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Current protocols in mouse biology最新文献

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Viral Vectors for Gene Transfer 基因转移的病毒载体
Current protocols in mouse biology Pub Date : 2018-11-28 DOI: 10.1002/cpmo.58
Yong Hong Chen, Megan S. Keiser, Beverly L. Davidson
{"title":"Viral Vectors for Gene Transfer","authors":"Yong Hong Chen,&nbsp;Megan S. Keiser,&nbsp;Beverly L. Davidson","doi":"10.1002/cpmo.58","DOIUrl":"10.1002/cpmo.58","url":null,"abstract":"<p>Viral vectors are a promising tool for effective delivery of genetic material into cells. They take advantage of the natural ability of a virus to deliver a genetic payload into cells while being genetically modified such that their ability to replicate is crippled or removed. Here, an updated overview of routinely used viral vectors, including adeno-associated viruses (AAV), retroviruses/lentiviruses, and adenoviruses (Ads), is provided, as well as perspectives on their advantages and disadvantages in research and gene therapy. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.58","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36713616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 57
Techniques for Intracranial Stereotaxic Injections of Adeno-Associated Viral Vectors in Adult Mice 成年小鼠颅内立体定向注射腺相关病毒载体技术
Current protocols in mouse biology Pub Date : 2018-11-05 DOI: 10.1002/cpmo.57
Megan S. Keiser, Yong Hong Chen, Beverly L. Davidson
{"title":"Techniques for Intracranial Stereotaxic Injections of Adeno-Associated Viral Vectors in Adult Mice","authors":"Megan S. Keiser,&nbsp;Yong Hong Chen,&nbsp;Beverly L. Davidson","doi":"10.1002/cpmo.57","DOIUrl":"10.1002/cpmo.57","url":null,"abstract":"<p>Stereotaxic intracranial injection of viral vectors is a valuable technique to directly deliver genetic material to a specific population of cells in the central nervous system of a mouse model. This enables scientists to test candidate gene therapies or disease modulators that can then provide insight into the pathological mechanisms of disease. In this article, we present a standardized method of conducting intracranial stereotaxic injection of adeno-associated virus into a specific brain region in a mouse model. Support protocols are provided for virus dialysis and testing new stereotaxic coordinates with dye. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.57","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36648443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Studying Osteoarthritis Pathogenesis in Mice 小鼠骨关节炎发病机制的研究
Current protocols in mouse biology Pub Date : 2018-09-21 DOI: 10.1002/cpmo.50
Andrew Blease, Patricia Das Neves Borges, Marcia Curtinha, Behzad Javaheri, Isabell S. von Loga, Ida Parisi, Jadwiga Zarebska, Andrew Pitsillides, Tonia L. Vincent, Paul K. Potter
{"title":"Studying Osteoarthritis Pathogenesis in Mice","authors":"Andrew Blease,&nbsp;Patricia Das Neves Borges,&nbsp;Marcia Curtinha,&nbsp;Behzad Javaheri,&nbsp;Isabell S. von Loga,&nbsp;Ida Parisi,&nbsp;Jadwiga Zarebska,&nbsp;Andrew Pitsillides,&nbsp;Tonia L. Vincent,&nbsp;Paul K. Potter","doi":"10.1002/cpmo.50","DOIUrl":"10.1002/cpmo.50","url":null,"abstract":"<p>With the increasing availability and complexity of mouse models of disease, either spontaneous or induced, there is a concomitant increase in their use in the analysis of pathogenesis. Among such diseases is osteoarthritis, a debilitating disease with few treatment options. While advances in our understanding of the pathogenesis of osteoarthritis has advanced through clinical investigations and genome-wide association studies, there is still a large gap in our knowledge, hindering advances in therapy. Patient samples are available ex vivo, but these are generally in the very late stages of disease. However, with mice, we are able to induce disease at a defined time and track the progression in vivo and ex vivo, from inception to end stage, to delineate the processes involved in disease development. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.50","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36510725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Critical Assessment of the mdx Mouse with Ex Vivo Eccentric Contraction of the Diaphragm Muscle mdx小鼠离体膈肌偏心收缩的临界评估
Current protocols in mouse biology Pub Date : 2018-08-14 DOI: 10.1002/cpmo.49
Gregory C. Addicks, Phillip Marshall, Bernard J. Jasmin, Jean-Marc Renaud, Hongbo Zhang, Keir J. Menzies
