Sensors and Actuators Reports最新文献_第3页

Highly efficient and toxicologically safe ZnO(NP)@GO nanocomposite in the simultaneous detection of Cd²⁺, Hg²⁺, Pb²⁺, and Zn²⁺ in aqueous medium 高效、毒理学安全的ZnO(NP)@GO纳米复合材料在水介质中同时检测Cd 2 +、Hg 2 +、Pb 2 +和Zn 2 +

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-08-11 DOI: 10.1016/j.snr.2025.100373

Melody Lalhruaitluangi , Lalruatkima Ralte , Diwakar Tiwari , Jinho Jung

{"title":"Highly efficient and toxicologically safe ZnO(NP)@GO nanocomposite in the simultaneous detection of Cd²⁺, Hg²⁺, Pb²⁺, and Zn²⁺ in aqueous medium","authors":"Melody Lalhruaitluangi , Lalruatkima Ralte , Diwakar Tiwari , Jinho Jung","doi":"10.1016/j.snr.2025.100373","DOIUrl":"10.1016/j.snr.2025.100373","url":null,"abstract":"<div><div>Miniaturised devices for trace, simultaneous and off-site detection are the need of the hour for protecting public health and the environment at large. Using sustainable and greener approach, the current study explores using <em>Acmella oleracea</em> extract to derive zinc oxide nanoparticles. Further, ZnO incorporates graphene oxide to obtain the nanocomposite material (ZnO(NP)@GO). The thin film electrode using the ZnO(NP)@GO efficiently detects potential heavy metal ions <em>viz.</em>, Cd²⁺, Hg²⁺, Pb²⁺, and Zn²⁺ individually and simultaneously at trace level concentrations. The thin film electrode using the ZnO(NP)@GO showed significantly enhanced electroactive surface area and reduced electron transfer resistance compared to the unmodified glassy carbon electrode. The calibration curve for detecting Cd²⁺, Hg²⁺, Pb²⁺, and Zn²⁺ shows the limits of detection of 0.026, 0.03, 0.010 and 0.020 µgL⁻¹, respectively, in their simultaneous detection. The ZnO(NP)@GO thin film electrode possesses good shelf life and stability for repeated detections of these heavy metal toxic ions. Furthermore, the thin film electrode detects simultaneously these ions at trace levels in the hospital effluent waters with real implications. Moreover, the acute toxicity of ZnO(NP)@GO towards <em>D. magna</em> shows an EC<sub>50</sub> value of 115.7 mgL⁻¹ after 48 hrs, proving the safety and environmental sustainability of the modified electrode.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100373"},"PeriodicalIF":7.6,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144860805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acid-resistant PVP/gold nanoparticles cluster for gastric juice-based metabolomics analysis 耐酸PVP/金纳米粒子簇用于胃液代谢组学分析

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-08-09 DOI: 10.1016/j.snr.2025.100370

Taeyeon Kim , Minsu Jang , Yeongjun Jeon , Seungwook Han , You Hwan Kim , Sunwoo Park , Hyeyun Lee , Woosok Moon , Tae-Young Jeong , Cheol Woong Choi , Jin-Woo Oh

{"title":"Acid-resistant PVP/gold nanoparticles cluster for gastric juice-based metabolomics analysis","authors":"Taeyeon Kim , Minsu Jang , Yeongjun Jeon , Seungwook Han , You Hwan Kim , Sunwoo Park , Hyeyun Lee , Woosok Moon , Tae-Young Jeong , Cheol Woong Choi , Jin-Woo Oh","doi":"10.1016/j.snr.2025.100370","DOIUrl":"10.1016/j.snr.2025.100370","url":null,"abstract":"<div><div>This study aims to develop a surface-enhanced Raman scattering (SERS)-based technology capable of directly analyzing metabolites in gastric juice using acid-resistant nanostructure for the diagnosis of gastric cancer. Although gastric cancer has a high incidence and mortality rate, existing endoscopic examination methods have limitations such as low sensitivity and operator dependency. In this study, we fabricated nanoparticles (NPs) cluster structure using Au NPs encapsulated in polyvinylpyrrolidone (PVP) to maintain structural stability even in strongly acidic environments. The PVP-Au NPs solution mixed with gastric juice was coated using the meniscus guided deposition technique to fabricate a uniform NPs cluster, thereby ensuring high chemical stability and reproducibility of SERS signals. The acquired SERS spectra were preprocessed and transformed into barcode-format features, which were then used to train a neural-network-based machine learning classification model. In the analysis of 121 gastric juice samples, the model accurately classified gastric cancer with a sensitivity of 91.7 % and a specificity of 91.7 % in the test set. This study demonstrates the potential of a high-precision gastric cancer diagnostic platform that integrates an acid-resistant PVP-based Au NPs cluster structure with machine learning classification and suggests its possible expansion as an auxiliary diagnostic tool for screening high-risk groups and predicting future prognosis.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100370"},"PeriodicalIF":7.6,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanically exfoliated MoTe2 thin film Photodetector with an ultra-broadband spectral response from ultraviolet to short-wavelength infrared 机械剥离MoTe2薄膜光电探测器，具有从紫外到短波红外的超宽带光谱响应

