Plant Gene最新文献

{"title":"Gene editing for tolerance to temperature stress in plants: A review","authors":"Anindita Chakraborty ,&nbsp;Swapnila Choudhury ,&nbsp;Shikta Rani Kar ,&nbsp;Promita Deb ,&nbsp;Stephen J. Wylie","doi":"10.1016/j.plgene.2023.100439","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100439","url":null,"abstract":"<div><p>Temperature stress is a factor limiting agricultural production in many regions. High and low temperatures can cause irreversible damage to plants, affecting the development and profitability of crops, and are a threat to national and global food security. Raising agricultural outputs by developing elite crop cultivars capable of coping with damaging environmental temperatures is a primary objective of plant breeders. Gene editing systems offer opportunities to rapidly develop improved cultivars. Gene editing has already been applied to several species to improve valuable traits, including yield, quality, nutritional values and tolerance to abiotic and biotic stresses. This review focuses on the recent progress and future potential of gene editing in developing new cultivars with greater heat and cold stress tolerance.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"37 ","pages":"Article 100439"},"PeriodicalIF":0.0,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352407323000379/pdfft?md5=1c579e6efbc96c40353aacb1c708f8c0&pid=1-s2.0-S2352407323000379-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138413464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel associations of candidate marker genes with resistance to onion-fusarium basal rot interaction pathosystem","authors":"SaeidReza Poursakhi ,&nbsp;Hossein Ali Asadi-Gharneh ,&nbsp;Mehdi Nasr-Esfahani ,&nbsp;Zahra Abbasi ,&nbsp;Hamed Hassanzadeh Khankahdani","doi":"10.1016/j.plgene.2023.100440","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100440","url":null,"abstract":"<div><p>We evaluated the selected populations of eighteen open-pollinated short-day onion genotypes for FBR-Fusarium basal rot (<span><em>Fusarium oxysporum</em></span> f. sp. <em>cepae-FOC</em><span>) susceptibility; population genetic variation<span> via ISSR marker; and transcriptome analysis using qRT-PCR with six novel selected marker genes: </span></span><em>R1</em>, <em>R5</em>, <em>RGA29</em>, <span><em>lectin</em></span>, <em>LOX</em>, and <em>Osmotin</em>, at tree time post inoculation (wpi). Screening for resistance showed the average severity between 4.7 and 88.9%; of which, the lowest one was in ‘Saba’ and ‘Saba – HS (6.7 and 4.7%, respectively). ISSR analysis recorded 226 amplified bands, of which 160 bands showed polymorphism, of which ISSR1 and ISSR10 primers showed the best performance. We also found that following inoculation with <em>FOC</em> could regulate defense-related marker genes; <em>R1</em>, <em>PR5</em>, <em>Lectin</em>, <em>LOX</em>, <em>Osmotin</em>, and <em>RGA29</em> in resistant onion “Saba” and ‘Saba’-HS in comparison to susceptible and controls, non-inoculated ones ranging from 1.23 to 6.99 -fold significantly. Surprisingly, marker genes; <em>Lectin</em>, <em>LOX</em>, and <em>Osmotin</em> were also expressed to <em>FOC</em><span> simultaneous, though basically are resistance to other biotic and abiotic stress: </span><em>Lectin</em> to <span><em>Rhizoctonia solani</em></span>, Aphid, and major sap-sucking pests; <em>LOX</em><span> to root-knot and cyst nematode, </span><span><em>Heterodera glycines</em></span>; <em>Osmotin</em> to drought stredd, and oxidative burst in plants. This indicates the double, and or multiple roles of our selected marker genes covering two or more functions at a time. The findings introduce newly resistant onion genotypes, and also can be used in management programs to reduce damages caused by <em>FOC</em> disease. Cumulatively, the transcriptome-data provide novel-insights into the response of onions for improving onion-breeding to <em>FOC.