Sensors and Actuators Reports最新文献

{"title":"Zeptomolar detection of analytes via nanoplasmonic biochips based on optimized L-shaped gold nanogratings","authors":"Francesco Arcadio ,&nbsp;Annabella la Grasta ,&nbsp;Luigi Zeni ,&nbsp;Francesco Dell’Olio ,&nbsp;Nunzio Cennamo","doi":"10.1016/j.snr.2026.100442","DOIUrl":"10.1016/j.snr.2026.100442","url":null,"abstract":"<div><div>Label-free, chip-scale biosensors that exploit periodic nanophotonic and nanoplasmonic structures convert binding-induced refractive-index changes into sharp spectral signatures, enabling compact biochemical and hormone testing. Advances in metasurface and nanograting architectures have improved spectral quality factors and bulk sensitivity; however, for small molecules, pushing label-free limits of detection into the zeptomolar regime in fiber-compatible, low-cost formats remains difficult because of the intrinsically weak mass load and the trade-off between near-field confinement and resonance linewidth. Here, we show an electron-beam-patterned metasurface of L-shaped gold nanogratings on PMMA, engineered to support a Fano-shaped localized surface plasmon resonance, interrogated in transmission through polymer optical fibers and a 3D-printed liquid cell. Finite-element modeling guided the geometry, and the fabricated device exhibits a bulk sensitivity of <span><math><mrow><mn>1001</mn><mo>±</mo><mn>61</mn><mspace></mspace><mi>nm</mi><mspace></mspace><msup><mrow><mi>RIU</mi></mrow><mrow><mo>−</mo><mn>1</mn></mrow></msup></mrow></math></span> over <span><math><mrow><mi>n</mi><mo>=</mo><mn>1</mn><mo>.</mo><mn>332</mn></mrow></math></span>–1.352. After immobilizing estrogen receptor <span><math><mi>α</mi></math></span>, the platform quantifies <span><math><mi>β</mi></math></span>-estradiol from 0.05 to <span><math><mrow><mn>100</mn><mspace></mspace><mi>aM</mi></mrow></math></span>; Langmuir analysis yields a low-dose slope of <span><math><mrow><mn>9</mn><mo>.</mo><mn>18</mn><mo>±</mo><mn>2</mn><mo>.</mo><mn>9</mn><mspace></mspace><mi>nm</mi><mspace></mspace><msup><mrow><mrow><mo>(</mo><mi>aM</mi><mo>)</mo></mrow></mrow><mrow><mo>−</mo><mn>1</mn></mrow></msup></mrow></math></span> and an instrumental limit of detection of <span><math><mrow><mn>63</mn><mo>±</mo><mn>20</mn><mspace></mspace><mi>zM</mi></mrow></math></span> (<span><math><mrow><mn>3</mn><mi>σ</mi><mo>/</mo><msub><mrow><mi>S</mi></mrow><mrow><mi>low</mi></mrow></msub></mrow></math></span>), while dihydrotestosterone and bovine serum albumin controls induce negligible shifts. These results indicate that deterministic metasurface design coupled to fiber-based interrogation can deliver ultrasensitive, chip-scale steroid assays without labels and provide a general route to portable photonic biosensing. These features are directly relevant to ultra trace hormone monitoring in clinical endocrinology and fertility medicine, as well as on site surveillance of endocrine disrupting compounds in natural/industrial waters, where the fiber coupled readout and 3D printed liquid cell facilitate portable, low footprint deployment outside laboratory environments.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"11 ","pages":"Article 100442"},"PeriodicalIF":7.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146034267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Review of flexible touchpad technology: From principles to applications","authors":"Youliang Lv ,&nbsp;Tao Chen ,&nbsp;Jun Li ,&nbsp;Bo Zhu ,&nbsp;Yang Wang ,&nbsp;Chunping Ma ,&nbsp;Fei Wang","doi":"10.1016/j.snr.2025.100419","DOIUrl":"10.1016/j.snr.2025.100419","url":null,"abstract":"<div><div>Flexible touchpads are rapidly emerging as pivotal interfaces for wearable electronics, soft robotics, and embodied intelligence, enabling deformable, conformal, and multimodal human-machine interaction, yet their commercial adoption remains hindered by persistent challenges despite significant academic and technological progress. This review provides a quantitative, critical, and system-oriented analysis to identify key bottlenecks and propose actionable strategies for scalable deployment. It begins with examining dominant working mechanisms and benchmarking representative devices across critical performance metrics, including pressure range, sensitivity, resolution, response time, and mechanical durability. Then it traces a technology roadmap from material selection and routing architectures to fabrication