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Room temperature-based hydrogen gas sensing over Laser-Induced Graphene electrode supported Pt nanoparticles for low LOD 基于室温的激光诱导石墨烯电极氢气传感,支持铂纳米粒子,实现低 LOD
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-09-30 DOI: 10.1016/j.snr.2024.100247
Minseob Lim, Jun Young Kim, Hyunji Kang, Tae Woong Yun, Hong-Baek Cho, Yong-Ho Choa
{"title":"Room temperature-based hydrogen gas sensing over Laser-Induced Graphene electrode supported Pt nanoparticles for low LOD","authors":"Minseob Lim,&nbsp;Jun Young Kim,&nbsp;Hyunji Kang,&nbsp;Tae Woong Yun,&nbsp;Hong-Baek Cho,&nbsp;Yong-Ho Choa","doi":"10.1016/j.snr.2024.100247","DOIUrl":"10.1016/j.snr.2024.100247","url":null,"abstract":"<div><div>This study introduces a novel hydrogen sensor that operates efficiently at room temperature with high sensitivity and selectivity. This sensor was created by utilizing a platinum on laser-induced graphene (Pt/LIG) structure. The synthesis process involves the creation of highly crystalline graphene with a large surface area, which serves as an optimal support for nanosized pt catalysts. Post-synthesized Pt nanoparticles were dispersed on the surface of the LIG electrode and envisaged for the hydrogen gas sensing property under ambient conditions without a heating or sensor device. Analysis showed that the Pt nanoparticles are uniquely characterized by their narrow size distribution of less than 5 nm and their homogeneous deposition on the LIG substrate, which itself exhibits a substantial specific surface area of 187.4 m²/g. This configuration enables the sensor to achieve a very low limit for detection of hydrogen to 200 ppb. Moreover, the sensor demonstrates exceptional performance attributes, including high sensitivity, excellent linearity, and remarkable cycle stability over 50 cycles. The synergy between the high surface area of the LIG and the catalytic activity of the Pt nanoparticles facilitates the detection of hydrogen at room temperature. This study contributes significantly to the field of gas sensing technology, particularly in applications requiring accurate and reliable hydrogen detection at ambient conditions.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100247"},"PeriodicalIF":6.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
H2S gas sensing enhancement of Au-decorated SnO2 nanospheres synthesized using hydrothermal and microwave methods 采用水热法和微波法合成的金装饰二氧化锡纳米球对 H2S 气体传感的增强作用
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-09-30 DOI: 10.1016/j.snr.2024.100246
Jiyeon Shin , Jeong Yun Hwang , Changyu Kim , Jimyeong Park , Jong Wook Roh , Sun-Woo Choi , Changhyun Jin , Myung Sik Choi
{"title":"H2S gas sensing enhancement of Au-decorated SnO2 nanospheres synthesized using hydrothermal and microwave methods","authors":"Jiyeon Shin ,&nbsp;Jeong Yun Hwang ,&nbsp;Changyu Kim ,&nbsp;Jimyeong Park ,&nbsp;Jong Wook Roh ,&nbsp;Sun-Woo Choi ,&nbsp;Changhyun Jin ,&nbsp;Myung Sik Choi","doi":"10.1016/j.snr.2024.100246","DOIUrl":"10.1016/j.snr.2024.100246","url":null,"abstract":"<div><div>In this study, non-oriented Au-decorated SnO<sub>2</sub> nanospheres (NSs) were synthesized using hydrothermal and microwave methods. The SnO<sub>2</sub> spheres ranged from hundreds of nanometers to a microscale. The oxygen vacancy on the surface increased after Au adsorption on the surface of the SnO<sub>2</sub> NSs, ultimately showing a synergistic effect with the spillover effect of the existing Au catalyst. Specifically, at 100 °C and 200 °C, the response to 10 ppm H<sub>2</sub>S gas improved to 16.14 and 46.81, respectively. These gas sensing effects were approached individually by being divided into two disadvantages (oxygen adsorption and homojunction) and five advantages (oxygen vacancies, spill-over, surface area, H<sub>2</sub>S + SnO<sub>2</sub> reaction, and H<sub>2</sub>S + O<sub>2</sub> reaction). Based on this reference, we investigated SnO<sub>2</sub> NSs of various sizes and functions by adjusting the process variables.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100246"},"PeriodicalIF":6.5,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142420519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Single-capsid identification of full and empty status of recombinant adeno-associated viruses via resistive pulse sensing 通过电阻脉冲传感技术识别重组腺相关病毒的完整和空白状态的单囊壳
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-09-06 DOI: 10.1016/j.snr.2024.100242
Hanna T. Nguyen , Farhad Shiri , Matthew Verber , Collin McKinney , Junseo Choi , Sunggook Park , Javan Surtan , Simna Saraswathi Prasannakumari , Kimberly D. Ritola , Steven A. Soper
