Sensors and Actuators Reports最新文献

{"title":"Enhancing the sensitivity and accuracy of wearable glucose biosensors: A systematic review on the prospects of mutarotase","authors":"Saminu Abdullahi ,&nbsp;Zhu Yang ,&nbsp;Mohamed Ishag Hassan Gama ,&nbsp;Marwa Omer Mohammed Omer ,&nbsp;Qilin Wang ,&nbsp;Abdulhadi Yakubu ,&nbsp;Zedong Nie","doi":"10.1016/j.snr.2024.100231","DOIUrl":"10.1016/j.snr.2024.100231","url":null,"abstract":"<div><p>Wearable glucose biosensors (WGBs) face significant challenges due to pH, temperature, and skin pollutants affecting glucose detection accuracy by disrupting D-glucose anomeric equilibrium. Although mutarotase (MUT) has historically addressed these challenges, recent research attention on MUT is limited. This systematic review evaluates the performance of biosensors utilizing MUT for enhanced glucose detection. Comprehensive searches across PubMed, Scopus, and Web of Science identified 1,603 studies, of which 13 met PRISMA standards and were selected. Data were extracted and synthesized using pre-designed forms, with results presented through charts and tables. The reviewed studies did not provide clear data about the influence of MUT on the limit of detection (LOD). However, glucose biosensors incorporating MUT demonstrated sensitivity across a broad linear detection range, potentially eliminating the need for sample dilution in some instances. MUT also ensures a more accurate representation of total glucose levels in a sample, achieving complete glucose recovery (100 %) in 6 s in amperometric experiments and within 3-4 min in colorimetric, luminometric, polarimetric, and spectrophotometric studies. Despite stability concerns in 8 % of the studies, MUT proved effective across various pH (5.0–8.5) and temperature (20—37 <span><math><msup><mrow></mrow><mo>∘</mo></msup></math></span>C) ranges. These results highlight the potential of MUT in advancing glucose wearable biosensing technology.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100231"},"PeriodicalIF":6.5,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266605392400047X/pdfft?md5=460e3bc39b854a0aad0ff6b28b7814ad&pid=1-s2.0-S266605392400047X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141949023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An ultrasensitive fluorescence sensing platform for HCV detection based on the T7 isothermal amplification combined with aggregation-induced emission luminogens strategy","authors":"Wuxiu Guo ,&nbsp;Xin Zhu ,&nbsp;Jinchao Li ,&nbsp;Linhai Li","doi":"10.1016/j.snr.2024.100229","DOIUrl":"10.1016/j.snr.2024.100229","url":null,"abstract":"<div><p>Hepatitis C virus infection constitutes a significant global public health concern. Fluorescence technique (FL) is a promising biosensor, however, many conventional dyes exhibit reduced fluorescence or no emission at high concentrations. To break through this shackle, an ultrasensitive AIE-based (aggregation-induced emission) FL sensing platform was developed for HCV detection by using the <u>F</u>LRNAs-based <u>T</u>7 isothermal transcription <u>a</u>mplification <u>R</u>NA diagnosis combined with AIE luminogens (FTAR) strategy. In the FTAR strategy, the T7 transcription amplification process was responsible for the recognition of the target HCV and produced Pepper aptamer, which could form aggregates of nanoparticles with the HBC, an AIEgen, to produce high-intensity FL. The multi-integrated signal amplification strategy led to ultrasensitivity. Moreover, under the optimal experimental conditions, HCV could be assayed in the range of 100aM-100pM. The proposed strategy has been successfully applied in detecting HCV in clinical samples. In summary, this research was the first to utilize aptamer restriction of AIEgens internal rotation to achieve aggregation-induced emission and presented the successful development of a specific, sensitive and rapid FTAR assay for HCV diagnosis. The proposed FTAR platform with facile operation, short analysis time, low-cost as well as excellent quantification ability could provide a promising tool for point-of-care testing (POCT).