Bioprinting最新文献

{"title":"A comprehensive review on bioink based microfluidic devices","authors":"Kajal P. Chamate ,&nbsp;Bhuvaneshwari D. Patil ,&nbsp;Nikita V. Bhosale ,&nbsp;Nutan V. Desai ,&nbsp;Prasad V. Kadam ,&nbsp;Avirup Chakraborty ,&nbsp;Ravindra V. Badhe","doi":"10.1016/j.bprint.2024.e00371","DOIUrl":"10.1016/j.bprint.2024.e00371","url":null,"abstract":"<div><div>Microfluidics represents a methodology facilitating the manipulation of minute fluid volumes via microchannels, with wide-ranging applications across biomedical and pharmaceutical research, environmental monitoring, and clinical diagnostics. This discourse delves into the materials utilized in microfluidic devices, their fabrication techniques, and their diverse applications, with a specific focus on variants constructed from glass, paper, metal, and polymers. Additionally, it explores bioprinting methodologies aimed at generating three-dimensional (3D) tissue structures employing bioink for microfluidic system. Bioprinting nurtures the development of functional tissue models essential for tissue engineering, drug screening initiatives, and the evolution of organ-on-a-chip technologies. The discussion extends to an examination of the merits and demerits of various bioinks, such as gelatine methacrylate, collagen, alginate, Pluronic F-127, and decellularized extracellular matrix, with a succinct overview provided in a tabular format highlighting commercially available bioinks. Furthermore, concrete examples illustrating microfluidic devices and bio-printed tissues tailored for different organs, including the lung, liver, heart, and intestine, are presented. Finally, the discourse concludes with an analysis of the prospects and potential applications of microfluidics in advancing biomedical research and its practical implementations.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00371"},"PeriodicalIF":0.0,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142703235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of hydroxyapatite particle shape, and concentration on the engineering performance and printability of polycaprolactone-hydroxyapatite composites in bioplotting","authors":"Markos Petousis ,&nbsp;Vassilis Papadakis ,&nbsp;Amalia Moutsopoulou ,&nbsp;Mariza Spiridaki ,&nbsp;Apostolos Argyros ,&nbsp;Evangelos Sfakiotakis ,&nbsp;Nikolaos Michailidis ,&nbsp;Emmanuel Stratakis ,&nbsp;Nectarios Vidakis","doi":"10.1016/j.bprint.2024.e00370","DOIUrl":"10.1016/j.bprint.2024.e00370","url":null,"abstract":"<div><div>In this study, medical poly [ε-caprolactone] (PCL) was used as the matrix material for the development of composites, with hydroxyapatite (HAp) particles with angular and spherical shapes employed as additives. Pellets of such composites were created with five different filler concentrations in the range of 0.0 up to 8.0 wt% (2.0 wt % increase). Three-dimensional (3D) specimens suitable for investigation were bioplotted using the corresponding pellets. The mechanical behavior of the samples was studied in terms of their tensile and flexural characteristics. Rheological and thermal investigations were conducted, and the morphology and chemical structure were investigated using field-emission scanning electron emission SEM and EDS spectroscopy, respectively. A μ-CT scanning course was employed to evaluate the inbound porosity and dimensional conformity of the specimens. The greatest enhancement in the engineering response of the specimens was observed at a tensile strength of 6.0 wt % PCL/angular HAp, showing a 17.0 % increase over pure PCL. The results demonstrate the potential of HAp as a reinforcing agent for polymers in medical applications using bioplotting. The key findings suggest that the shape and concentration document a significant impact on their mechanical performance.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00370"},"PeriodicalIF":0.0,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142572461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Double-crosslinked dECM bioink to print a self-sustaining 3D multi-layered aortic-like construct","authors":"Federica Potere ,&nbsp;Giovanni Venturelli ,&nbsp;Beatrice Belgio ,&nbsp;Giuseppe Guagliano ,&nbsp;Federica Boschetti ,&nbsp;Sara Mantero ,&nbsp;Paola Petrini","doi":"10.1016/j.bprint.2024.e00368","DOIUrl":"10.1016/j.bprint.2024.e00368","url":null,"abstract":"<div><div>Cardiovascular disease is the leading cause of death worldwide, with related mortality increasing from 12.1 million to 18.6 million in the past 30 years.</div><div>To address the supply limitation of autologous vascular grafts and overcome the limits of current treatment options, 3D bioprinting techniques have been investigated.</div><div>This study aimed at introducing a self-supporting and multi-layered 3D bioprinted construct as a promising alternative for large-blood vessel replacement. To this end, we developed an alginate-gelatin bioink enriched with decellularized extracellular matrix (dECM) of porcine aorta combined with a two-step crosslinking process. We investigated the feasibility of achieving structural stability and shape fidelity of the bioprinted construct over time through rheological characterization, printability tests, and degradation tests.