Talanta Open最新文献

{"title":"Radiation evaluation assay using a human three-dimensional oral cancer model for clinical radiation therapy.","authors":"Lucie Sercombe ,&nbsp;Kazuyo Igawa ,&nbsp;Kenji Izumi","doi":"10.1016/j.talo.2024.100297","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100297","url":null,"abstract":"<div><p>With the development of various radiation-based cancer therapies, radiobiological evaluation methods instead of traditional clonogenic assays with monolayer single cell culture are required to bridge gaps in clinical data. Heterogeneity within cancer tissues is the reason for bridging the gap between basic and clinical research in cancer radiotherapy. To solve this problem, we investigated an evaluation assay using a three-dimensional (3D) model of cancer tissue. In this study, a 3D model consisting of tumor and stromal layers was used to compare and verify radiobiological effects with conventional two-dimensional (2D) methods. A significant difference in the response to radiation was observed between the 2D and 3D models. The relative number of cancer cells decreased with X-ray dose escalations in the 2D and 3D models. In contrast, the relative number of normal cells was quite different between the 2D and 3D models. Considering the ability of cells to recover from radiation-induced damage, the histological results of the 3D model were reflected in the clinical data. Histopathological analysis using a 3D model is a potential method for evaluating radiobiological effects on the tumor and tumor margins.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100297"},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000110/pdfft?md5=bc5883f91ca2ca484638358c38d000fe&pid=1-s2.0-S2666831924000110-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139898466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic analysis of counterfeit psychotropic drugs","authors":"Klara Dégardin,&nbsp;Aurélie Guillemain,&nbsp;Alexandra Feng,&nbsp;Christian Saladin,&nbsp;Stéphanie Paratte,&nbsp;Raphaël Zurbach,&nbsp;Tobias Mohn","doi":"10.1016/j.talo.2024.100295","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100295","url":null,"abstract":"<div><p>Medicine counterfeiting is a worldwide problem affecting all therapeutic areas. The analysis of such products is sometimes a scientific challenge, since their chemical composition is unknown and might be very different from the one of the original product. While some counterfeited drug products are quite easy to detect, others are close in composition with the genuine products. The health consequences of counterfeit medicines are appalling since these products can contain the =rong chemical composition, impurities or toxic compounds, and be manufactured and stored in dreadful conditions that do not even respect the basic hygienic conditions. Providing reliable analytical tools is therefore necessary for an efficient fight against the phenomenon. In this study, the analysis of counterfeits of psychotropic drugs presenting a rare closeness in composition to the genuine drugs will be described. It will be exposed how the choice of reliable analytical strategy and tools is decisive for the correct authentication of such pharmaceuticals. The results of classical methods like High-Performance Liquid Chromatography (HPLC) will be presented. Then the contribution of vibrational spectroscopy and imaging tools, including spectral imaging and Near infrared (NIR) spectroscopy, and spectral imaging, will be described, as well as Gas Chromatography coupled with Mass Spectrometry (GC–MS), 1H Nuclear Magnetic Resonance (NMR), 3D microscopy as support for the physical analysis, and finally X-ray fluorescence spectroscopy (XRF) as an elemental analysis technology. The results of the forensic analysis will also reveal analytical links between the counterfeit cases, providing useful information for law enforcement investigations.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100295"},"PeriodicalIF":0.0,"publicationDate":"2024-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000092/pdfft?md5=ceca2d12588f4e5082392bc1d02c34be&pid=1-s2.0-S2666831924000092-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139743830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An equation for fitting distance-based measurements with analyte concentrations: From discrete segments simulation to closed-form solution","authors":"Prapin Wilairat","doi":"10.1016/j.talo.2024.100296","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100296","url":null,"abstract":"<div><p>In this study, an equation for fitting the band lengths in µPADs to the concentrations/amount of analyte added to the µPAD sample area is derived. A simulation of the band formation is carried out using a discrete segment model. The detector channel is divided into equal segments with the same amount of reagent R in each segment. The sample moves into the channel in steps corresponding to segments of the same size as the detector segment. Each sample segment contains analyte A at C mole ratio to reagent R. Assuming a stoichiometric ratio of 1:1 for reaction between A and R, there will be formation of only one product band in each detector segment. By examining the number of bands (n) formed after N steps, a set of linear algebraic equations is derived to determine the number of bands (n) for any integer values of N and C. By extrapolating this result to real positive numbers, we obtain the equation <em>L</em>=<em>a</em>.C_A/(<em>b</em> + C_A), where L represents the band length, and C_A represents the concentration/amount of analyte. The equation represents a rectangular hyperbola.