Talanta Open最新文献

{"title":"Development an automated and high -throughput analysis methods for detecting 64 anabolic androgenic steroids in urine using gas chromatography-mass spectrometry","authors":"Zhaobo Wu ,&nbsp;Aoxin Ma ,&nbsp;Lili Xing ,&nbsp;Xiaochen Huang ,&nbsp;Guojun Li ,&nbsp;Kaoqi Lian","doi":"10.1016/j.talo.2024.100362","DOIUrl":"10.1016/j.talo.2024.100362","url":null,"abstract":"<div><div>A gas chromatography tandem mass spectrometry (GC–MS/MS) assay was developed for the simultaneous detection of 64 anabolic androgenic steroids (AAS). Urine is extracted with tertbutyl methyl ether under alkaline conditions, purified with C18, and derived through silanization, Detection is carried out in dynamic multiple reaction monitoring (dMRM) mode, The overall recoveries ranged from 74.31 % to 119.66 %, The relative standard deviation is &lt;10 %, with a linear range of 1–200 ng/ml, The linear range of endogenous androsterone (A) and etiocholanolone (Etio) is 1.7–10,000 ng/ml, with a correlation coefficient 0.9991– 0.9999. The limit of detection (LOD) range of this experiment was 0.2 ng/ml-1.2 ng/ml, and limit of quantification (LOQ) was 0.7 ng/ml-4 ng/ml, The results of testing 100 actual samples show that this method is simple, effective and feasible, and can be used for the detection of AAS, meeting the requirements of the World Anti Doping Agency (WADA)</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100362"},"PeriodicalIF":4.1,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital identification and adulteration analysis of bupleurum chinense and bupleurum marginatum based on \"digital identity\" and UHPLC-QTOF-MSE","authors":"Xian rui Wang,&nbsp;Jia ting Zhang ,&nbsp;Wen guang Jing,&nbsp;Ming hua Li,&nbsp;Xiao han Guo,&nbsp;Xian long Cheng,&nbsp;Feng Wei","doi":"10.1016/j.talo.2024.100361","DOIUrl":"10.1016/j.talo.2024.100361","url":null,"abstract":"<div><h3>Background and objectives</h3><div>one of the legal sources of <em>Bupleuri Radix</em> (BR) is the <em>Bupleurum chinense</em> (BC), rather than the <em>Bupleurum marginatum</em> (BM). However, fake incidents of BM mixed with BC often occur in the market, which makes it more difficult to supervise the quality of BR and even endanger the life and health of patients. To strengthen the quality control and supervision of BP, we carried out digital identification and adulteration analysis of BC and BM based on the \"digital identity\" and UHPLC-QTOF-MS^E.</div></div><div><h3>Method</h3><div>firstly, the BC and BM were analyzed by UHPLC-QTOF-MS^E to obtain the quantized data characterization of chemical components. Secondly, the shared ions were extracted from different batches of BC and BM's control medicinal materials as their \"data representation of ions\", respectively. Then, the data matrices of unique ions of BC relative to BM and BM relative to BC were screened out, and the Top-N ions were outputted as the \"digital identities\" of BC and BM, sorted by ionic strength. Finally, the above \"digital identities\" of BC and BM were used as benchmarks for matching positive samples and commercial samples to feedback on matching credibility (MC).</div></div><div><h3>Results</h3><div>the results showed that based on the \"digital identities\" of BC and BM, the digital identification of BC, BM, and positive samples can be realized efficiently and accurately at the individual level of Chinese medicine, even if 3 % of BM in the mixed samples can still be identified efficiently and accurately. 10 batches of market samples were identified as adulterated samples. Furthermore, chemometric analysis has proven the reliability of BC and BM-based \"digital identity\" identification.</div></div><div><h3>Conclusion</h3><div>It proved that the identification and adulteration analysis of two herbs can be realized efficiently and quickly through the \"digital identities\" of BC and BM. It has important reference significance for developing non-targeted digital identification of herbal medicines at the individual level of Chinese medicine based on \"digital identity\", which was beneficial to the construction of digital Chinese medicine and digital quality control.