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An analytical method to quantify tetrabromobisphenol A (TBBPA) and its conjugated metabolites in rat serum or other biological samples 一种定量大鼠血清或其它生物样品中四溴双酚A (TBBPA)及其共轭代谢物的分析方法
IF 3.7
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-08-23 DOI: 10.1016/j.talo.2025.100537
Mette Stub, Mikael Pedersen, Louise Ramhøj, Kit Granby, Terje Svingen, Agnieszka Anna Niklas
{"title":"An analytical method to quantify tetrabromobisphenol A (TBBPA) and its conjugated metabolites in rat serum or other biological samples","authors":"Mette Stub,&nbsp;Mikael Pedersen,&nbsp;Louise Ramhøj,&nbsp;Kit Granby,&nbsp;Terje Svingen,&nbsp;Agnieszka Anna Niklas","doi":"10.1016/j.talo.2025.100537","DOIUrl":"10.1016/j.talo.2025.100537","url":null,"abstract":"<div><div>Brominated flame retardants (BFRs) are widely used to enhance fire resistance in consumer and commercial products, but often leach into the environment due to their surface application. Tetrabromobisphenol A (TBBPA), the most applied BFR, has been detected in human serum, urine, and breast milk. TBBPA is readily absorbed from the gastrointestinal tract, undergoing first-pass hepatic metabolism to form the two major metabolites TBBPA-sulfate and TBBPA-glucuronide. Despite evidence linking TBBPA exposure to developmental toxicity, neurobehavioral effects, uterine cancers, and endocrine disrupting potential via thyroid hormone interference, exposure assessments remain challenging. Current methods rely heavily on direct quantification of TBBPA and its metabolites using liquid chromatography-mass spectrometry (LC-MS), but a lack of commercially available TBBPA metabolites at analytical standards is a limiting factor. This study introduces a method to rapidly quantify TBBPA, and after enzymatic deconjugation, its metabolites using LC-Orbitrap. Using this method, TBBPA-glucuronide was the only metabolite detected in rat serum following oral exposure. Native TBBPA was obtained using enzymatic deconjugation of serum. The protocol simplifies exposure evaluation by offering a practical, and rapid, approach for quantifying TBBPA in biological samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100537"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Analytical methodologies for antioxidant capacity assessment: An updated review 抗氧化能力评估的分析方法:最新综述
IF 3.7
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-09-14 DOI: 10.1016/j.talo.2025.100558
Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour
{"title":"Analytical methodologies for antioxidant capacity assessment: An updated review","authors":"Mahmoud Hamed ,&nbsp;Faisal K. Algethami ,&nbsp;Alaa Bedair ,&nbsp;Fotouh R. Mansour","doi":"10.1016/j.talo.2025.100558","DOIUrl":"10.1016/j.talo.2025.100558","url":null,"abstract":"<div><div>Antioxidants are crucial molecules protecting biological systems from harmful oxidation reactions and free radicals. This class of compounds attracted remarkable attention for their potential in functional foods and dietary supplements. The imbalance between oxidants and antioxidants leads to oxidative stress, associated with various chronic diseases. Accurate measurement of antioxidant activity is therefore essential for evaluating potential health-enhancing agents in food science, medicine, and biotechnology. This review provides a comprehensive overview of contemporary analytical methodologies for assessing antioxidant capacity across food, cosmetic, pharmaceutical, and biomedical applications, exploring four major categories of techniques: spectrophotometric methods, including UV–Vis, electron paramagnetic resonance (EPR), and near infrared (NIR) spectroscopy, highlighting their principles and applications in radical scavenging assays and antioxidant characterization. Electrochemical techniques are rapid, sensitive, and cost-effective alternatives, focusing on voltammetry, amperometry, and biosensor development for direct measurement of electron transfer processes. The role of biosensors, employing bioreceptors like enzymes and DNA, for selective and sensitive antioxidant detection is also examined. Chromatographic methods, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), paired with selective detectors, are used to separate, identify, and quantify antioxidant compounds in complex matrices. This review highlights the principles, advancements, advantages, and limitations of these diverse methodologies in the accurate evaluation of antioxidant potential.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100558"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers 一种简单、高通量ICP-MS测定尿液中46种元素的方法:汽车工人职业暴露的生物监测
