Bioprinting最新文献

{"title":"Reproducing viscoelastic properties of soft tissues in 3D printed silicone models by two-phase infill tuning","authors":"Stephan Dehen ,&nbsp;Felix Groß ,&nbsp;Andrea Lorenz ,&nbsp;Dieter H. Pahr ,&nbsp;Andreas G. Reisinger","doi":"10.1016/j.bprint.2025.e00408","DOIUrl":"10.1016/j.bprint.2025.e00408","url":null,"abstract":"<div><div>Anatomical models are essential tools for teaching, patient education, or training. Recent developments in 3D printing enabled the production of customised models based on individual imaging data. Although most 3D printing processes can accurately reproduce anatomical structures geometrically, they lack similarity in haptic properties. Therefore, in this study, we investigated the influence of highly viscous silicone oil injections in 3D printed silicone samples on enhancing viscoelastic behaviour. For this, 72 specimens with 3 different infill densities (20 %, 30 %, 40 %) were printed and tested using stress relaxation tests. Afterwards, they were filled using 3 different high viscous silicone oils (1 kPa<span><math><mi>⋅</mi></math></span>s, 5 kPa<span><math><mi>⋅</mi></math></span>s, 10 kPa<span><math><mi>⋅</mi></math></span>s) and retested. The material properties of the silicone infill/silicone oil combination were extracted from the structural properties of the tested samples using an optimisation strategy based on a finite element model to get the material response for the infill only. Alongside the infill density, the storage modulus increases from 28.0 to 52.3 kPa for empty samples. By adding high viscous silicone oil the loss modulus is increased from 3.3–5.6 kPa up to 12.0–20.0 kPa. The resulting loss tangent increases from 0.10–0.12 to 0.28–0.29 for the different infill densities. With this range of possible viscoelastic properties, several different biological soft tissues can be modelled. It could be proven that a silicone oil injection is a promising way to increase the loss moduli of 3D printed silicone samples, greatly increasing the design space of possible printable viscoelastic properties.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"48 ","pages":"Article e00408"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143828836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topology optimization and manufacturing of maxillofacial patient specific implant using FEA and AM","authors":"Rakesh Koppunur ,&nbsp;K. Ramakrishna ,&nbsp;A. Manmadhachary ,&nbsp;Dama Kiran Kumar ,&nbsp;V. Sridhar","doi":"10.1016/j.bprint.2025.e00412","DOIUrl":"10.1016/j.bprint.2025.e00412","url":null,"abstract":"<div><div>Patient-specific implants have gained significant attention due to their adaptability and precision in addressing individual anatomical variations. However, optimizing the strength-to-weight ratio remains a critical design challenge. This study focuses on the analysis and topological optimization of patient-specific implants to enhance their mechanical performance while minimizing weight. Finite Element Analysis (FEA) is employed to evaluate the maximum mastication force that a maxillofacial implant can withstand, ensuring that stress distribution and deformation remain within acceptable limits. Given the crucial role of mastication forces in implant stability and longevity, design iterations are conducted to achieve an improved strength-to-weight ratio. The optimized design undergoes validation through FEA under identical boundary and loading conditions. Results indicate a 4.43 % reduction in implant weight with a marginal 4 μm increase in deformation compared to the non-optimized design. To manufacture the optimized implant with high precision and structural integrity, Direct Metal Laser Sintering (DMLS), an advanced Additive Manufacturing (AM) technique, is utilized. This approach enables the fabrication of complex geometries while maintaining superior mechanical properties, ensuring the feasibility of the optimized implant for clinical applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"48 ","pages":"Article e00412"},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed PLA/Fe3O4/MgO hybrid composite scaffolds with improved properties","authors":"Reyhaneh Ramezani ,&nbsp;Reza Alizadeh ,&nbsp;Sheyda Labbaf","doi":"10.1016/j.bprint.2025.e00398","DOIUrl":"10.1016/j.bprint.2025.e00398","url":null,"abstract":"<div><div>Fused deposition modeling was successfully used to print porous scaffolds, using filaments of pure PLA, PLA/15 wt% Fe₃O₄ and PLA/15 wt% Fe₃O₄/5 wt% MgO. The magnetic, mechanical, thermal, and cellular properties of these samples were systematically evaluated and compared. The findings reveal that incorporating Fe₃O₄ enhances the magnetization saturation of PLA without compromising its mechanical and thermal integrity. Moreover, weight loss tests in phosphate-buffered saline solution indicated that the PLA/Fe₃O₄/MgO composite showed the highest degradation rate after 65 days. Biological assays confirmed enhanced cell adhesion and viability for the PLA/Fe₃O₄ and PLA/Fe₃O₄/MgO composites compared to pure PLA. These results demonstrate that the PLA/Fe₃O₄ and PLA/Fe₃O₄/MgO composites are promising alternatives of pure PLA for biomedical applications, addressing its inherent limitations, especially in cases where detection of implant by <em>X</em>-ray is required after implantation.