Talanta Open最新文献

{"title":"Recent advances in analytical methods and instrumentation for natural antioxidants analysis","authors":"Suvarna Yenduri,&nbsp;Ramya P,&nbsp;Naga Prashant K","doi":"10.1016/j.talo.2025.100602","DOIUrl":"10.1016/j.talo.2025.100602","url":null,"abstract":"<div><div>Natural antioxidants, primarily produced from plants, have significant roles in avoiding oxidative stress related issues, enhancing shelf life of food, and improving human health. Because of the existence of wide range of antioxidants like flavonoids, phenolic acids, tannins, carotenoids, vitamins, it is difficult for characterization and evaluation of antioxidants. Conventional antioxidant assays are commonly employed till today; however they provide limited structural and biological insights. These constraints can be overcome by the application of advanced analytical techniques like chromatography, spectroscopy etc. which provide greater sensitivity and more accurate structural characterization. Recent research has highlighted the use of these approaches for qualitative and quantitative analysis, also for evaluating antioxidant activity in various matrices such as plant materials, food, nutraceuticals etc. The present study summarizes recent developments, comparison of various analytical methods and also highlighted the advantages and limitations of those methods. Emerging analytical techniques that combine green extraction methods for sample preparation, hyphenated analytical technique for analysis and chemometrics for data interpretation hold promise for reliable, rapid and sustainable natural antioxidant analysis.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100602"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145747007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green nanozyme, rapid sensing: A DES-synthesized Cu-BTC platform for rapid tetracycline detection in food","authors":"Xin Li ,&nbsp;Kaigang An ,&nbsp;Shuang Zhang ,&nbsp;Jing He ,&nbsp;Huifeng Liu ,&nbsp;Hongdeng Qiu ,&nbsp;Yiqun Wan ,&nbsp;Jia Chen","doi":"10.1016/j.talo.2025.100606","DOIUrl":"10.1016/j.talo.2025.100606","url":null,"abstract":"<div><div>Rapid detection of antibiotic residues in food is essential for public health. This study aims to develop a highly sensitive colorimetric sensing platform based on a novel nanozyme. To this end, we used a green, tunable, and safe choline chloride/urea-based deep eutectic solvent (DES) to synthesize a metal organic framework, Cu-BTC (BTC: 1,3,5-benzenetricarboxylate), with many highly dispersed copper active sites. As a result, the prepared Cu-BTC showed strong peroxidase-like activity. In the presence of hydrogen peroxide (H₂O₂), it efficiently catalyzed the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to the blue product TMBox. Importantly, we found that tetracycline (TC) specifically and significantly inhibits this color reaction. The inhibition occurred because TC can directly reduce the blue TMBox, suppressing color development. Based on this effect, we developed a new colorimetric method for sensitive TC analysis. The method had a wide linear range (0.025–25 μM) and a very low detection limit (0.0024 μM). Moreover, it was successfully applied to accurately detect TC in complex food matrices such as milk and honey. Thus, this work not only provides a green route to synthesize high-performance nanozymes, but also establishes a simple, fast, and sensitive colorimetric platform for on-site detection of antibiotic residues in food.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100606"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145921633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasmall CuFeMn nanozymes with multienzyme activity under neutral conditions: A smartphone-based colorimetric visualization of multiple biomarkers","authors":"Haiyi Xiong ,&nbsp;Liang Ye ,&nbsp;Na Ni ,&nbsp;Yan Zhang ,&nbsp;Jingkun Miao ,&nbsp;Jun Chen","doi":"10.1016/j.talo.2026.100620","DOIUrl":"10.1016/j.talo.2026.100620","url":null,"abstract":"<div><div>This study presents a novel nanozyme design with the development of ultrasmall trimetallic CuFeMn nanozymes (CF4M), which uniquely demonstrate dual peroxidase (POD)-like and oxidase (OXD)-like enzymatic activities under neutral pH conditions. By circumventing the limitation of conventional nanozymes that require acidic environments for optimal function, CF4M enables biosensing workflows without the need for buffer adjustments, significantly enhancing practical applicability. The CF4M utilizes its multivalent properties of Cu, Fe and Mn to ensure POD-like and OXD-like enzyme activity in neutral environments, addressing a major challenge in the field of biosensing. A versatile colorimetric platform utilizing CF4M achieves detection of multiple biomarkers, including glutathione (GSH), glucose (Glu), and glucose-6-phosphate dehydrogenase (G6PD) through cascading enzymatic reactions and TMB chromogenic responses. Integrated with smartphone-based imaging technology mediated by gold nanorods, the system facilitates user-friendly and visual quantification of multiple biomarkers. The CF4M-based biosensing platform establish a new paradigm for neutral-pH-compatible biosensors, offering broad utility in detect clinical biomarkers.