Talanta Open最新文献

{"title":"Upconverting nanophosphors for various sensing applications","authors":"Jitender Kumar,&nbsp;Indrajit Roy","doi":"10.1016/j.talo.2024.100302","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100302","url":null,"abstract":"<div><h3>Background</h3><p>Upconverting nanophosphors (UCNPs) are composed of certain nanosized inorganic host materials doped with specific rare earth ions. They exhibit the phenomenon of photon upconversion, whereby the sequential absorption of two or more low energy photons is followed by luminescent emission of multiple photons with higher energy. In addition, they have other attractive optical features such as NIR-absorption, sharp emission bands, exceptional photostability and high quantum yield. These properties make UCNPs lucrative probes for number of optical applications, such as imaging, sensing and theranostics.</p></div><div><h3>Overview</h3><p>UCNPs have been used as specific and ultrasensitive probes for sensing of variety of analytes, such as gas molecules, metal ions, fine particles, pH and a number of molecules, biochemicals and macromolecules. Their absorption and emission in the NIR range facilitates the background free biosensing in situ, in vitro and in vivo. Their multicoloured emissions allow a suitable emission band to serve as a donor for energy transfer to another attached optical probe, while a separate emission band to serve as an internal reference in ratiometric sensing applications. This comprehensive review provides an in-depth exploration of the versatile applications of UCNPs in the optical sensing of various analytes based on energy transfer mechanisms, with emphasis on the recent developments. . The focus is on synthesizing UCNPs, surface functionalization, and their effective utilization especially in detecting gas molecules, pH changes, ions, free radicals, and disease biomarkers. Through a meticulous examination of the use of UCNPs, this review aims to contribute to a deeper understanding of their potential and pave the way for future advancements and challenges in optical sensing.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100302"},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266683192400016X/pdfft?md5=6fdb848bbbefc5b9c0fe0228707580fe&pid=1-s2.0-S266683192400016X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140067162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developing a prediction method for physicochemical characteristics of Pontianak Siam orange (Citrus suhuiensis cv. Pontianak) based on combined reflectance-Fluorescence spectroscopy and artificial neural network","authors":"Sandra ,&nbsp;Abdullah Said ,&nbsp;Ahmad Avatar Tulsi ,&nbsp;Dina Wahyu Indriani ,&nbsp;Rini Yulianingsih ,&nbsp;La Choviya Hawa ,&nbsp;Naoshi Kondo ,&nbsp;Dimas Firmanda Al Riza","doi":"10.1016/j.talo.2024.100303","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100303","url":null,"abstract":"<div><p>The slightly sweet and acidic taste offered by Pontianak Siam oranges is influenced by the total soluble solids (TSS) and acidity in the fruit, in which, measuring these attributes is commonly performed using instruments that potentially damage the fruit's structure, thus, impractical for fresh fruit products. Moreover, the process of classifying the quality of fresh oranges has been based on physical appearance, leading to subjective results. Correspondingly, the objective of the study is to develop a prediction method for the physicochemical characteristics of Pontianak Siam oranges based on VIS-NIR-Fluorescence spectroscopy and an artificial neural network (ANN) model. The method is applicable to classify oranges based on physicochemical characteristics without damaging the fruit's structure. As a result, the best model for classifying the maturity level of Pontianak Siam oranges was obtained using a dataset with <em>all feature</em> combined spectra, attaining a training accuracy of 0.99 and testing accuracy of 1. The best model for predicting TSS was obtained using <em>all feature</em> combined spectra dataset, attaining R² training = 0.89 and R² testing = 0.91. The best model for predicting acidity was obtained using <em>all feature</em> reflectance spectra datasets, attaining R² <em>training</em> = 0.96 and R² <em>testing</em> = 0.97. The best model for predicting fruit firmness was obtained using <em>all feature</em> reflectance spectra dataset, attaining R² <em>training</em> = 0.97, R² <em>testing</em> = 0.89. Overall, the combination of Vis-NIR reflectance and fluorescence spectroscopy have the potential to be applied for non-destructive assessment of citrus quality in terms of visual classification and maturity parameters prediction.