Sensors and Actuators Reports最新文献

{"title":"Unleashing the potential of UiO-66-NH2 nanozyme: A novel approach to exosomal biomarkers detection in body fluids","authors":"Sara Soleimani-Porgou ,&nbsp;Tahereh Rohani Bastami ,&nbsp;Maryam M. Matin ,&nbsp;Azadeh Haghighitalab ,&nbsp;Hesamaddyn Babaei ,&nbsp;Saeedeh Pourhamedi ,&nbsp;Ahmad Reza Bahrami","doi":"10.1016/j.snr.2025.100329","DOIUrl":"10.1016/j.snr.2025.100329","url":null,"abstract":"<div><div>Exosomes, a subgroup of extracellular vesicles (EVs), are gaining their position as promising tools for diagnostic and treatment purposes. Their widespread application is however hindered by the lack of cost-effective and straightforward methods for detection and characterization. Addressing this challenge, our study introduces a colorimetric nanosensor utilizing a UiO-66-NH₂ nanozyme (UiO-66-NH₂ NZ) for the detection of exosomes. The nanozyme was synthesized successfully and characterized using various techniques including XRD, FTIR, EDS, TEM, DLS, and zeta potential. For this purpose, exosomes from human TERT-immortalized MSCs (hTERT-MSCs) were extracted and then characterized using FE-SEM imaging, AFM imaging, DLS analysis, zeta potential measurement, and flow cytometry for specific markers. The efficacy of UiO-66-NH₂ NZs was tested for conditioned cell culture medium and human blood plasma. In this way, the limits of detection (LOD) and the trend of response for different concentrations of exosomes were defined. Based on a colorimetric analysis, at lower exosome concentrations, the nanozyme induced a visible color shift from colorless to green-blue within two minutes. The selectivity of our method was validated by comparing exosome concentrations with those of exosome lysates and cell suspensions. Further assessment was conducted by confirming the suitability of the test for the samples from blood plasma, with potential applications at the clinical level. Compared with CD63 aptamer-modified nanozymes, the utility of bare UiO-66-NH₂ NZs in exosome detection was prominent. The rapid detection of exosomes from HT-29 colorectal adenocarcinoma cells and HFF fibroblastic cells confirmed the suitability of this assay as a promising approach for developing specific exosome-based clinical applications.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100329"},"PeriodicalIF":6.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and low-cost droplet pcr using a novel microfluidic setup with a numerical study on micromixer effects","authors":"Mohammad Hossein Pourghasemian ,&nbsp;Ali Kheirkhah Barzoki ,&nbsp;Mahdi Dizani ,&nbsp;Amir Hossein Kazemipour Ashkezari ,&nbsp;Amir Shamloo","doi":"10.1016/j.snr.2025.100327","DOIUrl":"10.1016/j.snr.2025.100327","url":null,"abstract":"<div><div>Droplet microfluidics, recognized for its precise dispensing and sample handling capabilities at microliter and lower volumes, has emerged as a popular method for miniaturizing polymerase chain reaction (PCR) platforms. This technology enables the accurate detection of low-abundance biological variations through droplet PCR (dPCR). In this study, we introduce a low-cost PCR thermal cycler setup, an optical system for DNA detection, and a 3D-printed droplet-based microfluidic device engineered to produce hundreds of nanoliter water-in-oil droplets. The microfluidic chip incorporates 21 cycles, each equipped with a micromixer. For the first time, we investigate the impact of integrating micromixers into the PCR chip cycles and demonstrate that this integration enhances the efficiency and specificity of the PCR process. Following the completion of the PCR cycles, the droplets are gathered in a chamber located at the end of the chip for amplification detection. A simple fluorescent optical setup is developed for fluorescence detection and accurate measurement of DNA concentration in the sample. This setup comprises a cost-effective high-resolution camera, a dichroic mirror, and an LED light source. Subsequently, the fluorescent images captured are processed using Python (OpenCV library) and analyzed for fluorescent emission intensity and droplet size distribution. The outcomes of the dPCR conducted with the microfluidic chip are subsequently assessed through a gel electrophoresis test and compared to the identical results from a commercial quantitative PCR (qPCR) device. Our study demonstrates the ability to perform rapid dPCR analysis via endpoint imaging utilizing a cost-effective and easy-to-fabricate setup.