Generation and Identification of Mutations Resulting in Chronic and Age-Related Phenotypes in Mice

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2018-05-21 DOI:10.1002/cpmo.42

Andrew Blease, Thomas Nicol, Sara Falcone, Becky Starbuck, Simon Greenaway, Marie Hutchinson, Paul K. Potter

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引用次数: 2

Abstract

Aging is inevitable, and our society must deal with the consequences: namely, an increased incidence of disease and ill health. Many mouse models of disease are acute or early onset or are induced in young mice, despite the fact that aging is a significant risk factor for a range of significant diseases. To improve modeling of such diseases, we should incorporate aging into our models. Many systems are affected by aging, with a decline in mitochondrial function, an increase in senescence, a loss of resilience, telomere shortening, and a decline in immune function being key factors in the increased susceptibility to disease that is associated with aging. To develop novel models of age-related disease, we undertook a phenotype-driven screen of a pipeline of mutagenized mice. Here, we describe some of the underlying protocols and outline important aspects to consider when studying aged mice. © 2018 by John Wiley & Sons, Inc.

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小鼠慢性和年龄相关表型突变的产生和鉴定

老龄化是不可避免的，我们的社会必须应对其后果:即疾病和健康状况不佳的发生率增加。尽管衰老是一系列重大疾病的重要风险因素，但许多疾病的小鼠模型是急性的或早发的，或者是在年轻小鼠中诱导的。为了改进这类疾病的建模，我们应该将衰老纳入我们的模型。许多系统受到衰老的影响，线粒体功能下降、衰老加剧、恢复力丧失、端粒缩短和免疫功能下降是与衰老相关的疾病易感性增加的关键因素。为了开发与年龄相关疾病的新模型，我们对一系列突变小鼠进行了表型驱动筛选。在这里，我们描述了一些潜在的协议，并概述了研究老年小鼠时需要考虑的重要方面。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.