Sensors and Actuators Reports最新文献_第4页

Advancements in the integration of isothermal nucleic acid amplification methods for point-of-care testing in resource-limited settings

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-01-17 DOI: 10.1016/j.snr.2025.100285

Noemi Bellassai , Roberta D'Agata , Giuseppe Spoto

{"title":"Advancements in the integration of isothermal nucleic acid amplification methods for point-of-care testing in resource-limited settings","authors":"Noemi Bellassai , Roberta D'Agata , Giuseppe Spoto","doi":"10.1016/j.snr.2025.100285","DOIUrl":"10.1016/j.snr.2025.100285","url":null,"abstract":"<div><div>Environmental conditions in low-resource areas exert significant pressure on populations, healthcare personnel, and the tools used for disease diagnosis. Fortunately, advancements in molecular assays now allow for rapid and efficient analysis, leading to effective clinical diagnostics and food safety interventions. Additionally, developing microfluidics and nanotechnologies has facilitated the miniaturization of integrated devices for point-of-care (POC) testing. These innovations have improved sensitivity while significantly reducing the time and cost associated with individual analyzes. This review focuses on the applications of molecular diagnostics for POC devices in low-resource settings, with a particular emphasis on isothermal nucleic acid amplification assays integrated into microfluidic platforms. It begins with an overview of the unmet needs for POC testing and its importance in resource-limited areas. Following this, isothermal amplification methods are described, highlighting their advantages and limitations when implemented in POC platforms, along with real-world applications in biological and food matrices in low- and middle-income countries. We then examine the unique features of POC systems, such as the use of low-cost materials for device fabrication, portability, and detection methods that provide rapid and reliable results near the site of patient care. This capability enables early-stage diagnosis and improved clinical management in low-resource environments.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100285"},"PeriodicalIF":6.5,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flexible touch and gesture recognition system for curved surfaces with machine learning for assistive applications

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-01-15 DOI: 10.1016/j.snr.2025.100284

Gitansh Verma , Shrutidhara Sarma , Eugen Koch , Andreas Dietzel

{"title":"Flexible touch and gesture recognition system for curved surfaces with machine learning for assistive applications","authors":"Gitansh Verma , Shrutidhara Sarma , Eugen Koch , Andreas Dietzel","doi":"10.1016/j.snr.2025.100284","DOIUrl":"10.1016/j.snr.2025.100284","url":null,"abstract":"<div><div>Touch is a fundamental mode of human-machine interaction and ability to monitor tactile pressure, recognize gestures and location of touch are crucial for touch-based technologies. However, achieving reliable touch sensing on curved surfaces remains challenging as flexing often disrupts the stability of sensor outputs and diminishes sensitivity, especially in dynamic environments. This study presents the development of a flexible multi-element touch sensing patch that can monitor its bending state as well as detect pressure with a sensitivity of 0.827 kPa<sup>−1</sup>. The patch is fabricated using resistive strain sensors, screen printed onto a PET sheet with a foam backing. Evaluation electronics were integrated to ensure stable, noise-free signal acquisition, and output was processed with machine learning (ML) algorithms to classify gestures such as single and double finger taps, swipes, and touch locations, with 93 % accuracy, on both flat and curved surfaces. Based on the identified gesture, the system enables users to type text or control external devices with minimal physical effort. Its scalable fabrication, high sensitivity, mechanical resilience and seamless ML integration establishes it as a powerful and efficient tool for assistive technologies, designed to support individuals with limited speech and mobility, such as those with quadriplegia or paralysis.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100284"},"PeriodicalIF":6.5,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bipolar electrochemically generated fluorescence detector for microchip electrophoresis with and without a potentiostat: Application to reducible analyte detection

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-01-14 DOI: 10.1016/j.snr.2025.100283

Manjula B. Wijesinghe , Indika K. Warnakula , Dulan B. Gunasekara , Susan M. Lunte

