Current Research in Microbial Sciences最新文献_第6页

Changes in ppGpp levels impact gene expression and virulence features of Adherent-Invasive Escherichia coli strain LF82 ppGpp水平的变化影响粘附-侵袭型大肠杆菌LF82的基因表达和毒力特征。

IF 5.6

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-07-07 DOI: 10.1016/j.crmicr.2026.100641

Llorenç Fernández-Coll, Queralt Bonet-Rossinyol, Mireia López-Siles, Katarzyna Potrykus, Maria Núria Ramos-Corominas, Margarita Martinez-Medina

{"title":"Changes in ppGpp levels impact gene expression and virulence features of Adherent-Invasive Escherichia coli strain LF82","authors":"Llorenç Fernández-Coll, Queralt Bonet-Rossinyol, Mireia López-Siles, Katarzyna Potrykus, Maria Núria Ramos-Corominas, Margarita Martinez-Medina","doi":"10.1016/j.crmicr.2026.100641","DOIUrl":"10.1016/j.crmicr.2026.100641","url":null,"abstract":"<div><div>Strain LF82 belongs to the Adherent-Invasive <em>Escherichia coli</em> (AIEC) pathotype, found in 21% to 63% of Crohn’s disease (CD) patients. Many studies have explored its specific virulence mechanisms, but AIEC isolates are genomically similar to non-invasive isolates of <em>E. coli</em>, suggesting a differential gene expression. Here we study the impact of the global regulator ppGpp in the expression of AIEC’s virulence factors and its potential for therapeutic and diagnostic purposes. Levels of ppGpp were determined from P-33 labelled cultures of LF82 and MG1655 (commensal strain) and differences in gene expression during infection of intestinal epithelial cells were assessed through RNAseq. Our study revealed that ppGpp is responsible for the expression of different genes involved in AIEC’s pathogenicity (Type I fimbriae, T6SS or Vat), and other global regulators (as CRP or c-di-GMP). AIEC characteristics and virulence features (biofilm formation and motility) were determined, showing that ppGpp is essential for LF82 to adhere to and invade epithelial intestinal cells, as well as to survive in macrophages. Lastly, virulence was evaluated <em>in vivo</em> using the <em>Galleria mellonella</em> larvae model, showing a decrease in virulence in the absence of ppGpp. Analysis of a panel of differentially expressed genes across a collection of 7 AIEC strains and 6 non-AIEC strains, identified two candidate biomarkers which expression show a discriminatory potential for AIEC identification in the future. Altogether, our findings establish ppGpp as a key regulator of the AIEC virulence program and provide novel targets for both therapeutic intervention and diagnostic development.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100641"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13356692/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148430927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics and longitudinal stability of Gut Microbiota in healthy individuals across different age groups 不同年龄组健康个体肠道菌群的特征和纵向稳定性

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2025-11-20 DOI: 10.1016/j.crmicr.2025.100512

Biao Zou , Qian’er Huo , Xianfeng Zhou , Yongling Lv , Guolong Li , Guang Fu , Hexiao Shen , Sainan Shu

{"title":"Characteristics and longitudinal stability of Gut Microbiota in healthy individuals across different age groups","authors":"Biao Zou , Qian’er Huo , Xianfeng Zhou , Yongling Lv , Guolong Li , Guang Fu , Hexiao Shen , Sainan Shu","doi":"10.1016/j.crmicr.2025.100512","DOIUrl":"10.1016/j.crmicr.2025.100512","url":null,"abstract":"<div><div>Fecal microbiota transplantation (FMT) efficacy relies on donor microbiome composition and temporal stability, yet the influence of donor age remains inadequately investigated. This longitudinal analysis addressed this gap by examining 81 healthy individuals (3–30 years), stratified into four age groups, who provided monthly fecal samples over 12 months (<em>n</em> = 972 samples). Gut microbiota composition (16S rDNA sequencing) and temporal stability were assessed using Bray–Curtis dissimilarity, intraclass correlation coefficient (ICC), and genus-level co-occurrence network analysis. Results demonstrated a strong age-dependency in microbiota stability. The teenage cohort (13–17 years) exhibited the highest stability, characterized by minimal fluctuations in α- and β-diversity and significantly stronger network centrality. Furthermore, specific genera, notably <em>Faecalibacterium</em> and <em>Bifidobacterium</em>, displayed exceptionally high ICC values (>0.90), identifying them as core taxa associated with temporal consistency. These findings underscore the critical role of donor age in microbial stability and highlight teenagers as possessing optimal microbiota characteristics for FMT. They strongly support the development of an ICC-based screening framework to enhance donor selection protocols.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100512"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145645848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fimbriae potentiate Aggregatibacter actinomycetemcomitans for periodontal disease 菌毛增强聚集菌放线菌对牙周病的影响

