Bioprinting最新文献

{"title":"Adjusting degradation rate, mechanical properties and bioactivity of 3D-Printed biphasic calcium phosphate scaffolds by silk fibroin/ platelet-rich plasma lysate coating for regeneration of craniofacial bone defects","authors":"Samira Tajvar ,&nbsp;Afra Hadjizadeh ,&nbsp;Saeed Saber Samandari ,&nbsp;Shohreh Mashayekhan","doi":"10.1016/j.bprint.2025.e00389","DOIUrl":"10.1016/j.bprint.2025.e00389","url":null,"abstract":"<div><div>Despite many advances, reconstruction of craniofacial bone defects has faced many challenges due to their complex anatomy. For this purpose, in recent decades, researchers have focused on developing biomimetic and patient-specific engineered tissues. In this study, we developed scaffolds designed specifically for craniofacial bone defects, featuring optimal mechanical properties and degradation rates. To this end, porous scaffolds based on Na- and Mg-doped carbonated hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) were prepared using 3D printing. The printed scaffolds were then coated with silk fibroin (SF) and human platelet-rich plasma lysate (HPL). The degradation rate of the scaffolds was optimized in terms of HA to β-TCP ratio, pore size, and layers of the SF coating. Mechanical tests showed that the Young's modulus, compressive strength, and toughness of the scaffolds increased from 0.093 ± 0.006 GPa, 2.939 ± 0.54 MPa and 8.531 ± 1.092 MJ m⁻³ to 0.228 ± 0.029 GPa, 52.521 ± 5.29 MPa and 237.757 ± 18.754 MJ m⁻³ (P &lt; 0.001), respectively by coating with SF. To investigate the regenerative potential of the scaffolds, the behavior of cultured mesenchymal stem cells (MSCs) derived from adipose tissue on the samples was evaluated. The results showed that treatment of scaffolds with HPL promoted cell viability and adhesion and alkaline phosphatase (ALP) activity, which makes biphasic calcium phosphate (BCP)/SF/HPL composite scaffolds promising bone substitutes.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00389"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of bioinks for 3D bioprinting of in vitro skeletal muscle constructs","authors":"Rodi Kado Abdalkader ,&nbsp;Kosei Yamauchi ,&nbsp;Satoshi Konishi ,&nbsp;Takuya Fujita","doi":"10.1016/j.bprint.2025.e00396","DOIUrl":"10.1016/j.bprint.2025.e00396","url":null,"abstract":"<div><div>The use of 3D bioprinting to construct <em>in vitro</em> skeletal muscle models presents a promising approach; however, selecting an optimal bioink remains a common challenge. This study focuses on the development and characterization of bioinks for extrusion-based 3D bioprinting, specifically targeting the creation of accurate skeletal muscle models. By exploring various compositions of alginate, gelatin, fibrinogen, and nanofiber cellulose, we evaluate these formulations based on printability and their support for the growth and differentiation of C2C12 myoblast cells.</div><div>While alginate provided a strong, stable matrix for printing scaffolds embedded with C2C12 cells, it did not effectively promote cell growth and differentiation. The addition of fibrinogen to alginate enhanced cell growth and differentiation but was limited mainly to the scaffold surfaces, even with the inclusion of gelatin as a sacrificial ink. Notably, replacing alginate with nanofiber cellulose (NFC) alongside fibrinogen significantly improved cell growth and differentiation, leading to the formation of mature myotubes. Cell distribution was observed both inside and on the surfaces of the scaffolds, indicating effective spatial cell distribution. Furthermore, the scaffolds were tailored to form skeletal muscle bundles anchored between PDMS pillars for contractility testing. Upon exposure to electrical stimulation, the cells displayed measurable displacement, demonstrating contractile function.</div><div>These findings offer valuable insights into optimizing bioink formulations that promote myoblast growth and differentiation into skeletal muscle <em>in vitro</em>, with potential applications in future neuromuscular disease modeling.