{"title":"Bipolar electrochemically generated fluorescence detector for microchip electrophoresis with and without a potentiostat: Application to reducible analyte detection","authors":"Manjula B. Wijesinghe , Indika K. Warnakula , Dulan B. Gunasekara , Susan M. Lunte","doi":"10.1016/j.snr.2025.100283","DOIUrl":"10.1016/j.snr.2025.100283","url":null,"abstract":"<div><div>Microchip electrophoresis (ME) is a separation method that makes it possible to quickly analyze multiple species in small volume samples in a miniaturized format. Two commonly employed detection approaches for ME are electrochemistry and fluorescence. Electrochemical detection can be employed for direct detection of analytes via redox chemistry, while fluorescence generally offers lower limits of detection but often requires analyte derivatization. The present study uses bipolar electrochemistry to couple the two detection methods into a single device as a detector for that encompasses the advantages of both techniques. This system employs a closed bipolar electrode (BPE) that converts the cathodic amperometric current produced at the separation (sensing) pole to a fluorescence response generated at the reporting pole. Two model analytes, benzoquinone and resazurin, were separated and detected using ME with reductive amperometric detection at the sensing pole of the BPE. A potentiostat was used to control the bipolar electrode. The response was then converted to fluorescence through the oxidation of 2,7-dichlorodihydrofluorescein in the reporting channel. This system was subsequently modified to run without a potentiostat by using the separation voltage to bias the BPE sensing pole. A voltage applied across the reporting channel was used to generate electroosmotic flow as well as bias the corresponding pole of the BPE. This potentiostat-free mode was evaluated for the determination of 3-nitro-<span>l</span>-tyrosine, a reducible biomarker of oxidative stress. Lastly, a sacrificial electrochemically active species, Trolox®, was incorporated into the reporting channel to compensate for background current at the sensing channel and lower the limits of detection.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100283"},"PeriodicalIF":6.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous flow acoustofluidics in wall-less capillary bridge channels

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-01-08 DOI: 10.1016/j.snr.2024.100275

Sadaf Maramizonouz , Jeremy J. Hawkes , Mohammad Rahmati , Yong-Qing Fu

{"title":"Continuous flow acoustofluidics in wall-less capillary bridge channels","authors":"Sadaf Maramizonouz , Jeremy J. Hawkes , Mohammad Rahmati , Yong-Qing Fu","doi":"10.1016/j.snr.2024.100275","DOIUrl":"10.1016/j.snr.2024.100275","url":null,"abstract":"<div><div>In this study, the manipulation, patterning, and alignment of yeast particles were successfully demonstrated inside a continuous flow capillary bridge channel. A numerical model is presented to explore various frequencies and channel configurations, and the numerical results are compared to the experimental findings. The numerical model produced results in excellent agreement with the experimental data. Yeast particles were aligned in linear patterns while continuously flowing parallel to the air-water interfaces of the capillary bridge channel. The width of the fluid-guide significantly affected the acoustic pressure fields and thus the quality of particle patterning. Most frequencies can achieve particle alignment inside the capillary bridge channel, while the liquid flow straightens the particle line. Studies of ultrasound transfer from a solid wave-guide into the liquid in a capillary bridge channel have previously shown that some nodes which formed in the waveguide will cross the solid-fluid interface into the water of the capillary bridge. The nodes formed by this extension mechanism are not easily distinguishable from nodes formed by a resonance in the liquid since particles are attracted to them in the same way and the node-to-node separation is often similar. This paper provides further foundation for the new node extension concept and identifies some of its unique features.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100275"},"PeriodicalIF":6.5,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistive pulse sensing of pre-nucleation activities during single-entity lysozyme crystallization on single nanopipettes

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-01-06 DOI: 10.1016/j.snr.2025.100281