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-02-01 DOI: 10.1016/j.crmicr.2026.100566

Yanyan Fu, Qiqi Pan, Jan Maarten van Dijl

{"title":"Fimbriae potentiate Aggregatibacter actinomycetemcomitans for periodontal disease","authors":"Yanyan Fu, Qiqi Pan, Jan Maarten van Dijl","doi":"10.1016/j.crmicr.2026.100566","DOIUrl":"10.1016/j.crmicr.2026.100566","url":null,"abstract":"<div><div><em>Aggregatibacter actinomycetemcomitans</em> is a major contributor to aggressive periodontitis and has been increasingly implicated in systemic conditions, such as rheumatoid arthritis and Alzheimer’s disease. Among its diverse virulence mechanisms, fimbriae have emerged as central factors driving colonization, immune evasion, and pathogenicity. This review highlights recent findings showing that fimbriae not only facilitate adhesion and biofilm formation, but also contribute to maintaining bacterial cell envelope integrity. The transition from rough (fimbriated) to smooth (non-fimbriated) colony phenotypes upon <em>in vitro</em> culturing is associated with significant changes in virulence, including increased release of outer membrane vesicles (OMVs) and extracellular cytoplasmic proteins (ECPs). These changes suggest a trade-off between surface structure expression and secretion-based virulence. Importantly, fimbriae-rich strains are clinically relevant and more virulent in host models. The combined evidence implies that fimbriae and key virulence factors, such as leukotoxin A, act cooperatively in pathogenesis, as fimbriae-mediated adhesion and persistence will enhance local toxin delivery, killing of immune cells and immune evasion. Together, these insights point to fimbriae as key targets for novel therapeutic and vaccination strategies aimed at controlling periodontal and related systemic diseases.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100566"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated metagenomic and metabolomic analysis reveals regional style differences in Maotai-flavour Baijiu 综合元基因组和代谢组学分析揭示了茅台味白酒的地域风格差异

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-02-03 DOI: 10.1016/j.crmicr.2026.100558

