Bioprinting Pub Date : 2024-10-12 DOI: 10.1016/j.bprint.2024.e00362

{"title":"3D and 4D printed materials for cardiac transplantation: Advances in biogenerative engineering","authors":"","doi":"10.1016/j.bprint.2024.e00362","DOIUrl":"10.1016/j.bprint.2024.e00362","url":null,"abstract":"<div><div>The most common reason for death worldwide is cardiovascular problems, and current treatments including medication, surgery, and heart transplants have disadvantages. Both 3D and 4D printing technologies are being investigated due to the demand for sophisticated solutions in cardiac care. With the use of these technologies, it may be possible to construct intricate circulatory systems, provide individualized care, and find solutions to problems like organ shortages and immune rejection. The paper focuses on various bioprinting methods that may be used in cardiac tissue engineering to create biomimetic structures, improve vascularization, and construct functional heart tissues using 3D and 4D manufacturing. The advancement of 3D and 4D printing procedures has led to substantial advancements in heart tissue engineering by offering precise and customized solutions. These technologies make it possible to fabricate intricate cardiovascular models along with medical equipment, which improves surgical planning and allows for patient-specific therapies. There are still challenges to be solved, primarily in the areas of realistic vascularization and the use of biomaterials that resemble natural cardiac tissue in terms of their mechanical and chemical properties. Technologies for 3D and 4D printing hold promise for resolving major issues with heart transplantation, namely donor scarcity and rejection. Improving vascularization along with biomaterial incorporation for therapeutic applications has to be the main goal of future research.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142432421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolution of toxicity testing platforms from 2D to advanced 3D bioprinting for safety assessment of drugs 用于药物安全性评估的毒性测试平台从二维发展到先进的三维生物打印技术

Bioprinting Pub Date : 2024-10-11 DOI: 10.1016/j.bprint.2024.e00363

{"title":"Evolution of toxicity testing platforms from 2D to advanced 3D bioprinting for safety assessment of drugs","authors":"","doi":"10.1016/j.bprint.2024.e00363","DOIUrl":"10.1016/j.bprint.2024.e00363","url":null,"abstract":"<div><div>The process involved in the discovery of novel drugs in medical sciences is challenging due to the time-intensive process that results in a high cost of development. Additionally, it is reported that 90 % of new drugs fail in clinical trials and cannot reach the market. One of the primary reasons for failure is that research laboratories and pharmaceutical companies have been relying exclusively on data derived from animal-based models for testing the efficacy and safety of newly developed drugs. These models do not completely recapitulate human physiology or pathophysiology, resulting in a lower translational rate. Further, the evaluation of toxicity of drugs to the human body requires a more robust and holistic approach. Researchers across the globe are focusing on developing <em>in vitro</em>3D models as alternatives to traditional animal testing to circumvent these challenges. These model systems could replicate and mimic the human physiological microenvironment, cellular interactions, and arrangements. <em>In vitro</em>3D models would provide improved methods to evaluate and comprehend drug response, thereby reducing the burden on animal usage. Further, reducing the time and costs associated with developing, screening, drug failure, and translation of drugs is also realizable. In this communication, existing <em>in vitro</em> 3D models that are used in the drug development process are reviewed. In addition, the advancements in using 3D bioprinting and organ-on-a-chip technologies towards generating human reconstructed tissues/organs are also highlighted. The challenges from a technological and regulatory perspective on adapting these alternate animal models are also discussed.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Robust design optimization of Critical Quality Indicators (CQIs) of medical-graded polycaprolactone (PCL) in bioplotting 生物印染中医用级聚己内酯（PCL）关键质量指标（CQIs）的稳健优化设计

