Talanta Open最新文献_第10页

Pigment-based biosensors for environmental monitoring 用于环境监测的色素生物传感器

IF 4.1

Talanta Open Pub Date : 2025-05-05 DOI: 10.1016/j.talo.2025.100471

Shivangini Desai, Sonal Desai

{"title":"Pigment-based biosensors for environmental monitoring","authors":"Shivangini Desai, Sonal Desai","doi":"10.1016/j.talo.2025.100471","DOIUrl":"10.1016/j.talo.2025.100471","url":null,"abstract":"<div><div>Environmental pollution is progressively escalating owing to an increase in population and industrialization. Various industrial wastes are discharged into the water, soil, and air. Consequently, there is an urgent need to monitor the levels of these pollutants in the environment, as they pose a threat not only to human health, but also to the ecosystem, affecting all organisms. A biosensor detects biological or chemical processes by generating signals proportional to the analyte concentration in the reaction. Biosensors are utilized to monitor Biochemical Oxygen Demand (BOD), pesticides, phenols, heavy metals, and polluting gases. Pigment-based biosensors facilitate colorimetric and visual detection without the need for complex instrumentation. These biosensors can produce a response either because the analyte reacts directly with the pigment or through the analyte interacting with a genetic pathway that leads to pigment production, resulting in colour change. Through the genetic engineering of microorganisms to produce specific pigments, specific heavy metal detection can be achieved. This study examines the applications of pigment-based biosensors in the environmental monitoring of various pollutants, including heavy metals and pesticides, in contrast to conventional detection techniques. It also explores the recent advancements and limitations.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100471"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of oleanolic acid and apigenin in biological matrices following intraperitoneal administration of co-loaded nanoformulations: Method validation and biodistribution studies 腹腔注射共载纳米制剂后生物基质中齐墩果酸和芹菜素的定量：方法验证和生物分布研究

IF 4.1

Talanta Open Pub Date : 2025-05-05 DOI: 10.1016/j.talo.2025.100470

Amna Naz , Sadia Haider , Mavra Rana , Neelum Malik , Attia Afzal , Muhammad Farooq , Muhammad Sarfraz

{"title":"Quantification of oleanolic acid and apigenin in biological matrices following intraperitoneal administration of co-loaded nanoformulations: Method validation and biodistribution studies","authors":"Amna Naz , Sadia Haider , Mavra Rana , Neelum Malik , Attia Afzal , Muhammad Farooq , Muhammad Sarfraz","doi":"10.1016/j.talo.2025.100470","DOIUrl":"10.1016/j.talo.2025.100470","url":null,"abstract":"<div><div>Oleanolic acid (OA) and apigenin (APG) demonstrate a wide range of pharmacological effects, including antioxidant, anti-inflammatory, antiviral, antidiabetic, hepatoprotective, and cardioprotective activities. Both compounds show anticancer potential and have been used individually to treat various cancer types, creating a rationale for their combined use. However, quantifying both drugs in quality and plasma samples presents challenges due to differences in their physicochemical properties and absorption wavelengths (OA: 210–215 nm, APG: 212, 269, and 337 nm). We developed and validated a novel High-Performance Liquid Chromatography UV detection (HPLC-UV) method to address this problem. Using a C18 column (4.6 mm x 250 mm, packing L1, 5 µm particle size) and a mobile phase of acetonitrile: methanol: tetrahydrofuran: water (60:20:8:12, v/v) with a detection wavelength of 215 nm. The method achieved retention times of 4.7 and 10 minutes for OA and APG in quality samples, and 4.9 and 9.8 minutes in plasma samples, respectively. The plasma sample was prepared by simple protein precipitation step. All validation parameters, including sensitivity, selectivity, precision, accuracy, robustness, and stability, met the required criteria. The validated HPLC-UV bioanalytical method was effectively applied in quantification, stability, and pharmacokinetic studies of co-loaded nanoformulations of OA and APG with particle size 163 nm. The entrapment efficiency for OA was 93.95 ± 3.50 %, and for APG, it was 94.5 ± 2.31 % in combined nanoformulation, establishing it as a promising analytical technique for detecting these anticancer agents in complex matrices such as blood, plasma, and polymeric systems.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100470"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A facile and rapid detection of Cr(VI) and ascorbic acid using N,S-co-functionalized carbon dots: A dual-mode 'on-off-on' fluorescent sensor 利用N， s共功能化碳点简便快速地检测Cr（VI）和抗坏血酸：一种双模式“开-关-开”荧光传感器