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100620"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146073736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment and preliminary clinical evaluation of a high-sensitivity chemiluminescence immunoassay for serum IL-12p70 using fab' antibody fragments and acridinium ester","authors":"Yahui Song ,&nbsp;Yifei Wang ,&nbsp;Honghui Tang","doi":"10.1016/j.talo.2025.100605","DOIUrl":"10.1016/j.talo.2025.100605","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to establish a detection method for serum interleukin 12p70 (IL-12p70) using digested Fab' antibody fragments and acridine esterification chemiluminescence immunoquantitative analysis.</div></div><div><h3>Methods</h3><div>The double-antibody sandwich method was used with the complete IL-12p70 antibody labeled with acridine ester and an enzymatically digested antibody fragment of p40 labeled with biotin. A sandwich complex then formed with the analyte IL-12p70, in which biotin was captured by magnetic solid-phase particles coated with streptavidin, which glowed under alkaline hydrogen peroxide excitation. The luminescent signal was quantitatively analyzed. The stability of the labeled antibodies and the performance of the new quantitative method was evaluated. The clinical analysis results were compared with flow cytometry fluorescence to verify the clinical performance.</div></div><div><h3>Results</h3><div>Acridine ester sulfonamide and liposoluble biotin showed good stability as antibody labels. The limit of blank was 0.1 pg/mL and the limit of detection was 0.5 pg/mL, determined according to CLSI EP17-A2 with <em>n</em> = 60 replicates per batch with two independent batches. The linearity was 0.5–5000 pg/mL, and the precision was &lt; 5.5 %. The results of the new method and flow cytometry fluorescence were highly correlated (<em>P</em> &lt; 0.05). Interfering substances, including triglyceride, hemoglobin, bilirubin, rheumatoid factor, heterophilic human anti-mouse antibodies, and the structural analogs IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, and IL-23 had no significant effect on the results.</div></div><div><h3>Conclusion</h3><div>A method for detecting IL-12p70 in serum was established based on immunoquantitative analysis using Fab' antibody fragments, biotin and streptavidin amplification effects, and acridine ester chemiluminescence. The method exhibited high sensitivity, strong specificity, and a wide detection range, indicating its strong clinical application prospects.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100605"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145798482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AIE-active ferrocene conjugated imidazole fluorescent chromophores for “turn on/off” detection of hydrogen peroxide in mixed aqueous media and bio-imaging applications","authors":"Selvam Prabu,&nbsp;Nallasamy Palanisami","doi":"10.1016/j.talo.2026.100614","DOIUrl":"10.1016/j.talo.2026.100614","url":null,"abstract":"<div><div>We have synthesized ferrocene-appended Y-shaped methoxyphenyl-substituted imidazole derivatives 2-ferrocenyl-4,5-bis(4-methoxyphenyl)-1H-imidazole (<strong>1</strong>) and 2-ferrocenyl-4,5-bis((E)-4-methoxystyryl)-1H-imidazole (<strong>2</strong>), and characterized them using analytical and spectroscopic techniques (¹H, ¹³C NMR, FT-IR, and HR-Mass). Optical properties, including absorption, emission, quantum yield, and aggregation-induced emission (AIE), were demonstrated. The emission, ranging from weak to enhanced, was achieved through the AIE process in CH₃CN/H₂O mixtures by restricting intramolecular rotation (RIR). In the aggregated state, the quantum efficiency of chromophores <strong>1</strong> and <strong>2</strong> increases threefold and twofold, respectively, compared to the pure CH₃CN solution. Utilizing the AIE state, these chromophores were further employed to detect hydrogen peroxide (H₂O₂) in a mixed aqueous medium <em>via</em> a fluorescence <em>turn-on/off</em> approach. This was conducted with water fractions (<em>f_w</em>) of 80 % for chromophore <strong>1</strong> and 70 % for chromophore <strong>2</strong>, revealing a highly specific and sensitive fluorescence quenching response to H₂O₂ in the aqueous mixture. The response is rapid within a linear range of 0–110 µM and 0–80 µM, with detection thresholds of 24.7 nM for chromophore <strong>1</strong> and 30.9 nM for chromophore <strong>2</strong>, respectively. The limited detection capability is attributed to the absence of an alkene group in the chromophore, leading to low sensitivity due to the chromophore's high molecular rigidity, which impedes molecular motion. Moreover, chromophores <strong>1</strong> and <strong>2</strong> are non-cytotoxic (cell viability above 80 %), enabling them to detect intracellular H₂O₂ in human cervical carcinoma (HeLa) cells through fluorescence bio-imaging. Notably, the significant bright green fluorescence is quenched upon the addition of H₂O₂. Additionally, DFT/B3LYP calculations were used to explore the HOMO and LUMO energy levels and charge distribution of the optimized structure. The calculated HOMO and LUMO energies were compared with the experimentally obtained redox potential values.