</em></p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"37 ","pages":"Article 100440"},"PeriodicalIF":0.0,"publicationDate":"2023-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138390348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detecting QTLs controlling chlorophyll fluorescence parameters in Iranian wheat recombinant inbred lines","authors":"Narges Sahranavard ,&nbsp;Eisa Jorjani ,&nbsp;Hossein Sabouri ,&nbsp;Sharifeh Mohahamad Alegh ,&nbsp;Mahnaz Katouzi","doi":"10.1016/j.plgene.2023.100437","DOIUrl":"10.1016/j.plgene.2023.100437","url":null,"abstract":"<div><p><span>Measurement of chlorophyll fluorescence<span> is one of the methods to detect a disorder in the photosynthetic system<span>, which reflects the photochemical state of the plant. Identification of the genetic structure of chlorophyll fluorescence parameters can provide useful solutions for breeding varieties with higher potential. The present study was conducted with the aim of identifying quantitative loci (QTL) related to chlorophyll fluorescence parameters in an Iranian RILs<span> wheat population. One hundred and twenty F8 RILs of wheat derived from crossing Kohdasht (KHD) and Gonbad (GND) cultivars in the form of an alpha lattice design were studied in 2019 and 2020. The analysis of QTLs was performed in the software R using the package QTL.gCIMapping.GUI v2.0. The genetic map was constructed with 423 SSR markers, 21 CBDP markers, 58 ISJ markers, and 19 SCoT markers (521 polymorphic alleles) distributed on 21 wheat chromosomes. This map covered 3167.9 cM of the wheat genome and had an average marker spacing of 6.1, 6, and 6.2 cM for the A, B, and D genomes, respectively. Thirteen of the fourteen QTLs discovered in 2019 and four of the nine QTLs discovered in 2020 had an explanatory coefficient greater than 15% and were considered major QTLs. In 2019, pleiotropic QTLs were found at position 1.79 cM on chromosome 3A and between two markers Xwmc11-3A and BARC1177. In 2020, two QTLs, qABS/ RC </span></span></span></span><img>1B and qTRo/ RC <img>1B were located at position 36.80 cM 1B and between two markers Xgpw4331-1B and Xgpw5162-1B, and two QTLs, qETo/ RC-5D and qREo / RC-5D were located at position 63.25 cM chromosome 5D and correspond to the position of marker cfd266. Pleiotropic QTLs as well as major QTLs can be used in marker-assisted selection for chlorophyll fluorescence traits in wheat breeding projects.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"37 ","pages":"Article 100437"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135614734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population diversity analysis of an underutilized legume, winged bean (Psophocarpus tetragonolobus (L.) DC.) using ISSR markers","authors":"V. Kamal Kumar,&nbsp;R. Rajalakshmi","doi":"10.1016/j.plgene.2023.100436","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100436","url":null,"abstract":"<div><p>Seventy genotypes of winged bean representing five populations, include the, genotypes from India (IN), Thailand (TH), Nigeria (NI), Ghana (GH) and Papua New, Guinea (PNG) were taken for the study. Widely accepted population analysis tools;, POPGene Ver.1.32, GenAlEx Ver. 6.5, Splits Tree Ver.4.15.1, STRUCTURE Ver., 2.3.4 and Structure Harvester Ver.6.0 and MVSP Ver.3.22 were used for data, interpretation. Results revealed that the highest diversity was observed in Thailand, population. Genetic diversity between populations was not related to geography. All the, Indian samples except PT-1 grouped together, one Thailand sample (PT-33) was, totally different from other Thailand samples and PT-69 of PNG highly distinct from, other samples. Cluster analysis revealed that four Thailand samples (PT-31, PT-32, PT-48, and PT-49) two Indian samples (PT-28 and PT-29) and two Thailand samples, (PT-42 and PT-46) are genetically closer with less Euclidean distance of 0, whereas, PT-39 showed a maximum distance of 0.87. From this study, PT-33 (EC 178272), PT-, 69 (EC 38821 P2) and PT-39 (EC 178291) could be labelled as genetic variants.