{"title":"Single-capsid identification of full and empty status of recombinant adeno-associated viruses via resistive pulse sensing","authors":"Hanna T. Nguyen ,&nbsp;Farhad Shiri ,&nbsp;Matthew Verber ,&nbsp;Collin McKinney ,&nbsp;Junseo Choi ,&nbsp;Sunggook Park ,&nbsp;Javan Surtan ,&nbsp;Simna Saraswathi Prasannakumari ,&nbsp;Kimberly D. Ritola ,&nbsp;Steven A. Soper","doi":"10.1016/j.snr.2024.100242","DOIUrl":"10.1016/j.snr.2024.100242","url":null,"abstract":"<div><p>Recombinant adeno-associated viruses (rAAVs) are promising vectors for gene therapy, but their production is hampered by the presence of empty capsids, escalating costs and diminishing safety and efficacy. Traditional methods for assessing capsid content status lack adaptability to in-line production workflows. The lack of in-line methods to monitor rAAV production quality limits efforts seeking to improve production efficiency and subsequent <em>in vivo</em> performance. This study introduces a dual in-plane nanopore sensor fabricated in thermoplastics via replication, which can offer near real-time sensing for potential integration into gene therapy production lines and scalability for high-scale manufacturing at low cost. By leveraging differences in surface charge density and internal capsid content, coupled nanoscale electrophoresis and resistive pulse sensing enabled label-free identification of individual full and empty capsids using supervised machine learning with a neural network. This single-capsid analysis approach may also offer insights into rAAV-host cell interactions and population heterogeneity. We demonstrated using rAAV9 as an example due to its wide use in gene therapies, but establishment of the methodology as delineated in this manuscript will make the technology applicable to other vectors, such as lentiviruses and adenoviruses.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100242"},"PeriodicalIF":6.5,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000584/pdfft?md5=fe6a8138843bd34be0eae0414d942b8e&pid=1-s2.0-S2666053924000584-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142163635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A critical review: Advanced electrochemical analysis based on nanoscale scanning electrochemical microscopy 重要评论:基于纳米级扫描电化学显微镜的高级电化学分析
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-09-05 DOI: 10.1016/j.snr.2024.100243
Je Hyun Bae
{"title":"A critical review: Advanced electrochemical analysis based on nanoscale scanning electrochemical microscopy","authors":"Je Hyun Bae","doi":"10.1016/j.snr.2024.100243","DOIUrl":"10.1016/j.snr.2024.100243","url":null,"abstract":"<div><p>Electroanalysis is a type of analytical method used to study an analyte by measuring its electrical properties in an electrochemical cell. It has been widely used because it is a relatively simple and inexpensive technique, has a low detection limit and is able to measure original electrical signals. Electroanalysis has advanced with the development of nanotechnology, offering new opportunities for analysis. Scanning electrochemical microscopy (SECM) is a probe-based surface analysis instrument that enables real-time surface and interfacial analysis with spatial resolution in an electrochemical environment. In this review, we focus on the use of nanoscale SECM for improved electroanalysis. After introducing the fabrication, characterization, and modification of nanoelectrodes, which are the key to enabling nanoscale SECM, we introduce the instrumentation and fundamental principles of SECM. Several examples are provided to illustrate the advanced electroanalysis of photo-electrocatalysts and biosystems based on nanoscale SECM, which will be utilized as a more powerful electroanalytical tool in the future when combined with other analytical instruments.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100243"},"PeriodicalIF":6.5,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000596/pdfft?md5=16217f9f1bc1a5627f1ead0b2e0e8e3d&pid=1-s2.0-S2666053924000596-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Electrochemical detection of heavy metal ions adsorbed on microplastics with varying surface charges 电化学检测表面电荷不同的微塑料上吸附的重金属离子
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-08-30 DOI: 10.1016/j.snr.2024.100241
Sachintha D. Illesinghe, Vignesh Sundaresan