Xin Shi , Chenming Fan , Ming Hui , Qing Tian , Fangli Zhang , Chunmei Pan

{"title":"Integrated metagenomic and metabolomic analysis reveals regional style differences in Maotai-flavour Baijiu","authors":"Xin Shi , Chenming Fan , Ming Hui , Qing Tian , Fangli Zhang , Chunmei Pan","doi":"10.1016/j.crmicr.2026.100558","DOIUrl":"10.1016/j.crmicr.2026.100558","url":null,"abstract":"<div><div>This research focused on Maotai-flavour Baijiu from three representative production regions in the Chishui River Basin, namely Maotai Town (MT), Jinsha (JS), and Renhuai (RH). By integrating metabolomics and macrogenomics techniques, the study analyzed the differences in volatile flavor compounds and microbial community structures in the fourth-round fermented grains and base liquor. Additionally, it explored the associative mechanism between microorganisms and flavor metabolism. The findings indicate that the microbial community compositions of fermented grains vary significantly across different production areas. The production area of Maotai Town mainly consists of <em>Saccharomyces</em> and <em>Lactobacillus</em>, with the highest content of ester substances. The fungal community in Jinsha is extremely stochastic, abundant in Mucoromycota, and has elevated contents of aldehydes and phenols. The distribution of microorganisms and flavor substances in the Renhuai production area lies between the two. The sensory evaluation of the base liquor indicates that the Jinsha production area features prominent floral and fruity aromas, the Maotai Town exhibits significant sour and sauce aromas, and the Renhuai production area has a well-balanced flavor. The correlation analysis shows that yeasts such as <em>Saccharomyces</em> and <em>Pichia</em> are positively correlated with esters such as ethyl acetate, while bacteria such as <em>Limosilactobacillus</em> are closely associated with short-chain fatty acid metabolism. This research reveals the microbiological basis for the differences in the Maotai style among different production regions and provides a theoretical foundation for regional characteristic production and process optimization.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100558"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A naturally isolated symbiotic Lactobacillus murinus suppresses multidrug-resistant Campylobacter jejuni via microbial metabolites 天然分离的共生鼠乳杆菌通过微生物代谢物抑制多重耐药空肠弯曲杆菌

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2025-11-25 DOI: 10.1016/j.crmicr.2025.100520

Junhao Wang , Ali Asif , Fangtong Gu , Shuaifeng Gu , Yakun Ding , Yufeng Gu , Muhammad Shahzad Rafiq , Haihong Hao

{"title":"A naturally isolated symbiotic Lactobacillus murinus suppresses multidrug-resistant Campylobacter jejuni via microbial metabolites","authors":"Junhao Wang , Ali Asif , Fangtong Gu , Shuaifeng Gu , Yakun Ding , Yufeng Gu , Muhammad Shahzad Rafiq , Haihong Hao","doi":"10.1016/j.crmicr.2025.100520","DOIUrl":"10.1016/j.crmicr.2025.100520","url":null,"abstract":"<div><div>Multidrug-resistant <em>Campylobacter jejuni</em> (MDR <em>C. jejuni</em>), the leading cause of food-borne gastroenteritis worldwide, poses a significant threat to public health and food safety. The intestinal microbiota prevents MDR <em>C. jejuni</em> colonization, but the specific mechanisms remain poorly understood. In this study, we performed a multi-omics analysis of the gut microbiota in C57BL/6 mice, combined with <em>in vitro</em> experiments, to investigate the role of gut microbiota in <em>C. jejuni</em> colonization. Treatment with tylvalosin, a new macrolide, altered the gut microbiota composition, reducing <em>Bifidobacterium longum</em> communities and decreasing levels of short-chain fatty acids (acetic acid, propionic acid, <em>n</em>-butyric acid, <em>i</em>-butyric acid, and <em>i</em>-valeric acid). This disruption of intestinal homeostasis facilitated <em>C. jejuni</em> colonization. Through metagenomic sequencing, we identified and isolated <em>Lactobacillus murinus</em> (<em>L. murinus</em>) from the mice’s intestinal flora, which exhibited inhibitory activity against <em>C. jejuni in vitro</em>. Metabolomic analysis and <em>in vitro</em> validation further revealed the significance of <em>L. murinus</em>-derived metabolites. Our results indicate that <em>L. murinus</em> inhibits and kills <em>C. jejuni</em> in a co-culture system by secreting acids that synergistically induce apoptosis, leading to cell membrane disruption and the release of cellular contents.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100520"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145683974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uncovering differences in rye and wheat degradation by human gut microbiota applying a quantitative multi-metaOmics in vitro approach 应用体外多元组学方法揭示人肠道微生物群对黑麦和小麦降解的差异

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2025-12-09 DOI: 10.1016/j.crmicr.2025.100532

Clara Berenike Arnold , Alexandra Kelder , Sabrina Woltemate , Robert Geffers , Andreas von Felde , Knud Erik Bach Knudsen , Christian Visscher , Marius Vital

