Results in Chemistry最新文献

{"title":"Advanced strategies for screening and identifying RNA-targeted small molecules: Bridging therapeutic potential and innovation","authors":"Zukela Ruzi ,&nbsp;Daxiong Han ,&nbsp;Kailibinuer Aierken","doi":"10.1016/j.rechem.2025.102305","DOIUrl":"10.1016/j.rechem.2025.102305","url":null,"abstract":"<div><div>The development of RNA-targeted small molecules has emerged as a promising and rapidly expanding field in drug discovery, offering new opportunities to address a wide range of diseases beyond protein-targeting therapies. RNA molecules, including messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs), play fundamental roles in gene regulation, protein synthesis, and cellular stress responses. However, their dynamic nature and structural complexity have historically posed challenges in developing small molecules with high affinity and selectivity. In recent years, significant advancements in screening methodologies, such as high-throughput screening (HTS), small molecule microarrays (SMMs), and DNA-encoded libraries (DELs), have facilitated the identification of RNA-binding small molecules with enhanced specificity. Complementary approaches, including structure-based drug design (SBDD) and fragment-based drug discovery (FBDD), have provided critical insights into RNA-ligand interactions, enabling rational optimization of candidate molecules. The integration of emerging technologies, such as RNA sequencing and chemical crosslinking, has further enabled transcriptome-wide mapping of interactions and predictive modeling of binding affinity. This review presents a comprehensive analysis of the methodologies employed in the discovery of RNA-targeted small molecules, with a focus on their applications in bacterial riboswitches, viral RNA elements, and disease-associated RNAs, such as miRNA-21 and MALAT1. Despite recent progress, challenges remain, including the lack of well-defined RNA binding pockets and potential off-target effects. Strategies to overcome these obstacles are explored, highlighting the potential of RNA-targeted small molecules to address unmet therapeutic needs. By integrating cutting-edge technologies and multidisciplinary approaches, this review provides insights into current advances and future directions in RNA-targeted drug discovery.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102305"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in multifunctional nanomaterials for synergistic photocatalytic and adsorptive water treatment processes","authors":"Athba Sabhan Khalaf ,&nbsp;Zainab Y. Shnain ,&nbsp;Alaa Dhari Jawad Al-Bayati ,&nbsp;Hasan Sh. Majdi ,&nbsp;Mohammad F. Abid ,&nbsp;Ahmed Abdalhussein Bilal ,&nbsp;Adnan.A. Alsalim ,&nbsp;Nour Hamid Abdrahman ,&nbsp;Aswar A. Alwasiti","doi":"10.1016/j.rechem.2025.102292","DOIUrl":"10.1016/j.rechem.2025.102292","url":null,"abstract":"<div><div>Wastewater generated from different anthropogenic activities often poses health risks to both human and aquatic lives which necessitate the development of advanced remediation technologies using multifunctional nanocomposites. There is an increasing interest in the application of multifunctional nanocomposites for wastewater treatment due to their tendency to be used in combined photocatalytic and adsorption process. The multifunctional nanocomposites offer synergistic effects which provide opportunities for efficient capture of the contaminants and subsequently degrading them under various environmental conditions. The recent advances in the applications of multifunctional nanocomposites include the design of photocatalysts that could be applied under visible light irradiation, surface modified adsorbents, and heterojunction nanomaterials. Multifunctional nanocomposites have displayed noteworthy performance in the removal of organic contaminants such as dyes, pharmaceuticals residues, phenols as well as heavy metals with enhanced stability, reusability and scalability. Key advancements in the application of the multifunctional nanocomposite, and the various mechanism in the adsorption and photocatalytic process have been highlighted in this review. The review presented a future perspective with an emphasis on the necessity of cost-effectiveness and environmentally sustainable nanomaterials to ensure sustainable wastewater treatment technologies.