</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100229"},"PeriodicalIF":6.5,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000456/pdfft?md5=db11efcce0e3fb68a9ed44593ab450db&pid=1-s2.0-S2666053924000456-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ex vivo liquid core fiber photometry with high-resolution 3D printing","authors":"Yu Chang ,&nbsp;Can Wang ,&nbsp;Ke Du","doi":"10.1016/j.snr.2024.100227","DOIUrl":"10.1016/j.snr.2024.100227","url":null,"abstract":"<div><p>High resolution 3D printing emerges as an alternative to microfabrication due to its fine resolution along with one-step manufacturing. Thus, it is broadly used in many fields, such as biological and chemical applications. We introduce such a technique to the design of the optofluidic probe by integrating optics and microfluidics as an ex vivo liquid core fiber photometry. We build the optofluidic probes with various T-shapes and conduct the transmission measurements and the ray tracing simulations, where the results are comparable. Through the transmission and fluorescence measurements, we obtain optimized curl T-shape dimensions of 524 µm wide, ∼50 µm thick, and 350 µm long with longitudinal spaces between them of 260 um. Furthermore, a heightened level of complexity in structure, characterized by a feature size of 25 µm, is attained through the improvement process. We conclude the feasibility of this optofluidic system with two applications: the in vivo-like setting consisting of thyroid biopsy training phantom and human plasma and the ex vivo-like setting consisting of the mice brain slices stained with wheat germ agglutinin (WGA). This prototype is an important step of establishing a 3D printing optofluidic applications for various in vivo research.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100227"},"PeriodicalIF":6.5,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000432/pdfft?md5=7ddbc188d7737ca52f35c36891b6c873&pid=1-s2.0-S2666053924000432-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141852954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanopore sensing and beyond: Electrochemical systems for optically-coupled single-entity studies, stimulus-responsive gating applications, and point-of-care sensors","authors":"Julius Reitemeier ,&nbsp;Jarek Metro ,&nbsp;Kaiyu X. Fu","doi":"10.1016/j.snr.2024.100225","DOIUrl":"10.1016/j.snr.2024.100225","url":null,"abstract":"<div><p>Nanopores play essential roles in biological processes, such as ion channels and pumps in cellular membranes, and in technological applications such as DNA sequencing. Advancements in nanofabrication techniques have enabled the routine integration of nanopores into solid-state devices, resulting in a plethora of analytical applications. This review explores recent developments in nanopore-enabled electrochemical systems, which have transcended traditional resistive pulse sensing to offer novel capabilities in single-entity studies, stimulus-responsive gating, and point-of-care diagnostics. We highlight recent studies on the design and utility of nanopore electrode arrays, which serve as nanocontainers capable of isolating and analyzing single entities, and extend the discussion to hierarchically organized, stimulus-responsive systems that regulate species transport across nanopores, enriching analytes for ultrasensitive detection. In addition, we review the utilization of probe-assisted nanopore sensing, demonstrating its efficacy in selectively binding and detecting target molecules and ions. Finally, we outline future directions for nanopore-based systems to enhance robustness, achieve high-throughput analysis, and incorporate artificial intelligence for materials design and data analysis, promising transformative impacts on diagnostics and biological research.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100225"},"PeriodicalIF":6.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000419/pdfft?md5=4992c1248dbbf3e58110701b170b193f&pid=1-s2.0-S2666053924000419-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141839932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the effect of paper matrix on the electrochemical response of diffusive redox probes","authors":"Dmitry Galyamin ,&nbsp;Eduardo Laborda ,&nbsp;Juan Pablo Esquivel ,&nbsp;Joaquín González ,&nbsp;Neus Sabaté","doi":"10.1016/j.snr.2024.100224","DOIUrl":"10.1016/j.snr.2024.100224","url":null,"abstract":"<div><p>This study evaluates the influence of paper substrate selection on the electrochemical response of an electrochemical