Bioprinting最新文献

{"title":"Current landscape and opportunities in the development of bioengineered in-vitro vascularized liver tissue models","authors":"Kshama Kumari ,&nbsp;Arka Sanyal ,&nbsp;Preeti Rawat ,&nbsp;Vinit Kumar ,&nbsp;Manoj Garg ,&nbsp;Debrupa Lahiri ,&nbsp;Sourabh Ghosh ,&nbsp;Prakash Baligar","doi":"10.1016/j.bprint.2024.e00350","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00350","url":null,"abstract":"<div><p>The complications in liver functioning arising due to hepatic disorders are a major contributor of mortality worldwide, with transplantation being the only resort for patients with severe cases. Due to liver's direct role in drug metabolism, fabrication on functional liver tissue models is eventually becoming a necessity for high-throughput drug screening applications. Tissue engineering approaches could provide an answer to the drooping supply by allowing for the fabrication and printing of a fully operational, implantable, and sustainable liver tissues. Moreover, such bioengineered tissues can be made to resemble their native counterparts. 3D bioengineering strategies including 3D bioprinting and microfluidic-based liver-on-chip models stand out in this regard due to their potential to create physiologically relevant microenvironment/niches for the biofabricated tissues. Nonetheless, achieving vascularization in such bioengineered tissues is still considered one of the biggest bottlenecks for engineers. The incorporation of blood vessels made from endothelial cells (ECs) is addressed in vasculogenesis while angiogenesis investigates generating new vessels from preexisting vasculature. Overall, vascularization is essential for the survival, function, and integration of bioprinted liver tissues, making it a key focus area in the development of functional liver substitutes for regenerative medicine and drug testing applications. This review paper focuses on the opportunities and difficulties of performing vascularization and angiogenesis in 3D bioengineered-based liver tissue models. Particularly, this paper delves into aspects such as methods of bioengineering, bioinks used, analysis techniques, advantages, limitations, and prospects related to 3D bioengineered liver tissue models as well as vascular engineering in general.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"41 ","pages":"Article e00350"},"PeriodicalIF":0.0,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141482873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diffusion coefficients in scaffolds made with temperature controlled cryoprinting and an ink made of sodium alginate and agar","authors":"Leo Lou ,&nbsp;Boris Rubinsky","doi":"10.1016/j.bprint.2024.e00348","DOIUrl":"10.1016/j.bprint.2024.e00348","url":null,"abstract":"<div><p>Temperature Controlled Cryoprinting (TCC), is a tissue engineering technique wherein each deposited voxel is frozen with precise control over cooling rates and the direction of freezing. This control allows for the generation of ice crystals with controlled shape and orientation. Recently we found that the macroscale fidelity of the TCC print is substantially improved by using a 3D printing ink composed of a mixture of two compounds: one that solidifies through chemical crosslinking (sodium alginate) and another that solidifies through physical (thermal) effects (agar). In this study we examine the hypothesis that the combination of sodium alginate and agar, affects also the fidelity of the microstructure and thereby the diffusivity of the scaffold. The ability of this technology to generate controlled diffusivity within the tissue scaffold was examined with a directional solidified TCC sample using fluorescence recovery after photobleaching (FRAP) and scanning electron microscope (SEM). We find that the diffusion coefficient in m²/s × 10⁻¹⁰ is: 1.62 <span><math><mrow><mo>±</mo></mrow></math></span> 1.27 for the unfrozen sample, 2.40 <span><math><mrow><mo>±</mo><mspace></mspace><mn>1</mn></mrow></math></span>.54 for the rapidly frozen sample and <span><math><mrow><mn>9.72</mn><mo>±</mo></mrow></math></span> 4.50 for the slow frozen sample. This points to two conclusions. One is that the diffusivity is slow frozen samples is higher than that in unfrozen samples and in rapidly frozen sample. A second observation is that a relatively narrow range of diffusivity variance was obtained when using 2%w/v sodium alginate and 2%w/v of agar. However, when the concentration of agar was reduced to 0.5w/v a much wider spread of diffusivities was measure, <span><math><mrow><mn>4.07</mn><mo>±</mo><mn>1</mn></mrow></math></span>.65. This suggests that the addition of agar has also an effect on the microscale fidelity, and consequently the diffusivity. The anisotropic diffusion properties of TCC-printed directional solidification samples were also validated through both FRAP and SEM.