Computational Analysis of Neonatal Mouse Ultrasonic Vocalization

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2018-05-21 DOI:10.1002/cpmo.46

Pilib Ó Broin, Michael V. Beckert, Tomohisa Takahashi, Takeshi Izumi, Kenny Ye, Gina Kang, Patricia Pouso, Mackenzie Topolski, Jose L. Pena, Noboru Hiroi

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引用次数: 8

Abstract

Neonatal vocalization is structurally altered in mouse models of autism spectrum disorder (ASD). Our published data showed that pup vocalization, under conditions of maternal separation, contains sequences whose alterations in a genetic mouse model of ASD impair social communication between pups and mothers. We describe details of a method which reveals the statistical structure of call sequences that are functionally critical for optimal maternal care. Entropy analysis determines the degree of non-random call sequencing. A Markov model determines the actual call sequences used by pups. Sparse partial least squares discriminant analysis (sPLS-DA) identifies call sequences that differentiate groups and reveals the degrees of individual variability in call sequences between groups. These three sets of analyses can be used to identify the otherwise hidden call structure that is altered in mouse models of developmental neuropsychiatric disorders, including not only autism but also schizophrenia. © 2018 by John Wiley & Sons, Inc.

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新生小鼠超声发声的计算分析

在自闭症谱系障碍(ASD)小鼠模型中，新生儿发声在结构上发生了改变。我们发表的数据表明，在母亲分离的条件下，幼鼠的发声包含一些序列，这些序列在ASD遗传小鼠模型中发生了改变，损害了幼鼠和母亲之间的社会交流。我们描述了一种方法的细节，该方法揭示了呼叫序列的统计结构，这对最佳的产妇护理功能至关重要。熵分析决定了非随机调用排序的程度。马尔可夫模型决定了幼崽使用的实际呼叫序列。稀疏偏最小二乘判别分析(sPLS-DA)可以识别区分群体的呼叫序列，并揭示群体之间呼叫序列的个体变异程度。这三组分析可以用来识别在发育性神经精神疾病(不仅包括自闭症，还包括精神分裂症)的小鼠模型中被改变的隐藏的呼叫结构。©2018 by John Wiley &儿子,Inc。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.