</div><div>According to the results of rheology and printability tests, dECM-enriched bioink combined with the double-crosslinking process (internal and external crosslink) showed good printability and high shape fidelity, withstanding more than 35 layers without the need for support. Moreover, the bioprinted construct preserved its structural stability over time, retaining a wall thickness comparable to that of the native aorta. Finally, immortalized mouse fibroblasts embedded in the bioink were well adhered to the bioink and alive over time. The double-crosslinked bioink represents an impactful strategy to produce an alternative conduit with the native hierarchical structure of the large blood vessels.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00368"},"PeriodicalIF":0.0,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142539888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution, integration, and challenges of 3D printing in pharmaceutical applications: A comprehensive review","authors":"Jyoti Kumari ,&nbsp;Shalini Pandey ,&nbsp;Krishna Kant Jangde ,&nbsp;Palanirajan Vijayaraj Kumar ,&nbsp;Dinesh Kumar Mishra","doi":"10.1016/j.bprint.2024.e00367","DOIUrl":"10.1016/j.bprint.2024.e00367","url":null,"abstract":"<div><div>Three-dimensional (3D) printing involves fabricating objects from digital designs by sequentially layering materials along the X, Y, and Z axes. Although this technology has existed since the 1960s, its adoption in the pharmaceutical industry remains limited. This review examines the evolution of 3D printing and its emerging significance in pharmaceuticals. The technique offers numerous advantages, such as product customization, cost-effectiveness, and efficient material usage. Several methods—such as inkjet printing, extrusion printing, and beam-based printing—are employed, utilizing materials ranging from lactose and hydroxypropyl methylcellulose to bioinks like chitosan and hyaluronic acid. Among these techniques, fused deposition modelling (FDM) is particularly noteworthy for its versatility in both biodegradable and non-biodegradable applications. Advances in 3D printing have paved the way for innovative pharmaceutical uses, including the production of complex oral dosage forms, drug delivery systems, and medical devices such as prosthetics. More recent breakthroughs have extended into bioprinting, organ-on-a-chip technologies, and robotics. However, several challenges hinder broader adoption, including limited compatibility with thermosensitive materials, difficulties in scaling production, and maintaining quality control. Additionally, the lack of standardized regulatory and ethical frameworks for clinical approval complicates progress. This review explores the key 3D printing techniques, materials, and trends relevant to pharmaceuticals, while addressing resource constraints, intellectual property issues, and regulatory hurdles. It concludes by identifying future directions for research and development, emphasizing the need to optimize these technologies for widespread pharmaceutical applications.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00367"},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pioneering bone regeneration: A review of cutting-edge scaffolds in tissue engineering","authors":"Y. Alex ,&nbsp;Sumi Vincent ,&nbsp;Nidhin Divakaran ,&nbsp;U.T. Uthappa ,&nbsp;Parthasarathy Srinivasan ,&nbsp;Suhail Mubarak ,&nbsp;Mamdouh Ahmed Al-Harthi ,&nbsp;Duraisami Dhamodharan","doi":"10.1016/j.bprint.2024.e00364","DOIUrl":"10.1016/j.bprint.2024.e00364","url":null,"abstract":"<div><div>Bone tissue engineering (BTE) is aims to develop advanced strategies to regenerate damaged or diseased bone, through the integration of principles from cellular biology, biomaterials science, and engineering. The vital aspect of these studies includes the design and fabrication of scaffolds that support cell adhesion, proliferation, and differentiation, ultimately promoting the formation of new bone tissue. Recent developments in scaffold materials have focused on organic, inorganic, and composite biomaterials. Each of these showcasing unique and distinct advantages in terms of biocompatibility, biodegradability, and mechanical strength. Polymers, such as poly (lactic-co-glycolic acid) (PLGA), provide flexibility and degradation profiles, which are conducive to tissue integration. While ceramics, including hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP), offer mechanical properties similar to native bone. The fusion of organic and inorganic components in composites has yielded scaffolds with enhanced functionality, such as improved osteo-conductivity and controlled degradation rates. Advanced fabrication techniques, particularly electrospinning and 3D printing, have revolutionized scaffold design by enabling precise control over pore size, porosity, and surface architecture, critical parameters for mimicking the extracellular matrix (ECM) of bone. These structural characteristics directly influence cellular behaviors such as migration, proliferation, and differentiation, which are crucial for successful bone regeneration. This review critically evaluates the recent advances in biomaterials for scaffold fabrication, with a focus on optimizing the interplay between material properties and scaffold architecture to improve therapeutic outcomes in bone regeneration. The findings underscore the importance of material selection and scaffold design in BTE and provide actionable insights for both researchers and clinicians in the development of next-generation scaffolds. By synthesizing recent progress in this field, the review highlights potential avenues for future research aimed at refining scaffold materials and fabrication techniques to enhance bone regeneration.