{"title":"Electrochemical detection of heavy metal ions adsorbed on microplastics with varying surface charges","authors":"Sachintha D. Illesinghe,&nbsp;Vignesh Sundaresan","doi":"10.1016/j.snr.2024.100241","DOIUrl":"10.1016/j.snr.2024.100241","url":null,"abstract":"<div><p>Microplastics (MPs) are global pollutants found in various environmental compartments, including oceans, freshwater bodies, soils, and air. Their persistence and potential to adsorb heavy metals raise significant concerns regarding water quality and ecosystem health. Understanding the interaction between MPs and heavy metals is crucial for assessing environmental risks and developing remediation strategies. In this study, we use electroanalytical techniques to investigate the adsorption of heavy metal ions— Cr³⁺, Zn²⁺, and Ni²⁺—by polystyrene MPs with different surface functionalizations: carboxyl, amino, and unfunctionalized. We conducted electrodeposition of metal ions on a gold ultramicroelectrode both before and after mixing with MPs. Following this, we used anodic stripping voltammetry to measure the change in the electrodeposited charge (ΔQ). This charge difference, observed before and after the interaction of ions with MPs, was analyzed to understand the adsorption kinetics and dynamics. Our experiments revealed that carboxyl-functionalized MPs exhibited the highest ΔQ due to strong electrostatic attraction with the metal ions, with values 1.67 ×, 1.50 ×, and 1.22 × greater than those for amino-MPs for Cr³⁺, Zn²⁺, and Ni²⁺, respectively. Amino-MPs displayed considerable ion adsorption, suggesting poor electrostatic repulsion between two positively charged entities. Interestingly, unfunctionalized MPs, which had a negative surface charge similar to carboxyl-MPs, showed different adsorption characteristics, with lower ΔQ values for all metal ions. This study underscores the significant role of surface functionalization on the adsorption efficiency and kinetics of heavy metal ions by MPs. It also demonstrates the utility of electroanalytical techniques in understanding metal ion-MP interactions, offering insights into potential environmental impacts and remediation strategies, as well as in developing electrochemical sensors for detecting heavy metal ions adsorbed on MPs.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100241"},"PeriodicalIF":6.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000572/pdfft?md5=404d6c1773b095ba571af67154ce15a6&pid=1-s2.0-S2666053924000572-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142147966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chitosan-coated iron(III) oxide nanoparticles and tungsten disulfide quantum dots-immobilized Fiber-based WaveFlex Biosensor for Staphylococcus Aureus bacterial detection in real food samples 壳聚糖涂层氧化铁(III)纳米粒子和二硫化钨量子点固定化纤维型 WaveFlex 生物传感器用于检测真实食品样品中的金黄色葡萄球菌细菌
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-08-30 DOI: 10.1016/j.snr.2024.100239
Xianzheng Lang , Ragini Singh , Qinghua Zeng , Jun Li , Daniele Tosi , Jan Nedoma , Carlos Marques , Bingyuan Zhang , Santosh Kumar
{"title":"Chitosan-coated iron(III) oxide nanoparticles and tungsten disulfide quantum dots-immobilized Fiber-based WaveFlex Biosensor for Staphylococcus Aureus bacterial detection in real food samples","authors":"Xianzheng Lang ,&nbsp;Ragini Singh ,&nbsp;Qinghua Zeng ,&nbsp;Jun Li ,&nbsp;Daniele Tosi ,&nbsp;Jan Nedoma ,&nbsp;Carlos Marques ,&nbsp;Bingyuan Zhang ,&nbsp;Santosh Kumar","doi":"10.1016/j.snr.2024.100239","DOIUrl":"10.1016/j.snr.2024.100239","url":null,"abstract":"<div><p>The research proposes and investigates an extraordinarily sensitive, label-free WaveFlex biosensor utilizing optical fiber technology for the detection of <em>Staphylococcus aureus</em> (<em>S. aureus</em>), a common foodborne bacterium. The WaveFlex biosensor (plasmon Wave based Flexible optical fiber Biosensor) is based on a novel flexible tapered single-mode fiber structure, utilizing gold nanoparticles (AuNPs) to trigger the phenomenon of localized surface plasmon resonance (LSPR). Additionally, the sensitivity is further enhanced using chitosan-coated iron(III) oxide nanoparticles (Fe<sub>3</sub>O<sub>4</sub>–CS NPs) and tungsten disulfide quantum dots (WS<sub>2</sub>-QDs). The optical fiber surface is functionalized with antibodies to achieve specific detection of <em>S. aureus</em>. For <em>S. aureus</em> concentrations at 1 × 10<sup>8</sup> CFU/mL (colony-forming units per milliliter), the sensor's maximum sensitivity of 2.74 nm/lg (CFU/mL), and a detection limit (LOD) of 6.67 CFU/mL. This ultra-sensitive biosensor holds great potential for widespread applications in various fields, including disease detection, medical diagnostics, and food safety inspection.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100239"},"PeriodicalIF":6.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000559/pdfft?md5=da349f34f60feee305d07580b1a313ec&pid=1-s2.0-S2666053924000559-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142094604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of molecular redox states on silica surfaces using shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) with various shell thicknesses 利用不同外壳厚度的外壳隔离纳米粒子增强拉曼光谱(SHINERS)表征二氧化硅表面的分子氧化还原态
