Plant Gene最新文献_第7页

Regulation of sodium-inducible genes and efficient use of sodium ions determine the tolerance strategies of Cakile maritima and Brassica tournefortii in natural habitats 钠诱导基因的调控和钠离子的有效利用决定了天然生境中海洋毛蚶（cile martima）和热带芸苔（Brassica tournefortii）的耐盐策略

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-04-03 DOI: 10.1016/j.plgene.2025.100512

Abdel Hamid A. Khedr , Mamdouh S. Serag , Haneen A. Abdulsamad , Reham M. Nada

{"title":"Regulation of sodium-inducible genes and efficient use of sodium ions determine the tolerance strategies of Cakile maritima and Brassica tournefortii in natural habitats","authors":"Abdel Hamid A. Khedr , Mamdouh S. Serag , Haneen A. Abdulsamad , Reham M. Nada","doi":"10.1016/j.plgene.2025.100512","DOIUrl":"10.1016/j.plgene.2025.100512","url":null,"abstract":"<div><div>One of the potential routes for improving the stress tolerance of crops is discovering the tolerance mechanisms of halophytes. <em>Cakile maritima</em> and <em>Brassica tournefortii</em> were collected from four sites with different salinity levels. Two populations for each species were collected from each site. The populations of <em>C. maritima</em> had different phenotypic traits, especially at high salinity levels. Meanwhile, the populations of <em>B. tournefortii</em> had approximately similar phenotypic traits at all sites. The present study aimed to compare the tolerance strategies used by <em>C. maritima</em> and <em>B. tournefortii</em> by examining the responses of different populations of each species to high salinity levels in their natural habitats. The evolutionary relationship among the populations of each species was recorded. Growth performance, pigment concentration, Rubisco protein content, ion concentration and regulation of salt-inducible genes were evaluated. At high salinity levels, the biomass of most <em>C. maritima</em> populations increased, but the reverse was true for <em>B. tournefortii</em> populations. The acclimation of <em>B. tournefortii</em> to salt stress depends on Na<sup>+</sup> extrusion mechanisms. Meanwhile, <em>C. maritima</em> acclimated by a regulated and controlled ion uptake, regulated salt-inducible genes and efficient use of Na<sup>+</sup> in osmotic adjustment. Hierarchical analysis revealed that the expression pattern of Na<sup>+</sup>-inducible genes was not only species-dependent but also organ-dependent. The expression pattern did not correspond to the profiles of promoter regulatory motifs of the examined genes. The study concluded that the tolerance mechanisms are not static among halophytes, but they are dependent on the species and even on the population of a species.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100512"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel 合成六倍体小麦多样性面板中根生长角、种子根数和叶面积的全基因组关联

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-04-11 DOI: 10.1016/j.plgene.2025.100507

Maria Khalid , Zoya Khalid , Zubair Ahmed , Lee Hickey , Awais Rasheed , Alvina Gul , Rabia Amir

{"title":"Genome-wide association of root growth angle, seminal root numbers, and leaf area in the synthetic hexaploid wheat diversity panel","authors":"Maria Khalid , Zoya Khalid , Zubair Ahmed , Lee Hickey , Awais Rasheed , Alvina Gul , Rabia Amir","doi":"10.1016/j.plgene.2025.100507","DOIUrl":"10.1016/j.plgene.2025.100507","url":null,"abstract":"<div><div>A lack of understanding about the variations in root number, root angle, and leaf area contributes to inability of wheat withstand drought stress during seedling stage. This discrepancy hinders the development of drought-tolerant wheat varieties. We aimed to evaluate germplasm at seedling stage to find association between markers and traits. We characterized a collection of synthetic hexaploid wheat for root angle, seminal root numbers, and early vigor and identified loci associated with these traits using a 50 K SNP array and genotyping-by-sequencing (GBS) platforms. In total, 39 marker-trait nucleotides (QTNs) were associated with LA, RA, and RN distributed over 10 wheat chromosomes. Out of these, 11 QTNs were associated with LA, 15 QTNs were associated with RA, and 13 QTNs were associated with RN.LA showed a negative correlation with RN and a poor correlation with RA. The phenotypic values of synthetic hexaploid wheat accessions with a higher frequency of favorable alleles have been found to increase. Therefore, prioritizing the use of these synthetic wheat varieties in wheat breeding programs can be beneficial for improving root system architecture (RSA) and early vigor. These preliminary findings can be useful for pre-breeding efforts aimed at enhancing wheat adaptability to drought stress conditions.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100507"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development 艾蒿HD-Zip转录因子家族的全基因组鉴定及AaHDZ64在毛状体发育中的功能分析

