Current protocols in mouse biology最新文献_第6页

The Study of Host Immune Responses Elicited by the Model Murine Hookworms Nippostrongylus brasiliensis and Heligmosomoides polygyrus 模型鼠钩虫巴西尼波圆线虫和多回Heligmosomoides诱导宿主免疫应答的研究

Current protocols in mouse biology Pub Date : 2018-02-13 DOI: 10.1002/cpmo.34

T. Bouchery, B. Volpe, K. Shah, L. Lebon, K. Filbey, G. LeGros, N. Harris

{"title":"The Study of Host Immune Responses Elicited by the Model Murine Hookworms Nippostrongylus brasiliensis and Heligmosomoides polygyrus","authors":"T. Bouchery, B. Volpe, K. Shah, L. Lebon, K. Filbey, G. LeGros, N. Harris","doi":"10.1002/cpmo.34","DOIUrl":"10.1002/cpmo.34","url":null,"abstract":"Hookworm infections (Necator americanus or Ancylostoma duodenale) represent a major neglected tropical disease, affecting approximately 700 million people worldwide, and can cause severe morbidity due to the need for these worms to feed on host blood. N. brasiliensis and H. polygrus, both rodent parasites, are the two most commonly employed laboratory models of experimental hookworm infection. Both parasites evoke type 2 immune responses, and their use has been instrumental in generating fundamental insight into the molecular mechanisms of type-2 immunity and for understanding how the immune response can control parasite numbers. Here we provide a complete set of methods by which to investigate the natural progression of infection and the host immunological responses in the lung and intestine of H. polygyrus– and N. brasiliensis–infected mice. Detailed information is included about the most important parasitological and immunological measurements to perform at each time point. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.34","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35674753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Inducing and Characterizing Liver Regeneration in Mice: Reliable Models, Essential “Readouts” and Critical Perspectives 诱导和表征小鼠肝脏再生:可靠的模型，必要的“读数”和关键的观点

Current protocols in mouse biology Pub Date : 2017-10-01 DOI: 10.1002/9780470942390.mo130087

Dimitrios C. Mastellos, Robert A. DeAngelis, John D. Lambris

{"title":"Inducing and Characterizing Liver Regeneration in Mice: Reliable Models, Essential “Readouts” and Critical Perspectives","authors":"Dimitrios C. Mastellos, Robert A. DeAngelis, John D. Lambris","doi":"10.1002/9780470942390.mo130087","DOIUrl":"10.1002/9780470942390.mo130087","url":null,"abstract":"Elucidating the molecular circuitry that regulates regenerative responses in mammals has recently attracted considerable attention because of its emerging impact on modern bioengineering, tissue replacement technologies, and organ transplantation. The liver is one of the few organs of the adult body that exhibits a prominent regenerative capacity in response to toxic injury, viral infection, or surgical resection. Over the years, mechanistic insights into the liver's regenerative potential have been provided by rodent models of chemical liver injury or surgical resection that faithfully recapitulate hallmarks of human pathophysiology and trigger robust hepatocyte proliferation leading to organ restoration. The advent of mouse transgenics has undeniably catalyzed the wider application of such models for researching liver pathobiology. This article provides a comprehensive overview of the most reliable and widely applied murine models of liver regeneration and also discusses helpful hints, considerations, and limitations related to the use of these models in liver regeneration studies. Curr. Protoc. Mouse Biol. 3:141-170 © 2013 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"3 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/9780470942390.mo130087","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32021617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Profiling of Single-Cell Transcriptomes 单细胞转录组分析

Current protocols in mouse biology Pub Date : 2017-09-08 DOI: 10.1002/cpmo.30

Wanze Chen, Vincent Gardeux, Antonio Meireles-Filho, Bart Deplancke

{"title":"Profiling of Single-Cell Transcriptomes","authors":"Wanze Chen, Vincent Gardeux, Antonio Meireles-Filho, Bart Deplancke","doi":"10.1002/cpmo.30","DOIUrl":"10.1002/cpmo.30","url":null,"abstract":"Complex biological systems are composed of multiple cell types whose transcriptional activity can vary due to differences in cell state, environmental stimulation, or intrinsic programs. Conventional bulk analysis methods capture the average transcriptional programs of the cell population, thus missing the unique cellular signature of each single cell. In recent years, the development of single-cell RNA-sequencing (scRNA-seq) technologies has provided a powerful approach to dissect the cellular heterogeneity of complex biological systems. However, such approaches require specialized equipment or are costly. In this article, we describe an improved Smart-seq2-based method to profile the transcriptome of hundreds of single cells simultaneously, without utilizing commercial kits or requiring any specialized single-cell capture/library preparation tools. Moreover, we introduce the Automated Single-cell Analysis Pipeline (ASAP), which allows researchers without strong computational expertise to explore scRNA-seq data using a wide range of commonly used algorithms and sophisticated visualization tools. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.30","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35484854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Pathology Evaluation of Developmental Phenotypes in Neonatal and Juvenile Mice 新生和幼年小鼠发育表型的病理评价