{"title":"Uncovering differences in rye and wheat degradation by human gut microbiota applying a quantitative multi-metaOmics in vitro approach","authors":"Clara Berenike Arnold , Alexandra Kelder , Sabrina Woltemate , Robert Geffers , Andreas von Felde , Knud Erik Bach Knudsen , Christian Visscher , Marius Vital","doi":"10.1016/j.crmicr.2025.100532","DOIUrl":"10.1016/j.crmicr.2025.100532","url":null,"abstract":"<div><div>While wheat is the most common grain used in bread-making worldwide, rye is popular in many European countries too. Rye is associated with several health benefits, which is attributed to its comparatively higher dietary fiber content (primarily fructans and arabinoxylans) that promote production of short chain fatty acids (SCFA) by gut microbiota, in particular butyrate. Intervention studies revealed bacterial alterations upon rye administration, however, the detailed mechanisms involved in its degradation are not understood.</div><div>We grew fecal communities (<em>n</em> = 20) on pre-digested rye and wheat, respectively, demonstrating that rye was yielding higher cell and SCFA concentrations in almost all samples along with distinct abundances of many taxa. A multi metaOmics (metagenomics/metatranscriptomics) approach (<em>n</em> = 5 donors) showed higher bacterial growth rates for most taxa on rye compared to wheat. The higher growth rate of rye was accompanied by increased expression of genes involved in growth and energy generation suggesting higher carbon substrate accessibility. The carbohydrate active enzyme repertoire was greatly distinct between communities growing on the two substrates with several specific glycoside hydrolases increasingly expressed in rye containing cultures. <em>Agathobacter faecis</em> was revealed as the key butyrogenic species for rye degradation and its expression pattern based on metagenome assembled genomes showed adaptation to growth on rye via expression of genes involved in arabinoxylan degradation and fructose (major monomer of fructans) uptake.</div><div>Our study verifies higher SCFA production from rye over wheat and gives detailed insights into molecular mechanisms involved. It suggests that the observed health benefits of rye are mediated by gut microbiota.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100532"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shared signatures of alcohol-associated dysbiosis in humans and non-human primates 人类和非人类灵长类动物酒精相关生态失调的共同特征

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2025-12-14 DOI: 10.1016/j.crmicr.2025.100534

Ti Chen , Rui Huang , Yichun Huang , Jianhong Wang , Zheng Wang , Xiaojie Zhang

{"title":"Shared signatures of alcohol-associated dysbiosis in humans and non-human primates","authors":"Ti Chen , Rui Huang , Yichun Huang , Jianhong Wang , Zheng Wang , Xiaojie Zhang","doi":"10.1016/j.crmicr.2025.100534","DOIUrl":"10.1016/j.crmicr.2025.100534","url":null,"abstract":"<div><div>Alcohol use disorder (AUD) is a chronic brain disease with limited therapeutic options. Increasing evidence suggests that the gut microbiome contributes to AUD via the microbiome–gut–brain axis. Here, we conducted a cross-species investigation of gut microbiota alterations in patients with clinically diagnosed AUD and in non-human primates (NHPs) subjected to long-term alcohol (ethanol) self-administration, using metagenomic sequencing. Both cohorts showed reduced microbial diversity and conserved dysbiosis, with consistent depletion of <em>Verrucomicrobia, Actinobacteria, Faecalibacterium, Akkermansia, Intestinibacter, Phascolarctobacterium</em>, and <em>Ruminococcus</em>, alongside increased <em>Blautia</em> and <em>Coprococcus</em>. These microbial shifts correlated with liver function indices, notably positive associations between <em>Ruminococcus</em> and bilirubin levels in both species, suggesting a potential role in liver injury. Functional analyses revealed conserved microbial adaptations, including upregulated DNA repair pathways, fermentative energy metabolism, and downregulated glutamate/glutamine biosynthesis. Together, these results identify evolutionarily conserved microbial and metabolic alterations linking alcohol consumption, gut dysbiosis, and hepatic dysfunction. Our cross-species evidence highlights the gut microbiome as a potential biomarker and therapeutic target for AUD.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100534"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-glycoprotein synergized recombinant virus system resolved the research dilemma of Epstein-Barr virus entry and facilitated the development of entry-specific drugs or vaccines 多糖蛋白协同重组病毒系统解决了Epstein-Barr病毒进入的研究困境，促进了进入特异性药物或疫苗的开发。