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-06-27 DOI: 10.1016/j.plgene.2025.100529

Zhanhu Cui , Chunyan Miao , Heyang Shang , Qian Zhao , Shujiao Li , Zhongyi Zhang , Yuqing Wang , Xianzhang Huang

{"title":"Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development","authors":"Zhanhu Cui , Chunyan Miao , Heyang Shang , Qian Zhao , Shujiao Li , Zhongyi Zhang , Yuqing Wang , Xianzhang Huang","doi":"10.1016/j.plgene.2025.100529","DOIUrl":"10.1016/j.plgene.2025.100529","url":null,"abstract":"<div><div>The homeodomain-leucine zipper (HD-Zip) family of transcription factor is a unique and vital gene family in plants, involved in various processes including organ development, stress responses, and regulation of secondary metabolite biosynthesis. This study aimed to investigate the role of HD-Zip family transcription factors in the development of <em>Artemisia argyi</em> trichomes. By utilizing the whole genome data of <em>A. argyi</em>, we identified 69 AaHDZ transcription factors and systematically characterized their structural and physicochemical properties. The predicted amino acid sequence lengths range from 188 to 854 residues, with relative molecular masses of 22.2–94.6 kDa, theoretical isoelectric points of 4.65–9.31, and lipid solubility indices of 52.08–89.14. All of these proteins were hydrophilic. The results of subcellular localization predictions indicated that most AaHDZ proteins are localized in the nucleus, while a few are located in the cytoplasm and chloroplasts. Based on the classification of the HD-Zip family in <em>Arabidopsis thaliana</em>, the 69 AaHDZ proteins were divided into four subfamilies, with similar gene structures observed within each subfamily. Heterologous overexpression of <em>AaHDZ64</em> gene significantly increased trichome density in <em>A. thaliana</em>. The results confirmed that <em>AaHDZ64</em> is a positive regulatory factor for the growth and development of trichomes. This study provides a theoretical foundation for understanding the molecular regulatory mechanisms underlying trichome development in <em>A. argyi</em> leaves and offers insights for the precision breeding of <em>A. argyi</em>.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100529"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The calmodulin-binding transcriptional activator transcription factor family in foxtail millet (Setaria italica L.): Molecular characterization, codon bias, and evolutionary trajectory 谷子（Setaria italica L.）钙调素结合转录激活因子家族：分子特征、密码子偏好和进化轨迹

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-05-29 DOI: 10.1016/j.plgene.2025.100522

Huilong Chen , Kexin Ji , Yun Bai , Yuxian Li , Ying Liu , Fang Liu , Yutong Cui , Weina Ge , Zhenyi Wang

{"title":"The calmodulin-binding transcriptional activator transcription factor family in foxtail millet (Setaria italica L.): Molecular characterization, codon bias, and evolutionary trajectory","authors":"Huilong Chen , Kexin Ji , Yun Bai , Yuxian Li , Ying Liu , Fang Liu , Yutong Cui , Weina Ge , Zhenyi Wang","doi":"10.1016/j.plgene.2025.100522","DOIUrl":"10.1016/j.plgene.2025.100522","url":null,"abstract":"<div><div>Calmodulin-binding transcriptional activator (CAMTA) is a calmodulin-binding transcription factor, which plays an important role in calcium/calmodulin transduction signaling pathway. Foxtail millet (<em>Setaria italica</em> L.) is an economically important C4 model crop, but the genome-wide identification and molecular evolution of the CAMTA family is not yet available. In this study, seven CAMTA genes were identified from the foxtail millet genome via bioinformatics methods. They were overall similar in structure but differed, and all showed tissue expression specificity. Regarding molecular evolution, codon bias contributed to the evolution of the CAMTA family, and interestingly, base mutation was not the main factor affecting their codon bias, and may also be affected by factors such as natural selection and other factors. Multiple traces indicated that polyploidization events primarily led to the expansion of the plant CAMTA family, with overall similar but differentiated expansion histories in different species, and that <em>Selaginella moellendorfii</em> possesses the most paralogous genes being the most distinctive. More intriguingly, we found the opposite quantitative evolutionary history of terrestrial plants to that of the algal CAMTA family. Therefore, we firstly analyzed the molecular properties of the CAMTA transcription factor family in foxtail millet and explored its molecular evolutionary trajectory, and constructed a flow of evolutionary trajectories from monospecies transition to the plant kingdom for reference.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100522"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Priming-a state of alert against environmental stress challenges in plants 报春花--植物应对环境压力挑战的警戒状态