paper-based analytical device (ePAD). Various paper substrates commonly employed in this type of devices such as cellulose, nitrocellulose and glass-fibre based medical grade materials are evaluated using chronoamperometric measurements. Both theoretical modelling and experimental analyses are conducted to understand the diffusion behaviour of widely employed two redox probes, [Fe(CN)₆]³⁻ and FcMeOH. Findings show that glass fibre substrates show similar performance to liquid drop conditions while nitrocellulose cause a decrease in current after a short measurement period, mainly due to a thin-layer effect. Cellulose-based substrates decrease the diffusivity of redox species, especially for charged species, indicating potential limitations in their use for chronoamperometric measurements. The study offers valuable insights into the electrochemical behaviour of paper substrates in ePADs, laying the groundwork for future research in this area.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100224"},"PeriodicalIF":6.5,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000407/pdfft?md5=bb215137d80b372495974d62dfaef5da&pid=1-s2.0-S2666053924000407-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141697147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and optimization of a Cr(VI)-Selective Electrode based on a polymeric ß-cyclodextrin membrane modified with sulfur donor groups","authors":"Manuel Ochoa-Pérez ,&nbsp;Jorge Juárez-Gómez ,&nbsp;Sarahy Meneses-Aparicio ,&nbsp;Dafne Sarahia Guzmán-Hernández ,&nbsp;Víctor Lara ,&nbsp;Alberto Rojas-Hernández ,&nbsp;María Teresa Ramírez-Silva","doi":"10.1016/j.snr.2024.100226","DOIUrl":"10.1016/j.snr.2024.100226","url":null,"abstract":"<div><p>This work presents the design and optimization of a new Cr(VI)-selective electrode. The new sensor is based on modifying a glassy carbon electrode with carbon quantum dots, ß-cyclodextrin, and carbon disulfide. The carbon quantum dots aided the electrical properties of the glassy carbon by improving charge transfer; the electropolymerization of ß-cyclodextrin resulted in a polymeric membrane that functions as a recognition element when modified by adding sulfur donor groups to its structure. Modifying the membrane with sulfur atoms gave the sensor excellent selectivity toward Cr(VI) ions. The electrode synthesis was optimized using a 2³ factorial design; the factors studied were pH, the number of cycles in the electropolymerization, and the presence or absence of carbon nanoparticles. Eight different electrodes were constructed, and their potentiometric response to different concentrations of Cr(VI) was evaluated for all of them. The analysis of variance of the experimental design found no significant effect of any factor on the response. However, it suggests a strong interaction between the three factors studied. The sensor that presented the best analytical parameters was synthesized at pH 5 and 50 consecutive cycles in the presence of carbon quantum dots. This new electrode, with its response times of 40 s at different concentrations of the metal ion, exhibiting a slope of (66.0 ± 2.1) mV decade⁻¹ and a detection limit of (5.2 ± 0.1)x10⁻⁷ mol L⁻¹, can be used at pH between 0 and 3 without the effect of hydronium ions. The proposed electrode has good reproducibility and excellent selectivity against various metal ions and has significant advantages over other analysis methods. Its cost, ease of construction, easy handling, ease of operation, ease of storage and transportation, as well as good performance, short response time, and high selectivity make this electrode a valuable tool for easy, fast, and reliable monitoring of Cr(VI) in water samples, contributing to the safety and health of our environment.