IF 4.1

Talanta Open Pub Date : 2025-05-05 DOI: 10.1016/j.talo.2025.100472

D. James Nelson , N.S.K. Gowthaman , Bharathi Sinduja , M.G. Sethuraman

{"title":"A facile and rapid detection of Cr(VI) and ascorbic acid using N,S-co-functionalized carbon dots: A dual-mode 'on-off-on' fluorescent sensor","authors":"D. James Nelson , N.S.K. Gowthaman , Bharathi Sinduja , M.G. Sethuraman","doi":"10.1016/j.talo.2025.100472","DOIUrl":"10.1016/j.talo.2025.100472","url":null,"abstract":"<div><div>This study reports the successful synthesis of nitrogen- and sulfur-co-functionalized carbon dots (N,S-CDs) using citric acid and L-cysteine through a microwave-assisted hydrothermal process. These N,S-CDs were employed for the quantification of dichromate ions (Cr(VI)) and ascorbic acid (AA) through a fluorescence “on-off-on” detection method. The N,S-CDs displayed deep blue fluorescence, with an emission peak at 420 nm upon excitation at 349 nm. The presence of Cr(VI) significantly quenched the fluorescence intensity, enabling its selective detection over a linear range of 1–40 μM with a limit of detection of 58 nM. The addition of AA restored the fluorescence intensity by reducing Cr(VI) to Cr(III), allowing for AA detection within a dynamic range of 5–90 μM with a detection limit of 180 nM. The fluorescence quenching by Cr(VI) involved both the inner filter effect (IFE) and static interactions and the IFE was reversed upon the introduction of AA, which restored the fluorescence. This method demonstrated excellent sensitivity and selectivity for detecting Cr(VI) and AA, with successful application in the analysis of real samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100472"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel ISE Modified with Synthesized Magnetite Nanoparticles for Sulfates Determination 合成纳米磁铁矿修饰的新型ISE测定硫酸盐

IF 4.1

Talanta Open Pub Date : 2025-05-04 DOI: 10.1016/j.talo.2025.100468

Ita Hajdin , Karmela Zanki Kulazo , Ante Prkić , Kristian Nakić , Andrea Paut , Lucija Guć , Mitja Kolar , Boštjan Genorio , Ivana Mitar

{"title":"Novel ISE Modified with Synthesized Magnetite Nanoparticles for Sulfates Determination","authors":"Ita Hajdin , Karmela Zanki Kulazo , Ante Prkić , Kristian Nakić , Andrea Paut , Lucija Guć , Mitja Kolar , Boštjan Genorio , Ivana Mitar","doi":"10.1016/j.talo.2025.100468","DOIUrl":"10.1016/j.talo.2025.100468","url":null,"abstract":"<div><div>Potentiometric ISEs are one of the fastest developing sensors in electrochemistry due to their wide range of applications. They are used in the food industry, agriculture, environmental and drug analysis as they offer numerous advantages: high selectivity and sensitivity, low LOD, small size, easy handling, portability and low cost. We have therefore developed a solid-state ISE for the potentiometric determination of sulfate ions. The membranes are based on a mixture of barium sulfate, silver sulfide, polymer and metal oxide nanoparticles. The mass ratio of BaSO<sub>4</sub>, Ag<sub>2</sub>S and PTFE in the membranes was 1 : 1 : 2, with the addition of various metal oxide NPs, such as Fe<sub>3</sub>O<sub>4</sub>, α-Fe<sub>2</sub>O<sub>3</sub>, Al<sub>2</sub>O<sub>3</sub> or ZnO, to the membrane composition. The membrane containing magnetite NPs showed a positive effect on the linear response range for sulfate anions. In particular, the membrane containing 0.6% magnetite NPs showed a linear potential change with a slope of 28.5 mV dec<sup>−1</sup>, which is very close to the theoretical Nernstian value (29.6 mV dec<sup>−1</sup>), with a regression coefficient of 0.9978 in the concentration range between 4.88 ∙ 10<sup>−5</sup> mol L<sup>−1</sup> and 1.00 ⸱ 10<sup>−2</sup> mol L<sup>−1</sup> with LOD 4.40 ∙ 10<sup>−5</sup> mol L<sup>−1</sup>. Finally, none of the membranes showed response to barium ions even at high concentrations (≥ 1.00 ∙ 10<sup>−3</sup> mol L<sup>−1</sup>). These results confirm that our newly developed potentiometric sensor has a high membrane selectivity for the determination of sulfate ions and, most importantly, that it is a fast, reliable and cost-effective tool.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100468"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nature-driven blue-emissive N, S-CDs: Harnessing sequential “switch-off-on” fluorescence signals for detection of chrysin and Al³⁺ along with cellular imaging versatility 自然驱动的蓝发射N， S-CDs：利用连续的“开关-打开”荧光信号来检测菊花素和Al³+以及细胞成像的多功能性

