Talanta Open最新文献

{"title":"Artificial intelligence in chromatography: Greenness and performance evaluation of AI-predicted and in-lab optimized HPLC methods for simultaneous separation of amlodipine, hydrochlorothiazide, and candesartan","authors":"Hayam Mahmoud Lotfy ,&nbsp;Nevin Erk ,&nbsp;Asena Ayse Genc ,&nbsp;Reem Hasan Obaydo ,&nbsp;Gizem Tiris","doi":"10.1016/j.talo.2025.100473","DOIUrl":"10.1016/j.talo.2025.100473","url":null,"abstract":"<div><div>Artificial Intelligence (AI) is revolutionizing analytical chemistry by promising rapid method development, yet its real-world efficacy remains untested in complex pharmaceutical separations. This study leverages AI to design an HPLC method for Amlodipine (AMD), Hydrochlorothiazide (HYD), and Candesartan (CND), comparing it with an experimentally optimized approach to uncover practical benefits and limitations. Our findings reveal AI’s potential to accelerate innovation while highlighting the critical role of human expertise. The In-Lab optimized HPLC method utilized an Xselect CSH Phenyl Hexyl® (2.5 µm, 4.6 × 150 mm) column with a mobile phase of acetonitrile:water (0.1 % trifluoroacetic acid) (70:30, v/v), a flow rate of 1.3 mL/min, and UV detection at 250 nm. It achieved rapid elution with retention times of AMD = 0.95 min, HYD = 1.36 min, and CND = 2.82 min. The AI-generated method used a C18 column (5 µm, 150 mm × 4.6 mm), gradient elution with phosphate buffer (pH 3.0) and acetonitrile, a flow rate of 1.0 mL/min, and detection at 240 nm, resulting in longer retention times: AMD = 7.12 min, HYD = 3.98 min, and CND = 12.12 min. Linearity ranges were AMD (25.0–250.0 µg/mL), HYD (31.2–287.0µg/mL), and CND (40.0–340.0µg/mL) for the In-Lab method, and AMD (30.0–250.0µg/mL), HYD (35.0–285.0µg/mL), and CND (50.0–340.0 µg/mL) for the AI-HPLC method. Both approaches were validated per ICH guidelines, confirming specificity, accuracy, and reliability. The obtained results were statistically compared with the reported ones using the F-test and Student’s t-test. In terms of sustainability, the In-Lab method outperformed the AI-based method according to MoGAPI, AGREE, and BAGI assessments, due to reduced solvent use, waste generation, and analysis time. This study underscores the necessity of human intervention to refine AI-generated methods, aligning them with both analytical efficiency and green chemistry goals. Improving AI tools to predict optimal HPLC conditions is essential for advancing sustainable and effective analytical practices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100473"},"PeriodicalIF":4.1,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable validated methodology for enzymeless determination of Tyrosine using 3D-printed PLA/CB electrodes in synthetic urine","authors":"Amanda Morais ,&nbsp;Diego Pessoa Rocha ,&nbsp;Maria Lurdes Felsner ,&nbsp;Andressa Galli","doi":"10.1016/j.talo.2025.100469","DOIUrl":"10.1016/j.talo.2025.100469","url":null,"abstract":"<div><div>Tyrosine (TYR), an amino acid, is a biomarker for several diseases, making its quantification in biological samples clinically relevant. However, conventional gold-standard methods typically require expensive reagents, are time-consuming, and are often not portable. In addition, in most cases, TYR assays are not validated. To circumvent these inconveniences, we present a validated and sustainable methodology for quantifying TYR in synthetic urine (proof-of-concept) on a Table 3D-printed platform. This work evaluated and achieved promising results in the use of 3D printing in the development of a miniaturized and portable analytical method, which explored aspects of analytical validation. The proposed method was optimized for square wave voltammetry parameters and better background electrolytes, resulting in the following optimized conditions: 8 Hz frequency, 50 mV modulation amplitude, 6 mV pulse increment, and sodium acetate buffer (pH 4.5). Validation, performed according to EURACHEM, IUPAC, and ICH guidelines, evaluated key performance indicators such as selectivity (via matrix effects and Youden’s calibration study), linearity, limits of detection and quantification, precision, and trueness. The method demonstrated adequate selectivity and linearity over the working range of 2 to 10 µmol <em>L</em>^-1 (<em>p</em> &gt; 0.05). At 6 µmol <em>L</em>^−¹, it showed good repeatability (<em>n</em> = 6) and intermediate precision (<em>n</em> = 12), with an RSD (%) &lt;16 %. Recovery tests evaluated trueness using spiked samples at 2, 6, and 10 µmol <em>L</em>⁻¹ (<em>n</em> = 3), with recoveries below 106 %. In addition, the proposed method achieved a high greenness score of 0.79, calculated using the AGREE software, where higher scores indicate more environmentally friendly methods. Overall, the proposed method is a low-cost, sustainable, environmentally friendly, portable, and accessible approach for TYR analysis in biological samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100469"},"PeriodicalIF":4.