Talanta Open最新文献

{"title":"Greenness assessment profile of experimentally designed chromatographic method for the simultaneous analysis of ofloxacin and racecadotril in the presence of racecadotril impurities","authors":"Nada Nabil ,&nbsp;Ghada A. Sedik ,&nbsp;Hala E. Zaazaa ,&nbsp;Sally S. El-Mosallamy","doi":"10.1016/j.talo.2025.100487","DOIUrl":"10.1016/j.talo.2025.100487","url":null,"abstract":"<div><div>Experimental design beats the conventional one variable at a time (OVAT) to improve the chromatographic separations with lowest consumption of resources and maximum acceptable results for the separated peaks. Experimental design using face-centred composite design was utilized during method optimization to obtain the highest level of resolution with the fewest number of experimental trials for the determination of ofloxacin (OFLU) and racecadotril (RAC) in the presence of RAC impurities. The two independent variables were the pH of solvent A (0.10 % triethyl amine (pH 7 by orthophosphoric acid)) and its percentage in the mobile phase. RAC combined with OFLU is mainly used to treat diarrhoea in children. RAC has several impurities; among them is racecadotril impurity A (RAC-A) which was reported as a toxic impurity of RAC, additionally, RAC can be oxidized to racecadotril oxidative degradation product (RAC-Deg). Chromatographic separation was accomplished using Inertsil ODS column (150 mm, 4.6 mm, 5 μm) and isocratic elution using 0.10 % triethyl amine (pH 7 by orthophosphoric acid) (solvent A): methanol (solvent B) (20:80 v/v) as a mobile phase at a flow rate of 1.00 mL/min and UV detection at 240 nm. Sharp, well-resolved peaks for RAC-Deg, RAC-A, OFLU and RAC were obtained with retention times of 1.70, 2.06, 7.11, and 8.98 min, respectively. Method validation was carried out according to ICH guidelines and linearity was achieved in the ranges of 2.00–20.00 and 3.00–25.00 µg/mL for RAC and OFLU, respectively. The ecological impact of the presented technique was assessed utilizing the Analytical GREEnness Metric Approach and Software (AGREE) as an evaluation tool. The new approach shown high accuracy, selectivity, precision, and environmental friendliness in determining the mentioned drugs in pharmaceutical formulations. It is suitable for routine analysis of the drugs in quality control laboratories.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100487"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZnO-nanostructured electrochemical sensor for efficient detection of glyphosate in water","authors":"Zorica Novakovic ,&nbsp;Zorka Z. Vasiljevic ,&nbsp;Maria Vesna Nikolic ,&nbsp;Nenad B. Tadic ,&nbsp;Tijana Djordjevic ,&nbsp;Marko Radovic ,&nbsp;Ivana Gadjanski ,&nbsp;Snežana Papović ,&nbsp;Filip Vlahovic ,&nbsp;Dalibor Stankovic ,&nbsp;Jasmina Vidic","doi":"10.1016/j.talo.2025.100481","DOIUrl":"10.1016/j.talo.2025.100481","url":null,"abstract":"<div><div>Glyphosate is a widely used broad-spectrum herbicide for controlling grassy weeds, despite having potential health hazards. Herein, we report on a solid-state electrochemical sensor based on ZnO nanoparticles (ZnO NPs) for on-site detection of glyphosate. Accordingly, ZnO NPs was drop-cast on the surface of a disposable screen-printed carbon electrode. Eco-friendly ZnO NPs of only 7 nm crystallite sizes were obtained by green sol-gel synthesis using lemon (<em>Citrus limon</em>) waste aqueous extract as the green reducing and capping/stabilizing agent and Zn nitrate precursor as evidenced by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction and diffuse reflectance. SEM confirmed successful electrode functionalization with the synthesized nanoparticles. Under laboratory conditions in acetate buffer (pH 5), the sensor demonstrated excellent selectivity and sensitivity, with a detection limit of 0.648 µM, a wide linear detection range (0.5 µM to 7.5 mM), and a rapid detection time of 30 min. When tested in river water, the sensor achieved a detection limit of 0.96 µM using differential pulse voltammetry. It also exceptionally tolerated interference from similar organophosphorus compounds and ions commonly found in river water. The excellent detection performance of the sensor was attributed to the strong coordination interactions between Zn atoms and phosphonate/carboxylate groups that are enhanced by a hydrogen bond at acidic pH, as determined by chemical calculations. This disposable sensor offers a cost-effective, efficient, and environmentally friendly solution for monitoring glyphosate in water systems.