Bioprinting最新文献

{"title":"Design and simulating lattice structures in the FE analysis of the femur bone","authors":"Pourya Bazyar ,&nbsp;Ehsan Sheidaee","doi":"10.1016/j.bprint.2023.e00326","DOIUrl":"10.1016/j.bprint.2023.e00326","url":null,"abstract":"<div><p>Bone tissue engineering (BTE) research has reached a significant level of maturity. This paper reviews the role of modeling and simulation in BTE, highlighting their exceptional utility in assessing and validating experiments conducted in vitro and in vivo. The study categorizes BTE simulations into three key areas: 1- Modeling Physical Phenomena: This includes simulations based on Computer-Aided Design (CAD), medical imaging, and the finite element method. 2- Structural Complexity and Scaffold Optimization: This involves exploring intricate scaffold structures and optimizing their design. 3- Diverse Simulation Conditions for Lattice structure: This category delves into simulations under varying conditions to understand scaffold behavior. The paper's focus is on CAD-based and medical image-based finite element analysis models of lattice structure, emphasizing their importance in BTE. Two significant findings emerge: 1- In silico experiments offer extraordinary possibilities and economic benefits in BTE research. They provide invaluable insights and reduce the need for resource-intensive physical experiments. 2- Collaborative practices are crucial for advancing BTE research. Collaboration among researchers strengthens the credibility and applicability of quantifiable and structurally sound methodologies within the field, fostering innovation and progress.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"37 ","pages":"Article e00326"},"PeriodicalIF":0.0,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405886623000696/pdfft?md5=d12354bd010e19bf3ae4c09da34851dd&pid=1-s2.0-S2405886623000696-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139023117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioreactor design-assisted bioprinting of stimuli-responsive materials for tissue engineering and drug delivery applications","authors":"Amirreza Moheb Afzali ,&nbsp;Mohammad Amin Kheradmand ,&nbsp;Seyed Morteza Naghib","doi":"10.1016/j.bprint.2023.e00325","DOIUrl":"https://doi.org/10.1016/j.bprint.2023.e00325","url":null,"abstract":"<div><p>Bioreactors are essential tools in tissue engineering and drug delivery research, providing controlled environments for cell growth, tissue development, and optimization of manufacturing parameters. There are various types of bioreactors, including static, dynamic, perfusion, and rotating systems, each offering unique advantages depending on the application. Key design considerations for bioreactors include the size, geometry, components, materials, and operating conditions needed to support the cultured tissue or organ. Stimuli-responsive materials have emerged as essential components in the design of bioreactors and the fabrication of scaffolds for various applications in tissue engineering and drug delivery. These intelligent materials possess the ability to modulate their properties and functionalities in direct response to external stimuli such as temperature, pH, light, electric or magnetic fields, and biochemical signals. This inherent responsiveness affords precise control over the spatiotemporal manipulation of physical and chemical cues, thereby influencing cellular behavior and facilitating controlled release of therapeutic agents. Commonly employed stimuli-responsive polymers encompass thermoresponsive, pH-responsive, light-responsive, and redox-responsive materials.3D printing techniques allow fabrication of complex, customized scaffolds using digital designs and living cell-laden bio-inks. Bioprinting combined with stimuli-responsive materials enables 4D printing of dynamic scaffolds that transform over time when triggered. Ongoing research aims to optimize bioreactor design, develop novel smart biomaterials, achieve multi-material 4D printing, and enhance responsiveness to internal stimuli for advanced tissue engineering and drug delivery applications.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"37 ","pages":"Article e00325"},"PeriodicalIF":0.0,"publicationDate":"2023-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405886623000684/pdfft?md5=6fdfbe3d651e649e2d9779bd505c71f5&pid=1-s2.0-S2405886623000684-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138739296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular-matrix CompositeBioink for 3D bioprinting and molding of small diameter vascular graft","authors":"Kishor R. Tardalkar, Leena R Chaudhari, Mrunal N. Damle, Akshay A. Kawale, Nilesh C. Bhamare, Jeevitaa R. Kshersagar, Tanvee S. Kulkarni, M. Joshi","doi":"10.1016/j.bprint.2023.e00300","DOIUrl":"https://doi.org/10.1016/j.bprint.2023.e00300","url":null,"abstract":"","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48063545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Printability assessment workflow of thermosensitive photocurable biomaterial ink for microextrusion bioprinting","authors":"Miranda Torre, S. Giannitelli, E. Mauri, M. Gori, A. Bucciarelli, P. Mozetic, G. Gigli, M. Trombetta, A. Rainer","doi":"10.1016/j.bprint.2023.e00262","DOIUrl":"https://doi.org/10.1016/j.bprint.2023.e00262","url":null,"abstract":"","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46776714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular 3D bioprinting: A review on cardiac tissue development","authors":"Dianoosh Kalhori ,&nbsp;Nima Zakeri ,&nbsp;Mahshid Zafar-Jafarzadeh ,&nbsp;Lorenzo Moroni ,&nbsp;Mehran Solati-Hashjin","doi":"10.1016/j.bprint.2022.e00221","DOIUrl":"10.1016/j.bprint.2022.e00221","url":null,"abstract":"<div><p><span><span>Cardiovascular diseases such as myocardial infarction account for millions of worldwide deaths annually. Cardiovascular tissues constitute a highly organized and complex three-dimensional (3D) structure that makes them hard to fabricate in a </span>biomimetic manner by conventional </span>scaffold fabrication<span><span><span> methods. 