Bioprinting最新文献

{"title":"AI-driven closed-loop extrusion-based bioprinting system: from dynamic regulation to medical translation","authors":"Dongxu Ke","doi":"10.1016/j.bprint.2025.e00459","DOIUrl":"10.1016/j.bprint.2025.e00459","url":null,"abstract":"<div><div>Extrusion-based bioprinting (EBB) has emerged as a pivotal technology in tissue engineering, enabling the fabrication of complex, cell-laden constructs through precise deposition of bioinks. Recent advancements integrate artificial intelligence (AI) with closed-loop control systems to address critical challenges in reproducibility, resolution, and scalability. This review highlights the synergistic convergence of AI-driven methodologies and dynamic feedback mechanisms in revolutionizing EBB. Key innovations include machine learning (ML)-enabled predictive models for bioink formulation optimization, intelligent scaffold design, and real-time quality control. Despite progress, challenges persist in model generalizability, requiring extensive datasets across laboratory-to-industrial scales to ensure translational robustness. Future research must prioritize scalable AI architectures, cross-disciplinary validation, and regulatory-compliant workflows to accelerate the clinical adoption of intelligent biomanufacturing platforms. This paradigm shift toward adaptive, self-optimizing EBB systems holds transformative potential for regenerative medicine, drug screening, and organ-on-chip technologies.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"54 ","pages":"Article e00459"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlating linear rheology and filament fidelity in bioinks: A combined modeling and experimental approach","authors":"Ahasan Habib ,&nbsp;Rokeya Sarah ,&nbsp;Larry A. Villasmil-Urdaneta ,&nbsp;Michael Cromer","doi":"10.1016/j.bprint.2026.e00471","DOIUrl":"10.1016/j.bprint.2026.e00471","url":null,"abstract":"<div><div>The rheological behavior of bioinks critically influences their printability, yet current models rely heavily on empirical fits that do not account for or ignore the underlying molecular mechanisms. In this study, we introduce a physically grounded transient network model that links polymer chain attachment–detachment kinetics to small-amplitude oscillatory shear (SAOS) moduli. By resolving chain populations across a spectrum of detachment rates, the model captures the power-law scaling of storage and loss moduli observed in alginate and carboxymethyl cellulose hydrogels. Unlike multimode Maxwell or fractional viscoelastic models, our framework assigns kinetic significance to each network relaxation mode, enabling mechanistic interpretation of viscoelastic behavior. Experimental rheology and filament printing tests further confirm that formulations with higher elastic moduli and lower loss tangents exhibit superior shape fidelity. The model fits SAOS data with high fidelity, replicating <span><math><msup><mrow><mi>G</mi></mrow><mrow><mo>′</mo></mrow></msup></math></span> and <span><math><msup><mrow><mi>G</mi></mrow><mrow><mo>′</mo><mo>′</mo></mrow></msup></math></span> (storage and loss moduli) within <span><math><mo>±</mo></math></span>2% across the analyzed frequency range under analysis, while yielding interpretable parameters such as chain stiffness, relaxation time, and reattachment rate. This work establishes a foundation for predictive bioink design, bridging molecular kinetics and macroscopic rheology in a way that supports rational formulation strategies for extrusion-based bioprinting.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"54 ","pages":"Article e00471"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed alginate/gelatin/PCL/hyaluronic acid scaffolds with metal–organic frameworks for quercetin delivery: Improving mechanical properties and cytocompatibility for cartilage tissue engineering applications","authors":"Tahoora Naseri ,&nbsp;Mohamadreza Soltani ,&nbsp;Samira Hosseinian ,&nbsp;Amirmohamad Alimohamadi ,&nbsp;Mohammad Soleimani ,&nbsp;Amir Shamloo ,&nbsp;Shohreh Mashayekhan","doi":"10.1016/j.bprint.2026.e00466","DOIUrl":"10.1016/j.bprint.2026.e00466","url":null,"abstract":"<div><div>Designing biocompatible scaffold for cartilage tissue engineering applications requires creating an environment that