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-02-21 DOI: 10.1016/j.crmicr.2026.100572

Tianyu Lu , Shufeng Tian , Jiapeng Luo , Zhe Zhou , Yanying Liang , Wei Xu , Shenglei Yu , Lingyun Shao , Huang Huang , Lu Lu , Jikui Deng , Jing Wu , Wenhong Zhang

{"title":"A multi-glycoprotein synergized recombinant virus system resolved the research dilemma of Epstein-Barr virus entry and facilitated the development of entry-specific drugs or vaccines","authors":"Tianyu Lu , Shufeng Tian , Jiapeng Luo , Zhe Zhou , Yanying Liang , Wei Xu , Shenglei Yu , Lingyun Shao , Huang Huang , Lu Lu , Jikui Deng , Jing Wu , Wenhong Zhang","doi":"10.1016/j.crmicr.2026.100572","DOIUrl":"10.1016/j.crmicr.2026.100572","url":null,"abstract":"<div><div>Epstein-Barr virus (EBV) causes multiple severe diseases, and its entry process is a promising prophylactic and therapeutic target. However, EBV entry is intricate with several unresolved mechanisms, and current research methods for it is limited, insensitive, and inefficient. Therefore, novels methods are urgently needed. Herein, we innovatively reported a recombinant virus system rEBV-VSV realizing complicated multi-glycoprotein synergized EBV entry with sensitive detection, which was especially suitable for accurate study of entry mechanisms and effective evaluation of entry-inhibitive therapies. First, rEBV-VSV efficiently entered host cells via multi-synergetic EBV glycoproteins on its membrane and simulated entry features and cellular tropisms of native EBV, also being sensitively detected via bioluminescence. Second, rEBV-VSV was an effective measuring system to evaluate entry-inhibiting potency of EBV-specific antibodies and population’s sera in a rapid and high-throughput manner. Third, via flexible mutations and recombination on rEBV-VSV, it was used to accurately study functions of glycoproteins and key residues during EBV entry to better understand entry mechanisms. Especially, via rEBV-VSV, glycoprotein H (gH) mutation L65A+L69A was found to have completely opposite effects on EBV entry into B and epithelia cells, which was manipulated by gp42, and gp42 also powerfully impacted the efficacy of gH-specific antibody E1D1. Conclusively, rEBV-VSV was a satisfactory system to study EBV entry features, mechanisms, and entry-targeted therapies, which could resolve the research dilemma of EBV entry and facilitate the development of EBV-specific drugs and vaccines.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100572"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147378788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

C6‑HSL enhances BPA tolerance in Pseudomonas asiatica: an integrative Raman‑DIP and transcriptomic study C6 - HSL增强亚洲假单胞菌对BPA的耐受性：一项综合拉曼- DIP和转录组学研究

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2025-12-05 DOI: 10.1016/j.crmicr.2025.100530

Kejian Tian , Fenglin Zhang , Yibing Wang , Jinming Gu , Dandan Zhou , Fanxing Meng , Wenkang Mu , Pengfei Hao , Hongliang Huo , Fan Li

