Talanta Open最新文献

Sensitive detection Cu/Zn SOD mRNA with CRISPR/Cas9 利用CRISPR/Cas9灵敏检测Cu/Zn SOD mRNA

IF 3.7

Talanta Open Pub Date : 2025-09-29 DOI: 10.1016/j.talo.2025.100568

Geng Qiuyu , Xing Hanxin , Cang Song , Fan Ronghua , Zheng Anqi

引用次数: 0

The versatility of metal-organic frameworks-based biosensor for antioxidant detection 基于金属-有机框架的抗氧化剂检测生物传感器的多功能性

IF 3.7

Talanta Open Pub Date : 2025-09-26 DOI: 10.1016/j.talo.2025.100566

Siti Unvaresi Misonia Beladona , Rizki Rachmad Saputra , Aep Patah , Meiyanti Ratna Kumalasari

{"title":"The versatility of metal-organic frameworks-based biosensor for antioxidant detection","authors":"Siti Unvaresi Misonia Beladona , Rizki Rachmad Saputra , Aep Patah , Meiyanti Ratna Kumalasari","doi":"10.1016/j.talo.2025.100566","DOIUrl":"10.1016/j.talo.2025.100566","url":null,"abstract":"<div><div>Antioxidants are crucial in mitigating oxidative stress caused by reactive oxygen and nitrogen species (ROS/RNS), contributing to chronic diseases and biomolecular damage. Beyond their biological significance, antioxidants are widely used in food, cosmetics, and pharmaceuticals to enhance stability and shelf life. Conventional detection methods—such as spectrophotometric assays (DPPH, ABTS, ORAC) and chromatographic techniques (HPLC, LC-MS)—are accurate but suffer from high costs, complex workflows, and limited portability. Biosensors offer a promising alternative, combining high sensitivity, rapid analysis, and real-time monitoring. Among these, metal-organic framework (MOFs) based biosensors stand out due to their tunable porosity, high surface area, and multifunctional design, enabling precise antioxidant detection via fluorescent, colorimetric, electrochemical, or hybrid mechanisms. These sensors achieve enhanced selectivity and signal amplification by integrating MOFs with various functional materials (e.g., graphene, gold nanoparticles (AuNPs)) or biomimetic catalysts (nanozymes), even in complex matrices like biological fluids or food extracts. However, challenges remain in improving commercial applications’ selectivity, stability, and scalability. This review examines MOF-based biosensing platforms, their design strategies, and detection mechanisms while addressing key obstacles in transitioning from lab-scale to real-world deployment. By highlighting recent advances and unmet needs, we aim to guide the development of next-generation biosensors for antioxidant monitoring in clinical, industrial, and environmental settings.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100566"},"PeriodicalIF":3.7,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A straightforward HPLC approach to testing butylated hydroxytoluene, an antioxidant, in pure and topical anti-burn gels; Evaluation of greenness, blueness, and whiteness grades 一种直接的高效液相色谱方法测试纯和局部抗烧伤凝胶中的抗氧化剂丁基羟基甲苯；评价绿色、蓝色和白色等级

IF 3.7

Talanta Open Pub Date : 2025-09-23 DOI: 10.1016/j.talo.2025.100565

Jin-Kui Ma , Xiao-Yang Chen , Nan Zhang , Ahmed Sobhy Darwish , Ayman A. Gouda , Ragaa El Sheikh , Xiao-Chen Huang

{"title":"A straightforward HPLC approach to testing butylated hydroxytoluene, an antioxidant, in pure and topical anti-burn gels; Evaluation of greenness, blueness, and whiteness grades","authors":"Jin-Kui Ma , Xiao-Yang Chen , Nan Zhang , Ahmed Sobhy Darwish , Ayman A. Gouda , Ragaa El Sheikh , Xiao-Chen Huang","doi":"10.1016/j.talo.2025.100565","DOIUrl":"10.1016/j.talo.2025.100565","url":null,"abstract":"<div><div>In lieu of being used in anti-burn gels for medicinal reasons, butylated hydroxytoluene is used as a stabilizer and antioxidant to prolong the gel's lifetime and safeguard its effectiveness by halting the oxidation and degradation of other ingredients, especially fats and oils. It shields the product's texture, color, and smell against alterations brought on by light and air exposure. Antioxidants can be used to guard the product quality during the manufacturing process only and may not need to be controlled during its shelf life. A comprehensive, rapid, clear, and environmentally friendly stability-indicating liquid chromatography (LC) technique has been developed and validated, enabling the quantification of butylated hydroxytoluene levels in topical gels. The pharmaceutical compound underwent a series of evaluations in line with ICH standards. Through isocratic separation, butylated hydroxytoluene and its associated degradants were successfully distinguished. The antioxidant was quantified and validated utilizing the Kromasil C-18 stationary phase at a temperature of 45° C. The isocratic mobile phase functions at a flow rate of 2.0 mL/min. and comprises fixed proportions of filtered water, acetonitrile, and methanol. The overall AES grade of 77, AGREE grade of 0.6, AGREEprep grade of 0.6, CACI grade of 65, AGSA grade of 63.89, MoGAPI grade of 70, BAGI grade of 77.5, and ultimate whiteness of 96.3 collectively underscore the environmental benefits of the LC method. Research suggests that the proposed approach is distinctive, accurate, reliable, robust, and ecologically advantageous, rendering it appropriate for routine quality control assessments.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100565"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145216454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging 同时检测H2S和黏度的比例近红外荧光探针：在环境监测和生物成像中的应用

