Results in Chemistry最新文献

{"title":"Characterisation and purification of Commiphora gileadensis extracts with Antiproliferative activities on breast Cancer cell lines","authors":"Abdullah F. Aldairi ,&nbsp;Sarah A. Alharbi ,&nbsp;Moodi S. Alharbi ,&nbsp;Ahmad Alghamdi ,&nbsp;Anmar Khan ,&nbsp;Mazen Ghaith ,&nbsp;Hani Alnadwi ,&nbsp;Abdullah Alshehri ,&nbsp;Asma Alsubaihi ,&nbsp;Ayman Alhazmi","doi":"10.1016/j.rechem.2025.102216","DOIUrl":"10.1016/j.rechem.2025.102216","url":null,"abstract":"<div><div>Breast cancer is a common form of cancer that affects women all over the world. Cancer patients have relied on surgery and radiotherapy, chemically derived medications for treatment, which result in several unpleasant side effects. To overcome these issues, medicinal plants would be used as modern pharmaceutical agents to treat various diseases, such as cancer. The study aims to evaluate the antiproliferative activities of <em>Commiphora gileadensis (L.) C.Chr</em> extracts against breast cancer cell lines MCF-7 and MDA-MB-231. Methods: <em>Commiphora gileadensis (L.) C.Chr</em> was harvested and cleaned; chemical extraction was applied using acetone and methanol, then cell viability was measured. The active extract was characterised via untargeted lipidomics analysis. Results: The acetone extract of <em>Commiphora gileadensis (L.) C.Chr</em> showed growth inhibition against MCF-7 and MDA-MB-231 cell lines with IC50 (57.32 μg/mL) and (49.94 μg/mL), respectively. The methanolic extract showed antiproliferative activities against the MCF-7 cell line with IC50 (306.7 μg/mL) and the IC50 (273.6 μg/mL) for MDA-MB-231. Untargeted lipidomic analysis revealed the presence of various lipid classes in each extract. In the acetone extract, the most abundant lipid compounds are hexosylceramide (39.1 %) and ceramide (18.6 %). On the other hand, the lipidomics analysis of the methanolic extract showed the composition consists primarily of ceramide (69 %). In conclusion, the acetone and methanol extracts of <em>Commiphora gileadensis (L.) C.Chr</em> exhibited cytotoxic properties against breast cancer cell lines.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102216"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in silico and in vitro approach for understanding CDK2 expression pattern and prognostic implications in breast cancer","authors":"Fatima Manhas,&nbsp;Shazia Sofi,&nbsp;Asma Jan,&nbsp;Nusrat Jan,&nbsp;Manzoor Ahmad Mir","doi":"10.1016/j.rechem.2025.102207","DOIUrl":"10.1016/j.rechem.2025.102207","url":null,"abstract":"<div><h3>Background</h3><div>CDK2 (Cyclin-Dependent Kinase 2) is a Ser/Thr kinase that regulates cell cycle progression by promoting the G1/S transition and initiating DNA synthesis through its interaction with cyclins E and A. Unchecked cell growth, DNA damage, and protein deterioration are caused by an abnormally high level of CDK2. When it does so, it responds in excess, which triggers unchecked cell proliferation, which is what happens in breast cancer</div></div><div><h3>Objective</h3><div>The study's primary objective is to explore CDK2's expression profile, functional role, and prognostic importance in the development of breast cancer</div></div><div><h3>Methods</h3><div>In this study, we evaluated CDK2's expression profile, prognostic implications and interactions with other proteins to better understand how CDK2 influences the etiology of breast cancer. A range of computational techniques, including UALCAN, TIMER2.0, GEPIA, STRING, DISCO, ENRICHR, and Docking, were utilized</div></div><div><h3>Results</h3><div>Breast cancer patients had highly regulated CDK2 levels. Utilizing cytohubba, the most significant 10 hub genes of the web were identified. The regulation of heterochromatin formation and cellular response to nitric acid are substantially enriched in the KEGG pathway analysis and gene ontology GO. Association with the p53 pathway was revealed by the KEGG pathway investigation. Adapalene strongly bound to protein CDK2 in a 3D schematic, with the lowest binding energy being −10.87 kcal/mol</div></div><div><h3>Conclusion</h3><div>Collectively, the study shows that CDK2 promotes tumor growth and that blocking it in addition to conventional therapies will significantly enhance the medical outcomes of breast cancer patients.