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-08-30 DOI: 10.1016/j.snr.2024.100240
Ju-Young Kim , Sang Bin Yoon , Sehee Lee , Jungmin Park , Hyejin Park , Sukyeong Hwang , Hyon Bin Na , Kyungwon Kwak , Minhaeng Cho
{"title":"Characterization of molecular redox states on silica surfaces using shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) with various shell thicknesses","authors":"Ju-Young Kim ,&nbsp;Sang Bin Yoon ,&nbsp;Sehee Lee ,&nbsp;Jungmin Park ,&nbsp;Hyejin Park ,&nbsp;Sukyeong Hwang ,&nbsp;Hyon Bin Na ,&nbsp;Kyungwon Kwak ,&nbsp;Minhaeng Cho","doi":"10.1016/j.snr.2024.100240","DOIUrl":"10.1016/j.snr.2024.100240","url":null,"abstract":"<div><p>Understanding the molecular redox state is crucial for investigating chemical activities involving electron exchange, particularly in optical electrochemistry. Methyl viologen (MV) is commonly employed as a redox mediator and electron acceptor, exhibiting three distinct redox states (MV<sup>0</sup>, MV<sup>+</sup>, and MV<sup>2+</sup>), each characterized by a unique molecular structure and Raman spectrum. Utilizing surface-enhanced Raman spectroscopy (SERS), we explore the discrete molecular redox states of MV on shell-isolated nanoparticles (SHINs), which are gold nanoparticles (AuNPs) coated with silica shells of varying thicknesses, ranging from 1 to 10 nm. Our study, employing 532 nm excitation, reveals that all three redox forms of MV are sporadically observed on the metallic surfaces of AuNPs. However, the radical cation (MV<sup>+</sup>) state is predominantly detected on the silica surfaces of the SHINs, irrespective of the shell thickness. This consistency across different shell thicknesses suggests that electromagnetic (EM) effect predominantly contributes to the Raman enhancement in shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS), rather than enhancement via electron transfer. If electron transfer were induced by laser excitation, varying redox species would likely appear dependent on shell thickness. Given the absence of external perturbation such as applied potential or reducing agents, we believe our findings can provide a crucial reference for future studies using MV as a redox state-sensing probe. Furthermore, our results demonstrate the efficacy of SHINs as a robust nano-sensing platform that efficiently prevents direct contact with the metallic surface and unwanted reactions.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100240"},"PeriodicalIF":6.5,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000560/pdfft?md5=b9b17edfff6e225310eb7f090e36de18&pid=1-s2.0-S2666053924000560-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142129565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A multiple method for sensitively detecting 17 highly infectious bacteria and viruses with distinguished melting peaks 灵敏检测 17 种高传染性细菌和病毒的多重方法,熔融峰各不相同
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-08-24 DOI: 10.1016/j.snr.2024.100237
Jianping Wang, Ming Wang, Junpeng Fu, Xiaohui He
{"title":"A multiple method for sensitively detecting 17 highly infectious bacteria and viruses with distinguished melting peaks","authors":"Jianping Wang,&nbsp;Ming Wang,&nbsp;Junpeng Fu,&nbsp;Xiaohui He","doi":"10.1016/j.snr.2024.100237","DOIUrl":"10.1016/j.snr.2024.100237","url":null,"abstract":"<div><h3>Background</h3><p>Multiple fluorescence PCR was used to facilitate the low cost detection of such infectious pathogens. The melting curve combined with the fluorescence PCR method was used to detect more targets. The characteristic melting peaks of the melting curve formed by the melting probe were complementary to the fluorescent probes and more targets could be identified by the different melting peaks in one fluorescence channel.</p></div><div><h3>Results</h3><p>The most optimal conditions were confirmed by optimizing the experimental conditions, such as the concentration of fluorescent probes and the melting probe, which is crucial for forming the characteristic melting peaks. LOD, multiplex performance and sample detection performance were evaluated separately. According to the results, the LOD of all targets was as low as five or ten copies/tube, excepting for <em>Zaire type Ebola virus</em>, whose LOD is 25 copies/tube. The method could simultaneously detected more targets, even up to eight bacteria or nine viruses and the result was accurate. In 173 clinical or real-world samples, the result was in keeping with the expected or clinical results, excepting for one case of the soil sample, and the consistency rate of the detection results reached 99.42%.