Current Research in Microbial Sciences最新文献

{"title":"The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in <i>Acinetobacter baumannii</i>.","authors":"Sirui Zhang, Jingdan Wang, Rong Yu, Haiping Liu, Shuyan Liu, Kai Luo, Jin'e Lei, Bei Han, Yanjiong Chen, Shaoshan Han, E Yang, Meng Xun, Lei Han","doi":"10.1016/j.crmicr.2024.100332","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100332","url":null,"abstract":"<p><p>Although various mechanisms of carbapenem-resistance have been identified in the nosocomial pathogen <i>Acinetobacter baumannii</i>, the critical process of resistance evolution and the factors involved in are not well understood. Herein, we identified a universal stress protein Usp1413 which played an important role in adaptive resistance of <i>A. baumannii</i> to meropenem (MEM). Based on RNA-Seq and genome sequencing, Usp1413 was not only one of the most downregulated USPs, but also the bare one having mutation of tyrosine and glycine inserted at the site of 229-230 (YG229-230) under the stimulation of MEM. Deletion of Usp1413 resulted in increased MEM resistance. In addition, Usp1413 affected the bacterial abilities of biofilm formation and swarm motility, as well as helped <i>A. baumannii</i> response to various environmental stresses. These effects of Usp1413 were achieved by regulating its interaction proteins, within the functions of YigZ family protein, acetyltransferase, and SulP family inorganic anion transporter. The insertion mutation of YG229-230 influenced both the expression of interaction proteins and the phenotypes of bacteria. Finally, the promotor region of Usp1413 was convinced by point mutations. Overall, our findings identified the universal stress protein Usp1413 as a contributor involved in MEM adaptive resistance and responded to numerous environmental stresses. This study provides novel insights into the mechanism of universal stress proteins in participating antibiotic resistance, and affords a potential target for controlling drug resistance development in <i>A. baumannii</i>.</p>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"100332"},"PeriodicalIF":4.8,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human microbiome in post-acute COVID-19 syndrome (PACS).","authors":"Arezoo Fallah, Hamid Sedighian, Reza Kachuei, Abbas Ali Imani Fooladi","doi":"10.1016/j.crmicr.2024.100324","DOIUrl":"10.1016/j.crmicr.2024.100324","url":null,"abstract":"<p><p>The global COVID-19 pandemic, which began in 2019, is still ongoing. SARS-CoV-2, also known as the severe acute respiratory syndrome coronavirus 2, is the causative agent. Diarrhea, nausea, and vomiting are common GI symptoms observed in a significant number of COVID-19 patients. Additionally, the respiratory and GI tracts express high level of transmembrane protease serine 2 (TMPRSS2) and angiotensin-converting enzyme-2 (ACE2), making them primary sites for human microbiota and targets for SARS-CoV-2 infection. A growing body of research indicates that individuals with COVID-19 and post-acute COVID-19 syndrome (PACS) exhibit considerable alterations in their microbiome. In various human disorders, including diabetes, obesity, cancer, ulcerative colitis, Crohn's disease, and several viral infections, the microbiota play a significant immunomodulatory role. In this review, we investigate the potential therapeutic implications of the interactions between host microbiota and COVID-19. Microbiota-derived metabolites and components serve as primary mediators of microbiota-host interactions, influencing host immunity. We discuss the various mechanisms through which these metabolites or components produced by the microbiota impact the host's immune response to SARS-CoV-2 infection. Additionally, we address confounding factors in microbiome studies. Finally, we examine and discuss about a range of potential microbiota-based prophylactic measures and treatments for COVID-19 and PACS, as well as their effects on clinical outcomes and disease severity.</p>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"100324"},"PeriodicalIF":4.8,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorus dynamics and sustainable agriculture: The role of microbial solubilization and innovations in nutrient management","authors":"José Abraham García-Berumen ,&nbsp;Juan Armando Flores de la Torre ,&nbsp;Sergio de los Santos-Villalobos ,&nbsp;Alejandro Espinoza-Canales ,&nbsp;Francisco Guadalupe Echavarría-Cháirez ,&nbsp;Héctor Gutiérrez-Bañuelos","doi":"10.1016/j.crmicr.2024.100326","DOIUrl":"10.1016/j.crmicr.2024.100326","url":null,"abstract":"<div><div>Phosphorus (P) is an essential element for plant growth, playing a crucial role in various metabolic processes. Despite its importance, phosphorus availability in soils is often