Coral Salvo-Comino , Luis E. Alonso-Pastor , Clara Pérez-González , Stefano Pettinelli , Karina C. Núñez Carrero , Miguel Ángel Rodríguez-Pérez , María Luz Rodríguez-Méndez
{"title":"Impact of molecular structure and plasticization of PVC membranes in the response of solid-state ion-selective electrodes","authors":"Coral Salvo-Comino , Luis E. Alonso-Pastor , Clara Pérez-González , Stefano Pettinelli , Karina C. Núñez Carrero , Miguel Ángel Rodríguez-Pérez , María Luz Rodríguez-Méndez","doi":"10.1016/j.snr.2025.100301","DOIUrl":"10.1016/j.snr.2025.100301","url":null,"abstract":"<div><div>Potentiometric sensors are essential in various industries due to their high sensitivity, simplicity, and cost-effectiveness. However, enhancing sensitivity, largely dependent on membrane diffusion, remains challenging. This study investigates the role of polyvinyl chloride (PVC) molecular weight and plasticizer type on optimizing potentiometric sensor performance for tartaric acid detection. Two PVCs with different molecular weights (HIGH and LOW) and distinct plasticizers were evaluated. Results demonstrated that membranes plasticized with dibutyl sebacate, a linear plasticizer, exhibited high sensitivity (0.0239 mV/pX) and repeatability, achieving a lower limit of detection (LOD = 4.31 × 10⁻⁵ M) compared to those with a bulky plasticizer. Specifically, HIGH PVC combined with a linear plasticizer provided saturation-free measurements, ensuring greater accuracy in tartaric acid quantification. This research underscores the impact of plasticizer selection on sensor optimization, enhancing accuracy and reliability in food quality control. By establishing the relationship between PVC structure and plasticizer type, this study contributes to advancing sensor technology for improved food safety and quality assessment.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100301"},"PeriodicalIF":6.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecularly imprinted polypyrrole-modified screen-printed electrode: Toward a sensor for furfural derivatives determination","authors":"Daniele Merli , Alessandra Cutaia , Sofia Prosperi , Camilla Zanoni , Giancarla Alberti","doi":"10.1016/j.snr.2025.100299","DOIUrl":"10.1016/j.snr.2025.100299","url":null,"abstract":"<div><div>The increasing interest in determining furfural derivatives in food and beverages using low-cost and portable devices has driven our attention to the development of the here proposed voltammetric sensor. It is based on a screen-printed electrochemical cell whose graphite-ink working electrode is surface modified with electropolymerized molecularly imprinted polypyrrole (e-MIP), using 5-(hydroxymethyl)furan-2-carbaldehyde (HMF) as the template. Square wave voltammetry (SWV) is the selected analytical technique, exploiting the irreversible reduction peak of the carbonyl group at -1.7 V vs. Ag/AgCl pseudo reference electrode in 0.1 M phosphate buffer (PBS) at pH 8. The presence of the e-MIP on the working electrode surface enhances the sensitivity by lowering the detection limit of about one order of magnitude if compared with the bare electrode (LOD bare 93 µM; e-MIP 7 µM). The developed sensor is selective for the whole class of furfural-like compounds, allowing the determination of the total furanic aldehydes content. The sensor was applied to the analysis of synthetic solutions and real beverages, such as expresso coffee, obtaining good recovery percentages.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":6.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acoustic bubbles: Inducing cell interactions via trapping, patterning, and removal","authors":"Jonathan Faulkner , Mengren Wu , Madelyn Wicker , Yuan Gao","doi":"10.1016/j.snr.2025.100294","DOIUrl":"10.1016/j.snr.2025.100294","url":null,"abstract":"<div><div>Studying cell-cell interactions is crucial for understanding biological processes and advancing drug development. Recent advancements in microfluidic technology allow cell-cell interaction experiments to be performed on-chip, enabling the study of specific interactions within a highly defined microenvironment. Acoustic bubbles, tiny bubbles excited by an external acoustic source, are clean, biocompatible, and effective tools for manipulating fluids and particles at the microscale. This research presents a novel microfluidic approach using acoustic bubbles to study cell-cell interactions. It focuses on generating, controlling, and removing acoustic bubbles within a microfluidic device to manipulate particles and cells and induce their interaction. In this work, a microbubble is passively generated to separate the channels containing two different types of particles and cells. Under the control of an acoustic field, particles and cells are trapped on either side of the microbubble. The microbubble is then removed to enable interaction between the trapped particles and cells. The impact of channel geometry on bubble stability is quantitatively investigated to optimize the device's performance. Additionally, the successful demonstration of on-chip cell interactions highlights the rapid and straightforward operation of this method. This innovative approach holds great potential for studying specific cell interactions to gain biological insights into disease progression and serves as an effective tool for drug testing.