Current protocols in mouse biology Pub Date : 2017-09-08 DOI: 10.1002/cpmo.31

Brad Bolon, Susan Newbigging, Kelli L. Boyd

引用次数: 3

Using Vascular Landmarks to Orient 3D Optical Coherence Tomography Images of the Mouse Eye 利用血管地标定位小鼠眼的三维光学相干断层成像

Current protocols in mouse biology Pub Date : 2017-09-08 DOI: 10.1002/cpmo.32

Mark P. Krebs

引用次数: 3

Mouse Model of Burn Wound and Infection: Thermal (Hot Air) Lesion-Induced Immunosuppression 烧伤创面和感染小鼠模型:热(热空气)损伤诱导的免疫抑制

Current protocols in mouse biology Pub Date : 2017-06-19 DOI: 10.1002/cpmo.25

Henrik Calum, Niels Høiby, Claus Moser

引用次数: 15

Application of SWATH Proteomics to Mouse Biology SWATH蛋白质组学在小鼠生物学中的应用

Current protocols in mouse biology Pub Date : 2017-06-19 DOI: 10.1002/cpmo.28

Yibo Wu, Evan G. Williams, Ruedi Aebersold

引用次数: 10

Monitoring Pathogen-Induced Sickness in Mice and Rats 小鼠和大鼠病原性疾病的监测

Current protocols in mouse biology Pub Date : 2017-06-19 DOI: 10.1002/cpmo.27

Daria Kolmogorova, Emma Murray, Nafissa Ismail

{"title":"Monitoring Pathogen-Induced Sickness in Mice and Rats","authors":"Daria Kolmogorova, Emma Murray, Nafissa Ismail","doi":"10.1002/cpmo.27","DOIUrl":"10.1002/cpmo.27","url":null,"abstract":"Sickness behavior monitoring, a technique for examining the development of sickness symptomatology following infection, is necessary in experiments studying neurochemical and physiological changes associated with pathogen-induced immune activation. However, the results of sickness behavior monitoring are difficult to reconcile due to inconsistencies in protocol methods and rater bias. The protocol described herein offers a non-invasive and unbiased approach to assess the progression of pathogen-induced sickness behaviors. This simple, straightforward method uses a five-point scale to assess animals for the presence of four sickness behaviors (i.e., ‘“0” = no sickness behaviors; “4” = four sickness behaviors) at various time points following exposure to a pathogen. This approach removes the ambiguity and bias inherent to other methods of sickness behavior monitoring that rely on subjective ratings of severity for individual symptoms. This protocol has been successfully applied to male and female rodents injected intraperitoneally with lipopolysaccharide and polyinosinic:polycytidylic acid, and has been effective in pubertal and adult populations. Protocols for changes in body temperature and weight are also provided as physiological markers of sickness. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.27","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35101796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse 一种快速、简便、可定制的八色流式细胞术方法用于分析小鼠支气管肺泡灌洗液的细胞含量

Current protocols in mouse biology Pub Date : 2017-06-19 DOI: 10.1002/cpmo.26

François Daubeuf, Julien Becker, Juan Antonio Aguilar-Pimentel, Claudine Ebel, Martin Hrabě de Angelis, Yann Hérault, Nelly Frossard

{"title":"A Fast, Easy, and Customizable Eight-Color Flow Cytometric Method for Analysis of the Cellular Content of Bronchoalveolar Lavage Fluid in the Mouse","authors":"François Daubeuf, Julien Becker, Juan Antonio Aguilar-Pimentel, Claudine Ebel, Martin Hrabě de Angelis, Yann Hérault, Nelly Frossard","doi":"10.1002/cpmo.26","DOIUrl":"10.1002/cpmo.26","url":null,"abstract":"The cell composition of bronchoalveolar lavage fluid (BAL) is an important indicator of airway inflammation. It is commonly determined by cytocentrifuging leukocytes on slides, then staining, identifying, and counting them as eosinophils, neutrophils, macrophages, or lymphocytes according to morphological criteria under light microscopy, where it is not always easy to distinguish macrophages from lymphocytes. We describe here a one-step, easy-to-use, and easy-to-customize 8-color flow cytometric method for performing differential cell count and comparing it to morphological counts on stained cytospins. This method identifies BAL cells by a simultaneous one-step immunolabeling procedure using antibodies to identify T cells, B cells, neutrophils, eosinophils, and macrophages. Morphological analysis of flow-sorted cell subsets is used to validate this protocol. An important advantage of this basic flow cytometry protocol is the ability to customize it by the addition of antibodies to study receptor expression at leukocyte cell surfaces and identify subclasses of inflammatory cells as needed. © 2017 by John Wiley & Sons, Inc.","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":"7 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35100249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Analysis of mtDNA/nDNA Ratio in Mice 小鼠mtDNA/nDNA比值分析

Current protocols in mouse biology Pub Date : 2017-06-19 DOI: 10.1002/cpmo.21

Pedro M. Quiros, Aashima Goyal, Pooja Jha, Johan Auwerx

引用次数: 212