IF 3.7

Talanta Open Pub Date : 2025-09-23 DOI: 10.1016/j.talo.2025.100564

Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren

{"title":"A ratiometric NIR fluorescent probe for simultaneous detection of H2S and viscosity: Applications in environmental monitoring and bioimaging","authors":"Sha Li, Qin Li, Zhihui Yang, Wei Wen, Miao Yan, Haixian Ren","doi":"10.1016/j.talo.2025.100564","DOIUrl":"10.1016/j.talo.2025.100564","url":null,"abstract":"<div><div>Hydrogen sulfide (H<sub>2</sub>S), a critical gasotransmitter governing physiological functions, presents significant environmental and food safety hazards at aberrant concentrations. Concurrently, mitochondrial viscosity serves as a vital biomarker for cellular pathologies. To address the unmet need for precise dual-parameter monitoring, we engineered a mitochondria-targeted near-infrared (NIR) ratiometric fluorescent probe, <strong>T-N</strong>, enabling simultaneous and discriminative detection of H<sub>2</sub>S and viscosity through discrete emission channels. The probe exhibits ultrarapid response (<30 s), exceptional sensitivity, and superb selectivity against competing analytes. Leveraging H<sub>2</sub>S-triggered chromogenic shifts, <strong>T-N</strong>-immobilized test strips facilitate instrument-free, semi-quantitative assessment of food spoilage, validated across environmental waters and industrially relevant beer samples. Moreover, <strong>T-N</strong> achieves synchronous dual-channel imaging of endogenous/exogenous H<sub>2</sub>S and viscosity dynamics in live cells, with verified mitochondrial localization. This work establishes a versatile sensing platform for tracking H<sub>2</sub>S and viscosity in interconnected environmental, food safety, and biomedical contexts.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100564"},"PeriodicalIF":3.7,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical sensors for the determination of polyphenols as antioxidants from natural sources: A comprehensive review of sensor development and characterization 电化学传感器用于测定天然来源的抗氧化剂多酚：传感器发展和表征的综合综述

IF 3.7

Talanta Open Pub Date : 2025-09-22 DOI: 10.1016/j.talo.2025.100562

Soner Çakar , Mahmut Özacar

{"title":"Electrochemical sensors for the determination of polyphenols as antioxidants from natural sources: A comprehensive review of sensor development and characterization","authors":"Soner Çakar , Mahmut Özacar","doi":"10.1016/j.talo.2025.100562","DOIUrl":"10.1016/j.talo.2025.100562","url":null,"abstract":"<div><div>The rapid, sensitive, and selective quantification of polyphenols has become increasingly important in areas such as food quality control, nutraceutical development, and biomedical diagnostics, due to their exhibiting significant antioxidant properties through their redox-active hydroxyl groups, contributing to a variety of pharmacological effects, including anti-inflammatory, cardioprotective, and chemopreventive activities. This review critically examines recent developments in electrochemical sensor platforms tailored for polyphenol detection in complex biological and environmental samples. Emphasis is placed on nanostructured electrode modifications employing carbon-based nanomaterials (e.g., graphene, carbon nanotubes), metal nanoparticles (Au, Ag, Pt), and metal oxide nanostructures (e.g., TiO₂, ZnO) that facilitate enhanced electron transfer rates, augmented electroactive surface area, and improved sensor stability and reproducibility. The review further explores diverse electrochemical transduction techniques, including cyclic voltammetry, square wave voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy, with comparisons of enzymatic and non-enzymatic sensing approaches. Detailed mechanistic insights into polyphenol electrooxidation pathways, adsorption phenomena, and sensor–analyte interfacial interactions are discussed. Complementary spectroscopic and microscopic characterization methods are highlighted for their roles in elucidating structural, electronic, and surface properties critical to sensor functionality and analytical performance.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100562"},"PeriodicalIF":3.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid forensic characterization of automotive clear coats by ATR-FTIR combined with chemometrics ATR-FTIR结合化学计量学对汽车透明涂层的快速法医鉴定

