Sensors and Actuators Reports最新文献

{"title":"An AIE active imidazole conjugated α-cyanostilbene based sensor for the selective and sensitive detection of picric acid in an aqueous medium","authors":"Selin Manoj Kumar,&nbsp;Sathiyanarayanan Kulathu Iyer","doi":"10.1016/j.snr.2023.100177","DOIUrl":"https://doi.org/10.1016/j.snr.2023.100177","url":null,"abstract":"<div><p>Due to increasing pollution threats, terrorism-sensitive rapid sensing of aromatic nitro explosives (picric acid) has gained predominant significance in environmental safety. Herein, imidazole-derived monofunctional fluorescent sensor 2-(4-aminophenyl)-3-(3-(4,5-diphenyl-1<em>H</em>-imidazol-2-yl)-2-hydroxyphenyl) acrylonitrile (<strong>ADHA)</strong> was successfully developed for the selective recognition of explosive picric acid. The D-π-A configuration of <strong>ADHA</strong> facilitates extraordinary photophysical properties with remarkable aggregation-induced emission (AIE). The detection process is induced by the photon-induced electron transfer (PET) and resonance energy transfer (RET) and results in the generation of <strong>ADHA</strong>+PA complex. The structural relationship and the photophysical properties of <strong>ADHA</strong> were extensively studied by DFT (Density Functional Theory) methods and spectroscopic analysis. It has been calculated that the detection limit of the formed complex is 6.26 nM. In addition, ¹H NMR titrations DFT calculations, and HRMS analysis were performed to understand the detection mechanism better. Test strip-aided detection and invisible ink applications confirmed that <strong>ADHA</strong> is a versatile sensor for sensitively detecting picric acid without sophisticated instruments. In addition, <strong>ADHA</strong> was implemented to detect PA in real water samples with remarkable recovery.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100177"},"PeriodicalIF":5.9,"publicationDate":"2023-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053923000401/pdfft?md5=dc9ee271531b158ab41117437af8ca54&pid=1-s2.0-S2666053923000401-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91684800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroanalytical Overview: The Electroanalytical Detection of Oxalate","authors":"Robert D. Crapnell,&nbsp;Prashanth S. Adarakatti,&nbsp;Craig E. Banks","doi":"10.1016/j.snr.2023.100176","DOIUrl":"https://doi.org/10.1016/j.snr.2023.100176","url":null,"abstract":"<div><p>The sensing of oxalate within urine has been recognised as one of the most important determinations in the investigation of patients with hyperoxaluria. However, current approaches have reported expensive, time consuming, occasionally poor selectivity and are subject to large inaccuracies if great care is not exercised in the handling and measurement of samples. One approach is the use of electroanalytical sensors, which present rapid but highly selective and sensitive outputs, are economical and miniature providing portable sensing platforms to support on-site analysis. In this minireview, recent advances in the electroanalytical sensing of oxalate are presented, overviewing recent electrode configurations and real sample analysis; comparisons to other analytical methods are presented. Finally, the conclusions and future perspective of this field are described in brief.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100176"},"PeriodicalIF":5.9,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053923000395/pdfft?md5=4185ced973ed06dff7765fa2fb54e5f3&pid=1-s2.0-S2666053923000395-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92098417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of MXene-based electrochemical biosensors for biomolecule and pathogen detection","authors":"Aibobek Seitak ,&nbsp;Shaohong Luo ,&nbsp;Ning Cai ,&nbsp;Kin Liao ,&nbsp;Anna-Maria Pappa ,&nbsp;Sungmun Lee ,&nbsp;Vincent Chan","doi":"10.1016/j.snr.2023.100175","DOIUrl":"https://doi.org/10.1016/j.snr.2023.100175","url":null,"abstract":"<div><p>MXenes, is an attractive new class of two-dimensional (2D) materials, discovered in 2011. Since then, owing to their unique combination of properties, such as high specific area, high electrical conductivity, tunable hydrophilicity, tunable chemical composition, and potential cytocompatibility, MXenes have made a deep impact on various fields ranging from electronics to energy and more recently to biotechnology. A typical example for the latter, is their use as electroactive biointerfaces in a number of biosensor setups, exhibiting remarkable analytical performance. In particular, MXene-based nanocomposites can serve as bioreceptors, electrochemical transducers or amplification probes towards translating molecular recognition of biological targets into detectable signals, leading to ultrasensitive biosensors for probing biomarkers, or pathogens. This concise review highlights the recent advances of MXene-based electrochemical biosensors for highly selective and sensitive detection of nucleic acids, proteins and pathogens pertaining to biomarker identification and clinical diagnostics. In particular, the effects of synthetic routes, surface chemistry, nanocomposite design, and fabrication methods of MXenes on the resulting relationship between biointerfacial structure, electrochemical properties and device performance is discussed, providing unique perspectives and design criteria for the next wave of biosensors.