Sensors and Actuators Reports最新文献

{"title":"Detection of HPV-16 L1 antigen using MoS2 nanosheets and amine-functionalized copper doped carbon dots","authors":"Rajmohan Swetha ,&nbsp;Karutha Pandian Divya ,&nbsp;Lakshmi Devi A ,&nbsp;T.G. Satheesh Babu","doi":"10.1016/j.snr.2025.100308","DOIUrl":"10.1016/j.snr.2025.100308","url":null,"abstract":"<div><div>HPV-16L1 is one of the important biomarkers for chronic cervical cancer. The present work deals with the development of a label-free electrochemical immunosensor by modification of electrode surface using 2D MoS₂ nanosheets and amine functionalized copper doped carbon dots (f-CuCD). The copper doped carbon dot with high surface area 2D material like MoS₂ provides a stable platform for the immobilization of antibody which aids in the sensor performance. The use of MoS₂/f-CuCD is the highlight of this work through which effective anti-HPV immobilization on the surface is achieved. Electrochemical impedance spectroscopy (EIS) was used to analyse the response of different concentrations of HPV-16L1 on the modified screen printed carbon electrode (SPCE). On increasing the concentration of HPV-16L1, the R_CT value gradually increased and the peak current decreased due to the low conductivity of the protein molecule. The developed immunosensor was found to detect wide concentration ranges of 0.01–100 ng/µL with a low detection limit of 8.30 pg/µL for HPV-16L1. Spiked human serum samples were also analysed. It also proves to be a sensitive, selective, stable and feasible tool for the determination of HPV-16L1.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100308"},"PeriodicalIF":6.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multiplexed laser-scribed graphene immunosensor for simultaneous detection of adiponectin and leptin: A point-of-care solution for early insulin resistance diagnosis","authors":"Lavita Nuraviana Rizalputri ,&nbsp;José Ilton de Oliveira Filho ,&nbsp;Saptami Suresh Shetty ,&nbsp;Eckaard le Roux ,&nbsp;Abdullah Bukhamsin ,&nbsp;Bandar Alshehri ,&nbsp;Veerappan Mani ,&nbsp;Khaled Nabil Salama","doi":"10.1016/j.snr.2025.100306","DOIUrl":"10.1016/j.snr.2025.100306","url":null,"abstract":"<div><div>The increasing prevalence of insulin resistance (IR) highlights the importance of early diagnosis and timely medical intervention. The ratio of adiponectin to leptin proteins (A/L) is believed to be a key indicator of IR, but the lack of adequate ways of tracking it prevents its clinical utilization. To that end, we have developed a laser-scribed graphene (LSG) based immunosensor capable of detecting adiponectin and leptin simultaneously. The sensors are decorated with gold nanostructure (AuNS) that are bio-functionalized with adiponectin and leptin antibodies via EDC/NHS covalent chemistry. We demonstrate that the immunosensor is suitable for clinical utilization, with a detection limit of 0.59 μg/mL for adiponectin and 0.18 ng/mL for leptin. Furthermore, the multiplexed nature of the assay allows for IR assessments at reduced sample volume whilst not compromising accuracy. Lastly, we validate the applicability of the immunosensor for point-of-care (PoC) settings by integrating with a customized miniaturized potentiostat connected to a smartphone application. The resulting device can be used for the facile quantification of A/L at clinically relevant accuracy, facilitating faster IR assessment relative to conventional methods that rely on independent measurements of the two biomarkers.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100306"},"PeriodicalIF":6.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode ratiometric electrochemical and turn-on fluorescent probe for reliably detecting H2O2 in Parkinson's disease serum","authors":"Hui Dong ,&nbsp;Linlin Zheng ,&nbsp;Zuying Wang ,&nbsp;Ke Xu ,&nbsp;Wentian Chen ,&nbsp;Zi Liu ,&nbsp;Zhu Chang ,&nbsp;Yanli Zhou ,&nbsp;Xu Zhu ,&nbsp;Yintang Zhang ,&nbsp;Maotian Xu","doi":"10.1016/j.snr.2025.100305","DOIUrl":"10.1016/j.snr.2025.100305","url":null,"abstract":"<div><div>Accurate diagnosis and effective treatment of Parkinson's disease (PD) rely on the accurate detection of the corresponding biomarkers. The build-up of reactive oxygen species (ROS), for instance, hydrogen peroxide (H₂O₂), resulting in oxidative stress inside cells is closely related to the pathogenesis of PD. In this study, two compounds, Re-B and Re-OB, were synthesized through a simple one-step reaction. Upon reacting with H₂O₂, the borate ester group in Re-B is selectively cleaved, restoring the phenol group. This leads to both a dual-signal ratiometric electrochemical response and a ``turn-on'' fluorescence effect. The Re-B probe demonstrates a clear ``turn-on'' electrochemical current response at −311.1 mV, followed by a ``turn-off'' response at −257.6 mV when interacting with H<sub>2</sub>O<sub>2</sub> on a single-walled carbon nanotube-modified screen-printed carbon electrode (SPCE/SWCNTs). Concurrently, the fluorescence spectrum shows a ``turn-on'' response with an emission peak at 586 nm. The fluorescence and ratiometric electrochemical methods developed in this study show exceptionally high sensitivity and selectivity for H₂O₂, with detection limits as low as 0.5 μM for the electrochemical method and 0.1 μM for fluorescence. The consistency between the results from both electrochemical and fluorescence quantification of H₂O₂ levels in serum underscores its reliability. This approach has significant potential as a point-of-care diagnostic device for PD early detection, providing valuable information for timely diagnosis and treatment.