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A 16-channel Si probe monolithically integrated with CMOS chips for neural recording 用于神经记录的与 CMOS 芯片单片集成的 16 通道硅探针
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-21 DOI: 10.1016/j.snr.2024.100206
Guang-Yang Gou , Changhua You , Pan Yao , Yu-Sen Guo , Tie-Zhu Liu , Zi-Xuan Song , Ben-Yuan He , MingHui Yin , Xuan Zhang , Chunxiu Liu , Jun Zhou , Xuan Sun , Chengyu Zhuang , Yuan-Dong Gu , Lei Yao , Ning Xue , Ming Zhao
{"title":"A 16-channel Si probe monolithically integrated with CMOS chips for neural recording","authors":"Guang-Yang Gou ,&nbsp;Changhua You ,&nbsp;Pan Yao ,&nbsp;Yu-Sen Guo ,&nbsp;Tie-Zhu Liu ,&nbsp;Zi-Xuan Song ,&nbsp;Ben-Yuan He ,&nbsp;MingHui Yin ,&nbsp;Xuan Zhang ,&nbsp;Chunxiu Liu ,&nbsp;Jun Zhou ,&nbsp;Xuan Sun ,&nbsp;Chengyu Zhuang ,&nbsp;Yuan-Dong Gu ,&nbsp;Lei Yao ,&nbsp;Ning Xue ,&nbsp;Ming Zhao","doi":"10.1016/j.snr.2024.100206","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100206","url":null,"abstract":"<div><p>Multi-channel neural electrodes as a crucial means are of great significance for information exchange between the brain and computers. Herein, we present a 16-channel Si-based active neural probe system that achieves a monolithic integration between the electrodes and circuits in a single probe, making it a standalone integrated electrophysiology recording system. The ASIC prepared on a base (<span><math><mrow><mn>2</mn><mspace></mspace><mo>×</mo><mspace></mspace><mn>2</mn><mspace></mspace><mi>m</mi><msup><mrow><mi>m</mi></mrow><mn>2</mn></msup></mrow></math></span>) is a 16-channel analog frontend (AFE) for neural recording, and each channel has a low-noise amplifier (LNA), a bandpass filter (BPF), a buffer and a current bias circuit. The 258 neural signal recording electrodes (<span><math><mrow><mn>22</mn><mspace></mspace><mo>×</mo><mspace></mspace><mn>24</mn><mspace></mspace><mi>μ</mi><msup><mrow><mi>m</mi></mrow><mn>2</mn></msup></mrow></math></span>) are densely packed on a 50 μm thick, 100 μm wide, and 3 mm long shank. The ASIC of neural probe, internal interconnecting wires are all implemented in commercial SMIC 0.18 μm CMOS technology. The neural probe system achieves a 3.6 μV<sub>rms</sub> input-referred noise (IRN) in a bandwidth of 1.1Hz-10 kHz, 70.8 μW power consumption, 0.0785 mm<sup>2</sup> area per channel, as well as an AFE gain of 58.1 dB Furthermore, the impedances of the Au electrodes can be obtained as 0.5–2.1 MΩ at a frequency of 1 kHz. The functionality of a 16-channel silicon-based neural probe is validated in an in-vivo experiment on lab rats.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100206"},"PeriodicalIF":6.5,"publicationDate":"2024-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000225/pdfft?md5=c1e13733680810a35fc396ccb92755a3&pid=1-s2.0-S2666053924000225-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141486208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Critical review of potential technologies for a wearable benzene sensor system 对可穿戴式苯传感器系统潜在技术的严格审查
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-20 DOI: 10.1016/j.snr.2024.100210
Tim Dunker, Alain Marc Ferber, Håkon Sagberg, Kari Anne Hestnes Bakke
{"title":"Critical review of potential technologies for a wearable benzene sensor system","authors":"Tim Dunker,&nbsp;Alain Marc Ferber,&nbsp;Håkon Sagberg,&nbsp;Kari Anne Hestnes Bakke","doi":"10.1016/j.snr.2024.100210","DOIUrl":"10.1016/j.snr.2024.100210","url":null,"abstract":"<div><p>We evaluate different methods to detect benzene at a parts-per-billion level regarding their potential to be used in a wearable sensor. Benzene is a carcinogenic molecule, regarded as a major health threat by the World Health Organization. A wearable sensor is necessary to detect leaks immediately, but it is challenging to achieve such low limits of detection and quantification, even with laboratory equipment. A wearable sensor must, in addition to good selectivity and sensitivity, meet stricter requirements of size, weight, temperature, repeatability, and power consumption. We conclude that the most promising techniques for a wearable sensor are either infrared photoacoustic spectroscopy near 14.8 μm, or a photoionization detector combined with one of three selective devices: micro-gas chromatography, cavitands, or catalytic filters (<span><math><msub><mtext>WO</mtext><mn>3</mn></msub></math></span>, for example). Ultraviolet photoacoustic spectroscopy may also be a suitable future technique for a wearable benzene sensor when efficient LEDs and lasers become available at many UV-C wavelengths.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100210"},"PeriodicalIF":6.5,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000262/pdfft?md5=383f024ef002140d9fed222c5d50c652&pid=1-s2.0-S2666053924000262-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142049432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In-situ spectroelectrochemical analysis: Irreversible deformation of cesium lead bromide Perovskite Quantum Dots in SiOx matrices 原位光谱电化学分析:氧化硅基质中的溴化铯铅 Perovskite 量子点的不可逆变形