{"title":"C6‑HSL enhances BPA tolerance in Pseudomonas asiatica: an integrative Raman‑DIP and transcriptomic study","authors":"Kejian Tian , Fenglin Zhang , Yibing Wang , Jinming Gu , Dandan Zhou , Fanxing Meng , Wenkang Mu , Pengfei Hao , Hongliang Huo , Fan Li","doi":"10.1016/j.crmicr.2025.100530","DOIUrl":"10.1016/j.crmicr.2025.100530","url":null,"abstract":"<div><div>Bisphenol A (BPA) is a pervasive contaminant found in various environmental media, exhibiting extensive biotoxicity to microorganisms. Consequently, it would be of paramount significance to investigate broader methods and mechanisms for enhancing bacterial resistance to stress. This study found that BPA stress can lead to the downregulation of over 59 % of functional genes and result in a maximum reduction of cell viability and ATP content in the strain. Conversely, 10 μM C6-HSL has been observed to enhance the strain's resistance to BPA toxicity stress most significantly by inducing up-regulation of genes of the pathways of antioxidant damage, amino acid synthesis and metabolism, and energy supply, and increased the cell viability by 1.13-fold. The up-regulated expression of antioxidant stress genes directly enhance the bacterial resistance to BPA toxicity, resulting in a 17.28 % reduction in intracellular ROS. On this basis, C6-HSL could increase the expression levels of genes related to amino acid and energy synthesis, as well as enzyme activities. This promotes intracellular metabolic processes and energy production, ultimately elevating the ATP content within the strain by 12.21 %. This study is the first to confirm the function of C6-HSL in enhancing bacterial resistance to BPA toxicity and innovatively combines Raman-DIP, transcriptomics, and enzyme activity to systematically analyse the regulatory mechanisms of C6-HSL on the bacterial antioxidant system, amino acid system, and energy system. The study provides methodological references and theoretical foundations for comprehensively enhancing the detoxification ability of microorganisms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100530"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145737804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Methods to characterize the vaginal microbiome in a rhesus macaque model of simian human immunodeficiency virus (SHIV) transmission uncover epithelium-associated enrichment of Prevotella 在猴人免疫缺陷病毒（SHIV）传播的恒河猴模型中，对阴道微生物组进行表征的方法揭示了普雷沃氏菌的上皮相关富集

IF 5.8

Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2025-12-03 DOI: 10.1016/j.crmicr.2025.100526

Rahul Mohan , Samuel D. Johnson , Paden N. Dean, Arpan Acharya, Siddappa N. Byrareddy

{"title":"Methods to characterize the vaginal microbiome in a rhesus macaque model of simian human immunodeficiency virus (SHIV) transmission uncover epithelium-associated enrichment of Prevotella","authors":"Rahul Mohan , Samuel D. Johnson , Paden N. Dean, Arpan Acharya, Siddappa N. Byrareddy","doi":"10.1016/j.crmicr.2025.100526","DOIUrl":"10.1016/j.crmicr.2025.100526","url":null,"abstract":"<div><div>The vaginal microbiome plays a crucial role in maintaining mucosal integrity and mitigating pathogen transmission, yet its comprehensive characterization remains challenging due to limited sampling and analysis methods. In this study, we aimed to characterize bacterial and fungal taxa diversities in the vaginal microbiomes of Simian Human Immunodeficiency (SHIV)-infected rhesus macaques, as well as their metabolic activities, using three sampling methods. The cervicovaginal lavage (CVL), vaginal swab, and vaginal mucosal tissue methods offer novel insights into microbial diversity and their potential impacts on HIV transmission. Using 16S rRNA and Internal Transcribed Spacer (ITS) sequencing, we assessed bacterial and fungal community composition and abundances, respectively, across all sampling methods. PICRUSt2 was used for functional predictions, and a modified glycosidase assay to further characterize glycan-degrading enzymatic activity in CVL samples. Our findings reveal that tissue samples were uniquely enriched for microbial taxa such as <em>Prevotella</em> spp. and <em>Helicobacter</em> spp., showing notable abundance differences compared to CVL and swab samples. Tissue samples exhibited higher alpha diversity and distinct metabolic prediction profiles, particularly elevated sialidase activity. While fewer differences were found in fungal microbiome composition and diversity, marked correlations were observed between bacterial and fungal taxa, emphasizing complex interkingdom interactions. These results highlight the significance of sampling methods in microbial ecology studies, which should be carefully considered due to their potential influence on pathogen transmission risk.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100526"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145790538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0