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":0.0,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000109/pdfft?md5=00011778d290d62fe9d5fbb6a7dfe315&pid=1-s2.0-S2666831924000109-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139714507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validated spectrophotometric approach for the estimation of an antiepileptic drug: retigabine in pure form and pharmaceutical formulations utilizing N-Bromosuccinimide as an oxidant","authors":"Mohannad M. Garoub ,&nbsp;Ragaa El Sheikh ,&nbsp;Sara G. Mohamed ,&nbsp;Moataz S. Mahmoud ,&nbsp;Ahmed F. Abdel Allem ,&nbsp;Ahmed El Sayed ,&nbsp;Ahmed A. Ghazy ,&nbsp;Nessma M. Gomaa ,&nbsp;Sameh Abdalla ,&nbsp;Osama M.A. Salem ,&nbsp;Ayman A. Gouda","doi":"10.1016/j.talo.2024.100294","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100294","url":null,"abstract":"<div><p>A spectrophotometric method that is sensitive, easy, accurate, exact, reproducible, and verified has been developed for quantifying a new antiepileptic medication called retigabine in both its pure form and pharmaceutical formulations. The techniques employ N-bromosuccinimide (NBS) as an oxidizing agent and three dyes, namely amaranth, methylene blue, and indigo carmine. The three methods rely on the oxidation reaction of retigabine with an excess of N-bromosuccinimide (NBS) in an acidic environment. The unreacted NBS is then quantified by reacting it with fixed amounts of dyes, specifically amaranth, methylene blue, and indigo carmine. The absorbance at 520 nm, 664 nm, and 610 nm is measured for each respective dye. Linear relationships with high correlation coefficients (0.9992-0.9997) were observed under optimal conditions across concentration ranges of 0.5-12, 0.5-16, and 0.5–10 µg/ml. The limit of detection (LOD) for amaranth, methylene blue, and indigo carmine methods were determined to be 0.15, 0.15, and 0.14 µg/ml, respectively. The accuracy and precision of the approaches have been assessed for both intra-day and inter-day measurements. There was no observable interference caused by the typical tablet excipients. The proposed methodologies were verified in compliance with ICH recommendations and effectively utilized for the analysis of retigabine in pharmaceutical formulations. The reliability of the approaches was confirmed by conducting recovery studies employing the usual addition method. The findings produced by the proposed methods were statistically compared to those of the described approach using the student's t-test and F-test, which showed a significant level of agreement.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100294"},"PeriodicalIF":0.0,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000080/pdfft?md5=1f0d4ecf4534f34969eb9ce600700d4b&pid=1-s2.0-S2666831924000080-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139737167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of modified Triethanolamine on grinding efficiency and performance of cementitious materials","authors":"Yahya Kaya ,&nbsp;Veysel Kobya ,&nbsp;Ali Mardani ,&nbsp;Joseph J. Assaad","doi":"10.1016/j.talo.2024.100293","DOIUrl":"10.1016/j.talo.2024.100293","url":null,"abstract":"<div><p>This paper seeks to improve through esterification the efficiency of triethanolamine (TEA) molecules used in the cement grinding industry. The unmodified and modified-TEAs are tested at 0.025 % to 0.1 % rates to determine their effect on clinker grindability and cement properties including fineness, flow, zeta potential, morphology, rheology, setting, and compressive strength. Test results showed that the esterification reactions performed to modify the TEA hydroxyl groups can remarkably increase their adsorption potentials onto the clinker grains, which reduces the agglomeration phenomenon and grinding energy consumption by 6 % to 13 %. The TEA modified using medium hydrocarbon chain lengths (4–8 carbons) of mono-carboxylic acid compounds performed better than the one containing short hydrocarbon chain lengths (1–3 carbons). The pastes and mortars prepared using the modified-TEA cements exhibited significantly higher fluidity, better compatibility with polycarboxylate-based superplasticizer, and increased strengths than the equivalent mixtures made using cement ground using the unmodified TEA molecules.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100293"},"PeriodicalIF":0.0,"publicationDate":"2024-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000079/pdfft?md5=5cee88dfe6af359aebb78c4aec7e43f0&pid=1-s2.0-S2666831924000079-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139677650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bimetallic MOFs-derived four-in-one CeCuOx/C nanozyme and its application for colorimetric detection of cysteine based on oxidase mimics","authors":"Wen-Xuan Jia,&nbsp;Ming-Ming Tao,&nbsp;Xin-Yu Wang,&nbsp;Yan-Feng Huang,&nbsp;Ying Li","doi":"10.1016/j.talo.2024.100292","DOIUrl":"10.1016/j.talo.2024.100292","url":null,"abstract":"<div><p>To address the limitations of natural enzymes, more and more artificial enzymes have been developed. As the most appropriate candidate, metal