Talanta Open最新文献_第2页

Advancement in electrochemical sensing of chloramphenicol in varying matrixes: A review 氯霉素在不同基质中的电化学传感研究进展

IF 3.7

Talanta Open Pub Date : 2025-09-20 DOI: 10.1016/j.talo.2025.100561

Kgwadu Percy Mulaudji, Kefilwe Vanessa Mokwebo, Franklin Quelain De Bruin, Keagan Pokpas, Natasha Ross

{"title":"Advancement in electrochemical sensing of chloramphenicol in varying matrixes: A review","authors":"Kgwadu Percy Mulaudji, Kefilwe Vanessa Mokwebo, Franklin Quelain De Bruin, Keagan Pokpas, Natasha Ross","doi":"10.1016/j.talo.2025.100561","DOIUrl":"10.1016/j.talo.2025.100561","url":null,"abstract":"<div><div>Chloramphenicol (CAP) have demonstrated its broad effectiveness against bacterial infections. However, its persistence in the environment and potential toxicity led to strict global regulations limiting its use. As a result, rapid and accurate detection methods for CAP are being developed to protect public health and maintain regulatory compliance regarding its presence in the environment. Conventional analytical methods such as High-Performance Liquid Chromatography, Gas Chromatography, and Liquid Chromatography–Mass Spectrometry were commonly used for CAP detection. However, these conventional methods suffer challenges such as time-consumption, fabrication complexity, reproducibility, and cost. With electrochemical sensing techniques presenting varying valuable benefits, such as instant detection, low power consumption, simultaneous analysis, and portability, making them essential for timely monitoring of various analytes. The performance of electrochemical sensor is further boosted by integrating materials such as spinels, metal oxides, and metallic nanoparticles. These materials are broadly investigated as electrode interface components thanks to their features that collectively boost electrochemical sensor such as providing excellent electrical conductivity, distinct catalytic behavior, and larger surface areas. Furthermore, this paper reviews the impact of traditional and standards analytical methods, as well as their recent advancement based on the detection of CAP in aqueous media.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100561"},"PeriodicalIF":3.7,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness 环保高效液相色谱法同时测定泮托拉唑和多潘立酮的绿、白、蓝综合评价

IF 3.7

Talanta Open Pub Date : 2025-09-15 DOI: 10.1016/j.talo.2025.100560

Aya Barseem , Reem H. Obaydo , Samar H. Elagamy

{"title":"Eco-friendly HPLC method for simultaneous determination of pantoprazole and domperidone: Comprehensive evaluation of greenness, whiteness, and blueness","authors":"Aya Barseem , Reem H. Obaydo , Samar H. Elagamy","doi":"10.1016/j.talo.2025.100560","DOIUrl":"10.1016/j.talo.2025.100560","url":null,"abstract":"<div><div>An environmentally friendly RP-HPLC method was developed for the simultaneous determination of pantoprazole (PAN) and domperidone (DOM). Chromatographic separation was performed using isocratic elution on CORTECS® Shield RP18 column maintained at 25 °C. The mobile phase consisted of ethanol and potassium phosphate buffer (50.0 mM, pH 6.0) in a 30:70 (v/v) ratio, delivered at a flow rate of 1.0 mL/min, with UV detection at 286 nm. The method demonstrated excellent linearity within the concentration ranges of 1.0–25.0 μg/mL for PAN and 3.0–30.0 μg/mL for DOM. It was successfully applied to the analysis of both drugs in bulk and pharmaceutical dosage forms. The greenness of the proposed method was evaluated using the Analytical Eco-Scale, yielding a total score of 83, which indicates minimal environmental impact. The method \"whiteness\" was assessed using the RGB 12 model, resulting in a high total score of 89.2, reflecting a well-balanced combination of analytical performance, environmental sustainability, and practical implementation. Its \"blueness,\" representing practical applicability, was measured using the BAGI tool and achieved a total score of 82.5. Compared to a previously reported HPLC method, the proposed method offers comparable practical applicability while exhibiting enhanced greenness through the use of ethanol as the organic modifier, rather than environmentally hazardous solvents such as acetonitrile and methanol. The method was fully validated according to ICH Q2(R1) guidelines, confirming its accuracy, precision, specificity, and robustness. Furthermore, the proposed method was statistically compared with a previously reported HPLC method using Student’s <em>t</em>-test and F-test.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100560"},"PeriodicalIF":3.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers 一种简单、高通量ICP-MS测定尿液中46种元素的方法：汽车工人职业暴露的生物监测