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102292"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-friendly synthesis of magnetic Pd/NiFe₂O₄/chitosan nanobiocatalyst for enhanced degradation of Congo red dye under sunlight irradiation","authors":"Mohammad Panahimehr ,&nbsp;Mehdi Hosseini ,&nbsp;Ayyub Mojaddami ,&nbsp;Shahnaz Karamipour","doi":"10.1016/j.rechem.2025.102300","DOIUrl":"10.1016/j.rechem.2025.102300","url":null,"abstract":"<div><div>In this research, the magnetic nanobio photocatalyst Pd/NiFe₂O₄/chitosan was synthesized using a green synthesis method. The characterization of this photocatalyst was carried out through several techniques including FT-IR, XRD, VSM, TEM, FESEM, and EDS. FESEM images confirm that the particle morphology is rice-like and uniform. Also, the TEM image confirms the incorporation of palladium in the chitosan matrix with an average particle size of 18 nm. Optical properties investigation using the Tauc diagram showed that the band gap of the photocatalyst is 2.52 eV, placing it within the visible light range. The performance of this photocatalyst for the degradation of the organic dye Congo red under sunlight irradiation was evaluated and the results showed that this photocatalyst was able to degrade the dye with an efficiency of 94.9 % within 30 min. Another outstanding feature of this photocatalyst was its recyclability with magnetic separation and reusability. After five consecutive cycles, the photocatalyst was easily separated by an external magnet and maintained its performance without significant structural changes. The results of this research demonstrate that Pd/NiFe₂O₄/chitosan acts as a highly efficient and stable photocatalyst, capable of degrading organic pollutants under ambient conditions. The integration of chitosan biopolymer into the nanophotocatalyst's design represents a key strength of this study, serving dual roles in facilitating eco-friendly synthesis and enhancing environmental compatibility. Furthermore, the incorporation of palladium effectively suppresses electron-hole recombination while narrowing the band gap, thereby improving photocatalytic efficiency.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102300"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DFT study of 1,4-diazabicyclo[2.2.2]octane (DABCO) based ionic liquids: Effect of alkyl chain and anion types","authors":"Azim Soltanabadi,&nbsp;Zahra Fakhri","doi":"10.1016/j.rechem.2025.102285","DOIUrl":"10.1016/j.rechem.2025.102285","url":null,"abstract":"<div><div>This study examines how 1,4-diazabicyclo[2.2.2]octane (DABCO) interacts with different anions and alkyl chain lengths ([C_nDABCO⁺][X¯], where <em>n</em> = 0, 1, 2, or 3, and X = Cl¯, Br¯, PF₆¯, or BF₄¯). We used Density Functional Theory (DFT) with the 6–311++G(d,p) basis set for geometry optimization and electronic property calculations. Atoms in Molecules (AIM) and Natural Bond Orbital (NBO) analyses were applied to understand bonding and charge distribution. By incorporating AIM and NBO analyses, we gained insight into the nature of bonding and charge distribution within the complexes. Larger anions like PF₆¯, or BF₄¯exhibit higher electronic stability than smaller halide anions. A key finding of this work is the substantial role of hydrogen bonding, as revealed by AIM analysis, particularly in systems involving PF₆¯, or BF₄¯. These hydrogen bonds are shown to enhance the structural stability of the ILs and contribute to their distinctive physicochemical behaviors.