IF 4.1

Talanta Open Pub Date : 2025-05-03 DOI: 10.1016/j.talo.2025.100466

Maha Mohammad Abdel-Monem , Mohamed I. Walash , Asmaa Kamal El-Deen

{"title":"Nature-driven blue-emissive N, S-CDs: Harnessing sequential “switch-off-on” fluorescence signals for detection of chrysin and Al³⁺ along with cellular imaging versatility","authors":"Maha Mohammad Abdel-Monem , Mohamed I. Walash , Asmaa Kamal El-Deen","doi":"10.1016/j.talo.2025.100466","DOIUrl":"10.1016/j.talo.2025.100466","url":null,"abstract":"<div><div>Novel biocompatible blue emissive nitrogen and sulfur co-doped carbon dots (N, S-CDs) were synthesized <em>via in situ</em> microwave-assisted synthesis from snake melon (<em>Cucumis melo</em> var. <em>flexuosus</em>) for the first time. The N, S-CDs have been utilized for fluorimetric determination of chrysin (CHR). The fluorescence emitted by the N, S-CDs (λ<sub>em</sub> = 460 nm) decreased upon the addition of CHR due to dynamic quenching and the inner filter effect (IFE). The reduction in emission intensity exhibits a linear relationship over a concentration range of 2.0–275.0 μM with a low limit of detection (LOD) of 0.0837 μM. By contrast, the quenched fluorescence of the N, S-CD/CHR system was restored upon the addition of Al³⁺, which efficiently displaced CHR from the surface of the N, S-CDs, prompting fluorescence \"switch-on\". The retrieved fluorescence intensity was proportional to Al<sup>3+</sup> concentration over a range of 50.0–4000.0 μM, with a LOD of 2.96 μM. This sequential approach was efficiently utilized to determine CHR and Al<sup>3+</sup> in real pharmaceutical, biological, and environmental samples with accurate and precise findings. Furthermore, due to their minimal cytotoxicity, the N, S-CDs were also successful for cellular imaging on HepG2 cells. The proposed sensing platform provides several merits, including simplicity, cost-efficiency, long-lasting stability, high selectivity, and exceptional sensitivity. These findings not only contribute to the growing field of carbon-based nanomaterials but also offer a sustainable and multifunctional platform for environmental and biomedical applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100466"},"PeriodicalIF":4.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Zein-templated gold nanoclusters and their application as a fluorescent probe for tetracycline detection 玉米蛋白模板金纳米团簇的制备及其在四环素检测中的应用

IF 4.1

Talanta Open Pub Date : 2025-05-03 DOI: 10.1016/j.talo.2025.100467

Yun Bai , Gerile Aodeng , Jun Ai , Guirong Borijihan

引用次数: 0

Green analytical chemistry in gabapentin analysis: Methods and sustainability review 加巴喷丁分析中的绿色分析化学：方法和可持续性综述

IF 4.1

Talanta Open Pub Date : 2025-05-01 DOI: 10.1016/j.talo.2025.100465

Hemraj Sharma , Kanaka Parvathi Kannaiah , Reem Hasan Obaydo , Rita Keshishian , Hemanth Kumar Chanduluru , Kritika Bhattarai , Ashish Acharya

