Talanta Open最新文献

{"title":"A fast, reproducible and sensitive human-plasma HPLC-fluorescence method to quantify Carvedilol and the error function","authors":"Antonio J. Braza,&nbsp;Montserrat Viñas-Bastart,&nbsp;Maria Sureda-Rosich,&nbsp;Cecilia F. Lastra,&nbsp;Eduardo L. Mariño,&nbsp;Pilar Modamio","doi":"10.1016/j.talo.2025.100544","DOIUrl":"10.1016/j.talo.2025.100544","url":null,"abstract":"<div><div>A robust and sensitive bioanalytical method was developed and fully validated using reverse-phase High-Performance Liquid Chromatography (HPLC) with fluorometric detection for the quantitative analysis of carvedilol in a complex matrix (human plasma). The method demonstrated satisfactory performance within the therapeutic concentration range (3.91–125 ng/mL), showing good linearity (coefficient of variation, CV: 12.05 %), accuracy (relative error, RE: 11.51 %), and precision—both intra-day (CV: 8.31 %) and inter-day (CV: 10.84 %)—as well as a recovery rate of 89.3 %. The lower limit of quantification (LLOQ) and the limit of detection (LOD) were 1.95 ng/mL and 0.98 ng/mL, respectively. Sample stability was confirmed for up to 24 h under autosampler conditions. The method yielded a short retention time of 3.15 minutes, enabling high-throughput analysis. Once validated, an analytical error function, expressed as a standard deviation (SD), was calculated to determine the most appropriate data-weighting approach across the calibration range. The best-fitting function was linear: SD (ng/mL) = 0.322 + 0.086C. An Analytical GREEnness Metric Approach (AGREE) of the method obtained a score of 0.67, indicating a moderately green profile. Due to its simplicity, cost-effectiveness, and rapid turnaround, the method is well suited for routine clinical use and may serve as a practical tool for monitoring medication adherence in healthcare settings, including hospitals.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100544"},"PeriodicalIF":3.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of BSA-modified silica for solid phase extraction of progesterone from river water samples","authors":"Mladen M. Đurđević ,&nbsp;Miloš P. Pešić ,&nbsp;Tatjana Ž. Verbić","doi":"10.1016/j.talo.2025.100545","DOIUrl":"10.1016/j.talo.2025.100545","url":null,"abstract":"<div><div>This study reports the development and application of bovine serum albumin (BSA)-modified silica gels as selective and durable sorbents for the solid-phase extraction (SPE) of progesterone from river water samples. One of the sorbents was used for SPE preconcentration followed by progesterone quantification in a sample collected from the Danube River. The standard addition method followed by high-performance liquid chromatography (HPLC-DAD) detection enabled the quantification of progesterone at a concentration of 143.0 ± 4.0 ng/L. At elevated progesterone concentrations (1.0 to 2.0 × 10^-7 M), recoveries approached 100 %, demonstrating the high efficiency of the developed extraction sorbents. The overall method recovery ranged from 91.4 to 101.0 %, confirming the method’s accuracy and robustness. Furthermore, under optimized SPE conditions, developed sorbents were highly selective towards progesterone compared to other reproductive hormones. The sorbents exhibited excellent long-term stability, with no significant change in binding efficiency observed over a four-year period (variation lower than 3 %), maintaining consistent recoveries of 94–96 %. Newly developed BSA-modified silica sorbents represent a promising tool for trace analysis of steroid hormones, as well as for their routine environmental monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100545"},"PeriodicalIF":3.7,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of patterned paper-based sensors for highly efficient formaldehyde detection","authors":"Taeuk Ji ,&nbsp;Gwangbeom Nam ,&nbsp;Shinwon Hwang,&nbsp;Minju Park,&nbsp;Jung Hwal Shin","doi":"10.1016/j.talo.2025.100541","DOIUrl":"10.1016/j.talo.2025.100541","url":null,"abstract":"<div><div>Paper-based sensors are widely used in formaldehyde (FA) sensing in various studies. The development of paper-based sensors with high accuracy and rapid response is important for FA detection. In this study, paper-based FA sensors (PFASs) were developed using a commercial inkjet printer, with filter paper as a substrate. The substrate was coated with a reactive solution containing two pH indicators: thymol blue (TB) and bromophenol blue (BPB), and hydroxylamine sulfate. To evaluate the performance of PFASs, the intensity of the color change caused by FA vapor was detected via colorimetry and optimized with respect to the TB:BPB ratio of the solution and the pattern formation during printing. The optimum TB:BPB ratio was 10:1. The response time decreased as the pattern line width decreased and the pore size increased, and check patterns showed a faster response than those of regularly spaced lines. The sensor was tested in an environment with a relative humidity of 50 ± 5 % at room temperature to ensure consistent experimental conditions, minimizing any potential interference from external factors. The color change of the sensor was visibly noticeable for FA concentrations above 1 ppm. Thus, the proposed technique shows great promise for rapid, high-precision FA detection.