Bioprinting Pub Date : 2024-10-05 DOI: 10.1016/j.bprint.2024.e00361

{"title":"Robust design optimization of Critical Quality Indicators (CQIs) of medical-graded polycaprolactone (PCL) in bioplotting","authors":"","doi":"10.1016/j.bprint.2024.e00361","DOIUrl":"10.1016/j.bprint.2024.e00361","url":null,"abstract":"<div><div>Polycaprolactone (PCL), either in its pure grade or as a polymeric matrix for bio-composites, plays a key role in the biomedical and bioengineering industries. It is also considered a multifunctional and versatile polymer for bioprinting and bioplotting purposes, especially in tissue engineering. Herein, an undiscovered yet valuable aspect of PCL extrusion-based bioprinting, such as the predictability of Critical Quality Indicators (CQIs), is investigated in depth. With the aid of the robust L25 orthogonal matrix design, the six most generic and device-independent control factors proved their impact on quality metrics such as global porosity, dimensional conformity, and surface roughness, determined with the aid of highly evolved Nondestructive Testing (NDT) and algorithms. To this end, 25 experimental runs were set, and 125 specimens were fabricated using an industrial-scale bio-plotter and medical-graded polycaprolactone. Various infill densities (ID), layer thicknesses (LT), raster deposition angles (RDA), printing speeds (PS), nozzle temperatures (NT), and bed temperatures (BT) were applied. CQIs were determined using optical profilometry and microscopy, and micro-computed tomography. Quadratic predictive equations were compiled and verified using two additional, well-chosen experimental runs. These generally applicable predictive models carry a massive amount of research and industrial merit, as they ensure visibility in bioprinting with PCL.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the development of stereolithography-based additive manufacturing processes: A review of applications and challenges 基于立体光刻技术的增材制造工艺的最新进展：应用与挑战综述

Bioprinting Pub Date : 2024-09-26 DOI: 10.1016/j.bprint.2024.e00360

{"title":"Recent advances in the development of stereolithography-based additive manufacturing processes: A review of applications and challenges","authors":"","doi":"10.1016/j.bprint.2024.e00360","DOIUrl":"10.1016/j.bprint.2024.e00360","url":null,"abstract":"<div><div>Additive manufacturing processes have progressed over recent years due to their superiority over conventional manufacturing methods. Their ability to fabricate materials with complex structures, increased precision, and reduced cost have opened avenues for various industrial applications, including biomedical, electrical, mechanical, aviation, and filtration, and led to their development over time. Stereolithography (SLA) is an additive manufacturing technique, through photopolymerization reaction, it solidifies a selective resin to produce three-dimensional objects. SLA has emerged as a leading 3D printing technique, revolutionizing prototyping and production across various industries. SLA has been through four generations of development and advancement, resulting in its improved performance, the diversity of materials, and the variety of applications. Stereolithography has diversified its material and emerged as a promising method for polymer-based composite when operating under optimized conditions. SLA offers superior resolution, high finish quality, improved speed and precision, and is cost-effective compared to alternative techniques like Fused Deposition Modeling (FDM). This current study aims to comprehensively review SLA development, its processes, applications and inherent challenges in mechanical, electrical and biomedical fields.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142426430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimizing biomaterial inks: A study on the printability of Carboxymethyl cellulose-Laponite nanocomposite hydrogels and dental pulp stem cells bioprinting 优化生物材料墨水：羧甲基纤维素-皂石纳米复合水凝胶和牙髓干细胞生物打印的可打印性研究