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-20 DOI: 10.1016/j.snr.2024.100208
Hyeri Jeon , Hyeonyeong Jo , Sumi Seo , Soo Jeong Lee , Seog Joon Yoon , Donghoon Han
{"title":"In-situ spectroelectrochemical analysis: Irreversible deformation of cesium lead bromide Perovskite Quantum Dots in SiOx matrices","authors":"Hyeri Jeon ,&nbsp;Hyeonyeong Jo ,&nbsp;Sumi Seo ,&nbsp;Soo Jeong Lee ,&nbsp;Seog Joon Yoon ,&nbsp;Donghoon Han","doi":"10.1016/j.snr.2024.100208","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100208","url":null,"abstract":"<div><p>To practically utilized the organometallic lead halide perovskites to optoelectronic devices and photoelectrochemical cells, numerous efforts have been utilized to obtain the perovskites with low-energy process with coverage of various inorganic mediums to improve stability against humidity. By utilizing ligand-assisted reprecipitation process, under ambient condition at room temperature, the dimensionally confined perovskite quantum dots in silica matrices (PQD@SiO<sub>x</sub>) were obtained, and they were stable under several months under the ambient condition. To apply the PQD@SiO<sub>x</sub> to the photoelectrochemical cells by introducing direct contact between PQD@SiO<sub>x</sub> and electrolyte, the material/photophysical properties under electrochemical conditions are necessary to be studied. However, the role of silica coverage to the electrochemical behaviors of the PQD cores in the silica medium were not yet studied. In this work, under the electrochemical conditions, the oxidative and reductive behaviors of the PQD@SiO<sub>x</sub> were studied. Also, through <em>in-situ</em> spectroelectrochemical study, the electrochemically induced irreversible deformation process were tracked. The findings of this study could be used to understand role of silica coverage and develop the strategy to improve the protecting behavior of the silica for the PQD cores to utilize into the photoelectrochemical cells.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100208"},"PeriodicalIF":6.5,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000249/pdfft?md5=f3232432c75a6c1d6c97987820de091d&pid=1-s2.0-S2666053924000249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141483081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning 通过支持智能手机的数字 CRISPR 设备和深度学习,快速、便携地量化艾滋病毒 RNA
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-19 DOI: 10.1016/j.snr.2024.100212
Hoan T. Ngo , Patarajarin Akarapipad , Pei-Wei Lee , Joon Soo Park , Fan-En Chen , Alexander Y. Trick , Tza-Huei Wang , Kuangwen Hsieh
{"title":"Rapid and portable quantification of HIV RNA via a smartphone-enabled digital CRISPR device and deep learning","authors":"Hoan T. Ngo ,&nbsp;Patarajarin Akarapipad ,&nbsp;Pei-Wei Lee ,&nbsp;Joon Soo Park ,&nbsp;Fan-En Chen ,&nbsp;Alexander Y. Trick ,&nbsp;Tza-Huei Wang ,&nbsp;Kuangwen Hsieh","doi":"10.1016/j.snr.2024.100212","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100212","url":null,"abstract":"<div><p>For the 29.8 million people in the world living with HIV/AIDS and receiving antiretroviral therapy, it is crucial to monitor their HIV viral loads. To this end, rapid and portable diagnostic tools that can quantify HIV RNA are critically needed. We report herein a rapid and quantitative digital CRISPR-assisted HIV RNA detection assay that has been implemented within a portable smartphone-based device as a potential solution. Specifically, we first developed a fluorescence-based reverse transcription recombinase polymerase amplification (RT-RPA)-CRISPR assay that can efficiently detect HIV RNA at 42 °C. We then implemented this assay within a commercial stamp-sized digital chip, where RNA molecules were quantified as strongly fluorescent digital reaction wells. The isothermal reaction condition and the strong fluorescence in the digital chip simplified the design of thermal and optical modules, allowing us to engineer a palm-size device measuring 70 × 115 × 80 mm and weighing less than 0.6 kg. We also capitalized the smartphone by developing a custom app to control the device, perform the digital assay, and capture fluorescence images throughout the assay using the smartphone's camera. Moreover, we trained and verified a deep learning-based algorithm for analyzing fluorescence images and identifying positive digital reaction wells with high accuracy. Using our smartphone-enabled digital CRISPR device, we successfully detected as low as 75 copies of HIV RNA in just 15 min, showing its potential toward monitoring of HIV viral loads and aiding the global effort to combat the HIV/AIDS epidemic.