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100361"},"PeriodicalIF":4.1,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted polymers-based biosensors for gynecological diagnostics and monitoring","authors":"Faezeh Ghorbanizamani ,&nbsp;Hichem Moulahoum ,&nbsp;Figen Zihnioglu ,&nbsp;Suna Timur","doi":"10.1016/j.talo.2024.100364","DOIUrl":"10.1016/j.talo.2024.100364","url":null,"abstract":"<div><div>Molecularly Imprinted Polymers (MIPs) offer promising advancements in gynecological diagnostics due to their high selectivity, stability, and cost-effectiveness. This review explores the application of MIP-based biosensors in detecting biomarkers for gynecological cancer, infections, and hormonal monitoring. Despite significant progress in MIP technology, its integration into clinical gynecology remains limited. The review provides a deep dive into the synthesis and characterization process of MIPs, current diagnostic methods, and the potential of emerging diagnostic approaches such as microfluidics and nanotechnology. Then, an overview of the various conditions, diseases, and potential biomarkers is explored. Emphasizing the importance of women's health, the review analyzes the latest research in MIP-based biosensing of gynecological conditions and calls for increased research and development to bridge the gap between laboratory innovation and clinical application. The goal is to enhance early detection, improve patient outcomes, and reduce healthcare costs. This advancement is essential for better disease management and personalized treatment in gynecology.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100364"},"PeriodicalIF":4.1,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in e-nose for potential applications in Covid-19 infection","authors":"Jay Rana,&nbsp;Sonal Desai","doi":"10.1016/j.talo.2024.100363","DOIUrl":"10.1016/j.talo.2024.100363","url":null,"abstract":"<div><div>E-nose devices are designed to detect odours and flavours. The versatility and sensitivity of e-nose technology make it a valuable tool in various fields including medical diagnosis, environmental monitoring, and security and safety. The utility of e-nose is extended to detect volatile organic compounds (VOCs) in breath and other bodily fluids that are indicative of COVID-19 infection. Several studies have shown promising results in terms of accuracy and sensitivity of e-nose devices for COVID-19 detection, and this technology has the potential to be used as a non-invasive and rapid screening tool for COVID-19 in high-risk environments such as hospitals and airports. However, further research and development are needed to optimize the sensitivity and specificity of e-nose devices for COVID-19 detection, and large-scale studies are needed to confirm the accuracy and effectiveness of these devices in real-world settings. This article covers the details about the purpose, working principle of e-nose along with its applicability for COVID-19 detection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100363"},"PeriodicalIF":4.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of marijuana using silver-phosphine ion complexation and a semi-quantitative 1 % decision-point assay","authors":"Alleigh N. Couch ,&nbsp;Jayleigh M. Lanza ,&nbsp;Christopher M. Zall ,&nbsp;J. Tyler Davidson","doi":"10.1016/j.talo.2024.100359","DOIUrl":"10.1016/j.talo.2024.100359","url":null,"abstract":"<div><div>Due to the strict federal regulations concerning ∆⁹-tetrahydrocannabinol (∆⁹-THC) content for the differentiation of hemp and marijuana outlined in the 2018 Farm Bill, the ability to quickly and reliably differentiate cannabis as marijuana or hemp is crucial within both the seized drug community and hemp industry. This study provides a novel direct mass spectrometry approach for the identification of marijuana using Ag-phosphine ion complexation and a semi-quantitative 1 % decision-point assay. The main constituents of hemp and marijuana, cannabidiol (CBD) and ∆⁹-THC, are isomeric and cannot be differentiated using soft ionization mass spectrometry techniques alone. However, the incorporation of [Ag(PPh₃)(OTf)]₂ enables the formation of unique MS/MS product ions at <em>m/z</em> 421/423, <em>m/z</em> 353/355, and <em>m/z</em> 231 for CBD due to differences in binding affinity, allowing CBD to be differentiated from ∆⁹-THC. Likewise, the isomeric cannabinoid precursors ∆⁹-tetrahydrocannabinolic acid (∆⁹-THCA) and cannabidiolic acid (CBDA) can be differentiated due to the formation of unique MS/MS product ions at <em>m/z</em> 465/467 and <em>m/z</em> 379/381, which are specific to