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-08-07 DOI: 10.1016/j.snr.2025.100372

Feng Zhou , Siyuan Yu , Xuanqi Zhong , Haiting Zhang , Xiaoxian Song

引用次数: 0

Recent process in organic near-infrared fluorescent probes for detecting hypochlorous acid/hypochlorite 有机近红外荧光探针检测次氯酸/次氯酸盐的最新进展

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-08-05 DOI: 10.1016/j.snr.2025.100371

Zhongchang Wang , Chenzheng Guan , Wenxu Lu , Liangchao Yuan , Man Chao , Hai-Liang Zhu , Tingwu Liu

{"title":"Recent process in organic near-infrared fluorescent probes for detecting hypochlorous acid/hypochlorite","authors":"Zhongchang Wang , Chenzheng Guan , Wenxu Lu , Liangchao Yuan , Man Chao , Hai-Liang Zhu , Tingwu Liu","doi":"10.1016/j.snr.2025.100371","DOIUrl":"10.1016/j.snr.2025.100371","url":null,"abstract":"<div><div>Hypochlorous acid (HClO) plays an essential role in physiological and pathological processes, Dysregulated HClO production has been implicated in various disease states. Consequently, <em>in vivo</em> HClO monitoring is crucial for elucidating disease pathogenesis. Among various detection strategies, near-infrared (NIR) fluorescent probes offer unparalleled advantages for bio-imaging. The design of NIR-HClO fluorescent probes has garnered increasing research focus. This review provides a critical evaluation of the latest advances (2021–2025) in the design and construction of organic NIR fluorescent probes for selectively monitoring HClO. We systematically emphasize four major recognition strategies based on the oxidation of carbon-carbon unsaturated bonds, chalcogenides, nitrogen-containing groups, and phenol analogues. Furthermore, we offer a comprehensive discussion on the overarching challenges in the field, including the delicate interplay between fluorophore stability and recognition moiety reactivity, the trend towards multifunctional and theranostic probes, and the formidable quest for activatable probes in the second near-infrared (NIR-II) window. Finally, we outline future prospects, aiming to inspire innovative designs that will overcome current bottlenecks and propel the field towards clinical translation. We believe that the continued development of advanced NIR fluorescent probes remains a prominent and vital trend for future research in chemical biology and diagnostics.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100371"},"PeriodicalIF":7.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144772998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polymer brushes for sensing in food systems: Opportunities and challenges span from organophosphorus pesticide to pathogen detection 用于食品系统传感的聚合物刷：从有机磷农药到病原体检测的机遇和挑战

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-07-30 DOI: 10.1016/j.snr.2025.100368

Raphael D. Ayivi , Bukola O. Adesanmi , Maria J. Torres , Sherine O. Obare , Carmen L. Gomes , Eric S. McLamore