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00396"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143212070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved alginate bio-ink by recombinant self-assembled cell-sized spider-silk inspired-biopolymer","authors":"Dean Robinson ,&nbsp;Miriam Gubelbank ,&nbsp;Ella Sklan ,&nbsp;Tali Tavor Re'em","doi":"10.1016/j.bprint.2025.e00387","DOIUrl":"10.1016/j.bprint.2025.e00387","url":null,"abstract":"<div><div>Alginate is a natural linear polysaccharide polymer that is extracted from brown seaweed. It is extensively used due to its biocompatibility, ease of handling in aqueous environments, and relatively low cost. Alginate easily forms a hydrogel when crosslinked with a bivalent ion such as calcium. However, alginate hydrogel exhibits low mechanical strength and is cell-inert, having no cell-matrix interactions. To address these limitations and enhance alginate's utility as a bioink for bioprinting, we developed a novel alginate matrix combined with spider- silk, known for its exceptional resilience, elasticity, and strength, as well as its capacity to facilitate cell attachment. The unique recombinant spider-silk biopolymer used in our study (SVX), is synthetically produced, and self-assembles into water-insoluble cell-sized particles that are limited by the cell size in the expression system. These are characterized by a sponge-like structure, and are both biocompatible and non-immunogenic.</div><div>Incorporating synthetic spider-silk into alginate significantly increased the hydrogel's viscosity and compression resilience compared to alginate alone. SVX-enriched alginate exhibited superior printability, characterized by a lower spreading ratio at reduced pressures that is favorable for cell printing. The SVX-enriched alginate also demonstrated higher consistency in spreading ratios across a range of setup conditions. Bioprinting of cells within the SVX-enriched alginate bioink resulted in more homogenous cultures with prolonged and higher cell viability, compared to the larger, more condensed spheroids with lower cell viability observed in alginate bioprinted constructs. These enhanced cell cultures in the SVX-enriched constructs can be attributed to the improved stability of the constructs as well as spider-silk-mediated cell adherence.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00387"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An alginate-cellulose based bioink mimics the viscoelastic features of the melanoma microenvironment and its influence on cell cycle and invasion","authors":"Carolin Eckert ,&nbsp;Sonja Schmidt ,&nbsp;Jessica Faber ,&nbsp;Rainer Detsch ,&nbsp;Martin Vielreicher ,&nbsp;Zan Lamberger ,&nbsp;Philipp Stahlhut ,&nbsp;Evelin Sandor ,&nbsp;Tannaz Karimi ,&nbsp;Rafael Schmid ,&nbsp;Andreas Arkudas ,&nbsp;Oliver Friedrich ,&nbsp;Silvia Budday ,&nbsp;Gregor Lang ,&nbsp;Annika Kengelbach-Weigand ,&nbsp;Anja Bosserhoff","doi":"10.1016/j.bprint.2024.e00384","DOIUrl":"10.1016/j.bprint.2024.e00384","url":null,"abstract":"<div><div>Melanoma, an aggressive tumor from melanocytes, poses challenges despite recent therapeutic advances. Understanding molecular changes in its progression is crucial. Melanoma cells develop in the epidermis, then start spreading into the dermis– the first step of the invasive, progressive process. The dermis is composed of elastic (proteoglycans) and stabilizing (collagens) molecules. To overcome limitations of 2D-cell culture models, we established a 3D-bio-printed dermis model for the analysis of tumor cell features using a blend of alginate and microfibrillar cellulose. Testing different compositions in extrusion-based bioprinting confirmed good printability with high cell viability for AlgCell ink. Mechanical and optical analyses revealed dermis-like viscoelasticity and a pore size allowing nutrition supply and cell movement. We evaluated survival and proliferation of the cells and printed tumor spheroids and determined different migratory behavior comparing alginate to AlgCell. Interestingly, multiphoton microscopy revealed random cellulose fiber distribution around the spheroids after 7 days of cultivation with individual single cells, which had left the tumor spheroid and invaded into the microenvironment. Traditional 2D-models inadequately capture 3D mechanisms like invasion and migration. Our 3D-tumor model mimics the microenvironment, enabling in-depth analyses akin to <em>in vivo</em> conditions. This promises insights into tumor progression and testing of therapeutic interventions.