IF 3.7
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-09-14 DOI: 10.1016/j.talo.2025.100559
Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen
{"title":"A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers","authors":"Ruirui Dai ,&nbsp;Huadong Zhang ,&nbsp;Wei Yang ,&nbsp;Xiaoxi Wang ,&nbsp;Xiaoping Li ,&nbsp;Yujie Yu ,&nbsp;Fengqiong Chen","doi":"10.1016/j.talo.2025.100559","DOIUrl":"10.1016/j.talo.2025.100559","url":null,"abstract":"<div><div>Monitoring urinary metal concentrations is crucial for assessing occupational exposure and associated health risks. Inductively coupled plasma mass spectrometry (ICP-MS) is widely considered the method of choice for multi-element analysis due to its high sensitivity, low detection limits, and broad dynamic range. This study presents a simple and rapid ICP-MS method for the simultaneous determination of 46 elements in urine, utilizing 2 % nitric acid and 1.5 % ethanol (v/v) for sample pretreatment. This approach effectively mitigates matrix effects and carbon-based interferences. The method demonstrated excellent linearity (coefficient of determination, R² ≥ 0.9985), precision (relative standard deviation, RSD &lt; 8 %), and accuracy (recoveries 80–120 %). The method was applied to analyze 268 urine samples from automotive manufacturing workers. Several elements of occupational relevance, including Nickel (Ni), tungsten (W), molybdenum (Mo), titanium (Ti) and Cadmium (Cd), as well as essential elements such as iron (Fe), copper (Cu), and zinc (Zn), were elevated compared with general population references, indicating complex multi-metal exposure profiles. This work provides a validated and efficient tool for occupational biomonitoring and delivers valuable elementomic data to support exposure assessment, workplace interventions, and future research on the health effects of multi-metal exposure.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100559"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging 同时检测H2S和黏度的比例近红外荧光探针:在环境监测和生物成像中的应用
IF 3.7
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-09-23 DOI: 10.1016/j.talo.2025.100564
Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren
{"title":"A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging","authors":"Sha Li,&nbsp;Qin Li,&nbsp;Zhihui Yang,&nbsp;Wei Wen,&nbsp;Miao Yan,&nbsp;Haixian Ren","doi":"10.1016/j.talo.2025.100564","DOIUrl":"10.1016/j.talo.2025.100564","url":null,"abstract":"<div><div>Hydrogen sulfide (H<sub>2</sub>S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, <strong>T-N</strong>, enabling simultaneous and discriminative detection of H<sub>2</sub>S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (&lt;30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H<sub>2</sub>S-triggered chromogenic shifts, <strong>T-N</strong>-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, <strong>T-N</strong> achieves synchronous dual-channel imaging of endogenous/exogenous H<sub>2</sub>S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H<sub>2</sub>S and viscosity in interconnected environmental, food safety, and biomedical contexts.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100564"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-performance MIL-53(Fe)-incorporated cellulose acetate membranes for efficient dye and wastewater treatment 高性能MIL-53(Fe)-醋酸纤维素膜,用于高效染料和废水处理
IF 4.1
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-07-14 DOI: 10.1016/j.talo.2025.100517
Suvarna P. Dhongade , Surya Teja Malkapuram , Shirish H. Sonawane , Sivakumar Manickam
{"title":"High-performance MIL-53(Fe)-incorporated cellulose acetate membranes for efficient dye and wastewater treatment","authors":"Suvarna P. Dhongade ,&nbsp;Surya Teja Malkapuram ,&nbsp;Shirish H. Sonawane ,&nbsp;Sivakumar Manickam","doi":"10.1016/j.talo.2025.100517","DOIUrl":"10.1016/j.talo.2025.100517","url":null,"abstract":"<div><div>This study reports the development of high-performance cellulose acetate (CA) membranes embedded with MIL-53(Fe), a metal organic framework (MOF), for efficient dye removal from wastewater. MIL-53(Fe) was synthesized via solvothermal methods and comprehensively characterized using XRD, FTIR, FESEM, TEM, BET, and TGA analyses. XRD and FTIR confirmed the successful formation and integration of MIL-53(Fe) within the CA matrix, while BET analysis revealed a surface area of 34.16 m<sup>2</sup>/g and pore diameter of 3.42 nm. Electron microscopy demonstrated well-defined polyhedral morphologies and layered crystalline domains, with AFM revealing an increase in surface roughness as MOF loading increased. Composite membranes exhibited enhanced thermal stability and mechanical strength, with tensile strength improving by ∼75 % at 0.75 wt.