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100481"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of molecularly imprinted polymers on graphene oxide-coated screen-printed electrodes for selective immune-sensing of gastric cancer-causing bacteria Helicobacter pylori","authors":"Akshita ,&nbsp;Warren Rosario ,&nbsp;Nidhi Chauhan ,&nbsp;Utkarsh Jain","doi":"10.1016/j.talo.2025.100489","DOIUrl":"10.1016/j.talo.2025.100489","url":null,"abstract":"<div><div><em>Helicobacter pylori (H. pylori)</em> infection continues to be a major public health issue worldwide. The prevalence of <em>H. pylori</em> infection exceeds 50 % worldwide and reaches 70–80 % in developing countries because of inadequate sanitation and restricted healthcare services. The gram-negative bacterium <em>H. pylori</em> hold Group 1 carcinogen status according to the World Health Organization (WHO) because it directly causes chronic gastritis, mucosa-associated lymphoid tissue (MALT), peptic ulcer disease, and gastric adenocarcinoma. This calls for an immediate need for better diagnostic approaches. The gastric mucosa colonization of the bacterium depends on outer membrane proteins, including BabA, OipA, and SabA, which facilitate both adhesion and pathogenicity. The current diagnostic approaches, including biopsy, histology, endoscopy, fecal antigen tests, ELISA, serology, western blotting, urea breath tests, and PCR, face challenges related to complexity, high costs, and low sensitivity. The development of electrochemical biosensors represents a promising diagnostic solution because they provide fast results at affordable prices and portable devices for on-site testing. The incorporation of molecularly imprinted polymers (MIPs) as synthetic receptors with predefined recognition sites for target analytes improves both selectivity and stability of these biosensing platforms. The research introduces an innovative electrochemical biosensor system that detects BabA antibodies (BabA-Ab), which serve as important immunological indicators of <em>H. pylori</em> infection. The sensor platform consists of graphene oxide (GO) that integrates with a BabA-Ab-imprinted polymer matrix. A non-imprinted polymer (NIP) sensor device was constructed simultaneously with the BabA-Ab-imprinted polymer sensor to function as a control reference. The research demonstrates how GO/MIP-based electrochemical sensors can function as advanced clinical diagnostic instruments for fast and precise medical testing.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100489"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144213325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review on the adsorptive removal of pharmaceutical pollutants: Occurrence, toxicology, molecular simulation and mechanistic insights","authors":"Mochamad Lutfi Firmansyah ,&nbsp;Yousef Alwan ,&nbsp;Nisar Ullah","doi":"10.1016/j.talo.2025.100491","DOIUrl":"10.1016/j.talo.2025.100491","url":null,"abstract":"<div><div>Amid rapid industrialization and soaring population growth, there is an ever-increasing global use of medicines that has grown by 14 % over the past five years. The excessive use of pharmaceuticals causes contamination of water bodies through domestic and hospital effluents, as well as by pharmaceutical companies. This in turn threatens global drinking water supplies. Moreover, the biologically active nature of pharmaceutical pollutants (PPs) makes it difficult to degrade in wastewater and hence poses notable risks to both public health and as well as the environment. Given the growing interest in the removal of PPs from wastewater, adsorption is regarded as a simple yet highly effective strategy. In this review, we catalogue recent progress in the adsorptive removal of PPs from wastewater, covering the period from 2013 to the present date. We have systematically covered the comparative overview of existing removal approaches, followed by a detailed examination of various adsorbent materials. Moreover, the influence of porosity, surface area, and functional groups on the adsorption efficiency has been discussed. Likewise, critical considerations, such as optimizing maximum adsorption capacity, the effect of pH, adsorption mechanisms, and adsorbent regeneration, have been analyzed. Moreover, computational and machine-learning–guided molecular design methods in the design of the adsorbent has also been reviewed. Furthermore, the toxicity and hazardous effects of PPs on human health, aquatic ecosystems, and the environment have been extensively discussed. Finally, we outline the disadvantages of the existing adsorbents, elaboration on the research gaps and possible solutions, with the aim to develop more efficient and cost-effective adsorbents in the quest to remove PPs from wastewater.