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100212"},"PeriodicalIF":6.5,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000286/pdfft?md5=d6be4571c77e1353b94cc1b899a5b0f1&pid=1-s2.0-S2666053924000286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141483084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Highly sensitive and selective optical detection of Staphylococcus aureus using thiol functionalized monolayer tungsten disulfide grown by chemical vapor deposition 利用化学气相沉积法生长的硫醇功能化单层二硫化钨对金黄色葡萄球菌进行高灵敏度和选择性光学检测
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-18 DOI: 10.1016/j.snr.2024.100214
Abdul Kaium Mia , Swapnil Sinha , P.K. Giri
{"title":"Highly sensitive and selective optical detection of Staphylococcus aureus using thiol functionalized monolayer tungsten disulfide grown by chemical vapor deposition","authors":"Abdul Kaium Mia ,&nbsp;Swapnil Sinha ,&nbsp;P.K. Giri","doi":"10.1016/j.snr.2024.100214","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100214","url":null,"abstract":"<div><p>Monolayer tungsten disulfide (1L-WS<sub>2</sub>) exhibits excellent optical properties due to its direct bandgap. The extraordinary photoluminescence (PL) emission at room temperature from CVD-grown 1L-WS<sub>2</sub> was utilized for the first time here as a recognition tool for detecting <em>S. aureus</em> bacteria with high sensitivity and selectivity. The 1L-WS<sub>2</sub> possesses sulfur vacancy, which has been utilized for single-standard DNA (ssDNA) aptamer immobilization via the thiol functional group. The small-sized, highly selective ssDNA aptamers identify and selectively interact with targeted <em>S. aureus</em>, enabling selective detection. Interestingly, the PL emission of 1L-WS<sub>2</sub> is strongly influenced by external charge doping. The shape of the PL emission peak of 1L-WS<sub>2</sub> undergoes significant changes in the presence of targeted <em>S. aureus</em> as a result of charge transfer originating from selective interactions between ssDNA aptamer and <em>S. aureus,</em> while it remains unaffected for non-targeted <em>Escherichia coli</em>. The ratio of the integrated intensities of trion to neutral exciton peak was used as a calibration parameter for the quantification of <em>S. aureus</em> concentrations. The PL analysis of 1L-WS<sub>2</sub> with increasing concentration of <em>S. aureus</em> exhibits a linear response over 10<sup>2</sup> CFU/mL to 10<sup>7</sup> CFU/ml with a lower detection limit of 2.0 CFU/mL. The proposed sensing system can identify an unknown concentration of <em>S. aureus</em> in human urine with 78% accuracy at a concentration of 10<sup>5</sup> CFU/mL. These results demonstrate the potential future generation applications of monolayer transition metal dichalcogenides in the optical biosensing of pathogenic species using suitable receptors.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100214"},"PeriodicalIF":6.5,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000304/pdfft?md5=848f6df7623689629b7489d7ee5b0e06&pid=1-s2.0-S2666053924000304-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141438862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inflammatory biomarker detection in saliva samples by printed graphene immunosensors 利用印刷石墨烯免疫传感器检测唾液样本中的炎症生物标记物
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-15 DOI: 10.1016/j.snr.2024.100211
D. Vurro , L. Pasquardini , M. Borriello , R. Foresti , M. Barra , M. Sidoli , D. Pontiroli , L. Fornasini , L. Aversa , R. Verucchi , P. D'Angelo , G. Tarabella