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"41 ","pages":"Article e00348"},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141403908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additive manufacturing of a low modulus biomedical Ti–Nb–Ta–Zr alloy by directed energy deposition","authors":"Saurabh Kumar Gupta ,&nbsp;Sriram Bharath Gugulothu ,&nbsp;Eugene Ivanov ,&nbsp;Satyam Suwas ,&nbsp;Kaushik Chatterjee","doi":"10.1016/j.bprint.2024.e00349","DOIUrl":"10.1016/j.bprint.2024.e00349","url":null,"abstract":"<div><p>While β titanium alloys have garnered extensive attention as a new generation of biomedical materials designed to mitigate stress shielding due to their low modulus, the realm of additive manufacturing for these alloys is still in its nascent stages. This study focuses on the additive manufacturing of Ti–35Nb–5Ta–7Zr alloy powder via directed energy deposition (DED). The primary objectives were assessing the feasibility of employing DED for this alloy powder and identifying processing parameters to achieve nearly dense components. Systematic exploration of the effect of various processing parameters was performed, and the resultant impact on the densification of the produced specimens was studied. Comprehensive analysis of the microstructure, mechanical properties, electrochemical behavior, and cell studies of fully dense sample coupons were performed. These fully dense samples were found to exclusively comprise the β phase of titanium, resulting in a reduced modulus of elasticity (approximately 44–47 GPa) resulting in high yield strength to elastic modulus ratio. Microstructural examinations revealed the presence of both columnar and equiaxed dendrites, with grains transitioning from columnar to equiaxed (known as CET). Electrochemical testing of the coupons indicated exceptional corrosion resistance in the additively manufactured TNZT alloy. Pre-osteoblasts cultured on the alloys showed good attachment, viability, and growth to confirm cytocompatibility. These findings unveiled the attainment of high strength, favorable ductility, a low elastic modulus, excellent corrosion resistance, and cytocompatibility in dense samples created via DED of Ti–35Nb–5Ta–7Zr. These outcomes hold immense significance for the production of patient-specific medical implants manufactured from β-Ti alloys.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"41 ","pages":"Article e00349"},"PeriodicalIF":0.0,"publicationDate":"2024-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141404510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility of 3D printed plastics for use in bioreactors","authors":"Joseph P. Licata,&nbsp;Helena Slupianek,&nbsp;Shahrizoda Rizokulova,&nbsp;Jonathan A. Gerstenhaber,&nbsp;Peter I. Lelkes","doi":"10.1016/j.bprint.2024.e00347","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00347","url":null,"abstract":"<div><p>Three-dimensional (3D) printing has the potential to be used for rapid-prototyping and inexpensive fabrication of bioreactors for advanced cell and tissue culture. However, the suitability of materials used for 3D printing these bioreactors that will be in direct contact with cells and culture media remains to be established. Many of the most common low-cost materials have not been thoroughly tested under stringent cell culture conditions, especially not with highly sensitive human cell types, such as induced pluripotent stem cells (hiPSCs). This study aims to characterize some 3D printed plastics, such as thermoplastics and photopolymers, focusing on the toxicity/cytocompatibility of the materials as assessed by hiPSC viability, retention of pluripotency, and cardiogenic differentiation potential. Experiments were conducted in a manner that simulates contact between 3D printed plastics and cell culture media, as found in a 3D printed bioreactor. Both photopolymers tested here reduced the viability of hiPSCs, but not of primary human fibroblasts, highlighting the importance of carrying out these tests with the cells of interest. The thermoplastics did not adversely affect stem cell viability, pluripotency, or cardiac differentiation potential. However, except for Nylon12, all thermoplastics deformed during autoclaving, leading us to choose Nylon12 as the most suitable material for bioreactor fabrication. This study represents a step forward in the use of 3D printing for the rapid, low-cost fabrication of custom-designed bioreactors.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"40 ","pages":"Article e00347"},"PeriodicalIF":0.0,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141302514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printing adoption in NHS trusts within the United Kingdom","authors":"Rafay Ul Azeem ,&nbsp;Shokraneh K. Moghaddam ,&nbsp;Richard Kaye ,&nbsp;Malcolm MacKenzie ,&nbsp;Vincenzo Di Ilio ,&nbsp;Yusuf Umar ,&nbsp;Yuen-Ki Cheong","doi":"10.1016/j.bprint.2024.e00346","DOIUrl":"10.1016/j.bprint.2024.e00346","url":null,"abstract":"<div><p>Additive manufacturing and 3D printing is being widely adopted by the medical industry. This study provides a comprehensive overview of the current state of 3D printing technology in NHS trusts across the UK. Data was collected through a survey using the freedom of information act. The survey revealed that 53 NHS trusts (∼25 %) across the UK are utilising the technology, with a diverse range of strategies and applications. The most common application was the creation of guides and models, used for pre-operative planning, intraoperative guidance, and educational purposes. The study also highlights the regulatory and ethical considerations involved in 3D printing in healthcare. The findings indicate that there are no 3D printing specific standards or guidelines being followed for medical devices and therefore underscores the need for clear and consistent regulatory guidelines to be established. As the 3D printing technology continues to advance, its applications in healthcare are expected to expand rapidly, warranting further research into its impact on patient outcomes and healthcare costs.