CBDA. Eight additional cannabinoids were also characterized utilizing the proposed Ag-phosphine ion complexation approach. To reduce the potential for false positives, a more conservative 1 % decision-point assay was developed by fortifying 1 % weight-by-volume ∆⁹-THC-<em>d₉</em> into methanolic extracts of authentic cannabis plant material, followed by assessing if the total ∆⁹-THC content (composed of ∆⁹-THC and ∆⁹-THCA) was greater than or less than the intensity of the spiked internal standard. When the developed approach was applied to 20 methanolic extracts of authentic cannabis samples with known cannabinoid compositions, 90 % of the samples were correctly identified as marijuana or not marijuana based on the 1 % administrative threshold for the total ∆⁹-THC content. The two lone misidentifications were due to the presence of elevated ∆⁸-THC, which highlights the necessity to explore more selective ligands in the future. This study provides the first application of Ag-ligand ion complexation for the identification of marijuana based on a semi-quantitative 1 % decision-point assay, which shows great promise as an alternative method for the rapid identification of marijuana.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100359"},"PeriodicalIF":4.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel, simple and rapid assay to measure citrate level in bacterial culture for analysis of citrate consumption by bacteria","authors":"Rattiyaporn Kanlaya,&nbsp;Chonnicha Subkod,&nbsp;Visith Thongboonkerd","doi":"10.1016/j.talo.2024.100360","DOIUrl":"10.1016/j.talo.2024.100360","url":null,"abstract":"<div><div>Citrate is essential for Krebs cycle in eukaryotes and serves as a carbon source for some bacteria to survive/grow. Available methods for measuring citrate level rely mainly on enzymatic reactions and/or sophisticated instrumentations. This study aimed to develop a novel, simple and rapid assay to quantify citrate in bacterial culture for analysis of citrate consumption. The assay was initially tested with 0.1–25.6 mM citrate in deionized (dI) water or complete M9 medium without/with 0.25 M HCl. Wavelength scan revealed maximum absorption of citrate at λ210 nm with linear calibration curve (R² = 0.9997–0.9999) when HCl was added. Among negatively charged chemicals tested, only oxalate interfered with the assay. After 24-h inoculation of <em>Klebsiella pneumoniae</em> (the known citrate-utilizing bacterium) in (20 mM) citrate-containing complete M9 medium, the remaining citrate significantly decreased (by 22.20±7.13 % consumption). However, a mild decrease was also observed in the sample without bacterial inoculation, suggesting that some components of the complete medium interfered with the assay. It was clearly evidenced that M9 salt, CaCl₂ and/or MgSO₄ were responsible for such interference. Finally, citrate at low concentrations (0.1–6.4 mM) in M9 medium with CaCl₂ and/or MgSO₄ provided the linear calibration curve (R² = 0.9451–0.9986). At 5 mM, citrate consumption by <em>K. pneumoniae</em> after 24-h culture was 46.88±0.47 %. In summary, we have successfully developed a novel, simple and rapid method for measuring citrate level in bacterial culture. It will be very useful for measuring citrate consumption by typical and atypical citrate-utilizing bacteria for classification, mechanistic and pathophysiologic studies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100360"},"PeriodicalIF":4.1,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of 69 magnesium-free chlorophyll derivatives in decarboxylated extracts of medical Cannabis chemovars using ESI-LC-HRMS/MS","authors":"Anna Shapira ,&nbsp;Almog Uziel ,&nbsp;Shiri Procaccia ,&nbsp;Ohad Guberman ,&nbsp;Dan Y. Lewitus ,&nbsp;David Meiri","doi":"10.1016/j.talo.2024.100358","DOIUrl":"10.1016/j.talo.2024.100358","url":null,"abstract":"<div><div>The <em>Cannabis</em> plant contains many groups of bioactive metabolites with potential pharmacological properties. These properties have mainly been attributed to cannabinoids and terpenoids, with other groups receiving little attention. The usefulness of the abundant group of chlorophyll derivatives (CDs) was illustrated in various applications, but little is known regarding their presence and significance in <em>Cannabis</em>. We hypothesized that the heating accompanying the process extract preparation would result in a