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"47 ","pages":"Article e00398"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FK506 binding protein like, FKBPL, as a novel therapeutic target in 2D and 3D bioprinted, models of cardiac fibrosis","authors":"Michael Chhor ,&nbsp;Shreya Barman ,&nbsp;Fatemeh Heidari ,&nbsp;Amy L. Bottomley ,&nbsp;Tracy Robson ,&nbsp;Kristine McGrath ,&nbsp;Lana McClements","doi":"10.1016/j.bprint.2025.e00397","DOIUrl":"10.1016/j.bprint.2025.e00397","url":null,"abstract":"<div><h3>Background</h3><div>Cardiac fibrosis characterised by increased collagen deposition and extracellular matrix (ECM) remodeling is one of the main causes of heart failure. Inflammation and hypoxia are key processes leading to cardiac fibrosis although the mechanisms are poorly understood. In this study, we developed an innovative 3D bioprinted model of cardiac fibrosis using tunable matrices. The role of an anti-angiogenic protein, FK506 binding protein like (FKBPL) was then elucidated, for the first time, using both 2D and 3D bioprinted, models of cardiac fibrosis.</div></div><div><h3>Methods</h3><div>3D bioprinted model of cardiac fibrosis was developed using fetal fibroblast cells (HFF08), customised ECM cardiac components and pro-fibrotic/hypoxic factors (TGF-β, 10 ng/ml, DMOG, 1 mM) ± FKBPL mimetic (AD-01, 100 mM). In parallel, 2D <em>in vitro</em> models were also employed.</div></div><div><h3>Results</h3><div>In the 3D bioprinted model, fibroblasts formed networks spontaneously, which were stimulated by all treatments (p &lt; 0.05–0.0001). This was in conjunction with a trend towards reduced FKBPL expression, particularly in the presence of DMOG/AD-01 treatment. In 2D cell culture, AD-01 potentiated TGF-β-induced <em>col1a1</em> (p &lt; 0.0001) and <em>mmp2</em> mRNA (p &lt; 0.05) expression whereas DMOG or reduced FKBPL expression with AD-01 abrogated this (p &lt; 0.05–0.001). Following siRNA FKBPL transfection, α-SMA was reduced (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>This 3D bioprinted model of cardiac fibrosis in conjunction with 2D cell models could be used for biomarker and drug therapy screening towards accelerating the development of treatments for this hard-to-treat condition. Low FKBPL expression could be protective in cardiac fibrosis through the reduction in collagen production and α-SMA expression, or TGF-β/HIF-1α-mediated effects. Therapeutic strategies that inhibit FKBPL should be explored to abrogate cardiac fibrosis.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"47 ","pages":"Article e00397"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143428692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of 3D printed Voronoi microarchitecture bone scaffold using Taguchi-grey relational analysis","authors":"Rochmad Winarso ,&nbsp;Sugeng Slamet ,&nbsp;Rianto Wibowo ,&nbsp;Sigit Arrohman ,&nbsp;Akhmad Zidni Hudaya ,&nbsp;Rifky Ismail ,&nbsp;Jamari ,&nbsp;Athanasius Priharyoto Bayuseno","doi":"10.1016/j.bprint.2025.e00402","DOIUrl":"10.1016/j.bprint.2025.e00402","url":null,"abstract":"<div><div>3D printing bone scaffolds is a cutting-edge approach in bone tissue engineering, potentially resolving critical-sized bone defect challenges. While current research primarily focuses on tensile parameters in printing, compressive parameters are often overlooked despite their crucial role in scaffold performance. This study aimed to optimize the mechanical properties of bone scaffolds featuring Voronoi microarchitecture through tailored printing parameters. Utilizing the Taguchi method and Grey Relational Analysis (GRA), significant variations in mechanical parameters such as elastic modulus and compressive strength were identified among specimen groups. Key printing factors including layer height, line width, printing temperature, and printing speed proved pivotal in influencing the compressive strength and elastic modulus of polylactic acid (PLA) used in 3D printing. This research demonstrates the novelty of combining the Taguchi-GRA approach with the Voronoi microarchitecture to achieve superior mechanical properties. Specifically, optimal settings layer height of 0.0625 mm, line width of 0.25 mm, printing temperature of 215 °C, and printing speed of 55 mm/s, yielded scaffolds with enhanced compressive strength and elastic modulus, meeting biomechanical requirements for bone regeneration. Further investigation is warranted to establish comprehensive guidelines for achieving consistent mechanical excellence in 3D-printed PLA components, thereby advancing the efficacy and reliability of bone scaffold applications. The findings of this study provide