IF 3.7

Talanta Open Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100559

Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen

{"title":"A simple and high-throughput ICP-MS method for the determination of 46 elements in urine: Biomonitoring of occupational exposure in automotive workers","authors":"Ruirui Dai , Huadong Zhang , Wei Yang , Xiaoxi Wang , Xiaoping Li , Yujie Yu , Fengqiong Chen","doi":"10.1016/j.talo.2025.100559","DOIUrl":"10.1016/j.talo.2025.100559","url":null,"abstract":"<div><div>Monitoring urinary metal concentrations is crucial for assessing occupational exposure and associated health risks. Inductively coupled plasma mass spectrometry (ICP-MS) is widely considered the method of choice for multi-element analysis due to its high sensitivity, low detection limits, and broad dynamic range. This study presents a simple and rapid ICP-MS method for the simultaneous determination of 46 elements in urine, utilizing 2 % nitric acid and 1.5 % ethanol (v/v) for sample pretreatment. This approach effectively mitigates matrix effects and carbon-based interferences. The method demonstrated excellent linearity (coefficient of determination, R² ≥ 0.9985), precision (relative standard deviation, RSD < 8 %), and accuracy (recoveries 80–120 %). The method was applied to analyze 268 urine samples from automotive manufacturing workers. Several elements of occupational relevance, including Nickel (Ni), tungsten (W), molybdenum (Mo), titanium (Ti) and Cadmium (Cd), as well as essential elements such as iron (Fe), copper (Cu), and zinc (Zn), were elevated compared with general population references, indicating complex multi-metal exposure profiles. This work provides a validated and efficient tool for occupational biomonitoring and delivers valuable elementomic data to support exposure assessment, workplace interventions, and future research on the health effects of multi-metal exposure.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100559"},"PeriodicalIF":3.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analytical methodologies for antioxidant capacity assessment: An updated review 抗氧化能力评估的分析方法：最新综述

IF 3.7

Talanta Open Pub Date : 2025-09-14 DOI: 10.1016/j.talo.2025.100558

Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour

{"title":"Analytical methodologies for antioxidant capacity assessment: An updated review","authors":"Mahmoud Hamed , Faisal K. Algethami , Alaa Bedair , Fotouh R. Mansour","doi":"10.1016/j.talo.2025.100558","DOIUrl":"10.1016/j.talo.2025.100558","url":null,"abstract":"<div><div>Antioxidants are crucial molecules protecting biological systems from harmful oxidation reactions and free radicals. This class of compounds attracted remarkable attention for their potential in functional foods and dietary supplements. The imbalance between oxidants and antioxidants leads to oxidative stress, associated with various chronic diseases. Accurate measurement of antioxidant activity is therefore essential for evaluating potential health-enhancing agents in food science, medicine, and biotechnology. This review provides a comprehensive overview of contemporary analytical methodologies for assessing antioxidant capacity across food, cosmetic, pharmaceutical, and biomedical applications, exploring four major categories of techniques: spectrophotometric methods, including UV–Vis, electron paramagnetic resonance (EPR), and near infrared (NIR) spectroscopy, highlighting their principles and applications in radical scavenging assays and antioxidant characterization. Electrochemical techniques are rapid, sensitive, and cost-effective alternatives, focusing on voltammetry, amperometry, and biosensor development for direct measurement of electron transfer processes. The role of biosensors, employing bioreceptors like enzymes and DNA, for selective and sensitive antioxidant detection is also examined. Chromatographic methods, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), paired with selective detectors, are used to separate, identify, and quantify antioxidant compounds in complex matrices. This review highlights the principles, advancements, advantages, and limitations of these diverse methodologies in the accurate evaluation of antioxidant potential.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100558"},"PeriodicalIF":3.7,"publicationDate":"2025-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the role of α- and β-CDs in gold nanoparticle gel-based sensors for Fe³⁺ colorimetric detection 揭示α-和β-CDs在用于Fe +比色检测的金纳米颗粒凝胶传感器中的作用

IF 3.7

Talanta Open Pub Date : 2025-09-13 DOI: 10.1016/j.talo.2025.100557

Agustina Sus Andreani , Farrah Nurkhaliza , Muhammad Ridwan , Salsabila Freya Daniarti , Lilis Rosmaniar , Meiyanti Ratna Kumalasari