methods, with applications spanning gesture recognition, health monitoring, and in-vehicle interaction, while emphasizing system-level considerations such as integration with flexible electronics and power efficiency. Key challenges are distilled, including material fatigue and interfacial delamination under prolonged deformation, signal drift and crosstalk in large-area arrays, packaging robustness and washability for daily use, and the absence of standardized testing protocols, alongside proposed solutions such as mechanically graded structures, durable interfacial bonding, optimized low-wire-count routing, and algorithm-hardware co-design. The review offers a foundational guide for researchers and engineers aiming to bridge the gap between flexible touchpad innovation and practical deployment.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"11 ","pages":"Article 100419"},"PeriodicalIF":7.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145787826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topology-controlled plasmonic nanocapsules enable more sensitive quantitative SERS detection of zinc","authors":"Ting Zhou ,&nbsp;Bernardo A. Nogueira ,&nbsp;Laura Rodríguez-Lorenzo ,&nbsp;Pilar Rivera-Gil","doi":"10.1016/j.snr.2026.100479","DOIUrl":"10.1016/j.snr.2026.100479","url":null,"abstract":"<div><div>Quantifying labile Zn(II) in complex biological media remains challenging for optical nanosensors because matrix effects and SERS substrate heterogeneity distort signal intensities. We report here topology-controlled plasmonic Au@SiO₂ nanocapsules functionalized with a thiolated terpyridine (TPY) Raman reporter as ratiometric SERS nanosensors for quantitative zinc detection. The denser apparent AuNP loading and red-shifted/broadened UV–Vis-NIR response are consistent with stronger interparticle plasmon coupling and improved overlap with 785 nm excitation. Labile zinc binding is quantified using an integrated-band ratio in the 1000–1050 cm^-1 region, enabling robust calibration in a zinc-depleted cell-culture-derived matrix used as a controlled biologically relevant calibration medium. The optimized formulation shows improved linearity (R² up to 0.99) over 10^–4 – 10^–12 M Zn(II) and a condition-specific statistical LOD of 1.7 × 10^–13 M (10^–12.77 M), outperforming our earlier formulation. Density functional theory calculations support vibrational assignment of Zn-TPY marker bands and guide future selection for quantification. These results establish nanocapsule topology as an effective tuning parameter for improving quantitative Zn(II) readouts under controlled cell-culture-medium conditions.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"11 ","pages":"Article 100479"},"PeriodicalIF":7.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148183642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Centrifugal microfluidic systems for cancer cell separation: Advances, challenges, and applications","authors":"Alireza Farahinia ,&nbsp;Wenjun Zhang ,&nbsp;Ildiko Badea","doi":"10.1016/j.snr.2025.100387","DOIUrl":"10.1016/j.snr.2025.100387","url":null,"abstract":"<div><div>Cancer remains a leading global health challenge, with circulating tumor cells (CTCs) playing a pivotal role in metastasis and disease progression. Efficient detection and isolation of CTCs are essential for early diagnosis, therapeutic monitoring, and the advancement of personalized treatment strategies. However, their extreme rarity in peripheral blood presents significant technical challenges for reliable enrichment and analysis. Centrifugal microfluidic systems, or lab-on-a-disc (LOCD) platforms, offer a promising solution by enabling automated, high-throughput, and cost-effective separation of rare cancer cells with minimal manual intervention. This review provides a comprehensive analysis of recent advances in centrifugal microfluidic technologies, with a focus on cancer cell separation for diagnostics and the challenges of clinical translation. Particular attention is given to the optimization of separation techniques, improvements in microchannel design, and strategies to minimize contamination and cell damage while enhancing purity and yield. We critically compare label-free, affinity-based, and hybrid separation approaches, and examine how material selection, surface functionalization, automation, and integrated detection modules in-fluence device performance. Clinical relevance is emphasized throughout, including examples of real patient applications, regulatory challenges, and translational barriers. Furthermore, we propose future directions to address persistent limitations such as clogging, limited specificity, and standardization. While routine clinical implementation remains complex, recent innovations have significantly improved system robustness, reproducibility, and accessibility. This review serves as a resource for researchers and clinicians, summarizing the current state of the field and outlining the path forward for the next generation of centrifugal microfluidic systems tailored for cancer cell separation.