Talanta Open最新文献

{"title":"Development, validation, and verification of the gas chromatography-mas spectrometry method for detecting and quantifying major cannabinoids in the inflorescence of Cannabis sativa L","authors":"Saša D. Đurović ,&nbsp;Nemanja Brkljača ,&nbsp;Zorana Srećkov ,&nbsp;Zorica Mrkonjić ,&nbsp;Dejana Panković","doi":"10.1016/j.talo.2025.100455","DOIUrl":"10.1016/j.talo.2025.100455","url":null,"abstract":"<div><div><em>Cannabis sativa</em> L. is one the most intriguing plants these days. This is mainly because of the cannabinoids that are synthesized during maturation. Two main cannabinoids are cannabidiol (CBD) and Δ⁹-tetrahydrocannabinol (THC), followed by cannabigerol (CBG) and cannabinol (CBN). The CBD, THC, and CBN ratio determines whether it is the drug-type or fiber-type of the plant. We must have a precise and accurate sample preparation and analysis method to determine which it is. Several techniques are used for the analysis of cannabinoids. Among them is gas chromatography coupled with a flame ionization detector and/or mass spectrometer. To avoid the problem with elevated temperatures (decarboxylation, oxidation, and isomerization), decarboxylation was performed prior to the analysis, and tribenzylamine (TBA) was used as an internal standard. The first step was creating the calibration curves for CBD, THC, CBG, and CBN. After that, three hemp varieties (Helena, Marina, and Fedora 17) were used to validate and verify the method. High precision with intra-day and inter-day precisions (low coefficients of variation, CV) and accuracy for both standards and spiked samples proved the method's successful development, validation, and verification. The absence of CBN and Δ⁸-THC in plant samples showed that decarboxylation could be successfully applied, avoiding oxidation or isomerization of THC. Application of TBA showed that there is no need for deuterated standards. Quantitative analysis showed that analyzed strains are fiber-type plants and that the method developed in this study may be used to analyze cannabinoid content in plant samples accurately.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100455"},"PeriodicalIF":4.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143850204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A nanolevel sustainable fluorometric approach employing Eosin Y, a biological dye, as an innovative quenching-based biochemical sensor for linagliptin determination","authors":"Mohamed A. El Hamd ,&nbsp;Badriah Saad Al-Farhan ,&nbsp;Safaa F. Saleh ,&nbsp;Wael A. Mahdi ,&nbsp;Sultan Alshehri ,&nbsp;Bandar R. Alsehli ,&nbsp;Ahmed Abdulhafez Hamad","doi":"10.1016/j.talo.2025.100452","DOIUrl":"10.1016/j.talo.2025.100452","url":null,"abstract":"<div><div>A new spectrofluorimetric method was devised to enable the accurate and eco-friendly determination of Linagliptin (LGT), a pharmaceutical agent recognized for its anti-diabetic properties. This investigation introduced a reliable, highly sensitive, and environmentally friendly analytical protocol for the examination and authentication of this therapeutic compound. The approach harnessed the swift electrostatic forces that arise between Eosin Y, an approved non-toxic biological stain, and LGT under mildly acidic conditions. This interaction resulted in a notable quenching of fluorescence for Eosin Y, predominantly observed at an emission wavelength of 557 nm. due to the formation of LGT-EY quenching bio-sensor. The method demonstrated exceptional analytical parameters, including limits of detection and quantification set at 23.3 ng mL^-1 and 70.7 ng mL^-1, respectively, within a calibration range spanning 100.0–1400.0 ng mL^-1. Comprehensive optimization was performed to refine the factors influencing the interaction mechanism between the analyte and the dye. Detailed validation processes confirmed adherence to the International Council for Harmonization (ICH) guidelines, substantiating the procedure's precision and accuracy. The newly established method was successfully applied to measure LGT in its bulk form, formulated products, and various complex matrices. From a sustainability perspective, the procedure underwent a thorough evaluation using advanced tools aligned with green chemistry principles. Under the White Analytical Chemistry (WAC) framework, the assessment employing the RGB 12 model categorized the method as \"white,\" indicative of its optimal environmental and operational efficiency. Further analysis using the new BAGI metric reinforced the method's sustainability and versatility by yielding high ratings in both dimensions, underscoring its reliability and extensive applicability.