Current Research in Microbial Sciences最新文献

{"title":"Advancement in the development of gene/protein-based vaccines against African swine fever virus","authors":"Ning Wang ,&nbsp;Pan Huang ,&nbsp;Jun Zhang ,&nbsp;Minqi Lin ,&nbsp;Xiaoru Lai ,&nbsp;Jianwen Chen ,&nbsp;Chungen Pan","doi":"10.1016/j.crmicr.2024.100232","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100232","url":null,"abstract":"<div><p>African swine fever (ASF) is a highly contagious acute hemorrhagic viral disease, with the mortality rate of up to 100 % in domestic pigs. In recent years, ASF outbreaks have caused huge economic losses in numerous countries and regions, especially in Asia. Therefore, there is a pressing need to develop safe and effective vaccines against infection of the causative pathogen, African swine fever virus (ASFV). ASFV contains a large genome composed of double-stranded DNA with a size of 170–194 kb, which encodes nearly 200 viral proteins. Understanding the function of these complex genes/proteins and their roles in the generation of protective immunity will help in the development of ASFV vaccines. In this article, the gene/protein-based vaccine candidate are summarized, and the structural proteins which have been previously reported to protect animals from the virus challenge were emphatically described.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100232"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000142/pdfft?md5=577c2c1d51722e2a55abf9ee21b17e8e&pid=1-s2.0-S2666517424000142-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140160678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphing the Green route: Enzymatic hydrolysis in sustainable decomposition","authors":"Rajat Singh ,&nbsp;Rajul Jain ,&nbsp;Priyanka Soni ,&nbsp;Sergio de los Santos-Villalobos ,&nbsp;Sourav Chattaraj ,&nbsp;Deblina Roy ,&nbsp;Debasis Mitra ,&nbsp;Ashish Gaur","doi":"10.1016/j.crmicr.2024.100281","DOIUrl":"10.1016/j.crmicr.2024.100281","url":null,"abstract":"<div><div>This graphical review article explores how sustainable decomposition contributes to environmental sustainability in waste management with a focus on enzymatic hydrolysis. Methods such as composting and anaerobic digestion efficiently break down organic waste and reduce landfill use and greenhouse gas emissions, while producing valuable resources such as compost and biogas. In particular, enzymatic hydrolysis offers advantages over chemical methods because it operates under mild conditions, targets specific substrates precisely, and yields purer products with fewer side reactions. Its renewable and biodegradable nature aligns with sustainability goals, making it suitable for waste decomposition, biorefining, and resource recovery. Enzymatic waste conversion reduces waste and pollution, conserves natural resources, and supports circular economy. Various ongoing studies have aimed to enhance the efficiency and environmental benefits of enzymatic hydrolysis, enabling innovative waste-to-value solutions that address environmental, economic, and social challenges. This article emphasizes the importance of its timely examination of enzymatic hydrolysis as a prominent method for sustainable waste decomposition, stressing its environmental, economic, and societal benefits. It distinguishes itself through its extensive analysis of chemical methods, its emphasis on the circular economy, and its delineation of future research directions and the need for interdisciplinary collaboration to advance this innovative technology.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100281"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revalorization of degraded maguey pulquero substrate for Lycopersicon esculentum germination","authors":"B.S. Velázquez-De Lucio ,&nbsp;E.M. Hernández-Domínguez ,&nbsp;M.P. Falcón-León ,&nbsp;A. Téllez-Jurado ,&nbsp;J Álvarez-Cervantes","doi":"10.1016/j.crmicr.2024.100283","DOIUrl":"10.1016/j.crmicr.2024.100283","url":null,"abstract":"<div><div>The bagasse of <em>Agave salmiana</em> (maguey pulquero) is a residue generated during the exploitation of the plant to obtain pulque, inulin, honey, etc. Due to its chemical composition, it can be used for the cultivation of fungi of the <em>Pleurotus</em> genus and the subsequent use of the degraded material \"degraded substrate (DS)\" as a support for the germination of vegetables. The objective of the study was to characterize the bagasse of maguey pulquero biodegraded by <em>Pleurotus djamor</em> as a new perspective in its value chain, and subsequent use for the germination of <em>Lycopersicon esculetum</em> (tomato). The DS was recovered at 60 d from the <em>P. djamor</em> culture, characterized physicochemically and the conformation of the plant tissue was observed by scanning electron microscopy. The DS showed a decrease in protein (4.8–3.3 %) content and fibrous fraction (54–36 %), but dry matter digestibility increased from 47 to 71 %; in addition, changes in mineral composition were observed, mainly in calcium concentration (6 %). Due to its composition, it is possible to revalue DS in the germination of L. <em>esculetum</em> to reduce the use of peat moss (commercial peat). The results show that up to 25 % of maguey DS mixed with 75 % peat moss can be used (25:75), reaching a germination percentage of 85 % and increasing the seedling emergence speed index from 0.96 – 1.25. Concluding that it is possible to implement a circular strategy in which agave bagasse is used for mushroom cultivation and the subsequent recovery of the spent substrate for tomato germination.