IF 3.7

Talanta Open Pub Date : 2025-09-22 DOI: 10.1016/j.talo.2025.100563

Polina M. Ilicheva , Kristina T. Musina , Sergey P. Kurchatkin , Natalia A. Burmistrova , Yulia B. Monakhova

{"title":"Rapid forensic characterization of automotive clear coats by ATR-FTIR combined with chemometrics","authors":"Polina M. Ilicheva , Kristina T. Musina , Sergey P. Kurchatkin , Natalia A. Burmistrova , Yulia B. Monakhova","doi":"10.1016/j.talo.2025.100563","DOIUrl":"10.1016/j.talo.2025.100563","url":null,"abstract":"<div><div>Hit-and-run criminal behavior is a challenge for law enforcement agencies around the world. Paint chips left at a crime scene become important for the criminal investigation process. The clear coat is of great interest since it is most likely to be transferred through contact and therefore found at the crime scene. Fourier transform infrared spectroscopy with attenuated total reflectance (ATR-FTIR) spectroscopy combined with chemometric tools is shown to efficient for forensic examination of clear coats. The possibility of improving the chemometric model generalizability was shown through a dataset encompassing diverse 125 samples. The chemical signatures of melamine cross‐linking and urethane were observed during investigation of spectral data of individual clear coats. The best exploratory principal components analysis (PCA) model was constructed with the first four principal components (PCs), that described 90.3 % of the total data variance. Supervised model classification using partial least squares – discriminant analysis (PLS-DA) showed ssensitivity (SEN) and specificity (SPC) of 95 % and 100 % for external test set (overall accuracy was 92 %). Independent components analysis (ICA) model with 6 independent components (ICs) was used to interpret chemical features responsible for class separation. High predictive ability and interpretability of multivariate approach demonstrates the potential of ATR-FTIR spectroscopy and chemometrics for characterization of automotive clear coats of various manufacturers and models.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100563"},"PeriodicalIF":3.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancement in electrochemical sensing of chloramphenicol in varying matrixes: A review 氯霉素在不同基质中的电化学传感研究进展

IF 3.7

Talanta Open Pub Date : 2025-09-20 DOI: 10.1016/j.talo.2025.100561

Kgwadu Percy Mulaudji, Kefilwe Vanessa Mokwebo, Franklin Quelain De Bruin, Keagan Pokpas, Natasha Ross

{"title":"Advancement in electrochemical sensing of chloramphenicol in varying matrixes: A review","authors":"Kgwadu Percy Mulaudji, Kefilwe Vanessa Mokwebo, Franklin Quelain De Bruin, Keagan Pokpas, Natasha Ross","doi":"10.1016/j.talo.2025.100561","DOIUrl":"10.1016/j.talo.2025.100561","url":null,"abstract":"<div><div>Chloramphenicol (CAP) have demonstrated its broad effectiveness against bacterial infections. However, its persistence in the environment and potential toxicity led to strict global regulations limiting its use. As a result, rapid and accurate detection methods for CAP are being developed to protect public health and maintain regulatory compliance regarding its presence in the environment. Conventional analytical methods such as High-Performance Liquid Chromatography, Gas Chromatography, and Liquid Chromatography–Mass Spectrometry were commonly used for CAP detection. However, these conventional methods suffer challenges such as time-consumption, fabrication complexity, reproducibility, and cost. With electrochemical sensing techniques presenting varying valuable benefits, such as instant detection, low power consumption, simultaneous analysis, and portability, making them essential for timely monitoring of various analytes. The performance of electrochemical sensor is further boosted by integrating materials such as spinels, metal oxides, and metallic nanoparticles. These materials are broadly investigated as electrode interface components thanks to their features that collectively boost electrochemical sensor such as providing excellent electrical conductivity, distinct catalytic behavior, and larger surface areas. Furthermore, this paper reviews the impact of traditional and standards analytical methods, as well as their recent advancement based on the detection of CAP in aqueous media.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100561"},"PeriodicalIF":3.7,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness 环保高效液相色谱法同时测定泮托拉唑和多潘立酮的绿、白、蓝综合评价