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100294"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junting Liang , Dihan Duan , Lubing Sun , Jinming Li , Mengke Wang , Ziyi Chang , Rick F. Thorne , Chuanliang Chen , Demin Duan
{"title":"High-sensitivity colorimetric sensor based on oxidase-like Mn3O4 nanozyme for Cys detection","authors":"Junting Liang , Dihan Duan , Lubing Sun , Jinming Li , Mengke Wang , Ziyi Chang , Rick F. Thorne , Chuanliang Chen , Demin Duan","doi":"10.1016/j.snr.2025.100296","DOIUrl":"10.1016/j.snr.2025.100296","url":null,"abstract":"<div><div>Cysteine (Cys) is essential for numerous physiological processes and play a vital role in disease diagnostics, underscoring the necessity for effective and sensitive detection techniques for Cys. In this research, we introduce an ultrasensitive colorimetric approach based on oxidase-like Mn<sub>3</sub>O<sub>4</sub> nanozymes. The nanozymes were synthesized through a straightforward wet chemical method and displayed a flower-like globular morphology, showing remarkable oxidase-like catalytic performance with a low K<sub>m</sub> value of 0.1896 mM and a high V<sub>max</sub> of 2.8 × 10⁻⁷ M s⁻¹. By leveraging the outstanding catalytic activities of Mn<sub>3</sub>O<sub>4</sub> nanozymes and optimizing the TMB concentration, we developed a novel and sensitive colorimetric sensor for Cys detection. Experimental results indicated a detection range for Cys between 0.5 and 45 μM, with a lower limit of detection (LOD) of 0.02636 μM. This established method for detecting Cys reveals significant promise for the advancement of effective sensing systems.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100296"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Selvaraj Chinnathambi , Mohammad Saghafi , Suryasnata Tripathy , Frans P. Widdershoven , Serge G. Lemay
{"title":"High-frequency electrochemical impedance measurements of self-assembled monolayer formation using CMOS-based nanocapacitor arrays","authors":"Selvaraj Chinnathambi , Mohammad Saghafi , Suryasnata Tripathy , Frans P. Widdershoven , Serge G. Lemay","doi":"10.1016/j.snr.2025.100293","DOIUrl":"10.1016/j.snr.2025.100293","url":null,"abstract":"<div><div>Electrochemical impedance spectroscopy is mostly sensitive to the state of the electrode surface at low frequencies, where the electrical properties of the surrounding electrolyte do not yet dominate the response. The available frequency range increases linearly with the inverse electrode size, such that miniaturized electrodes offer the ability to probe the surface at higher frequencies. Measuring the AC response of miniaturized electrodes is however highly challenging due to the parasitic capacitance of the interconnects. Here we employ nanoelectrodes integrated with readout circuitry on the same complementary metal-oxide-semiconductor (CMOS) chip to conduct measurements over a frequency range from 1.6 MHz to 50 MHz. We report the ability to probe the formation of self-assembled monolayers at frequency above 10 MHz in phosphate-buffered saline solution.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100293"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alba López-Palacios , María Nieves Aranda , Isabel Caballos , Andy Hernández-Montoto , Eva Calabuig , María Dolores Gómez-Ruiz , María Ángeles Tormo-Mas , Javier Pemán , Félix Sancenón , Ramón Martínez-Máñez , Elena Aznar , Estela Climent
{"title":"SARS-COV-2 viral RNA detection through oligonucleotide-capped nanoporous anodic alumina supports.","authors":"Alba López-Palacios , María Nieves Aranda , Isabel Caballos , Andy Hernández-Montoto , Eva Calabuig , María Dolores Gómez-Ruiz , María Ángeles Tormo-Mas , Javier Pemán , Félix Sancenón , Ramón Martínez-Máñez , Elena Aznar , Estela Climent","doi":"10.1016/j.snr.2025.100298","DOIUrl":"10.1016/j.snr.2025.100298","url":null,"abstract":"<div><div>We describe herein a sensor containing nanoporous anodic alumina (NAA) as sensing platform to identify SARS-COV-2 RNA using a gating mechanism. The system is based on NAA that contains a fluorescent dye (Rhodamine B; RhB) and is capped with an oligonucleotide sequence that hybridize specifically a region of SARS-CoV-2 genome. In the presence