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pigment-based biosensors for environmental monitoring","authors":"Shivangini Desai,&nbsp;Sonal Desai","doi":"10.1016/j.talo.2025.100471","DOIUrl":"10.1016/j.talo.2025.100471","url":null,"abstract":"<div><div>Environmental pollution is progressively escalating owing to an increase in population and industrialization. Various industrial wastes are discharged into the water, soil, and air. Consequently, there is an urgent need to monitor the levels of these pollutants in the environment, as they pose a threat not only to human health, but also to the ecosystem, affecting all organisms. A biosensor detects biological or chemical processes by generating signals proportional to the analyte concentration in the reaction. Biosensors are utilized to monitor Biochemical Oxygen Demand (BOD), pesticides, phenols, heavy metals, and polluting gases. Pigment-based biosensors facilitate colorimetric and visual detection without the need for complex instrumentation. These biosensors can produce a response either because the analyte reacts directly with the pigment or through the analyte interacting with a genetic pathway that leads to pigment production, resulting in colour change. Through the genetic engineering of microorganisms to produce specific pigments, specific heavy metal detection can be achieved. This study examines the applications of pigment-based biosensors in the environmental monitoring of various pollutants, including heavy metals and pesticides, in contrast to conventional detection techniques. It also explores the recent advancements and limitations.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100471"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantification of oleanolic acid and apigenin in biological matrices following intraperitoneal administration of co-loaded nanoformulations: Method validation and biodistribution studies","authors":"Amna Naz ,&nbsp;Sadia Haider ,&nbsp;Mavra Rana ,&nbsp;Neelum Malik ,&nbsp;Attia Afzal ,&nbsp;Muhammad Farooq ,&nbsp;Muhammad Sarfraz","doi":"10.1016/j.talo.2025.100470","DOIUrl":"10.1016/j.talo.2025.100470","url":null,"abstract":"<div><div>Oleanolic acid (OA) and apigenin (APG) demonstrate a wide range of pharmacological effects, including antioxidant, anti-inflammatory, antiviral, antidiabetic, hepatoprotective, and cardioprotective activities. Both compounds show anticancer potential and have been used individually to treat various cancer types, creating a rationale for their combined use. However, quantifying both drugs in quality and plasma samples presents challenges due to differences in their physicochemical properties and absorption wavelengths (OA: 210–215 nm, APG: 212, 269, and 337 nm). We developed and validated a novel High-Performance Liquid Chromatography UV detection (HPLC-UV) method to address this problem. Using a C18 column (4.6 mm x 250 mm, packing L1, 5 µm particle size) and a mobile phase of acetonitrile: methanol: tetrahydrofuran: water (60:20:8:12, v/v) with a detection wavelength of 215 nm. The method achieved retention times of 4.7 and 10 minutes for OA and APG in quality samples, and 4.9 and 9.8 minutes in plasma samples, respectively. The plasma sample was prepared by simple protein precipitation step. All validation parameters, including sensitivity, selectivity, precision, accuracy, robustness, and stability, met the required criteria. The validated HPLC-UV bioanalytical method was effectively applied in quantification, stability, and pharmacokinetic studies of co-loaded nanoformulations of OA and APG with particle size 163 nm. The entrapment efficiency for OA was 93.95 ± 3.50 %, and for APG, it was 94.5 ± 2.31 % in combined nanoformulation, establishing it as a promising analytical technique for detecting these anticancer agents in complex matrices such as blood, plasma, and polymeric systems.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100470"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A facile and rapid detection of Cr(VI) and ascorbic acid using N,S-co-functionalized carbon dots: A dual-mode 'on-off-on' fluorescent sensor","authors":"D. James Nelson ,&nbsp;N.S.K. Gowthaman ,&nbsp;Bharathi Sinduja ,&nbsp;M.G. Sethuraman","doi":"10.1016/j.talo.2025.100472","DOIUrl":"10.1016/j.talo.2025.100472","url":null,"abstract":"<div><div>This study reports the successful synthesis of nitrogen- and sulfur-co-functionalized carbon dots (N,S-CDs) using citric acid and L-cysteine through a microwave-assisted hydrothermal process. These N,S-CDs were employed for the quantification of dichromate ions (Cr(VI)) and ascorbic acid (AA) through a fluorescence “on-off-on” detection method. The N,S-CDs displayed deep blue fluorescence, with an emission peak at 420 nm upon excitation at 349 nm. The presence of Cr(VI) significantly quenched the fluorescence intensity, enabling its selective detection over a linear range of 1–40 μM with a limit of detection of 58 nM. The addition of AA restored the fluorescence intensity by reducing Cr(VI) to Cr(III), allowing for AA detection within a dynamic range of 5–90 μM with a detection limit of 180 nM. The fluorescence quenching by Cr(VI) involved both the inner filter effect (IFE) and static interactions and the IFE was reversed upon the introduction of AA, which restored the fluorescence. This method demonstrated excellent sensitivity and selectivity for detecting Cr(VI) and AA, with successful application in the analysis of real samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100472"},"PeriodicalIF":4.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel ISE Modified with Synthesized Magnetite Nanoparticles for Sulfates Determination","authors":"Ita Hajdin ,&nbsp;Karmela Zanki Kulazo ,&nbsp;Ante Prkić ,&nbsp;Kristian Nakić ,&nbsp;Andrea Paut ,&nbsp;Lucija Guć ,&nbsp;Mitja Kolar ,&nbsp;Boštjan Genorio ,&nbsp;Ivana Mitar","doi":"10.1016/j.talo.2025.100468","DOIUrl":"10.1016/j.talo.2025.100468","url":null,"abstract":"<div><div>Potentiometric ISEs are one of the fastest developing sensors in electrochemistry due to their wide range of applications. They are used in the food industry, agriculture, environmental and drug analysis as they offer numerous advantages: high selectivity and sensitivity, low LOD, small size, easy handling, portability and low cost. We have therefore developed a solid-state ISE for the potentiometric determination of sulfate ions. The membranes are based on a mixture of barium sulfate, silver sulfide, polymer and metal oxide nanoparticles. The mass ratio of BaSO₄, Ag₂S and PTFE in the membranes was 1 : 1 : 2, with the addition of various metal oxide NPs, such as Fe₃O₄, α-Fe₂O₃, Al₂O₃ or ZnO, to the membrane composition. The membrane containing magnetite NPs showed a positive effect on the linear response range for sulfate anions. In particular, the membrane containing 0.6% magnetite NPs showed a linear potential change with a slope of 28.5 mV dec⁻¹, which is very close to the theoretical Nernstian value (29.6 mV dec⁻¹), with a regression coefficient of 0.9978 in the concentration range between 4.88 ∙ 10⁻⁵ mol L⁻¹ and 1.00 ⸱ 10⁻² mol L⁻¹ with LOD 4.40 ∙ 10⁻⁵ mol L⁻¹. Finally, none of the membranes showed response to barium ions even at high concentrations (≥ 1.00 ∙ 10⁻³ mol L⁻¹). These results confirm that our newly developed potentiometric sensor has a high membrane selectivity for the determination of sulfate ions and, most importantly, that it is a fast, reliable and cost-effective tool.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100468"},"PeriodicalIF":4.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nature-driven blue-emissive N, S-CDs: Harnessing sequential “switch-off-on” fluorescence signals for detection of chrysin and Al³⁺ along with cellular imaging versatility","authors":"Maha Mohammad Abdel-Monem ,&nbsp;Mohamed I. Walash ,&nbsp;Asmaa Kamal El-Deen","doi":"10.1016/j.talo.2025.100466","DOIUrl":"10.1016/j.talo.2025.100466","url":null,"abstract":"<div><div>Novel biocompatible blue emissive nitrogen and sulfur co-doped carbon dots (N, S-CDs) were synthesized <em>via in situ</em> microwave-assisted synthesis from snake melon (<em>Cucumis melo</em> var. <em>flexuosus</em>) for the first time. The N, S-CDs have been utilized for fluorimetric determination of chrysin (CHR). The fluorescence emitted by the N, S-CDs (λ_em = 460 nm) decreased upon the addition of CHR due to dynamic quenching and the inner filter effect (IFE). The reduction in emission intensity exhibits a linear relationship over a concentration range of 2.0–275.0 μM with a low limit of detection (LOD) of 0.0837 μM. By contrast, the quenched fluorescence of the N, S-CD/CHR system was restored upon the addition of Al³⁺, which efficiently displaced CHR from the surface of the N, S-CDs, prompting fluorescence \"switch-on\". The retrieved fluorescence intensity was proportional to Al³⁺ concentration over a range of 50.0–4000.0 μM, with a LOD of 2.96 μM. This sequential approach was efficiently utilized to determine CHR and Al³⁺ in real pharmaceutical, biological, and environmental samples with accurate and precise findings. Furthermore, due to their minimal cytotoxicity, the N, S-CDs were also successful for cellular imaging on HepG2 cells. The proposed sensing platform provides several merits, including simplicity, cost-efficiency, long-lasting stability, high selectivity, and exceptional sensitivity. These findings not only contribute to the growing field of carbon-based nanomaterials but also offer a sustainable and multifunctional platform for environmental and biomedical applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100466"},"PeriodicalIF":4.