Bioprinting最新文献_第10页

Thermoplastic polycaprolactone elastomer for a 3D-printed pericardial scaffold in the treatment of dilated cardiomyopathy 热塑性聚己内酯弹性体用于3d打印心包支架治疗扩张型心肌病

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00252

David McCoul, Wei Nie, Phillip Kim, Carlos Kengla, Cara Clouse, Anthony Atala

{"title":"Thermoplastic polycaprolactone elastomer for a 3D-printed pericardial scaffold in the treatment of dilated cardiomyopathy","authors":"David McCoul, Wei Nie, Phillip Kim, Carlos Kengla, Cara Clouse, Anthony Atala","doi":"10.1016/j.bprint.2022.e00252","DOIUrl":"10.1016/j.bprint.2022.e00252","url":null,"abstract":"<div>A novel thermoplastic polycaprolactone-based elastomer (TPE) has been developed for a fused deposition modeling (FDM) 3D-printed pericardial scaffold aimed at the treatment of dilated cardiomyopathy (DCM). The TPE polymer was synthesized from polycaprolactone (PCL) diol, hexamethylene diisocyanate, Tin(II) 2-ethylhexanoate, and 1,4-butanediol, then purified via rotary evaporation and vacuum drying. Biocompatibility was verified using a cell counting kit (CCK-8) with NIH/3T3 cells. Tensile mechanical properties were quantified, and the TPE was reliable for more than 104 cycles, and up to 107 cycles in Dulbecco's phosphate-buffered saline (DPBS) heated to 37 °C. Its lack of chemical crosslinking allows it to remain a thermoplastic, able to be melted and extruded for 3D printing at or above 120 °C using compressed nitrogen gas. Cardiac MRI scans of Sprague Dawley rats were converted to a 3D solid model, and a pericardial scaffold was designed and 3D printed for in vitro testing as a precursor to preclinical trials.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00252"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42289562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Corneal bioprinting using a high concentration pure collagen I transparent bioink 角膜生物打印采用高浓度纯胶原I透明生物墨水

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00235

Yihui Song , Sheng Hua , Sepidar Sayyar , Zhi Chen , Johnson Chung , Xiao Liu , Zhilian Yue , Cameron Angus , Benjamin Filippi , Stephen Beirne , Gordon Wallace , Gerard Sutton , Jingjing You

{"title":"Corneal bioprinting using a high concentration pure collagen I transparent bioink","authors":"Yihui Song , Sheng Hua , Sepidar Sayyar , Zhi Chen , Johnson Chung , Xiao Liu , Zhilian Yue , Cameron Angus , Benjamin Filippi , Stephen Beirne , Gordon Wallace , Gerard Sutton , Jingjing You","doi":"10.1016/j.bprint.2022.e00235","DOIUrl":"10.1016/j.bprint.2022.e00235","url":null,"abstract":"<div>The use of 3D printing to produce a bioengineered cornea is emerging as an approach to help alleviate the global shortage of donor corneas. Collagen Type 1 (Col-1) is the most abundant collagen in the human cornea. However, Col-I presents challenges as a bioink. It can self-assemble at neutral pH, making phase transitions as required for 3D printing difficult to control. Furthermore, low concentration solutions required for the transparency of printed Col-I lead to weak mechanical properties in its printed structures. In this study, Col-I at high concentrations, was tested with 15 different solutions to identify the composition preventing Col-I self-assembly. A stable Col-I bioink was then developed using riboflavin as a photoinitiator and UV irradiation-induced crosslinking. The mechanical properties and transparency, of the structures produced, were evaluated. The optimised Col-I bioink with corneal stromal cells was tested using a spiral printing method. The printed structure was transparent, and the encapsulated corneal stromal cells had over 90% viability after three weeks of culturing.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00235"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46354060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effect of varying cell densities on the rheological properties of the bioink 不同细胞密度对生物链流变特性的影响