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100305"},"PeriodicalIF":6.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dielectrophoresis-based microfluidics for detection and separation of circulating tumor cells","authors":"Iman Najafipour ,&nbsp;Pegah Sadeh ,&nbsp;Ali Mohammad Amani ,&nbsp;Hesam Kamyab ,&nbsp;Shreeshivadasan Chelliapan ,&nbsp;Saravanan Rajendran ,&nbsp;Ana Belén Peñaherrera-Pazmiño ,&nbsp;Sajad Jamalpour","doi":"10.1016/j.snr.2025.100304","DOIUrl":"10.1016/j.snr.2025.100304","url":null,"abstract":"<div><div>Circulating tumor cells (CTCs) represent a critical focus in cancer research due to their potential to enable early detection, monitor disease progression, and facilitate personalized therapies. However, existing isolation techniques often face significant limitations, including low specificity, reduced recovery rates, and the inability to preserve cellular viability for downstream applications such as genetic profiling and drug testing. This review addresses a key knowledge gap in the development of efficient, label-free, and scalable technologies for CTC isolation, emphasizing the role of dielectrophoresis (DEP)-based microfluidic systems. DEP leverages the intrinsic dielectric properties of cells to enable selective and non-invasive separation, eliminating the need for surface markers and ensuring high cell integrity. The study highlights the integration of nanomaterials, such as gold nanoparticles and graphene oxide nanosheets, as a novel approach to overcome existing challenges in DEP-based platforms. These nanomaterials improve the specificity and sensitivity of CTC capture by increasing surface area and biocompatibility. Key advancements discussed include the optimization of electrode designs, tuning of electric field parameters, and innovative system configurations that enhance recovery efficiency and separation purity. The review also compares various DEP configurations, such as electrode-based, insulator-based, and contactless systems, evaluating their unique advantages and suitability for different applications. In addition to reviewing current advancements, the paper outlines future directions for the field, emphasizing the need for large-scale clinical validation to establish DEP-based systems as reliable diagnostic tools. This review provides a comprehensive framework for advancing DEP-based microfluidic platforms, offering a transformative approach for early cancer detection, personalized medicine, and the broader application of innovative diagnostic technologies in clinical settings.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100304"},"PeriodicalIF":6.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ratiometric fluorescent biosensors for quantitative lactic acid detection using CdTe@CdS quantum dots and lactate oxidase","authors":"Giuliana Grasso ,&nbsp;Riccardo Scarfiello ,&nbsp;Francesco Colella ,&nbsp;Valentina Onesto ,&nbsp;Stefania Forciniti ,&nbsp;Giuseppe Gigli ,&nbsp;Luigi Carbone ,&nbsp;Loretta L. del Mercato","doi":"10.1016/j.snr.2025.100303","DOIUrl":"10.1016/j.snr.2025.100303","url":null,"abstract":"<div><div>Lactic acid plays a crucial role in various physiological processes, particularly in cellular metabolism and muscle activity, as it is a key marker of anaerobic respiration and metabolic stress. In this study, we present a ratiometric fluorescent biosensor for lactic acid detection, utilizing a fluorescence quenching mechanism. The sensor comprises a hydrogen peroxide (H₂O₂)-sensing unit, based on photoluminescent core-shell cadmium telluride@cadmium sulfide quantum dots (CdTe@CdS QDs), along with a reference probe covalently bound to silica microparticle (SiO₂ MPs), which acts as the substrate. Lactate oxidase (LOx) is immobilized on the microparticle surface, where it catalyzes the aerobic oxidation of <span>l</span>-lactate into pyruvate, generating H₂O₂ in the process. The increasing concentrations of lactate (0–30 mM) result in proportional quenching of the CdTe@CdS QDs’ photoluminescence due to H₂O₂, while the reference fluorescence emission remains stable. A lactate calibration curve has been determined