Bioprinting Pub Date : 2024-09-18 DOI: 10.1016/j.bprint.2024.e00358

{"title":"Optimizing biomaterial inks: A study on the printability of Carboxymethyl cellulose-Laponite nanocomposite hydrogels and dental pulp stem cells bioprinting","authors":"","doi":"10.1016/j.bprint.2024.e00358","DOIUrl":"10.1016/j.bprint.2024.e00358","url":null,"abstract":"<div><p>Tissue engineering approaches require biocompatible materials with precise pre-designed geometry, shape fidelity, and promote cellular functions. Addressing these requirements, our study focused on developing an optimized bioink formulation using carboxymethyl cellulose (CMC) and Laponite hydrogels tailored for extrusion-based three-dimensional bioprinting. To this, we investigated the rheological properties and filament behavior before and during printing. As Laponite concentration increased in CMC solutions, it improved shear-thinning behavior, viscosity, and storage modulus, resulting in well-defined filament characteristics with lower diffusion rates, excellent shape fidelity, and robust printability. Thus, we achieved a suitable biomaterial ink formulation with concentrations of 1 wt% of CMC and 4 wt% of Laponite (1C4L). Subsequently, a statistical analysis guided us to select the optimal parameters for large-scale construct printing: a nozzle speed of 5 mm/s, a print distance of 0.41 mm, and an extrusion multiplier of 1.35. After that, we enhanced the structural integrity of printed hydrogels through ionic crosslinking with calcium chloride (CaCl<sub>2</sub>) and citric acid (CA), revealing higher-strength hydrogels at higher concentrations of CaCl<sub>2</sub>. Finally, we have confirmed the groundbreaking potential of our bioink by integrating dental pulp mesenchymal stem cells (DPSC) into the 1C4L ink. Our bioprinted constructs showed optimized swelling, non-toxic effects, and retained excellent shape fidelity, crucial for creating anatomically accurate tissues. Our findings provide crucial insights linking the rheological analysis, the bioprinting process, and the biological properties of hydrogels, paving the way for their use for tissue engineering and other biomedical applications.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142270837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision 3D printing of chitosan-bioactive glass inks: Rheological optimization for enhanced shape fidelity in tissue engineering scaffolds 壳聚糖生物活性玻璃油墨的精密三维打印：流变学优化提高组织工程支架的形状保真度

Bioprinting Pub Date : 2024-09-18 DOI: 10.1016/j.bprint.2024.e00359

{"title":"Precision 3D printing of chitosan-bioactive glass inks: Rheological optimization for enhanced shape fidelity in tissue engineering scaffolds","authors":"","doi":"10.1016/j.bprint.2024.e00359","DOIUrl":"10.1016/j.bprint.2024.e00359","url":null,"abstract":"<div><div>3D printing technology in tissue engineering applications provides several advantages for scaffold development, especially with natural materials, such as chitosan, which provides a biomimetic environment for cellular growth. However, chitosan hydrogel-based inks still show poor printing fidelity. In this article, we overcame this challenge by incorporating bioactive glasses (BG) nanoparticles (up to 5 wt%) into the chitosan hydrogel. The resulting inks were characterized by rheological tests, while their processability was evaluated through measurements of shape fidelity. An indirect cytotoxicity assay was also conducted to evaluate the cell viability of the printed scaffolds. The results indicated that adding BG nanoparticles to the chitosan-based ink modified its rheological properties and improved its shape-fidelity during 3D printing, which we suggest are consequences of hydrogen bonds established between the glass and the chitosan chains. Also, cytotoxicity assessment demonstrated that the resulting scaffold exhibits high cell viability. In conclusion, the proposed composite ink has optimized rheological properties for 3D printing and is promising for applications in tissue engineering.</div></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advancements and challenges in 3D bioprinting for cancer applications 三维生物打印在癌症应用领域的最新进展和挑战

Bioprinting Pub Date : 2024-09-13 DOI: 10.1016/j.bprint.2024.e00357

引用次数: 0

Advanced additive manufacturing in implant dentistry: 3D printing technologies, printable materials, current applications and future requirements 种植牙领域的先进增材制造技术：三维打印技术、可打印材料、当前应用和未来需求

Bioprinting Pub Date : 2024-08-24 DOI: 10.1016/j.bprint.2024.e00356

{"title":"Advanced additive manufacturing in implant dentistry: 3D printing technologies, printable materials, current applications and future requirements","authors":"","doi":"10.1016/j.bprint.2024.e00356","DOIUrl":"10.1016/j.bprint.2024.e00356","url":null,"abstract":"<div><p>The utilization of 3D printing technologies is extensively pervasive across diverse sectors, including design, engineering, and manufacturing. These sophisticated manufacturing techniques depend on digitally designed models to autonomously construct 3D objects. With the growing interest in 3D printing within dentistry, specifically regarding dental implants, there has been a rapid dissemination of information pertaining to this domain and its applications. As a result, it has become crucial to conduct a comprehensive review on this topic. 3D printing technologies have played a pivotal role in oral implantology. This review provides a comprehensive analysis of the current state and future needs of 3D printing in implant dentistry, covering technologies, printable materials, and applications in both the surgical and prosthodontic stages of dental implant therapy. Furthermore, it discusses considerations for choosing the appropriate 3D printing technology for specific dental applications. This comprehensive examination offers key insights into the progress, practical uses, and future prospects of 3D printing in dental implants.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405886624000289/pdfft?md5=5604ceec5d3673820740d64f67eac60d&pid=1-s2.0-S2405886624000289-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of current state-of-the-art materiobiology and technological approaches for liver tissue engineering 综述当前最先进的肝脏组织工程材料生物学和技术方法