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100175"},"PeriodicalIF":5.9,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053923000383/pdfft?md5=1484e760a72cee161f9dad9261cec176&pid=1-s2.0-S2666053923000383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91684474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theoretical insights into gas sensing properties of MXene","authors":"Julaiba Tahsina Mazumder ,&nbsp;Ravindra Kumar Jha","doi":"10.1016/j.snr.2023.100174","DOIUrl":"10.1016/j.snr.2023.100174","url":null,"abstract":"<div><p>MXene has emerged as a prominent two-dimensional material with vast potential for diverse applications, garnering significant attention within the scientific community. This attention is due to its unique surface structure and its capacity for surface modification through functionalization, as well as its exceptional conductivity and elasticity. Extensive theoretical and experimental studies have been conducted to explore the gas sensing capabilities of various MXenes, and this review aims to present the latest advancements in this field through the lens of density functional theory. The review begins by providing a detailed explanation of the theoretical calculations involved, including the various available computational software options and the parameters considered, taking into account both cost and time complexity. The sensing properties of MXene derivatives are then comprehensively reviewed, categorized by their types (M₂X, M₃X₂, M₄X₃), and are discussed in terms of material properties, sensitivity, selectivity, and response time, amongst others. Furthermore, the prospects for these sensors are examined, focusing on their potential applications. Lastly, the review highlights future opportunities for theoretical research and the application of MXene materials in the development of cutting-edge devices. By presenting an overview of theoretical research and recent advancements, this review aims to provide valuable insights into this burgeoning field and pave the way for new avenues in gas detection research.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100174"},"PeriodicalIF":5.9,"publicationDate":"2023-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053923000371/pdfft?md5=5c9338f8d88d7a516a2c76b270aaf033&pid=1-s2.0-S2666053923000371-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46501891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetoresistance and magneto-plasmonic sensors for the detection of cancer biomarkers: A bibliometric analysis and recent advances","authors":"Fahad Usman ,&nbsp;Kamarul Hawari Ghazali ,&nbsp;Razali Muda ,&nbsp;Nasrul Hadi Johari ,&nbsp;John Ojur Dennis ,&nbsp;Nissren Tamam ,&nbsp;Abdelmoneim Sulieman ,&nbsp;Yuanfa Ji","doi":"10.1016/j.snr.2023.100172","DOIUrl":"10.1016/j.snr.2023.100172","url":null,"abstract":"<div><p>The conventional approaches to diagnosing cancer are expensive, often involve exposure to radiation, and struggle to identify early-stage lung cancer. As a result, the five-year survival rate is significantly reduced. Fortunately, promising alternatives using magnetoresistance (MR) and magneto-plasmonic sensors have emerged for swiftly, accurately, and inexpensively detecting cancer in its initial phases. These sensor technologies offer numerous advantages over their counterparts, such as minimal background noise, immunity to environmental influences, compatibility with nanofabrication methods, ability to detect multiple substances simultaneously, straightforward integration, high specificity, distinctive identifying capabilities, real-time monitoring, stability, label-free detection, and remarkable sensitivity for detecting individual molecules. Nevertheless, since the use of these techniques for cancer biomarker detection is relatively new, it is essential to conduct a bibliometric analysis and review recent literature to offer guidance to both early-career and established researchers in this domain. Consequently, this study performs a scientometric evaluation of the literature related to cancer biomarker detection using MR and magneto-plasmonic methods. The objective is to pinpoint current preferred techniques and challenges by examining statistics such as publication numbers, authors, countries, journals, and research interests. Furthermore, the paper also presents the latest advancements in MR and magneto-plasmonic sensors for cancer biomarker detection, with a focus on the last decade. In addition, an overview of the ongoing research in the field of MR and magneto-plasmonic sensors for detecting cancer biomarkers is highlighted. Finally, a summary on the level of current research including the significant accomplishments, challenges, and outlooks of MR and magneto-plasmonic sensors for the detection of cancer biomarkers are highlighted.