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00241

Nilotpal Majumder, Aditya Mishra, Sourabh Ghosh

{"title":"Effect of varying cell densities on the rheological properties of the bioink","authors":"Nilotpal Majumder, Aditya Mishra, Sourabh Ghosh","doi":"10.1016/j.bprint.2022.e00241","DOIUrl":"10.1016/j.bprint.2022.e00241","url":null,"abstract":"<div>The rheological characterization of any biopolymer solution is crucial for evaluating the overall printability or injectability of the hydrogel. However, the effect of cells in the cell-laden hydrogel's rheological profile is often ignored. As a result, there is a significant difference in the predicted and experimental outcome in the structural stability of the construct as well as on the cell viability, proliferation, and differentiation potential of the embedded cells. Our present study has addressed the effect of different cell densities (0.1 million cells/ml, 0.5 million cells/ml, 1 million cells/ml and 2 million cells/ml) of TVA-BMSCs on the flow property, modulus behaviour, gelation kinetics and printability of our proprietary silk fibroin-gelatin (5SF-6G) bioink. The cell-laden hydrogels demonstrated a characteristic shear thinning behaviour (low initial viscosity), low storage modulus and increased gelation time when compared to the acellular 5SF-6G hydrogel. The printability analysis also portrayed a square pore geometry with low spreading ratio in 1 million cells/ml encapsulated 5SF-6G hydrogel comparable to the acellular hydrogel. We postulated that incorporation of cells in the bioink interfered with the gelation mechanism of the mushroom tyrosinase in the 5SF-6G bioink by masking the active sites. Additionally, the mechanistic crosstalk between the cell-surface integrins with the cell-attachment motifs of the biomaterial alters the cellular biomechanics of the cell that in-turn profoundly impacts the rheological properties of the polymer blend. Therefore, cell density of 1 million cells/ml was considered the best fit for extrusion-based 3D bioprinting owing to its optimum rheological traits and printability index akin the acellular hydrogel.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00241"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44098616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Three-dimensional (3D) printing of hydroxyapatite-based scaffolds: A review 羟基磷灰石基支架的三维打印研究进展

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00244

Mallikharjuna Reddy Bogala

引用次数: 4

Recent advances on cancer-on-chip models: Development of 3D tumors and tumor microenvironment 癌症芯片模型的最新进展：三维肿瘤和肿瘤微环境的发展

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00238

Nafiseh Moghimi , Seied Ali Hosseini , Mahla Poudineh , Mohammad Kohandel

{"title":"Recent advances on cancer-on-chip models: Development of 3D tumors and tumor microenvironment","authors":"Nafiseh Moghimi , Seied Ali Hosseini , Mahla Poudineh , Mohammad Kohandel","doi":"10.1016/j.bprint.2022.e00238","DOIUrl":"10.1016/j.bprint.2022.e00238","url":null,"abstract":"<div>Tumors are complex three-dimensional (3D) tissues that form in a microenvironment consisting of a heterogeneous mixture of cellular and non-cellular components. Due to the limitation of animal models, in vitro recreation of tumors has been developed for fundamental cancer studies and anticancer therapies. Compared to the 2D culture of cell monolayers, 3D-culture systems evidently show better recapitulation of architecture, tumor physiology, and cellular microenvironment. Furthermore, microfluidic devices provide better platforms to mimic the relevant cancerous features and the dynamic of tumor physiology which conventional 3D culture systems fail to recreate.A growing body of research has been published recently in the literature, which highlights the benefits of the 3D in vitro models in microfluidic devices. This review will provide an overview of the most recent 3D in vitro models, so-called tumor-on-chip systems, with emphasis on the fabrication of 3D culture systems and tumor microenvironment in microfluidic devices. First, the fabrication methods of 3D tumors are being described, where we present the conventional spheroid formation techniques and the novel 3D bioprinting approaches. Investigation of the steps that are involved in bioprinting is being presented, and different bioinks are introduced. In the second part, the tumor microenvironment (TME) and its role in cancer progression are being introduced. Different microfluidic technologies that are developed to stimulate multiple components of TME are studied, and their interactions with tumor cells are being discussed.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00238"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46801667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A bioprinted vascularized skin substitute with fibroblasts, keratinocytes, and endothelial progenitor cells for skin wound healing 一种生物打印的血管化皮肤替代品，含有成纤维细胞、角化细胞和内皮祖细胞，用于皮肤伤口愈合