{"title":"Polymer brushes for sensing in food systems: Opportunities and challenges span from organophosphorus pesticide to pathogen detection","authors":"Raphael D. Ayivi , Bukola O. Adesanmi , Maria J. Torres , Sherine O. Obare , Carmen L. Gomes , Eric S. McLamore","doi":"10.1016/j.snr.2025.100368","DOIUrl":"10.1016/j.snr.2025.100368","url":null,"abstract":"<div><div>Polymer brushes are tethered nanomaterial surface coatings that have important applications in smart release systems, pollutant degradation technologies, and sensors. Most applications of brushes have been in the medical domain, but there is an exciting opportunity to develop responsive interfaces for sensor applications in food systems. In this review article, we summarize common synthesis approaches as well as general mechanisms for stimulus-response behavior. We discuss emerging opportunities and challenges for polymer brush sensors in detection of six key targets in the food system: VOCs, nutraceuticals and bio-active compounds, pesticides, inorganic phosphates and other food additives, proteins such as allergens, and pathogens. Specific to food systems, major challenges include sample (matrix) complexity, material sustainability for sensor development, scalability of polymer brush synthesis, regulatory approval, and trust/transparency in new automated decision support approaches. Here, we also highlight general opportunities and challenges in polymer brush sensing related to advancing cyber-physical systems via decision support systems based on AI. Rapid sensing is crucial for monitoring systems in the farm-to-fork continuum. Polymer brushes are poised to play a key role in development of advanced interfacial control systems in smart sensor systems, opening new doors toward real time monitoring.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100368"},"PeriodicalIF":7.6,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PNA-SERS biosensor for label-free detection of SARS-CoV-2 genomic sequences in saliva 用于唾液中SARS-CoV-2基因组序列无标记检测的PNA-SERS生物传感器

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-07-28 DOI: 10.1016/j.snr.2025.100369

Alessandro Esposito , Sara Martino , Deniz Yilmaz , Maria Mangini , Luca De Stefano , Annunziata Corteggio , Paola Italiani , lIaria Rea , Anna Chiara De Luca

{"title":"PNA-SERS biosensor for label-free detection of SARS-CoV-2 genomic sequences in saliva","authors":"Alessandro Esposito , Sara Martino , Deniz Yilmaz , Maria Mangini , Luca De Stefano , Annunziata Corteggio , Paola Italiani , lIaria Rea , Anna Chiara De Luca","doi":"10.1016/j.snr.2025.100369","DOIUrl":"10.1016/j.snr.2025.100369","url":null,"abstract":"<div><div>The COVID-19 pandemic has emphasized the need for rapid, sensitive, and accessible molecular diagnostics. In this study, we present a label-free Surface-Enhanced Raman Spectroscopy (SERS) biosensor for the direct detection of SARS-CoV-2 RNA in biological fluids. The proposed sensor is based on a thiolated Peptide Nucleic Acid (PNA) probe immobilized on colloidal gold nanoparticles (AuNPs) deposited on functionalized glass substrates. A stable and selective hybridization with target sequences is provided by the intrinsic characteristics of PNA molecules, such as neutral backbone, high sequence affinity and enzymatic resistance. Whereas AuNPs enables strong signal enhancement and excellent reproducibility, without requiring complex nanofabrication techniques. Overall, the biosensor fabrication relies entirely on standard laboratory procedures and commercially available reagents, making it cost-effective and easily scalable. The detection of the target RNA occurs through label-free SERS, responsible for amplifying the vibrational fingerprint of nucleobases. Multivariate analysis through principal component analysis (PCA) and regression (PCR) further enhances spectral discrimination and detection sensitivity. The sensor exhibits a limit of detection of 110 pM, falling within the clinically relevant range of salivary SARS-CoV-2 RNA concentrations. Detection performance was assessed in both buffer and artificial saliva, demonstrating the potential of the platform for use with real biological samples. Moreover, the device demonstrates high selectivity, effectively distinguishing between fully matched, mismatched, and random sequences.</div><div>This work highlights the potential of PNA-SERS biosensors for rapid, amplification-free viral RNA detection and offers a promising approach for point-of-care diagnostics in infectious diseases.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100369"},"PeriodicalIF":7.6,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of reusable screen-printed ion-selective electrodes with calibration-free operation 免校准可重复使用的丝网印刷离子选择电极的研制

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-07-24 DOI: 10.1016/j.snr.2025.100367