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100614"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthracene boronic acid methacrylate-based fluorescence activation assay for determination of glycated proteins in biological samples","authors":"Jaroslava Bezdekova ,&nbsp;Kristyna Pavelicova ,&nbsp;Dattatry Shivajirao Bhosale ,&nbsp;Silvie Kozakova ,&nbsp;Lenka Pavlikova ,&nbsp;Jihao Yu ,&nbsp;Jan Bartacek ,&nbsp;Jan Svoboda ,&nbsp;Milos Sedlak ,&nbsp;Andrew D. Miller ,&nbsp;Marketa Vaculovicova","doi":"10.1016/j.talo.2026.100617","DOIUrl":"10.1016/j.talo.2026.100617","url":null,"abstract":"<div><div>There is a current unmet medical need for the creation and development of point-of-care diagnostic tests/devices that can determine if a given individual is diabetic, pre-diabetic or normal. In pursuit of this objective, we report here on the successful evaluation of a bespoke anthracene boronic acid methacrylate (ABAM) in a rapid and simple fluorescence assay for the detection of glycated proteins known as disease biomarkers in human plasma samples. In so doing, we determine that increases in ABAM fluorescence intensity, as a function of glycated human serum albumin (gHSA) levels, in 50-fold diluted plasma samples, are sufficiently well resolved to suggest that ABAM could form the basis of a potential point-of-care diagnostic device to discriminate between blood plasma samples taken from individuals who are diabetic, pre-diabetic or normal. Indeed, our ABAM-based assay is shown to discriminate clearly in 50-fold diluted plasma samples between increases in gHSA plasma concentrations (from 5 mg L⁻¹ to 65 mg L⁻¹) above the normal base line, reaching final concentrations indicative of diabetes. In comparison to the nearest competitor, the fructosamine detection assay, our ABAM-based assay is twelve times faster, more than six times less expensive, requires ten times lower sample volumes, is both gHSA selective and specific, and is much less prone to interference from molecular interferents. Therefore, we would suggest that our ABAM-based assay has real future potential utility in diabetes disease management.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100617"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lemon zest-derived N,S-doped carbon quantum dots as a highly sensitive and sustainable fluorescent probe for cefoxitin sodium determination","authors":"Ahmed M. Abdel-Raoof ,&nbsp;Ebrahim A. El-Desouky ,&nbsp;Ahmed M. Abdelzaher ,&nbsp;Reem H. Obaydo","doi":"10.1016/j.talo.2025.100601","DOIUrl":"10.1016/j.talo.2025.100601","url":null,"abstract":"<div><div>Cefoxitin (CEF), classified as a semisynthetic beta-lactam second-generation cephalosporin antibiotic, can be used against many aerobic and anaerobic bacterial infections. Elevated aspartate aminotransferase levels and a higher prevalence of eosinophilia have been linked to higher CEF dosages, which alleviates the demand for ultra-sensitive determination and monitoring of its concentration level. Sulfur and nitrogen doped carbon quantum dots (N,S-CQDs) were synthesized by microwave techniques using lemon zest as a natural biomass source for green modified CQDs synthesis. N,S-CQDs used as a highly sensitive fluorescent probe, green and economic, for the determination of CEF owing to its excellent photoluminescence (PL) characters and exhibited higher quantum yield (QY) ∼37.65 % with high aqueous stability. The quenching mechanism is meticulously demonstrated to be a static quenching process through the utilization of fluorescence spectroscopy and Stern-Volmer analysis across various temperature conditions. This work developed a new method for rapid determination of CEF residues by static quenching mechanism with a detection limit (LOD) value of 0.023 µM within a linearity of 0.1–15.0 µM. Moreover, the proposed fluorescence probe has potential practical application for CEF determination in pharmaceutical dosage forms and biological fluids. Furthermore, four green evaluation tools, the Complex Modified Green Analytical Procedure Index (ComplexMoGAPI), the blue applicability grade index (BAGI), the carbon footprint reduction index (CaFRI), and a Modified Green Star Area (MoGSA), were evaluated to assess the proposed analytical method. The Evaluation and Performance of Practicality Index <em>(EPPI, 2025)</em> assessment demonstrated that the developed green N,S-CQDs-based fluorometric method for CEF determination is a more sustainable, efficient, and practical analytical approach compared to the reported derivatization method.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100601"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human errors in an analytical chemistry laboratory - Implementation of ordinal analysis of variation for risk assessment","authors":"Francesca R. Pennecchi ,&nbsp;Tamar Gadrich ,&nbsp;Ilya Kuselman ,&nbsp;D. Brynn Hibbert ,&nbsp;Angelique Botha ,&nbsp;Anastasia A. Semenova","doi":"10.1016/j.talo.2025.100603","DOIUrl":"10.1016/j.talo.2025.100603","url":null,"abstract":"<div><div>Decision-making risks caused by human errors in performing a chemical analysis are assessed using laboratory expert judgments (responses) on a specified ordinal scale. In the present paper, a new approach to assessment of risk is described based on implementation of the recently developed two-way ordinal analysis of variation – ORDANOVA. This