of RNA from SARS-COV-2 virus, the oligonucleotide of the surface is displaced, uncapping the pores, and producing a delivery of RhB. The detection of the virus is achieved measuring the fluorescence of the fluorophore. The nanosensor demonstrates to be highly sensitive and selective in aqueous buffers and in biological media, having a limit of detection (LOD) of 50 ± 30 copies mL<sup>-1</sup> of SARS-CoV-2 RNA extracted from patients. Moreover, preliminary results using 18 real nasopharyngeal swab samples indicate the potential of the system to differentiate between infected and non-infected patients. Compared to the conventional RT-PCR method, in our system there is no need for sample pretreatment or RNA isolation, providing diagnostic outcomes within 60 mins while maintaining a high level of reliability.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100298"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mina Adampourezare , Karim Asadpour-Zeynali , Miguel de la Guardia , Jafar Ezzati Nazhad Dolatabadi
{"title":"The design of paper-based electroanalytical microfluidic device coupled with post-synthesized molecularly imprinted polymers (rGO/Au@Ag2S/PANI/polyacrylamide) for the detection of streptomycin","authors":"Mina Adampourezare , Karim Asadpour-Zeynali , Miguel de la Guardia , Jafar Ezzati Nazhad Dolatabadi","doi":"10.1016/j.snr.2025.100297","DOIUrl":"10.1016/j.snr.2025.100297","url":null,"abstract":"<div><div>A highly sensitive three-electrode paper-based microfluidic device has been developed for determinate of streptomycin. Ag/GQD/chitosan nano ink was used to construct reference and counter electrodes and Au@Ag<sub>2</sub>S/GQD/chitosan nano ink was used to construct working electrode. rGO/Au@Ag<sub>2</sub>S/anline nano-sheets were encapsulated with acrylamide as a functional monomer to create novel nano-sized molecular imprinted polymer (MIP) and was used for the modification of electrode working.</div><div>Paper substrate was modified for forming a hydrophobic region around the hydrophilic region on the filter paper and were suitable for the evaluation of target molecules in aqueous environment. All steps of making the MIP and non-imprinted polymer (NIP) sensors are illustrated by FE-SEM, Energy-dispersive X-ray spectroscopy (EDS), and FT-IR. CV and DPV techniques were applied for the measurement of the streptomycin. The fabricated device showed high sensitivity toward streptomycin, which was due to synergistic effects between catalytic properties, electrical conductivity of rGO/Au@Ag<sub>2</sub>S/anline /Polyacrylamide, and considerably increased numbers of imprinted sites. Using these techniques, streptomycin was determined in the linear range of 1pM to 1 μM with a limit of detection of 0.9 × 10<sup>−12</sup> M (Ip (μA) = -2.075x + 31.575, R<sup>2</sup>= 0.9983). The device provided good cyclic stability (RSD < 0.05), high reproducibility and selectivity (RSD < 0.05 obtained from five calibration plots) with a good repeatability (for both milk and serum samples, RSD < 0.05). Finally, it should be noted that this device showed a good analytical performance for the detection of streptomycin in milk and serum samples.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100297"},"PeriodicalIF":6.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143348597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehr Un Nisa , Asim Yaqub , Muhammad Hashim Khan , Fatima Yaseen , Shahaab Jilani , Huma Ajab , Noor S. Shah , Abdulaziz Al-Anazi
{"title":"Nanotechnology-driven electrochemical neurotransmitter sensing as a fundamental approach towards improving diagnostics and therapeutics: A review","authors":"Mehr Un Nisa , Asim Yaqub , Muhammad Hashim Khan , Fatima Yaseen , Shahaab Jilani , Huma Ajab , Noor S. Shah , Abdulaziz Al-Anazi","doi":"10.1016/j.snr.2025.100292","DOIUrl":"10.1016/j.snr.2025.100292","url":null,"abstract":"<div><div>Neurotransmitters (NTs) are endogenous chemical messengers that have a wide range of key roles throughout the nervous system. It is an important signal medium of neural transfers and equally influence physiological conditions. While, abnormal levels are linked with psychotic, physical, and neurodegenerative disorders such as ADHD, autism and tourette syndrome. Therefore, their accurate and sensitive detection is of great importance. Traditional methods of NTs detection are limited to complex sample processing, high cost, and real-time diagnostics. However, an electrochemical sensing has been extensively utilized in the past few years for NTs detection, which shows better sensitivity, selectivity, and capacity for operation in complicated biological environments, constitutes a promising approach. In this review, the most successful and favorable electrochemical sensors based on innovations in electrode materials and nanostructured surfaces having substantial improvement in the analytical performances and overcoming challenges followed by future prospects in NTs detection have been reported. This review additionally looks at the progress accomplished in biosensors for neurotransmitter evaluations in microfluidic lab-on-a-chip systems, smart wearable devices and other advanced technologies for the goal of real-time, portable diagnosis in neuroscience and clinics. These advances are paving the way for more effective applications of electrochemical sensing in neuroscience and clinical diagnostics.