{"title":"Unveiling the role of α- and β-CDs in gold nanoparticle gel-based sensors for Fe³⁺ colorimetric detection","authors":"Agustina Sus Andreani , Farrah Nurkhaliza , Muhammad Ridwan , Salsabila Freya Daniarti , Lilis Rosmaniar , Meiyanti Ratna Kumalasari","doi":"10.1016/j.talo.2025.100557","DOIUrl":"10.1016/j.talo.2025.100557","url":null,"abstract":"<div><div>The detection of Fe<sup>3+</sup> in water samples is critical for mitigating potential harm to environmental and human health. This study presents a comparative investigation of α-cyclodextrin (α-CDs) and β-cyclodextrin (β-CDs) as stabilizing agents in the development of gold nanoparticle (AuNPs)-based gel sensors for Fe<sup>3+</sup> colorimetric sensing. The AuNPs were synthesized using ortho-hydroxybenzoic acid (o-HBA) as a reducing agent. The red-wine colloidal solutions of AuNPs-o-αCDs and AuNPs-o-βCDs were measured by a UV–Vis spectrophotometer, showing a surface plasmon resonance (SPR) band at 517 nm for both. Field emission scanning electron microscopy – energy dispersive X-ray (FESEM-EDX) analysis revealed well-dispersed AuNPs within the gel matrix. Particle size analysis (PSA) and zeta potential measurements further indicated a smaller particle size and superior colloidal stability in the AuNPs-o-βCDs. The analytical performance of both sensors was evaluated through precision, accuracy (%Recovery), and comparison with atomic absorption spectroscopy (AAS) via <em>t</em>-test assessments. Both sensors demonstrated acceptable precision and accuracy, showing no statistically significant difference from AAS results (<em>p</em> > 0.05). Notably, the gel-AuNPs-o-βCDs sensor exhibited enhanced sensitivity (0.20 mg/L) and faster detection, achieving stable readings within 15 min. In contrast, the gel-AuNPs-o-αCDs had a LoD of 0.57 mg/L and required 20 min for stable readout. Additionally, the gel-AuNPs-o-βCDs demonstrated a higher linear correlation with Fe<sup>3+</sup> concentration (R<sup>2</sup> = 0.9999) than the gel-AuNPs-o-αCDs (R<sup>2</sup> = 0.9994). These findings suggested that β-CDs provide superior stabilization and enhanced performance in the gel-based AuNPs sensors, establishing them as a highly promising candidate for rapid, reliable, and accurate Fe<sup>3+</sup> detection in tap water.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100557"},"PeriodicalIF":3.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stability and reproducibility study for the development of a potentiometric nitrate sensor for in-situ use 原位使用电位式硝酸盐传感器的稳定性和重复性研究

IF 3.7

Talanta Open Pub Date : 2025-09-11 DOI: 10.1016/j.talo.2025.100556

Michelle Brandão Silva de Assis , Kathrin Trommer , Alfred Kick , Michael Mertig

{"title":"Stability and reproducibility study for the development of a potentiometric nitrate sensor for in-situ use","authors":"Michelle Brandão Silva de Assis , Kathrin Trommer , Alfred Kick , Michael Mertig","doi":"10.1016/j.talo.2025.100556","DOIUrl":"10.1016/j.talo.2025.100556","url":null,"abstract":"<div><div>This study investigates the stability and reproducibility of a potentiometric nitrate sensor in all-solid-state configuration, consisting of a screen-printed graphite electrode, coated with electropolymerized polypyrrole as solid contact material, and covered by a TDMA-based ion-selective membrane. Special attention is given to the long-term stability, depending on storage and conditioning conditions as the most important factors for the applicability of the sensor. In particular, regression lines from calibration procedures, performed over a period of up to three months, were analyzed to evaluate the sensor performance. The sensor demonstrated superior stability, with minimal, nearly parallel shifts between regression lines. Notably, the sensor retained its ability to reproduce signals accurately even after one-month periods of dry storage, provided that the applied conditioning period was sufficiently long. The sensor was successfully applied for nitrate detection in drinking water samples, with a reproducibility of ± 3 mg/L, making it a promising candidate for real-time nitrate sensing applications. In all stages of the experiments, the sensor performance was compared with that of an all-solid-state sensor system, consisting of a gold electrode coated with poly(3-octylthiophene-2,5-diyl) and molybdenum disulfide nanocomposites as solid contact material.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100556"},"PeriodicalIF":3.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SensEDNA: An innovative optical CRISPR platform for rapid environmental DNA monitoring SensEDNA：一个创新的光学CRISPR平台，用于快速环境DNA监测