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00364"},"PeriodicalIF":0.0,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D bioprinted GelMA scaffolds for clinical applications: Promise and challenges","authors":"Soumitra Das,&nbsp;Remya Valoor,&nbsp;Jeyapriya Thimukonda Jegadeesan,&nbsp;Bikramjit Basu","doi":"10.1016/j.bprint.2024.e00365","DOIUrl":"10.1016/j.bprint.2024.e00365","url":null,"abstract":"<div><div>The promise of bioprinting in diverse applications ranging from <em>in vitro</em> drug screening to creating patient-specific tissues/organs for personalized medicine has attracted significant attention globally. In this context, this review discusses the progress being made over the last decades with gelatin methacryloyl (GelMA) as a foundational hydrogel for diverse bioprintable ink formulations in particular relevance to printability, buildability, and bio-functionality. Furthermore, a comprehensive analysis is presented on the recent developments in 3D (bio)printing of GelMA for the reconstruction or regeneration of artificial tissues, spanning musculoskeletal, neurological, cardiovascular, urological, ophthalmological, dermatological, and drug screening of cancer-related applications. While presenting such wide-ranging potential, an emphasis is placed on addressing the key challenges associated with scaling up from small-scale laboratory practices to clinical applications. Furthermore, the review sheds light on the regulatory framework-related issues that impede the widespread clinical usage of 3D bioprinted tissues and organs in patient care. Taken together, this review provides significant insights into the current state of the field for researchers, clinicians, and policymakers, while navigating the intricate landscape of 3D (bio)printing for clinical translation.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"44 ","pages":"Article e00365"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142554305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A natural composite hydrogel laden with mesenchymal stromal cells for osteochondral repair: Comparison between casting and 3D bioprinting","authors":"Marjorie Dufaud ,&nbsp;Christophe Marquette ,&nbsp;Christian Jorgensen ,&nbsp;Emeline Perrier-Groult ,&nbsp;Danièle Noël","doi":"10.1016/j.bprint.2024.e00366","DOIUrl":"10.1016/j.bprint.2024.e00366","url":null,"abstract":"<div><div>Synovial joints, and particularly the osteochondral unit, are prone to lesions, with high risk of degeneration towards osteoarthritis. Various treatment strategies have been developed, including surgical techniques and cellular therapies, but they all show limitations. In this context, tissue engineering approaches, particularly 3D bioprinting, are promising for generating osteochondral tissue substitutes for joint repair. In this work, two biofabrication techniques, casting and extrusion-based 3D bioprinting, of an optimized formulation of a gelatin/alginate/fibrinogen bioink loaded with murine mesenchymal stromal cells (MSCs) were compared for the generation of cartilage and bone substitutes. Cell viability, proliferation and differentiation were characterized. Both techniques showed similar results in terms of viability and proliferation, but only the 3D bioprinted constructs allowed for differentiation towards the chondrogenic or osteogenic lineage using specific culture media. Bioprinting of biphasic osteochondral constructs comprising a cartilage compartment on top of a bone compartment was also explored. The study highlights the potential of our natural composite hydrogel bioink and extrusion-based 3D bioprinting for the generation of osteochondral tissue substitutes. Although further optimizations are needed, the study laid the groundwork for future advancements in osteochondral tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00366"},"PeriodicalIF":0.0,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D and 4D printed materials for cardiac transplantation: Advances in biogenerative engineering","authors":"Aayush Prakash ,&nbsp;Sathvik Belagodu Sridhar ,&nbsp;Adil Farooq Wali ,&nbsp;Sirajunisa Talath ,&nbsp;Javedh Shareef ,&nbsp;Rishabha Malviya","doi":"10.1016/j.bprint.2024.e00362","DOIUrl":"10.1016/j.bprint.2024.e00362","url":null,"abstract":"<div><div>The most common reason for death worldwide is cardiovascular problems, and current treatments including medication, surgery, and heart transplants have disadvantages. Both 3D and 4D printing technologies are being investigated due to the demand for sophisticated solutions in cardiac care. With the use of these technologies, it may be possible to construct intricate circulatory systems, provide individualized care, and find solutions to problems like organ shortages and immune rejection. The paper focuses on various bioprinting methods that may be