Yusuff Balogun , Ruoyu Yang , Gangli Wang

{"title":"Resistive pulse sensing of pre-nucleation activities during single-entity lysozyme crystallization on single nanopipettes","authors":"Yusuff Balogun , Ruoyu Yang , Gangli Wang","doi":"10.1016/j.snr.2025.100281","DOIUrl":"10.1016/j.snr.2025.100281","url":null,"abstract":"<div><div>The formation of cluster aggregates in a (super)saturated solution prior to protein nucleation is crucial to overcoming the thermodynamic energy barrier which enables further growth of single crystals. This process is important for single crystal growth, separation and energy conversion among other important applications. For structural determination of biomacromolecules, neutron crystallography holds unique advantages in resolving hydrogen/proton over other structure determination techniques but faces technical obstacles in requiring large high-quality single crystals and preferentially hydrogen-deuterium exchanges. Herein, we explore protein nucleation in heavy water (D<sub>2</sub>O) via nanopore-based resistive pulse sensing, with lysozyme as prototype. By controlling localized supersaturation and phase transition at a nanopore through adjusting the potential waveform, a single protein crystal can be grown. Our focus is on understanding the translocation and/or transformation of protein aggregates through nanopores prior to the irreversible nucleation. As expected, higher protein concentrations tend to facilitate nucleation and growth of a single protein crystal with higher supersaturation, consistent with bulk experiments. At lower protein concentrations, individual current spikes are resolved as characteristic single-entity events in resistive pulse sensing. Those transient events are potential-dependent characterized by the peak amplitude, duration and area/charges. Statistical analysis reveals both translocation of protein oligomers and their transformation or further aggregation. This study represents the first step toward elucidating valuable insights into the dynamics of protein translocation and aggregation in heavy water and demonstrates the potential of using nanopores in the detection and characterization of dynamic phase transitions at single-event levels.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100281"},"PeriodicalIF":6.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements in Point-of-Care Detection of Contaminants and Biomarkers in Human Breast Milk: A comprehensive review

IF 6.5

Sensors and Actuators Reports Pub Date : 2025-01-01 DOI: 10.1016/j.snr.2024.100280

Shruti Janakiraman , Rinky Sha , Naresh Kumar Mani

{"title":"Recent advancements in Point-of-Care Detection of Contaminants and Biomarkers in Human Breast Milk: A comprehensive review","authors":"Shruti Janakiraman , Rinky Sha , Naresh Kumar Mani","doi":"10.1016/j.snr.2024.100280","DOIUrl":"10.1016/j.snr.2024.100280","url":null,"abstract":"<div><div>Breastmilk is an essential source of nutrients for newborn babies. It contains amino acids, nucleic acids and the essential immunological components required for the neonate to survive. Along with a number of other contaminants, it also includes several biomarkers, which are signs of a diseased state of the host. Thus, it is obvious that by examining the contents of this bodily fluid and how it affects the newborn, we can provide better healthcare solutions to both the mother and the child. There are several methods by which these analytes can be analysed, and they can be broadly classified in two categories; conventional and point-of-care based techniques. These approaches have benefits and drawbacks and are both in use today. However, point-of-care procedures are becoming increasingly popular in the scientific community because of their portability, affordability, and shorter turnaround times. In this review, the significance of employing these techniques to examine the intricacy of breastmilk as well as the contaminants and biomarkers that it contains will be covered. Wherever appropriate, the future of point-of-care approaches, including wearable sensors, has also been discussed, along with the significance, drawbacks, and advantages of combining these two technologies to recognize contaminants and biomarkers in breast milk.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100280"},"PeriodicalIF":6.5,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast and sensitive virus detection using a plasmonic sensor that integrates electrokinetically assisted sampling and surface-enhanced Raman scattering

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-12-26 DOI: 10.1016/j.snr.2024.100273

Shamim Azimi , Maryam Moridsadat , Bhavin Shastri , Bruce W. Banfield , Carlos Escobedo , Aristides Docoslis