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100541"},"PeriodicalIF":3.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green rapid HPLC method for testing retinol and tocopherol in ophthalmic gels","authors":"Xiao-Chen Huang ,&nbsp;Ahmed Sobhy Darwish ,&nbsp;Wageh Sobhy Darwish ,&nbsp;Ri-Meng Chen ,&nbsp;Jin-Kui Ma","doi":"10.1016/j.talo.2025.100538","DOIUrl":"10.1016/j.talo.2025.100538","url":null,"abstract":"<div><div>In addition to providing immediate comfort for dry and irritated eyes, vitamin A (retinol) and E (tocopherol) drops strengthen the eyes' defenses. Optic gels, which are compatible with contact lenses, use vitamins A and E to improve vision, soothe and hydrate dry and sensitive eyes, and provide immediate comfort. It is now feasible to determine the amounts of vitamin A and vitamin E in ocular gels thanks to the development and validation of a thorough, rapid, simple, and eco-friendly stability-indicating LC approach. In compliance with ICH guidelines, the two medications underwent a series of assessments. Vitamin A palmitate, vitamin E acetate, and related degradants were successfully resolved via isocratic separation. Utilizing the octadecylsilyl phase at ambient temperature (25° <em>C</em> ± 5°C), both drugs were quantified and qualified. Constant ratios of filtered water (neutral purified water at pH range 5 to 7) to ethanol comprise the isocratic mobile phase, which has a flow rate of 2.0 mL/min. During the 290 nm detection, the concentrations of vitamin A palmitate and vitamin E acetate varied from 5 to 200 IU/mL and 25 to 1000 μg/mL, respectively. The LC technique's ecological benefits are demonstrated by its overall AES grade of 79, AGREE grade of 0.64, MoGAPI grade of 76, BAGI grade of 80, and final whiteness of 95.8. According to research, the suggested method is accurate, reliable, long-lasting, eco-friendly, and distinctive.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100538"},"PeriodicalIF":3.7,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145007537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanogram-level green spectrofluorimetric method for simultaneous quantification of tadalafil and silodosin in pharmaceuticals, environmental matrices and content uniformity testing","authors":"Aya Saad Radwan ,&nbsp;Mohamed Hefnawy ,&nbsp;Talha Bin Emran ,&nbsp;Mohamed M. Salim","doi":"10.1016/j.talo.2025.100534","DOIUrl":"10.1016/j.talo.2025.100534","url":null,"abstract":"<div><div>A novel, eco-friendly spectrofluorimetric method has been developed and validated for the first time for simultaneous determination of tadalafil (TDF) and silodosin (SLD) in pharmaceutical dosage forms and environmental water samples without the need for prior separation. This innovative approach exploits the emission fluorescence of both drugs, employing excitation at 270 nm and emission detection at 328 nm for TDF and 454 nm for SLD. The method demonstrated excellent linearity across the concentration ranges of 50.0–1200.0 ng mL^-1 for TDF and 1.0–300.0 ng mL^-1 for SLD (r &gt; 0.9999), with remarkable limits of detection of 10.94 ng mL^-1 and 0.28 ng mL^-1, respectively. The high sensitivity and precision (RSD &lt; 0.70 %), with recovery rates ranging from 99.50 % to 101.00 % in pharmaceuticals and 97.31 % to 102.83 % in environmental water emphasize the method’s robustness and practical applicability for therapeutic drug monitoring and environmental surveillance. Application to content uniformity testing of tablet formulations, in accordance with British Pharmacopoeia guidelines, further demonstrates the method’s versatility. Greenness assessments using the AGREE and complex moGAPI metrics, alongside operational efficiency evaluation through the Click Analytical Chemistry Index (CACI), confirm the method’s sustainability and minimal environmental impact. Fully compliant with ICH Q2(R1) guidelines, this validated protocol offers a pioneering, rapid, and sustainable alternative for routine quality control and environmental monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100534"},"PeriodicalIF":3.7,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Material