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100387"},"PeriodicalIF":7.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145262462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual nanoparticle-enhanced biophotonic fiber sensor for highly sensitive and selective detection of pseudomonas aeruginosa in food safety applications","authors":"Qi Zhang ,&nbsp;Ragini Singh ,&nbsp;Rui Min ,&nbsp;Ben Niu ,&nbsp;Wilfried Blanc ,&nbsp;Bingyuan Zhang ,&nbsp;Santosh Kumar","doi":"10.1016/j.snr.2025.100409","DOIUrl":"10.1016/j.snr.2025.100409","url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>), as a foodborne pathogen, is often underestimated in the field of food safety. In light of this, the development of a sensor for detecting <em>P. aeruginosa</em> that possesses both high sensitivity and high selectivity is of great significance for food safety monitoring and the medical health field. Based on the localized surface plasmon resonance (LSPR) effect, this study designs and constructs a novel biophotonic fiber sensor using gold nanoparticles (AuNPs) and platinum nanoparticles (PtNPs) with significant LSPR effects, aiming to efficiently detect <em>P. aeruginosa</em>. The core of the sensor is to fix specific antibodies on the surface of nanoparticles and achieve detection by monitoring the LSPR spectral shift triggered by the binding of bacteria to antibodies. The sensor demonstrates a low detection limit of 2.6 CFU/mL, which represents a significant advantage in the current technological context. Furthermore, this study enhances the selectivity of the sensor by functionalizing antibodies to meet the specific requirements for screening <em>P. aeruginosa</em> in food. To comprehensively evaluate the performance of the sensor, we tested its stability, repeatability, and selectivity, with results showing excellent performance. The formation of biofilm by <em>P. aeruginosa</em> is one of its major virulence factors, and our sensor can also accurately detect the presence of biofilm. Further application of the sensor in tests simulating real-world environments indicates that the sensor has the potential for practical application. These advantages not only confirm the high efficiency of the proposed sensor in accurately detecting <em>P. aeruginosa</em> but also highlight its significant application prospects in the field of food safety detection.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100409"},"PeriodicalIF":7.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145614523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine learning in cancer prognostic and diagnostic biomarkers: A promising approach for early cancer detection","authors":"Pegah Vosoughi ,&nbsp;Seyed Morteza Naghib ,&nbsp;Ghasem Takdehghan","doi":"10.1016/j.snr.2025.100385","DOIUrl":"10.1016/j.snr.2025.100385","url":null,"abstract":"<div><div>Cancer is a multifaceted disease that arises from both genetic and epigenetic alterations that disturb the regulation of cell growth and programmed cell death, also known as apoptosis. This imbalance results in unchecked cell proliferation, ultimately leading to tumor formation. Globally, cancer presents a significant public health issue, causing millions of deaths each year and placing immense pressure on healthcare systems around the world. Early cancer detection is crucial, as it dramatically improves the chances of successful treatment. Consequently, advancements in biomarker research and diagnostic technologies are vital for creating more effective detection strategies that reduce the impact of cancer on patients and healthcare systems. For biomarkers to be helpful in clinical practice, they must thoroughly evaluate their analytical and clinical validity and utility. Analytical validity focuses on the technical accuracy of biomarker