supports cell viability while withstanding mechanical stress. In this study, a novel hybrid scaffold composed of 3D printed hydrogel scaffold with hydrogel ink composed of Alginate, Gelatin, and Hyaluronic acid, incorporated with 3D printed PCL scaffold, was designed, fabricated, and their characteristics were assessed. The novelty of this design is to achieve favorable porous, biocompatible environment with higher mechanical strength (<span><math><mrow><mn>20.872</mn><mo>±</mo><mn>0.271</mn></mrow></math></span> MPa Young's modulus) compared to cartilage tissue deep zone (<span><math><mrow><mn>12.06</mn><mspace></mspace><mo>±</mo><mspace></mspace><mn>1.03</mn></mrow></math></span> MPa), that exhibits proper chondrocyte <span><math><mrow><mn>120.83</mn><mo>%</mo></mrow></math></span>, cell viability 24 h after incubation. ZIF-8 and UIO-66 metal-organic framework (MOF) nanoparticles were used as carriers for anti-inflammatory quercetin drug to perform targeted drug delivery, by being loaded onto the hydrogel scaffold. High drug loading of ZIF-8 (36 %), and UIO-66 (15 %), along with a controlled degradation rate (48.64 %, after 7 days), prevented burst release of the quercetin drug. The higher drug release of ZIF-8 leads to higher inhibition zone against <em>S. aureus</em> (<span><math><mrow><mn>36</mn><mspace></mspace><mo>±</mo><mspace></mspace><mn>0.5</mn></mrow></math></span> mm). Moreover, higher drug loading of ZIF-8 exhibited better C28 cell viability compared to UIO-66 (<span><math><mrow><mn>317</mn><mo>%</mo></mrow></math></span> cell viability for Que@ZIF-8, 24 h after incubation). These results demonstrate that the composition of proper mechanical strength of the scaffold integrated with a smart drug delivery system using MOFs indicates scaffold's potential for cartilage tissue engineering applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"54 ","pages":"Article e00466"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human umbilical cord-derived dECM-Chitosan hybrid biomaterial ink for 3D bioprinting applications","authors":"Tayyaba Bari ,&nbsp;Asma Tufail Shah ,&nbsp;Anila Asif ,&nbsp;Rahat Naseer ,&nbsp;Azmat Ullah Khan ,&nbsp;Fakhera Ikram ,&nbsp;Syed Qasim Raza","doi":"10.1016/j.bprint.2026.e00465","DOIUrl":"10.1016/j.bprint.2026.e00465","url":null,"abstract":"<div><div>Decellularized extracellular matrix (dECM) based materials are increasingly explored in biofabrication due to their inherent bioactivity and tissue-specific cues; however, many reported systems rely on synthetic or photo-crosslinkable components to achieve printability.</div><div>In this study, we present a human umbilical cord derived dECM–chitosan composite as a potential bioink material, ionically crosslinked using β-glycerol phosphate (BGP) and sodium hydrogen carbonate (SHC) under physiological conditions for extrusion based 3D printing. The formulation demonstrated shear-thinning behavior, rapid gelation, and stable acellular extrusion, enabling the fabrication of three-dimensional bioscaffolds with interconnected porosity (∼500 μm) and preserved structural fidelity. The printed constructs exhibited a semi-crystalline architecture and compressive properties (≈1.7 MPa, 53 % strain at break) within the lower range of native tissues. In addition, the bioscaffolds displayed antibacterial activity against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>, supporting their multifunctional character. The Alamar Blue assay performed on surface-seeded NIH3T3 fibroblasts demonstrated sustained metabolic activity and high cytocompatibility (&gt;95 % viability) over a seven-day culture period. These findings confirm the material's printability, mechanical integrity, and biological compatibility at the bioscaffold level; however, further studies are required to validate its performance as a cell-laden bioink.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"54 ","pages":"Article e00465"},"PeriodicalIF":0.