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102207"},"PeriodicalIF":2.5,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of nano hydroxyapatite loaded gellan gum nanocomposite scaffold for the regeneration of bone tissue affected by osteosarcoma","authors":"Huaidong Wang ,&nbsp;Tao Bai ,&nbsp;Mahani Yusoff ,&nbsp;Nur Ain Atisya C.M. Khairuddin ,&nbsp;Alina Irwana Muhamad A'srai ,&nbsp;Mohd Hasmizam Razali","doi":"10.1016/j.rechem.2025.102208","DOIUrl":"10.1016/j.rechem.2025.102208","url":null,"abstract":"<div><div>Advanced biomaterials play a critical role in promoting bone tissue engineering and regenerative medicine applications. This study aimed to fabricate a biomimetic scaffold incorporating nano-hydroxyapatite (nHA) and gellan gum (GG) for bone tissue repair. Nanocomposite scaffold loaded with nHA nanoparticles within a gellan gum matrix (nHA@GG) was successfully produced using a freeze-drying technique. Characterization by Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), and Scanning Electron Microscopy (SEM) corroborated the successful fabrication of the nHA@GG nanocomposite scaffold. XRD and FTIR analyses verified the phase composition and chemical bonding characteristics of hydroxyapatite, aligning with its successful formation within the scaffold. These findings highlight the potential of the nHA@GG nanocomposite scaffold to facilitate and accelerate apatite growth in in-vitro models. This might be attributed to the presence of nHA, which can act as a nucleation site for secondary apatite layer formation. The nHA@GG scaffold demonstrated efficient encapsulation and drug loading, with 35.48 ± 0.84 % encapsulation and 25.75 ± 0.75 % drug loading of sodium alendronate. The cumulative drug release of 38 % was achieved within 4 days. Furthermore, biocompatibility studies utilizing MC3T3 cells demonstrated significant cell proliferation (&gt;100 %) and high cell viability (&gt;90 %) on the nHA@GG scaffold. These findings, coupled with the favorable physicochemical properties and non-toxic nature of the nHA@GG scaffold, highlight its promising potential as a biomaterial for bone tissue regeneration.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102208"},"PeriodicalIF":2.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid removal of anionic dyes from water, using poly (diallyldimethylammonium chloride) and branched polyethyleneimine functionalized cellulose extracted from Echinops bannaticus leaves","authors":"Raoudha Soury ,&nbsp;Mahjoub Jabli ,&nbsp;Ahmed Al Otaibi","doi":"10.1016/j.rechem.2025.102209","DOIUrl":"10.1016/j.rechem.2025.102209","url":null,"abstract":"<div><div>In the current work, we report the extraction and chemical functionalization of cellulose from <em>Echinops bannaticus</em> (<em>E. bannaticus</em>) leaves. Poly (diallyldimethylammonium chloride) (PDDAC) and branched polyethyleneimine (BPEI) were used as amine reagents. The prepared cellulosic substrates were characterized using several analytical techniques including SEM, FT-IR, XRD, and TGA/DTG techniques. FT-IR data proved the chemical interaction between hydroxyl groups of cellulose and amine groups of PDDAC and BPEI. XRD pattern of the extracted cellulose showed main peaks situated at 14.4°, 22.7° and 34.3°, corresponding to (110), (200) and (040) lattice planes of crystalline cellulose. The functionalization of cellulose with PDDAC and BPEI did not affect the crystallinity. After chemical modification, the cellulosic fibers appeared physically deformed with the existence of some surface alteration. The variation in thermal decomposition values indicated that many non-cellulosic components were removed during alkalization and bleaching processes. After thermal degradation, the residual mass was found to be more important in the case of raw <em>E. bannaticus</em> fiber (16.59 %) compared to the extracted cellulose (5.31 %). The analyzed cellulosic materials were investigated for their ability to adsorb an anionic Acid Blue 25 (AB25) dye from water. The adsorption results showed high adsorption