assays, encompassing aspects such as sample handling, assay methods, and the reliability of the results. In recent years, machine learning (ML) and deep learning (DL) have become crucial tools for identifying biomarkers in healthcare engineering. The advancements in artificial intelligence (AI) have expanded these technologies' applications across various healthcare domains. This research highlights the latest innovations and methodologies, including advanced feature selection techniques and ML/DL algorithms. This review emphasizes the transformative impact of ML technologies in biomarker discovery. It also encompasses multiple facets of ML-based platforms, detailing their applications and effectiveness in biomarker discovery. Using sophisticated algorithms, these advanced computational methods allow researchers to analyze various data types, from genomic sequences to proteomic profiles. This approach facilitates the identification of novel biomarkers, enhances our understanding of cancer biology, and paves the way for improved patient care through personalized medicine. This review highlights exciting opportunities for future research, encouraging continuous innovation and collaboration across disciplines among data scientists, healthcare experts, and academic researchers.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100385"},"PeriodicalIF":7.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145154508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comprehensive review of nanozyme construction strategies and their applications in environmental analysis","authors":"Samar H. Elagamy ,&nbsp;Reem H. Obaydo ,&nbsp;Abdulsalam Ashkar ,&nbsp;Hayam M. Lotfy","doi":"10.1016/j.snr.2025.100394","DOIUrl":"10.1016/j.snr.2025.100394","url":null,"abstract":"<div><div>Nanozymes NZs have gained increasing attention in environmental analysis due to their robust performance, cost-effectiveness, and resistance to harsh conditions compared to natural enzymes. Recent developments in nanozyme construction focus not only on enhancing catalytic activity and selectivity but also on adopting greener synthesis approaches such as using plant-based precursors, and low-energy methods in line with the principles of green analytical chemistry GAC. This review provides a comprehensive overview of the types of NZs, including metal-based, carbon-based, and hybrid system with nanomaterials, metal–organic frameworks (MOFs) based systems, as well as molecularly imprinted polymers MIPs. Additionally, it discusses strategies for hybridizing MIPs with MOFs as a powerful approach to engineer advanced NZs with enhanced selectivity, stability, and catalytic activity. The review also covers key aspects of nanozyme development, including the catalytic mechanisms, detection modes, synthetic methods, and green synthesis approaches. The environmental applications of these systems, including pollutant detection and heavy metal ion sensing, are discussed in detail. Finally, current limitations, challenges, and perspectives for the future development of green NZs in real-world environmental monitoring are addressed.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100394"},"PeriodicalIF":7.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Broad spectrum UV–Vis-NIR photodetector based on mechanically exfoliated MoSSe thin film","authors":"Feng Zhou ,&nbsp;Siyuan Yu ,&nbsp;Ming Wu ,&nbsp;Xuanqi Zhong ,&nbsp;Xiaoxian Song ,&nbsp;Haiting Zhang","doi":"10.1016/j.snr.2025.100350","DOIUrl":"10.1016/j.snr.2025.100350","url":null,"abstract":"<div><div>In recent years, binary transition metal dichalcogenides (TMDs) have emerged as a highly promising class of optoelectronic materials, due to their exceptional stability and optoelectronic properties. However, binary TMDs are typically associated with deep defect states, impeding the enhancement of the device's photo responsivity and response time. The alloying of binary two-dimensional (2D) materials provides an effective solution to this problem. A broadband metal-semiconductor-metal (MSM) photodetector based on a mechanically exfoliated MoSSe thin film is reported. The device shows excellent responsivity and fast response at UV–Vis-NIR wavelengths (375–1550 nm). Under the irradiation of the 532 nm laser, the device shows a photo responsivity (R) of 15 mA/W, a specific detectivity (D₁*) of 6.91 × 10⁹ Jones and D₂* of 6.48 × 10⁹ Jones, a response rise time of 43 ms, and a fall time of 38 ms. Moreover, the photoelectric imaging of a photodetector at 1550 nm was achieved by a single-site scanning imaging system. This research has contributed to the field of 2D materials, particularly in MoSSe for broadband photodetectors and photoelectric imaging.