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and characterization of 3D printed polycaprolactone/pluronic F-127 scaffold incorporating ciprofloxacin-loaded halloysite nanotubes to promote in vivo bone regeneration","authors":"Seyedeh Mohadeseh Ghoreyshi ,&nbsp;Azadeh Ghaee ,&nbsp;Esmaeil Mirzaei ,&nbsp;Sarah Hosseinidoust","doi":"10.1016/j.bprint.2025.e00462","DOIUrl":"10.1016/j.bprint.2025.e00462","url":null,"abstract":"<div><div>This study fabricated a 3D-printed nanocomposite scaffold for enhanced bone regeneration based on biocompatible synthetic polymers. Polycaprolactone (PCL) was combined with Pluronic F-127 (PF-127) to improve hydrophilicity, and halloysite nanotubes (HNTs) loaded with ciprofloxacin (CPX) were incorporated to enhance the scaffold's mechanical, biological, and antibacterial properties. The resulting scaffolds exhibited interconnected pores and optimal porosity (50 %), crucial for cell migration and vascularization. FTIR and XRD confirmed the integration of PF-127 and HNTs-CPX within the PCL matrix. Incorporation of PF-127 significantly enhanced water uptake, while HNTs improved mechanical strength. The presence of CPX provided effective antibacterial activity against <em>E. coli</em> and <em>S. aureus</em>. In vitro assays with MG-63 cells demonstrated enhanced cell adhesion, proliferation, and osteogenic differentiation (ALP activity, Alizarin Red S staining) on HNTs-containing scaffolds. In vivo studies revealed that the scaffold contains 3 % of HNTs-CPX promoted bone regeneration, characterized by increased collagen synthesis and neovascularization, compared to pure PCL scaffold. These findings highlight the potential of this 3D-printed nanocomposite scaffold and offer a promising solution for effective bone tissue engineering applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"53 ","pages":"Article e00462"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering the future of organ transplantation: A comprehensive review of 3D bioprinting advances for organ bioengineering","authors":"Ryan Idnani ,&nbsp;Sahil Khan","doi":"10.1016/j.bprint.2025.e00463","DOIUrl":"10.1016/j.bprint.2025.e00463","url":null,"abstract":"<div><div>3D bioprinting represents a transformative convergence of engineering and biology, enabling the precise, layer-by-layer fabrication of complex, functional tissues and organs, with the potential to solve the global organ shortage crisis. This literature review synthesizes recent advancements across core bioprinting modalities and innovative bioinks, while integrating quantitative physiological constraints, vascularization strategies, AI, microfluidics, and translational manufacturing considerations into a unified design framework. The foundational mechanisms of 3D bioprinting (inkjet, extrusion-based, laser-assisted, stereolithography) balance resolution and cell viability alongside next-generation bioinks (decellularized ECM, hybrid nanocomposites and functionalized hydrogels) for mechanical reinforcement, bioactivity and controlled cell behavior. Quantitative performance benchmarks (cell density, diffusion limits, shear stress ranges and vascular architecture requirements) are introduced to contextualize material and hardware choices for organ-scale fabrication. This technology has continued to evolve with the innovation of multimodal printing systems, 4D bioprinting and high-throughput platforms. This is supported by emerging AI-enabled workflows for bioink optimization, print-path planning, real-time process control and quality assurance. Organ-specific applications for liver, heart, kidney and skin illustrate the field's translational potential for fabricating organ replacements. Beyond experimental demonstrations, this review situates bioprinting within its broader translational ecosystem, addressing scalability, vascular integration, immune considerations, regulatory standardization and GMP-compatible production. Despite significant progress, challenges in scalability, vascularization and regulatory standardization remain. By articulating quantitative design rules and a systems-level roadmap, this review moves beyond descriptive surveys to define actionable principles for advancing bioprinted tissues towards clinically deployable, transplant-ready organs.