capacities of the aminated cellulosic materials. Under optimum conditions (mass adsorbent = 0.01 g, pH = 5, time = 60 min., and <em>T</em> = 22 °C), the adsorption capacities of the extracted cellulose-HPEI (3 %), extracted cellulose-PDDAC (5 %), and unmodified extracted cellulose were 109 mg/g, 64 mg/g, and 24 mg/g, respectively. The fitting of the adsorption data with theoretical kinetic and isotherm equations indicated that the mechanism complied well with pseudo-second-order and both Langmuir and Freundlich models. Overall, we reported an efficient adsorbent derived from naturally abundant and cost-effectives bio-resources for eliminating hazardous anionic dyes from polluted waters.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102209"},"PeriodicalIF":2.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile green diversity-oriented synthesis, molecular docking, and cytotoxicity evaluation of quinoline -triazole appended peptidomimetics as inhibitors of human breast cancer cell line MCF-7","authors":"K. Ramya ,&nbsp;Seena Chakko ,&nbsp;V.S. Shinu ,&nbsp;Davis Varghese ,&nbsp;Ron Joy Pullukkara ,&nbsp;S. Arun","doi":"10.1016/j.rechem.2025.102172","DOIUrl":"10.1016/j.rechem.2025.102172","url":null,"abstract":"<div><div>The synthesis of quinoline-based drugs has significant interest from researchers due to their broad spectrum of biological activities. In this context, a concise approach for the synthesis of quinoline-functionalized hybrid peptidomimetics is described. The peptidomimetics are constructed based on a recombinant approach via Iqbal multicomponent coupling strategy and click chemistry and were linked via copper (I) catalysed [3 + 2] azide-alkyne cycloaddition. The peptidomimetics showed exceptional inhibitory properties for CDK2 protein which is responsible for many malignancies and also showed remarkable cytotoxicity against human breast cancer cell line MCF-7. The IC₅₀ value (8 μM) and binding affinity (−10.2 Kcal/mol) found for <strong>4b</strong> against MCF-7 cells are hopeful for the development of potential anticancer drugs based on these new scaffolds.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102172"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143706526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemical properties of bioactive bioplastics based on cellulose from coffee and cocoa by-products","authors":"Robin Oblitas ,&nbsp;Luz Quispe-Sanchez ,&nbsp;Grobert Guadalupe ,&nbsp;Elgar Hernandez Diaz ,&nbsp;Segundo Oliva ,&nbsp;Jorge R. Diaz-Valderrama ,&nbsp;Ives Yoplac ,&nbsp;Cristina Valencia-Sullca ,&nbsp;Segundo G. Chavez","doi":"10.1016/j.rechem.2025.102201","DOIUrl":"10.1016/j.rechem.2025.102201","url":null,"abstract":"<div><div>Plastic pollution has become a critical environmental issue due to the extensive use of petroleum-derived plastics, which persist in ecosystems and pose significant challenges to sustainability. This study evaluated the physicochemical properties of bioactive bioplastics made from cellulose extracted from coffee by-products (spent coffee grounds) and cocoa (pods), which were proposed as a sustainable alternative to mitigate the environmental impact of petroleum-derived plastics. The formulation of the bioplastics involved varying proportions of cellulose (1, 3, and 6 %) in combination with bioactive cacao extracts (0.05 % and 0.15 % <em>w</em>/<em>v</em>). A comprehensive array of physicochemical properties was analyzed, encompassing parameters such as color change, whiteness index, water activity, solubility, transparency, flexibility, antioxidant capacity, and biodegradation. The results demonstrated that the bioplastics with 3 % cocoa pod cellulose (CMC) and 0.05 % bioactive extract exhibited the highest whiteness index (86.9) and the most excellent flexibility (32.24 N). In contrast, formulations comprising 6 % CMC exhibited reduced water activity (0.49). Bioplastics containing 1 % coffee cellulose (PC) and 0.15 % bioactive extract demonstrated a high antioxidant capacity (0.38 mgTE/L). Furthermore, it was observed that an increase in cellulose content led to a decrease in the biodegradation rate, with the control achieving 98 % degradation within 35 days. This study provides fundamental information for designing bioplastics with specific properties, thereby promoting the development of more sustainable and environmentally friendly packaging technologies.