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"41 ","pages":"Article e00346"},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141279412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymeric nanomaterials in 3D bioprinting for tissue engineering and drug delivery applications","authors":"Sarang Han ,&nbsp;John P. Fisher ,&nbsp;Antonios G. Mikos ,&nbsp;Katie J. Hogan","doi":"10.1016/j.bprint.2024.e00345","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00345","url":null,"abstract":"<div><p>Nanoparticles have been broadly investigated in 3D bioprinting (3DBP) for various purposes, including drug delivery, enhanced mechanical performance, biocompatibility, and bioactivity.</p><p>While polymeric nanoparticles have been widely studied for functionalization and drug delivery purposes, current reviews lack investigation of their application for 3DBP, where polymeric nanoparticles can also add unique properties in composition and application for 3DBP.</p><p>Both natural and synthetic polymeric nanoparticles have been employed in 3DBP, with natural polymers providing a strong advantage for biocompatibility and bioactivity and synthetic polymers enabling more control over nanomaterial properties. In 3D printed structures, the colloidal network between polymeric nanoparticles can enhance rheological and mechanical properties and printability. Additionally, these nanomaterials may introduce stimuli responsive elements and deliver key biomolecules, including growth factors or medications. This paper discusses the current application of polymeric nanoparticles and highlights their potential in 3DBP for tissue engineering and drug delivery specifically.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"40 ","pages":"Article e00345"},"PeriodicalIF":0.0,"publicationDate":"2024-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Compression cycling of 3D-printed meniscal tissues in vitro using a custom bioreactor","authors":"Joseph R. Loverde ,&nbsp;Maria E. Piroli ,&nbsp;Kristin H. Gilchrist ,&nbsp;Jason Barnhill ,&nbsp;J. Kenneth Wickiser ,&nbsp;Vincent B. Ho ,&nbsp;George J. Klarmann","doi":"10.1016/j.bprint.2024.e00344","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00344","url":null,"abstract":"<div><p>An estimated 750,000 arthroscopic knee operations are performed in the United States each year, and many are due to a torn meniscus. Transplantation with donor tissue is the gold standard of care in cases where the meniscus cannot be repaired. However, there is a limited supply of transplantable tissue, which may not be the ideal size or shape for the recipient. 3D printing and tissue engineering have been used to produce replacement tissue of specified shape and size, but none offer the compressive modulus or durability of adult-derived tissue. While biomechanical loading of engineered tissues is known to increase mechanical strength, no current paradigms provide sufficient strength. Instead, a combinatorial approach addressing both physiological form and function has emerged as a promising strategy. In this work, anisotropic menisci were bioprinted using ink composed of collagen types I &amp; II, chondroitin sulfate, and mesenchymal stem cells. After printing, a custom bioreactor was used to apply cyclic compression within an incubator throughout the culture period. Compression cycled prints containing cells maintained viability for 3 weeks, while the mechanical strength of cellularized prints increased after 1 week. However, print dimensions and mass of cellular prints decreased over time independent of compression, while glycosaminoglycans were lost from the prints into the culture media. The expression of eight genes were significantly altered due to compression cycling. This work demonstrated that bioprinted menisci containing live cells can be successfully compressed over long time periods in culture without cell death, and despite changing print dimensions, cells under compression contributed to meniscal strengthening whereas acellular prints consistently weaken. By optimizing structure, culture conditions, and compression paradigms, the strength of bioprinted menisci may approach that of native tissue, and this combinatorial approach may reduce or eliminate the need for cadaveric tissues for allograft transplants.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"40 ","pages":"Article e00344"},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140824360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Top 10 directions in lithography 3D printing","authors":"Ruslan Melentiev ,&nbsp;Maryna Melentieva ,&nbsp;Nan Yu","doi":"10.1016/j.bprint.2024.e00343","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00343","url":null,"abstract":"<div><p>Lithography 3D printing technologies such as stereolithography (SLA), two-photon polymerization (TPP), digital light processing (DLP), and other approaches based on vat photopolymerization effect, have been continuously dominating the 3D printing market creating tremendous impact on global economy and society over the past 30 years. The vibrant question is where lithography 3D printing research