pool of CDs that have lost the central magnesium (Mg) ion. Herein, we introduce a liquid chromatography-tandem mass spectrometry approach for separating, identifying, and quantifying Mg-free CDs in <em>Cannabis</em> extracts after decarboxylation. We assessed 14 <em>Cannabis</em> cultivars representing the four types of chemovars and identified 69 distinct Mg-free CDs. These were separated into five families: Pheophorbide <em>a</em>, Pheophorbide <em>b</em>, Pheophytin <em>a</em>, Pheophytin <em>b,</em> and Purpurin 18. The structure of the Mg-free CDs was determined through their MS/MS fragmentation spectra as phytylated derivatives compared to dephytylated ones, and then further to pyroderivatives, alkylated and oxidized CDs. Substantial variation was found between the four different chemovar types and within different cultivars of the same chemovar, with the family of Pheophytin <em>a</em>, the most prevalent in all extracts. Type III chemovar high-cannabidiol plants contained significantly higher amounts of Mg-free CDs. These differences in abundance may result in variations in the therapeutic effects of plants that are considered similar according to their cannabinoid profiles.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100358"},"PeriodicalIF":4.1,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000729/pdfft?md5=e0a7d11ba7b4e38e58575db7e2b11f6b&pid=1-s2.0-S2666831924000729-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex detection of meningitis pathogens by a vertical flow paper microarray and signal enhancement suitable for low-resource settings: Proof of concept","authors":"Pedro Réu ,&nbsp;Giulia Gaudenzi ,&nbsp;Deborah Nanjebe ,&nbsp;Gustav Svedberg ,&nbsp;Dan Nyehangane ,&nbsp;Miren Urrutia Iturritza ,&nbsp;Phuthumani Mlotshwa ,&nbsp;Chris Hadjineophytou ,&nbsp;Jens Karlsson ,&nbsp;Jesper Gantelius ,&nbsp;Juliet Mwanga-Amumpaire ,&nbsp;Edmund Loh ,&nbsp;Helene Andersson Svahn ,&nbsp;Elias Kumbakumba ,&nbsp;Tobias Alfvén ,&nbsp;Yap Boum II ,&nbsp;Aman Russom","doi":"10.1016/j.talo.2024.100357","DOIUrl":"10.1016/j.talo.2024.100357","url":null,"abstract":"<div><h3>Objectives</h3><p>Meningitis is a medical emergency, and it is crucial to diagnose it accurately and promptly in order to manage patients effectively. It would, therefore, be essential to introduce and have fast, accurate, and user-friendly methods to determine the cause of these infections. This study aimed to demonstrate a potentially cost-effective new approach for detecting meningitis using a paper-based vertical flow microarray, which could be useful in settings with limited resources.</p></div><div><h3>Methods</h3><p>We describe a multiplex paper microarray for detecting <em>Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae</em>, and <em>Salmonella</em> spp. by the passive vertical flow of PCR-amplified clinical samples. A multibiotinylated amplicon was obtained as a product of PCR in the presence of both a biotinylated primer and biotin-11-dUTP. An enhancement step based on an enzyme-free gold enhancement protocol was also used to facilitate visual detection.</p></div><div><h3>Results</h3><p>This study showed that the vertical flow microarray (previously evaluated for one pathogen) can discriminately detect the amplification results down to the 10² copies of DNA limit for four meningitis pathogens in a multiplexed set-up. The study further demonstrated the ability of this device and setup to detect three of the four pathogens from clinical biosamples.</p></div><div><h3>Discussion</h3><p>This study demonstrated the capacity of a vertical flow microarray device to detect amplification products for four prevalent meningitis pathogens in a multiplex format. The vertical flow microarray demonstrated consistent visualization of the expected gene amplification results; however, indicating limitations in the pre- and amplification steps. This study highlights the potential of this multiplexing method for diagnosing meningitis and other syndromic diseases caused by various pathogens, especially in resource-limited areas.