Nano-MIP based SPR sensor for tetracycline analysis in milk sample

IF 4.1 Q1 CHEMISTRY, ANALYTICAL
Monireh Bakhshpour-Yücel, Melike Küçük, Elif Tümay Özer, Bilgen Osman
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引用次数: 0

Abstract

Recent advancements in sensor technology have enabled the detection of antibiotics in food, ensuring human safety. In this study, we developed a surface plasmon resonance (SPR) sensor based on molecularly imprinted nanoparticles (MINps) for the real-time, sensitive, and in-situ detection of tetracycline (Tc). Firstly, Tc-imprinted nanoparticles (Tc-MINps) were synthesized using microemulsion polymerization. Then, the Tc-MINps were coated onto a bare gold SPR chip to develop the Tc-MINps SPR sensor. The sensor's performance was evaluated by detecting Tc in aqueous solutions. The results demonstrated a highly selective binding of Tc to the nanocavities on the surface of the Tc-MINps SPR sensor. The relationship between Tc molecules and the SPR sensor was analyzed at 0.5–20 mg/L Tc concentrations (pH 5.0). The Langmuir isotherm model was identified as the most appropriate binding model, indicating monolayer adsorption. The selectivity of the Tc-MINps SPR sensor was investigated using oxytetracycline, ciprofloxacin, and amoxicillin due to their structural similarity. The selectivity coefficients were determined as 5.54 for oxytetracycline, 23.66 for ciprofloxacin, and 28.39 for amoxicillin. Additionally, the limit of detection (LOD) for the Tc-MINps SPR sensor for Tc analysis in milk was found to be 0.45 mg/L, while the LOD for the HPLC method was 0.55 mg/L. The developed SPR sensor is suitable for Tc detection in milk due to its advantages, such as real-time monitoring, low cost, high selectivity, and reusability.

Abstract Image

基于纳米mip的SPR传感器用于牛奶样品中四环素的分析
最近传感器技术的进步使检测食品中的抗生素成为可能,确保了人类的安全。在这项研究中,我们开发了一种基于分子印迹纳米颗粒(MINps)的表面等离子体共振(SPR)传感器,用于实时、灵敏和原位检测四环素(Tc)。首先,采用微乳液聚合法制备了Tc-MINps印迹纳米粒子。然后,将Tc-MINps涂覆在裸金SPR芯片上,以开发Tc-MINps SPR传感器。通过对水溶液中Tc的检测来评价传感器的性能。结果表明Tc与Tc- minps SPR传感器表面的纳米空腔具有高度选择性的结合。在0.5 ~ 20 mg/L Tc浓度(pH 5.0)下,分析Tc分子与SPR传感器的关系。Langmuir等温线模型是最合适的结合模型,表现为单层吸附。由于结构相似,采用土霉素、环丙沙星和阿莫西林考察了Tc-MINps SPR传感器的选择性。土霉素、环丙沙星和阿莫西林的选择性系数分别为5.54、23.66和28.39。此外,Tc- minps SPR传感器用于牛奶中Tc分析的检出限(LOD)为0.45 mg/L, HPLC法的检出限为0.55 mg/L。SPR传感器具有实时监测、低成本、高选择性和可重复使用等优点,适用于牛奶中Tc的检测。
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来源期刊
Talanta Open
Talanta Open Chemistry-Analytical Chemistry
CiteScore
5.20
自引率
0.00%
发文量
86
审稿时长
49 days
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