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00384"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinting of natural materials and their AI-Enhanced printability: A review","authors":"Soumaya Grira ,&nbsp;Mohammad Sayem Mozumder ,&nbsp;Abdel-Hamid I. Mourad ,&nbsp;Mohamad Ramadan ,&nbsp;Hadil Abu Khalifeh ,&nbsp;Mohammad Alkhedher","doi":"10.1016/j.bprint.2025.e00385","DOIUrl":"10.1016/j.bprint.2025.e00385","url":null,"abstract":"<div><div>A wide array of biomaterials including proteins, polysaccharides, and other polymers are used to fabricate green 3D printing inks suitable for use in the fields of biotechnology, water treatment, food and agriculture, energy, and bioplastics. Although green materials are sustainable and have many favorable properties, each material has its own limitations. The main focus of this paper is to review the currently available 3D printing biomaterials that can be obtained from natural sources and investigate the artificial intelligence (AI) assisted approaches that can be used to enhance their printability and accelerate their development. Results reveal that above 20 natural materials can be used in 3D printing inks, and their properties vary widely making their development slow and case-dependent. To speed up this process, this study highlights the significance of AI-assisted enhancement techniques and proves that AI can be used in three main aspects of biomaterial development for 3D bioprinting; material property prediction, smart material selection, and printing parameter optimization. It discusses the AI-based systems that have the potential to accelerate the development of 3D printing biomaterials and concludes that these methods are indeed promising but should be further tested, verified, and compared before they are incorporated into the industry. The importance of this study lies in integrating advanced AI systems with sustainable natural materials to leverage their inherent properties and utilize them in various applications for a greener future.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00385"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed cellulose nanofiber-reinforced and iron-crosslinked double network hydrogel composites for tissue engineering applications: Mechanical properties and cellular viability","authors":"Rohit Goyal ,&nbsp;Soumyasri Nikhilesh Mahapatra ,&nbsp;Rashmi Yadav ,&nbsp;Santanu Mitra ,&nbsp;Animesh Samanta ,&nbsp;Anuj Kumar ,&nbsp;Bimlesh Lochab","doi":"10.1016/j.bprint.2025.e00392","DOIUrl":"10.1016/j.bprint.2025.e00392","url":null,"abstract":"<div><div>Additive manufacturing (i.e. 3D printing) is a promising technology for creating three-dimensional (3D) complex tissue-engineered hydrogel structures based on computer digital models resulting from patient-specific anatomical data of the organs. However, besides the printing process, it is worth studying the variation of individual components of the developed hydrogel composites to enable their suitability for tissue engineering. In this work, we shaped 3D printed multi-layered dual (UV- and Fe³⁺ ions)-crosslinked structures using hydrogel-inks composed of polyacrylamide (PAM), alginate (ALG), and cellulose nanofibres (CNFs). For extrusion, ALG in hydrogel precursor ink acted as a viscosity modifier owing to rapid gelation in the presence of low Ca²⁺ ions and CNF provided shear-thinning behavior. With the addition of optimal content of CNF (3 wt%), the mechanical properties of 3D printed composite hydrogel were enhanced and tuned using different fiber orientations. The maximum tensile stress of PAM/ALG_1.5/3CNF composite hydrogel is measured as ∼162 kPa, and maximum tensile toughness as ∼54 kJ/m³ supporting a good fracture resistance. Moreover, CNF-Fe³⁺ loaded 3D printed dual-networked composite hydrogels could disperse energy more efficiently and displayed maximum tensile stress as ∼285 kPa and maximum toughness as ∼200 kJ/m³. Further, In the current study, developed composite structures exhibited enhanced swelling ratio and thermal stability. In addition, finite element (FE) modelling was also exploited to analyze the novel anisotropic composite structures using efficient computational techniques. It is established that varying nanofiber content and fibrils orientation can be utilized to modulate the physicochemical, mechanical, and biological characteristics of printed structures. Overall, PAM/ALG_1.5/3CNF-Fe³⁺ printed composite structures present substantial stretchability, enhanced anisotropic mechanical and physicochemical properties with excellent cytocompatibility.