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100283"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a luminescence-based method for measuring West Nile Virus MTase activity and its application to screen for antivirals","authors":"Alejandra Álvarez-Mínguez ,&nbsp;Natalia del Río ,&nbsp;Ana Belén-Blázquez ,&nbsp;Elena Casanova ,&nbsp;José-María Orduña ,&nbsp;Patricia Camarero ,&nbsp;Carolina Hurtado-Marcos ,&nbsp;Carmen del Águila ,&nbsp;María–Jesús Pérez-Pérez ,&nbsp;Miguel A. Martín-Acebes ,&nbsp;Rubén Agudo","doi":"10.1016/j.crmicr.2024.100282","DOIUrl":"10.1016/j.crmicr.2024.100282","url":null,"abstract":"<div><div>West Nile virus (WNV) is a flavivirus responsible for causing febrile illness and severe neurological diseases, with an increasing impact on human health around the world. However, there is still no adequate therapeutic treatment available to struggle WNV infections. Therefore, there is an urgent need to develop new techniques to accelerate the discovery of drugs against this pathogen. The main protein implicated in the replication of WNV is the non-structural protein 5 (NS5). This multifunctional protein contains methyltransferase (MTase) activity involved in the capping formation at the 5′-end of RNA and the methylation of internal viral RNA residues, both functions being essential for viral processes, such as RNA translation and escape from the innate immune response.</div><div>We have developed a straightforward luminescence-based assay to monitor the MTase activity of the WNV NS5 protein with potential for high-throughput screening. We have validated this method as a sensitive and suitable assay for the identification of WNV MTase inhibitors assessing the inhibitory effect of the broad MTase inhibitor sinefungin, a natural nucleoside analog of the universal methyl donor S-adenosyl methionine (SAM). The screening of a small series of purine derivatives identified an adenosine derivative as a dose-dependent inhibitor of the MTase activity. The antiviral efficacy of this compound was further confirmed in WNV infections, displaying a measurable antiviral effect. This result supports the utility of this novel method for the screening of inhibitors against WNV MTase activity, which can be of special relevance to the discovery and development of therapeutics against WNV.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100282"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photosynthetic activity and growth of poblano pepper biofertilized with plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi","authors":"Apolinar González-Mancilla ,&nbsp;Juan José Almaraz-Suárez ,&nbsp;Ronald Ferrera-Cerrato ,&nbsp;María del Pilar Rodríguez-Guzmán ,&nbsp;Oswaldo Rey Taboada-Gaytán","doi":"10.1016/j.crmicr.2024.100269","DOIUrl":"10.1016/j.crmicr.2024.100269","url":null,"abstract":"<div><p>The rhizosphere of plants are natural hosts for beneficial microorganisms such as plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF). The objective of this work was to determine the effect of a consortium of AMF and three strains of PGPR on growth, gas exchange and phosphorus content in poblano pepper plants. An experiment was established in a completely randomized design with factorial arrangement, with two factors: AMF [<em>Funneliformis geosporum</em> and <em>Claroideoglomus</em> sp. (AM) and without AM (WM)] and PGPR [<em>Rhizobium nepotum</em> (B1), <em>Serratia plymuthica</em> (B2), <em>Pseudomonas tolaasii</em> (B3) and without PGPR (WB)]; generating eight treatments: T1) AM+B1, T2) AM+B2, T3) AM+B3, T4) AM+WB, T5) WM+B1, T6) WM+B2, T7) WM+B3 and T8) WM+WB. Plant height, number of leaves, leaf area, number of flowers, dry biomass, phosphorus content and AMF colonization were measured; internal CO₂ concentration (Ci), transpiration rate (E), stomatal conductance (gS) and photosynthesis rate (Pn) were determined in leaves. Co-inoculation with AM+B3 promoted greater height (35%), number of leaves (66%), leaf area (62%), dry biomass (140%), phosphorus content (195%) and mycorrhizal colonization (26%); AM+B2 improved Ci (5%), E (8%), gS (5%) and Pn (9%) in poblano pepper leaves, compared to the control treatment (WM+WB). Biofertilization with AMF and PGPR improved gas exchange and growth of poblano pepper.