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100303"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000171/pdfft?md5=9ec70aeb96eee693412d16eba5fcef40&pid=1-s2.0-S2666831924000171-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Future advances of artificial biosensor technology in biomedical applications","authors":"Smriti Gaba ,&nbsp;Nidhi Chauhan ,&nbsp;Ramesh Chandra ,&nbsp;Utkarsh Jain","doi":"10.1016/j.talo.2024.100301","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100301","url":null,"abstract":"<div><p>Recent advancements in synthetic biology have facilitated the concept of a cell-based and cell-free biosensing platform, which enables the identification of molecular signals encompassing metal/chemical to disease biomarkers. The artificial sensing incorporates the concept of both whole-cell and cell-free biosensing strategies, which include highly regulated natural and synthetic components to exhibit genetically encoded molecular sensing properties. These sensors utilize protein expression to release signalling molecules as the result of received input to facilitate the detection of analytes. Intending to use modified living cells or artificial cells in biosensing, the proposed study highlights the importance of cell-based and cell-free sensors in biomedical and diagnostics. The article's first section will explain the biosensing types including cell-free, cell-based, vesicle-based, and paper-based sensing where sensing relies on cell, cellular components, and cell-free systems which mostly involve transcriptional or translational machinery. It highlights the advantages, disadvantages, and challenges of advancing approaches. The second section of the article elaborates on the principle of sensing and the strategies involved. Though very few studies have been reported on this topic, therefore, the current article focuses on the artificial sensors that have been designed for medical and diagnostic purposes. The review also marks the current and future advancements in the field including artificial intelligence, nanotechnology, stem cells, and omics. Sensing recently has a big impact on disease diagnosis as well as drug development and targeted therapies. While newly developed biology-based diagnostics technologies still have high costs, require highly trained personnel, suffer stability issues and reduce sensor performance. Therefore, this review brings readers’ attention to advances and challenges in the following field and promotes the resolution of medical and diagnostics issues in the future.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100301"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000158/pdfft?md5=ed4485009f4ecc8daaa5ea9b455b26a4&pid=1-s2.0-S2666831924000158-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140062396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fine-tuning of highly bright benzo[c,d]indole-oxazolopyridine cyanine dye for nucleolar RNA imaging in living cells","authors":"Nao Togashi,&nbsp;Masaaki Nagaoka,&nbsp;Kei Higuchi,&nbsp;Yukina Yoshino,&nbsp;Yawen Wu,&nbsp;Yusuke Sato,&nbsp;Seiichi Nishizawa","doi":"10.1016/j.talo.2024.100308","DOIUrl":"10.1016/j.talo.2024.100308","url":null,"abstract":"<div><p>Here we report on fine-tuning of highly bright fluorogenic cyanine dye, benzo[<em>c,d</em>]indole-oxazolo[5,4-<em>c</em>]pyridine (BIOP: <em>λ</em>_em = 570 nm, Φ_free = 0.00038, Φ_bound = 0.52), recently developed by our group for nucleolar RNA imaging in living cells. We tuned an emission maximum to the longer wavelength by replacing oxazolopyridine unit with its isomer. The resulting probe with oxazolo[4,5-<em>b</em>]pyridine, named BIOP [4,5-<em>b</em>], exhibited a significant off-on signaling ability for RNA (<em>λ</em>_em = 580 nm, Φ_free = 0.002, Φ_bound = 0.46) that almost compared with BIOP. BIOP [4,5-<em>b</em>] was applicable to live-cell imaging, where wash-free protocol was available. Importantly, the slight change in spectral features would be expected to minimize the false signal from BIOP [4,5-<em>b</em>] in co-staining experiments with typical green-emissive dyes. In addition, thanks to the unique spectral shape that is typical of cyanine dyes, we demonstrate that yellow-emissive BIOP [4,5-<em>b</em>] also did work as a pseudo red-emissive dye (<em>λ</em>_em &gt; 600 nm) for live-cell RNA imaging, for which we just switch filter set to typical one for real red-emissive dyes (Ex 560/40 nm; Em 645/75 nm).