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"36 ","pages":"Article 100436"},"PeriodicalIF":0.0,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heterosis of quality protein maize inbred lines for agronomic traits and association with genetic distances based on SSR and phenotypic markers","authors":"Solomon Tayo Akinyosoye ,&nbsp;Morufat Oloruntoyin Balogun ,&nbsp;Samuel Adelowo Olakojo","doi":"10.1016/j.plgene.2023.100435","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100435","url":null,"abstract":"<div><p>Due to high cost, limited labour, and longer time required in hybrid development, identifying promising hybrids at an early stage without large-scale yield trials is crucial. Therefore this study estimated heterosis of quality protein maize hybrids for grain yield (GY) and other agronomic traits and investigated relationship of genetic and phenotypic distances (GD, PD) of inbreds with hybrid performance (HP) and mid-and-better parent heterosis (MPH, BPH). Forty-five hybrids generated by half-diallel with 10 inbreds were evaluated for GY and other agronomic traits at three locations. The yield trials were conducted for two years (2017 and 2018 cropping seasons). The GD and PD were 0.45 and 0.40 based on SSR and phenotypic data, respectively, this shows moderate genetic variation existed among inbreds. GY had highest MPH (77.55%) and BPH (53.96%) with hybrid TZEEQI-9 × TZEEQI-16 having highest MPH (382.8%) and BPH (331.7%), across locations. Positive and significant association existed between SSR-GD with HP, MPH and BPH for ear length, kernels/row and 100-seed weight (<em>r</em> = 0.31** to 0.41**), this suggests these traits can be predicted from SSR-based GD of parents. These traits could be used for indirect selection for yield improvement. However, negative correlation was obtained between SSR-GD with HP, MPH and BPH for anthesis and silking (<em>r</em> = −0.29 to −0.03), this indicates the dominance for earliness to flowering and silking than their parental lines. The two distance measures can be used to differentiate between maize inbreds and assigned them to different heterotic groups and Breeders can therefore prioritize crosses with high heterosis and desirable phenotypic traits, saving time, resources, and field space.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"36 ","pages":"Article 100435"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome wide identification and expression profiling of PYL genes in barley","authors":"Ali Shahzad ,&nbsp;Muhammad Shahzad ,&nbsp;Muhammad Imran ,&nbsp;Hameed Gul ,&nbsp;Shareef Gul","doi":"10.1016/j.plgene.2023.100434","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100434","url":null,"abstract":"<div><p><em>PYLs</em> (pyrabactin resistance1/PYR1-like) sense ABA, an essential phytohormone that regulates plant growth and stress responses. <em>PYLs</em> act as the main controllers of ABA stress signaling in plants. In this study, a total of 10 <em>HvPYLs</em> were discovered in the barley genome using an in silico genome search technique. These <em>HvPYLs</em> were then grouped into 3 subfamilies based on a phylogenetic analysis that compared them to the genomes of Arabidopsis, Brachypodium, rice, and maize. These HvPYLs demonstrated conserved motif compositions and identical protein structures across clades. Additionally, this study includes detailed investigations of gene structure variations, chromosomal distributions, cis-regulatory elements, protein-protein interactions, expression profiles in different tissues, and stress responses. We identified various cis-elements in the <em>HvPYL</em> promoter regions related to plant development and stresses indicating their potential roles in development and stress management. Our analysis of the interaction network has identified that <em>HvPYLs</em> can interact with key components of the ABA signaling pathway, demonstrating the critical regulatory functions of <em>HvPYL</em> genes in managing stress and growth in barley. These findings provide a basis for future research aimed at exploring the functions of <em>PYL</em> genes, with the ultimate goal of enhancing stress tolerance in barley and other related species.