3D bioprinting has been introduced as a novel cell-based method in the last two decades due to its ability to recapitulate cell density, multicellular architecture, physiochemical environment, and </span>vascularization of biological constructs with accurate designs. This review article aims to provide a comprehensive outlook to obtain cardiovascular functional tissues from the engineering of bioinks comprising cells, hydrogels, and biofactors to bioprinting techniques and relevant biophysical stimulations responsible for maturation and tissue-level functions. Also, </span>cardiac tissue 3D bioprinting investigations and further discussion over its challenges and perspectives are highlighted in this review article.</span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00221"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49317679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated and dynamic extrusion pressure adjustment based on real-time flow rate measurements for precise ink dispensing in 3D bioprinting","authors":"Lukas Wenger ,&nbsp;Svenja Strauß ,&nbsp;Jürgen Hubbuch","doi":"10.1016/j.bprint.2022.e00229","DOIUrl":"10.1016/j.bprint.2022.e00229","url":null,"abstract":"<div><p>Extrusion-based printing relying on pneumatic dispensing systems is the most widely employed tool in bioprinting. However, standardized and reliable methods for process development, monitoring and control are still not established. Suitable printing parameters are often determined in a trial-and-error approach and neither process monitoring nor real-time adjustments of extrusion pressure to environmental and process-related changes are commonly employed. The present study evaluates an approach to introduce flow rate as a main process parameter to monitor and control extrusion-based bioprinting. An experimental setup was established by integrating a liquid flow meter between the cartridge and nozzle of a pneumatically driven bioprinter to measure the actual flow of dispensed ink in real-time. The measured flow rate was fed to a Python-based software tool implementing a proportional-integral-derivative (PID) feedback loop that automatically and dynamically adapted the extrusion pressure of the bioprinter to meet a specified target flow rate. The performance of the employed experimental setup was evaluated with three different model inks in three application examples. a) Continuous dispensing: Several runs of continuous dispensing showed that the PID-based pressure control was able to generate a steady flow rate more consistently and precisely than constant pressure settings. b) Adaptation to ink inhomogeneities: Deliberately created ink inhomogeneities were successfully compensated for by real-time pressure adjustments which profoundly enhanced the printing quality compared to printing without adaptive pressure. c) Process transfer to other nozzle types: Experiments with different nozzle types demonstrated the potential of the established setup to facilitate and accelerate process transfer and development. The present study provides an alternative approach for process design, monitoring and control by introducing flow rate as a main process parameter. We propose bioprinting processes to be based on flow rate specifications instead of constant pressure settings. This approach has the potential to save time by avoiding tedious parameter screenings and to introduce an active, real-time control over the printing process. Subjective influences by individual users during process development can be reduced and the process transfer between different devices and experimental setups can be facilitated and accelerated.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00229"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405886622000392/pdfft?md5=81aa8f79f6037b86f62e1cd612b5ce77&pid=1-s2.0-S2405886622000392-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49193647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatially guided endothelial tubulogenesis by laser-induced side transfer (LIST) bioprinting of HUVECs","authors":"Hamid Ebrahimi Orimi ,&nbsp;Erika Hooker ,&nbsp;Sivakumar Narayanswamy ,&nbsp;Bruno Larrivée ,&nbsp;Christos Boutopoulos","doi":"10.1016/j.bprint.2022.e00240","DOIUrl":"10.1016/j.bprint.2022.e00240","url":null,"abstract":"<div><p><span>The ability to bioprint microvasculature networks is central for drug screening and for </span>tissue engineering<span> applications. Here we used a newly developed bioprinting technology, termed laser-induced side transfer (LIST), to print human umbilical vein endothelial cells<span><span> (HUVECs) and to spatially guide endothelial tubulogenesis. We investigated the effect of three bioprinting matrices (fibrin, Matrigel and Matrigel/thrombin) on HUVECs self-assembly. Furthermore, we studied the effect of pro- and anti-angiogenic compounds on </span>sprouting angiogenesis<span> and tubulogenesis. We found that HUVECs self-assembly is optimal on Matrigel/thrombin due to the formation of fibrin stripes that enhance HUVECs confinement<span><span> and adhesion. Importantly, we showed that treatment of printed HUVEC lines with the anti-angiogenic factor </span>bone morphogenetic protein 9 (BMP9) significantly improves the percentage of lumen coverage. Our results showcase LIST as a powerful bioprinting technology to study tubulogenesis and to screen compounds targeting microvasculature pathologies.