Bioprinting Pub Date : 2024-08-23 DOI: 10.1016/j.bprint.2024.e00355

{"title":"A review of current state-of-the-art materiobiology and technological approaches for liver tissue engineering","authors":"","doi":"10.1016/j.bprint.2024.e00355","DOIUrl":"10.1016/j.bprint.2024.e00355","url":null,"abstract":"<div><p>Chronic liver disease and related disorders are responsible for millions of deaths each year worldwide. In clinical practice, liver transplantation is recognized as an effective means of saving the lives of patients with severe complications. The shortage of organ donors has necessitated the development of bioengineered therapies that promote regeneration of the defective site and the creation of closely mimicking in vitro models for early prediction of disease states, hepatotoxicity testing, and accurate diagnostics. Despite tremendous research efforts, bioengineering of fully functional livers, detailed information on rare pathological mechanisms, and reliable bioartificial tissue-based therapies remain limited. On the other hand, 2D monolayer culture techniques are too simple to mimic and reproduce the functional characteristics of the liver accurately, its structural microenvironment, and the dynamic situation of cells in vivo. Therefore, tissue engineering-based 3D constructs outperform 2D culture systems. In this review, we provide insight into liver-related health complications, and the use of different cell types for tissue engineering. We also assess the current state of materiobiology and bioengineering technologies for fabricating 3D constructs. Afterward, we highlight the recent progress in liver tissue engineering, and outline the most relevant studies applying co-culture systems, spheroids, and organoid approaches, microfluidics, and 3D-bioprinting techniques. Finally, current dilemmas and possible future directions are explored.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142137129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing mechanical performance of solvent-cast 3D printed PCL composites: A comprehensive optimization approach 提高溶剂铸造 3D 打印 PCL 复合材料的机械性能：综合优化方法

Bioprinting Pub Date : 2024-08-14 DOI: 10.1016/j.bprint.2024.e00354

{"title":"Enhancing mechanical performance of solvent-cast 3D printed PCL composites: A comprehensive optimization approach","authors":"","doi":"10.1016/j.bprint.2024.e00354","DOIUrl":"10.1016/j.bprint.2024.e00354","url":null,"abstract":"<div><p>This study aims to enhance the mechanical properties of 3D-printed scaffolds by optimizing a composite of Poly-ε-caprolactone (PCL), poly-hydroxybutyrate (PHB), and synthetic fluorapatite (FHAp) using Response Surface Methodology (RSM). The research targets the intricate relationships between PCL, PHB, and FHAp concentrations, crucial for achieving optimal tensile, compressive, and flexural strengths. The solvent-cast process successfully yielded FHAp-reinforced PCL composites, confirmed by XRD and FTIR spectra. The findings indicate that an optimal PHB content of over 15 % wt/v and PCL under 10 % wt/v significantly enhance tensile strength, achieving values up to 48 MPa. Compressive strength peaked at PHB concentrations of 13–16 % wt/v and PCL concentrations of 9–13 % wt/v, showcasing effective stress transmission, with the highest recorded value being 90 MPa. Flexural strength exceeded 100 MPa with lower concentrations of PCL and PHB, emphasizing the need for a balance of rigidity and flexibility. The study identifies the optimum composition for these mechanical properties at PCL 9.432 % wt/v, PHB 16.568 % wt/v, and FHAp 24.933 % wt/v, crucial for advanced biomedical implant applications.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141997553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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