SERS nanotags for ultrasensitive and dual biomarker detection of breast cancer

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports Pub Date : 2025-06-01 DOI:10.1016/j.snr.2025.100344

Huiying Hou , Junqi Zhao , Zhengliang Zuo , Li Song , Xiao Xia Han , Jin Yang , Baofeng Xu , Bing Zhao

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引用次数: 0

Abstract

Sensitive and simultaneous detection of breast cancer 1 (BRCA1) and breast cancer 2 (BRCA2) is of significance for the early diagnosis of breast cancers, but it still challenges conventional methods. Herein, we developed a novel surface-enhanced Raman spectroscopy (SERS) based method for quantification of breast cancer biomarkers BRCA1 and BRCA2. Highly uniform SERS-active substrate is obtained by self-assembly of silver nanoparticles on a polystyrene template. Gold core silver shell (Au@Ag) nanoparticles embedded with Raman probes were synthesized and used as SERS nanotags. The target genes BRCA1 and BRCA2 were detected through the capture DNA modified on the Ag chip and the probe DNA attached on the SERS nanotags. Dual detection is achieved by changing Raman probes, capture DNA and probe DNA sequences. This method exhibits high selectivity and sensitivity with a wide dynamic linear range (0.001−1000 nM). Moreover, its applicability to the detection of BRCA1 and BRCA2 in cell lysates has been proved, showing its potential application in breast cancer diagnosis and prognosis.

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SERS纳米标签用于乳腺癌的超灵敏和双生物标志物检测

同时灵敏检测乳腺癌1 （BRCA1）和乳腺癌2 （BRCA2）对乳腺癌的早期诊断具有重要意义，但仍对传统方法提出了挑战。在此，我们开发了一种新的基于表面增强拉曼光谱（SERS）的方法来定量乳腺癌生物标志物BRCA1和BRCA2。通过在聚苯乙烯模板上自组装银纳米粒子获得了高度均匀的sers活性底物。合成了嵌入拉曼探针的金核银壳纳米粒子（Au@Ag）作为SERS纳米标签。通过在Ag芯片上修饰的捕获DNA和附着在SERS纳米标签上的探针DNA检测靶基因BRCA1和BRCA2。双重检测是通过改变拉曼探针，捕获DNA和探针DNA序列实现的。该方法具有较高的选择性和灵敏度，动态线性范围宽（0.001 ~ 1000 nM）。此外，其对细胞裂解物中BRCA1和BRCA2检测的适用性已得到证实，显示了其在乳腺癌诊断和预后方面的潜在应用。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.