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102285"},"PeriodicalIF":2.5,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homocoupling reaction of p-tolylboronic acid with palladium-hydroxyapatite composite from chicken eggshell as the catalyst","authors":"Dea Putri Novianti ,&nbsp;Muhamad Rashifari ,&nbsp;Muhammad R. Ramadhan ,&nbsp;Iis Intan Widiyowati ,&nbsp;Puteri Khansa Salsabila ,&nbsp;Ardanari Wahyu Waardhani ,&nbsp;Muhamad Diki Permana ,&nbsp;Atiek Rostika Noviyanti ,&nbsp;Yudha P. Budiman","doi":"10.1016/j.rechem.2025.102297","DOIUrl":"10.1016/j.rechem.2025.102297","url":null,"abstract":"<div><div>Biaryl compounds, consist of two aromatic rings linked by a single bond, are crucial in the synthesis of antiviral drugs, antifungal agents, functional materials, and agrochemicals. Traditionally, these compounds are synthesized through cross-coupling reactions using two different types of aryl reagents. However, homocoupling reactions have emerged as a valuable alternative, enabling the production of symmetric biaryls from a single aryl substrate. This study explores the development of a heterogeneous palladium-hydroxyapatite (Pd-HAP) catalyst for the homocoupling of arylboronic acids. Hydroxyapatite, known for its hydroxyl-rich surface, high porosity, and strong acid-base sites, was selected as a cost-effective catalyst support. The research focused on optimizing solvent and temperature to maximize biaryl yields. Under optimal conditions ethanol as the solvent at 50 °C for 24 h, an 88 % yield of biaryl products was achieved. The recycled catalyst maintained a yield of 42 % after five reuses. These findings contribute to more sustainable and cost-effective approaches for biaryl synthesis.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102297"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theoretical study of the sequence-dependent distribution of Na+ ions around DNA","authors":"Hui-ting Xu ,&nbsp;Xiao-wei Jiang ,&nbsp;Yu-jie Song ,&nbsp;Bin Gu","doi":"10.1016/j.rechem.2025.102290","DOIUrl":"10.1016/j.rechem.2025.102290","url":null,"abstract":"<div><div>Sodium ions play a crucial role in diverse biological processes. This study focuses on the sequence-dependent distribution of sodium ions around DNA, utilizing the bsc0 force field to simulate and accurately replicate the conformational changes observed in Raman spectroscopy experiments, and provides an explanation for the phenomenon of bimodal distribution of pAC sequences. Molecular dynamics simulations revealed that Na⁺ ions exhibiting unique distributions in pAA, pGA, and pGG oligomers. These ions progressively shift from the phosphate group and minor groove towards the G-tract region of the major groove. Significantly, the pGC sequence exhibits a transition in Na⁺ ion distribution from the major to minor grooves. The overall binding of sodium ions to the pAC sequence is weak. The pAT sequence reduces the ionic affinity in the groove, and a small portion is distributed near the ATA·TAT base order in the minor groove. The study determined a 75 % neutralization rate across DNA oligonucleotides, independent of sequence. Analyses of weak interactions revealed stable G·C and A·T hydrogen bonds alongside diminished C-H···O bonds, and a reduction in base pair stacking interaction with sequence variation. Sodium ions bind to DNA with over 95 % electrostatic interaction energy, the pAA oligomer exhibits the lowest interaction energy. The distribution of water molecules around DNA is less affected by sequence. The study provides a deep atomic-level understanding of the behavior of sodium ions around DNA.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102290"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143878911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the potential of functionalized FeS2 composites as efficient photocatalysts for improved removal of cationic and anionic dyes","authors":"Vahid Mohammadbeigi,&nbsp;Ebrahim Allahkarami,&nbsp;Amirreza Azadmehr","doi":"10.1016/j.rechem.2025.102281","DOIUrl":"10.1016/j.rechem.2025.102281","url":null,"abstract":"<div><div>This study investigates the enhancement of pyrite (FeS₂) through functionalization with silanol (SiO₂) and silanol-amino (SiO₂-NH₂) groups to optimize its adsorption and photocatalytic performance for removing cationic (methylene blue, MB) and anionic (methyl orange, MO) dyes. Functionalized core-shell composites, Pyrite@SiO₂ and Pyrite@SiO₂-NH₂, were synthesized via a sol-gel process using