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00392"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations in 3D printing of magnesium alloys and composites for biodegradable biomedical devices","authors":"Aditya Nair ,&nbsp;Shruti Gupta ,&nbsp;Aboli Jangitwar ,&nbsp;Balasubramanian Kandasubramanian","doi":"10.1016/j.bprint.2025.e00390","DOIUrl":"10.1016/j.bprint.2025.e00390","url":null,"abstract":"<div><div>Magnesium is among the plentiful minerals present in natural sources, serving as a crucial macronutrient for the human body, with numerous studies validating its distinctive traits such as remarkable biocompatibility within the human system, diminished stress shielding, and proficient physical and chemical characteristics. These attributes are pivotal elements when employing the mineral in alloys and composites for the fabrication of biomedical components. One particular application involves the utilization of magnesium-based alloys and composites in the creation of coronary stents and bone implants. The ability to manufacture magnesium-based biomedical components with precision and reduced material wastage through additive manufacturing methods has prompted a transition away from the conventional manufacturing processes presently in use. This review aims to offer a thorough assessment of the application of additive manufacturing in producing magnesium alloys and composites for biomedical purposes. The paper comprises a comparative examination of the fabrication methods presently employed for the production of these alloys and composites, with a particular emphasis on various additive manufacturing techniques. Furthermore, it delves into the surface modification of additively manufactured implants, which has shown considerable improvements in biocompatibility and corrosion resistance, which are crucial parameters in the realm of biomedicine.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00390"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomically accurate 3D printed prosthetic incus for ossicular chain reconstruction","authors":"Masoud Mohseni-Dargah ,&nbsp;Christopher Pastras ,&nbsp;Payal Mukherjee ,&nbsp;Kai Cheng ,&nbsp;Khosro Khajeh ,&nbsp;Mohsen Asadnia","doi":"10.1016/j.bprint.2025.e00393","DOIUrl":"10.1016/j.bprint.2025.e00393","url":null,"abstract":"<div><div>Middle ear disease often leads to ossicular erosion, impairing auditory function and frequently requiring ossicular chain reconstruction (OCR) for hearing restoration. Columella-type prostheses, commonly used in OCR, have shown limited success due to issues such as displacement and extrusion, highlighting the need for more effective solutions. This study introduces a 3D-printed prosthesis anatomically resembling the human incus bone, referred to as the titanium prosthetic incus, as a potential device for OCR. Utilising Finite Element Analysis (FEA), CT imaging, and 3D printing, the prosthesis was numerically evaluated, fabricated, and experimentally tested to assess its mechanical performance and anatomical fit. The prosthetic incus demonstrated ossicular vibration comparable to healthy control ears, effectively transmitting sound energy to the inner ear. The results revealed that the prosthetic incus offers superior sound transmission performance, particularly at low frequencies (below 1000 Hz), when compared to the PORP, with similar performance at higher frequencies. Additionally, the prosthetic incus has the potential to improve overall stability over traditional PORP devices, with a reduced risk of displacement due to its precise anatomical fitting. This study also suggests that the approach of contralateral imaging and individualised 3D printing enhances the customisation and accuracy of OCR procedures, potentially reducing operative time and improving long-term outcomes. Furthermore, the cost-effective nature of 3D printing makes this solution both clinically viable and scalable. This innovative technique holds promise for advancing OCR by providing a durable, patient-specific prosthetic option that enhances sound transmission and surgical success rates for patients with middle ear ossicular erosion.