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Current protocols in mouse biology最新文献

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Computational Analysis of Neonatal Mouse Ultrasonic Vocalization 新生小鼠超声发声的计算分析
Current protocols in mouse biology Pub Date : 2018-05-21 DOI: 10.1002/cpmo.46
Pilib Ó Broin, Michael V. Beckert, Tomohisa Takahashi, Takeshi Izumi, Kenny Ye, Gina Kang, Patricia Pouso, Mackenzie Topolski, Jose L. Pena, Noboru Hiroi
{"title":"Computational Analysis of Neonatal Mouse Ultrasonic Vocalization","authors":"Pilib Ó Broin,&nbsp;Michael V. Beckert,&nbsp;Tomohisa Takahashi,&nbsp;Takeshi Izumi,&nbsp;Kenny Ye,&nbsp;Gina Kang,&nbsp;Patricia Pouso,&nbsp;Mackenzie Topolski,&nbsp;Jose L. Pena,&nbsp;Noboru Hiroi","doi":"10.1002/cpmo.46","DOIUrl":"10.1002/cpmo.46","url":null,"abstract":"<p>Neonatal vocalization is structurally altered in mouse models of autism spectrum disorder (ASD). Our published data showed that pup vocalization, under conditions of maternal separation, contains sequences whose alterations in a genetic mouse model of ASD impair social communication between pups and mothers. We describe details of a method which reveals the statistical structure of call sequences that are functionally critical for optimal maternal care. Entropy analysis determines the degree of non-random call sequencing. A Markov model determines the actual call sequences used by pups. Sparse partial least squares discriminant analysis (sPLS-DA) identifies call sequences that differentiate groups and reveals the degrees of individual variability in call sequences between groups. These three sets of analyses can be used to identify the otherwise hidden call structure that is altered in mouse models of developmental neuropsychiatric disorders, including not only autism but also schizophrenia. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.46","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10026813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Generation and Identification of Mutations Resulting in Chronic and Age-Related Phenotypes in Mice 小鼠慢性和年龄相关表型突变的产生和鉴定
Current protocols in mouse biology Pub Date : 2018-05-21 DOI: 10.1002/cpmo.42
Andrew Blease, Thomas Nicol, Sara Falcone, Becky Starbuck, Simon Greenaway, Marie Hutchinson, Paul K. Potter
{"title":"Generation and Identification of Mutations Resulting in Chronic and Age-Related Phenotypes in Mice","authors":"Andrew Blease,&nbsp;Thomas Nicol,&nbsp;Sara Falcone,&nbsp;Becky Starbuck,&nbsp;Simon Greenaway,&nbsp;Marie Hutchinson,&nbsp;Paul K. Potter","doi":"10.1002/cpmo.42","DOIUrl":"10.1002/cpmo.42","url":null,"abstract":"<p>Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging is a significant risk factor for a range of significant diseases. To improve modeling of such diseases, we should incorporate aging into our models. Many systems are affected by aging, with a decline in mitochondrial function, an increase in senescence, a loss of resilience, telomere shortening, and a decline in immune function being key factors in the increased susceptibility to disease that is associated with aging. To develop novel models of age-related disease, we undertook a phenotype-driven screen of a pipeline of mutagenized mice. Here, we describe some of the underlying protocols and outline important aspects to consider when studying aged mice. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.42","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36244437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Measuring Preweaning Sensorial and Motor Development in the Mouse. 小鼠断奶前感觉和运动发育的测量。
Current protocols in mouse biology Pub Date : 2018-03-01 DOI: 10.1002/cpmo.41
Pierre L Roubertoux, Adeline Ghata, Michèle Carlier
{"title":"Measuring Preweaning Sensorial and Motor Development in the Mouse.","authors":"Pierre L Roubertoux,&nbsp;Adeline Ghata,&nbsp;Michèle Carlier","doi":"10.1002/cpmo.41","DOIUrl":"https://doi.org/10.1002/cpmo.41","url":null,"abstract":"<p><p>The immaturity at birth and the slowness of ontogenic processes in mice provide the opportunity to measure rates of development. We describe here 18 measures covering the sensorial and motor onset from birth to weaning. The measures are non-invasive, making a follow-up strategy possible. The first basic protocol indicates how to produce mice with known conceptional or chronological age, as the control of the age is a prerequisite to compare rates of development in groups of mice. The second basic protocol describes a set of methods for identifying the pups during a follow-up study. A third basic protocol describes testing newborn mice for the appearance of sensorial and motor abilities in a follow-up design. Taken together, the three protocols make possible the validation of potential murine models of interest for understanding human developmental disorders. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 1","pages":"54-78"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.41","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36337443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease. 利用 Cas9 RNA 引导的核酸酶在小鼠体内制造点突变和表位标记的等位基因
Current protocols in mouse biology Pub Date : 2018-03-01 DOI: 10.1002/cpmo.40
Marina Gertsenstein, Lauryl M J Nutter