using confocal laser scanning microscopy (CLSM), enabling quantitative evaluation of the sensor's ratiometric response and colorimetric shifts via image analysis. To demonstrate the versatility of this approach, we engineered two variations of the microsensor with distinct ratiometric setups, one using green- and the other red-emitting CdTe@CdS QDs, paired with either rhodamine isothiocyanate (RBITC) or 7-(diethylamino)coumarin-3-carboxylic acid (7ACC1) as the reference dyes, both co-immobilized with LOx enzyme on the microparticle surface. These innovative microsensors offer a simple yet effective tool for the quantitative detection of lactic acid, leveraging its H₂O₂-sensing capability of CdTe@CdS QDs in combination with to LOx enzyme activity.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100303"},"PeriodicalIF":6.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applications of piezoelectric-based sensors, actuators, and energy harvesters","authors":"Mohammad Ali Mangi ,&nbsp;Hassan Elahi ,&nbsp;Ahsan Ali ,&nbsp;Hamid Jabbar ,&nbsp;Anas Bin Aqeel ,&nbsp;Aasia Farrukh ,&nbsp;Saira Bibi ,&nbsp;Wael A. Altabey ,&nbsp;Sallam A. Kouritem ,&nbsp;Mohammad Noori","doi":"10.1016/j.snr.2025.100302","DOIUrl":"10.1016/j.snr.2025.100302","url":null,"abstract":"<div><div>From the last few decades, piezoelectric materials have played an important role in the field of sensing and actuating. They have voltage-dependent actuation, which allows them to serve as energy harvesters and structural health monitoring of the system. This phenomenon makes piezoelectric sensors and actuators useful for monitoring and controlling various physical phenomena, such as vibration, shock, position, displacement, stress, strain load, and precision motion. In this review article, a comprehensive summary of the uses of piezoelectric sensors, actuators and energy harvesters is elaborated in detail. Moreover, the discussion on the different types of piezoelectric sensors, actuators and energy harvesters, their working principles, different configurations and their use in various industrial applications is carried out. Overall, this review article demonstrates the versatility and effectiveness of piezoelectric sensors and actuators in various industrial applications and provides valuable insights for researchers, engineers, and industry professionals.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100302"},"PeriodicalIF":6.5,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143508662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative ferrite sensors: Advancing non-enzymatic glucose detection","authors":"Rohit Jasrotia ,&nbsp;Kanika Raj ,&nbsp;Basant Singh ,&nbsp;Abdullah H. Alluhayb ,&nbsp;Alaa M. Younis ,&nbsp;Suman ,&nbsp;M. Ramya ,&nbsp;Abhishek Kandwal ,&nbsp;Virat Khanna ,&nbsp;Virender Pratap Singh ,&nbsp;Chan Choon Kit","doi":"10.1016/j.snr.2025.100295","DOIUrl":"10.1016/j.snr.2025.100295","url":null,"abstract":"<div><div>Ferrite-based nanomaterials have surfaced as capable candidates for glucose sensing owing to their distinctive magnetic, electrical, and structural properties. This review article offers a wide-ranging overview of current studies in the expansion and use of ferrite-based glucose sensors. In this review, the synthesis methods and characterization techniques for the various ferrite nanomaterials, including spinel ferrite, highlighting their enhanced sensitivity, stability, and selectivity in glucose detection has been discussed thoroughly. The mechanism of glucose sensing is explained, focusing on the role of ferrite nanoparticles in facilitating electron transfer and catalytic activity. Additionally, the integration of ferrite-based sensors with the various transducing elements such as electrochemical, optical, and magnetic systems is examined. Alongside, obstacles and future scopes in this field has been explained, emphasizing the potential for ferrite-based materials to aid to the enhancement of low-cost, and non-invasive glucose monitoring devices with high efficiency. Through this critical analysis, we aim to provide insights into the ongoing research and inspire future innovations in ferrite-based glucose sensing technologies.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100295"},"PeriodicalIF":6.