</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100308"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000225/pdfft?md5=b81ca6c609cbce71b0eaf03b46b885ac&pid=1-s2.0-S2666831924000225-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140010472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-step dual-layer SPE method to separate antibacterial and antioxidant mushroom compounds","authors":"Dániel Krüzselyi,&nbsp;Péter G. Ott,&nbsp;Ágnes M. Móricz","doi":"10.1016/j.talo.2024.100304","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100304","url":null,"abstract":"<div><p>We developed a two-step dual-layer solid-phase extraction (SPE) method to separate and concentrate minor bioactive compounds of golden oyster mushroom (<em>Pleurotus citrinopileatus</em>) methanol extract. The mushroom extract is rich in fatty acids, including the highly abundant (20–35 %) and antibacterial linoleic acid. To eliminate the masking effect of linoleic acid in the bioassay, its removal was achieved by homemade dual-layer SPE that consisted of a lower C₁₈ phase and an upper silica gel phase with the dried-on extract. In the first step the elution was performed with water (aqueous eluate), and then the dried C₁₈ and silica gel phases were eluted separately with methanol (C₁₈ methanol eluate and silica gel methanol eluate, respectively). Thin-layer chromatography (TLC)-effect-directed analysis demonstrated that the aqueous eluate contained sugars and antioxidants (TLC-DPPH); fatty acids were present in the silica gel methanol eluate, in the C₁₈ methanol eluate the biologically active minor secondary metabolites were concentrated (TLC–<em>Bacillus subtilis</em> assay) beside 2 % remained linoleic acid according to HPLC-UV measurement. As a result, the antioxidant and antibacterial metabolites divided, and the low-abundant antibacterial compounds could be detected. Based on this study, the substance found at <em>R</em>_F 0.25 by TLC-DPPH played the most significant role in generating the antioxidant effect of the aqueous eluate, equivalent to 5.07 ± 0.09 µg of gallic acid. Moreover, each SPE fraction and raw extract were tested for antibacterial effect using a microplate assay with a non-pathogenic Gram-positive <em>Bacillus subtilis</em>. The <em>P. citrinopileatus</em> extract (MIC-value – 50 µg/mL) and C₁₈ methanol eluate (MIC-value - 25 µg/mL) inhibited the growth of <em>B. subtilis</em> while the other eluates did not.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100304"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000183/pdfft?md5=e1e539d1d5dc0fd0d0766e4532f5c14d&pid=1-s2.0-S2666831924000183-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140014326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"User-friendly diameter-based measurement paper sensor for chloride detection in water","authors":"Benjarat Tasangtong ,&nbsp;Thanapit Pholsaptanakorn ,&nbsp;Thanakorn Tapsawut ,&nbsp;Nisakorn Wiwekwin ,&nbsp;Jaruwan Mettakoonpitak ,&nbsp;Piyaporn Na Nongkhai ,&nbsp;Yupaporn Sameenoi","doi":"10.1016/j.talo.2024.100305","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100305","url":null,"abstract":"<div><p>Chloride (Cl⁻) is an anion widely distributed in nature. It is also an essential parameter to consider when assessing the water quality for ensuring drinking water safety, preventing infrastructure damage, mitigating environmental impact, identifying groundwater contamination. This work presents the first development of a diameter-based measurement paper sensor for chloride analysis using the reaction based on a Mohr's precipitation titration. The paper sensor that has a circular shape with 3-cm diameter was pre-coated with AgNO₃ and CrO₄²⁻ forming brown precipitates of the Ag₂CrO₄. The sensor was sealed using lamination films with 3-mm diameter hole-punched inlets on the top of the lamination film for sample delivery. To detect chloride, the sensor was simply immersed into the sample. The chloride solution flows into the central sample inlet and spreads radially to undergo the displacement reaction with Ag₂CrO₄ precipitate, forming AgCl white precipitate whose diameter proportional to the chloride that can be observed within 3 min. Concentration of AgNO₃ used was found to impact the analytical figures of merit. The lower AgNO₃ concentration yields lower limit of detection, narrower linear range but higher sensitivity. The sensor was applied for chloride analysis in tap water, drinking water and industrial water and the chloride concentration obtained from the developed sensors are not significant differences from those obtained from the standard titration method at 95% confidence interval (two tailed <em>P</em> = 0.08) indicating that the developed sensor provides accurate analysis of chloride in water samples from various sources. The developed sensor was used by the untrained staffs for on-site of analysis chloride in tap water collected at 26 locations in SaenSuk Municipality area, Chonburi, Thailand. The results showed that the chloride level in all samples is in range of 52.2–84.7 mg L⁻¹ which is far below the acceptable range set by the Provincial Waterwork Authority of Thailand (&lt; 250 mg L⁻¹) indicating that the tap water used in this area is safe for consumers.