Talanta Open最新文献

{"title":"Unveiling the role of α- and β-CDs in gold nanoparticle gel-based sensors for Fe³⁺ colorimetric detection","authors":"Agustina Sus Andreani ,&nbsp;Farrah Nurkhaliza ,&nbsp;Muhammad Ridwan ,&nbsp;Salsabila Freya Daniarti ,&nbsp;Lilis Rosmaniar ,&nbsp;Meiyanti Ratna Kumalasari","doi":"10.1016/j.talo.2025.100557","DOIUrl":"10.1016/j.talo.2025.100557","url":null,"abstract":"<div><div>The detection of Fe³⁺ in water samples is critical for mitigating potential harm to environmental and human health. This study presents a comparative investigation of α-cyclodextrin (α-CDs) and β-cyclodextrin (β-CDs) as stabilizing agents in the development of gold nanoparticle (AuNPs)-based gel sensors for Fe³⁺ colorimetric sensing. The AuNPs were synthesized using ortho-hydroxybenzoic acid (o-HBA) as a reducing agent. The red-wine colloidal solutions of AuNPs-o-αCDs and AuNPs-o-βCDs were measured by a UV–Vis spectrophotometer, showing a surface plasmon resonance (SPR) band at 517 nm for both. Field emission scanning electron microscopy – energy dispersive X-ray (FESEM-EDX) analysis revealed well-dispersed AuNPs within the gel matrix. Particle size analysis (PSA) and zeta potential measurements further indicated a smaller particle size and superior colloidal stability in the AuNPs-o-βCDs. The analytical performance of both sensors was evaluated through precision, accuracy (%Recovery), and comparison with atomic absorption spectroscopy (AAS) via <em>t</em>-test assessments. Both sensors demonstrated acceptable precision and accuracy, showing no statistically significant difference from AAS results (<em>p</em> &gt; 0.05). Notably, the gel-AuNPs-o-βCDs sensor exhibited enhanced sensitivity (0.20 mg/L) and faster detection, achieving stable readings within 15 min. In contrast, the gel-AuNPs-o-αCDs had a LoD of 0.57 mg/L and required 20 min for stable readout. Additionally, the gel-AuNPs-o-βCDs demonstrated a higher linear correlation with Fe³⁺ concentration (R² = 0.9999) than the gel-AuNPs-o-αCDs (R² = 0.9994). These findings suggested that β-CDs provide superior stabilization and enhanced performance in the gel-based AuNPs sensors, establishing them as a highly promising candidate for rapid, reliable, and accurate Fe³⁺ detection in tap water.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100557"},"PeriodicalIF":3.7,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stability and reproducibility study for the development of a potentiometric nitrate sensor for in-situ use","authors":"Michelle Brandão Silva de Assis ,&nbsp;Kathrin Trommer ,&nbsp;Alfred Kick ,&nbsp;Michael Mertig","doi":"10.1016/j.talo.2025.100556","DOIUrl":"10.1016/j.talo.2025.100556","url":null,"abstract":"<div><div>This study investigates the stability and reproducibility of a potentiometric nitrate sensor in all-solid-state configuration, consisting of a screen-printed graphite electrode, coated with electropolymerized polypyrrole as solid contact material, and covered by a TDMA-based ion-selective membrane. Special attention is given to the long-term stability, depending on storage and conditioning conditions as the most important factors for the applicability of the sensor. In particular, regression lines from calibration procedures, performed over a period of up to three months, were analyzed to evaluate the sensor performance. The sensor demonstrated superior stability, with minimal, nearly parallel shifts between regression lines. Notably, the sensor retained its ability to reproduce signals accurately even after one-month periods of dry storage, provided that the applied conditioning period was sufficiently long. The sensor was successfully applied for nitrate detection in drinking water samples, with a reproducibility of ± 3 mg/L, making it a promising candidate for real-time nitrate sensing applications. In all stages of the experiments, the sensor performance was compared with that of an all-solid-state sensor system, consisting of a gold electrode coated with poly(3-octylthiophene-2,5-diyl) and molybdenum disulfide nanocomposites as solid contact material.