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100491"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterostructured Zn-2MI@V-COF-1@MWCNTs based electrochemical sensor for the sensitive simultaneous detection of ethyl paraben, diclofenac and epinephrine","authors":"Antonia Trisha Zac R,&nbsp;Yao-An Tsai,&nbsp;Chih-Ling Yeh,&nbsp;Hui-Ling Lee","doi":"10.1016/j.talo.2025.100488","DOIUrl":"10.1016/j.talo.2025.100488","url":null,"abstract":"<div><div>Rational designing of practical and cost-effective electrochemical sensor with simultaneous sensing of ethyl paraben (EPB), diclofenac (DIC), and epinephrine (EPN) remains a challenging aspect. With a motive to solve this concern, a low-cost screen printed electrodes (SPCE) sensor with a novel hybrid Zn-2MI@V-COF-1@MWCNTs modifications was put forward. In this work, an octahedral imidazole based metal organic frameworks (Zn-2MI) is combined with a two-dimensional vinylene based covalent organic frameworks (V-COF-1) to form (Zn-MOF@V-COF-1) through a simple room temperature synthesis. This hybrid is further integrated with multi-walled carbon nanotubes (MWCNTs) for enhanced analyte detection. The structural and crystalline nature of the hybrid materials was analysed using PXRD, SEM, and TEM. The material exhibits ideal porosity and a large electroactive area with multiple adsorption sites, resulting in effective, sensitive detection towards EPN, DIC, and EPB. The sensor achieves low detection limits of 0.05 µM for DIC and EPN and 0.5 µM for EPB. Real samples analysis including chili powder, liquid sweetener and energy drink are conducted using differential pulse voltammetry using satisfactory results ranging recovery rates from 81.54 to 105.8 %. These excellent performance outcomes, attributes to the synergistic effect and the presence of multiple active sites elucidates that novel Zn-2MI@V-COF-1@MWCNTs/SPCE are reliable sensors that offers quick screening, cost effective, sensitive, and allow simultaneous detection of harmful analytes in complex matrices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100488"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfur-nitrogen doped carbon dots for Bi³⁺ ion detection and temperature sensing","authors":"Zhanbiao He ,&nbsp;Zhen Wang ,&nbsp;Lu Ga ,&nbsp;Jun Ai ,&nbsp;Pingping Liu","doi":"10.1016/j.talo.2025.100482","DOIUrl":"10.1016/j.talo.2025.100482","url":null,"abstract":"<div><div>In this work, sulfur-nitrogen doped carbon dots (N,S-CDs) were synthesized through a facile one-step hydrothermal approach using 2-Mercaptopyrimidine and citric acid as precursors. The as-prepared N,S-CDs exhibited a remarkable fluorescence response towards Bi³⁺ ions, achieving highly sensitive detection in the concentration range of 0–45 μM with a detection limit of 230 nM. These findings suggest great potential for the application of N,S-CDs in the detection of real water samples. Furthermore, the N,S-CDs demonstrated an excellent temperature response, showing reversible and recoverable fluorescence characteristics within the temperature range of 25 to 65 °C. The low cytotoxicity and in vitro cellular imaging properties displayed by this carbon dot further contribute to its enhanced functionality and practicality.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100482"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144148047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotinylated fusion protein-streptavidin-peroxidase complex based non-competitive magnetic immunoassay for rapid detection of organophosphorus pesticides","authors":"Ruirui Shi ,&nbsp;Jiangke Li ,&nbsp;Wenli Yu ,&nbsp;Tengfei Yin ,&nbsp;Haiyan Lin ,&nbsp;Juan Liu ,&nbsp;Wenyu Cui","doi":"10.1016/j.talo.2025.100483","DOIUrl":"10.1016/j.talo.2025.100483","url":null,"abstract":"<div><div>Phage-displayed anti-immunocomplex peptide-based non-competitive immunoassays have shown great potential for