{"title":"Inflammatory biomarker detection in saliva samples by printed graphene immunosensors","authors":"D. Vurro ,&nbsp;L. Pasquardini ,&nbsp;M. Borriello ,&nbsp;R. Foresti ,&nbsp;M. Barra ,&nbsp;M. Sidoli ,&nbsp;D. Pontiroli ,&nbsp;L. Fornasini ,&nbsp;L. Aversa ,&nbsp;R. Verucchi ,&nbsp;P. D'Angelo ,&nbsp;G. Tarabella","doi":"10.1016/j.snr.2024.100211","DOIUrl":"10.1016/j.snr.2024.100211","url":null,"abstract":"<div><p>Herein, we present the design and fabrication of a portable biochemical sensor based on the Screen Printed Electrode (SPE) concept and applied for detecting interleukin-6 (IL-6), a key player in the complex process of inflammation, in real human saliva. The sensing mechanism relies on the antigen-antibody binding between the IL-6 molecule and its antibody immobilized over a surface of a Thermally Exfoliated Graphene Oxide (TEGO) layer. TEGO, deposited by Aerosol Jet Printing (AJP), provides advantages in terms of a time/cost consuming<del>fast</del>, easy and efficient biofunctionalization. The biosensor shows a dynamic range comprising IL-6 concentrations falling within the normal IL-6 levels in saliva. An extensive analysis of device performance, focused on the assessment of the sensor Limit of Detection (LoD) by two modes (i.e. from the lin-log calibration curve and from blank measurements), provides a best value of about 1 × 10<sup>−2</sup> pg/ml of IL-6 in saliva. Our work aims at providing a contribution toward applications in real environment, going beyond a proof of concept or prototyping at lab scale. Hence, the characterization of the sensor was finalized to find a reliable device-to-device reproducibility and calibration through the introduction of a measurement protocol based on comparative measurements between saliva samples without (blank) and with IL-6 spiked in it, in place of the <em>standard addition method</em> used in daily laboratory practice. Device-to-device reproducibility has been accordingly tested by acquiring multiple experimental points along the calibration curve using different individual devices for each point.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100211"},"PeriodicalIF":6.5,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000274/pdfft?md5=11adee908fe24b8c5ea7c622778ff555&pid=1-s2.0-S2666053924000274-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141413916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Applications of electrochemical impedance spectroscopy in disease diagnosis—A review 疾病诊断中的电化学阻抗光谱应用 - 综述
IF 6.5
Sensors and Actuators Reports Pub Date : 2024-06-12 DOI: 10.1016/j.snr.2024.100205
José A. Ribeiro , Pedro A.S. Jorge
{"title":"Applications of electrochemical impedance spectroscopy in disease diagnosis—A review","authors":"José A. Ribeiro ,&nbsp;Pedro A.S. Jorge","doi":"10.1016/j.snr.2024.100205","DOIUrl":"10.1016/j.snr.2024.100205","url":null,"abstract":"<div><p>Electrochemical impedance spectroscopy (EIS) is a reliable technique for gathering information about electrochemical process occurring at the electrode surface and investigating properties of materials. Furthermore, EIS technique can be a very versatile and valuable tool in analytical assays for detection and quantification of several chemically and biologically relevant (bio)molecules. The first part of this Review (<em>Introduction</em>) provides brief insights into (i) theoretical aspects of EIS, (ii) the instrumentation required to perform the EIS studies and (iii) the most relevant representations of impedance experimental data (such as Nyquist and Bode plots). In the end of this section, (iv) theoretical aspects regarding the fitting of the Randles circuit to experimental data are addressed, not only to obtain information about electrochemical processes but also to illustrate its utility for analytical purposes. The second part of the Review (<em>Impedimetric Detection of Disease Biomarkers</em>) focuses on the applications of EIS in the biomedical field, particularly as analytical technique in electrochemical sensors and biosensors for screening disease biomarkers. In the last section (<em>Conclusions and Perspectives</em>), we discuss main achievements of EIS technique in analytical assays and provide some perspectives, challenges and future applications in the biomedical field.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100205"},"PeriodicalIF":6.5,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000213/pdfft?md5=f068273f787566f7142495848e036e2e&pid=1-s2.0-S2666053924000213-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141401091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrasensitive detection of interleukin-6 enabled by three-dimensional spatial amplification 利用三维空间放大技术实现白细胞介素-6 的超灵敏检测
IF 5.9
Sensors and Actuators Reports Pub Date : 2024-06-10 DOI: 10.1016/j.snr.2024.100213
Masaki Yamaguchi , Vivek Shetty