{"title":"Fast and sensitive virus detection using a plasmonic sensor that integrates electrokinetically assisted sampling and surface-enhanced Raman scattering","authors":"Shamim Azimi , Maryam Moridsadat , Bhavin Shastri , Bruce W. Banfield , Carlos Escobedo , Aristides Docoslis","doi":"10.1016/j.snr.2024.100273","DOIUrl":"10.1016/j.snr.2024.100273","url":null,"abstract":"<div><div>We present a novel plasmonic biosensing method for on-chip detection on viral particles featuring a microelectrode platform that integrates accelerated sampling of virus with surface-enhanced Raman scattering (SERS). We show experimentally that our approach can produce spectacular results owing to the unique incorporation of two key features: (1) Concentration amplification of virus on the SERS-active substrate; (2) Local plasmonic activity enhancement due to the targeted superimposition of silver nanoparticles on the captured virus sites. When tested for the detection of the M13 bacteriophage our “sandwich” assay yielded excellent reproducibility (signal variation <6 %) and a very low limit of detection (1.13 × 10<sup>2</sup> pfu/ml). Compared with the performance of our standard SERS substrates, SERS signals stronger by at least one order of magnitude are typically achieved. In addition to experimental results, our work also includes finite element (COMSOL Multiphysics) and finite-difference time-domain (FDTD) simulations that provide insights into the mechanisms of concentration amplification and plasmonic activity enhancement, respectively.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100273"},"PeriodicalIF":6.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Platinum nanoparticles decorated on electrodeposited Ti3C2Tx MXene nanosheets as an ultrasensitive biosensor for CA15–3 detection

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-12-26 DOI: 10.1016/j.snr.2024.100278

Sepideh Shafaei , Rahim Mohammad-Rezaei , Balal Khalilzadeh , Ibrahim Isildak

{"title":"Platinum nanoparticles decorated on electrodeposited Ti3C2Tx MXene nanosheets as an ultrasensitive biosensor for CA15–3 detection","authors":"Sepideh Shafaei , Rahim Mohammad-Rezaei , Balal Khalilzadeh , Ibrahim Isildak","doi":"10.1016/j.snr.2024.100278","DOIUrl":"10.1016/j.snr.2024.100278","url":null,"abstract":"<div><div>In this study, an efficient and ultrasensitive biosensor based on platinum nanoparticles decorated on MXene nanosheets (NSs) was reported for the detection of CA15–3 breast cancer biomarker. In order to increase the accuracy and reproducibility of the fabricated biosensor, MXene NSs and platinum nanoparticles (PtNPs) were electrochemically deposited on the surface of glassy carbon electrode (MXene-Pt/GCE). The synergistic effect of MXene and PtNPs caused an increased conductivity, fast electron transfers, amplified sensitivity and improved stabilization of streptavidin and CA15–3 antibody on the electrode surface. According to the electrochemical results, the electroactive surface area of MXene-Pt/GCE was obtained 0.1345 cm<sup>2</sup> which was remarkably more than GCE. The detection limit and linear range for the developed CA15–3 breast cancer biosensor were 1 nU and 1 to 100 nU, respectively. Based on the data, the proposed biosensor exhibited unique reproducibility, stability, and selectivity which can be differentiate between the healthy serum samples and patients suffering from breast cancer.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100278"},"PeriodicalIF":6.5,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cortisol detection using a Long Period Fiber Grating Immunosensor coated with Graphene Oxide

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-12-25 DOI: 10.1016/j.snr.2024.100279

Simone Soares , Ambra Giannetti , Flavio Esposito , Lucia Sansone , Anubhav Srivastava , Stefania Campopiano , Michele Giordano , Margarida Facão , Nuno F. Santos , Agostino Iadicicco , Carlos Marques , Francesco Chiavaioli