detecting organophosphorus pesticide (OPs) residues. However, the phage-borne peptides raise safety concerns and require secondary reagents, which limit their commercial application. To address these challenges, a new approach has been developed. This study employed the SIC2 peptide, which recognized the immune complex of single-chain variable fragment 5 (scFv5) and OPs, to reveal its potential for detecting OPs residues. The SIC2 peptide was fused with glutathione S-transferase (GST) and biotin acceptor domain (BAD) genes. These fusion constructs were expressed in <em>Escherichia coli</em> BL21 (DE3) and then biotinylated. The biotinylated fusion protein bound to streptavidin-horseradish peroxidase (SA-HRP) conjugates through the biotin-streptavidin system, enhancing the detection signal via the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Based on this, and using immunomagnetic beads, a rapid non-competitive magnetic immunoassay (NCMIA) was developed for detecting OPs. The results showed that under optimal conditions, the limits of detection (LODs) for 12 OPs ranging from 1.04 to 13.18 ng mL^-1, and the incubation time of this assay needed 11 min. The intra- and inter-batch recovery rates of 3 OPs in cucumber, cabbage and lettuce ranged from 79.91 % to 103.72 %, with coefficients of variation ranging from 0.26 % to 14.98 %. In addition, the recoveries of parathion determined by NCMIA were consistent with the results of parallel analyses using gas chromatography tandem mass spectrometry (GC–MS/MS).</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100483"},"PeriodicalIF":4.1,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic 3D printable electrochemical filament integrated with CuO nanopowder for enzyme-free biosensing","authors":"Leonidas Papagiannakopoulos ,&nbsp;Vasiliki Polyzopoulou ,&nbsp;Loizos Tsolakis ,&nbsp;Eleni Sorkou,&nbsp;Eleni Koukouviti,&nbsp;Anastasios Economou,&nbsp;Christos Kokkinos","doi":"10.1016/j.talo.2025.100480","DOIUrl":"10.1016/j.talo.2025.100480","url":null,"abstract":"<div><div>3D printing technology enables the on-demand fabrication of low-cost thermoplastic electrodes, which have shown promising results in enzymatic bioassays. To fully harness the potential of 3D printing in electrochemical biosensing, this work introduces a new generation of tailor-made conductive filament integrated with a biomimetic functional material for the 3D printing of ready-to-use sensors designed for enzyme-free biodeterminations. The filament was synthesized using the solvent casting method and was composed of polylactic acid as the base, carbon black as the conductive filler, and CuO nanopowder as the artificial nanozyme. The filament was characterized using thermogravimetry, energy-dispersive X-ray spectroscopy, scanning electron microscopy, and electrochemical techniques. The as-printed sensors enabled direct electrochemical monitoring of crucial bioindicators—glucose (GLU), lactic acid (LA), and creatinine (CRE)—with exceptional sensitivity, offering limits of detection of 5.1 µM, 0.12 mM, and 1.5 µM, respectively. Moreover, the sensors exhibited high anti-interference capability and were successfully applied to the determination of the target biomarkers in blood, sweat, and urine samples. The amperometric determination of GLU was based on the conversion of integrated CuO to CuOOH, which subsequently oxidized GLU to gluconic acid. The voltammetric determination of CRE was based on the formation of copper-creatinine complexes, resulting in the suppression of the oxidation signal of electrogenerated copper. Meanwhile, LA voltammetric detection relied on the characteristic increases in the redox signals of CuO. This work paves the way for the development of accessible, point-of-need printable, and ready-to-use electrochemical biomimetic sensors for easy applications in the biosensing field.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100480"},"PeriodicalIF":4.1,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel and Green UFLC-MS/MS Method for Quantification of Amantadine and Levodopa in Polymeric Nanoparticles: Application to determine Drug loading (%DL), Drug entrapment (%DEE) and Drug release profile","authors":"Akanksha Lahiri ,&nbsp;Balamuralidhara V ,&nbsp;Hemanth Vikram PR ,&nbsp;Karthika Paul","doi":"10.1016/j.talo.2025.100476","DOIUrl":"10.1016/j.talo.2025.100476","url":null,"abstract":"<div><h3>Introduction</h3><div>Levodopa, a dopamine precursor, widely prescribed drug in Parkinson’s disease management possesses a side effect of Levodopa-induced dyskinesia (LID). Amantadine hydrochloride, an NMDA receptor antagonist is co-encapsulated along with levodopa and formulates as polymeric nanoparticles (NPs) so as to overcome the side effects and act synergistically to enhance therapeutic outcome.