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100350"},"PeriodicalIF":6.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of reusable screen-printed ion-selective electrodes with calibration-free operation","authors":"Pavel Sengupta,&nbsp;Chloe Ramsperger,&nbsp;Dipankar Koley","doi":"10.1016/j.snr.2025.100367","DOIUrl":"10.1016/j.snr.2025.100367","url":null,"abstract":"<div><div>The reusable solid-state calibration-free screen-printed ion-selective electrodes (SP-ISEs) have been developed with a carbon paste and PEDOT: PEDOT-S (<em>S</em>=sulfonated) back contact. The calibration-free Na⁺ and Ca²⁺ SP-ISEs showed a near Nernstian response of 52.1 ± 2.0 mV/log [a_Na+] and 27.3 ± 0.8 mV/log [a_Ca2+] respectively while holding a stable intercept for multiple calibrations across batches for 12 h and over 7 days. The unique carbon paste and PEDOT: PEDOT-S copolymer combined back contact on carbon substrate allowed us to fix the intercept with a constant low current treatment across different ionophores for both monovalent Na⁺ and divalent Ca²⁺ cations. This treatment imparts exceptional potential stability, leading to highly overlapping calibration curves for individual ISEs across multiple measurement cycles, demonstrating their reusability in a calibration-free mode. These SP-ISEs maintained their respective selectivity against major interfering ions and were able to operate reproducibly despite prolonged dry storage without any curing solution, as tested for a total of 28 days. The practical utility of these sensors was validated by analyzing environmental samples, determining Na⁺ and Ca²⁺ concentrations in tap water (934 ± 37 μM and 194 ± 11 μM respectively) and hydroponics solutions (757 ± 43 μM and 7047 ± 565 μM respectively). This integration of high-performance characteristics on an economical carbon-based substrate creates sensors that are simultaneously affordable enough to be disposable yet stable enough to be reusable, effectively bridging the critical gap between sophisticated laboratory systems and scalable, field-deployable solid ISEs for environmental monitoring applications.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100367"},"PeriodicalIF":7.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling MOF-derived CuFe2O4 as a catalytic promoter of Ru(bpy)32⁺ ECL: A CRISPR/Cas13a-based strategy for miR-17 Detection","authors":"Yanli Sun ,&nbsp;Hongying Li ,&nbsp;Tao Wang ,&nbsp;Kai Zhang ,&nbsp;Xueliang Wang","doi":"10.1016/j.snr.2025.100406","DOIUrl":"10.1016/j.snr.2025.100406","url":null,"abstract":"<div><div>MicroRNA-17 (miR-17) is a critical biomarker linked to various cancers and diseases, underscoring the need for sensitive and selective detection methods. Herein, an ultra-sensitive electrochemiluminescent (ECL) sensor for miR-17 detection utilizing MOF-derived porous CuFe₂O₄ nanospheres and CRISPR/Cas13a trans-cleavage-triggered hybridization chain reaction (HCR) amplification was presented. The porous CuFe₂O₄ nanospheres possessed a high surface area and excellent catalytic activity, significantly enhancing ECL emission. To the best of our knowledge, this is the first report demonstrating that MOF-derived CuFe₂O₄ can effectively enhance the electrochemiluminescence of Ru(bpy)₃²⁺ for miR-17 detection. The CRISPR/Cas13a system provided miR-17 recognition and trans-cleavage activity, triggering HCR for signal amplification. Under optimized conditions, the biosensor exhibited a wide linear detection range from 20 aM to 5000 aM, with a detection limit of 9.23 aM. The system discriminated miR-17 from closely related miRNAs with negligible cross-reactivity, which demonstrated its outstanding selectivity. Practical applicability was confirmed by successful quantification of miR-17 in diluted human serum which achieved recovery rates between 95.2% and 104.8%. The results validated the biosensor’s potential for early disease diagnosis and clinical monitoring of miR-17-associated disorders.</div></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"10 ","pages":"Article 100406"},"PeriodicalIF":7.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145568657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}