Pavel Sengupta, Chloe Ramsperger, Dipankar Koley

{"title":"Development of reusable screen-printed ion-selective electrodes with calibration-free operation","authors":"Pavel Sengupta, Chloe Ramsperger, Dipankar Koley","doi":"10.1016/j.snr.2025.100367","DOIUrl":"10.1016/j.snr.2025.100367","url":null,"abstract":"<div><div>The reusable solid-state calibration-free screen-printed ion-selective electrodes (SP-ISEs) have been developed with a carbon paste and PEDOT: PEDOT-S (<em>S</em>=sulfonated) back contact. The calibration-free Na<sup>+</sup> and Ca<sup>2+</sup> SP-ISEs showed a near Nernstian response of 52.1 ± 2.0 mV/log [a<sub>Na+</sub>] and 27.3 ± 0.8 mV/log [a<sub>Ca2+</sub>] respectively while holding a stable intercept for multiple calibrations across batches for 12 h and over 7 days. The unique carbon paste and PEDOT: PEDOT-S copolymer combined back contact on carbon substrate allowed us to fix the intercept with a constant low current treatment across different ionophores for both monovalent Na<sup>+</sup> and divalent Ca<sup>2+</sup> cations. This treatment imparts exceptional potential stability, leading to highly overlapping calibration curves for individual ISEs across multiple measurement cycles, demonstrating their reusability in a calibration-free mode. These SP-ISEs maintained their respective selectivity against major interfering ions and were able to operate reproducibly despite prolonged dry storage without any curing solution, as tested for a total of 28 days. The practical utility of these sensors was validated by analyzing environmental samples, determining Na<sup>+</sup> and Ca<sup>2+</sup> concentrations in tap water (934 ± 37 μM and 194 ± 11 μM respectively) and hydroponics solutions (757 ± 43 μM and 7047 ± 565 μM respectively). This integration of high-performance characteristics on an economical carbon-based substrate creates sensors that are simultaneously affordable enough to be disposable yet stable enough to be reusable, effectively bridging the critical gap between sophisticated laboratory systems and scalable, field-deployable solid ISEs for environmental monitoring applications.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100367"},"PeriodicalIF":7.6,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria 光学生物传感器作为临床检测ESKAPE细菌替代诊断方法的研究进展

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-07-23 DOI: 10.1016/j.snr.2025.100365

Maargavi Singh , Sreelakshmi C․S․ , Chiranjay Mukhopadhyay , Sajan D. George , Pooja Nag , Kapil Sadani

{"title":"Advances in optical biosensors as alternative diagnostics for clinical determination of ESKAPE bacteria","authors":"Maargavi Singh , Sreelakshmi C․S․ , Chiranjay Mukhopadhyay , Sajan D. George , Pooja Nag , Kapil Sadani","doi":"10.1016/j.snr.2025.100365","DOIUrl":"10.1016/j.snr.2025.100365","url":null,"abstract":"<div><div>The global rise of antimicrobial resistance (AMR), manifesting as multidrug-resistant, extremely drug-resistant, and pandrug-resistant pathogens, is causing morbidities which are alarmingly translating to mortalities. The issue is pertinent to low and middle-income countries, which rely heavily on their primary and secondary healthcare setups with severely constrained infrastructure and diagnostics. Traditional and molecular diagnostic methods are effective, but have long turnaround times, are expensive, and require specialized facilities. Due to these constraints, these facilities are usually not present at the primary healthcare centers. This review explores the urgent need for alternative diagnostic strategies beyond conventional pathogen identification and antibiotic susceptibility testing, emphasizing the detection of bacterial metabolites and virulence factors as innovative biomarkers for AMR. This article provides critical insight into tailoring optical biosensor technologies as alternate diagnostics for ESKAPE pathogens in resource-limited settings. It highlights the integration of these biosensing platforms with emerging metabolomics and biomarker profiling technologies, offering a promising route toward point-of-care diagnostics. In addition, incorporating artificial intelligence and machine learning algorithms in signal processing and feature extraction enhances biosensor performance and accelerates diagnostic accuracy. The review critiques the current state of the art in AMR diagnostics and provides strategic inroads for developing robust and deployable diagnostics to help better bacterial infection control.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100365"},"PeriodicalIF":7.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144739515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection 基于分子信标辅助滚动圈扩增的通用荧光侧流条带方法用于即时核酸检测