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100305"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000195/pdfft?md5=abc4f1c488ceb7dfe2227102f243217d&pid=1-s2.0-S2666831924000195-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140062353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed cartridges for the removal of pharmaceuticals from water","authors":"Ankur Jyoti Thakuria ,&nbsp;Purushottam Suryavanshi ,&nbsp;Subham Banerjee","doi":"10.1016/j.talo.2024.100299","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100299","url":null,"abstract":"<div><p>The entry of pharmaceuticals into water is a key worldwide concern, with drugs being identified in all near-aqueous ecologies at often worrying concentrations. Pharmaceutical waste in environmental water has been shown to affect environmental equilibrium and pose a risk to humans adversely. In the present study, we fabricated a novel system containing 3D printed sustainable, porous Polyethylene terephthalate glycol (PETG) cartridges and RP-HPLC-DAD methodology to remove non-steroidal anti-inflammatory drugs (NSAIDs) such as diclofenac sodium (DS) and indomethacin (IND) from water samples called “Cartridges.” The device was fabricated using fused deposition modeling (FDM)-mediated 3D printing technology using indigenous hot-melt extruded filaments. To enable FDM 3D printing, the filaments were fabricated using PETG as a primary material and polyvinyl alcohol (PVA), Affnisol™, and Eudragit®EPO as water-soluble pore-forming materials. We showed that the thermoplastic, recyclable PETG composite material, which presents microporous properties after removal of PVA, Affnisol™, and Eudragit®EPO, is effective for extracting both drugs from water solution. The usefulness of the current strategy was demonstrated by the extraction of DS and IND from an aqueous solution followed by RP-HPLC analysis. The% extraction recovery was more than 80 % for both drugs, with an overall relative standard deviation of less than 3 %. The proposed approach can bring a ray of hope in extraction and sample preparation in the analytical industry due to a shift to the use of sustainable cartridges with customizable shapes, sizes, and chemical properties.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000134/pdfft?md5=c0f1753250c9a59a9137682db6c61aed&pid=1-s2.0-S2666831924000134-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140031578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Calibration of a passive sampling device for the determination of nitrogen dioxide in ambient air","authors":"Thanh-Binh Huynh ,&nbsp;Bao-Thuyen Vo-Ngoc ,&nbsp;Trung Dang-Bao ,&nbsp;Thi-Kieu-Anh Tran","doi":"10.1016/j.talo.2024.100306","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100306","url":null,"abstract":"<div><p>Nitrogen dioxide (NO₂), a common air pollutant, has been widely admitted to be harmful to both the environment and human health, demanding its well-control procedure and corresponding quantification. In this study, NO₂ in ambient air was collected by a passive sampling method using the Willems badge diffusive sampler, followed by a derivatization step with the Griess-Saltzman solution, and analyzed by ultraviolet-visible (UV–vis) spectroscopy at 543 nm. The device can be utilized for 168 h of continuous field sampling. The experimental sampling rate (K_e) of (4.02 ± 0.29) × 10⁻³ m³ <em>h</em> ⁻ ¹ with a relative standard deviation (% RSD) of 9.6 % was determined by conducting parallel experiments between an active sampling method (ISO 6768:1998) and the Willems samplers. After exposure time, samplers could be stored for two weeks in a refrigerator at 4 °C before analyzing. The studied passive diffusive sampler was simple, low-cost, easy to reuse; permitted determining the average concentration of NO₂ in ambient air. The average NO₂ concentrations for 2-hour to 4-hour sampling periods at different studied sampling sites in Ho Chi Minh city (Vietnam) were ranged from 11.5 to 189 μg <em>m</em> ⁻ ³.