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100269"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000518/pdfft?md5=50039a96de11bdfd7ff4eaf3349a30f9&pid=1-s2.0-S2666517424000518-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142099557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cutting edge tools in the field of soil microbiology","authors":"Diksha Garg ,&nbsp;Niketan Patel ,&nbsp;Anamika Rawat ,&nbsp;Alexandre Soares Rosado","doi":"10.1016/j.crmicr.2024.100226","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100226","url":null,"abstract":"<div><p>The study of the whole of the genetic material contained within the microbial populations found in a certain environment is made possible by metagenomics. This technique enables a thorough knowledge of the variety, function, and interactions of microbial communities that are notoriously difficult to research. Due to the limitations of conventional techniques such as culturing and PCR-based methodologies, soil microbiology is a particularly challenging field. Metagenomics has emerged as an effective technique for overcoming these obstacles and shedding light on the dynamic nature of the microbial communities in soil. This review focuses on the principle of metagenomics techniques, their potential applications and limitations in soil microbial diversity analysis. The effectiveness of target-based metagenomics in determining the function of individual genes and microorganisms in soil ecosystems is also highlighted. Targeted metagenomics, including high-throughput sequencing and stable-isotope probing, is essential for studying microbial taxa and genes in complex ecosystems. Shotgun metagenomics may reveal the diversity of soil bacteria, composition, and function impacted by land use and soil management. Sanger, Next Generation Sequencing, Illumina, and Ion Torrent sequencing revolutionise soil microbiome research. Oxford Nanopore Technology (ONT) and Pacific Biosciences (PacBio)'s third and fourth generation sequencing systems revolutionise long-read technology. GeoChip, clone libraries, metagenomics, and metabarcoding help comprehend soil microbial communities. The article indicates that metagenomics may improve environmental management and agriculture despite existing limitations.Metagenomics has revolutionised soil microbiology research by revealing the complete diversity, function, and interactions of microorganisms in soil. Metagenomics is anticipated to continue defining the future of soil microbiology research despite some limitations, such as the difficulty of locating the appropriate sequencing method for specific genes.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100226"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000087/pdfft?md5=2ce9d5e7893eae4a50ce2b1e5b444897&pid=1-s2.0-S2666517424000087-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139942448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical profiling of fungal metabolites via the OSMAC approach: Novel identification of Brianthein W from an endophytic fungus, Hypomontagnella monticulosa Zg15SU","authors":"Anisa Lutfia ,&nbsp;Erman Munir ,&nbsp;Yurnaliza Yurnaliza ,&nbsp;Mohammad Basyuni ,&nbsp;Hirosuke Oku","doi":"10.1016/j.crmicr.2024.100288","DOIUrl":"10.1016/j.crmicr.2024.100288","url":null,"abstract":"<div><div>In the previous report, we reported that <em>Hypomontagnella monticulosa</em> originating from the rhizome of <em>Zingiber griffithii</em> was known to produce a marine-derived natural product. An OSMAC-based approach was designed by modifying the nutritional composition of the growth medium to investigate any possible new metabolites produced by the strain. The culture filtrate and biomass were conditioned through the use of three basal media, namely, Czapek-dox, potato dextrose, and Wickerham broth medium. GC–MS and multivariate analysis was performed to distinguish the chemicals and determine their composition in the tested extracts. Antimicrobial activity was tested against selected human pathogenic microbes using the disk-diffusion method. The MeOH extract of both culture filtrate and biomass from different fermentation media revealed that the majority of identified compounds (<em>n</em> = 40) were found in Wickerham medium (<em>n</em> = 23), which is later termed as MeOH-Wi. The chemical composition of MeOH-Wi was fatty acids (21.74 %), followed by terpenoids (17.38 %), cyclosiloxane (13.04 %), aldehydes (13.04 %), alkenes (8.7 %), hydrocarbons (8.7 %), esters (8.7 %), alkaloids (4.35 %), and an unclassified compound, the 9,9-Dimethyl-3,7-diazabicyclo[3.3.1]nonane. The numerous chemical compounds in MeOH-Wi corresponded with its antimicrobial activities against <em>Micrococcus luteus</em> NBRC 13,867 (±11 mm), <em>Candida maltosa</em> NBRC 1977 (±9 mm), and <em>Escherichia coli</em> JM 109 (±5 mm) which were higher than the other tested extracts. Further purification using HPLC RP-C₁₈ using NP-SiO₂/<em>n</em>-Hex–EtOAc (10:2) to yield compound <strong>(1)</strong>. Compound <strong>(1)</strong> was determined as an analog of briarian W—a diterpene mostly found in marine sponge, <em>Briareum</em> spp.—as a pure compound in the MeOH extract of <em>H. monticulosa</em> based on the 1D and 2D NMR analysis. The anticancer activity (IC₅₀) of compound <strong>(1)</strong> was 0.077, 0.080, and 0.102 µg/mL against the growth of HCT-116, NBT-T2, and Panc-1, respectively. As far as we are aware, this is the first report on finding a briarian diterpene that originates from an endophytic fungus, especially by <em>H. monticulosa.