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100452"},"PeriodicalIF":4.1,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liquid chromatography tandem mass spectrometry method for the quantification of total and free ropivacaine in human plasma","authors":"Amedeo De Nicolò ,&nbsp;Alessandra Manca ,&nbsp;Edoardo Ceraolo ,&nbsp;Giulio Luca Rosboch ,&nbsp;Antonio Toscano ,&nbsp;Luca Neitzert ,&nbsp;Eleonora Balzani ,&nbsp;Jessica Cusato ,&nbsp;Alice Palermiti ,&nbsp;Giorgia Montrucchio ,&nbsp;Antonio D'Avolio","doi":"10.1016/j.talo.2025.100453","DOIUrl":"10.1016/j.talo.2025.100453","url":null,"abstract":"<div><div>Ropivacaine, a local anaesthetic with a convenient pharmacokinetic/pharmacodynamic profile, deserved attention for several applications. Despite a good safety profile, this drug can still be associated with systemic toxicity, due to erroneous site of injection or due too fast absorption or low volume of distribution. Moreover, ropivacaine is normally bound to α1-acid glycoprotein, and low concentrations of this transport protein can lead to higher-than-normal free concentrations. Since the description of total and free concentrations of Ropivacaine is pivotal to understand the probability of local anaesthetic systemic toxicity (LAST) and the underlying reasons, in this work we developed and validated a simple method for their quantification in human plasma.</div><div>Plasma samples underwent protein precipitation for the determination of total Ropivacaine concentrations and an unltrafiltration process with Centrifree® filters, to isolate the free concentration. Reverse phase separation was obtained with a gradient run of water and acetonitrile:methanol 60:40, both added with 0.1 % formic acid, on a Kinetex® biphenyl 2.1 × 100 mm, 2.6 um column. Quantification was performed in ESI+ MRM mode and internal standardization with Stable-Isotope-Linked Ropivacaine.</div><div>The method was validated following EMA ICH guidelines and showed satisfactory performance in terms of accuracy and precision (both bias and coefficient of variation) lower than 10 %, linearity, matrix effect, recovery, sensitivity and specificity. Ultrafiltration was not associated with drug loss due to adsorption to the molecular filters.</div><div>Finally, the method was applied on samples from patients enrolled in a clinical study, confirming its clinical suitability for the analysis of Ropivacaine in human plasma.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100453"},"PeriodicalIF":4.1,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of 3D printing conditions for BaCe0.6Zr0.3Y0.1O3-δ in the construction of amperometric high-temperature H2 sensors","authors":"Antonio Hinojo,&nbsp;Enric Lujan,&nbsp;Sergi Colominas,&nbsp;Jordi Abella","doi":"10.1016/j.talo.2025.100454","DOIUrl":"10.1016/j.talo.2025.100454","url":null,"abstract":"<div><div>Proton-conducting ceramics, particularly BaCe_0.6Zr_0.3Y_0.1O_3-δ (BCZY), are promising materials for hydrogen energy applications. However, traditional fabrication methods are limited in their ability to produce complex geometries. Extrusion-based 3D printing presents a promising alternative, enabling the fabrication of customized designs with the advantage of fast prototyping. This study optimized the slurry composition and 3D printing parameters for BCZY ceramics to fabricate pellets and one-end closed tubes for amperometric hydrogen sensors. Results showed that a paste with 83 % BCZY, 8.5 % water, and 8.5 % PEG400 yielded a density of 96 % after sintering at 1700 °C for 1 h. The nozzle diameter during printing was the most influential parameter affecting wall thickness. Sensors constructed from one-end closed tubes exhibited higher sensitivity (20,571 μm·mbar^-1) and a broader linear range (0.010 - 0.050 mbar) compared to pellet-based sensors. 3D printing proves to be an effective method for producing BCZY ceramic components with tailored geometries for hydrogen sensing.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100454"},"PeriodicalIF":4.1,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143823315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering europium nanoparticle-HIV-1 p24 conjugates as a novel platform for rapid and sensitive antibody detection","authors":"Aditya Kurdekar ,&nbsp;Sai Manohar Chelli ,&nbsp;Mohan Kumar Haleyur Giri Setty ,&nbsp;Indira K. Hewlett ,&nbsp;Venkataramaniah Kamisetti","doi":"10.1016/j.talo.2025.100451","DOIUrl":"10.1016/j.talo.2025.100451","url":null,"abstract":"<div><div>The detection and quantification of immune markers in biological samples play a critical role in various fields, including diagnostics, biomedical research, and therapeutics. Western blotting or enzyme-linked immunosorbent assays (ELISAs), which are labour-intensive and call for complicated processes, are frequently used in traditional immunodetection techniques. However, fluorescent nanoparticle-antigen conjugates have become an innovative method for immunogenic detection thanks to the recent developments in nanotechnology. This study demonstrates the fabrication and working principle of conjugates of fluorescent nanoparticles and HIV-1 p24 in antibody detection. Europium nanoparticles are the fluorophore conjugated to the HIV-1 p24 antigen, a capsid protein. Computational studies were conducted to determine whether the bioconjugation would be feasible, and the findings showed high stability of the conjugates. After validation, the bioconjugation of HIV-1 p24 and the nanoparticle was carried out using the EDC-NHS approach. Studies employing anti-HIV-1 p24 antibodies demonstrated that Europium nanoparticle-p24 (EuNP-p24) conjugates could achieve a sub-picogram-level sensitivity of 0.4 pg/mL and a very high recovery rate of ≥92 %, indicating their potential for improving the sensitivity of the assay and its efficiency in clinical diagnostics.