matters: evaluating polymer 96-well plates to reduce DAMGO loss in LC-MS/MS-based in vitro μ-opioid structure affinity assay","authors":"Gaia Alluisetti ,&nbsp;Emma Van Impe ,&nbsp;Wolfgang Weinmann ,&nbsp;Katharina Elisabeth Grafinger","doi":"10.1016/j.talo.2025.100543","DOIUrl":"10.1016/j.talo.2025.100543","url":null,"abstract":"<div><div>The peptide DAMGO has been used for decades to determine binding affinity at the μ-opioid receptor in numerous types of receptor binding assays utilizing radiolabeled DAMGO, and more recently, liquid chromatography tandem mass spectrometry (LC-MS/MS)-based procedures with unlabeled DAMGO. While previously there have been numerous reports of non-specific adsorption (NSA) at plastic surfaces of various peptides, this study reports for the first time the NSA of DAMGO at polymer 96-well plates used in receptor binding affinity assays. In the present study, seven different types of polypropylene and one modified polystyrene 96-well plates were assessed for DAMGO NSA. The number of solution transfers, two different incubation temperatures and times were evaluated. NSA and DAMGO signal loss were observed for six out of eight tested plates. Best results were obtained with Thermo Scientific (Cat. No 60180-P133) 96-well deep-well plates, for which no significant signal deviation could be observed compared to the blanks even after six sequential transfers. Coated microtiter plates from Greiner Bio-One (Cat. No 655901) showed significant signal enhancement compared to the blanks, which may also cause issues when performing quantitative analysis of results. This study highlights the necessity of testing laboratory equipment for NSA during assay development, since peptide or analyte adsorption may not be immediately apparent, leading to the issue going unnoticed.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100543"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in photoelectrochemical sensing of tetracyclines","authors":"Yali Cui ,&nbsp;Yanyuan Zhang ,&nbsp;Lin Wang ,&nbsp;Yuanqiang Hao","doi":"10.1016/j.talo.2025.100542","DOIUrl":"10.1016/j.talo.2025.100542","url":null,"abstract":"<div><div>Tetracyclines (TCs) are widely used antibiotics whose residues pose environmental and health risks, necessitating sensitive and selective detection. Photoelectrochemical (PEC) sensors have emerged as promising analytical tools due to their high sensitivity, low background noise, and cost-effective instrumentation. This review systematically summarizes recent advances in PEC sensing of TCs. We begin by categorizing the photoactive materials employed in PEC sensing and examining their structural design strategies, heterojunction engineering, and photoelectric properties. Subsequently, we elaborate on the fundamental signal transduction mechanisms, including the intrinsic redox activity of TCs, steric hindrance-induced current suppression, and coordination-mediated modulation of interfacial charge transfer. Furthermore, we highlight representative PEC sensing systems reported for TCs detection, classifying them based on recognition strategies such as aptamer binding, molecular imprinting, electrostatic adsorption, and site-specific coordination—each linked to distinct signal generation modes. Finally, current limitations and promising strategies for further performance enhancement of PEC sensors are discussed. This review provides a comprehensive perspective on PEC strategies for TCs detection and offers guidance for future innovations in environmental and food safety monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100542"},"PeriodicalIF":3.7,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality-by-design optimized HPLC approach for the therapeutic drug monitoring of isosorbide dinitrate and sildenafil in human plasma samples","authors":"Mohamed A. Momtaz ,&nbsp;Hanaa S. El-Desoky ,&nbsp;Ahmed Rehab ,&nbsp;Fathalla Belal","doi":"10.1016/j.talo.2025.100540","DOIUrl":"10.1016/j.talo.2025.100540","url":null,"abstract":"<div><div>Isosorbide dinitrate (ISDN) and sildenafil citrate (SIL) are reported to have a potential drug-drug interaction when co-administered clinically. <strong>ISDN</strong> is indicated as first-line therapy for the long-term management of chronic stable angina. <strong>Sildenafil</strong> is the first oral treatment for erectile dysfunction. It is contra-indicated in patients who may require organic nitrates, such as ISDN, because this combination may cause a sudden drop in blood pressure by inhibiting the phosphodiesterase type 5 enzyme. Additionally, sildenafil is effective in treating cardiovascular disorders secondary to endothelial dysfunctions. Therefore, a