{"title":"Engineering Point Mutant and Epitope-Tagged Alleles in Mice Using Cas9 RNA-Guided Nuclease.","authors":"Marina Gertsenstein, Lauryl M J Nutter","doi":"10.1002/cpmo.40","DOIUrl":"10.1002/cpmo.40","url":null,"abstract":"<p><p>Mice carrying patient-associated point mutations are powerful tools to define the causality of single-nucleotide variants to disease states. Epitope tags enable immuno-based studies of genes for which no antibodies are available. These alleles enable detailed and precise developmental, mechanistic, and translational research. The first step in generating these alleles is to identify within the target sequence-the orthologous sequence for point mutations or the N or C terminus for epitope tags-appropriate Cas9 protospacer sequences. Subsequent steps include design and acquisition of a single-stranded oligonucleotide repair template, synthesis of a single guide RNA (sgRNA), collection of zygotes, and microinjection or electroporation of zygotes with Cas9 mRNA or protein, sgRNA, and repair template followed by screening of born mice for the presence of the desired sequence change. Quality control of mouse lines includes screening for random or multicopy insertions of the repair template and, depending on sgRNA sequence, off-target mutations introduced by Cas9. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 1","pages":"28-53"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9249120/pdf/nihms926466.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36339622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Noninvasive Electroretinographic Procedures for the Study of the Mouse Retina. 研究小鼠视网膜的无创视网膜电成像方法。
Current protocols in mouse biology Pub Date : 2018-03-01 DOI: 10.1002/cpmo.39
Junzo Kinoshita, Neal S Peachey
{"title":"Noninvasive Electroretinographic Procedures for the Study of the Mouse Retina.","authors":"Junzo Kinoshita,&nbsp;Neal S Peachey","doi":"10.1002/cpmo.39","DOIUrl":"https://doi.org/10.1002/cpmo.39","url":null,"abstract":"<p><p>Overall retinal function can be monitored by recording the light-evoked response of the eye at the corneal surface. The major components of the electroretinogram (ERG) provide important information regarding the functional status of many retinal cell types including rod photoreceptors, cone photoreceptors, bipolar cells, and the retinal pigment epithelium (RPE). The ERG can be readily recorded from mice, and this unit describes procedures for mouse anesthesia and the use of stimulation and recording procedures for measuring ERGs that reflect the response properties of different retinal cell types. Through these, the mouse ERG provides a noninvasive approach to measure multiple aspects of outer retinal function, including the status of the initial rod and cone pathways, rod photoreceptor deactivation, rod dark adaptation, the photoreceptor-to-bipolar cell synapse, and the RPE. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 1","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.39","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36338593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
Production of Humanized Mice through Stem Cell Transfer. 通过干细胞移植生产人源化小鼠。
Current protocols in mouse biology Pub Date : 2018-03-01 Epub Date: 2018-03-30 DOI: 10.1002/cpmo.38
Devra D Huey, Stefan Niewiesk
{"title":"Production of Humanized Mice through Stem Cell Transfer.","authors":"Devra D Huey,&nbsp;Stefan Niewiesk","doi":"10.1002/cpmo.38","DOIUrl":"https://doi.org/10.1002/cpmo.38","url":null,"abstract":"<p><p>The development of humanized mice has become a prominent tool for translational animal studies of human diseases. Here we show how immune deficient mice can be \"humanized\" by injections of human umbilical cord stem cells. The engraftment of these cells and development into human lymphocytes has been possible because of the development of novel severely immune deficient mouse strains. Here we present proven protocols for the generation and analysis of humanized mice on the NSG mouse background.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 1","pages":"17-27"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.38","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36297652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Analysis of Neuropsychiatric Disease-Related Functional Neuroanatomical Markers in Mice. 小鼠神经精神疾病相关功能神经解剖学标志物分析。
Current protocols in mouse biology Pub Date : 2018-03-01 DOI: 10.1002/cpmo.37
Lillian Garrett, Marie-Claire Ung, Tamara Heermann, Kristina M Niedermeier, Sabine Hölter
{"title":"Analysis of Neuropsychiatric Disease-Related Functional Neuroanatomical Markers in Mice.","authors":"Lillian Garrett,&nbsp;Marie-Claire Ung,&nbsp;Tamara Heermann,&nbsp;Kristina M Niedermeier,&nbsp;Sabine Hölter","doi":"10.1002/cpmo.37","DOIUrl":"https://doi.org/10.1002/cpmo.37","url":null,"abstract":"<p><p>A better alignment of preclinical and clinical neurobiological measures could help improve neuropsychiatric disease therapeutic development. This unit describes a compendium of hypothesis-driven neuroanatomical phenotyping strategies to be employed in genetic mouse models. Using neuropsychiatric disease-based alterations as a guide, these are histological and immunohistochemical methodologies also applied to human tissue. They include quantification assays of neurochemical-, newly born neuron- and glial-cell markers, synaptic