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-04-06 DOI: 10.1016/j.plgene.2025.100513

Divya Chaudhary , Himanshi Aggarwal , Banpreet Kaur , Himani Agarwal , Arghyabrata Das , Ajay Kumar , Vaibhav Mishra , Prashant Kumar Singh , Naveen Chandra Joshi

{"title":"Priming-a state of alert against environmental stress challenges in plants","authors":"Divya Chaudhary , Himanshi Aggarwal , Banpreet Kaur , Himani Agarwal , Arghyabrata Das , Ajay Kumar , Vaibhav Mishra , Prashant Kumar Singh , Naveen Chandra Joshi","doi":"10.1016/j.plgene.2025.100513","DOIUrl":"10.1016/j.plgene.2025.100513","url":null,"abstract":"<div><div>The changing climatic conditions have led many plants to restrict their growth and suppress their yield. In their struggle for survival, plants employ unique strategies that help them grow under extreme weather conditions and challenging biotic interactions. Plants can enhance their tolerance through a key process called priming, which strengthens their immune system's response to stimuli from pathogens, beneficial microbes, chemicals, and abiotic cues. Priming provides long-term stress resistance to plants and is based on a rapid and robust defence response to environmental stress conditions. A milder form of stress or chemicals, such as sodium selenite, melatonin, β-aminobutyricacid, polyamines, calcium, zinc, hydrogen peroxide, hydrogen sulfide and sodium hydrosulphide, are used to preactivate plant defence mechanisms. These chemicals play crucial role in stabilizing plant growth by empowering plants to activate their own defence mechanisms and combat unforeseen stressors. This review primarily explores the role of priming phenomena and the mechanisms that enable plants to withstand stressful environmental conditions in their natural habitat.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100513"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural and functional insights into Jasmonate ZIM-domain 2 (GhJAZ2) interactions in cotton fiber development via Jasmonic acid signaling 通过茉莉酸信号传导，茉莉酸zim -结构域2 （GhJAZ2）在棉纤维发育中的相互作用的结构和功能研究

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-06-28 DOI: 10.1016/j.plgene.2025.100528

Muhammad Sulyman Saleem , Sultan Habibullah Khan , Zunaira Afzal Naveed

{"title":"Structural and functional insights into Jasmonate ZIM-domain 2 (GhJAZ2) interactions in cotton fiber development via Jasmonic acid signaling","authors":"Muhammad Sulyman Saleem , Sultan Habibullah Khan , Zunaira Afzal Naveed","doi":"10.1016/j.plgene.2025.100528","DOIUrl":"10.1016/j.plgene.2025.100528","url":null,"abstract":"<div><div>Cotton is one of the world's most vital fiber crops, and enhancing its fiber quality continues to be a major priority for the textile sector. The protein GhJAZ2, belonging to the Jasmonate ZIM-domain (JAZ) family, functions as a transcriptional repressor that influences fiber initiation by interacting with proteins such as GhMYB25-like, GhWD40, GhMYC2, GhGL1, and GhJI1, all of which participate in jasmonic acid (JA) signaling pathways. Despite their known involvement, detailed insights into their interactions and precise three-dimensional structures remain elusive.</div><div>In this research, we aimed to characterize the functional roles of GhJAZ2 and its interacting partners, and to model their structural interactions computationally. We utilized homology-based modeling to construct 3D models and applied HADDOCK software for protein-protein docking to accurately pinpoint interaction sites and assess binding affinities. Our findings indicated that the ZIM domain of GhJAZ2 serves as the central interaction region. Specifically, key residues within the TIFY motif, particularly ILE126 and TYR128, play a significant role in binding GhWD40, GhMYC2, GhGL1, and GhJI1 proteins. These results underscore the critical role of the TIFY motif in enabling GhJAZ2 to act as a repressor during fiber development.</div><div>This study provides valuable new insights into the molecular processes involved in JA-driven fiber development in cotton, identifying potential genetic targets that could be manipulated to significantly improve fiber quality.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100528"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide identification of aldehyde dhydrogenase (ALDH) family genes in pepper (Capsicum annuum) reveals involvement of CaALDH7B1 in heat and drought stress tolerance 辣椒（Capsicum annuum）醛氢酶（ALDH）家族基因的全基因组鉴定揭示了CaALDH7B1参与辣椒耐热和干旱胁迫耐受