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100451"},"PeriodicalIF":4.1,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a non-derivatized LC-MS/MS method for simultaneous quantification of ten neurotransmitters in five neuroinflammatory mouse models","authors":"Bao Sun ,&nbsp;Zhaodi Xia ,&nbsp;Chen Wang ,&nbsp;Mengyao Wu ,&nbsp;Ru Tie ,&nbsp;Yilin Ru ,&nbsp;Yaxi Meng ,&nbsp;Lu Bai ,&nbsp;Ying Zhang ,&nbsp;Pu Jia ,&nbsp;Shixiang Wang ,&nbsp;Bing Qi ,&nbsp;Xiaohui Zheng","doi":"10.1016/j.talo.2025.100449","DOIUrl":"10.1016/j.talo.2025.100449","url":null,"abstract":"<div><div>Sensitive and comprehensive measurement of systemic metabolites of Trp, Phe and Glu in biological samples is effective for understanding the pathogenesis of pain, anxiety, depression, PTSD and other neurological diseases. This study developed a LC-MS/MS method for simultaneous monitoring the 10 neurotransmitters in mouse hippocampus, and applied it to investigate their changes in five neuroinflammatory mouse models. The quantitative analysis of 10 neurotransmitters, including Gamma-aminobutyric acid (γ-GABA), l-Glutamate (Glu), l-Glutamine (Gln), l-Tryptophan (Trp), Serotonin (5-HT), 5-Hydroxyindoleacetic acid (5-HIAA), l-Phenylalanine (Phe), l-Tyrosine (L-Tyr), Dopamine (DA), Levodopa (L-DOPA) was achieved within a chromatographic separation time of 4.5 min by the application of ThermoFisher Scientific™ Vanquish™ HPLC coupled to ThermoFisher ScientificTM TSQ QuantisTM mass spectrometer. Optimized chromatographic separation as well as high sensitivity allow the simultaneous analysis and precise quantification of ten neurotransmitters in mouse hippocampus. The limits of detection in sample ranged from 0.3pg (Phe) to 0.1 μg (γ-GABA). Recoveries were between 88.0 to 113.8 %, the mean intra - day and inter - day coefficient of variation of 5.56 % and 4.72 %, respectively. The matrix effect was from 85.1 to 114.9 %. A series of significant changes in five neuroinflammatory mouse models can be detected. γ-GABA, Glu, Gln, DA, Phe, l-Tyr, 5-HIAA were decreased in depression model. Gln and Glu were decreased in PTSD model. 5-HIAA was elevated in anxiety. 5-HIAA and l-Trp were decreased in CFA and SNI model, respectively. In conclusion, a LC-MS/MS method for simultaneous measurement of 10 neurotransmitters in mouse hippocampus was developed and successfully applied to investigation of the changes in five neuroinflammatory mouse models. The method would be expected to provide applicability to the study of the mechanisms of neurological diseases associated with these neurotransmitters.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100449"},"PeriodicalIF":4.1,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sample preparation in analytical peptidomics: It's all about timing","authors":"Amélie Descamps ,&nbsp;Bart De Spiegeleer ,&nbsp;Evelien Wynendaele ,&nbsp;Petar Naumovski ,&nbsp;Anton De Spiegeleer","doi":"10.1016/j.talo.2025.100450","DOIUrl":"10.1016/j.talo.2025.100450","url":null,"abstract":"<div><div>Identifying reliable biomarkers for rapid and accurate disease detection, prognosis, and treatment is becoming increasingly important. Peptides have recently emerged as a promising group in this domain. However, detailed information about their short-term analytical stability remains limited, from sample collection to the obtention of stabilized aliquots for UPLC-MS/MS analysis. This study assessed the impact of time-dependent sample preparation from blood and saliva on the stability of 17 structurally diverse quorum sensing peptides, a class of biologically active peptides. A significant time effect was observed, defined as the percentage ratio of slow versus rapid sample preparation under clinically relevant conditions. The average (± SEM) stability of the investigated peptides was found to be 55 (±19) % in blood and 32 (±22) % in saliva. A more detailed analysis of the kinetic profiles of five selected peptides revealed that metabolization (notably in blood) and adsorption (notably in saliva) were the primary factors influencing the observed time effect. The implications of these findings on detection limits were also discussed. This study highlights the critical importance of precise timings in analytical peptidomics procedures.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100450"},"PeriodicalIF":4.