{"title":"Critical Assessment of the mdx Mouse with Ex Vivo Eccentric Contraction of the Diaphragm Muscle","authors":"Gregory C. Addicks,&nbsp;Phillip Marshall,&nbsp;Bernard J. Jasmin,&nbsp;Jean-Marc Renaud,&nbsp;Hongbo Zhang,&nbsp;Keir J. Menzies","doi":"10.1002/cpmo.49","DOIUrl":"10.1002/cpmo.49","url":null,"abstract":"<p>Muscle function and health progressively deteriorate during the progression of muscle dystrophies. The ability to objectively characterize muscle function and muscle damage is useful not only when comparing variants of dystrophy models, but also for characterizing the effects of interventions aiming to improve or halt the progressive decline of muscle function and muscle health. The protocols in this chapter describe the use of <i>ex vivo</i> eccentric contraction of the diaphragm muscle as a measure of muscle susceptibility to damage. Because muscle has a robust regenerative capacity, unhealthy muscle may be functionally close to normal; therefore, protocols for <i>ex vivo</i> characterization of muscle are often essential for assessing the effects of interventions. Additional methods that can be applied for assessment of dystrophic muscle are also highlighted. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.49","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36394357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Muscle Stem Cell Immunostaining 肌肉干细胞免疫染色
Current protocols in mouse biology Pub Date : 2018-08-14 DOI: 10.1002/cpmo.47
Shuaiyu Wang, Bao Zhang, Gregory C. Addicks, Hui Zhang, Keir J. Menzies, Hongbo Zhang
{"title":"Muscle Stem Cell Immunostaining","authors":"Shuaiyu Wang,&nbsp;Bao Zhang,&nbsp;Gregory C. Addicks,&nbsp;Hui Zhang,&nbsp;Keir J. Menzies,&nbsp;Hongbo Zhang","doi":"10.1002/cpmo.47","DOIUrl":"10.1002/cpmo.47","url":null,"abstract":"<p>Muscle stem cells (MuSCs) are essential for maintaining muscle homeostasis by providing progenitor cells for muscle regeneration after injury and in muscular diseases. MuSC properties dynamically change, reflecting physiology or pathological status. For instance, MuSCs are activated after muscle injury, but become exhausted in late stages of Duchenne Muscular Dystrophy (DMD) disease and senescent during aging. Therefore, characterization of MuSCs, including proliferation, activation, senescence, and apoptosis, etc., is very important in applying MuSC knowledge to regenerative medicine, such as in the treatment of DMD and to improve muscle function in aging. Here, we describe a detailed method for characterizing MuSCs <i>in situ</i> using immunostaining techniques in the mouse. This method can also be easily adapted to analyze other skeletal muscle properties. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.47","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36396049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
A Method for Parasagittal Sectioning for Neuroanatomical Quantification of Brain Structures in the Adult Mouse 一种用于成年小鼠脑结构神经解剖定量的旁矢状面切片方法
Current protocols in mouse biology Pub Date : 2018-06-26 DOI: 10.1002/cpmo.48
Stephan C. Collins, Christel Wagner, Léo Gagliardi, Perrine F. Kretz, Marie-Christine Fischer, Pascal Kessler, Meghna Kannan, Binnaz Yalcin
{"title":"A Method for Parasagittal Sectioning for Neuroanatomical Quantification of Brain Structures in the Adult Mouse","authors":"Stephan C. Collins,&nbsp;Christel Wagner,&nbsp;Léo Gagliardi,&nbsp;Perrine F. Kretz,&nbsp;Marie-Christine Fischer,&nbsp;Pascal Kessler,&nbsp;Meghna Kannan,&nbsp;Binnaz Yalcin","doi":"10.1002/cpmo.48","DOIUrl":"10.1002/cpmo.48","url":null,"abstract":"<p>In this article, we present a standardized protocol for fast and robust neuroanatomical phenotyping of the adult mouse brain, which complements a previously published article (doi: 10.1002/cpmo.12) in <i>Current Protocols in Mouse Biology</i>. It is aimed at providing an experimental pipeline within an academic research setting from experimental work to data analysis. Our analysis focuses on one single parasagittal plane, covering the majority of brain regions involved in higher order cognitions such as the cortex, hippocampus, and cerebellum, for a total of 166 parameters of area, length, and cell-level measurements in contrast to 78 parameters in our previously published coronal screen. Benefits of using parasagittal analysis for large-scale neuroanatomic screens are discussed. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.48","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36259058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
Assessing Healthspan and Lifespan Measures in Aging Mice: Optimization of Testing Protocols, Replicability, and Rater Reliability 评估衰老小鼠的健康寿命和寿命:测试方案的优化、可重复性和更高的可靠性
Current protocols in mouse biology Pub Date : 2018-06-20 DOI: 10.1002/cpmo.45
Stacey J. Sukoff Rizzo, Laura C. Anderson, Torrian L. Green, Tracy McGarr, Gaylynn Wells, Shawn S. Winter
