小鼠断奶前感觉和运动发育的测量。

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2018-03-01 DOI:10.1002/cpmo.41

Pierre L Roubertoux, Adeline Ghata, Michèle Carlier

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引用次数: 11

摘要

小鼠出生时的不成熟和个体形成过程的缓慢为测量发育速度提供了机会。我们在这里描述了18项措施，涵盖了从出生到断奶的感觉和运动发病。这些措施是非侵入性的，使后续策略成为可能。第一个基本方案表明如何产生已知的概念年龄或实足年龄的小鼠，因为年龄的控制是比较各组小鼠发育速度的先决条件。第二个基本协议描述了一套在后续研究中识别幼崽的方法。第三个基本方案描述了在后续设计中测试新生小鼠的感觉和运动能力的外观。综上所述，这三种方案使验证潜在的小鼠模型成为可能，这些模型有助于理解人类发育障碍。©2018 by John Wiley & Sons, Inc。

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Measuring Preweaning Sensorial and Motor Development in the Mouse.

The immaturity at birth and the slowness of ontogenic processes in mice provide the opportunity to measure rates of development. We describe here 18 measures covering the sensorial and motor onset from birth to weaning. The measures are non-invasive, making a follow-up strategy possible. The first basic protocol indicates how to produce mice with known conceptional or chronological age, as the control of the age is a prerequisite to compare rates of development in groups of mice. The second basic protocol describes a set of methods for identifying the pups during a follow-up study. A third basic protocol describes testing newborn mice for the appearance of sensorial and motor abilities in a follow-up design. Taken together, the three protocols make possible the validation of potential murine models of interest for understanding human developmental disorders. © 2018 by John Wiley & Sons, Inc.

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.