proteins, regional volumetrics, dendritic complexity and spine number as well as an index of excitation/inhibition balance. The techniques can be implemented in isolation or to complement concordant behavioral and electrophysiological analyses. Each outcome will provide functional detail necessary to decipher underlying neural circuit abnormalities associated with a brain-related phenotype in mice. Experimental design, timing, anticipated results and potential pitfalls are discussed. © 2018 by John Wiley & Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"8 1","pages":"79-128"},"PeriodicalIF":0.0,"publicationDate":"2018-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.37","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36336634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
A Mouse Model of West Nile Virus Infection 西尼罗病毒感染小鼠模型
Current protocols in mouse biology Pub Date : 2018-02-13 DOI: 10.1002/cpmo.33
Jessica B. Graham, Jessica L. Swarts, Jennifer M. Lund
{"title":"A Mouse Model of West Nile Virus Infection","authors":"Jessica B. Graham,&nbsp;Jessica L. Swarts,&nbsp;Jennifer M. Lund","doi":"10.1002/cpmo.33","DOIUrl":"10.1002/cpmo.33","url":null,"abstract":"<p>The use of a mouse model to study the breadth of symptoms and disease severity seen in human West Nile virus (WNV) infection can provide insight into the kinetics of the immune response and the specific pathways responsible for control of WNV infection and viral clearance. Here, we provide protocols for performing WNV infection of mice, as well as complete immunophenotyping analysis of the cellular immune response to infection in both the periphery and the central nervous system in a mouse model of WNV infection. © 2017 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.33","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35674754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Assessing Sociability, Social Memory, and Pup Retrieval in Mice 评估小鼠的社交能力、社会记忆和幼犬检索
Current protocols in mouse biology Pub Date : 2018-02-13 DOI: 10.1002/cpmo.36
Annemarie Zimprich, Jörn Niessing, Lior Cohen, Lillian Garrett, Jan Einicke, Bettina Sperling, Mathias V. Schmidt, Sabine M. Hölter
{"title":"Assessing Sociability, Social Memory, and Pup Retrieval in Mice","authors":"Annemarie Zimprich,&nbsp;Jörn Niessing,&nbsp;Lior Cohen,&nbsp;Lillian Garrett,&nbsp;Jan Einicke,&nbsp;Bettina Sperling,&nbsp;Mathias V. Schmidt,&nbsp;Sabine M. Hölter","doi":"10.1002/cpmo.36","DOIUrl":"10.1002/cpmo.36","url":null,"abstract":"<p>Adaptive social behavior is important in mammals, both for the well-being of the individual and for the thriving of the species. Dysfunctions in social behavior occur in many neurodevelopmental and psychiatric diseases, and research into the genetic components of disease-relevant social deficits can open up new avenues for understanding the underlying biological mechanisms and therapeutic interventions. Genetically modified mouse models are particularly useful in this respect, and robust experimental protocols are needed to reliably assess relevant social behavior phenotypes. Here we describe in detail three protocols to quantitatively measure sociability, one of the most frequently investigated social behavior phenotypes in mice, using a three-chamber sociability test. These protocols can be extended to also assess social memory. In addition, we provide a detailed protocol on pup retrieval, which is a particularly robust maternal behavior amenable to various scientific questions. © 2017 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.36","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35674752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Evaluation of the Estrous Cycle, Reproductive Tract, and Mammary Gland in Female Mice 雌性小鼠的发情周期、生殖道和乳腺的评估
Current protocols in mouse biology Pub Date : 2018-02-13 DOI: 10.1002/cpmo.35
Justin D. Vidal, Adam J. Filgo
{"title":"Evaluation of the Estrous Cycle, Reproductive Tract, and Mammary Gland in Female Mice","authors":"Justin D. Vidal,&nbsp;Adam J. Filgo","doi":"10.1002/cpmo.35","DOIUrl":"10.1002/cpmo.35","url":null,"abstract":"<p>Evaluation of the female reproductive system is an important part of basic reproductive biology research, toxicology testing, and mutant mouse phenotype assessment. The female reproductive system is dynamic and the onset of puberty and the normal changes observed during estrous cyclicity can create challenges for an investigator. Experimental work in the female mouse requires an understanding of the potential impact of the estrous cycle and tracking normal changes throughout the cycle allows for control of this key variable. The estrous cycle can be evaluated using vaginal cytology, which provides the researcher a daily assessment of the entire reproductive endocrine axis and allows for samples to be collected at precise times within the cycle. These protocols describe the basic approach to evaluating the onset of cyclicity, tracking the normal cycle, and collection and microscopic evaluation of the reproductive system and mammary gland in the mouse. © 2017 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.35","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35674755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
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