tetraethyl orthosilicate (TEOS) and 3-aminopropyl triethoxysilane (APTS). Comprehensive characterization through XRF, XRD, FT-IR, TEM, UV–vis DRS, and BET analysis revealed spherical nanoparticles with smooth surfaces and uniform SiO₂/SiO₂-NH₂ coatings (200–300 nm thick). Functionalization significantly increased the specific surface area from 0.64 m²/g (raw pyrite) to 3.21 m²/g, enhancing pollutant interaction sites. Adsorption dominated over photocatalytic degradation under both UV and dark conditions, with maximum capacities of 36.5 mg/g (MO) and 34.0 mg/g (MB) at 298 K. Kinetic studies aligned with the pseudo-second-order model, while mass transfer analysis identified intraparticle diffusion as the primary mechanism. Although UV irradiation marginally improved dye removal, surface charge modification via SiO₂-NH₂ groups notably enhanced MO adsorption through electrostatic interactions. These findings underscore pyrite's potential as a cost-effective, efficient adsorbent for wastewater treatment, with surface functionalization offering a strategic avenue to tailor affinity for target pollutants, prioritizing adsorption over photocatalytic approaches.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102281"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of ubiquinone Q10 (Co Q10) from natural tobacco leaves","authors":"Venkata Manikya Kumar Ajjarapu","doi":"10.1016/j.rechem.2025.102279","DOIUrl":"10.1016/j.rechem.2025.102279","url":null,"abstract":"<div><div>The present research work is aimed to develop a methodology for the synthesis of Ubiquinone Q₁₀ (CoQ₁₀) from the natural tobacco leaves (<em>Nicotiana tabacum</em> L). Solanesol (<em>C</em>_<em>45</em><em>H</em>_<em>74</em><em>O</em>), a product isolated from the natural tobacco leaves and a valuable source for synthesizing metabolically active quinones and other drugs has been considered as the raw material for the synthesis of Ubiquinone. The characteristics of Ubiquinone as an antioxidant, essential for human life and the critical role of Ubiquinone in slowing down the ageing process and its uses in the treatment of heart diseases, cancer and ulcers are also presented. Bromination of solanesol with PBr₃ results in the formation of solanesyl bromide with an extremely high yield (98 %) and on further treatment with reagents such as ethylacetate followed by the hydrolysis and decarboxylation results in the formation of a ketone with an appreciable yield of 72 % has also been explained. The chemical pathways suggested for the synthesis of decaprenol and isodecaprenol with significant and appreciable yield are also summarized in the research paper. Formation of Ubiquinone has been identified by TLC and further purified by employing a C_18, 3.9 mm × 150 mm reverse phase column chromatography with methanol and n-hexane (90:10 <em>v</em>/v). Solvent crystallization in alcohol at <img>20⁰C has been carried out and the isolated ubiquinone is analyzed by HPLC for purity and spectroscopic techniques such as UV, ¹H and ¹³C NMR, FT- IR, and ESI-MS have been employed for the structural elucidation and the details are discussed at length in the research paper.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102279"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143873640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing desalination performance and antifouling properties of thin film composite nanofiltration membrane via polyacrylic acid incorporating","authors":"Ibtissem Ounifi ,&nbsp;Ali Boubakri ,&nbsp;Sarra Elgharbi ,&nbsp;Ali Rebhi ,&nbsp;Olfa Bechambi ,&nbsp;Amor Hafiane","doi":"10.1016/j.rechem.2025.102282","DOIUrl":"10.1016/j.rechem.2025.102282","url":null,"abstract":"<div><div>This study reports the development of novel polyamide thin-film composite (TFC) nanofiltration (NF) membranes with enhanced antifouling properties. The membranes were fabricated via interfacial polymerization between piperazine (PIP) and trimesoyl chloride (TMC) on a cellulose acetate (CA) ultrafiltration support membrane. A key innovation involved the in-situ incorporation of polyacrylic acid (PAA) into the polyamide