Assessing Sociability, Social Memory, and Pup Retrieval in Mice

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2018-02-13 DOI:10.1002/cpmo.36

Annemarie Zimprich, Jörn Niessing, Lior Cohen, Lillian Garrett, Jan Einicke, Bettina Sperling, Mathias V. Schmidt, Sabine M. Hölter

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引用次数: 7

Abstract

Adaptive social behavior is important in mammals, both for the well-being of the individual and for the thriving of the species. Dysfunctions in social behavior occur in many neurodevelopmental and psychiatric diseases, and research into the genetic components of disease-relevant social deficits can open up new avenues for understanding the underlying biological mechanisms and therapeutic interventions. Genetically modified mouse models are particularly useful in this respect, and robust experimental protocols are needed to reliably assess relevant social behavior phenotypes. Here we describe in detail three protocols to quantitatively measure sociability, one of the most frequently investigated social behavior phenotypes in mice, using a three-chamber sociability test. These protocols can be extended to also assess social memory. In addition, we provide a detailed protocol on pup retrieval, which is a particularly robust maternal behavior amenable to various scientific questions. © 2017 by John Wiley & Sons, Inc.

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评估小鼠的社交能力、社会记忆和幼犬检索

适应社会行为对哺乳动物来说很重要，无论是对个体的幸福还是对物种的繁荣。社会行为功能障碍发生在许多神经发育和精神疾病中，对疾病相关社会缺陷的遗传成分的研究可以为理解潜在的生物学机制和治疗干预开辟新的途径。转基因小鼠模型在这方面特别有用，需要强有力的实验方案来可靠地评估相关的社会行为表型。在这里，我们详细描述了定量测量社交性的三种方案，社交性是小鼠中最常见的社会行为表型之一，使用三室社交性测试。这些协议也可以扩展到评估社会记忆。此外，我们提供了一个详细的协议幼犬检索，这是一个特别强大的母性行为适应各种科学问题。©2017 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.