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"53 ","pages":"Article e00463"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145841985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed PLGA/PLA scaffold with PEGylated gold nanorods and doxorubicin for dual-mode bone cancer therapy","authors":"Mahsa Mohammadzadeh ,&nbsp;Sheyda Labbaf ,&nbsp;Ahmad Kermanpur ,&nbsp;Javad Esmaeili","doi":"10.1016/j.bprint.2025.e00460","DOIUrl":"10.1016/j.bprint.2025.e00460","url":null,"abstract":"<div><div>Osteosarcoma, a highly aggressive bone malignancy affecting children and adolescents, remains difficult to treat due to drug resistance, systemic toxicity, and high recurrence rates. This study introduces a multifunctional 3D-printed PLGA/PLA scaffold incorporating PEGylated gold nanorods (GNRs@PEG) loaded with doxorubicin (DOX) to enable combined photothermal-chemotherapeutic treatment. Two scaffold formulations were investigated: Target-1 scaffold, consisting of the PLGA/PLA scaffold with uniformly dispersed GNRs@PEG-DOX, and Target-2 scaffold, which includes an additional chitosan (CS) hydrogel coating to enhance hydrophilicity and biological interactions. Target-1 scaffold exhibited increased surface roughness and a compressive strength of 57 MPa, whereas Target-2 scaffold demonstrated higher water uptake and improved flexibility due to the CS layer. Under 810 nm NIR irradiation, both scaffolds efficiently converted light to heat (≈48–51 °C), enabling on-demand DOX release and inducing apoptosis in MG63 osteosarcoma cells. The combined PTT + DOX treatment reduced MG63 viability to below 20 %, indicating a pronounced therapeutic enhancement. These results highlight the potential of GNR-integrated 3D-printed scaffolds as promising platforms for localized osteosarcoma management.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"53 ","pages":"Article e00460"},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145753865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Physics of cellular self-assembly– a microscopic model and mathematical framework for faster maturation of bioprinted tissues” [Bioprinting (14) (2019) 47]","authors":"Ashkan Shafiee ,&nbsp;Elham Ghadiri ,&nbsp;David Williams ,&nbsp;Anthony Atala","doi":"10.1016/j.bprint.2025.e00448","DOIUrl":"10.1016/j.bprint.2025.e00448","url":null,"abstract":"","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"52 ","pages":"Article e00448"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of process parameters for fabricating PLA/Mg/HA biocomposite filaments using the Taguchi method for biomedical applications","authors":"Akbar Teguh Prakoso ,&nbsp;Hasan Basri ,&nbsp;Amir Arifin ,&nbsp;Gunawan ,&nbsp;Dendy Adanta ,&nbsp;Imam Akbar ,&nbsp;Ardiyansyah Syahrom","doi":"10.1016/j.bprint.2025.e00451","DOIUrl":"10.1016/j.bprint.2025.e00451","url":null,"abstract":"<div><div>This paper aims to optimize the single screw extrusion (SSE) process parameters for the production of polylactic acid (PLA)/magnesium (Mg)/hydroxyapatite (HA) composite filaments, with the goal of achieving a final filament diameter of 1.75 mm to improve process efficiency and output quality. The Taguchi method was utilized to systematically assess the influence of screw speed, nozzle diameter, material composition, and temperature on filament diameter. The findings revealed that screw speed, nozzle diameter, material composition, and temperature significantly impacted the filament diameter. Under optimal conditions (94 % wt PLA, 4 % wt Mg, and 2 % wt HA, 1.95 mm nozzle diameter, 6 rpm screw speed, and 175 °C), the filament diameter approached 1.75 mm. This study contributes to enhancing the production efficiency and quality of composite filaments, with potential applications in advanced bone scaffold fabrication, although additional research is needed for clinical application confirmation.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"52 ","pages":"Article e00451"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145569419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “3D bioprinted biomask for facial skin reconstruction” [Bioprinting 10 (2018) 28]","authors":"Young-Joon Seol ,&nbsp;Hyungseok Lee ,&nbsp;Joshua S. Copus ,&nbsp;Hyun-Wook Kang ,&nbsp;Dong-Woo Cho ,&nbsp;Anthony Atala ,&nbsp;Sang Jin Lee ,&nbsp;James J. Yoo","doi":"10.1016/j.bprint.2025.e00450","DOIUrl":"10.1016/j.bprint.2025.e00450","url":null,"abstract":"","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"52 ","pages":"Article e00450"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145736336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}