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted polymers and metal-organic framework-based nanomaterial sensors for food and beverage analysis and safety–A review","authors":"Favour Ezinne Ogulewe,&nbsp;Akeem Adeyemi Oladipo,&nbsp;Mustafa Gazi","doi":"10.1016/j.talo.2025.100448","DOIUrl":"10.1016/j.talo.2025.100448","url":null,"abstract":"<div><div>Global health is significantly threatened by foodborne diseases and contaminants, highlighting the need for rapid, cost-effective detection methods. Molecularly imprinted polymers (MIPs) and metal-organic frameworks (MOFs) offer promising solutions, overcoming limitations of traditional analytical methods. This review examines the integration of MIPs and MOFs in electrochemical and fluorescence sensors for food analysis, detailing sensing mechanisms, performance factors, and electrode coating advancements. A significant portion of this review is dedicated to exploring the application of MIP and MOF-based sensors for the detection of a wide array of contaminants in food matrices, encompassing antibiotics, hormones, pesticides, mycotoxins, heavy metals, pathogenic microorganisms, food additives, and allergens. Despite progress, a comprehensive understanding of MIP/MOF-analyte interactions is crucial and remains a critical knowledge gap. This review critically analyses this gap, highlighting the imperative for further research aimed at elucidating these mechanisms to optimize sensor performance.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100448"},"PeriodicalIF":4.1,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative metal-sensitized spectrofluorimetric method for the simultaneous determination of resveratrol and doxorubicin in human plasma: A holistic sustainability appraisal","authors":"Maha Mohammad Abdel-Monem ,&nbsp;Mohamed I. Walash ,&nbsp;Asmaa Kamal El-Deen","doi":"10.1016/j.talo.2025.100447","DOIUrl":"10.1016/j.talo.2025.100447","url":null,"abstract":"<div><div>The potential synergistic cardioprotective effects of resveratrol and doxorubicin combination have made precise analytical approaches necessary for determining their concentrations in human plasma. Herein, we propose a novel spectrofluorimetric method for the concurrent determination of resveratrol and doxorubicin in spiked human plasma using constant-wavelength synchronous fluorescence spectroscopy (CW-SFS) and fluorescence sensitization with metal chelating agent. By taking the advantage of synchronous scan mode, the CW-SFS can simultaneously scan the emission and excitation wavelengths with a fixed wavelength difference (Δλ = 80 nm), eliminating spectral interference and improving selectivity and sensitivity in the process. To further enhance sensitivity, the fluorescence sensitization method with a metal chelating agent was applied, where the fluorescence signal of both drugs is greatly amplified by adding Al³⁺and diluting with ethanol, improving the detection limits. The new approach shows low limits of detection (LODs) and quantification (LOQs), with excellent linearity (<em>R²</em> &gt; 0.999) over a wide concentration range of 10–1000 and 5–700 ng/mL for doxorubicin and resveratrol, respectively. The method was successfully applied to determine both drugs in spiked human plasma along with a holistic sustainability assessment. This innovative analytical method has the potential to be used in therapeutic drug monitoring, thereby facilitating the optimization of doxorubicin and resveratrol combination therapy for enhanced outcomes in therapeutic drug monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100447"},"PeriodicalIF":4.1,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143767173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly molecularly imprinted polymer-based sensing platforms towards pharmaceuticals: recent advances and future prospects","authors":"Rüstem Keçili ,&nbsp;Ghazanfar Hussain ,&nbsp;Chaudhery Mustansar Hussain ,&nbsp;Adil Denizli","doi":"10.1016/j.talo.2025.100446","DOIUrl":"10.1016/j.talo.2025.100446","url":null,"abstract":"<div><div>The gradually growing demand for the sensitive, selective and eco-friendly approaches for pharmaceutical detection has driven significant prograsses in molecularly imprinted polymer (MIP)-based sensing platforms. MIPs, known for their high specificity and robustness, have emerged as promising materials for the design and constrcution of eco-friendly sensing platforms capable of detecting pharmaceuticals in complex matrices. This review paper provides a comprehensive review of recent advances in the design, fabrication and application of eco-friendly MIP-based sensing platforms, with a focus on their eco-friendly and sustainable features and analytical performance. Furthermore, key apprıaches such as the incorporation of green synthesis methods, renewable resources and eco-friendly polymerization techniques are highlighted.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"11 ","pages":"Article 100446"},"PeriodicalIF":4.1,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}