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100357"},"PeriodicalIF":4.1,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000717/pdfft?md5=95c976abd77a047beb9b9d2bfa54c603&pid=1-s2.0-S2666831924000717-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Affordable droplet-based flow analyzer with peristaltic micro-pumps for fluorescent ammonium sensing","authors":"Mingtao Sun ,&nbsp;Yiyu Jiang ,&nbsp;Wenshan Liang,&nbsp;Hui Zeng,&nbsp;Huiwen Chen,&nbsp;Min Zhang","doi":"10.1016/j.talo.2024.100356","DOIUrl":"10.1016/j.talo.2024.100356","url":null,"abstract":"<div><div>An affordable droplet-based flow analyzer incorporating peristaltic micro-pumps has been developed for fluorescent ammonium sensing. The cost-effective peristaltic micro-pumps, modified using 3D-printing techniques, feature a 3D-printed pump base integrated with a Hall sensor to monitor the rotation of the pump motor. This setup generates a pulse flow instead of a continuous flow, delivering specific volumes (typically between 3 to 4 μL) of solution with each rotation. By using separate pumps to deliver the aqueous and oil phases, these phases merge to form a droplet flowing stream. The relative standard deviation (RSD) values for droplet volumes range from 3.97 % to 5.99 % (<em>n</em>=50) across different pumps. The analyzer utilizes a reaction between ammonium, ortho-phthalaldehyde, and sulfite to produce a fluorescent derivative, allowing for sensitive detection of low ammonium concentrations. A custom light-emitting diode (LED)-based fluorescence detector has been fabricated using 3D printing, ensuring cost-effective production. The analyzer provides a limit of detection of 0.02 μM (3<em>σ</em>) and an RSD of 0.15 % (<em>n</em>=10, 1 μM ammonium). This analyzer offers several practical advantages, including reduced reagent consumption and the potential for further development in distributed on-site analysis. The use of 3D printing facilitates rapid prototyping and customization, making the system adaptable to various analytical applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100356"},"PeriodicalIF":4.1,"publicationDate":"2024-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000705/pdfft?md5=a87ae00c806aac22591c7aa4fe59acd8&pid=1-s2.0-S2666831924000705-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of spectrophotometric methods for estimation of arrhythmias drug-dronedarone hydrochloride in pure and dosage forms","authors":"Ragaa El Sheikh ,&nbsp;Ayman A. Gouda ,&nbsp;Ahmed A. Ghazy ,&nbsp;Nesma M. Jumaa ,&nbsp;Noha E.M. Elsaify ,&nbsp;Nehal S.A. Soliman ,&nbsp;Asmaa El Sayed ,&nbsp;Ahmed H. Moustafa ,&nbsp;Ahmad O. Babalghith ,&nbsp;Ahmed El Sayed ,&nbsp;Al-Sayed M．Abd El-Majeed","doi":"10.1016/j.talo.2024.100355","DOIUrl":"10.1016/j.talo.2024.100355","url":null,"abstract":"<div><p>Sensitive, simple, and accurate spectrophotometric methods have been developed for the assay of arrhythmias drug-dronedarone hydrochloride (DND) in bulk drug and pharmaceutical formulations. The proposed method is based on the oxidation reaction of DND with a known excess of cerium(IV) ammonium sulfate (Ce(IV)) as an oxidizing agent in acid medium, followed by the determination of the unreacted oxidant by adding a fixed amount of dye, e.g., amaranth (AM), methylene blue (MB), and indigocarmine (IC), followed by measuring the absorbance at 520, 664, and 610 nm, respectively. The effects of experimental conditions were studied and optimized. The beer's law was obeyed in the concentration ranges of 1.0–10, 1.0–15, and 1.0–8.0 μg mL^-1 using AM, MB, and IC dyes, respectively, with a correlation coefficient ≥ 0.9992. The calculated molar absorptivity values are 3.6527 × 10⁴, 3.1212 × 10⁴, and 4.229 × 10⁴ L mol^-1 cm^-1 using AM, MB, and IC dyes, respectively. The limits of detection and quantification were 0.30 and 1.0 µg mL^-1, respectively, for the three methods. Intra-day and inter-day accuracy and precision of the methods have been evaluated. No interference was observed from the additives. The proposed methods were successfully applied to the assay of DND in tablet preparations and the results were statistically compared with those of the reported method by applying Student's <em>t</em>-test and F-test. The reliability of the methods was further ascertained by performing recovery studies using the standard addition method.</p></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"10 ","pages":"Article 100355"},"PeriodicalIF":4.1,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666831924000699/pdfft?md5=f8ecd77d8a8d7d158b1cb91bf04e548c&pid=1-s2.0-S2666831924000699-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142238108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}