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00393"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of oral squamous cell carcinoma microenvironment- A 3D bioprinting approach","authors":"Akhilanand Chaurasia ,&nbsp;Gowri Sivaramakrishnan ,&nbsp;Farah Asa’ad ,&nbsp;Lena Larsson ,&nbsp;Arwa Daghrery ,&nbsp;Joana Marques ,&nbsp;Francesca Spirito ,&nbsp;Vitória Batista Clemente ,&nbsp;Ana Carolina Morais Apolônio ,&nbsp;Mahdieh Alipour ,&nbsp;Rini Tiwari","doi":"10.1016/j.bprint.2024.e00381","DOIUrl":"10.1016/j.bprint.2024.e00381","url":null,"abstract":"<div><h3>Background</h3><div>Oral squamous cell carcinoma (OSCC) presents significant challenges due to its complex microenvironment and invasive characteristics. Traditional two-dimensional (2D) culture systems are inadequate for modelling the intricate features of OSCC, necessitating advanced techniques for better <em>in vitro</em> modelling.</div></div><div><h3>Objective</h3><div>This review aims to explore the applications of 3D bioprinting in modelling the OSCC microenvironment, highlighting the advantages over conventional methods and discussing recent advancements in the field.</div></div><div><h3>Methods</h3><div>The review synthesizes recent literature on 3D bioprinting technologies, focusing on their application in replicating OSCC's microenvironment. Key areas include the integration of various cell types within a biomimetic extracellular matrix, the use of microfluidic systems to study tumor-stromal interactions, and the incorporation of advanced imaging modalities.</div></div><div><h3>Results</h3><div>3D bioprinting allows for the precise fabrication of complex OSCC tumor architectures, incorporating cancer cells, stromal cells, and immune cells. The integration of microfluidic systems facilitates the study of tumor invasion, metastasis, and drug response. Recent advancements in bioink development, particularly the use of patient-derived cells and biomolecules, enhance the physiological relevance of these models. Emerging imaging technologies provide unprecedented insights into the dynamics of OSCC progression within these constructs.</div></div><div><h3>Conclusion</h3><div>3D bioprinting shows immense potential for advancing the understanding of OSCC pathobiology and developing personalized therapeutic strategies. However, challenges such as standardizing bioink formulations and scaling fabrication techniques must be addressed to effectively translate these innovations into clinical practice.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"45 ","pages":"Article e00381"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zirconia-calcium silicate bioactive composites for dental applications using DLP additive manufacturing","authors":"Ahmed Binobaid ,&nbsp;Michele De Lisi ,&nbsp;Josette Camilleri ,&nbsp;Hany Hassanin ,&nbsp;Khamis Essa","doi":"10.1016/j.bprint.2024.e00377","DOIUrl":"10.1016/j.bprint.2024.e00377","url":null,"abstract":"<div><div>Zirconia has outstanding mechanical strength which made it a favourable material dental implants material. However, its use is limited by challenges in bone bonding and elasticity. This paper introduces a novel bioprinting ceramic material by mixing calcium silicate with zirconia to enhance bioactivity. Using the high precision and speed of Digital Light Processing (DLP), this study develops a novel zirconia-calcium silicate slurry for dental applications. The study reports the preparation of zirconia-calcium silicate, formulation of resin compositions, and optimization of the bioprinting, debinding and sintering. Employing a full factorial Design of Experiments (DOE), a systematic approach was implemented to identify optimal printing conditions such as the layer thickness, exposure time, and power. The results show that slurries formulated with BYK-111 as the dispersant and ACMO/PEGDA/TPO resin, coupled with 80 wt% solid loading, achieved the most favourable rheological properties, cure depth, and printing accuracy. The optimal printing conditions were 0.75 s exposure time, 300 % exposure power, and 30 μm layer thickness, ensured a relative density of the sintered implants exceeding 95 %. This study advances dental implant materials by introducing a novel DLP biomaterial with a slurry formulation, presenting significant implications for clinical applications and future research in developing advanced dental and medical implants.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"45 ","pages":"Article e00377"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143101937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}