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100327"},"PeriodicalIF":6.5,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143870868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive gold nanostar based optical fiber sensor with tunable plasmonic resonance","authors":"Amin Moslemi ,&nbsp;Lucia Sansone ,&nbsp;Flavio Esposito ,&nbsp;Carlos Marques ,&nbsp;Stefania Campopiano ,&nbsp;Michele Giordano ,&nbsp;Agostino Iadicicco","doi":"10.1016/j.snr.2025.100326","DOIUrl":"10.1016/j.snr.2025.100326","url":null,"abstract":"<div><div>In this work, we present a detailed numerical and experimental investigation of highly sensitive optical fiber sensors based on localized surface plasmon resonance (LSPR). These sensors are enhanced by the deposition of nanoparticles (NPs) and nanostars (NSs) onto uncladded silica multi-mode optical fiber. The unique optical properties of NSs - featuring a 40 nm gold core surrounded by silver branches of variable size and shape - allows for precise tuning of the LSPR effect. For comparison, we also explored spherical gold NPs with a 40 nm diameter to assess performance differences. Our findings, both numerical and experimental, demonstrate that the LSPR wavelength and sensitivity to surrounding refractive index can be finely tuned by adjusting the morphology of the NS branches. This is achieved by varying the silver nitrate content during their synthesis. Using the Finite Element Method-based design tool we performed simplified study cases, that led to experimental sensitivity of approximately 560 nm/RIU for an LSPR wavelength near 810 nm. As a practical demonstration, the sensor was successfully employed to detect Thiram, a common agricultural pesticide, with a wide dynamic range from 10 pM to 100 µM and an impressive low limit of detection of 0.3 pM. Moreover, we investigated the sensor selectivity, stability and response to environmental temperature changes. This study emphasizes the simplicity, cost-effectiveness, and tunable performance of NS-based optical fiber sensors. By manipulating nanostructure morphology, we can significantly enhance sensor performance, positioning this technology as a highly promising solution for environmental monitoring, biomedical diagnostics, and chemical detection.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100326"},"PeriodicalIF":6.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of low-cost impedimetric biosensors for the quantitative detection of cortisol and the human growth hormone","authors":"Rachel Ashton ,&nbsp;Mohammad Rizwan ,&nbsp;Christopher Gwenin","doi":"10.1016/j.snr.2025.100325","DOIUrl":"10.1016/j.snr.2025.100325","url":null,"abstract":"<div><div>Cortisol and human growth hormone can be used as reliable biomarkers for the response of the hypothalamus-pituitary-adrenal axis. The hypothalamus-pituitary-adrenal axis is complex and dependent on many factors. However, generally, increased cortisol production is the hypothalamus-pituitary-adrenal axis's physiological response to an external stressor. Growth hormone is also regulated through the hypothalamus-pituitary-adrenal axis and secreted from the anterior pituitary gland. Hence point-of-care sensing devices for the quantification of cortisol and growth hormone would be versatile for the diagnosis of hypothalamus-pituitary-adrenal axis activity. The practical uses for a stress indicator would be diverse, inclusive of the medical and pharmaceutical sectors as well as sports and performance. Therefore, this work discusses the development of two electrochemical biosensors, designed to accurately detect and quantify cortisol and growth hormone concentration levels with potential practical applications. The device relies on impedance values which are produced when cortisol and growth hormone are immobilized onto electrode surfaces through antibody-antigen bonding. Calibration data was achieved for both Gold (Au, 111) slide electrodes and inexpensive screen-printed gold electrode-based sensor biochips with a strong correlation between impedance and concentration of cortisol, with detection range 30–300 ng/mL for Au (111) platform and 60 – 360 ng/mL for screen-printed gold electrode platform, while recombinant human growth hormone detection range was observed 5–30 ng/mL using both Au (111) platform, and screen-printed gold electrode platform.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100325"},"PeriodicalIF":6.5,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated microphotonic platform for highly sensitive virus detection","authors":"A. la Grasta ,&nbsp;M.I. Gómez-Gómez ,&nbsp;A. Griol ,&nbsp;E. Gómez ,&nbsp;M. De Carlo ,&nbsp;V.M.N. Passaro ,&nbsp;A. Martínez ,&nbsp;F. Dell’Olio","doi":"10.1016/j.snr.2025.100320","DOIUrl":"10.1016/j.snr.2025.100320","url":null,"abstract":"<div><div>Chip-scale micro-photonic biosensors have matured significantly over the past two decades, with various platforms developed for detecting biomarkers, including proteins in biological fluids. The COVID-19 pandemic has further accelerated research in virus detection, leading to the development of resonant and interferometric biosensors for identifying viruses like SARS-CoV-2. Despite these advancements, there remains a need for technical solutions that ensure point-of-care features such as low cost, rapid response, and low limit-of-detection (LoD). This paper presents an integrated