used in cardiac tissue engineering to create biomimetic structures, improve vascularization, and construct functional heart tissues using 3D and 4D manufacturing. The advancement of 3D and 4D printing procedures has led to substantial advancements in heart tissue engineering by offering precise and customized solutions. These technologies make it possible to fabricate intricate cardiovascular models along with medical equipment, which improves surgical planning and allows for patient-specific therapies. There are still challenges to be solved, primarily in the areas of realistic vascularization and the use of biomaterials that resemble natural cardiac tissue in terms of their mechanical and chemical properties. Technologies for 3D and 4D printing hold promise for resolving major issues with heart transplantation, namely donor scarcity and rejection. Improving vascularization along with biomaterial incorporation for therapeutic applications has to be the main goal of future research.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00362"},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of toxicity testing platforms from 2D to advanced 3D bioprinting for safety assessment of drugs","authors":"Rohin Shyam ,&nbsp;Rinni Singh ,&nbsp;Mukul Bajpai ,&nbsp;Arunkumar Palaniappan ,&nbsp;Ramakrishnan Parthasarathi","doi":"10.1016/j.bprint.2024.e00363","DOIUrl":"10.1016/j.bprint.2024.e00363","url":null,"abstract":"<div><div>The process involved in the discovery of novel drugs in medical sciences is challenging due to the time-intensive process that results in a high cost of development. Additionally, it is reported that 90 % of new drugs fail in clinical trials and cannot reach the market. One of the primary reasons for failure is that research laboratories and pharmaceutical companies have been relying exclusively on data derived from animal-based models for testing the efficacy and safety of newly developed drugs. These models do not completely recapitulate human physiology or pathophysiology, resulting in a lower translational rate. Further, the evaluation of toxicity of drugs to the human body requires a more robust and holistic approach. Researchers across the globe are focusing on developing <em>in vitro</em>3D models as alternatives to traditional animal testing to circumvent these challenges. These model systems could replicate and mimic the human physiological microenvironment, cellular interactions, and arrangements. <em>In vitro</em>3D models would provide improved methods to evaluate and comprehend drug response, thereby reducing the burden on animal usage. Further, reducing the time and costs associated with developing, screening, drug failure, and translation of drugs is also realizable. In this communication, existing <em>in vitro</em> 3D models that are used in the drug development process are reviewed. In addition, the advancements in using 3D bioprinting and organ-on-a-chip technologies towards generating human reconstructed tissues/organs are also highlighted. The challenges from a technological and regulatory perspective on adapting these alternate animal models are also discussed.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00363"},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robust design optimization of Critical Quality Indicators (CQIs) of medical-graded polycaprolactone (PCL) in bioplotting","authors":"Nectarios Vidakis ,&nbsp;Markos Petousis ,&nbsp;Constantine David ,&nbsp;Dimitrios Sagris ,&nbsp;Nikolaos Mountakis ,&nbsp;Mariza Spiridaki ,&nbsp;Amalia Moutsopoulou ,&nbsp;Nektarios K. Nasikas","doi":"10.1016/j.bprint.2024.e00361","DOIUrl":"10.1016/j.bprint.2024.e00361","url":null,"abstract":"<div><div>Polycaprolactone (PCL), either in its pure grade or as a polymeric matrix for bio-composites, plays a key role in the biomedical and bioengineering industries. It is also considered a multifunctional and versatile polymer for bioprinting and bioplotting purposes, especially in tissue engineering. Herein, an undiscovered yet valuable aspect of PCL extrusion-based bioprinting, such as the predictability of Critical Quality Indicators (CQIs), is investigated in depth. With the aid of the robust L25 orthogonal matrix design, the six most generic and device-independent control factors proved their impact on quality metrics such as global porosity, dimensional conformity, and surface roughness, determined with the aid of highly evolved Nondestructive Testing (NDT) and algorithms. To this end, 25 experimental runs were set, and 125 specimens were fabricated using an industrial-scale bio-plotter and medical-graded polycaprolactone. Various infill densities (ID), layer thicknesses (LT), raster deposition angles (RDA), printing speeds (PS), nozzle temperatures (NT), and bed temperatures (BT) were applied. CQIs were determined using optical profilometry and microscopy, and micro-computed tomography. Quadratic predictive equations were compiled and verified using two additional, well-chosen experimental runs. These generally applicable predictive models carry a massive amount of research and industrial merit, as they ensure visibility in bioprinting with PCL.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"43 ","pages":"Article e00361"},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}