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-07-23 DOI: 10.1016/j.snr.2025.100363

Xiaopeng Chen , Wentao Wang , Pin Zhang , Qionglin Wang , Kangbo Liu , Fengfang Ding , Xianwei Zhang , Ligong Hou , Yingyu Zhang , Wancun Zhang

{"title":"A universal fluorescent lateral flow strip approach based on molecular beacon-assisted rolling circle amplification for point-of-care nucleic acid detection","authors":"Xiaopeng Chen , Wentao Wang , Pin Zhang , Qionglin Wang , Kangbo Liu , Fengfang Ding , Xianwei Zhang , Ligong Hou , Yingyu Zhang , Wancun Zhang","doi":"10.1016/j.snr.2025.100363","DOIUrl":"10.1016/j.snr.2025.100363","url":null,"abstract":"<div><div>Simple, cost-effective, sensitive, and specific approaches that are capable of enabling point-of-care testing (POCT) are crucial for clinical diagnosis, especially in settings with extremely limited resources. Therefore, a universal, simple, low cost, sensitive, and specific POCT approach called RCA-F-LFS was developed by combining molecular beacon (MB)-assisted rolling circle amplification (RCA) for target amplification with fluorescent lateral flow strips (F-LFS) to enable highly sensitive and visual detection of RCA products. Using <em>Mycoplasma pneumoniae</em> as the detection target, RCA-F-LFS demonstrated high sensitivity (0.1 pM) and specificity, accurately distinguishing it from other pathogenic microorganisms. In addition, RCA-F-LFS enables visual detection of both <em>Mycoplasma pneumoniae</em> DNA and RNA. The detection results of clinical throat swab samples showed that RCA-F-LFS could accurately detect <em>Mycoplasma pneumoniae</em>, with 100 % agreement with current clinical approaches. As a proof of concept, RCA-F-LFS can detect other nucleic acid targets by simply changing the target sequence, offering a robust and versatile strategy for the development of novel clinical POCT detection methods.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100363"},"PeriodicalIF":6.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer-based electrochemical biosensors: Signal transduction mechanisms, application progress, and future trends 基于适配体的电化学生物传感器：信号转导机制、应用进展及未来趋势

IF 7.6

Sensors and Actuators Reports Pub Date : 2025-07-23 DOI: 10.1016/j.snr.2025.100366

Wenji Bao , Gerile Aodeng , Lu Ga , Jun Ai

{"title":"Aptamer-based electrochemical biosensors: Signal transduction mechanisms, application progress, and future trends","authors":"Wenji Bao , Gerile Aodeng , Lu Ga , Jun Ai","doi":"10.1016/j.snr.2025.100366","DOIUrl":"10.1016/j.snr.2025.100366","url":null,"abstract":"<div><div>Aptamer-based electrochemical biosensors synergistically integrate the high molecular recognition specificity of nucleic acid aptamers with the rapid and sensitive signal transduction capabilities of electrochemical interfaces, thereby offering a highly promising platform for advanced bioanalytical applications. Recent advances in nanomaterials, micro/nanofabrication technologies, and signal amplification strategies have markedly improved the analytical performance of these biosensors, enabling ultra-sensitive and highly selective detection of a diverse array of analytes, including disease biomarkers, viral particles, and bacterial pathogens. These biosensors are characterized by low cost, facile miniaturization, and compatibility with point-of-care and on-site diagnostic formats, rendering them attractive for real-world applications. Nevertheless, several critical challenges persist, including maintaining sensor stability under complex biological or environmental sample conditions, achieving reliable multiplexed detection, and establishing standardized fabrication protocols for clinical and environmental deployment. This review provides a comprehensive overview of recent progress in electrochemical transduction mechanisms and their applications, as depicted in Figure 1, and highlights state-of-the-art innovations at the intersection of chemistry, materials science, biomedical engineering, and environmental monitoring. Furthermore, emerging directions—such as artificial intelligence-assisted data interpretation, wearable biosensing systems, and Internet of Things (IoT)-integrated platforms—are discussed to outline future perspectives toward next-generation intelligent and adaptive biosensing technologies.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100366"},"PeriodicalIF":7.6,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0