organic frameworks (MOFs) and MOFs-based derivatives have generated extensive interest due to their remarkable properties and potential applications in enzymes mimicry. In this work, Ce/Cu mixed metal oxides-carbon composite (CeCuO_x/C) was synthesized through a simple oxidation method with bimetallic MOFs CeCu-BTC as precursors. It was found that the prepared CeCuO_x/C possess four different enzyme-like activities, including peroxidase, oxidase, laccase and phosphatase mimetic activities. The kinetics parameters and potential influence factors of enzyme-like activities were studied and CeCuO_x/C exhibited high catalytic activity, excellent stability, and reusability. Additionally, a cysteine detecting method was established via oxidase-like activity of CeCuO_x/C. The linear response to the cysteine was in the range of 50 to 900 μM, and the limit of detection was 3.24 μM. The spiking recoveries were in the range 93.5–100.2 % for cysteine in artificial human urine samples and the relative standard deviations were found within the ranges of 1.2–2.9 %. This work presents a new approach for the design of multi-enzyme mimics and develops the potential of CeCuO_x/C for biological sensing.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100292"},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000067/pdfft?md5=74f5ea83013f4f48201c9a2e0c3341e6&pid=1-s2.0-S2666831924000067-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139658194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroanalysis overview: The determination of the poisoner's poison, thallium","authors":"Robert D. Crapnell,&nbsp;Craig E. Banks","doi":"10.1016/j.talo.2024.100291","DOIUrl":"10.1016/j.talo.2024.100291","url":null,"abstract":"<div><p>In this overview, we explore the electroanalytical determination of the poisoner's poison: thallium. Thallium was named after the Greek word \"thallos,\" meaning \"green shoot\" or \"twig,\" due to its bright green spectral emission lines. It is toxic, tasteless, odourless and dissolves into water, and has been used by murderers as a challenging poison to detect and there is the need for the analytical determination of thallium. Laboratory based analytical instrumentation provide a routine methodology to measure thallium, but there is scope to develop in-the-field analytical measurements that are comparable to laboratory equipment and in some cases, they can provide even more sensitive analytical approaches. Electrochemistry can support such endeavours, where instrumentation are readily portable where electroanalytical sensors provide highly selective and sensitive outputs but yet are economical to support on-site analysis. In this review, we provide an electroanalytical overview of the current research directed toward the measurement of thallium and offer insights to future research.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100291"},"PeriodicalIF":0.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000055/pdfft?md5=23507a9dfded8cfa9fcb62f63dcbdb73&pid=1-s2.0-S2666831924000055-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139647956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal oxide/g-C3N4 nanocomposites chemiresistive gas sensors: A review on enhanced performance","authors":"Vahid Khoramshahi ,&nbsp;Majid Azarang ,&nbsp;Morteza Nouri ,&nbsp;Abbas Shirmardi ,&nbsp;Ramin Yousefi","doi":"10.1016/j.talo.2024.100290","DOIUrl":"10.1016/j.talo.2024.100290","url":null,"abstract":"<div><p>Metal-oxide-semiconductors (MOS) gas sensors are widely used for detecting and measuring the concentration of various gases in different applications. Changing the electrical resistance when the MOS surface reacts with a specific gas is the basis of the operation of the gas sensor of MOS. They offer versatility in detecting various gases and fabricating them suitable for supervising energy efficiency, monitoring health and safety, and controlling hazardous emissions. However, traditional MOS sensors suffer from poor selectivity and usually require high operating temperatures. To overcome these limitations, researchers have explored strategies such as doping, bimetallic/co-doping, and composite structures with conductive polymers and 2D materials such as polyaniline (PANI), polymethyl methacrylate (PMMA), polyvinyl alcohol (PVA), reduced graphene oxide (rGO), graphitic carbon nitride (g-C₃N₄), and graphene. Among the 2D materials, g-C₃N₄ stands out due to its distinct characteristics, including chemical stability, porosity structure, abundance, lack of toxicity, and numerous surface defects. The exfoliated structure and surface defects of g-C₃N₄ provide active sites for adsorbing atmospheric oxygen and facilitating reactions with specific gas molecules. This review introduces MOS gas sensors, covering their fabrication methods and electrical measurements. It then attentions on the properties of g-C₃N₄, synthesis methods, and its potential for composition with the MOS. The review highlights the enhanced gas sensing performance achieved by MOS/g-C₃N₄ nanocomposites to detect different gases.