IF 2.2

Plant Gene Pub Date : 2025-09-01 Epub Date: 2025-05-30 DOI: 10.1016/j.plgene.2025.100524

Bo Yang , Yifen Shang , Lang Wen , Yijia Cui , Zixing Li , Yuan Cheng , Chaochao Liu

{"title":"Genome-wide identification of aldehyde dhydrogenase (ALDH) family genes in pepper (Capsicum annuum) reveals involvement of CaALDH7B1 in heat and drought stress tolerance","authors":"Bo Yang , Yifen Shang , Lang Wen , Yijia Cui , Zixing Li , Yuan Cheng , Chaochao Liu","doi":"10.1016/j.plgene.2025.100524","DOIUrl":"10.1016/j.plgene.2025.100524","url":null,"abstract":"<div><div>Abiotic stresses such as heat and drought lead to oxidative damage in plants by inducing excessive accumulation of reactive oxygen species (ROS). Aldehyde dehydrogenases (ALDHs), which detoxify reactive aldehydes, play critical roles in stress responses, but their functions in pepper (<em>Capsicum annuum</em>) remain largely unexplored. In this study, 28 CaALDH genes were identified and categorized into nine families. Phylogenetic and synteny analyses revealed strong evolutionary conservation, highlighting CaALDH2B4 and CaALDH7B1 as key members. Expression profiling showed distinct tissue-specific patterns and robust induction under heat and drought stress. Functional analysis via virus-induced gene silencing (VIGS) confirmed that CaALDH7B1 enhances stress tolerance by limiting ROS accumulation, promoting antioxidant enzyme activity (SOD, CAT, APX, POD), and maintaining NADPH/NADP<sup>+</sup> homeostasis. Comparative genomics and structural modeling further revealed that CaALDH7B1 is evolutionarily conserved, with critical NADP<sup>+</sup>-binding residues retained across plant species. These findings underscore the pivotal role of CaALDH7B1 in oxidative stress regulation and provide new insights into the functional evolution of the ALDH gene family in pepper.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"43 ","pages":"Article 100524"},"PeriodicalIF":2.2,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144194646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome-wide characterization of the anthocyanin O-methyltransferases in the cultivated potato 栽培马铃薯花青素o -甲基转移酶的全基因组特征

IF 2.2

Plant Gene Pub Date : 2025-06-01 Epub Date: 2025-04-03 DOI: 10.1016/j.plgene.2025.100506

Annalisa Staiti , Carmine Fruggiero , Domenico Carputo , Vincenzo D’Amelia , Nunzio D’Agostino

{"title":"Genome-wide characterization of the anthocyanin O-methyltransferases in the cultivated potato","authors":"Annalisa Staiti , Carmine Fruggiero , Domenico Carputo , Vincenzo D’Amelia , Nunzio D’Agostino","doi":"10.1016/j.plgene.2025.100506","DOIUrl":"10.1016/j.plgene.2025.100506","url":null,"abstract":"<div><div><em>O</em>-methyltransferases (OMTs) are group of enzymes involved in the methylation of various secondary metabolites, including anthocyanins. This secondary modification, together with hydroxylation and glycosylation, affects the chromatic properties, stability and reactivity of these pigments. Meanwhile, no detailed identification or genome-wide analysis of the OMT gene family members in potato (<em>Solanum tuberosum</em>) has been reported. We conducted a genome-wide identification and characterization of potato OMTs, identifying 65 OMT family members. These were characterized based on gene structure, evolutionary relationships, and promoter motifs. Analysis of available gene expression profiles from public databases revealed expression patterns indicative of tissue- and temporal-specificity Finally, we further elucidated expression differences among four selected OMT-encoding genes using potato cell cultures, which represent a smart and gainful alternative for anthocyanin production and deep knowledge about these post-biosynthetic modifications.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100506"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding the evolution of dumbbell stomata: Insights from the developmental genes of sedges and grasses 解码哑铃气孔的进化：来自莎草和禾草发育基因的见解

IF 2.2

Plant Gene Pub Date : 2025-06-01 Epub Date: 2025-02-28 DOI: 10.1016/j.plgene.2025.100494

Alison P.A. Menezes , Emilio Petrone-Mendoza , James W. Clark , Salvatore Cozzolino