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00237

Lien-Guo Dai , Niann-Tzyy Dai , Tsai-Yu Chen , Lan-Ya Kang , Shan-hui Hsu

{"title":"A bioprinted vascularized skin substitute with fibroblasts, keratinocytes, and endothelial progenitor cells for skin wound healing","authors":"Lien-Guo Dai , Niann-Tzyy Dai , Tsai-Yu Chen , Lan-Ya Kang , Shan-hui Hsu","doi":"10.1016/j.bprint.2022.e00237","DOIUrl":"10.1016/j.bprint.2022.e00237","url":null,"abstract":"<div>Skin substitutes are highly demanded by patients with extensive burns and full-thickness skin wounds. Bioprinting offers a promising technology to fabricate customized cell-laden skin substitutes. In this study, a hydrogel of biodegradable polyurethane (PU)-gelatin (4:1) laden with human fibroblasts, endothelial progenitor cells (EPCs), and keratinocytes was used as the bioink for building a bi-layer dermo-epidermal skin substitute. The seven-layer cell-laden constructs with stack thickness of 1.4 mm were precisely deposited through a 210 μm nozzle with an air pressure of 0.055–0.175 MPa and nozzle temperature of 19 °C. When grown in vitro, three types of cells in the bioprinted constructs showed good cell viability (>99%) in 24 h and reached a high proliferation rate (>220%) in 14 days. Significantly, EPCs were successfully differentiated into endothelial-like cells in the constructs and expressed the vasculogenesis-related proteins (CD31+ and eNOS+). When implanted in vivo, the bi-layer constructs attained ∼90% wound healing ratio and ∼76% re-epithelialization after 28 days in the nude mice model. Histological analyses revealed that skin wounds treated with the bi-layer constructs achieved high degrees of tissue integration and collagen production after 28 days. Vasculogenesis and angiogenesis of the wound treated with bi-layer constructs was significantly greater (∼300%) than those of the untreated wounds. The in vitro and in vivo findings indicate that the bioprinted skin substitutes with fibroblasts, EPCs, and keratinocytes embedded in PU-gelatin hydrogel may offer a promising strategy for clinical wound treatment and development of bioprinted skin.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00237"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42852962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Spatiotemporal T cell dynamics in a 3D bioprinted immunotherapy model 3D生物打印免疫治疗模型中的时空T细胞动力学

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00231

Cameron D. Morley , Catherine T. Flores , Jeffery A. Drake , Ginger L. Moore , Duane A. Mitchell , Thomas E. Angelini

{"title":"Spatiotemporal T cell dynamics in a 3D bioprinted immunotherapy model","authors":"Cameron D. Morley , Catherine T. Flores , Jeffery A. Drake , Ginger L. Moore , Duane A. Mitchell , Thomas E. Angelini","doi":"10.1016/j.bprint.2022.e00231","DOIUrl":"10.1016/j.bprint.2022.e00231","url":null,"abstract":"<div>3D bioprinting-focused research is often driven by the aspiration to manufacture functional tissues and organs for implantation, yet decades of additional research is likely needed to develop such technologies, broadly. By contrast, we currently have 3D bioprinting tools capable of generating precise, spatially defined distributions of different cell types that can be used in fundamental and applied research, without delay. Here, we demonstrate this capability by 3D printing adoptive immunotherapy models using KR158B cells (murine glioma), hematopoietic stem cells (HSCs), and tumor-reactive T cells. We leverage a recently developed material made from packed microgel particles that serves simultaneously as a 3D printing support material and a 3D culture medium. With this approach we create well-defined 3D cell distributions and investigate the interactions between the different cell populations with time-lapse confocal microscopy. We find that 3D printed tumor spheroids containing HSCs recruit T cells more rapidly than those lacking HSCs, where T cell motion appears to be guided by diffusing cytokines originating at the tumor spheroid surface. After the T cells interact with the glioma structures, we collect the different cell populations by running our 3D bioprinter in reverse and perform transcriptomic analysis. We find that the differences in gene expression, comparing tumor spheroids printed with and without HSCs, are consistent with those found in a murine model. These results establish a path forward for developing validated 3D printed models for pre-clinical testing.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00231"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41933876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

3D bioprinting for the repair of articular cartilage and osteochondral tissue 3D生物打印修复关节软骨和骨软骨组织

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00239

Nicholas A. Chartrain , Kristin H. Gilchrist , Vincent B. Ho , George J. Klarmann

{"title":"3D bioprinting for the repair of articular cartilage and osteochondral tissue","authors":"Nicholas A. Chartrain , Kristin H. Gilchrist , Vincent B. Ho , George J. Klarmann","doi":"10.1016/j.bprint.2022.e00239","DOIUrl":"10.1016/j.bprint.2022.e00239","url":null,"abstract":"<div>The poor intrinsic healing properties of cartilage result in the frequent development of osteoarthritis and chronic tissue degeneration following injury. Articular cartilage tissue engineering has produced several therapies, but the fabrication of functional and anatomically accurate engineered osteochondral tissue has proved elusive. 3D bioprinting's ability to fabricate complex tissue scaffolds that incorporate cells, extracellular matrix components, and growth factors enables engineered tissue that mimics the structure and function of natural tissue. Recent advances have allowed 3D bioprinting to emerge as a promising technology for the fabrication of tissue engineered osteochondral tissue suitable for the replacement of damaged cartilage. In this paper, we review pathologies, current treatments for osteochondral injuries, and recent advances in 3D bioprinting osteochondral tissue. Innovations in bioink formulation, bioprinting techniques, and results from preclinical studies are highlighted. Finally, we discuss the role that 3D bioprinting may have in the next generation of tissue engineered implants despite the substantial challenges that remain for the clinical translation of transplanted bioprinted osteochondral tissue, including the recapitulation of the complex zonal properties of cartilage, improving the mechanical properties of printed tissue scaffolds, and regulatory hurdles.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00239"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46970618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Motion compensation system for robotic based in situ bioprinting to balance patient physiological movements 基于原位生物打印的机器人运动补偿系统，以平衡患者的生理运动