% MOF due to optimal dispersion; higher loadings led to agglomeration and performance decline. The CA/MIL-0.75 membrane achieved superior water permeability and dye rejection, removing 96 % of Safranin-O and 98 % of Direct Yellow-12. Surface densification, improved porosity, and increased hydrophilicity contributed to enhanced separation performance. Real wastewater treatment trials confirmed effective pollutant removal from textile effluent and greywater. These findings underscore the potential of MIL-53(Fe)-modified CA membranes as robust, thermally stable, and efficient materials for sustainable water purification applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100517"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144666052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Recent advancements in microcystin detection: A comprehensive review of immunosensors and nucleic acid-based biosensors for efficient monitoring in drinking water and human samples 微囊藻毒素检测的最新进展:用于饮用水和人体样本有效监测的免疫传感器和基于核酸的生物传感器的综合综述
IF 4.1
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-05-29 DOI: 10.1016/j.talo.2025.100495
Anahas Perianaika Matharasi Antonyraj , Prasannabalaji Nainangu , Ayyakannu Sundaram Ganeshraja , Ajay Guru , Kumaran Subramanian , Manju Dhakad Tanwar , Vairavel Parimelazhagan , A. Santhana Krishna Kumar
{"title":"Recent advancements in microcystin detection: A comprehensive review of immunosensors and nucleic acid-based biosensors for efficient monitoring in drinking water and human samples","authors":"Anahas Perianaika Matharasi Antonyraj ,&nbsp;Prasannabalaji Nainangu ,&nbsp;Ayyakannu Sundaram Ganeshraja ,&nbsp;Ajay Guru ,&nbsp;Kumaran Subramanian ,&nbsp;Manju Dhakad Tanwar ,&nbsp;Vairavel Parimelazhagan ,&nbsp;A. Santhana Krishna Kumar","doi":"10.1016/j.talo.2025.100495","DOIUrl":"10.1016/j.talo.2025.100495","url":null,"abstract":"<div><div>Cyanobacteria produce harmful peptides called microcystins (MCs), which are a major concern for the public's health and the water supply. The safety of the water supply depends on the accurate identification of these toxins. A state-of-the-art immunosensor that immobilizes MC variants targeted by monoclonal antibodies onto nanostructured substrates allows for fast and selective detection of MC, as discussed in this review article. Incorporating nanomaterials such as gold nanoparticles and carbon nanotubes, the detector improves the effectiveness of the signal, allowing it to identify as low as 0.05 ppb with a response time of &lt;10 min. With its stable performance over time, this type of sensor is excellent for field deployment and ongoing surveillance. Miniaturizing the device, improving its multifaceted tracking capabilities, and testing it in real-world events are the main goals of future research. The integration of novel technologies and nanomaterials is highlighted in the review as an approach to enhance sensitivity, accessibility, and exchange of data in real-time. Miniaturized portable devices, enhancement of signals, and connection with the Internet of Things are highlighted as significant innovations in environmental monitoring and public health protection. To protect ecosystems and public health, next-generation biosensing technologies could decrease MC contamination in water bodies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100495"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144242730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Design and synthesis of 8-nitroquinoline azine D-π-A module chemosensors: Fluorogenic onsite detection of sarin gas mimic- DCP 8-硝基喹啉嗪D-π- a模块化学传感器的设计与合成:沙林气体模拟物- DCP的荧光现场检测
IF 4.1
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-06-29 DOI: 10.1016/j.talo.2025.100508
Sentamil Selvi Ramasamy , Kaviyarasu Adhigaman , Vandana Nandakumar , Akilesh Muralidharan , Shankar Ramasamy , Suresh Thangaraj