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100556"},"PeriodicalIF":3.7,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SensEDNA: An innovative optical CRISPR platform for rapid environmental DNA monitoring","authors":"Molly Ann Williams ,&nbsp;Ciprian Briciu-Burghina ,&nbsp;Sean Power ,&nbsp;Joyce O’Grady ,&nbsp;Elvira de Eyto ,&nbsp;Nigel Kent ,&nbsp;Anne Parle-McDermott ,&nbsp;Fiona Regan","doi":"10.1016/j.talo.2025.100551","DOIUrl":"10.1016/j.talo.2025.100551","url":null,"abstract":"<div><div>Biodiversity loss is at an all-time high increasing the need for simple and rapid species monitoring systems. Management and conservation of fish species within aquatic environments requires knowledge of distribution, traditionally gained through visual detection and counting. These methods are expensive, time consuming and can lead to harm of the species of interest. Environmental DNA (eDNA) offers a solution to this through detection of DNA that may be shed into the environment by a given target species. Integrating eDNA based molecular assays to a biosensor device enables onsite sample testing for rapid species assessment.</div><div>Herein, a portable fluorometer with incubating capabilities (SensEDNA) was custom designed and built to conduct the simultaneous incubation and fluorescent detection steps required for isothermal eDNA detection. The system features three optical cells to enable triplicate analysis. Each optical cell is set to incubate at 37 °C and control within 0.5 °C. Fluorescence detection with excitation at 485 nm and emission at 535 nm was integrated and optimised to provide highest signal/noise ratio while minimising fluorescence bleaching. The system comprises a simple LED/high pass filter/photodiode configuration and is controlled by a Wixel board. This affordable and user-friendly optical detection platform, coupled with RPA-CRISPR-Cas, provides a crucial step towards on-site single species identification from eDNA.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100551"},"PeriodicalIF":3.7,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming a traditional ethnic beverage into highly emissive CDs as a sensitive nanosensor for the anti-malignant hyperthermia drug dantrolene sodium in pharmaceuticals and plasma. An economic and sustainable approach","authors":"Samar Salem,&nbsp;Rania El-Shaheny,&nbsp;Nora A. Abdallah","doi":"10.1016/j.talo.2025.100549","DOIUrl":"10.1016/j.talo.2025.100549","url":null,"abstract":"<div><div>This work provides a cost-effective, environmentally benign, rapidly prepared, highly fluorescent carbon dots (CDs) using renewable antioxidants-rich feedstock (<em>Tamarindus Indica</em> L.) as the precursor. A home-available and less energy-consuming microwave oven synthesizes CDs within 5 min. The created CDs have the highest fluorescence intensity at 402 nm after being excited at 328 nm, conferring a high luminescence quantum yield of 33.5 %. The prepared CDs provide ultra-sensitive and selective determination of dantrolene sodium (DAN), the only synthetic drug that can manage malignant hyperthermia up to now, and of extraordinary antioxidant properties. The determination is over a wide concentration range of (1.25–100.0 µM) with a limit of detection (LOD) and limit of quantification (LOQ) of 0.07 µM and 0.21 µM, respectively. DAN was estimated in two different pharmaceutical dosage forms, yielding percentage recoveries of 99.49 ± 1.50 and 99.74 ± 0.97. The proposed method was also successfully applied for the assay of DAN in spiked human plasma with a % recovery of 99.55 ± 3.13. Various greenness metrics were used to elucidate the greenness of the nanoprobe. The greenness of this work stems from the fabrication of CDs from natural, widespread, cheap, and edible fruit using a less energy and time-consuming, easily operated, and safe reactor. Moreover, avoiding harsh chemicals and solvents and using distilled water as the diluent renders the method greener. The above-mentioned facts about the proposed method prove it to be a validated analytical approach for different pharmaceutical and biological applications.