selective layer by dispersing it within the aqueous PIP monomer solution. The synthesized membranes underwent comprehensive characterization to elucidate their surface morphology, topography, and hydrophilicity. Additionally, the permeability and separation properties of membranes were evaluated. The results revealed that incorporating PAA into the TFC-NF membranes significantly increased surface roughness and hydrophilicity compared to reference membranes without PAA. The effect of PAA concentration on water permeability was investigated. Interestingly, water permeability increased from 8.5 LMH/bar to 17.9 LMH/bar with increasing PAA concentration from 0 wt% to 0.7 wt%. The order of salt rejection followed the trend: Na₂SO₄ &gt; MgSO₄ &gt; MgCl₂ &gt; NaCl, which can be attributed to the introduction of negative charges of the modified membranes. Notably, the TFC membrane containing 0.5 wt% PAA exhibited the highest salt rejection, achieving 99.65 % rejection for Na₂SO₄ and 70.12 % rejection for NaCl. The antifouling properties of the TFC membranes were evaluated by monitoring their flux decline during bovine serum albumin (BSA) protein solution filtration. The incorporation of PAA into the polyamide layer of the TFC-NF membranes demonstrably improved their fouling resistance due to the increased negative charge, which repels negatively charged foulants like BSA.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102282"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on the application of the HPLC/PDA method to enhance the quality standards of Folium nelumbinis based on the bioactive nuciferine and quercetin 3-O-glucuronide","authors":"Sil Thanh Nguyen,&nbsp;Ngan Hong Tran,&nbsp;Yen Phuong Ngo,&nbsp;Vi Lan Le Truong,&nbsp;Tuyet Anh Thi Phan,&nbsp;Hien Trung Do,&nbsp;Tho Vinh Minh Chau Do","doi":"10.1016/j.rechem.2025.102291","DOIUrl":"10.1016/j.rechem.2025.102291","url":null,"abstract":"<div><div>This study enhances quality control standards for <em>Folium Nelumbinis</em>, a medicinal herb in the Vietnamese and Chinese Pharmacopeias. Current standards are insufficient for accurately quantifying key bioactive compounds, nuciferine and quercetin 3-<em>O</em>-glucuronide, which are vital for the plant's pharmacological effects. The quality control standards include sensory characteristics, microscopic features, powder examination, physicochemical properties, limits for heavy metals, and qualitative. Additionally, simultaneous quantification of nuciferine and quercetin 3-<em>O</em>-glucuronide was achieved using HPLC on a GL Inert Sustain C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase, consisting of acetonitrile (A) and 0.1 % triethylamine in water at pH 6.5 adjusted with 30 % acetic acid (B), was used with a flow rate of 1 mL/min and a detection wavelength of 270 nm. A 200 mg <em>Folium nelumbinis</em> powder was extracted with 6.0 mL of methanol and 1 % hydrochloric acid (6:4, <em>v</em>/v) via ultrasonication at 40 kHz and 500 W for 7 min, followed by centrifugation at 6000 rpm for 7 min (repeated three times). Physico-chemical standards studied are moisture content (8.74 %, <em>w</em>/w), impurities (0.74 %, w/w), fragmentation ratio (1.07 %, w/w), and ash content (6.50 %, w/w). The linearity of the calibration curve ranged from 12 to 48 μg/mL for nuciferine and 4–16 μg/mL for quercetin 3-<em>O</em>-glucuronide, and the square of the regression coefficient (r²) was 0.9990 and 0.9954, respectively. The limit of detection and quantification values were determined as 0.45 μg/mL and 1.50 μg/mL for nuciferine and 0.15 μg/mL and 0.50 μg/mL for quercetin 3-<em>O</em>-glucuronide. This research successfully establishes high-quality control standards for Lotus leaves in accordance with WHO guidelines and the Vietnamese Pharmacopoeia V. The validated HPLC method ensures precise and reliable quality control, supporting research consistency and practical herbal medicine application.</div></div>","PeriodicalId":420,"journal":{"name":"Results in Chemistry","volume":"15 ","pages":"Article 102291"},"PeriodicalIF":2.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}