Fabrication of organ-on-a-chip using microfluidics

Q1 Computer Science

Bioprinting Pub Date : 2025-01-22 DOI:10.1016/j.bprint.2025.e00394

S. Ying-Jin , I. Yuste , E. González-Burgos , D.R. Serrano

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Abstract

The use of microfluidic devices represents a significant advancement beyond conventional techniques in the development of innovative in vitro assays. Microfluidic chips are specialized devices that precisely control fluids at the microscale level through intricate microchannels, enabling the replication of physical and chemical conditions. When combined with tissue engineering, these chips have evolved into highly specialized tools known as Organ-on-a-Chip (OoC) devices, which can simulate the physiology and functionality of various human tissues and organs. OoC devices are cutting-edge technologies that integrate a biological component representing the target organ with a microfluidic component that mimics blood flow. This combination allows for the replication of biological structures with a more accurate representation of the in vivo physiological cellular microenvironment, which can be finely tuned by adjusting the flow rate and composition. As a result, novel microfluidic models for in vitro research can overcome the limitations of traditional 2D and 3D static cell cultures, enabling faster clinical translation and more precise predictions of the efficacy, safety, pharmacodynamics, and pharmacokinetics of new drugs. This review will discuss various techniques for fabricating OoCs and their applications in mimicking different physiological microenvironments.

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来源期刊

Bioprinting Computer Science-Computer Science Applications

CiteScore

11.50

自引率

0.00%

发文量

审稿时长

68 days

期刊介绍： Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.