</span></span></span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00240"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47997607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-planar embedded 3D printing for complex hydrogel manufacturing","authors":"Benjamin J. Albert ,&nbsp;Coral Wang ,&nbsp;Christian Williams ,&nbsp;Jonathan T. Butcher","doi":"10.1016/j.bprint.2022.e00242","DOIUrl":"10.1016/j.bprint.2022.e00242","url":null,"abstract":"<div><p><span>Embedded bioprinting as a tissue engineering method has expanded the ability to bioprint complex geometry of native tissue. Print bath support in these methods allows the biomaterial to solidify in place, mitigating the possibly negative effects of low viscosity and gravity. This material stability also permits for non-planar deposition of the biomaterial. Here, we developed a non-planar 3D print slicer for non-planar embedded bioprinting. We quantified the changes in ink deposition properties with respect to non-planar movement to understand printability in the system. </span>Alginate<span> prints in a FRESH support bath were used to quantify the capability of the slicer to create tunable mechanical properties<span>. Mechanical testing reveals that geometric changes to the printed models can tune stiffness, failure stress and strain, and Poisson's ratio. These results demonstrate that using non-planar manufacturing can produce mechanically tunable properties with a homogeneous biomaterial. This may strengthen our ability to precisely match mechanical properties of native tissues to improve tissue engineering outcomes.</span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00242"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43657042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-based material formulation for extrusion printing by dityrosine crosslinking of unmodified casein","authors":"Sandra Haas,&nbsp;Friederike Götz,&nbsp;Jürgen Hubbuch","doi":"10.1016/j.bprint.2022.e00245","DOIUrl":"10.1016/j.bprint.2022.e00245","url":null,"abstract":"<div><p><span>In the development of new functional bio-based materials in the field of three-dimensional (3D) printing, visible light-induced dityrosine crosslinking gains increasing interest. In this context, most current bio-based materials and ink formulations rely on previously modified chemical substances with increased tyrosine availability. In contrast, we developed and characterized a photopolymerizable ink formulation for extrusion printing based on the unmodified and naturally occurring protein casein. Manufacturability of formulations containing protein, photoinitiating system, buffer and a thickening agent turned out to be a key factor for the ink development. In total, eight different thickening agents were assessed regarding their suitability to increase the viscosity of the ink formulation to expand the fabrication window for extrusion-based 3D printing. The </span>mechanical properties<span><span> of the ink formulation and hydrogel in presence of sodium alginate were further characterized and the macroscopic fabrication of </span>auxetic structures consisting of up to 30 layers was achieved by applying extrusion-based printing.</span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00245"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41854358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Print me a cornea - Are we there yet?","authors":"Midhun Ben Thomas ,&nbsp;Shivaram Selvam ,&nbsp;Parinita Agrawal ,&nbsp;Prayag Bellur ,&nbsp;Neha Waghmare ,&nbsp;Suvro K. Chowdhury ,&nbsp;Kamalnath Selvakumar ,&nbsp;Aastha Singh ,&nbsp;Anil Tiwari ,&nbsp;Abha Gour ,&nbsp;Virender S. Sangwan ,&nbsp;Tuhin Bhowmick ,&nbsp;Arun Chandru","doi":"10.1016/j.bprint.2022.e00227","DOIUrl":"10.1016/j.bprint.2022.e00227","url":null,"abstract":"<div><p><span>Corneal diseases<span><span> are the third most prevalent cause of blindness after cataract and glaucoma. It is estimated that about 5 million people in the world are affected by bilateral corneal blindness with an additional 23 million with </span>unilateral blindness<span>. Cornea transplantation is the standard practice for the management of various cornea related pathologies like fibrosis, </span></span></span>ulcers<span>, keratitis<span><span><span><span>, etc. The high transplant cost, increased risk of graft failure/rejection, and long waiting list due to limited availability of good quality donor cornea imposes a huge clinical burden. Recently, biofabrication<span> technologies are gaining a lot of attention because of their potential to direct hierarchical assembly of three-dimensional (3D) biological structures for tissue construction for various biomedical and clinical applications. In this regard, </span></span>3D bioprinting, which involves layer-by-layer deposition of acellular or cell-laden bioink in a specific pattern corresponding to the organotypic morphology of tissues/organs, has been extensively investigated for the fabrication of corneal substitutes. In addition to this methodology, novel biofabrication techniques have been explored for the fabrication of </span>corneal tissues using bioinks with optical and mechanical performances comparable to native </span>cornea tissue. In this review, we highlight the recent advances and offer future perspectives in the fabrication of corneal tissue equivalents that can be potentially employed for effective clinical repair, reconstruction, and regeneration of the cornea.</span></span></p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":"28 ","pages":"Article e00227"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42395194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}