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102201"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of N3-substituted-quinazoline-2,4(1H,3H)-diones via CuI-catalyzed coupling of 2-iodobenzamides with potassium cyanate","authors":"Ali Nasirpour ,&nbsp;Zarrin Ghasemi ,&nbsp;Seyed Abolfazl Hosseini-Yazdi ,&nbsp;Morgane Poupon ,&nbsp;Cinthia Antunes Correa","doi":"10.1016/j.rechem.2025.102204","DOIUrl":"10.1016/j.rechem.2025.102204","url":null,"abstract":"<div><div>Herein, we disclosed the synthesis of <em>N</em>3-substituted-quinazoline-2,4(1<em>H</em>,3<em>H</em>)-diones via the consecutive copper-catalyzed coupling/annulation of 2-iodobenzamides and KOCN. This transformation is characterized by the in situ-generation of arylisocyante through C(<em>sp</em>^<em>2</em>)-N(<em>sp</em>^<em>2</em>) bond formation, which enriches the library of preparation quinazolindiones. Moreover, it is worth noting that replacing the iodo group with bromo also produced a comparable yield of the corresponding quinazolinedione derived under the present conditions.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102204"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, crystal structure, DFT calculations, and molecular docking of N-(4-bromophenyl)pyridine-2-carboxamide palladium(II) complexes","authors":"Pinky N. Mjwara ,&nbsp;Eric O. Akintemi ,&nbsp;Siphamandla Sithebe ,&nbsp;Hadley S. Clayton ,&nbsp;Tshephiso R. Papo","doi":"10.1016/j.rechem.2025.102194","DOIUrl":"10.1016/j.rechem.2025.102194","url":null,"abstract":"<div><div>A novel palladium(II) complex, ([N-(4-bromophenyl)pyridine-2-carboxamidato]-(chloro)-(pyridine)‑palladium, <strong>C2</strong>) was synthesized from an aqueous solution of potassium tetrachloropalladate, pyridine and the <em>N</em>-<!--> <!-->(4-<!--> <!-->bromophenyl)<!--> <!-->pyridine-2-carboxamide ligand. Single crystal X-ray crystallography analysis reveals that the asymmetric units of C₁₇H₁₅BrClN₃O₂Pd belong to the orthorhombic crystal system with the Pbca space group. The experimentally determined structure of <strong>C2</strong> was examined and compared to a previously synthesized analogous bis[N-(4-bromophenyl)pyridine-2-carboxamidato]palladium complex (<strong>C1</strong>) by density functional theory (DFT) and molecular docking studies. DFT b3lyp/3-21g method gave reasonably low optimization energies and band energy value of ∼3.2 eV for both complexes, suggesting they are chemically reactive. Molecular docking simulations through the docking scores show that <strong>C2</strong> (−121.2 and −119.2 kcal/mol) is more potent than <strong>C1</strong> (−73.1 and −106.6 kcal/mol) against prostate and cervical cancers, while <strong>C1</strong> (−78.5 and −108.3 kcal/mol) is more potent than <strong>C2</strong> (−88.5 and −116.5 kcal/mol) against skin and breast cancer. Some key interacting residues in the binding site of prostate cancer protein include Trp227, Phe306, Phe311, and Tyr317. The cervical cancer protein includes Tyr15, Asp127, Val18 and Glu12. For skin cancer protein, they are Arg223, Glu226, Leu227 and Ile224; and for breast cancer protein are Cys2085, Glu2052, Phe2048, and Gln2082. Generally, the Pd(II) complexes show promising anticancer potential and could potentially be a more effective chemotherapeutic than the standard carboplatin drug.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102194"},"PeriodicalIF":2.5,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon nanotubes in brain targeted drug delivery: A comprehensive review","authors":"Sourav Das ,&nbsp;Sukanta Roy ,&nbsp;Subhas Chandra Dinda ,&nbsp;Anirbandeep Bose ,&nbsp;Chinmaya Mahapatra ,&nbsp;Biswajit Basu ,&nbsp;Bhupendra Prajapati","doi":"10.1016/j.rechem.2025.102206","DOIUrl":"10.1016/j.rechem.2025.102206","url":null,"abstract":"<div><div>The treatment of brain diseases remains a significant challenge in modern medicine, primarily due to the restrictive nature of the blood-brain barrier (BBB), which