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100290"},"PeriodicalIF":0.0,"publicationDate":"2024-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000043/pdfft?md5=303061131a225801e102618695ae81a2&pid=1-s2.0-S2666831924000043-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139580926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut-liver interaction study on an all-polydimethylsiloxane microfluidic device integrating intestinal paracellular permeability assay","authors":"Ryuya Kida ,&nbsp;Alan Rajendran ,&nbsp;Mamiko Tsugane ,&nbsp;Jean-Charles Duclos-Vallée ,&nbsp;Maxime M Mahe ,&nbsp;Sakina Bensalem ,&nbsp;Hiroaki Suzuki ,&nbsp;Bruno Le Pioufle","doi":"10.1016/j.talo.2024.100289","DOIUrl":"10.1016/j.talo.2024.100289","url":null,"abstract":"<div><p>Microphysiological systems (MPSs) have attracted increasing attention as a method for simulating in vitro drug efficiency. In particular, the interaction between liver and intestine tissues is one of the primary targets since they are closely involved in drug absorption and metabolism. However, most of the intestine-liver MPSs reported previously require pumps, electrodes, and porous membranes for co-culture of cells and evaluation of intestinal permeability (i.e., Trans-Epithelial Electrical Resistance, TEER), requiring complex manufacturing processes and operations. In this study, we report an all-polydimethylsiloxane (PDMS) co-culture microfluidic device, connecting microchamber-based paracellular transport assay on gut microtissues to liver tissues matured on the same device. On one side of the device, HepaRG cells are confined within thin parallel grooves that induce their differentiation into hepatocytes. The other side of the device is connected with microchannels to the liver side and includes the gut tissues, organized above microchambers. Such microchambers allow the evaluation of paracellular permeability by fluorescence imaging. Thanks to the microfluidic device we investigated changes in intestinal permeability induced by differentiated hepatocyte excretion and found that Caco-2 permeability was decreased when co-culture with HepaRG. Due to its simplicity and straightforward implementation, this method is anticipated as an innovative and efficient approach to assess tissue barrier function in multi-organ on-chip experiments.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100289"},"PeriodicalIF":0.0,"publicationDate":"2024-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000031/pdfft?md5=bea30a8981f794692ca2ed895fb0cee4&pid=1-s2.0-S2666831924000031-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139539343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NNNPERFUME: Detection of 8-oxoguanine DNA glycosylase activity based on primer exchange reaction and functionalized hemin/G-quadruplex DNAzyme","authors":"Kaiting Xiao ,&nbsp;Yanming Lai ,&nbsp;Xingxing Liu,&nbsp;Shengjie Li,&nbsp;Wenxu Yuan,&nbsp;Ziyun Wang,&nbsp;Pan Pan,&nbsp;Yongkui Li,&nbsp;Heng Xiao","doi":"10.1016/j.talo.2024.100288","DOIUrl":"10.1016/j.talo.2024.100288","url":null,"abstract":"<div><h3>Background</h3><p>8-oxoguanine DNA glycosylase can maintain genomic stability and integrity. However, it can interfere with the regular DNA damage repair process, possibly leading to the development of cancer and various other human diseases when its activity becomes abnormal. Current methods for detecting 8-oxoguanine DNA glycosylase activity often suffer from low sensitivity, time-consuming procedures, labor-intensive requirements, and the need for specialized equipment and trained professionals for execution. Consequently, there is an urgent need for a portable, user-friendly 8-oxoguanine DNA glycosylase assay that offers swift results and supports real-time testing.</p></div><div><h3>Results</h3><p>We've developed a PERFUME method that combines <strong>p</strong>rimer <strong>e</strong>xchange <strong>r</strong>eaction and <strong>f</strong>unctionalized G-q<strong>u</strong>adruplex/he<strong>m</strong>in DNAzym<strong>e</strong> for sensitive detection of Fpg, a typical 8-oxoguanine DNA glycosylase. Utilizing a single probe and T4 Polynucleotide Kinase (PNK) simplifies the experiment to a one-step reaction at 37 °C in 3 h, reducing sample consumption and improving sensitivity. We chose functionalized hemin cofactors, significantly improving catalytic efficiency and enhancing detection capability. This biosensor detects Fpg activity with a sensitivity as low as 0.11 U mL⁻¹, displaying exceptional sensitivity, selectivity, and interference resistance in human serum and bacterial cell extracts under isothermal conditions. The biosensor demonstrates remarkable selectivity and ability for Fpg inhibitors screening. In addition, this biosensor enables reading the sample's RGB values using a smartphone, facilitating accurate quantification of Fpg activity without the necessity for specialized equipment.</p></div><div><h3>Significance</h3><p>PERFUME simplifies Fpg detection by using a single hairpin and PNK in a one-step process. We utilize FUME to enhance catalytic efficiency, it surpassing the performance of traditional G-quadruplex/hemin DNAzyme methods. This approach excels in analyzing Fpg in human serum and bacterial extracts. It allows quantitative Fpg detection using UV–Vis and smartphones under isothermal conditions, making it valuable for clinical diagnosis.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100288"},"PeriodicalIF":0.0,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266683192400002X/pdfft?md5=2888c2a68f62e637d2c8f099a2588def&pid=1-s2.0-S266683192400002X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139471093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}