Multi-material 3D bioprinting of human stem cells to engineer complex human corneal structures with stroma and epithelium

Q1 Computer Science
P. Puistola , S. Huhtanen , K. Hopia , S. Miettinen , A. Mörö , H. Skottman
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引用次数: 0

Abstract

Developing cost-effective and scalable multi-material bioprinting technologies that combine multiple cell types is crucial to produce biomimetic, complex human tissue substitutes and overcome the scarcity of transplantable tissues. These technological developments can revolutionize the treatment of several conditions currently dependent on donor tissues, such as corneal blindness. Here, corneal structures consisting of two layers, stroma and epithelium, were manufactured by extrusion-based 3D bioprinting. To take steps towards clinical translation of bioprinting, three clinically compatible hyaluronic acid based bioinks were combined with human adipose tissue and induced pluripotent stem cell derived cell types. Each of the three bioinks was customized to suit the needs of different cells and to provide mechanical stability for the bioprinted structure. Along with offering a 3D environment with excellent cytocompatibility, these bioprinted corneal structures facilitated cellular interactions and network formation, which are essential for creating functional tissue substitutes. Consequently, this study provides important insight on how to bring together the technical aspects of multi-material bioprinting as well as the biological relevance and scalability of the bioprinted constructs, advancing the field of additive manufacturing for clinical applications.

Abstract Image

人类干细胞的多材料3D生物打印工程复杂的人角膜结构与基质和上皮
开发结合多种细胞类型的具有成本效益和可扩展的多材料生物打印技术对于生产仿生、复杂的人体组织替代品和克服可移植组织的稀缺性至关重要。这些技术的发展可以彻底改变目前依赖于供体组织的几种疾病的治疗,例如角膜失明。在这里,角膜结构由两层组成,间质和上皮,是通过挤压生物3D打印制造的。为了实现生物打印的临床转化,我们将三种临床兼容的透明质酸生物墨水与人类脂肪组织和诱导多能干细胞来源的细胞类型结合起来。三种生物墨水中的每一种都是定制的,以适应不同细胞的需要,并为生物打印结构提供机械稳定性。除了提供具有优异细胞相容性的3D环境外,这些生物打印的角膜结构促进了细胞相互作用和网络形成,这对于创建功能性组织替代品至关重要。因此,这项研究为如何将多材料生物打印的技术方面以及生物打印结构的生物学相关性和可扩展性结合在一起提供了重要的见解,推动了临床应用的增材制造领域。
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来源期刊
Bioprinting
Bioprinting Computer Science-Computer Science Applications
CiteScore
11.50
自引率
0.00%
发文量
72
审稿时长
68 days
期刊介绍: Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.
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