limits drug delivery. Carbon nanotubes (CNTs) have emerged as a promising strategy for overcoming this obstacle, providing an innovative platform for targeted drug delivery to the brain. This review presents a detailed analysis of CNTs as drug carriers, with a particular emphasis on their application in brain tumor treatment. Their unique physicochemical properties, including a high surface area, tunable surface chemistry, and exceptional drug-loading capacity, make them ideal candidates for encapsulating and delivering therapeutic agents with precision. Functionalization with targeting ligands further enhances the specificity of CNTs, enabling drugs to reach brain tumors more effectively while minimizing off-target effects. The review examines advancements in CNT-based drug delivery systems, particularly their role in transporting chemotherapy drugs, nucleic acid-based therapies, and other small molecules across the BBB. It also highlights their potential to improve therapeutic efficacy while reducing systemic toxicity. Furthermore, the challenges and opportunities associated with CNT-based drug delivery, such as safety concerns, regulatory barriers, and clinical translation, are explored. The discussion extends to the development of multifunctional CNT platforms that combine imaging agents with therapeutic payloads, allowing for real-time tumor monitoring and treatment. Ultimately, this review provides insights into the potential of CNTs as carriers for brain-targeted drug delivery, offering a pathway toward more effective and less invasive brain tumor therapies. Understanding current advancements and addressing existing challenges will be crucial for realizing the full potential of CNT-based drug delivery in neuro-oncology.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102206"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Monte Carlo simulation and quantitative microbial risk approach to investigate seasonal variation of airborne particulate matter and bioaerosols in medical waste management department and wastewater treatment plant of Iranian hospitals","authors":"Mahbubeh Tangestani ,&nbsp;Ahmad Jonidi Jafari ,&nbsp;Majid Kermani ,&nbsp;Roshanak Rezaei Kalantary ,&nbsp;Hossein Arfaeinia","doi":"10.1016/j.rechem.2025.102196","DOIUrl":"10.1016/j.rechem.2025.102196","url":null,"abstract":"<div><div>This study addresses a significant knowledge gap by conducting a thorough analysis of pollutants in hospital environments and utilizing advanced risk assessment models to evaluate their health impacts on workers for the first time. The study aimed to assess the concentration of particulate matter (PM₁_–_2.5, PM_0.5_–₁, PM_0.25_–_0.5, and PM_˂0.25) and bioaerosols (bacteria and fungi) in the air around waste disposal departments and wastewater treatment plants of selected hospitals in Bushehr city, Iran, during different seasons. A probabilistic risk assessment using Monte Carlo simulation and the Disability-Adjusted Life Years (DALYs) model was employed to evaluate the health risks of PM and bioaerosols on workers. The study collected 32 PM samples and 132 bioaerosol samples using a four-stage impactor and a single-stage Anderson sampler, respectively. The results showed that the highest concentration of PM was found in PM₁_–_2.5, measuring 119.4 μg/m³, while bacteria and fungi concentrations were 617 and 756 CFU/m³, respectively. Additionally, larger PM sizes were more prevalent, and bacterial levels exceeded those of fungi in the wards. Waste disposal sections in hospitals exhibited higher pollution levels of PM, bacteria, and fungi compared to wastewater treatment plants, especially during summer. Bioaerosol levels exceeded existing standards in the surrounding air of these departments Risk values surpassed acceptable thresholds, indicating significant health concerns. Sensitivity analysis revealed that concentration (C) and exposure duration (ED) parameters had the most significant impact on worker health assessment.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102196"},"PeriodicalIF":2.5,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143687401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}