</em></div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100288"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiseptics: An expeditious third force in the prevention and management of coronavirus diseases","authors":"Kizito I. Okeke ,&nbsp;Chukwuemeka Samson Ahamefule ,&nbsp;Obianuju O. Nnabuife ,&nbsp;Ibuchukwu N. Orabueze ,&nbsp;Christian U. Iroegbu ,&nbsp;Kingsley A. Egbe ,&nbsp;Anthony C. Ike","doi":"10.1016/j.crmicr.2024.100293","DOIUrl":"10.1016/j.crmicr.2024.100293","url":null,"abstract":"<div><div>Notably, severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS) and coronavirus disease 2019 (COVID-19) have all had significant negative impact on global health and economy. COVID-19 alone, has resulted to millions of deaths with new cases and mortality still being reported in its various waves. The development and use of vaccines have not stopped the transmission of SARS coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, even among vaccinated individuals. The use of vaccines and curative drugs should be supplemented with adoption of simple hygiene preventive measures in the fight against the spread of the virus, especially for healthcare workers. Several virucidal topical antiseptics, such as povidone-iodine (PVP-I), citrox, cyclodextrins among others, have been demonstrated to be efficacious in the inactivation of SARS-CoV-2 and other coronaviruses in both <em>in vitro</em> and <em>in vivo</em> studies. The strategic application of these virucidal formulations could provide the additional impetus needed to effectively control the spread of the virus. We have here presented a simple dimension towards curtailing the dissemination of COVID-19, and other coronaviruses, through the application of effective oral, nasal and eye antiseptics among patients and medical personnel. We have further discussed the mechanism of action of some of these commonly available virucidal solutions while also highlighting some essential controversies in their use.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100293"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Host-Parasite interaction between brown algae and eukaryote biotrophic pathogens","authors":"Anagha Santhosh,&nbsp;Sigrid Neuhauser","doi":"10.1016/j.crmicr.2024.100306","DOIUrl":"10.1016/j.crmicr.2024.100306","url":null,"abstract":"<div><div>Brown algae belong to the class Phaeophyceae which are mainly multicellular, photosynthetic organisms, however they evolved independently from terrestrial plants, green and red algae. In the past years marine aquaculture involving brown algae has gained enormous momentum. In both natural environments and aquaculture, brown algae are susceptible to infection by various prokaryotic and eukaryotic parasites. While our understanding of host-parasite interactions in brown algae is gaining recognition, our understanding of how brown algae react to biotic stress remains incomplete. The objective of this review is to address research gaps in the field by providing a summary of what is already known about the response of brown algae to abiotic and biotic stress. The biology of eukaryotic zoosporic pathogens <em>Maullinia ectocarpii, Eurychasma dicksonii, Anisolpidium ectocarpii</em> is also discussed, as those parasites have been used in laboratory experiments to study diseases of brown algae. These studies often relied on parasites-infecting <em>Ectocarpus siliculosus</em> which has become a brown algal model organism to study host-pathogen interactions. Stress response in brown algae involves processes similar to hypersensitivity response, oxidative stress response, and activation of peroxidases, but also the production of blue fluorescent metabolites and deposition of β-1,3-glucan in the cell wall. Cell wall modification, expression of several defence related proteins, and secondary metabolite production also hold a crucial role in brown algal defence mechanism. Understanding host-pathogen interactions and the associated mechanisms is vital to discover strategies to control pathogens in the growing aquaculture sector.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100306"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142593268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Major facilitator superfamily efflux pumps in human pathogens: Role in multidrug resistance and beyond","authors":"Manjusha Lekshmi ,&nbsp;Anely Ortiz-Alegria ,&nbsp;Sanath Kumar ,&nbsp;Manuel F. Varela","doi":"10.1016/j.crmicr.2024.100248","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100248","url":null,"abstract":"<div><p>The major facilitator superfamily (MFS) of proteins constitutes a large group of related solute transporters found across all known living taxa of organisms. The transporters of the MFS contain an extremely diverse array of substrates, including ions, molecules of intermediary metabolism, and structurally different antimicrobial agents. First discovered over 30 years ago, the MFS represents an important collection of integral membrane transporters. Bacterial microorganisms expressing multidrug efflux pumps belonging to the MFS are considered serious pathogens, accounting for alarming morbidity and mortality numbers annually. This review article considers recent advances in the structure-function relationships, the transport mechanism, and modulation of MFS multidrug efflux pumps within the context of drug resistance mechanisms of bacterial pathogens of public health concerns.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100248"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000300/pdfft?md5=47b1021afa94b863c07c4c68242304cf&pid=1-s2.0-S2666517424000300-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141324862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}