{"title":"Cortisol detection using a Long Period Fiber Grating Immunosensor coated with Graphene Oxide","authors":"Simone Soares , Ambra Giannetti , Flavio Esposito , Lucia Sansone , Anubhav Srivastava , Stefania Campopiano , Michele Giordano , Margarida Facão , Nuno F. Santos , Agostino Iadicicco , Carlos Marques , Francesco Chiavaioli","doi":"10.1016/j.snr.2024.100279","DOIUrl":"10.1016/j.snr.2024.100279","url":null,"abstract":"<div><div>Recirculating Aquaculture Systems (RAS) have revolutionized the protein production sector in aquaculture, leading to significant growth and expansion of the industry. Despite the success of RAS in aquaculture, there are challenges related to stress in fish raised in these systems, which can impact their food intake, growth, and overall well-being. One of the major limitations in the aquaculture industry is the lack of smart sensors for real-time detection of stress hormones like cortisol, hindering our ability to understand and effectively manage the welfare of fish in these systems. In this work, a graphene oxide (GO) coated long period grating (LPG) was fabricated into a double-clad optical fiber (DCF) with W-shaped refractive index profile. The working point of the device was tuned to the mode transition region to enhance its sensitivity against outer medium changes. It was further integrated into a microfluidic system and the fiber surface was functionalized with specific anti-cortisol antibodies for the detection of cortisol. Finally, the performance of this immunosensor was evaluated for a cortisol concentration range of 0.01 ng/mL to 100 ng/mL, a wide working range of concentrations of relevant interest, achieving a limit of detection (LOD) of 0.06 ng/mL. Moreover, a selectivity test using testosterone and glucose as interfering substances was carried out.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100279"},"PeriodicalIF":6.5,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143158250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform

IF 6.5

Sensors and Actuators Reports Pub Date : 2024-12-24 DOI: 10.1016/j.snr.2024.100276

Supawee Inlumphan , Winadda Wongwiriyapan , Narathon Khemasiri , Prapakorn Rattanawarinchai , Piyawan Leepheng , Panuwat Luengrojanakul , Tuksadon Wuttikhun , Michiko Obata , Masatsugu Fujishige , Kenji Takeuchi , Mayuree Phonyiem Reilly , Teerayut Uwanno , Mati Horprathum , Supanit Porntheeraphat , Kannika Sitthisuwannakul , Supranee Phanthanawiboon , Annop Klamchuen

{"title":"Laser-induced graphene electrochemical immunosensors for rapid and sensitive serological detection: A case study on dengue detection platform","authors":"Supawee Inlumphan , Winadda Wongwiriyapan , Narathon Khemasiri , Prapakorn Rattanawarinchai , Piyawan Leepheng , Panuwat Luengrojanakul , Tuksadon Wuttikhun , Michiko Obata , Masatsugu Fujishige , Kenji Takeuchi , Mayuree Phonyiem Reilly , Teerayut Uwanno , Mati Horprathum , Supanit Porntheeraphat , Kannika Sitthisuwannakul , Supranee Phanthanawiboon , Annop Klamchuen","doi":"10.1016/j.snr.2024.100276","DOIUrl":"10.1016/j.snr.2024.100276","url":null,"abstract":"<div><div>Here, we present a diagnostic on a chip platform based on laser-induced graphene (LIG) electrochemical immunosensors for serological detection. The electrochemical immunosensors are fabricated through CO<sub>2</sub> laser induction on polyimide (PI) sheets. Optimal electrochemical activity of LIG electrodes is obtained under optimized conditions of laser fluence. To verify the application, the serological detection platform was demonstrated. After functionalization with dengue virus (DENV) antigen, the LIG electrochemical immunosensors are able to sense the presence of mouse anti-flavivirus monoclonal (4G2) antibody in a wide linear working range of 25–20,000 ng/ml with the limit of detection (LOD) of 17.41 ng/ml. A specific recognition with 4G2 antibodies against with media protein and isotype is confirmed. Furthermore, the reliability of LIG electrochemical immunosensors compared to conventional enzyme-linked immunosorbent assay (ELISA) is verified through the NS1 antibodies identification in human blood serum clinical samples at room temperature. Our results highlight that the LIG-based electrode is a promising platform for electrochemical immunosensors, aimed at developing reliable and practical diagnostic tools for serological detection. These tools enable early diagnosis of infectious diseases, as well as non-invasive and rapid screening.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"9 ","pages":"Article 100276"},"PeriodicalIF":6.5,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143157708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0