silicon nitride microphotonic platform based on ring resonator technology, specifically designed for highly sensitive virus detection. The sensors, fabricated using strip and slot waveguides, are tested under flow conditions using ethanol and BSA-antiBSA solutions to evaluate bulk and surface sensitivity. The results indicate that the slot waveguide-based ring resonators exhibit the highest sensitivity. The platform uses functionalization protocols to immobilize specific antibodies for detecting SARS-CoV-2. The sensor successfully identifies the target analyte at concentrations as low as 1.5 nM, with a LoD of 36 pM using in situ functionalization, underscoring the importance of antibody orientation through an intermediate protein A layer. These results demonstrate the platform’s potential for rapid and sensitive virus diagnosis and represent a significant step toward the development of portable, point-of-care diagnostic technologies for infectious disease monitoring.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100320"},"PeriodicalIF":6.5,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-sensitive aptameric field-effect transistor for cortisol sensing","authors":"Thi Thanh Ha Nguyen ,&nbsp;Cong Minh Nguyen ,&nbsp;Minh Anh Huynh ,&nbsp;Quang Thang Trinh ,&nbsp;Philip Tanner ,&nbsp;Sven Ingebrandt ,&nbsp;Xuan Thang Vu ,&nbsp;Tuan-Khoa Nguyen ,&nbsp;Nam-Trung Nguyen","doi":"10.1016/j.snr.2025.100324","DOIUrl":"10.1016/j.snr.2025.100324","url":null,"abstract":"<div><div>Existing diagnostic methods for major depressive disorders, emerging mental disorders globally, are restrictive and inaccurate due to the lack of evidence-based assessments. Recent developments incorporating advanced material, micro/nano manufacturing and biology enable new clinical pathways using biosensors that can provide new opportunities for the diagnosis of mental condition from an early developmental stage. In this work, we present an ultra-sensitive lab-on-chip biosensor platform that is capable of detecting cortisol, a key biomarker for depression in physiological fluid. Utilizing advanced microfabrication techniques, our biosensor achieves a compact footprint of 5.0 mm by 2.5 mm, making it suitable for integration into portable and wearable devices. The device exhibits an ultra-low detection limit of 1 fM and a wide dynamic detection range spanning from 1 fM to 1 µM, covering the concentration present in physiological body fluids of human. Our finding shows a great potential for the development of accurate, and point-of-care monitoring system for early detection of depression disorders to advance the mental health diagnosis.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100324"},"PeriodicalIF":6.5,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Argonaute-based dual lateral flow test for SARS-CoV-2 and influenza a virus","authors":"Xingyu Ye ,&nbsp;Zhonglei Li ,&nbsp;Zhengwei Fang ,&nbsp;Nan Shen ,&nbsp;Yani Zhang ,&nbsp;Hanming Wang ,&nbsp;Yi Liu ,&nbsp;Yuanjie Zhou ,&nbsp;Shanshan Fan ,&nbsp;Peng Sun ,&nbsp;Yue Zhong ,&nbsp;Qian Liu ,&nbsp;Yan Feng","doi":"10.1016/j.snr.2025.100321","DOIUrl":"10.1016/j.snr.2025.100321","url":null,"abstract":"<div><div>The lateral flow test (LFT) is crucial for community screening and early warning of infectious pathogens due to its portability and simplicity. However, antigen-based LFTs have relatively low sensitivity, while nucleic acid-based LFTs are limited in specificity and multiplexing capabilities, thus requiring improvements in accuracy and efficiency. In this study, we developed a dual lateral flow assay utilizing a thermophilic Argonaute, enabling the detection of multiple nucleic acids on a single strip with a sensitivity of 3 copies/μL and high specificity. Clinical validation showed 100 % concordance with commercial RT-qPCR results. This method not only preserves the high sensitivity of nucleic acid detection but also provides the benefits of portability, dual-target detection, and visual readout, highlighting its potential for point-of-care applications.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100321"},"PeriodicalIF":6.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boundary engineering of porous zinc oxide nanosheets to enhance gas sensing performance","authors":"Min Young Kim ,&nbsp;Haeun Choa ,&nbsp;Chul Oh Park ,&nbsp;Dung Thi Hanh To ,&nbsp;Tran Khanh Phuong Cao ,&nbsp;Emily Resendiz Mendoza ,&nbsp;Changhyun Jin ,&nbsp;Beom Zoo Lee ,&nbsp;Nosang Vincent Myung ,&nbsp;Kyu Hyoung Lee","doi":"10.1016/j.snr.2025.100322","DOIUrl":"10.1016/j.snr.2025.100322","url":null,"abstract":"<div><div>Zinc oxide (ZnO) based gas sensors are noted for their high sensing response toward a wide range of gases, however, they often face challenges in achieving other required properties due to complex reaction dynamics under different operating conditions. Herein, we fabricated boundary-engineered porous ZnO nanosheets by controlling