</p></div><div><h3>Significance</h3><p>We achieved a novel method for multiple detection of up to 17 highly infectious pathogenic microorganisms and performed well in real sample detection, and the results were consistent with the expected or clinical results. We provided a new detection tool for disease prevention, control centers and clinical practice.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100237"},"PeriodicalIF":6.5,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000535/pdfft?md5=9836d2278272d4383e8be2f8c613433c&pid=1-s2.0-S2666053924000535-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142083896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proposition of a phagosensor with a unique Teseptimavirus SAL_R1S on a carbon nanotube platform for efficient detection of typhoid pathogen 提出一种在碳纳米管平台上使用独特的 Teseptimavirus SAL_R1S 的吞噬传感器,用于高效检测伤寒病原体
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-08-23 DOI: 10.1016/j.snr.2024.100238
Md Hasibul Hassan , Md. Romzan Ali , Md. Arifur Rahman , Anamica Hossain , Sunjida Afrin , Md. Abdul Khaleque , Md. Anwar Hossain , Md. Zaved Hossain Khan , Munawar Sultana
{"title":"Proposition of a phagosensor with a unique Teseptimavirus SAL_R1S on a carbon nanotube platform for efficient detection of typhoid pathogen","authors":"Md Hasibul Hassan ,&nbsp;Md. Romzan Ali ,&nbsp;Md. Arifur Rahman ,&nbsp;Anamica Hossain ,&nbsp;Sunjida Afrin ,&nbsp;Md. Abdul Khaleque ,&nbsp;Md. Anwar Hossain ,&nbsp;Md. Zaved Hossain Khan ,&nbsp;Munawar Sultana","doi":"10.1016/j.snr.2024.100238","DOIUrl":"10.1016/j.snr.2024.100238","url":null,"abstract":"<div><p>The detection of the typhoid pathogen, <em>Salmonella enterica</em> serotype Typhi (<em>S</em>. Typhi), holds massive clinical, public health, and epidemiological significance around the globe. Conventional diagnosis relies on bacterial isolation having a set of challenges when it comes to accurate detection, therapeutic intervention and disease management. Substantial reviews and reports exist on the advantages of bacteriophage-based biosensors (phagosensors) concerning <em>Salmonella</em>. However, phagosensor for <em>Salmonella</em> Typhi point of care detection at a lower limit of detection (LOD) has yet to be reported. This study is the earliest endeavor to develop a multi-wall carbon nanotubes (MWCNTs) based electrochemical phagosensor utilizing a unique bacteriophage SAL_R1S as a biomolecular recognition element, selectively binding <em>S.</em> Typhi DMS_A1 at LOD of 1 CFU/ml. <em>S.</em> Typhi DMS_A1, retrieved from patient's blood, consists of 10 pathogenicity islands and a wide range of efflux pump genes in its whole genome, which has not yet been documented for <em>Salmonella</em>. Subsequent screening for its specific bacteriophage from a sewage sample pinpointed the phage SAL_R1S of class Caudoviricetes, family <em>Autographiviridae</em> and genus <em>Teseptimavirus</em>. The whole genome- and tail-fiber protein- based alignment was close to <em>Salmonella</em> phage Vi06 covering 88.8 % and 90 % similarity, respectively. SAL_R1S exclusively binds <em>S.</em> Typhi in a specific manner and also possess excellent genetic feature as a candidate for developing a highly sensitive electrochemical phagosensor. Therefore, it was covalently immobilized onto a modified SPE/MWCNT/PANI-based electrode surface, allowing charge-directed, oriented immobilization which then confirmed through scanning electron microscopy. The electrode surface was featured via field emission scanning electron microscopy, electrochemical impedance spectroscopy, and cyclic voltammetry. The pathogen detection process of the phagosensor is quick (∼ 20 min). It has exceptional selectivity for typhoid pathogens from blood, wastewater or within mixed populations, indicating the application of this proposed phagosensor in clinical settings as a rapid, alternative to available conventional detection techniques, and low-cost surveillance tool.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100238"},"PeriodicalIF":6.5,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000547/pdfft?md5=b366cf7e159c83256f01d5f19525cd30&pid=1-s2.0-S2666053924000547-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142163852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Engineers guide to Terfenol-D actuators: Design, performance, and real-world applications Terfenol-D 执行器工程师指南:设计、性能和实际应用
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-08-21 DOI: 10.1016/j.snr.2024.100236
Mukund A. Patil , Ravikiran Kadoli
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