approach calculates the number of expert responses related to the same category for each ordinal characteristic and then analyzes their relative frequencies as fractions of the total number of responses (of all categories) obtained for this characteristic. It does not violate the properties of ordinal data and allows for the correct interpretation of expert responses. Previously published expert responses on the risks in pH measurements of groundwater, in gas chromatography–mass spectrometry multi-residue pesticide analysis of fruits and vegetables, and in inductively coupled plasma–mass spectrometry analysis of geological samples, are analysed as examples. The datasets prepared for ORDANOVA calculations with the freely available software tool are provided in supplementary materials to the paper. The reduction of risk by different components of the laboratory quality system (QS) are estimated under several error scenarios. New multinomial scores characterizing risk reduction by the laboratory QS as a whole are proposed.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100603"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145749252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of activated carbon and β-cyclodextrin modified magnetic nanoparticles for extraction and determination of hippuric acid in urine samples","authors":"Xiaoyue Shan,&nbsp;Ji Shao,&nbsp;Ling Zhang,&nbsp;Yan Jin,&nbsp;Jiayi Qiu ,&nbsp;Siwei Tan,&nbsp;Haipeng Ye,&nbsp;Luoxian Yang","doi":"10.1016/j.talo.2026.100613","DOIUrl":"10.1016/j.talo.2026.100613","url":null,"abstract":"<div><div>In this study, a novel magnetic adsorbent (AC/β-CD/Fe₃O₄) was prepared for the magnetic solid phase extraction (MSPE) of hippuric acid (HA) from urine. The adsorbent combined the high surface area of activated carbon (AC), the host-guest recognition of β-cyclodextrin (β-CD), and the magnetic responsiveness of Fe₃O₄. Under the optimal conditions, the method demonstrated excellent performance for HA detection, with a linearity range of 0.0064-1.0 µg/mL, low limit of detection of 1.9 µg/L, and high precision (RSDs &lt; 5%). Recoveries from spiked urine matrices ranged from 95% to 99%, confirming the method's accuracy and practicality for monitoring HA in real biological matrices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100613"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-generation immuno-biosensors: Transforming foodborne pathogen detection","authors":"Sana Ahmed ,&nbsp;Park Tusan","doi":"10.1016/j.talo.2026.100612","DOIUrl":"10.1016/j.talo.2026.100612","url":null,"abstract":"<div><div>The persistent threat of foodborne pathogens, despite safety measures, underscores the urgent need for highly sensitive and rapid sensing technologies to prevent outbreaks and mitigate their devastating health and economic impacts. The evolution of sensing technologies, from enzyme-based biosensors to advanced immuno-based methods, highlights a continuous drive to improve sensitivity, speed, and feasibility in pathogen detection. Immuno-based biosensors have revolutionized pathogen detection by advancements in miniaturization, offering swift, sensitive, and cost-effective alternatives to traditional methods. Mainly, two categories of immuno-based biosensors have become largely demanding for POC; signal transduction-based, such as electrochemical, optical, and quartz crystal microbalance technologies, have significantly advanced pathogen detection by offering real-time, hyper-sensitive, and prompt monitoring capabilities, making them promising tools for field-deployable diagnostics. The second category, platform design-based immuno-based biosensors, such as lateral flow assays, microfluidic paper-based devices, and microchip-based devices, offer cost-effective, facile, and precise pathogen detection for use in food safety and outbreak prediction analysis. Nevertheless, numerous other biosensor types exist, but this review will focus on a few selective ones to enhance clarity and readability. The review shall summarize the state-of-the-art advancements in food pathogen sensing by immuno-based biosensors, their effectiveness, progress, and categories. Racing from qualification to quantification, the discussion will cover the challenges encountered and loopholes in the developed immuno-biosensing methodologies, including material-related issues (e.g., batch variability of nanomaterials, single-use paper substrates) and assay design (e.g., complex microfluidic architectures and multi-step protocols) that affect reproducibility, waste generation, and dependence on trained operators. It will reflect on a comparative study between the most recent emerging works based on good linear range, limit of detections (LODs), types of real samples utilized and short detection time for addressing their impact as POC devices. Unlike previous reviews that typically focus on isolated sensing mechanisms, this article provides a comparative analysis of both signal-transduction-based and platform-design-based immuno-biosensors, coupled with emphasis on recent advances relevant to point-of-care food pathogen detection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"13 ","pages":"Article 100612"},"PeriodicalIF":3.7,"publicationDate":"2026-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}