{"title":"Design and synthesis of 8-nitroquinoline azine D-π-A module chemosensors: Fluorogenic onsite detection of sarin gas mimic- DCP","authors":"Sentamil Selvi Ramasamy ,&nbsp;Kaviyarasu Adhigaman ,&nbsp;Vandana Nandakumar ,&nbsp;Akilesh Muralidharan ,&nbsp;Shankar Ramasamy ,&nbsp;Suresh Thangaraj","doi":"10.1016/j.talo.2025.100508","DOIUrl":"10.1016/j.talo.2025.100508","url":null,"abstract":"<div><div>Organophosphorus (OP) chemical warfare agents are highly toxic and directly interrupts the acetylcholine esterase (AChE) enzyme activity in the central nervous system while exposed to those lethal substances. Detection of OP agents is challenging one and existing analytical methods are also having limitations and tedious operating procedures. In this context, we designed two ICT based 8-nitro substituted quinoline unsymmetrical azine sensors (<strong>NQH</strong> and <strong>NQH-1)</strong> for the detection of sarin mimic diethylchlorophosphate (DCP) nerve agent. The developed sensors are shown significant orange region emissive responses to DCP molecule in “TURN ON” manner with reliable red shifts. The limit of detection for the probes <strong>NQH</strong> and <strong>NQH-1</strong> were in micromolar level found to be 1.5 and 3.2 µM respectively. The consequent mechanistic pathway proposed for the sensing systems are PET combined with ICT for <strong>NQH</strong>, while the <strong>NQH-1</strong> system functions through ESIPT coupled with ICT processes. The proposed mechanistic gateway further elucidated through <sup>1</sup>H NMR, <sup>31</sup>P NMR titrations in addition to the dynamics of DFT. Notably, the proposed <span>D</span>-π-A module chemosensors have good fluorescent module for instant field level recognition of nerve agents (surrogates). The significance of the research found in the sensing platform has been achieved by the fabrication of cotton swab and paper disc kits for the real time analysis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100508"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
High-performance ratiometric optical oxygen sensor fabricated via 3Dprinted silicone for biomedical applications 用于生物医学应用的高性能比例光学氧传感器由3d打印硅胶制成
IF 4.1
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-07-05 DOI: 10.1016/j.talo.2025.100513
Rispandi , Cheng-Shane Chu , Abdulfatah Abdu Yusuf , Muhammad Imam Ammarullah
{"title":"High-performance ratiometric optical oxygen sensor fabricated via 3Dprinted silicone for biomedical applications","authors":"Rispandi ,&nbsp;Cheng-Shane Chu ,&nbsp;Abdulfatah Abdu Yusuf ,&nbsp;Muhammad Imam Ammarullah","doi":"10.1016/j.talo.2025.100513","DOIUrl":"10.1016/j.talo.2025.100513","url":null,"abstract":"<div><div>A new design of ratiometric optical oxygen sensors demonstrates considerable potential for applications across medical, environmental, and industrial fields. These sensors are recognized for their high sensitivity, specificity, and resistance to interference from external environmental variables. This research introduces an innovative fabrication method using silicone-based 3D printing technology to develop such sensors. The approach integrates a silicone matrix with oxygen-sensitive dyes, specifically platinum(II) meso‑tetrakis(pentafluorophenyl)porphyrin (PtTFPP) or tris(4,7-diphenyl-1,10-phenanthroline), Ru(dpp)₃²⁺, along with reference dyes such as Rhodamine 110 or 7-amino-4-trifluoromethyl coumarin, which are unaffected by oxygen. These phosphorescent compounds are blended into the silicone medium by manual stirring. Using a 405 nm LED as the excitation source, it was verified that the emission spectra of the sensing and reference dyes do not overlap, allowing reliable oxygen detection via a ratiometric method. The effectiveness of the sensors is evaluated by comparing the intensity ratio <em>I<sub>N₂/</sub>I<sub>O₂</sub></em>, where <em>I<sub>N₂</sub></em> and <em>IO₂</em> correspond to the luminescent outputs in nitrogen and oxygen atmospheres, respectively. The sensors embedded with PtTFPP and Ru(dpp)₃²⁺ show linear Stern–Volmer behavior, with sensitivity values of about 69 and 12, respectively. Furthermore, the PtTFPP-based sensor exhibits response times of 41 s and 64 s for oxygen increase and decrease transitions, respectively, while the Ru(dpp)₃²⁺-based sensor records 45 and 68 s for the same changes. This ratiometric sensing approach enhances detection sensitivity and response consistency, minimizing variations caused by fluctuations in light source intensity or fiber optics. Combining ratiometric oxygen sensing with 3D-printed silicone structures provides a robust foundation for developing next-generation oxygen sensor technologies with improved capabilities. The silicone matrix's biocompatible nature and the sensor components' high performance are a significant step toward integrating additive manufacturing and optical sensing in developing reliable, miniaturized biomedical sensors for future healthcare technologies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100513"},"PeriodicalIF":4.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144611725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness 环保高效液相色谱法同时测定泮托拉唑和多潘立酮的绿、白、蓝综合评价