IF 3.7

Talanta Open Pub Date : 2025-09-10 DOI: 10.1016/j.talo.2025.100551

Molly Ann Williams , Ciprian Briciu-Burghina , Sean Power , Joyce O’Grady , Elvira de Eyto , Nigel Kent , Anne Parle-McDermott , Fiona Regan

{"title":"SensEDNA: An innovative optical CRISPR platform for rapid environmental DNA monitoring","authors":"Molly Ann Williams , Ciprian Briciu-Burghina , Sean Power , Joyce O’Grady , Elvira de Eyto , Nigel Kent , Anne Parle-McDermott , Fiona Regan","doi":"10.1016/j.talo.2025.100551","DOIUrl":"10.1016/j.talo.2025.100551","url":null,"abstract":"<div><div>Biodiversity loss is at an all-time high increasing the need for simple and rapid species monitoring systems. Management and conservation of fish species within aquatic environments requires knowledge of distribution, traditionally gained through visual detection and counting. These methods are expensive, time consuming and can lead to harm of the species of interest. Environmental DNA (eDNA) offers a solution to this through detection of DNA that may be shed into the environment by a given target species. Integrating eDNA based molecular assays to a biosensor device enables onsite sample testing for rapid species assessment.</div><div>Herein, a portable fluorometer with incubating capabilities (SensEDNA) was custom designed and built to conduct the simultaneous incubation and fluorescent detection steps required for isothermal eDNA detection. The system features three optical cells to enable triplicate analysis. Each optical cell is set to incubate at 37 °C and control within 0.5 °C. Fluorescence detection with excitation at 485 nm and emission at 535 nm was integrated and optimised to provide highest signal/noise ratio while minimising fluorescence bleaching. The system comprises a simple LED/high pass filter/photodiode configuration and is controlled by a Wixel board. This affordable and user-friendly optical detection platform, coupled with RPA-CRISPR-Cas, provides a crucial step towards on-site single species identification from eDNA.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100551"},"PeriodicalIF":3.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transforming a traditional ethnic beverage into highly emissive CDs as a sensitive nanosensor for the anti-malignant hyperthermia drug dantrolene sodium in pharmaceuticals and plasma. An economic and sustainable approach 将传统民族饮料转化为高发射cd，作为药物和血浆中抗恶性热疗药物丹曲林钠的敏感纳米传感器。经济和可持续的方法

IF 3.7

Talanta Open Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100549

Samar Salem, Rania El-Shaheny, Nora A. Abdallah

{"title":"Transforming a traditional ethnic beverage into highly emissive CDs as a sensitive nanosensor for the anti-malignant hyperthermia drug dantrolene sodium in pharmaceuticals and plasma. An economic and sustainable approach","authors":"Samar Salem, Rania El-Shaheny, Nora A. Abdallah","doi":"10.1016/j.talo.2025.100549","DOIUrl":"10.1016/j.talo.2025.100549","url":null,"abstract":"<div><div>This work provides a cost-effective, environmentally benign, rapidly prepared, highly fluorescent carbon dots (CDs) using renewable antioxidants-rich feedstock (<em>Tamarindus Indica</em> L.) as the precursor. A home-available and less energy-consuming microwave oven synthesizes CDs within 5 min. The created CDs have the highest fluorescence intensity at 402 nm after being excited at 328 nm, conferring a high luminescence quantum yield of 33.5 %. The prepared CDs provide ultra-sensitive and selective determination of dantrolene sodium (DAN), the only synthetic drug that can manage malignant hyperthermia up to now, and of extraordinary antioxidant properties. The determination is over a wide concentration range of (1.25–100.0 µM) with a limit of detection (LOD) and limit of quantification (LOQ) of 0.07 µM and 0.21 µM, respectively. DAN was estimated in two different pharmaceutical dosage forms, yielding percentage recoveries of 99.49 ± 1.50 and 99.74 ± 0.97. The proposed method was also successfully applied for the assay of DAN in spiked human plasma with a % recovery of 99.55 ± 3.13. Various greenness metrics were used to elucidate the greenness of the nanoprobe. The greenness of this work stems from the fabrication of CDs from natural, widespread, cheap, and edible fruit using a less energy and time-consuming, easily operated, and safe reactor. Moreover, avoiding harsh chemicals and solvents and using distilled water as the diluent renders the method greener. The above-mentioned facts about the proposed method prove it to be a validated analytical approach for different pharmaceutical and biological applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100549"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plasmonic fingerprinting: next-generation SERS architectures for sensitive heavy metal quantification 等离子体指纹识别：用于敏感重金属量化的下一代SERS架构

