Studying Osteoarthritis Pathogenesis in Mice

Q1 Agricultural and Biological Sciences

Current protocols in mouse biology Pub Date : 2018-09-21 DOI:10.1002/cpmo.50

Andrew Blease, Patricia Das Neves Borges, Marcia Curtinha, Behzad Javaheri, Isabell S. von Loga, Ida Parisi, Jadwiga Zarebska, Andrew Pitsillides, Tonia L. Vincent, Paul K. Potter

引用次数: 6

Abstract

With the increasing availability and complexity of mouse models of disease, either spontaneous or induced, there is a concomitant increase in their use in the analysis of pathogenesis. Among such diseases is osteoarthritis, a debilitating disease with few treatment options. While advances in our understanding of the pathogenesis of osteoarthritis has advanced through clinical investigations and genome-wide association studies, there is still a large gap in our knowledge, hindering advances in therapy. Patient samples are available ex vivo, but these are generally in the very late stages of disease. However, with mice, we are able to induce disease at a defined time and track the progression in vivo and ex vivo, from inception to end stage, to delineate the processes involved in disease development. © 2018 by John Wiley & Sons, Inc.

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小鼠骨关节炎发病机制的研究

随着疾病小鼠模型(自发或诱导)的可用性和复杂性的增加，它们在发病机制分析中的应用也随之增加。其中一种疾病是骨关节炎，这是一种使人衰弱的疾病，治疗方法很少。虽然通过临床研究和全基因组关联研究，我们对骨关节炎发病机制的了解有所进展，但我们的知识仍存在很大差距，阻碍了治疗的进展。患者的体外样本是可用的，但这些样本通常是在疾病的非常晚期。然而，对于小鼠，我们能够在确定的时间内诱导疾病，并跟踪体内和体外的进展，从开始到结束阶段，描绘疾病发展的过程。©2018 by John Wiley &儿子,Inc。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current protocols in mouse biology Agricultural and Biological Sciences-Animal Science and Zoology

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期刊介绍： Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment''s success are not captured in the primary literature but exist only as part of a lab''s oral tradition. The aim of Current Protocols in Mouse Biology is to provide the clearest, most detailed and reliable step-by-step instructions for protocols involving the use of mice in biomedical research. Written by experts in the field and extensively edited to our exacting standards, the protocols include all of the information necessary to complete an experiment in the laboratory—introduction, materials lists with supplier information, detailed step-by-step procedures with helpful annotations, recipes for reagents and solutions, illustrative figures and information-packed tables. Each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, time considerations, and tips to help avoid common pitfalls and troubleshoot experiments. Furthermore, Current Protocols in Mouse Biology content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly issues allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments. Current Protocols in Mouse Biology brings together resources in mouse biology and genetics and provides a mouse protocol resource that covers all aspects of mouse biology. Current Protocols in Mouse Biology also permits optimization of mouse model usage, which is significantly impacted by both cost and ethical constraints. Optimal and standardized mouse protocols ultimately reduce experimental variability and reduce the number of animals used in mouse experiments.