restricted due to its tendency to form insoluble complexes, limiting plant uptake. The increasing demand for phosphorus in agriculture, combined with limited global reserves of phosphate rock, has created challenges for sustainable plant production. Additionally, the overuse of chemical phosphorus fertilizers has resulted in environmental degradation, such as eutrophication of water bodies. Increasing agronomic phosphorus (P) efficiency is crucial because of population growth and increased food demand. Hence, microorganisms involved in the P cycle are a promising biotechnological strategy that has gained global interest in recent decades. Microorganisms' solubilization of phosphate rock (PR) is an environmentally sustainable alternative to chemical processing for producing phosphate fertilizers. Phosphorus-solubilizing microorganisms (PSMs), including bacteria and fungi, and their enzymatic processes offer an eco-friendly and sustainable alternative to chemical inputs by converting insoluble phosphorus into forms readily available for plant uptake. Integrating PSMs into agricultural systems presents a promising strategy to reduce dependence on chemical fertilizers, enhance soil health, and contribute to the transition toward more sustainable and resilient agricultural practices. It can be an alternative that reduces the loss of phosphorus in the environment, especially the eutrophication of aquatic systems. This paper explores the challenges of phosphorus availability in agriculture and the potential of microbial phosphorus solubilization as a sustainable alternative to conventional practices.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100326"},"PeriodicalIF":4.8,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering latent infections in kidneys: A novel molecular approach for differential Leptospira detection","authors":"Li-Fang Chou ,&nbsp;Yi-Chun Liu ,&nbsp;Huang-Yu Yang ,&nbsp;Ya-Chung Tian ,&nbsp;Chih-Ho Lai ,&nbsp;Ming-Yang Chang ,&nbsp;Cheng-Chieh Hung ,&nbsp;Tong-Hong Wang ,&nbsp;Shen-Hsing Hsu ,&nbsp;Chung-Ying Tsai ,&nbsp;Pei-Yu Hung ,&nbsp;Chih-Wei Yang","doi":"10.1016/j.crmicr.2024.100327","DOIUrl":"10.1016/j.crmicr.2024.100327","url":null,"abstract":"<div><div>Leptospirosis, a re-emerging zoonotic disease caused by <em>Leptospira</em> spp., poses significant global health and veterinary challenges. Long-term colonization of renal tubules by <em>Leptospira</em> in asymptomatic hosts highlights the need for sensitive detection methods. This study evaluates the chronic or latent <em>Leptospira</em> infections in kidneys using a novel molecular approach to examine individual immune responses differences. Digital PCR strategies employing newly developed primer-probe sets targeting the flagellar <em>fliG</em> gene were used to assess the presence of trace <em>Leptospira</em> in infected murine kidneys and urine samples from laboratory-confirmed leptospirosis patients. RNA-based digital PCR detected leptospires in 58 % (targeting <em>lipl32</em>) and 83 % (targeting <em>fliG</em>) of infected kidneys, demonstrating that the digital PCR strategy targeting the <em>fliG</em> gene offers superior sensitivity. Notably, the newly developed <em>fliG</em>-targeting assay detected as low as 20 fg of <em>Leptospira</em> DNA, offering ten-fold greater sensitivity than traditional qPCR for trace detection. This allows for differential detection of <em>Leptospira</em> species and facilitates monitoring of extremely low bacterial loads with greater sensitivity than conventional methods. We also observed regenerating renal tubules with mitosis and elevated cytokine expression in kidneys with transcriptionally active <em>Leptospira</em> during chronic infection. This approach aids in identifying latent infections and offers insights into individual variations. Our research provides a powerful molecular tool for epidemiological studies and public health surveillance, contributing valuable insights into the prevalence and transmission dynamics of this pervasive zoonotic disease.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100327"},"PeriodicalIF":4.8,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rhizosphere microbiome regulation: Unlocking the potential for plant growth","authors":"Chenghua Luo ,&nbsp;Yijun He ,&nbsp;Yaping Chen","doi":"10.1016/j.crmicr.2024.100322","DOIUrl":"10.1016/j.crmicr.2024.100322","url":null,"abstract":"<div><div>Rhizosphere microbial communities are essential for plant growth and health maintenance, but their recruitment and functions are affected by their interactions with host plants. Finding ways to use the interaction to achieve specific production purposes, so as to reduce the use of chemical fertilizers and pesticides, is an important research approach in the