calcination temperature of hydrozincite. These materials were systematically evaluated their sensing performance towards oxidizing gases, reducing inorganic gases, and volatile organic compounds. Additionally, detailed sensing properties toward NO₂, NH₃, and acetone were examined to investigate the critical roles of the energy barrier for carrier transport (<em>E</em>_b) and the nature of ionized oxygen species in the sensing materials. Characteristics of the materials, such as crystallite size, pore size, porosity, and surface area, were effectively tuned, which significantly influenced <em>E</em>_b and sensing performance. The porous ZnO nanosheets calcined at 300 °C for 4 h, exhibiting a higher density of boundaries, demonstrated enhanced sensing performance with maximum response values of 10.59 for NO₂, 0.38 for NH₃, and 0.41 for acetone at personnel exposure level. This improvement is likely linked to the increased surface charge carrier concentration and the higher <em>E</em>_b associated with greater boundary density, indicating an increase in available reaction active sites. Furthermore, our findings reveal that gas sensing behavior is strongly dependent on the type of ionized oxygen species present. The optimal sensing temperature for NO₂ is 250 °C, where the dominant ionized oxygen species is <em>O</em>⁻, while for NH₃ and acetone, the optimal temperature was 450 °C, where O²⁻ is the predominant species.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100322"},"PeriodicalIF":6.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile patterning of microfluidic paper-based analytical devices (μPADs) by dispensing propylene glycol methyl ether acetate (PGMEA)","authors":"Xionghui Li ,&nbsp;Xuanying Liang ,&nbsp;Haonan Li ,&nbsp;Jing Song ,&nbsp;Kanghui Li ,&nbsp;Muyang Zhang ,&nbsp;Huiru Zhang ,&nbsp;Zhuoting Han ,&nbsp;Lok Ting Chu ,&nbsp;Weijin Guo","doi":"10.1016/j.snr.2025.100323","DOIUrl":"10.1016/j.snr.2025.100323","url":null,"abstract":"<div><div>Facile patterning of microfluidic paper-based analytical devices (μPADs) is highly intriguing for researchers using μPADs for point-of-care diagnostics. For the first time, this work introduces the use of propylene glycol methyl ether acetate (PGMEA) as a novel material to create barriers on μPADs, with the formation mechanism of these barriers investigated using scanning electron microscopy (SEM). A digitized and automated dispensing machine is employed for barrier creation, demonstrating the stability of the barriers in the presence of various organic and aqueous solvents including alkaline and acidic solutions, and surfactants. Additionally, we explore the possibility of rapid patterning of μPADs by hand drawing using PGMEA. The stability of the barriers created by PGMEA lays a solid foundation for various biomedical and chemical analyses. Moreover, we use this method to prepare μPADs for quantitative measurement of iodate content in saline solutions and blood typing analysis successfully, proving that μPADs prepared by this method have a significant potential for applications in point-of-care diagnostics.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100323"},"PeriodicalIF":6.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Meander-shaped piezoelectric MEMS loudspeaker with maximized area efficiency for in-ear applications","authors":"Dennis Becker ,&nbsp;Robert Scharf ,&nbsp;Tobias Leonhard ,&nbsp;Andreas Merz ,&nbsp;Achim Bittner ,&nbsp;Alfons Dehé","doi":"10.1016/j.snr.2025.100319","DOIUrl":"10.1016/j.snr.2025.100319","url":null,"abstract":"<div><div>State-of-the-art MEMS loudspeakers use innovative actuator concepts to improve the acoustic performance from minimal chip volumes. Lateral vibration of multiple vertical actuators is a promising lever to increase the active emitting area while keeping the chip dimensions constant. Recent publications on this topic require a multiple wafer fabrication process to realize such actuator principles. This work demonstrates a new concept fabricating a closed folded membrane with vertical actuators in a single wafer process. Conformal deposition of piezoelectric aluminum nitride (AlN) thin films allow the lateral actuation of the transducer. Laser-doppler-vibrometer (LDV) measurements show that no actuator resonances are located below 40 kHz. Acoustic characterization of a manufactured device in an IEC ear simulator demonstrates a linear behavior of this loudspeaker with THD values below 1.5 % over the audible frequency range. At 1 kHz, a SPL of 64 dB and a THD of 0.3 % are measured with a driving voltage of 16 V_p. A comparison of the acquired frequency response with simulated results shows a very good fit with deviations of less than 3.2 dB, successfully proving the concept and the potential of the folded MEMS loudspeaker.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100319"},"PeriodicalIF":6.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}