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100292"},"PeriodicalIF":6.5,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Laccase-mimicking Cu@CDs-enabled bioinspired immunosensor using CuO nanoparticles for voltametric detection of serum thyroxine level in patients with hyperthyroidism or hypothyroidism","authors":"Mohammad Ali Farzin, Seyed Morteza Naghib","doi":"10.1016/j.snr.2025.100291","DOIUrl":"10.1016/j.snr.2025.100291","url":null,"abstract":"<div><div>In this study, for the first time, a voltammetric bioinspired immunosensor was fabricated to determine free thyroxine (T4) based on the blocking nanozyme-dependent signaling pathway through masking the active sites of the laccase-mimicking Cu-doped CDs (Cu@CDs). The focus shifted to monitoring a vital biomarker for thyroid diseases using a metal-containing nanozyme, showcasing high potential to catalyze the oxidation of phenolic compounds. Thanks to the active sites of Cu@CD nanozyme, the electrocatalytic oxidation of hydroquinone (HQ), as a signal probe, was performed at a high rate. The CuO nanostructures also increased the conductivity as well as specific surface area of electrode. Despite signal amplification of HQ, the binding of antibody to carbon dot reduced enzymatic activity of nanozyme and subsequently resulted in a considerable decrease in the current intensity of GCE/CuO nanoparticles/Cu@CDs-HQ. The bioconjugation of T4 with anti-T4 antibody resulted in the further coverage of the active sites of nanozyme. Therefore, the current of GCE/CuO nanoparticles/Cu@CDs-HQ/Ab decreased proportionally to the T4 concentration in a DLR (dynamic linear range) of 10.0 pM-8.0 nM which comprises the reference range for free T4 (11.5–22.7 pM). The LOD (limit of detection) of 2.8 pM, was lower compared to the values reported in previous studies. Furthermore, this study exhibited the key finding that the highly specific interaction of T4 with immobilized antibody led to a remarkable diminish in the immunosensor signal compared to the interferers. More importantly, spike-recovery studies demonstrated the high potential of the designed immunosensor to detect serum T4 levels in patients with hyperthyroidism or hypothyroidism.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100291"},"PeriodicalIF":6.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Un Yup Lee , Min Young Kim , Kyu Hyoung Lee , Sol Han , Seung Yong Lee , Ali Mirzaei , Sun-Woo Choi , Myung Sik Choi , Changhyun Jin , Jeong Yun Hwang
{"title":"Surface reaction mechanism and characteristics of 2-dimensional TiO2 and 0-dimensional Ag nanocomposites specialized for H2S gas sensing at room temperature","authors":"Un Yup Lee , Min Young Kim , Kyu Hyoung Lee , Sol Han , Seung Yong Lee , Ali Mirzaei , Sun-Woo Choi , Myung Sik Choi , Changhyun Jin , Jeong Yun Hwang","doi":"10.1016/j.snr.2025.100290","DOIUrl":"10.1016/j.snr.2025.100290","url":null,"abstract":"<div><div>In this study, we provide the synthesis of pristine TiO<sub>2</sub> nanosheets (NSs) via a simple flux method and their decoration with Ag nanoparticles (NPs) using a hydrothermal method for room-temperature H<sub>2</sub>S gas sensing. Gas sensing experiments were conducted at 25 °C, evaluating the response of the Ag NPs-decorated TiO<sub>2</sub> NSs to various gases. Results demonstrated the gas sensor exhibited a superior response to H<sub>2</sub>S gas compared to other gases, outperforming the pristine TiO<sub>2</sub> NSs gas sensor. Additionally, its response to H<sub>2</sub>S gas surpassed that of the TiO<sub>2</sub> NSs gas sensor, highlighting the promising role of Ag decoration for H<sub>2</sub>S gas detection. Sensing behavior in humid atmosphere was also investigated. The improved H<sub>2</sub>S gas sensing performance can be attributed to the catalytic effect of Ag, the formation of Ag/TiO<sub>2</sub> heterojunctions, and the large surface area of the TiO<sub>2</sub> NSs. This study introduces a straightforward approach to enhance the gas sensing capabilities of metal oxide NSs.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100290"},"PeriodicalIF":6.5,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}