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100549"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic fingerprinting: next-generation SERS architectures for sensitive heavy metal quantification","authors":"Muhammad Sarkawi ,&nbsp;Rupam Sharma ,&nbsp;Geetha Venkatesan ,&nbsp;Tripti Ahuja ,&nbsp;Soumyanti Panda ,&nbsp;Jai Prakash ,&nbsp;Hideki Kuramitz ,&nbsp;K.S. Shalini devi","doi":"10.1016/j.talo.2025.100554","DOIUrl":"10.1016/j.talo.2025.100554","url":null,"abstract":"<div><div>The global heavy metal crisis demands sensing technologies that transcend traditional analytical limitations, positioning Surface-Enhanced Raman Scattering (SERS) as the transformative solution for real-world environmental and health monitoring challenges. This comprehensive review illuminates the sophisticated engineering principles underlying modern SERS architectures, where nanoscale precision meets molecular recognition to achieve detection limits that challenge the boundaries of single-molecule spectroscopy. Additionally, a systematic analysis of various SERS sensing strategies, including label-free approaches utilizing direct metal-nanoparticle interactions, molecular probe-based systems employing Raman-active chelating agents, and advanced functionalized nanoparticle platforms incorporating DNA aptamers, peptides, and polymeric recognition elements are discussed. Representative case studies include the successful detection of mercury in contaminated fish samples at sub-regulatory levels, lead quantification in urban drinking water systems, and arsenic monitoring in rice cultivation areas, showcasing the versatility and reliability of SERS-based approaches across diverse analytical challenges. The review concludes with a progressive perspective on the merging of SERS technology with microfluidics, Internet of Things architectures, and distributed sensing networks that will replace the current approach of testing samples periodically with continuous monitoring of heavy metals at specific locations.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100554"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fc-tagged PLA2R antigen enables high-performance chemiluminescent detection for non-invasive idiopathic membranous nephropathy diagnosis","authors":"Jiangjian Liu ,&nbsp;Qiong Liang ,&nbsp;Yanhang Zhuo,&nbsp;Sunhui Chen","doi":"10.1016/j.talo.2025.100555","DOIUrl":"10.1016/j.talo.2025.100555","url":null,"abstract":"<div><div>Idiopathic membranous nephropathy (IMN) is characterized by anti-phospholipase A2 receptor (PLA2R) antibodies, yet current detection methods lack sensitivity or are invasive. We developed a chemiluminescent assay to address these limitations. Three PLA2R antigens (Fc-tagged full, His-tagged, D3 core segment) were expressed in Expi293E cells and purified. Goat/mouse anti-human IgG antibodies were compared for detection efficiency. Kit stability was evaluated at 4 °C and 37 °C. Clinical validation included 75 biopsy-confirmed cases (50 IMN, 25 non-IMN), comparing the kit’s performance against a commercial ELISA. The PLA2R-Fc antigen demonstrated 100 % sensitivity and specificity, outperforming His-tagged (100 % sensitivity, 97 % specificity) and D3-truncated variants (60 % sensitivity). Coating in pH 6.0 MES buffer achieved the highest signal-to-noise ratio (45.08 vs. 25.76 in HEPES). Goat anti-human IgG enabled lower detection limits (22.5 U mL⁻¹ vs. 45 U mL⁻¹ for monoclonal antibody). The kit retained stable over 12 months at 4 °C. In clinical testing, the chemiluminescent assay showed superior concordance with histopathology, higher sensitivity, and specificity, particularly for low-titer samples. This chemiluminescent anti-PLA2R antibody assay combines high sensitivity, specificity, and stability, enabling non-invasive IMN diagnosis and therapeutic monitoring. Its technical optimizations address key limitations of existing methods, offering a reliable diagnostic tool for IMN.