Fast and sensitive virus detection using a plasmonic sensor that integrates electrokinetically assisted sampling and surface-enhanced Raman scattering

IF 6.5 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Sensors and Actuators Reports Pub Date : 2024-12-26 DOI:10.1016/j.snr.2024.100273

Shamim Azimi , Maryam Moridsadat , Bhavin Shastri , Bruce W. Banfield , Carlos Escobedo , Aristides Docoslis

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Abstract

We present a novel plasmonic biosensing method for on-chip detection on viral particles featuring a microelectrode platform that integrates accelerated sampling of virus with surface-enhanced Raman scattering (SERS). We show experimentally that our approach can produce spectacular results owing to the unique incorporation of two key features: (1) Concentration amplification of virus on the SERS-active substrate; (2) Local plasmonic activity enhancement due to the targeted superimposition of silver nanoparticles on the captured virus sites. When tested for the detection of the M13 bacteriophage our “sandwich” assay yielded excellent reproducibility (signal variation <6 %) and a very low limit of detection (1.13 × 10² pfu/ml). Compared with the performance of our standard SERS substrates, SERS signals stronger by at least one order of magnitude are typically achieved. In addition to experimental results, our work also includes finite element (COMSOL Multiphysics) and finite-difference time-domain (FDTD) simulations that provide insights into the mechanisms of concentration amplification and plasmonic activity enhancement, respectively.

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使用集成了电动辅助采样和表面增强拉曼散射的等离子体传感器进行快速灵敏的病毒检测

我们提出了一种新的等离子体生物传感方法，用于芯片上检测病毒颗粒，该方法采用微电极平台，集成了病毒加速采样和表面增强拉曼散射（SERS）。我们通过实验证明，我们的方法可以产生惊人的结果，因为我们独特地结合了两个关键特征：(1)病毒在sers活性底物上的浓度扩增；(2)局部等离子体活性增强是由于银纳米粒子在捕获的病毒位点上的靶向叠加。当用于检测M13噬菌体时，我们的“三明治”试验具有出色的再现性（信号变异<； 6%）和极低的检测限（1.13 × 102 pfu/ml）。与我们的标准SERS基板的性能相比，通常可以实现至少一个数量级的SERS信号增强。除了实验结果，我们的工作还包括有限元（COMSOL Multiphysics）和时域有限差分（FDTD）模拟，分别为浓度放大和等离子体活性增强的机制提供了见解。

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来源期刊

Sensors and Actuators Reports Multiple-

CiteScore

9.60

自引率

0.00%

发文量

审稿时长

49 days

期刊介绍： Sensors and Actuators Reports is a peer-reviewed open access journal launched out from the Sensors and Actuators journal family. Sensors and Actuators Reports is dedicated to publishing new and original works in the field of all type of sensors and actuators, including bio-, chemical-, physical-, and nano- sensors and actuators, which demonstrates significant progress beyond the current state of the art. The journal regularly publishes original research papers, reviews, and short communications. For research papers and short communications, the journal aims to publish the new and original work supported by experimental results and as such purely theoretical works are not accepted.