{"title":"Ultrasensitive detection of interleukin-6 enabled by three-dimensional spatial amplification","authors":"Masaki Yamaguchi ,&nbsp;Vivek Shetty","doi":"10.1016/j.snr.2024.100213","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100213","url":null,"abstract":"<div><p>Accurately measuring interleukin-6 (IL-6) levels is crucial in both clinical medicine and research due to its role in various physiological and pathological processes. We present proof-of-concept of a novel three-dimensional (3D) spatial amplification method for developing highly sensitive and miniaturized IL-6 biosensors. We designed an IL-6 immunosensor platform utilizing a unique 3D microfluidic structure fabricated with femtosecond-pulsed laser processing. This design features a porous transducer element with over 4500 microcavities, thereby increasing the reaction area by 13-fold compared to traditional 2D designs while maintaining transparency. By combining this 3D structure with a competitive antigen-antibody reaction, the sensor achieved an exceptional limit of detection of 0.27 pg/mL for IL-6 in human blood samples. Additionally, we demonstrated effective control of liquid flow within the porous element using a reduction of pressure and centrifugation speeds. This 3D spatial amplification method offers a promising approach for developing highly sensitive and compact IL-6 biosensors. Such miniaturized sensors hold potential for point-of-care testing devices, enabling convenient and timely IL-6 analysis.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100213"},"PeriodicalIF":5.9,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000298/pdfft?md5=6276c30b19805284de285d9877568356&pid=1-s2.0-S2666053924000298-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141323998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Paper-based immunosensor integrated with bioinspired Cu-polydopamine nanozyme for voltammetric detection of CA-15-3 tumor marker 集成了生物启发铜-多巴胺纳米酶的纸基免疫传感器,用于伏安法检测 CA-15-3 肿瘤标志物
IF 5.9
Sensors and Actuators Reports Pub Date : 2024-06-10 DOI: 10.1016/j.snr.2024.100209
Mohammad Ali Farzin, Seyed Morteza Naghib
{"title":"Paper-based immunosensor integrated with bioinspired Cu-polydopamine nanozyme for voltammetric detection of CA-15-3 tumor marker","authors":"Mohammad Ali Farzin,&nbsp;Seyed Morteza Naghib","doi":"10.1016/j.snr.2024.100209","DOIUrl":"https://doi.org/10.1016/j.snr.2024.100209","url":null,"abstract":"<div><p>This manuscript describes the fabrication and characterization of a highly conductive paper-based nanoplatform for immobliztion of anti-CA 15-3 antibodies. For this purpose, cellulosic filter paper (FP) was first coated with Cu nanosponges (CuNSs) through ion beam sputtering deposition (IBSD) method and then covered with Cu-doped polydopamine nanospheres (Cu-PDA). Polydopamine doped with copper exhibited laccase (multi-copper oxidase)-mimicking properties. Bioinspired by the structure of the active site and the electron transfer mechanism of laccase, Cu-PDA nanozyme catalyzed the oxidation of hydroquinone (HQ) signal probe. In this design, CA-15-3, a prognostic biomarker in the breast cancer, was chosen as a model target. To the best of our knowledge, this is the only paper on the design of an electrochemical biosensor based on dense and uniform CuNSs produced by IBSD. More importantly, Cu-PDA is biocompatible and biodegradable, which makes it a potential competitor in the point-of-care (POC) biosensing applications. Thus, this paper-based sensing strategy can be used in designing cost-effective flexible chips consisting of electrodes printed on paper for in-situ and real-time monitoring biomarkers under clinical conditions. In addition to these outstanding features, the LOD (1.3 mU mL 1) and LOQ (4.3 mU mL 1) of the Ab/HQ/Cu-PDA/CuNSs/FP-based immunosensor was significantly lower than the clinically relevant cut-off level (30 U mL 1). In the practical applications, one of the key superiorities of this immuosensor is the wide DLR (5 mU mL 1–280 U mL 1) which covers the ultimate value of healthy and patient individuals.</p></div>","PeriodicalId":426,"journal":{"name":"Sensors and Actuators Reports","volume":"8 ","pages":"Article 100209"},"PeriodicalIF":5.9,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666053924000250/pdfft?md5=04eb6669534ae720eaeb2e18fb583fa8&pid=1-s2.0-S2666053924000250-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141323999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fabrication of humidity monitoring sensor using porous silicon nitride structures for alkaline conditions 利用多孔氮化硅结构制造碱性条件下的湿度监测传感器
IF 5.9
Sensors and Actuators Reports Pub Date : 2024-06-06 DOI: 10.1016/j.snr.2024.100203
Soobin Park , Inseong Hwang , Jae Chan Park , Tae Joo Park , Han-Seung Lee , Sang Yeon Lee , Hyun-Min Yang , Bongyoung Yoo
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