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100555"},"PeriodicalIF":3.7,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of combination of protodioscin and shatavarin iv from medicinal extracts for alleviating menopausal symptoms by computational deep learning models","authors":"Rithika Naveencharan ,&nbsp;Ashwin Sivakumar ,&nbsp;Rishi Senthil Kumar ,&nbsp;Sheena Christabel Pravin ,&nbsp;Reena Monica P ,&nbsp;V. Kiruthika ,&nbsp;Abhijeet Morde ,&nbsp;Arun Balakrishnan ,&nbsp;Muralidhara Padigaru ,&nbsp;Ninad Puranik ,&nbsp;Ravindra Nayakwadi ,&nbsp;Tejas Namjoshi","doi":"10.1016/j.talo.2025.100552","DOIUrl":"10.1016/j.talo.2025.100552","url":null,"abstract":"<div><div>Shatavarin-IV and Protodioscin, saponins of Asparagus racemosus and Trigonella foenum-graecum respectively are phytoestrogens commonly used to alleviate post-menopausal symptoms such as hot flashes, sleeping disorder, and mood sways. While their individual benefits are well-documented, the synergistic effects of these compounds remain underexplored. This study aims to validate the combination of Shatavarin-IV and Protodioscin on molecular mediators of menopausal symptoms using advanced computational analyses. Deep learning model for drug additivity analysis was developed and trained on the DrugComb database using the Loewe’s additivity score as the target variable, with a focus on combinations tested on the MCF7 cell line and validated of the 786-O cell line. Molecular docking simulations were performed using PyRx, PyMOL, FPocket and ChimeraX to investigate the binding affinities of Protodioscin and Shatavarin-IV with key neuronal modulators of hot flashes, including Kisspeptin, Neurokinin B, TRPV1, c-FOS, and GnRH. IC50 values were derived from binding free energies. Dose-response simulations were evaluated across concentrations (0.01 µM to 100 µM) using a four-parameter logistic equation. Absorption, Distribution, Metabolism, and Elimination (ADME) profiling was determined to predict bioavailability and toxicity. The dose-response simulations confirmed significant therapeutic potential at relevant concentrations and additive interaction between both the compounds suggests enhanced therapeutic efficacy. ADME profiling indicated favorable bioavailability, non-toxicity, and efficient metabolism for both compounds and provides novel insights into their molecular mechanism of action in the body. The results of this research indicate that the combined additive effect of Protodioscin and Shatavarin-IV in alleviating menopausal symptoms exhibits favorable pharmacokinetic profile, and could potentially be promising candidates for future human clinical studies.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100552"},"PeriodicalIF":3.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thorium determination in seawater by inductively coupled plasma mass spectrometry: Method development and oceanographic applications","authors":"Yingjie Zou ,&nbsp;Peng Wang ,&nbsp;Lei Xu ,&nbsp;Hong Ren ,&nbsp;Yiyao Cao","doi":"10.1016/j.talo.2025.100553","DOIUrl":"10.1016/j.talo.2025.100553","url":null,"abstract":"<div><div>Thorium isotopes in the ocean (including radiogenic isotopes and terrigenous-derived ²³²Th) serve as key tracers for studying land-derived material transport. While high-sensitivity and high-resolution inductively coupled plasma mass spectrometry (ICP-MS) has become the mainstream technique for seawater thorium isotope analysis, its application faces three major challenges: complex pretreatment purification procedures, varying sample volume requirements, and significant instrument memory effects. This review systematically summarizes the technical difficulties and solutions in ICP-MS-based thorium isotope detection and examines its oceanographic applications in quantifying dust inputs and tracing water mass mixing, thereby providing critical data for assessing marine carbon pump efficiency.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100553"},"PeriodicalIF":3.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Renewable carbon from flax shives with silver nanoparticles biosynthesized using Eichhornia crassipes extract for green electrochemical detection of hydroxychloroquine","authors":"Francisco Contini Barreto ,&nbsp;Gloria Tersis Vieira dos Santos ,&nbsp;Maria Eduarda Barberis ,&nbsp;Naelle Kita Mounienguet ,&nbsp;Martin Kássio Leme da Silva ,&nbsp;Quan He ,&nbsp;Ivana Cesarino","doi":"10.1016/j.talo.2025.100548","DOIUrl":"10.1016/j.talo.2025.100548","url":null,"abstract":"<div><div>Hydroxychloroquine (HCQ) is an antimalarial drug that was repurposed during the COVID-19 pandemic. However, due to its limited clinical efficacy and notable side effects, its