{"title":"Assessing Healthspan and Lifespan Measures in Aging Mice: Optimization of Testing Protocols, Replicability, and Rater Reliability","authors":"Stacey J. Sukoff Rizzo,&nbsp;Laura C. Anderson,&nbsp;Torrian L. Green,&nbsp;Tracy McGarr,&nbsp;Gaylynn Wells,&nbsp;Shawn S. Winter","doi":"10.1002/cpmo.45","DOIUrl":"10.1002/cpmo.45","url":null,"abstract":"<p>The relationship between chronological age (lifespan) and biological age (healthspan) varies amongst individuals. Understanding the normal trajectory and characteristic traits of aging mice throughout their lifespan is important for selecting the most reliable and reproducible measures to test hypotheses. The protocols herein describe assays used for aging studies at The Jackson Laboratory's Mouse Neurobehavioral Phenotyping Facility and include assessments of frailty, cognition, and sensory (hearing, vision, olfaction), motor, and fine motor function that can be used for assessing phenotypes in aged mice across their lifespan as well as provide guidance for setting up and validating these behavioral measures. Researchers aiming to study aging phenotypes require access to aged mice as a reference when initiating these types of studies in order to observe normal aging characteristics that cannot be observed in young adult mouse populations. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.45","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36241838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 45
Issue Information TOC 问题信息TOC
Current protocols in mouse biology Pub Date : 2018-06-01 DOI: 10.1111/ilrs.12051
{"title":"Issue Information TOC","authors":"","doi":"10.1111/ilrs.12051","DOIUrl":"https://doi.org/10.1111/ilrs.12051","url":null,"abstract":"","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/ilrs.12051","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48134849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Topographic Memory in Mice in a Complex Environment Using the Hamlet Test 用哈姆雷特测验评估复杂环境下小鼠地形记忆
Current protocols in mouse biology Pub Date : 2018-05-25 DOI: 10.1002/cpmo.43
Lucie Crouzier, Tangui Maurice
{"title":"Assessment of Topographic Memory in Mice in a Complex Environment Using the Hamlet Test","authors":"Lucie Crouzier,&nbsp;Tangui Maurice","doi":"10.1002/cpmo.43","DOIUrl":"10.1002/cpmo.43","url":null,"abstract":"<p>Here we provide instructions to measure topographic memory in mice using the Hamlet test, a complex environment. The apparatus mimics a small hamlet with a central agora and five houses, which are functionalized since mice can drink, eat pellets, hide within a small maze, run in an activity wheel, or interact with a stranger mouse behind a grid. The houses are interconnected through a network of streets in a five-arm star shape, and a video tracking system takes information from the activity in each house or follows a single mouse by trajectometry. Training the mice in the Hamlet, in groups for several hours per day over several days or weeks, allows consolidation of topographic memory (i.e., route learning involving both allocentric and egocentric strategies). Analysis of topographic memory can be performed a posteriori in a probe test by depriving mice of water or food and measuring their ability to efficiently reach the “eat” or “drink” house. Control groups include mice tested in non-deprived condition and mice naïve to the Hamlet and tested in deprived or non-deprived conditions. The present article details the apparatus, procedures, and protocols that can be used to reliably habituate mice in this complex environment and measure topographic memory. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.43","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36244441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
MRI to Assess Neurological Function MRI评估神经功能
Current protocols in mouse biology Pub Date : 2018-05-22 DOI: 10.1002/cpmo.44
Brian J. Nieman, Matthijs C. van Eede, Shoshana Spring, Jun Dazai, R. Mark Henkelman, Jason P. Lerch
{"title":"MRI to Assess Neurological Function","authors":"Brian J. Nieman,&nbsp;Matthijs C. van Eede,&nbsp;Shoshana Spring,&nbsp;Jun Dazai,&nbsp;R. Mark Henkelman,&nbsp;Jason P. Lerch","doi":"10.1002/cpmo.44","DOIUrl":"10.1002/cpmo.44","url":null,"abstract":"<p>This article describes a detailed set of protocols for mouse brain imaging using MRI. We focus primarily on measuring changes in neuroanatomy, and provide both instructions for mouse preparation and details on image acquisition, image processing, and statistics. Practical details as well as theoretical considerations are provided. © 2018 by John Wiley &amp; Sons, Inc.</p>","PeriodicalId":37980,"journal":{"name":"Current protocols in mouse biology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmo.44","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36244439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
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