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00248

Gabriele Maria Fortunato, Amedeo Franco Bonatti, Elisa Batoni, Ruggero Macaluso, Giovanni Vozzi, Carmelo De Maria

{"title":"Motion compensation system for robotic based in situ bioprinting to balance patient physiological movements","authors":"Gabriele Maria Fortunato, Amedeo Franco Bonatti, Elisa Batoni, Ruggero Macaluso, Giovanni Vozzi, Carmelo De Maria","doi":"10.1016/j.bprint.2022.e00248","DOIUrl":"10.1016/j.bprint.2022.e00248","url":null,"abstract":"<div>The aim of this study is to design and develop a robotic system capable of compensating a patient's periodic movement, such as a beating heart, breath-induced thoracic cavity motion, and able to avoid collisions in case of sudden and unexpected motions, caused by pain, tremor, or other diseases, during an in situ bioprinting process. Based on the previous work carried out on the IMAGObot platform (a 5 Degrees of Freedom robotic manipulator), the aim is to print on moving and non-planar surfaces, following the trajectory of a fiducial marker placed onto the patient, inside the robot workspace. For this purpose, a monocular vision system (featured by a webcam and fiducial markers positioned in the robot environment) and a software interface communicating with the robot controller were developed. The control algorithm was entirely developed in the Python environment using the OpenCV library for marker pose estimation and used to update the robot trajectory concerning the detected marker motion on LinuxCNC software. Moreover, in order to mimic the physiological displacement of a patient's rib cage due to breathing, a moving 3D-printed platform and a silicone chest phantom were fabricated. The motion compensation system was tested by regenerating a defect on the chest phantom during the respiratory phase through extrusion based in situ bioprinting.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00248"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43553042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

3D bioprinted silk-reinforced Alginate-Gellan Gum constructs for cartilage regeneration 3D生物打印丝增强海藻酸结冷胶构建软骨再生

Bioprinting Pub Date : 2022-12-01 DOI: 10.1016/j.bprint.2022.e00232

Juhi Chakraborty, Nilotpal Majumder, Aarushi Sharma, Sukanya Prasad, Sourabh Ghosh

{"title":"3D bioprinted silk-reinforced Alginate-Gellan Gum constructs for cartilage regeneration","authors":"Juhi Chakraborty, Nilotpal Majumder, Aarushi Sharma, Sukanya Prasad, Sourabh Ghosh","doi":"10.1016/j.bprint.2022.e00232","DOIUrl":"10.1016/j.bprint.2022.e00232","url":null,"abstract":"<div>Even though a substantial amount of research has been undertaken in the domains of bioprinting over the last few years, various challenges exist concerning printability. One of the grave challenges in developing a bioink with superior printability is the constraint of material availability, cross-linking, and other processing parameters that should warrant adequate functioning post bioprinting. This study demonstrates the development of a multicomponent shear-thinning bioink comprising Alginate and Gellan Gum with good mechanical, biological, and adequate printing properties. The addition of two types of silk nanoparticles (SNP), native and regenerated, acted as a reinforcement to the bioink, enhancing its printability. Additionally, silk fibroin solution was added to the bioink that served as a control apart from SNP. The addition of cationic SNP (native) to the anionic polymer mixture of Alginate-Gellan Gum exhibited a remarkable increase in the viscosity, storage modulus and mechanical property compared to the other experimental sets. Molecular characterization studies involving Real-Time PCR, gene expression, immunofluorescence, and histology analysis depicted enhanced chondrogenesis in the bioprinted construct containing the silk fibroin solution. The incorporated SNPs resulted in extracellular matrix secretion towards chondrogenesis of articular cartilage. Taken together, the method's broad applicability is likely to propel the field closer to the goal of precision bioprinting.</div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00232"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45400526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10