{"title":"Decoding the evolution of dumbbell stomata: Insights from the developmental genes of sedges and grasses","authors":"Alison P.A. Menezes , Emilio Petrone-Mendoza , James W. Clark , Salvatore Cozzolino","doi":"10.1016/j.plgene.2025.100494","DOIUrl":"10.1016/j.plgene.2025.100494","url":null,"abstract":"<div><div>Stomatal morphology is a defining trait among plant lineages. Grasses (Poaceae) have distinctive dumbbell-shaped stomata that enhance water-use efficiency compared to the more common kidney-shaped stomata. In the closely related sedges (Cyperaceae) dumbbell-like stomata can be found. Dumbbell-like stomata in sedges share morphological features with grasses, suggesting potential conservation or convergence of developmental pathways. To investigate the evolution of dumbbell and dumbbell-like stomata, we analyzed genomic and transcriptomic data from 29 sedge species and five grass species. Using their predicted proteomes, we identified orthologues involved in stomatal development and reconstructed their phylogenetic histories. Among the 16 gene families analyzed, <em>EPFL9, YODA, SCR</em>, and <em>SHR</em> were expanded in grasses but not in sedges. <em>POLAR, SPCH</em>, and <em>ABI</em> were expanded in both lineages, seven families were conserved in both, <em>BASL</em> is not present in both, and, in the <em>ICE1/SCRM2</em> family, <em>SCRM2</em> was lost while <em>ICE1</em> was duplicated in sedges. Gene family expansion in grasses occurred primarily in genes involved in early stages of stomatal development, while the non-duplicated or independently expanded genes shared by sedges and grasses contribute to the development of the two lateral subsidiary cells as well as the guard cells. The gene conservation and independent expansion suggest shared regulatory networks underlying the stomata morphology typical of the order Poales. This study serves as an evolutionary guide for testing functional proteins underlying paracytic dumbbell and dumbbell-like stomata development.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100494"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143550263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a meiotic atlas and chromosomal mapping of abundant genome elements in the orphan crop golden thistle (Scolymus hispanicus L.) 孤儿作物金蓟（Scolymus hispanicus L.）减数分裂图谱的建立及基因组丰富元素的染色体定位

IF 2.2

Plant Gene Pub Date : 2025-06-01 Epub Date: 2025-02-10 DOI: 10.1016/j.plgene.2025.100493

Ahmet L. Tek, Sevim D. Kara Öztürk, Hümeyra Yıldız Akkamış, Elif Sena Köksal, Emir Can Kaya

{"title":"Development of a meiotic atlas and chromosomal mapping of abundant genome elements in the orphan crop golden thistle (Scolymus hispanicus L.)","authors":"Ahmet L. Tek, Sevim D. Kara Öztürk, Hümeyra Yıldız Akkamış, Elif Sena Köksal, Emir Can Kaya","doi":"10.1016/j.plgene.2025.100493","DOIUrl":"10.1016/j.plgene.2025.100493","url":null,"abstract":"<div><div>Orphan crops have great potential for sustainable agricultural production. Meiosis in sexually reproducing organisms is a crucial process for maintaining the chromosome complement and ensuring genetic diversity by meiotic crossover and segregation of homologous chromosomes. Repetitive DNA elements differ in quantity, sequence, and distribution pattern among species. These elements are useful for taxonomic, phylogenetic, and evolutionary studies, as well as for establishing species-specific karyotypes. The golden thistle (<em>Scolymus hispanicus</em> L.), an orphan crop, is a diploid plant species (2<em>n</em> = 2<em>×</em> = 20) of the Asteraceae family and is common in the Mediterranean region. Despite some research on its phytochemical properties, there are few studies on its molecular biology, genetics, and cytogenetics. Therefore, we aimed to construct an atlas of the meiosis of golden thistle and to discover repetitive genome elements. A novel 180-bp repetitive DNA element, Sh180, of the golden thistle was identified by bioinformatic, molecular and cytogenetic methods. Chromosomal domains containing Sh180, the telomeric repeat TTTAGGGn, 5S and 35S rDNA were mapped on mitotic metaphase chromosomes by fluorescence in situ hybridization (FISH). The Sh180 signals were mapped in the subtelomeric regions of all mitotic metaphase chromosomes. Our findings provide the first information for future genetics and breeding studies such as meiotic, karyotype and phylogenetic analyses of this underutilized crop.</div></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"42 ","pages":"Article 100493"},"PeriodicalIF":2.2,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143388467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0