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100172"},"PeriodicalIF":5.9,"publicationDate":"2023-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053923000358/pdfft?md5=729d0778df69c41be66d280865fa368a&pid=1-s2.0-S2666053923000358-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41761489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic microcarriers for sensing and cell expansion","authors":"Charlotte B.A. Stoffels ,&nbsp;Patrick Grysan ,&nbsp;Caroline Sion ,&nbsp;Rishabh Rastogi ,&nbsp;Matteo Beggiato ,&nbsp;Eric Olmos ,&nbsp;Sivashankar Krishnamoorthy","doi":"10.1016/j.snr.2023.100173","DOIUrl":"10.1016/j.snr.2023.100173","url":null,"abstract":"<div><p>Microcarriers (MCs, typically 50–200 µm) are promising growth supports for high-throughput cell expansion, with capability to overcome the limitations of surface area availability and nutrient access encountered by cell culture in 2D well plate configurations. Equipping MCs with in-built capability to sense molecular biomarkers is a key step forward to meet the emerging demands of personalized cell-based therapies. However, integrating sensing functionality into MCs is non-trivial due to fabrication limitations imposed by their large size, curved surfaces, and their suspension in fluid. If achieved, the sensor-integrated MCs should face further concerns of reduced stability and cytocompatibility during cell-culture. Here we demonstrate plasmonic microcarriers (PMCs) that integrate spectroscopic sensing and cell expansion functions through the deposition of gold nanoparticle (AuNP) assemblies on dextran-based MCs. Hydrogel characteristics of the dextran microcarriers was found to profoundly enhance the binding density and kinetics of AuNPs, as seen by attainment of saturated densities in few seconds, and at nanoparticle concentrations only twice that of the surface sites. The approaches to prepare PMCs are distinguished by simple, scalable routes, without need for sophisticated lab infrastructure. The capability of PMCs to act as spectroscopic transducers was demonstrated by surface-enhanced spectroscopic (SERS) detection of a model molecular probe.  The growth, proliferation and migration of human mesenchymal stem cells on the PMCs was found to be comparable to that of the uncoated MCs. The results pave the way to smart, multifunctional cell growth supports to interrogate, control and report cell behavior during culture.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100173"},"PeriodicalIF":5.9,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266605392300036X/pdfft?md5=2614ada1b8378ee2ae3c05a4382154a5&pid=1-s2.0-S266605392300036X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46666190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Au-NP-based colorimetric assay for sugar detection and quantification","authors":"Mawin JM Jimenez ,&nbsp;Andres Jaramillo-Botero ,&nbsp;Alba Avila","doi":"10.1016/j.snr.2023.100171","DOIUrl":"10.1016/j.snr.2023.100171","url":null,"abstract":"<div><p>Sugars have been associated with health problems such as metabolic syndrome, obesity, elevated triglycerides, and other features of metabolic disorders. Here, we report on the design and characterization of a highly sensitive gold nanoparticle (AuNP)-based assay for measuring sugars levels in fluids. AuNPs are functionalized with 4-mercaptobenzoic acid (4MBA) to modulate surface plasmonic resonance (SPR) frequency and fluorescent energy absorption by AuNPs, based on a fixed concentration of fluorescein introduced along with a fluid sample containing a determined quantity of sugar. We find the driving mechanism to be kinetic competition between fluorescein and saccharide which occupies the active carboxylic sites of the functional groups on the nanoparticle surface. This drives a colorimetric response in the 4MBA-AuNP solution, from orange to green, discernible to the naked eye. The aggregation of functionalized AuNPs as a result of increasing sugar concentration was confirmed via UV–vis spectroscopy and identified by a spectral peak of 620–675 nm. The limit of detection (LOD) was (55.8 ± 1.1) µM, with a highly repeatable calibration curve in the range of 0.09 – 2.45 mM. The assay's cost-effectiveness, speed, and simplicity open the door to multiple applications associated with detecting and quantifying sugars, including food science. The sensory platform was successfully tested in mixed sugar solutions to study the sensitivity capacity and applied to strawberry and kiwi freeze-dried samples. The results are validated by HPLC with refractive index detection.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100171"},"PeriodicalIF":5.9,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053923000346/pdfft?md5=a4c45a84784559708a89c002ad768d36&pid=1-s2.0-S2666053923000346-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46686373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of a microfluidic device for immunoaffinity-based isolation of circulating tumor cells with minimal clogging","authors":"Begum Sen-Dogan ,&nbsp;Ender Yildirim ,&nbsp;Sebnem Sahin ,&nbsp;Ebru Ozgur ,&nbsp;Ozge Zorlu ,&nbsp;Haluk Kulah","doi":"10.1016/j.snr.2023.100169","DOIUrl":"https://doi.org/10.1016/j.snr.2023.100169","url":null,"abstract":"<div><p>Combining bioaffinity-based techniques with microfluidics is an effective strategy for the selective isolation of rare circulating tumor cells (CTCs) among peripheral blood cells. In this scope, designing a microfluidic channel with high cell-surface