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100226"},"PeriodicalIF":6.5,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000420/pdfft?md5=5326a7086758dee18ee01ace674f2eca&pid=1-s2.0-S2666053924000420-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced uric acid detection using functionalized multi-walled carbon nanotube/AgNi nanocomposites: A comparative study on screen-printed carbon electrode (SPCE) and fabric-based biosensors","authors":"Yuan Alfinsyah Sihombing ,&nbsp;Uperianti ,&nbsp;Rizky Indah Sari ,&nbsp;Beni Rio Hermanto ,&nbsp;Murni Handayani ,&nbsp;Samuel Priyantoro Kusumocahyo ,&nbsp;Mokhamad Fakhrul Ulum ,&nbsp;Rikson Siburian ,&nbsp;Cepi Kurniawan ,&nbsp;Nuni Widiarti ,&nbsp;Yeni Wahyuni Hartati ,&nbsp;Isa Anshori","doi":"10.1016/j.snr.2024.100223","DOIUrl":"10.1016/j.snr.2024.100223","url":null,"abstract":"<div><p>In the development of biosensors, it is essential to have sensors that provide rapid responses, exhibit high sensitivity and selectivity, and are non-invasive, such as screen-printed carbon electrode-based biosensors. In this study, SPCE-based and fabric-based biosensors were fabricated by modifying the working electrode (WE) surface using functionalized Multi-walled Carbon Nanotube/AgNi nanocomposites (f-MWCNT/AgNi) to enhance the biosensor's performance in detecting uric acid (UA). The successful synthesis of the f-MWCNT/AgNi nanomaterial was confirmed through UV–Vis, Raman, SEM–EDX, and XRD analyses. The f-MWCNT/AgNi nanomaterials were deposited on the WE surface using drop-casting. Subsequently, electrochemical characteristic tests and UA detection performance were conducted using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods. The DPV curves revealed sensitivities of 27.699 μA/mM and 4.638 μA/mM for SPCE-based and fabric-based electrodes, respectively. The limit of detection (LOD) values for UA detection were 0.024 and 0.017 mM, with linearity (R²) 0.997 and 0.999 observed within the linear ranges of 0.05–1.00 and 1.0–5.0 mM, respectively. Both biosensors exhibited strong selectivity for UA against other components, including ascorbic acid, glucose, lactic acid, and ethanol. Based on these parameter values, f-MWCNT/AgNi-modified SPCE and fabric-based electrodes can be promoted as biosensors for uric acid detection.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100223"},"PeriodicalIF":6.5,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000390/pdfft?md5=4444c744795c204087bdf0feb4ba5baa&pid=1-s2.0-S2666053924000390-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141695414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging detection of carbon-based gases with multiple bonds by activating MoO bonding in Na, Sb-codoped NiMoO4","authors":"Si Hoon Jeong ,&nbsp;Gi Hyeon Han ,&nbsp;Gi Hyun Park ,&nbsp;Changhyun Jin ,&nbsp;Jeong Yun Hwang ,&nbsp;Myung Sik Choi ,&nbsp;Se Hwang Kang ,&nbsp;Joon Hyun Baik ,&nbsp;Kyu Hyoung Lee ,&nbsp;Seung Yong Lee","doi":"10.1016/j.snr.2024.100219","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100219","url":null,"abstract":"<div><p>Exhausting carbon-based gases from building construction or comfortable household supplies have caused the main pollution gas species for the indoor air environment. Given the variety of indoor activities, many reports have been challenged to find room-temperature operational and harmless materials. Here, we report that the activating Mo<img>O vibration mode in Na,Sb doped-NiMoO₄ micro-sized powder reveals the high response of CO and VOC, occupying multiple bonds at room temperature. We performed Na and Sb doping in the α-NiMoO₄ crystal structure, such as introducing the Sb in Ni and interstitial doping of Na in around 4 Å of free space in the NiMoO₄ crystal structure that manipulates the partial β-NiMoO₄ phase. The Na,Sb-codoped NiMoO₄ generates the higher Mo<img>O vibration mode from FT-IR measurement and enables carbon-based gas detection with 38 responses to CO and nearly 20 responses to VOC under 20 ppm of each analyte gases environment. Furthermore, the n-type gas sensing behavior of Na,Sb doped-NiMoO₄ chemiresistor exhibits an immediate sensing response instead of a none-of response than intrinsic or single element doped NiMoO₄. These results suggest that Na,Sb codoped-NiMoO₄ is the applicable material for emergent warning against carbon-based gas exposure in the indoor environment. In addition, activating the electrochemical reaction site of MoO₄^–2 in NiMoO₄ is tailorable by a simple doping process.