a foundation for standardizing 3D printing protocols for bone scaffolds, bridging the gap between experimental designs and clinical applications. By addressing critical bottlenecks and introducing innovative solutions, this research contributes to advancing the field of bone tissue engineering and improving outcomes in regenerative medicine. patient outcomes in bone tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"47 ","pages":"Article e00402"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143601725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of 3D soft polymeric constructs at high structural integrity through bioprinting optimization of suspended hydrogels","authors":"Miriam Seiti ,&nbsp;Elena Laura Mazzoldi ,&nbsp;Stefano Pandini ,&nbsp;Eleonora Ferraris ,&nbsp;Paola Serena Ginestra","doi":"10.1016/j.bprint.2025.e00403","DOIUrl":"10.1016/j.bprint.2025.e00403","url":null,"abstract":"<div><div><em>In vitro</em> models of soft tissues, such as neural, vitreous, or hematopoietic human tissues, require three-dimensional (3D), soft, and functionalized constructs that mimic the complex extracellular microenvironment and support tissue growth and differentiation. While bioprinting has gained significant interest in bioengineering, there is limited research on process control for the biomanufacturing of soft tissues, which is still in its early stages. Material extrusion of suspended hydrogels has shown promise in processing low-viscosity inks, but challenges in developing bioinks that maintain good shape fidelity, repeatability, and long-term stability in culture media have slowly progressed. In this study, we optimize the bioprinting process for the suspended extrusion of low-viscosity autoclaved bioinks (<em>η</em> = 121 ± 4 mPa s from 50 to 100 s⁻¹) into 3D soft polymeric constructs. The stability of the process is evaluated using a full factorial design approach. Bone marrow-derived stromal cells are bioprinted at a low concentration (5 × 10⁵ cells/mL), showing excellent metabolic activity up to day 7 compared to 2D cell culture controls. The final soft constructs exhibit a compression Young's Modulus of 7.8 ± 0.9 kPa, a water uptake of 60 %, and minimal gel degradation over 21 days. This work offers new insights into optimizing this advanced bioprinting process towards the development and study of 3D <em>in vitro</em> soft tissue models.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"47 ","pages":"Article e00403"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural, mechanical and biomedical properties of 3D-printed Cu-doped Fe3O4/58S bioactive glass/polycaprolactone composite scaffold for bone tissue regeneration","authors":"Mojtaba Rajabinezhad ,&nbsp;Mohammad Saeid Abbasi ,&nbsp;Farnaz Heidari Laybidi ,&nbsp;Mohammadjavad SharifianJazi ,&nbsp;Mohammad Khodaei ,&nbsp;Abbas Bahrami","doi":"10.1016/j.bprint.2025.e00400","DOIUrl":"10.1016/j.bprint.2025.e00400","url":null,"abstract":"<div><div>This research investigates the mechanical, structural and biomedical implications of adding copper-doped magnetite nanoparticles, composited with 58S bioactive glass, to the 3D-printed polycaprolactone (PCL) scaffold. Cu-doped magnetite nanoparticles can be potentially used in hyperthermia and anti-bacterial applications. Additionally, the addition of bioactive glass was intended to promote bone tissue regeneration, hence creating a multi-purpose 3D-printed PCL scaffold. The PCL-nanocomposite mixtures were 3D printed using FDM method. Cu-doped magnetite nanoparticles-58S bioactive glass composite powders and 3D printed scaffolds were characterized using different techniques, including X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), Fourier-transform infrared spectroscopy (FTIR), and vibrating sample magnetometer (VSM). Cell viability, bioactivity, and anti-bacterial properties of scaffolds were also investigated. XRD/FESEM/FTIR results confirmed successful synthesis of Cu-doped magnetite nanoparticles-58S bioactive glass composite powder mixture with a perfect superparamagnetic behavior. Results also showed that the addition of secondary particles to the PCL is associated with some noticeable impacts on the wettability, roughness, and mechanical properties of printed scaffolds, with the best properties attained in the sample with 20 % of added secondary particles. Assessments of biomedical properties of printed specimens showed that the optimum printed scaffold has great anti-bacterial performance and promising cell viability and bioactivity, making is a great candidate for bone tissue engineering applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"47 ","pages":"Article e00400"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-material 3D bioprinting of human stem cells to engineer complex human corneal structures with stroma and epithelium","authors":"P. Puistola ,&nbsp;S. Huhtanen ,&nbsp;K. Hopia ,&nbsp;S. Miettinen ,&nbsp;A. Mörö ,&nbsp;H. Skottman","doi":"10.1016/j.bprint.2025.e00391","DOIUrl":"10.1016/j.bprint.2025.e00391","url":null,"abstract":"<div><div>Developing cost-effective and scalable multi-material bioprinting technologies that combine multiple cell types is crucial to produce biomimetic, complex human tissue substitutes and overcome the scarcity of transplantable tissues. These technological developments can revolutionize the treatment of several conditions currently dependent on donor tissues, such as corneal blindness. Here, corneal structures consisting of two layers, stroma and epithelium, were manufactured by extrusion-based 3D bioprinting. To take steps towards clinical translation of bioprinting, three clinically compatible hyaluronic acid based bioinks were combined with human adipose tissue and induced pluripotent stem cell derived cell types. Each of the three bioinks was customized to suit the needs of different cells and to provide mechanical stability for the bioprinted structure. Along with offering a 3D environment with excellent cytocompatibility, these bioprinted corneal structures facilitated cellular interactions and network formation, which are essential for creating functional tissue substitutes. Consequently, this study provides important insight on how to bring together the technical aspects of multi-material bioprinting as well as the biological relevance and scalability of the bioprinted constructs, advancing the field of additive manufacturing for clinical applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00391"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of organ-on-a-chip using microfluidics","authors":"S. Ying-Jin ,&nbsp;I. Yuste ,&nbsp;E. González-Burgos ,&nbsp;D.R. Serrano","doi":"10.1016/j.bprint.2025.e00394","DOIUrl":"10.1016/j.bprint.2025.e00394","url":null,"abstract":"<div><div>The use of microfluidic devices represents a significant advancement beyond conventional techniques in the development of innovative <em>in vitro</em> assays. Microfluidic chips are specialized devices that precisely control fluids at the microscale level through intricate microchannels, enabling the replication of physical and chemical conditions. When combined with tissue engineering, these chips have evolved into highly specialized tools known as Organ-on-a-Chip (OoC) devices, which can simulate the physiology and functionality of various human tissues and organs. OoC devices are cutting-edge technologies that integrate a biological component representing the target organ with a microfluidic component that mimics blood flow. This combination allows for the replication of biological structures with a more accurate representation of the <em>in vivo</em> physiological cellular microenvironment, which can be finely tuned by adjusting the flow rate and composition. As a result, novel microfluidic models for <em>in vitro</em> research can overcome the limitations of traditional 2D and 3D static cell cultures, enabling faster clinical translation and more precise predictions of the efficacy, safety, pharmacodynamics, and pharmacokinetics of new drugs. This review will discuss various techniques for fabricating OoCs and their applications in mimicking different physiological microenvironments.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00394"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of medical 3D printing, printable biomaterials, prosthetic and regenerative dental applications","authors":"Mohammed Ahmed Alghauli ,&nbsp;Rola Aljohani ,&nbsp;Waad Aljohani ,&nbsp;Shahad Almutairi ,&nbsp;Ahmed Yaseen Alqutaibi","doi":"10.1016/j.bprint.2025.e00395","DOIUrl":"10.1016/j.bprint.2025.e00395","url":null,"abstract":"<div><div>This review explores the rapid advancements in additive manufacturing, particularly 3D printing, within dentistry, focusing on bioprinting. It highlights the technology's efficiency, cost-effectiveness, and environmental sustainability while comprehensively analyzing its historical development, classification, and applications. The study compares additive manufacturing with conventional subtractive methods like CNC milling and evaluates the materials used. A thorough literature search across PubMed, Scopus, Web of Science, Cochrane, and Google Scholar was conducted, focusing on recent developments in 3D printing and CAD/CAM technologies in dentistry. The review identifies key applications, including surgical guides and root analog implants in implant dentistry, as well as the production of dental models, denture bases, and metal frameworks. Though prosthodontics is in the early stages of adopting 3D printing, advancements in materials and technologies are paving the way for its broader application. This review provides valuable insights for researchers and developers, emphasizing the potential of additive manufacturing to become a dominant chairside production method.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"46 ","pages":"Article e00395"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143093934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}