interaction is crucial, which can be realized by increasing surface area <em>via</em> micropillars. In such microfluidic channels, the interpillar distance represents a critical design parameter, and the value is decided considering the trade-off between the possibility of clogging and CTC capture efficiency. In this study, a curvilinear microfluidic channel with a wide (150 µm) interpillar distance was developed to prevent clogging while maintaining high CTC capture efficiency. Computational fluid dynamics was used to compare the residence time of particles in the designed channels. For the proof-of-concept study, microfabricated channels were biofunctionalized for immunoaffinity-based isolation of CTCs, using anti-EpCAM antibodies. Enhanced CTC capture was enabled through the micropillars inside the channels helping the increased encounters between the cells and the antibody-functionalized surface. The curvilinear channel effectively isolated cells from MCF-7 breast cancer cell line among white blood cells, with more than 85% capture efficiency. The rate of non-specific binding of white blood cells remained below 20%. This study demonstrated the ability to increase the interactions between particles and surfaces without requiring a dense layout of the micropillars inside the microchannel, therefore minimizing the clogging possibility of the channel without sacrificing performance.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100169"},"PeriodicalIF":5.9,"publicationDate":"2023-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3209848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterning amyloid-β aggregation under the effect of acetylcholinesterase using a biological nanopore - an in vitro study","authors":"Nandhini Subramanian ,&nbsp;Brittany Watson ,&nbsp;Chen-Zhong Li ,&nbsp;Melissa Moss ,&nbsp;Chang Liu","doi":"10.1016/j.snr.2023.100170","DOIUrl":"https://doi.org/10.1016/j.snr.2023.100170","url":null,"abstract":"<div><p>Aggregation of amyloid-β peptide (Aβ) is hypothesized to be the primary cause of Alzheimer's disease (AD) progression. Aβ aggregation has been widely studied using conventional sensing tools like emission fluorescence, electron microscopy, mass spectroscopy, and circular dichroism. However, none of these techniques can provide cost-efficient, highly sensitive quantification of Aβ aggregation kinetics at the molecular level. Among the influences on Aβ aggregation of interest to disease progression is the acceleration of Aβ aggregation by acetylcholinesterase (AChE), which is present in the brain and inflicts the fast progression of disease due to its direct interaction with Aβ. In this work, we demonstrate the ability of a biological nanopore to map and quantify AChE accelerated aggregation of Aβ monomers to mixed oligomers and small soluble aggregates with single-molecule precision. This method will allow future work on testing direct and indirect effects of therapeutic drugs on AChE accelerated Aβ aggregation as well as disease prognosis.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100170"},"PeriodicalIF":5.9,"publicationDate":"2023-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10469531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1629255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protein biomarker detection in prostate cancer: A comprehensive review of electrochemical biosensors","authors":"Hashmatullah Nasimi ,&nbsp;Jonna Skov Madsen ,&nbsp;Ahmed H. Zedan ,&nbsp;Anders Malmendal ,&nbsp;Palle Jörn Sloth Osther ,&nbsp;Fatima AlZahra'a Alatraktchi","doi":"10.1016/j.snr.2023.100168","DOIUrl":"https://doi.org/10.1016/j.snr.2023.100168","url":null,"abstract":"<div><p>Rapid on-site electrochemical screening for prostate cancer (PCa) in the clinic holds the potential for early diagnosis of PCa patients. Currently, the accuracy of commonly used biomarkers is insufficient; however, several protein biomarker candidates show promise for diagnostic or prognostic patient screening. Among these candidates are Prostate specific antigen (PSA), Human glandular kallikrein 2 (hK2), Annexins 3 (Annex 3), Beta-2-microglobulin (β2M), Microseminoprotein-beta (MSMB), Serum amyloid A (SAA), and Engrailed-2 (EN2). This review provides an overview of the advancements in electrochemical biosensor development specifically targeting the detection of these seven biomarkers. The selection of these biomarkers was based on their suitability for protein-level detection using electrochemical sensors, as well as their presence in biological fluids. Moreover, we emphasize that relying solely on a single biomarker may not be sufficient to obtain precise diagnostic information. It is now widely accepted that panels of biomarkers measured in a multiplexed fashion are of greater clinical utility than single biomarkers. Therefore, we suggest that future research prioritize the utilization of multiplex electrochemical methods for measuring panels of protein biomarkers, offering a simple and efficient approach for regular PCa monitoring.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"6 ","pages":"Article 100168"},"PeriodicalIF":5.9,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1629254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}