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100306"},"PeriodicalIF":0.0,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000201/pdfft?md5=a428752380ab3c2d1f570498a872ac3c&pid=1-s2.0-S2666831924000201-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139992965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fixed combination of rivaroxaban and Aspirin: Tablet formulation and analytical method validation","authors":"Hani Naseef ,&nbsp;Mosab Afaneh ,&nbsp;Moammal Qurt ,&nbsp;Abdullah Rabba ,&nbsp;Abdallah Abukhalil ,&nbsp;Numan Malkieh ,&nbsp;Maher Kharouf ,&nbsp;Ramzi Moqadi","doi":"10.1016/j.talo.2024.100298","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100298","url":null,"abstract":"<div><p>Rivaroxaban and aspirin are commonly used antithrombotic agents in combination to prevent coronary artery disease (CAD) and atherothrombotic events in adult patients after acute coronary syndrome (ACS) with elevated cardiac biomarkers, or with coronary artery disease (CAD) or symptomatic peripheral artery disease (PAD) in high-risk patients. The recommended dosage is 2.5 mg rivaroxaban twice daily in combination with 75–100 mg aspirin once daily. This study aimed to develop a fixed-dose combination tablet of rivaroxaban (2.5 mg) and aspirin (50 mg) to enhance medication adherence and decrease pill burden.</p><p>Compatibility studies were conducted on the active ingredients to develop the product formula. The formula and the manufacturing procedure were chosen based on the risk assessment for each active substance, wet granulation with both actives intragranular was found to have faster dissolution than direct mix formulae. Furthermore, a validated reverse-phase HPLC stability indicator method was developed to detect APIs and their possible degradants.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100298"},"PeriodicalIF":0.0,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000122/pdfft?md5=8f7c6548a68dc9ec5e8462a066724532&pid=1-s2.0-S2666831924000122-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139975936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Method development for an untargeted HS-SPME-GC–MS analysis of terpenes and cannabinoids for the geographical sourcing of Marijuana","authors":"Janet Crespo Cajigas,&nbsp;Vidia A. Gokool ,&nbsp;Howard K. Holness,&nbsp;Kenneth G. Furton,&nbsp;Lauryn E. DeGreeff","doi":"10.1016/j.talo.2024.100300","DOIUrl":"https://doi.org/10.1016/j.talo.2024.100300","url":null,"abstract":"<div><p>Despite growing decriminalization of <em>Cannabis sativa</em> (i.e., marijuana) possession throughout the United States of America, there remains to be an ongoing interest in the detection of unlawfully possessed and transported marijuana. This issue has resulted in an increasing interest regarding the generalization and specification related to the canine detection of marijuana. More specifically, canine trainers have expressed concerns on whether canines can generalize on the odor of marijuana regardless of the origin of their training materials. This research aims to differentiate multiple marijuana headspace samples from three regions in the USA based solely on the volatile organic compounds (VOCs) found in their odor profiles. In this study, a heated headspace solid phase micro-extraction (SPME) technique was optimized and implemented for the collection of both volatile terpenes and cannabinoids from marijuana. The headspace samples were analyzed using two full-scan, untargeted, optimized methods on a gas chromatograph coupled to a mass spectrometer (GC–MS), and a variety of chemometric tools were applied to the data to enable differentiation and potential classification between sample populations. Principal component analysis and sparse partial least squares discriminant analysis (sPLS-DA) employed in this study have demonstrated a disparity between marijuana varieties based on geography using the VOCs extracted from their odor profiles. With this research, it is intended to determine some fundamental differences between <em>Cannabis</em> of different geographical origins and set a foundation for the development and advancement of instrumental applications for other non-contact marijuana detection techniques in support of the improvement of illicit substance detection technology.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"9 ","pages":"Article 100300"},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000146/pdfft?md5=4caeba402d699a0d0883a49abe1554c0&pid=1-s2.0-S2666831924000146-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139975935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}