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100219"},"PeriodicalIF":6.5,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000353/pdfft?md5=041d516ae14063cfc21f25858494ab07&pid=1-s2.0-S2666053924000353-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Focused ion beam fabrication of high-resolution electrochemical-electroluminescence coupling bipolar nanoelectrode array sensors","authors":"Guopeng Li ,&nbsp;Rui Hao","doi":"10.1016/j.snr.2024.100220","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100220","url":null,"abstract":"<div><p>High temporal and spatial resolution electrochemical sensor arrays can greatly benefit various parallel sensing applications. Herein, we provide a simple method for the controlled and scaled fabrication of bipolar nanoelectrode arrays (BPnEAs) for high-resolution electrochemical sensing applications. BPnEAs are prepared on silicon nitride film windows through the dual-beam FIB nanofabrication technique. Coupling a conventional electrochemical redox reaction with a tris (2,20-bipyridyl) ruthenium /2-(dibutylamino)ethanol electrochemiluminescence (ECL) system, which is based on a high-viscosity solvent to reduce molecular diffusion, allows for the reporting of conventional electrochemical redox processes with high spatial and temporal resolution. The use of the BPnEA-ECL sensor is demonstrated on 10 × 10 Pt or C BPnEAs for monitoring the reduction of 0.5 M H₂SO₄. BPnEA-ECL sensors containing different electrode materials (Pt and C) are reported for the first time and are used to monitor the reduction of 0.5 M H₂SO₄, thus revealing the difference in electrocatalytic capacities between Pt and C. Subsequently, we reveal the outstanding capabilities of the BPnEA-ECL system for catalyst screening.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100220"},"PeriodicalIF":6.5,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000365/pdfft?md5=9822c1b2795876cb727ed46899430a88&pid=1-s2.0-S2666053924000365-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141594940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultra-fast detection of pathogens and protein biomarkers using a low-cost silicon plasmonic biosensing platform","authors":"Athanasios Manolis ,&nbsp;Christia Eleftheriou ,&nbsp;Mahmoud A. Elrabiaey ,&nbsp;George Tsekenis ,&nbsp;Sabato D'Auria ,&nbsp;Antonio Varriale ,&nbsp;Alessandro Capo ,&nbsp;Maria Staiano ,&nbsp;Bartos Chmielak ,&nbsp;Anna Lena Schall-Giesecke ,&nbsp;Stephan Suckow ,&nbsp;Dimitris Tsiokos","doi":"10.1016/j.snr.2024.100221","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100221","url":null,"abstract":"<div><p>In this paper, we report, for the first time to our knowledge, a new versatile and future proof diagnostic platform for diverse applications and heterogeneous biological targets. This platform relies on a plasmonic-augmented silicon photonic biochip to detect bacteria and protein biomarkers within minutes. To demonstrate the potential of this platform in diverse applications, we demonstrate the detection of two heterogeneous targets, the bacterium <em>Escherichia coli</em> (<em>E. coli</em>) and the molecule C-reactive protein (CRP) using a universal detection method. <em>E. coli</em> is one of the most commonly encountered bacterial pathogens involved in food monitoring, food born infections and water contamination applications, while CRP is a well-established disease severity indicator frequently used in common clinical practice. The biochip used is fully compatible with CMOS semiconductor manufacturing, while it hosts biosensors arrays for any combination of detection assays. Each biosensor exploits a 70 μm long aluminum plasmonic transducer within a silicon nitride waveguide-based Mach–Zehnder Interferometer. Each aluminum surface was silanized and biomodified with specific antibodies. Biosensing experiments revealed that CRP can be detected in diverse sample mediums, and <em>E. coli</em> detection was achieved in buffer at concentrations as low as 10 cells/ml within 13–25 min. The results are in good agreement with preceding numerical simulations. The modular nature of the reported biosensing platform makes it scalable and customizable, allowing nearly any combination of diagnostic tests targeting pathogens and proteins to be integrated on the same biochip for food quality monitoring, environmental monitoring, drug discovery and modern cell therapy manufacturing, or biomedical applications.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100221"},"PeriodicalIF":6.5,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000377/pdfft?md5=653855a9e597a56d3aacc36cd265887b&pid=1-s2.0-S2666053924000377-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}