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"36 ","pages":"Article 100434"},"PeriodicalIF":0.0,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive transcriptional analysis unveils salt stress-regulated key pathways in Suaeda salsa leaves","authors":"Shima Jamalirad ,&nbsp;Mohammad Reza Azimi ,&nbsp;Nayer Azam Khoshkholgh Sima ,&nbsp;Mehrshad Zeinalabedini ,&nbsp;Laleh Karimi Farsad ,&nbsp;Ghasem Hosseini Salekdeh ,&nbsp;Mohammad Reza Ghaffari","doi":"10.1016/j.plgene.2023.100433","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100433","url":null,"abstract":"<div><p><em>Suaeda salsa</em> represents a promising halophyte model for investigating the mechanisms underlying salt tolerance in plants. However, the molecular mechanisms regulating seedling establishment of <em>Suaeda salsa</em> remain unknown. Thus, the current study was conducted to understand the underlying regulatory mechanisms in <em>Suaeda salsa</em> leaves exposed to 0 mM, 200 mM, 400 mM, and 800 mM NaCl using high-throughput RNA sequencing. The number of differentially expressed transcripts substantially increased when the salinity level elevated, suggesting major transcriptional reorganization in response to salinity stress. Importantly, the differentially identified transcripts were mostly salt responsive genes belonging to specific categories like plant hormone signaling, solute transport and nutrient uptake, protein metabolism, and transcriptional regulation. Specifically, higher level of salt changed 339 genes markedly implicated in transcriptional regulation such as MYB, MADS-box, NAC, ERF, WRKY, HB families, as well as genes involved in protein metabolism such as the tyrosine-like protein and ATG members and autophagic cargo receptor protein. This indicated important key players to high salt tolerance. Collectively, our findings revealed the crucial regulatory pathways underlying the salt tolerance of <em>Suaeda salsa</em> through a cascade that includes signal perception and transduction, and transcription factors that regulate the downstream response genes such as those involved in protein metabolism, solute and nutrient transport for salt stress adaptation.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"36 ","pages":"Article 100433"},"PeriodicalIF":0.0,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the active site of a germin like protein 1 as an oxidative stress defense enzyme in plants","authors":"Dure Shahwar ,&nbsp;Farah Deeba ,&nbsp;Irtiza Hussain ,&nbsp;S.M. Saqlan Naqvi ,&nbsp;Fatema S. Alatawi ,&nbsp;Awatif M.E. Omran ,&nbsp;Anam Moosa ,&nbsp;Faisal Zulfiqar","doi":"10.1016/j.plgene.2023.100432","DOIUrl":"https://doi.org/10.1016/j.plgene.2023.100432","url":null,"abstract":"<div><p>Glycosylated proteins like germin-like proteins (GLPs) are incredibly diverse inside the kingdom Plantae, and mostly GLPs exhibit superoxide dismutase (SOD) function. Identification of catalytic residues is important for understanding the mechanism of enzyme-catalyzed reactions. The increased bioactivity of SOD was observed when <em>OsRGLP1</em> was over-expressed in tobacco. The purpose of the current work was to identify and characterize the active site of <em>OsRGLP1</em>. Bioinformatics tools were used to predict the three-dimensional structure of <em>OsRGLP1</em> and the shape of residues implicated in the substrate and metal ion binding. The role of predicted active site residues (E116, H109, H111, and H157) in the structure-function relationship in <em>OsRGLP1</em> was investigated by site-directed mutagenesis where each residue was substituted with glycine. These amino acids are highly conserved among GLP family and structural data have implicated these residues in substrate binding at the active site. Transient transformation of tobacco plants was performed to further study these loss-of-function mutants. To investigate the impact of the mutation on SOD activity, these transgenic plants were employed as a source of mutant and native proteins for SOD activity assays. The SOD assay results revealed a complete loss of activity in all mutants, supporting the crucial role of these residues for metal ion binding in the enzyme active site.