is heading now? In this study, we conduct a bibliometric analysis and literature review to identify the top 10 research directions that will drive the development of lithography 3D printing in the following decade. We analyzed metadata of nearly ten thousands articles to reveal the evolution of the hottest keywords, most appreciated articles, and other factors in field of lithography 3D printing over the past 30 years. Based on the mined data and literature review, we envision and discus 10 directions that are either emerging or shall emerge promptly, namely tissue engineering (1), DLP of ceramics (2) and metals (3), volumetric printing (4), microneedles printing (5) 4D printing and smart materials (6), metamaterials (7), hot lithography (8), diamond printing (9), and multimaterial printing (10). Recent advances and challenges of each direction were outlined delivering focal points for further research.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"40 ","pages":"Article e00343"},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140807077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent frontiers in biofabrication for respiratory tissue engineering","authors":"Amanda Zimmerling ,&nbsp;Nuraina Anisa Dahlan ,&nbsp;Yan Zhou ,&nbsp;Xiongbiao Chen","doi":"10.1016/j.bprint.2024.e00342","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00342","url":null,"abstract":"<div><p>Respiratory tissue engineering offers a robust framework for studying cell-cell and host-pathogen interactions in a tissue-like environment and offers a platform for studying lung tissue regeneration and disease mechanisms. However, the challenge of replicating dynamic three-dimensional (3D) microenvironments is a huge obstacle with existing technology. Current animal models and two-dimensional cell culture models do not replicate <em>in vivo</em> conditions seen in human lungs, thus research utilizing these techniques often fails to help alleviate the global burden of respiratory diseases. Respiratory tissue engineering has been drawing significant attention over the past decade. Particularly with the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many inspiring developments and advances have been reported. This review presents the recent advances of respiratory tissue engineering focusing on 3D bioprinting, organ-on-a-chip, and organoid technologies. It also provides an overview of recent attempts to integrate biomechanical stimulus with the aim of improving the integrity of 3D constructs and enhancing cellular propagation. This review addresses the challenges inherent in existing 3D respiratory models and discusses the future prospects of research in this field, urging continuing innovation and investment toward the success of respiratory tissue engineering and increasing clinical relevance.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"40 ","pages":"Article e00342"},"PeriodicalIF":0.0,"publicationDate":"2024-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405886624000149/pdfft?md5=22791a8ae640933af1f90e8352d6d332&pid=1-s2.0-S2405886624000149-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140633432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and characterization of 3D printed pore gradient hydrogel scaffold for bone tissue engineering","authors":"Fariza Mukasheva ,&nbsp;Muhammad Moazzam ,&nbsp;Bota Yernaimanova ,&nbsp;Ahmer Shehzad ,&nbsp;Ainur Zhanbassynova ,&nbsp;Dmitriy Berillo ,&nbsp;Dana Akilbekova","doi":"10.1016/j.bprint.2024.e00341","DOIUrl":"https://doi.org/10.1016/j.bprint.2024.e00341","url":null,"abstract":"<div><p>Macroporous hydrogel scaffolds are widely used in tissue engineering to promote cell growth and proliferation. Aiming to enhance cell seeding efficiency and facilitate the osteodifferentiation of mesenchymal stem cells, this study demonstrates the fabrication of pore gradient biodegradable hydrogel scaffolds inspired by natural bone structure for bone tissue engineering applications. The scaffolds were fabricated via extrusion-based 3D printing, using sequential deposition of three customized Gelatin/Oxidized Alginate - based inks with subsequent cryogenic crosslinking for permanent structure fixation. The resulting constructs were characterized and featured a continuous gradient morphology with pore sizes ranging from 10 to 300 μm. The gradient scaffolds exhibited improved mechanical stability, with a compression resistance of 149 kPa, as opposed to the non-gradient scaffold's 116 kPa at 70 % strain, and a sustained degradation rate with only a 10 % loss of its initial weight within three weeks. Gradient scaffolds demonstrated a doubling of cell seeding efficiency to 47 % with dense and homogeneously distributed cellular layers, as evidenced by confocal and electron microscopy. Furthermore, the gradient scaffolds demonstrated superior osteodifferentiation, with significantly higher ALP and DMP1 production and enhanced extracellular matrix mineralization compared to gradientless macroporous scaffolds. This study provides insights into the design of macroporous scaffolds and emphasizes the advantages of pore gradient over homogeneous gradientless morphologies.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"39 ","pages":"Article e00341"},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140558042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}