{"title":"Green analytical chemistry in gabapentin analysis: Methods and sustainability review","authors":"Hemraj Sharma , Kanaka Parvathi Kannaiah , Reem Hasan Obaydo , Rita Keshishian , Hemanth Kumar Chanduluru , Kritika Bhattarai , Ashish Acharya","doi":"10.1016/j.talo.2025.100465","DOIUrl":"10.1016/j.talo.2025.100465","url":null,"abstract":"<div><div>Gabapentin (GP), widely prescribed for seizures and neuropathic pain, requires effective therapeutic monitoring and pharmaceutical quality control. Current analytical methods, such as UV spectrophotometry, HPLC, GC–MS, and LC-MS/MS, often rely on environmentally hazardous solvents and high-energy processes. This review evaluates analytical methods for GP till 2024, focusing on their eco-friendliness using green analytical chemistry (GAC) metrics. The study highlights the lack of concern for solvent toxicity, energy consumption, and waste generation in existing methodologies. Greenness assessment tools like GAPI, MoGAPI, AGREE, BAGI, AES reveal significant gaps in sustainability. This review underscores the need to develop greener analytical techniques for GP and advocate for eco-friendly solvents, reduced waste, and optimized energy use. Integrating GAC principles into analytical practices can enhance sustainability and robustness, reducing the environmental footprint of GP analysis. By compiling this information, the review aims to guide researchers in adopting innovative, eco-conscious methodologies, and aligning analytical procedures with sustainable chemistry goals.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100465"},"PeriodicalIF":4.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selectivity towards methyl mercaptan of Pt-PtO functionalized WO3 nanostructures made by flame spray pyrolysis 火焰喷雾热解制备Pt-PtO功能化WO3纳米结构对甲基硫醇的选择性

IF 4.1

Talanta Open Pub Date : 2025-04-29 DOI: 10.1016/j.talo.2025.100464

Kanittha Inyawilert , Matawee Punginsang , Chonlada Dechakiatkrai Theerakarunwong , Anurat Wisitsoraat , Chaikarn Liewhiran

{"title":"Selectivity towards methyl mercaptan of Pt-PtO functionalized WO3 nanostructures made by flame spray pyrolysis","authors":"Kanittha Inyawilert , Matawee Punginsang , Chonlada Dechakiatkrai Theerakarunwong , Anurat Wisitsoraat , Chaikarn Liewhiran","doi":"10.1016/j.talo.2025.100464","DOIUrl":"10.1016/j.talo.2025.100464","url":null,"abstract":"<div><div>This work presented 0–1 wt% Pt-PtO functionalized WO<sub>3</sub> nanostructures synthesized by flame spray pyrolysis (FSP) for methyl mercaptan (CH<sub>3</sub>SH)-sensing applications. According to electron microscopic and X-ray spectroscopic analysis, 1–3 nm Pt-PtO core-shell nanoparticles were decorated on 5–20 nm primary monoclinic WO<sub>3</sub> nanoparticles. The gas-sensing data revealed that Pt-PtO loading with an optimum Pt content of 0.5 wt% provided a remarkably enhanced CH<sub>3</sub>SH response of 204 to 500 ppb CH<sub>3</sub>SH at the best sensing temperature of 250°C with a low detection limit of 26 ppb and high CH<sub>3</sub>SH selectivity relative to (CH<sub>3</sub>)<sub>2</sub>S, H<sub>2</sub>S, CH<sub>3</sub>OH, C<sub>2</sub>H<sub>5</sub>OH, C<sub>3</sub>H<sub>6</sub>O, NH<sub>3</sub>, NO<sub>2</sub>, H<sub>2</sub>, CH<sub>4</sub>, C<sub>2</sub>H<sub>2</sub> and short-chain fatty acids (SCFAs) including CH<sub>3</sub>COOH, C<sub>2</sub>H<sub>5</sub>COOH, C<sub>3</sub>H<sub>7</sub>COOH. Therefore, the flame-synthesized Pt-PtO functionalized WO<sub>3</sub> nanosensors could be potential for CH<sub>3</sub>SH detection at ppb levels in the medical applications particularly noninvasive breath diagnosis of halitosis and serious infection of periodontitis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100464"},"PeriodicalIF":4.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of paralytic shellfish poisoning (PSP) toxins using a colorimetric immunosensing tool for food safety control 麻痹性贝类中毒（PSP）毒素的比色免疫感应检测用于食品安全控制

IF 4.1

Talanta Open Pub Date : 2025-04-27 DOI: 10.1016/j.talo.2025.100463

Ambbar Aballay-González , Sandra Leonardo , Margarita Fernández-Tejedor , Maria Rambla-Alegre , Jorge Diogène , AhlamAl Kharusi , Maximiliano Figueroa , Allisson Astuya-Villalón , Mònica Campàs

