Argonaute-based dual lateral flow test for SARS-CoV-2 and influenza a virus

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports Pub Date : 2025-04-02 DOI:10.1016/j.snr.2025.100321

Xingyu Ye , Zhonglei Li , Zhengwei Fang , Nan Shen , Yani Zhang , Hanming Wang , Yi Liu , Yuanjie Zhou , Shanshan Fan , Peng Sun , Yue Zhong , Qian Liu , Yan Feng

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引用次数: 0

Abstract

The lateral flow test (LFT) is crucial for community screening and early warning of infectious pathogens due to its portability and simplicity. However, antigen-based LFTs have relatively low sensitivity, while nucleic acid-based LFTs are limited in specificity and multiplexing capabilities, thus requiring improvements in accuracy and efficiency. In this study, we developed a dual lateral flow assay utilizing a thermophilic Argonaute, enabling the detection of multiple nucleic acids on a single strip with a sensitivity of 3 copies/μL and high specificity. Clinical validation showed 100 % concordance with commercial RT-qPCR results. This method not only preserves the high sensitivity of nucleic acid detection but also provides the benefits of portability, dual-target detection, and visual readout, highlighting its potential for point-of-care applications.

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基于 Argonaute 的 SARS-CoV-2 和甲型流感病毒双侧流试验

侧流试验（LFT）因其便携性和简便性，对社区筛查和传染病病原体预警至关重要。然而，基于抗原的侧流检测灵敏度相对较低，而基于核酸的侧流检测在特异性和复用能力方面受到限制，因此需要提高准确性和效率。在本研究中，我们利用嗜热 Argonaute 开发了一种双侧流检测法，可在单条试纸条上检测多种核酸，灵敏度为 3 个拷贝/μL，特异性高。临床验证表明，该方法与商业 RT-qPCR 结果的一致性达到 100%。这种方法不仅保持了核酸检测的高灵敏度，还具有便携、双目标检测和可视读数等优点，凸显了其在护理点应用方面的潜力。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.