</div></div><div><h3>Methodology</h3><div>In current work, we developed a novel, green Ultra-Fast Liquid Chromatography-Tandem Mass Spectrometry (UFLC-MS/MS) technique for the simultaneous quantification of amantadine and levodopa in polymeric nanoparticles. A triple quadrupole analyser with a multiple reaction monitoring (MRM) scan mode and an atmospheric pressure chemical ionization (APCI) source. A Waters Symmetry C8 column (150 × 4.6 mm, 3.5 μm) maintained at 40 °C was used for the chromatographic separation. In order to ensure sensitive and specific analyte detection, the mobile phase consisted of 0.1 % formic acid in water and methanol (40:60) with a total run time of 5 min. Excellent linearity, recovery, accuracy, and sensitivity were validated by method validation. Greenness assessment was done by AGREE, GAPI and AES metrics.</div></div><div><h3>Results</h3><div>Proposed green UFLC-MS/MS method effectively quantifies Levodopa and Amantadine in polymeric nanoparticles, followed by accurate evaluation of %DL, %DEE, and drug release profiles. %DEE values were observed for Levodopa (89.6%) and Amantadine (90.16 %), with corresponding %DL values of 20.5% and 24.10%, indicating substantial drug loading capacity. The in vitro drug release profiles demonstrated sustained release behaviour, with 91.89% ± 0.362 of Levodopa and 92% ± 0.362 of Amantadine released over the study period.</div></div><div><h3>Conclusion</h3><div>Using GAPI, AGREE, and AES criteria, the created analytical method's greenness was carefully assessed. The outcomes were then compared to previously published methods in the literature. For the simultaneous measurement of amantadine and levodopa in nanoparticles, our innovative UFLC-MS/MS technology offered a dependable and extremely sensitive method.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100476"},"PeriodicalIF":4.1,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of activated graphene based electrodes for ultrasensitive simultaneous electrochemical detection of uric acid and dopamine","authors":"Yogalakshmi Vadivelu ,&nbsp;Aishwarya Sendrayal Raj ,&nbsp;Rajkumar Muniyandi ,&nbsp;Srither S．R． ,&nbsp;Balaji Ramachandran","doi":"10.1016/j.talo.2025.100477","DOIUrl":"10.1016/j.talo.2025.100477","url":null,"abstract":"<div><div>We describe a facile and efficient chemical activation technique for the synthesis of activated graphene-modified electrodes able to perform simultaneous and specific identification of uric acid (UA) and dopamine (DA) biomolecules. To find out suitable agent for this specific electrocatalytic application, we have used three chemicals, namely potassium hydroxide, zinc chloride, and phosphoric acid that have been tested. Cyclic voltammogram (CV), linear sweep voltammogram (LSV), differential pulse voltammogram (DPV), electrochemical impedance spectroscopy (EIS) measurements, field emission scanning electrochemical microscopy (FESEM), and transmission electron microscopy (TEM) techniques are performed to analyze the activated graphene-modified electrodes being utilized as an electrochemical sensor, it can effectively detect dopamine and uric acid simultaneously in the existence of ascorbic acid. The KOH-activated graphene (AGK) exhibits a high electrocatalytic activity towards selectively detecting dopamine and uric acid. In the DPV and LSV studies, the peak gap between dopamine (DA) and uric acid (UA) reaches a maximum of 0.23 V. The calibration curves were calculated, with detection limits of 1.96 µM for DA and 1.97 µM for UA in LSV, and 0.09 µM for DA and 0.19 µM for UA in DPV, respectively. The ranges of the calibration curves are 2–380 µM for LSV and 0.02–400 µM for DPV. The presented AGK-modified screen-printed carbon electrode has been effectively operated to efficiently detect DA and UA selectively and simultaneously in real-time samples performed with acceptable results.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100477"},"PeriodicalIF":4.1,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}