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Zein-templated gold nanoclusters and their application as a fluorescent probe for tetracycline detection","authors":"Yun Bai ,&nbsp;Gerile Aodeng ,&nbsp;Jun Ai ,&nbsp;Guirong Borijihan","doi":"10.1016/j.talo.2025.100467","DOIUrl":"10.1016/j.talo.2025.100467","url":null,"abstract":"<div><div>Gold nanoclusters (Au NCs) are a special type of gold nanomaterial containing multiple gold atoms. Compared to other fluorescent materials, Au NCs exhibit stronger stability and longer fluorescence lifetimes. In the synthesis of Au NCs, plant proteins offer significant advantages over animal or microbial proteins. Plant proteins are abundant, cost-effective, sustainable, and hypoallergenic. Therefore, in this study, we used Zein, a corn protein, as a template to prepare Zein-templated Au NCs using a water bath method. The optical properties of Zein-Au NCs were characterized by fluorescence spectrophotometry and UV–Vis spectrophotometry, while the morphology of the nanoclusters was examined by transmission electron microscopy. When excited at 410 nm, the maximum emission wavelength of Zein-Au NCs was observed at 490 nm. After the introduction of tetracycline, the emission peak at 490 nm was enhanced. Therefore, Zein-Au NCs can be used as a fluorescent probe for the detection of tetracycline.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100467"},"PeriodicalIF":4.1,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green analytical chemistry in gabapentin analysis: Methods and sustainability review","authors":"Hemraj Sharma ,&nbsp;Kanaka Parvathi Kannaiah ,&nbsp;Reem Hasan Obaydo ,&nbsp;Rita Keshishian ,&nbsp;Hemanth Kumar Chanduluru ,&nbsp;Kritika Bhattarai ,&nbsp;Ashish Acharya","doi":"10.1016/j.talo.2025.100465","DOIUrl":"10.1016/j.talo.2025.100465","url":null,"abstract":"<div><div>Gabapentin (GP), widely prescribed for seizures and neuropathic pain, requires effective therapeutic monitoring and pharmaceutical quality control. Current analytical methods, such as UV spectrophotometry, HPLC, GC–MS, and LC-MS/MS, often rely on environmentally hazardous solvents and high-energy processes. This review evaluates analytical methods for GP till 2024, focusing on their eco-friendliness using green analytical chemistry (GAC) metrics. The study highlights the lack of concern for solvent toxicity, energy consumption, and waste generation in existing methodologies. Greenness assessment tools like GAPI, MoGAPI, AGREE, BAGI, AES reveal significant gaps in sustainability. This review underscores the need to develop greener analytical techniques for GP and advocate for eco-friendly solvents, reduced waste, and optimized energy use. Integrating GAC principles into analytical practices can enhance sustainability and robustness, reducing the environmental footprint of GP analysis. By compiling this information, the review aims to guide researchers in adopting innovative, eco-conscious methodologies, and aligning analytical procedures with sustainable chemistry goals.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100465"},"PeriodicalIF":4.1,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selectivity towards methyl mercaptan of Pt-PtO functionalized WO3 nanostructures made by flame spray pyrolysis","authors":"Kanittha Inyawilert ,&nbsp;Matawee Punginsang ,&nbsp;Chonlada Dechakiatkrai Theerakarunwong ,&nbsp;Anurat Wisitsoraat ,&nbsp;Chaikarn Liewhiran","doi":"10.1016/j.talo.2025.100464","DOIUrl":"10.1016/j.talo.2025.100464","url":null,"abstract":"<div><div>This work presented 0–1 wt% Pt-PtO functionalized WO₃ nanostructures synthesized by flame spray pyrolysis (FSP) for methyl mercaptan (CH₃SH)-sensing applications. According to electron microscopic and X-ray spectroscopic analysis, 1–3 nm Pt-PtO core-shell nanoparticles were decorated on 5–20 nm primary monoclinic WO₃ nanoparticles. The gas-sensing data revealed that Pt-PtO loading with an optimum Pt content of 0.5 wt% provided a remarkably enhanced CH₃SH response of 204 to 500 ppb CH₃SH at the best sensing temperature of 250°C with a low detection limit of 26 ppb and high CH₃SH selectivity relative to (CH₃)₂S, H₂S, CH₃OH, C₂H₅OH, C₃H₆O, NH₃, NO₂, H₂, CH₄, C₂H₂ and short-chain fatty acids (SCFAs) including CH₃COOH, C₂H₅COOH, C₃H₇COOH. Therefore, the flame-synthesized Pt-PtO functionalized WO₃ nanosensors could be potential for CH₃SH detection at ppb levels in the medical applications particularly noninvasive breath diagnosis of halitosis and serious infection of periodontitis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100464"},"PeriodicalIF":4.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}