recommendation was later withdrawn. Despite this, HCQ sales increased by over 800 % in Brazil, and its presence has since been detected in aquatic environments, raising concerns regarding potential risks to both environmental and human health. In response, a novel green electrochemical sensor was developed for the determination of HCQ in water samples. The sensor is based on a glassy carbon electrode modified with hydrochar derived from flax shives and silver nanoparticles biosynthesized using <em>Eichhornia crassipes</em> (water hyacinth) extract as a reducing and stabilizing agent (GC/HC-AgNPs). The sensor was characterized by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and cyclic voltammetry (CV). Experimental parameters were optimized, and the device was successfully applied for the detection of HCQ using differential pulse voltammetry (DPV). The sensor exhibited a linear response in the range of 1.0–40.0 µmol L⁻¹, with a limit of detection (LOD) of 0.265 µmol L⁻¹ and a limit of quantification (LOQ) of 0.885 µmol L⁻¹. Excellent repeatability (RSD = 2.27 %) and reproducibility (RSD = 5.75 %) were achieved. Application in tap and lake water samples resulted in recovery values ranging from 89.5 % to 105.0 %, and comparative analysis with UV–vis spectroscopy confirmed the accuracy of the proposed method. The sensor further demonstrated good selectivity even in the presence of relevant interfering species. Green chemistry assessment tools confirmed the sustainable character of the developed method. Overall, the proposed sensor represents a promising, effective, and environmentally friendly platform for HCQ monitoring in water matrices.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100548"},"PeriodicalIF":3.7,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical detection of enrofloxacin and ciprofloxacin based on mesoporous La2O2CO3 nanostructures – an eco-friendly electrode material for sustainability","authors":"Arghya Chakravorty ,&nbsp;Sudip Das ,&nbsp;Sahil Luktuke ,&nbsp;Aarcha Appu Mini ,&nbsp;Vimala Raghavan","doi":"10.1016/j.talo.2025.100546","DOIUrl":"10.1016/j.talo.2025.100546","url":null,"abstract":"<div><div>Administration of antibiotics in the modern world has posed a serious threat to induce antimicrobial resistance (AMR), which concerns the Sustainable Development Goals (SDGs). Fluoroquinolone group antibiotics, namely enrofloxacin and ciprofloxacin, are predominantly responsible for undermining the One Health. In this context, to provide an eco-friendly and robust approach, a green electrochemical sensor has been fabricated with lanthanum oxycarbonate (La₂O₂CO₃) nanoparticles for the detection of enrofloxacin and ciprofloxacin in several complex matrices, related to food safety, environmental monitoring, and human health. The green synthesis methodology was adopted for the synthesis of the oxycarbonate material, while the phytochemicals present in the leaves of <em>Sida acuta</em> played the role of reducing and capping agents. To characterize the crystallinity and morphology of the synthesized material, several spectroscopic (XRD, BET, EDAX) and microscopic (FESEM, TEM) techniques were employed. The fabricated electrodes were characterized by atomic force microscopy (AFM), goniometry, and surface profilometry. The fabricated voltammetric sensor demonstrated an efficient linear range of 2.77 µM to 158.99 µM and 5.98 µM to 57.87 µM in the case of enrofloxacin and ciprofloxacin, respectively. In both cases, cyclic voltammetry (CV), differential pulse voltammetry (DPV), and square wave voltammetry (SWV) have been employed as electrochemical analytical techniques. The limit of detection (LOD) was achieved to be 4.07 µM, 2.91 µM, and 4.98 µM, while the limit of quantification (LOQ) was achieved to be 14.2 µM, 8.83 µM, and 15.1 µM in CV, DPV, and SWV, respectively, for the detection of enrofloxacin. On the other hand, the LOD was achieved to be 2.85 µM, 1.90 µM, and 1.65 µM, while the LOQ was found as 8.64 µM, 5.7 µM, and 5.01 µM in CV, DPV, and SWV, respectively, for the detection of ciprofloxacin.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100546"},"PeriodicalIF":3.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}