sensitive, simple, and selective HPLC method is urgently needed for their simultaneous determination in plasma samples for monitoring their therapeutic blood concentration levels in emergency cases. The current study introduces the first HPLC approach for their simultaneous estimation in less than 10 minutes. The optimal separation was achieved using Nova-Pack® C18, 4 µm column at room temperature, with a mobile phase consisting of acetonitrile and acetate buffer (5 mM; pH 5, 39:61 % v/v), delivered at a flow rate of 1.1 mL/min. The injection volume was set at 50 µL, and detection was accomplished at 214 nm. The method demonstrated excellent linearity for ISDN(0.01–10.0µg/mL) and SIL (0.025–10.0µg/mL), with LOQ of 0.01 µg/mL and 0.020 µg/mL, respectively. The proposed method was successfully applied to analyze spiked human samples with recovery rates (104.9 and 105.55%) for ISDN and SIL respectively, confirming its suitability for bio-analytical use. Utilizing the quality-by- design (QbD) approach adopting the two-level full factorial design allowed the optimization of the experimental conditions of the proposed method. The method’s greenness was evaluated using Analytical Eco-Scale, Green Analytical Procedure Index (GAPI), and Analytical Greenness (AGREE). Validation of the proposed method was performed in accordance with ICH and US FDA guidelines.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100540"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on biosensor approaches for the detection of hazardous elements in water","authors":"Nidhi Chauhan ,&nbsp;Nishtha Sah ,&nbsp;Kirti Saxena ,&nbsp;Utkarsh Jain","doi":"10.1016/j.talo.2025.100536","DOIUrl":"10.1016/j.talo.2025.100536","url":null,"abstract":"<div><div>Water is the basis of life on Earth, but due to increased pollution and habitat degradation, water scarcity is increasing day by day. Therefore, it is necessary to detect water pollutants to save water and aquatic diversity. Monitoring of toxic elements present in wastewater effluents is an important factor for further treatment to make it pollutant-free. While various methods have been developed for monitoring these toxic elements, biosensors can overcome the limitations of traditional methods. Biosensors are highly sensitive, selective, low in cost, and simple to use. In this review, we have provided an overview of reported biosensors for the detection of various types of toxic elements that cause water toxicity. A summary of these toxic elements has also been discussed herein. This review aims to provide an overview of advances in biosensing technology used for water monitoring.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100536"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An analytical method to quantify tetrabromobisphenol A (TBBPA) and its conjugated metabolites in rat serum or other biological samples","authors":"Mette Stub,&nbsp;Mikael Pedersen,&nbsp;Louise Ramhøj,&nbsp;Kit Granby,&nbsp;Terje Svingen,&nbsp;Agnieszka Anna Niklas","doi":"10.1016/j.talo.2025.100537","DOIUrl":"10.1016/j.talo.2025.100537","url":null,"abstract":"<div><div>Brominated flame retardants (BFRs) are widely used to enhance fire resistance in consumer and commercial products, but often leach into the environment due to their surface application. Tetrabromobisphenol A (TBBPA), the most applied BFR, has been detected in human serum, urine, and breast milk. TBBPA is readily absorbed from the gastrointestinal tract, undergoing first-pass hepatic metabolism to form the two major metabolites TBBPA-sulfate and TBBPA-glucuronide. Despite evidence linking TBBPA exposure to developmental toxicity, neurobehavioral effects, uterine cancers, and endocrine disrupting potential via thyroid hormone interference, exposure assessments remain challenging. Current methods rely heavily on direct quantification of TBBPA and its metabolites using liquid chromatography-mass spectrometry (LC-MS), but a lack of commercially available TBBPA metabolites at analytical standards is a limiting factor. This study introduces a method to rapidly quantify TBBPA, and after enzymatic deconjugation, its metabolites using LC-Orbitrap. Using this method, TBBPA-glucuronide was the only metabolite detected in rat serum following oral exposure. Native TBBPA was obtained using enzymatic deconjugation of serum. The protocol simplifies exposure evaluation by offering a practical, and rapid, approach for quantifying TBBPA in biological samples.</div></div>","PeriodicalId":436,"journal":{"name":"Talanta Open","volume":"12 ","pages":"Article 100537"},"PeriodicalIF":3.7,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144911960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}