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"36 ","pages":"Article 100432"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50182767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B × R cross de rived parental line development using breeding value: A new model for hybrid rice parental line development","authors":"Md. Ruhul Quddus ,&nbsp;Md. Jamil Hasan ,&nbsp;Mst. Umma Kulsum ,&nbsp;Satyen Mondal","doi":"10.1016/j.plgene.2023.100431","DOIUrl":"10.1016/j.plgene.2023.100431","url":null,"abstract":"<div><p>Three-line hybrid rice system is the most successful and widely practiced method around the world. Hybrid rice breeders have used B × B, A × R and R × R (R = Restorer line, B = Maintainer line, A = CMS line) scheme of parental line improvement frequently and avoided B × R and R × B scheme. As a result, female parents lack the genetic diversity carried by R lines. But B × R and R × B mating have great potential to produce high value parental lines of hybrid rice and overcome the limitation of the previous approach. We have demonstrated a new method for three-line hybrid system to minimize the barrier of crossing in parent selection for developing new elite maintainers and restorers. Parental combinations were selected based on breeding value of the genotypes. Breeding values were estimated based on ancestor, pedigree information and yield data of 74 test genotype to select parents for restorer and maintainer line improvement. This new protocol allows (B × R), (R × B) (R × Elite) and (B × Elite) improvement technique to bring out high yielding diverse B and R lines. This B line will be used for developing new A line in the genetic background of B line. Doubled haploid and RGA i.e. rapid generation advance tools of breeding will save the precious time and reduce breeding cycle length; and large population size will increase selection accuracy. We have predicted the genetic gain in parental line development for four parental cross using the studied 74 genotypes for doubled haploid and rapid generation advance methods. Our objectives were to demonstrate the new breeding approach plus breeding value and positive dominant gene effect-based parent selection strategy. We are hopeful about the new method that hybrid rice breeders across the world will extract benefit utilizing the new methodology of hybrid rice parental line development.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"36 ","pages":"Article 100431"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49153640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing garden pea germplasm for powdery mildew resistance through disease phenotyping and genotyping using molecular markers","authors":"Priyambada Sharada,&nbsp;Ragiba Makandar","doi":"10.1016/j.plgene.2023.100425","DOIUrl":"10.1016/j.plgene.2023.100425","url":null,"abstract":"<div><p><em>Erysiphe pisi</em> is one among the other causal agents of powdery mildew infection in garden pea. So far, the resistance genes comprising two recessive (<em>er1</em> and <em>er</em>2) and one dominant (<em>Er3</em>) have been reported to confer resistance to powdery mildew in garden pea. A set of 46 pea genotypes were screened against the <em>E. pisi</em> isolate- Ep01 in greenhouse conditions to identify resistant genotypes. Disease reaction and genotyping were carried out to test for resistance/ susceptibility through gene-specific sequence characterized amplified region (SCAR) markers. The presence of the resistance alleles in the control pea genotypes- JI2302 for <em>er1</em> gene, JI2480 for <em>er2</em> gene and P660–4 for <em>Er3</em> was confirmed through their respective gene-specific markers. The pea genotype- Arkel served as a negative control. Screening of the pea germplasm revealed 3 genotypes as highly resistant, 6 genotypes as resistant while 10 genotypes were moderately resistant, 13 genotypes were moderately susceptible, 9 genotypes were susceptible and 5 were highly susceptible. The molecular marker for <em>er1</em> resistance gene Sc-OPE-16₁₆₀₀ was found to be segregating in most of the pea genotypes except in the susceptible control- Arkel. The marker- ScX17_1400 for <em>er2</em> gene was found to be in homozygous condition in the resistant pea genotypes while the marker associated with <em>Er3</em> resistance, SCW4₆₃₇, was found to be in heterozygous condition in majority of the pea genotypes except in the resistant control genotype- P660–4.</p></div>","PeriodicalId":38041,"journal":{"name":"Plant Gene","volume":"35 ","pages":"Article 100425"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48180112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}