Noninvasive Electroretinographic Procedures for the Study of the Mouse Retina.

Q1 Agricultural and Biological Sciences
Junzo Kinoshita, Neal S Peachey
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引用次数: 24

Abstract

Overall retinal function can be monitored by recording the light-evoked response of the eye at the corneal surface. The major components of the electroretinogram (ERG) provide important information regarding the functional status of many retinal cell types including rod photoreceptors, cone photoreceptors, bipolar cells, and the retinal pigment epithelium (RPE). The ERG can be readily recorded from mice, and this unit describes procedures for mouse anesthesia and the use of stimulation and recording procedures for measuring ERGs that reflect the response properties of different retinal cell types. Through these, the mouse ERG provides a noninvasive approach to measure multiple aspects of outer retinal function, including the status of the initial rod and cone pathways, rod photoreceptor deactivation, rod dark adaptation, the photoreceptor-to-bipolar cell synapse, and the RPE. © 2018 by John Wiley & Sons, Inc.

Abstract Image

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研究小鼠视网膜的无创视网膜电成像方法。
通过记录角膜表面的光诱发反应,可以监测视网膜的整体功能。视网膜电图(ERG)的主要组成部分提供了许多视网膜细胞类型的功能状态的重要信息,包括视杆光感受器、视锥光感受器、双极细胞和视网膜色素上皮(RPE)。小鼠的ERG可以很容易地记录下来,本单元描述了小鼠麻醉的程序,以及使用刺激和记录程序来测量反映不同视网膜细胞类型反应特性的ERG。通过这些,小鼠ERG提供了一种非侵入性的方法来测量视网膜外功能的多个方面,包括初始杆状和锥状通路的状态、杆状光感受器失活、杆状暗适应、光感受器-双极细胞突触和RPE。©2018 by John Wiley & Sons, Inc。
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来源期刊
Current protocols in mouse biology
Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology
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期刊介绍: Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.
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