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of molecular structure and plasticization of PVC membranes in the response of solid-state ion-selective electrodes","authors":"Coral Salvo-Comino ,&nbsp;Luis E. Alonso-Pastor ,&nbsp;Clara Pérez-González ,&nbsp;Stefano Pettinelli ,&nbsp;Karina C. Núñez Carrero ,&nbsp;Miguel Ángel Rodríguez-Pérez ,&nbsp;María Luz Rodríguez-Méndez","doi":"10.1016/j.snr.2025.100301","DOIUrl":"10.1016/j.snr.2025.100301","url":null,"abstract":"<div><div>Potentiometric sensors are essential in various industries due to their high sensitivity, simplicity, and cost-effectiveness. However, enhancing sensitivity, largely dependent on membrane diffusion, remains challenging. This study investigates the role of polyvinyl chloride (PVC) molecular weight and plasticizer type on optimizing potentiometric sensor performance for tartaric acid detection. Two PVCs with different molecular weights (HIGH and LOW) and distinct plasticizers were evaluated. Results demonstrated that membranes plasticized with dibutyl sebacate, a linear plasticizer, exhibited high sensitivity (0.0239 mV/pX) and repeatability, achieving a lower limit of detection (LOD = 4.31 × 10⁻⁵ M) compared to those with a bulky plasticizer. Specifically, HIGH PVC combined with a linear plasticizer provided saturation-free measurements, ensuring greater accuracy in tartaric acid quantification. This research underscores the impact of plasticizer selection on sensor optimization, enhancing accuracy and reliability in food quality control. By establishing the relationship between PVC structure and plasticizer type, this study contributes to advancing sensor technology for improved food safety and quality assessment.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100301"},"PeriodicalIF":6.5,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted polypyrrole-modified screen-printed electrode: Toward a sensor for furfural derivatives determination","authors":"Daniele Merli ,&nbsp;Alessandra Cutaia ,&nbsp;Sofia Prosperi ,&nbsp;Camilla Zanoni ,&nbsp;Giancarla Alberti","doi":"10.1016/j.snr.2025.100299","DOIUrl":"10.1016/j.snr.2025.100299","url":null,"abstract":"<div><div>The increasing interest in determining furfural derivatives in food and beverages using low-cost and portable devices has driven our attention to the development of the here proposed voltammetric sensor. It is based on a screen-printed electrochemical cell whose graphite-ink working electrode is surface modified with electropolymerized molecularly imprinted polypyrrole (e-MIP), using 5-(hydroxymethyl)furan-2-carbaldehyde (HMF) as the template. Square wave voltammetry (SWV) is the selected analytical technique, exploiting the irreversible reduction peak of the carbonyl group at -1.7 V vs. Ag/AgCl pseudo reference electrode in 0.1 M phosphate buffer (PBS) at pH 8. The presence of the e-MIP on the working electrode surface enhances the sensitivity by lowering the detection limit of about one order of magnitude if compared with the bare electrode (LOD bare 93 µM; e-MIP 7 µM). The developed sensor is selective for the whole class of furfural-like compounds, allowing the determination of the total furanic aldehydes content. The sensor was applied to the analysis of synthetic solutions and real beverages, such as expresso coffee, obtaining good recovery percentages.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100299"},"PeriodicalIF":6.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143334063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acoustic bubbles: Inducing cell interactions via trapping, patterning, and removal","authors":"Jonathan Faulkner ,&nbsp;Mengren Wu ,&nbsp;Madelyn Wicker ,&nbsp;Yuan Gao","doi":"10.1016/j.snr.2025.100294","DOIUrl":"10.1016/j.snr.2025.100294","url":null,"abstract":"<div><div>Studying cell-cell interactions is crucial for understanding biological processes and advancing drug development. Recent advancements in microfluidic technology allow cell-cell interaction experiments to be performed on-chip, enabling the study of specific interactions within a highly defined microenvironment. Acoustic bubbles, tiny bubbles excited by an external acoustic source, are clean, biocompatible, and effective tools for manipulating fluids and particles at the microscale. This research presents a novel microfluidic approach using acoustic bubbles to study cell-cell interactions. It focuses on generating, controlling, and removing acoustic bubbles within a microfluidic device to manipulate particles and cells and induce their interaction. In this work, a microbubble is passively generated to separate the channels containing two different types of particles and cells. Under the control of an acoustic field, particles and cells are trapped on either side of the microbubble. The microbubble is then removed to enable interaction between the trapped particles and cells. The impact of channel geometry on bubble stability is quantitatively investigated to optimize the device's performance. Additionally, the successful demonstration of on-chip cell interactions highlights the rapid and straightforward operation of this method. This innovative approach holds great potential for studying specific cell interactions to gain biological insights into disease progression and serves as an effective tool for drug testing.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100294"},"PeriodicalIF":6.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}