capped silver nanoparticles as a probe for highly sensitive and selective colorimetric sensing of creatinine and optimization using response surface methodology-Box Behnken Design edta覆盖银纳米颗粒作为高灵敏度和选择性肌酐比色检测探针，并使用响应面法优化- box Behnken设计

Talanta Open Pub Date : 2022-12-01 DOI: 10.1016/j.talo.2022.100170

Indah Miftakhul Janah , Roto Roto , Katsuaki Konishi , Dwi Siswanta

{"title":"EDTA-capped silver nanoparticles as a probe for highly sensitive and selective colorimetric sensing of creatinine and optimization using response surface methodology-Box Behnken Design","authors":"Indah Miftakhul Janah , Roto Roto , Katsuaki Konishi , Dwi Siswanta","doi":"10.1016/j.talo.2022.100170","DOIUrl":"https://doi.org/10.1016/j.talo.2022.100170","url":null,"abstract":"<div>Creatinine concentration is an essential indicator for assessing kidney function and diagnosing a disease. A selective and sensitive colorimetric detection was developed based on the EDTA-capped AgNPs aggregation to determine creatinine accurately. The developed method was optimized using response surface methodology (RSM) with Box-Behnken Design (BBD) model by studying pH, AgNPs concentration, and detection time as the critical parameters. The result showed that medium pH had the most crucial role in the interaction between EDTA-capped AgNPs and creatinine. The addition of creatinine to the colloidal EDTA-capped AgNPs induced the particle aggregation, followed by a color change from yellow (with lambda max 398 nm) to dark blue (with lambda max 650 nm) within 1 min of reaction at pH 12. The tautomerization of creatinine to its anionic amino species at alkaline pH, which cross-links the EDTA-capped AgNPs through the hydrogen bond networks with the negatively charged EDTA-capped AgNPs, occurs via an aggregation mechanism. The SPR absorbance ratio of A650/A398 was correlated to the creatinine concentration under the optimized conditions in the range of 0.0–3.0 μM with the limit of detection (LOD) of 0.0073 µM. The proposed method could offer simple, rapid, and inexpensive creatinine analysis for clinical diagnosis.</div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"6 ","pages":"Article 100170"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1631719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0