IF 3.7

Talanta Open Pub Date : 2025-09-15 DOI: 10.1016/j.talo.2025.100560

Aya Barseem , Reem H. Obaydo , Samar H. Elagamy

{"title":"Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness","authors":"Aya Barseem , Reem H. Obaydo , Samar H. Elagamy","doi":"10.1016/j.talo.2025.100560","DOIUrl":"10.1016/j.talo.2025.100560","url":null,"abstract":"<div><div>An environmentally friendly RP-HPLC method was developed for the simultaneous determination of pantoprazole (PAN) and domperidone (DOM). Chromatographic separation was performed using isocratic elution on CORTECS® Shield RP18 column maintained at 25 °C. The mobile phase consisted of ethanol and potassium phosphate buffer (50.0 mM, pH 6.0) in a 30:70 (v/v) ratio, delivered at a flow rate of 1.0 mL/min, with UV detection at 286 nm. The method demonstrated excellent linearity within the concentration ranges of 1.0–25.0 μg/mL for PAN and 3.0–30.0 μg/mL for DOM. It was successfully applied to the analysis of both drugs in bulk and pharmaceutical dosage forms. The greenness of the proposed method was evaluated using the Analytical Eco-Scale, yielding a total score of 83, which indicates minimal environmental impact. The method \"whiteness\" was assessed using the RGB 12 model, resulting in a high total score of 89.2, reflecting a well-balanced combination of analytical performance, environmental sustainability, and practical implementation. Its \"blueness,\" representing practical applicability, was measured using the BAGI tool and achieved a total score of 82.5. Compared to a previously reported HPLC method, the proposed method offers comparable practical applicability while exhibiting enhanced greenness through the use of ethanol as the organic modifier, rather than environmentally hazardous solvents such as acetonitrile and methanol. The method was fully validated according to ICH Q2(R1) guidelines, confirming its accuracy, precision, specificity, and robustness. Furthermore, the proposed method was statistically compared with a previously reported HPLC method using Student’s <em>t</em>-test and F-test.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100560"},"PeriodicalIF":3.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers 一种简单、高通量ICP-MS测定尿液中46种元素的方法：汽车工人职业暴露的生物监测

IF 3.7

Talanta Open Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100559

Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen

{"title":"A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers","authors":"Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen","doi":"10.1016/j.talo.2025.100559","DOIUrl":"10.1016/j.talo.2025.100559","url":null,"abstract":"<div><div>Monitoring urinary metal concentrations is crucial for assessing occupational exposure and associated health risks. Inductively coupled plasma mass spectrometry (ICP-MS) is widely considered the method of choice for multi-element analysis due to its high sensitivity, low detection limits, and broad dynamic range. This study presents a simple and rapid ICP-MS method for the simultaneous determination of 46 elements in urine, utilizing 2 % nitric acid and 1.5 % ethanol (v/v) for sample pretreatment. This approach effectively mitigates matrix effects and carbon-based interferences. The method demonstrated excellent linearity (coefficient of determination, R² ≥ 0.9985), precision (relative standard deviation, RSD < 8 %), and accuracy (recoveries 80–120 %). The method was applied to analyze 268 urine samples from automotive manufacturing workers. Several elements of occupational relevance, including Nickel (Ni), tungsten (W), molybdenum (Mo), titanium (Ti) and Cadmium (Cd), as well as essential elements such as iron (Fe), copper (Cu), and zinc (Zn), were elevated compared with general population references, indicating complex multi-metal exposure profiles. This work provides a validated and efficient tool for occupational biomonitoring and delivers valuable elementomic data to support exposure assessment, workplace interventions, and future research on the health effects of multi-metal exposure.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100559"},"PeriodicalIF":3.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical methodologies for antioxidant capacity assessment: An updated review 抗氧化能力评估的分析方法：最新综述

IF 3.7

Talanta Open Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100558

Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour

{"title":"Analytical methodologies for antioxidant capacity assessment: An updated review","authors":"Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour","doi":"10.1016/j.talo.2025.100558","DOIUrl":"10.1016/j.talo.2025.100558","url":null,"abstract":"<div><div>Antioxidants are crucial molecules protecting biological systems from harmful oxidation reactions and free radicals. This class of compounds attracted remarkable attention for their potential in functional foods and dietary supplements. The imbalance between oxidants and antioxidants leads to oxidative stress, associated with various chronic diseases. Accurate measurement of antioxidant activity is therefore essential for evaluating potential health-enhancing agents in food science, medicine, and biotechnology. This review provides a comprehensive overview of contemporary analytical methodologies for assessing antioxidant capacity across food, cosmetic, pharmaceutical, and biomedical applications, exploring four major categories of techniques: spectrophotometric methods, including UV–Vis, electron paramagnetic resonance (EPR), and near infrared (NIR) spectroscopy, highlighting their principles and applications in radical scavenging assays and antioxidant characterization. Electrochemical techniques are rapid, sensitive, and cost-effective alternatives, focusing on voltammetry, amperometry, and biosensor development for direct measurement of electron transfer processes. The role of biosensors, employing bioreceptors like enzymes and DNA, for selective and sensitive antioxidant detection is also examined. Chromatographic methods, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), paired with selective detectors, are used to separate, identify, and quantify antioxidant compounds in complex matrices. This review highlights the principles, advancements, advantages, and limitations of these diverse methodologies in the accurate evaluation of antioxidant potential.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100558"},"PeriodicalIF":3.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0