{"title":"Detection of paralytic shellfish poisoning (PSP) toxins using a colorimetric immunosensing tool for food safety control","authors":"Ambbar Aballay-González , Sandra Leonardo , Margarita Fernández-Tejedor , Maria Rambla-Alegre , Jorge Diogène , AhlamAl Kharusi , Maximiliano Figueroa , Allisson Astuya-Villalón , Mònica Campàs","doi":"10.1016/j.talo.2025.100463","DOIUrl":"10.1016/j.talo.2025.100463","url":null,"abstract":"<div><div>From 1985 to 2018, 35 % of shellfish poisoning cases worldwide were due to paralytic shellfish poisoning (PSP). The main representative toxin congener of the PSP toxin family is saxitoxin (STX), a highly toxic neurotoxin capable of blocking nerve impulse transmission and causing death by respiratory arrest, representing a danger to the environment and human health. The presence of PSP toxins in shellfish is currently detected through instrumental analysis methods, which are costly, or more rarely with the mouse bioassay, which is ethically criticized. Therefore, the development of new approaches for their detection is desired. This study presents the development of a colorimetric immunoassay in which STX has been immobilized on magnetic beads (MBs) for the subsequent competition between free STX present in the sample and immobilized STX for the binding site of an anti-STX antibody, using a secondary antibody labeled with horseradish peroxidase to reveal the signal. The immunoassay exhibits a dose-dependent behavior with an IC<sub>50</sub> for STX of 10.9 ng mL<sup>-1</sup>. The system shows low matrix effects with a loading capacity of 250 mg of shellfish flesh mL<sup>-1</sup> and, therefore, an effective limit of detection (LOD) of 17.2 µg kg⁻¹. The detection of STX and gonyautoxin-2 & gonyautoxin-3 in shellfish samples is also demonstrated. Furthermore, the covalent immobilization of STX on MBs is stable and allows the reusability of the system up to four times. This immunosensing system has been proved to be successful in the screening and quantification of PSP toxin contents in shellfish samples from Spain and Oman.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100463"},"PeriodicalIF":4.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid, highly specific and sensitive detection of human adenovirus using multiple cross displacement amplification coupled with CRISPR-Cas12a-based detection 利用多重交叉位移扩增和基于crispr - cas12的检测，快速、高特异性和敏感地检测人腺病毒

IF 4.1

Talanta Open Pub Date : 2025-04-27 DOI: 10.1016/j.talo.2025.100462

Linglong Wan , Juan Zhou , Lei Yu , Xiaolan Huang , Jin Fu , Fei Xiao , Nan Jia , Yu Zhang , Min Chen , Xu Chen , Zhaomin Feng , Yi Wang , Lihui Meng

{"title":"Rapid, highly specific and sensitive detection of human adenovirus using multiple cross displacement amplification coupled with CRISPR-Cas12a-based detection","authors":"Linglong Wan , Juan Zhou , Lei Yu , Xiaolan Huang , Jin Fu , Fei Xiao , Nan Jia , Yu Zhang , Min Chen , Xu Chen , Zhaomin Feng , Yi Wang , Lihui Meng","doi":"10.1016/j.talo.2025.100462","DOIUrl":"10.1016/j.talo.2025.100462","url":null,"abstract":"<div><div>Human adenoviruses (HAdV), particularly serotypes 3 and 7, are significant pathogens that cause respiratory tract infection. Here, we developed a novel HAdV diagnostic assay, termed HAdV-MCDA-CRISPR, by combining multiple cross displacement amplification (MCDA) with CRISPR/Cas12a-based detection. HAdV-MCDA-CRISPR assay uses MCDA to amplify target DNA, followed by detection of predefined sequences using a CRISPR/Cas12a-crRNA complex. Resulting fluorescence from probe cleavage indicates a positive result. A set of 10 MCDA primers and a specific crRNA were designed to ensure high specificity of HAdV-MCDA-CRISPR assay. The entire procedure, including rapid template preparation (∼15 min), MCDA reaction (40 min), and result interpretation (5 min), takes approximately one hour. Targeting the conserved hexon gene, HAdV-MCDA-CRISPR achieves high sensitivity under optimized MCDA reaction conditions (40 min at 66 °C), detecting as little as 1.92 copies/μL of hexon-containing plasmid DNA without cross-reactivity. Validation using 128 clinical samples demonstrated 78/80 positive results for HAdV-positive patients and 48/48 negative results for HAdV-negative patients. Thus, HAdV-MCDA-CRISPR assay offers a promising alternative for rapid and accurate clinical diagnosis of HAdV-3 and HAdV-7 infections.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100462"},"PeriodicalIF":4.1,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0