IF 3.7
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-09-15 DOI: 10.1016/j.talo.2025.100560
Aya Barseem , Reem H. Obaydo , Samar H. Elagamy
{"title":"Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness","authors":"Aya Barseem ,&nbsp;Reem H. Obaydo ,&nbsp;Samar H. Elagamy","doi":"10.1016/j.talo.2025.100560","DOIUrl":"10.1016/j.talo.2025.100560","url":null,"abstract":"<div><div>An environmentally friendly RP-HPLC method was developed for the simultaneous determination of pantoprazole (PAN) and domperidone (DOM). Chromatographic separation was performed using isocratic elution on CORTECS® Shield RP18 column maintained at 25 °C. The mobile phase consisted of ethanol and potassium phosphate buffer (50.0 mM, pH 6.0) in a 30:70 (v/v) ratio, delivered at a flow rate of 1.0 mL/min, with UV detection at 286 nm. The method demonstrated excellent linearity within the concentration ranges of 1.0–25.0 μg/mL for PAN and 3.0–30.0 μg/mL for DOM. It was successfully applied to the analysis of both drugs in bulk and pharmaceutical dosage forms. The greenness of the proposed method was evaluated using the Analytical Eco-Scale, yielding a total score of 83, which indicates minimal environmental impact. The method \"whiteness\" was assessed using the RGB 12 model, resulting in a high total score of 89.2, reflecting a well-balanced combination of analytical performance, environmental sustainability, and practical implementation. Its \"blueness,\" representing practical applicability, was measured using the BAGI tool and achieved a total score of 82.5. Compared to a previously reported HPLC method, the proposed method offers comparable practical applicability while exhibiting enhanced greenness through the use of ethanol as the organic modifier, rather than environmentally hazardous solvents such as acetonitrile and methanol. The method was fully validated according to ICH Q2(R1) guidelines, confirming its accuracy, precision, specificity, and robustness. Furthermore, the proposed method was statistically compared with a previously reported HPLC method using Student’s <em>t</em>-test and F-test.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100560"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Green rapid HPLC method for testing retinol and tocopherol in ophthalmic gels 绿色快速高效液相色谱法测定眼用凝胶中视黄醇和生育酚
IF 3.7
Talanta Open Pub Date : 2025-12-01 Epub Date: 2025-08-28 DOI: 10.1016/j.talo.2025.100538
Xiao-Chen Huang , Ahmed Sobhy Darwish , Wageh Sobhy Darwish , Ri-Meng Chen , Jin-Kui Ma
{"title":"Green rapid HPLC method for testing retinol and tocopherol in ophthalmic gels","authors":"Xiao-Chen Huang ,&nbsp;Ahmed Sobhy Darwish ,&nbsp;Wageh Sobhy Darwish ,&nbsp;Ri-Meng Chen ,&nbsp;Jin-Kui Ma","doi":"10.1016/j.talo.2025.100538","DOIUrl":"10.1016/j.talo.2025.100538","url":null,"abstract":"<div><div>In addition to providing immediate comfort for dry and irritated eyes, vitamin A (retinol) and E (tocopherol) drops strengthen the eyes' defenses. Optic gels, which are compatible with contact lenses, use vitamins A and E to improve vision, soothe and hydrate dry and sensitive eyes, and provide immediate comfort. It is now feasible to determine the amounts of vitamin A and vitamin E in ocular gels thanks to the development and validation of a thorough, rapid, simple, and eco-friendly stability-indicating LC approach. In compliance with ICH guidelines, the two medications underwent a series of assessments. Vitamin A palmitate, vitamin E acetate, and related degradants were successfully resolved via isocratic separation. Utilizing the octadecylsilyl phase at ambient temperature (25° <em>C</em> ± 5°C), both drugs were quantified and qualified. Constant ratios of filtered water (neutral purified water at pH range 5 to 7) to ethanol comprise the isocratic mobile phase, which has a flow rate of 2.0 mL/min. During the 290 nm detection, the concentrations of vitamin A palmitate and vitamin E acetate varied from 5 to 200 IU/mL and 25 to 1000 μg/mL, respectively. The LC technique's ecological benefits are demonstrated by its overall AES grade of 79, AGREE grade of 0.64, MoGAPI grade of 76, BAGI grade of 80, and final whiteness of 95.8. According to research, the suggested method is accurate, reliable, long-lasting, eco-friendly, and distinctive.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100538"},"PeriodicalIF":3.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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