IF 3.7

Talanta Open Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100554

Muhammad Sarkawi , Rupam Sharma , Geetha Venkatesan , Tripti Ahuja , Soumyanti Panda , Jai Prakash , Hideki Kuramitz , K.S. Shalini devi

{"title":"Plasmonic fingerprinting: next-generation SERS architectures for sensitive heavy metal quantification","authors":"Muhammad Sarkawi , Rupam Sharma , Geetha Venkatesan , Tripti Ahuja , Soumyanti Panda , Jai Prakash , Hideki Kuramitz , K.S. Shalini devi","doi":"10.1016/j.talo.2025.100554","DOIUrl":"10.1016/j.talo.2025.100554","url":null,"abstract":"<div><div>The global heavy metal crisis demands sensing technologies that transcend traditional analytical limitations, positioning Surface-Enhanced Raman Scattering (SERS) as the transformative solution for real-world environmental and health monitoring challenges. This comprehensive review illuminates the sophisticated engineering principles underlying modern SERS architectures, where nanoscale precision meets molecular recognition to achieve detection limits that challenge the boundaries of single-molecule spectroscopy. Additionally, a systematic analysis of various SERS sensing strategies, including label-free approaches utilizing direct metal-nanoparticle interactions, molecular probe-based systems employing Raman-active chelating agents, and advanced functionalized nanoparticle platforms incorporating DNA aptamers, peptides, and polymeric recognition elements are discussed. Representative case studies include the successful detection of mercury in contaminated fish samples at sub-regulatory levels, lead quantification in urban drinking water systems, and arsenic monitoring in rice cultivation areas, showcasing the versatility and reliability of SERS-based approaches across diverse analytical challenges. The review concludes with a progressive perspective on the merging of SERS technology with microfluidics, Internet of Things architectures, and distributed sensing networks that will replace the current approach of testing samples periodically with continuous monitoring of heavy metals at specific locations.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100554"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fc-tagged PLA2R antigen enables high-performance chemiluminescent detection for non-invasive idiopathic membranous nephropathy diagnosis fc标记的PLA2R抗原可用于非侵袭性特发性膜性肾病的高效化学发光检测

IF 3.7

Talanta Open Pub Date : 2025-09-09 DOI: 10.1016/j.talo.2025.100555

Jiangjian Liu , Qiong Liang , Yanhang Zhuo, Sunhui Chen

{"title":"Fc-tagged PLA2R antigen enables high-performance chemiluminescent detection for non-invasive idiopathic membranous nephropathy diagnosis","authors":"Jiangjian Liu , Qiong Liang , Yanhang Zhuo, Sunhui Chen","doi":"10.1016/j.talo.2025.100555","DOIUrl":"10.1016/j.talo.2025.100555","url":null,"abstract":"<div><div>Idiopathic membranous nephropathy (IMN) is characterized by anti-phospholipase A2 receptor (PLA2R) antibodies, yet current detection methods lack sensitivity or are invasive. We developed a chemiluminescent assay to address these limitations. Three PLA2R antigens (Fc-tagged full, His-tagged, D3 core segment) were expressed in Expi293E cells and purified. Goat/mouse anti-human IgG antibodies were compared for detection efficiency. Kit stability was evaluated at 4 °C and 37 °C. Clinical validation included 75 biopsy-confirmed cases (50 IMN, 25 non-IMN), comparing the kit’s performance against a commercial ELISA. The PLA2R-Fc antigen demonstrated 100 % sensitivity and specificity, outperforming His-tagged (100 % sensitivity, 97 % specificity) and D3-truncated variants (60 % sensitivity). Coating in pH 6.0 MES buffer achieved the highest signal-to-noise ratio (45.08 vs. 25.76 in HEPES). Goat anti-human IgG enabled lower detection limits (22.5 U mL<sup>−1</sup> vs. 45 U mL<sup>−1</sup> for monoclonal antibody). The kit retained stable over 12 months at 4 °C. In clinical testing, the chemiluminescent assay showed superior concordance with histopathology, higher sensitivity, and specificity, particularly for low-titer samples. This chemiluminescent anti-PLA2R antibody assay combines high sensitivity, specificity, and stability, enabling non-invasive IMN diagnosis and therapeutic monitoring. Its technical optimizations address key limitations of existing methods, offering a reliable diagnostic tool for IMN.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100555"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0