development of green agriculture. To demonstrate the importance of rhizosphere microbial communities and guide practical production applications, this review summarizes the outstanding performance of rhizosphere microbial communities in promoting plant growth and stress tolerance. We also discuss the effect of host plants on their rhizosphere microbes, especially emphasizing the important role of host plant species and genes in the specific recruitment of beneficial microorganisms to improve the plants’ own traits. The aim of this review is to provide valuable insights into developing plant varieties that can consistently recruit specific beneficial microorganisms to improve crop adaptability and productivity, and thus can be applied to green and sustainable agriculture in the future.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100322"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of extracellular chitin deacetylase from Aneurinibacillus aneurinilyticus isolated from marine crustacean shell","authors":"Poonam Das ,&nbsp;Manisha Das ,&nbsp;Sheela Kumari Sahoo ,&nbsp;Jagneshwar Dandapat ,&nbsp;Jyotsnarani Pradhan","doi":"10.1016/j.crmicr.2024.100325","DOIUrl":"10.1016/j.crmicr.2024.100325","url":null,"abstract":"<div><div>Chitosan is a promising biopolymer with wide range of applications. It is the deacetylated product of chitin. Commercially, it is produced from chitin via a harsh thermochemical process that has several shortcomings and heterogenous deacetylation product. Chitin can be transformed into chitosan through enzymatic deacetylation using chitin deacetylase (CDA), enabling the production of chitosan with a specific degree of deacetylation. CDA is primarily extracted from fungi followed by bacteria and insects. The extraction of CDA from fungus is more complex, possess several health risks for human including skin lesions. Therefore, screening of potent bacterial CDA is the need of the hour. In this study, for the first time we have isolated a bacterial strain Aneurinibacillus aneurinilyticus from the rinsed water of marine crab shell, and it was found to be a potent CDA producer. The extracellular CDA from <em>A. aneurinilyticus</em> has been partially purified and the specific activity of the enzyme was found to be 569.73 U/ mg protein. SDS-PAGE profiling of the purified sample depicts two isomers of CDA with molecular weights of 27 kD and 45 kD. The pH and temperature optima of the purified CDA were found to be 7.4 and 37 °C, respectively. The partially purified enzyme has Km and Vmax values of 98.455 µM and 909.09 µmole/min, for non-chitinous substrate such as p-nitroacetanilide. For chitinous substrates like glycol chitin, N-acetyl glucosamine hexamer and colloidal chitin, the enzyme exhibited K_m of 96.96, 111.75 and 127.86 µM, respectively, V_max for these substrates were 23.31, 10.12 and 10.772 µmole/min, respectively. Metal ions like Mn and Mg considerably boost the production and activity of CDA, whereas Cd and Co strongly inhibit its activity. Insights from this study further substantiate that this enzyme follows Michaelis-Menten equation and has potential for industrial applications.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100325"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing cryptococcal treatment: The role of nanoparticles in mitigating antifungal resistance","authors":"Rahul Harikumar Lathakumari,&nbsp;Leela Kakithakara Vajravelu,&nbsp;Abhishek Satheesan,&nbsp;Jayaprakash Thulukanam","doi":"10.1016/j.crmicr.2024.100323","DOIUrl":"10.1016/j.crmicr.2024.100323","url":null,"abstract":"<div><div><em>Cryptococcus</em>, a ubiquitous and formidable fungal pathogen, contributes to a substantial global disease burden, with nearly 250,000 cases and 181,000 fatalities attributed to cryptococcal meningitis annually worldwide. The invasive nature of <em>Cryptococcus</em> presents significant challenges in treatment and management, as it mostly affects vulnerable populations, including HIV patients, organ transplant recipients, pregnant women, and elderly individuals. Moreover, these difficulties are exacerbated by the development of antifungal resistance, which emphasizes the need for efficient control measures. In this context, research efforts focusing on infection control and novel therapeutic strategies become paramount. Nanoparticle-based therapies emerge as a solution, offering advanced antifungal properties and improved efficacy. Developing effective treatment options requires understanding the complex landscape of cryptococcal infections and the innovative potential of nanoparticle-based therapies. This review highlights the urgent need for novel strategies to combat the growing threat posed by antifungal resistance while offering insights into the intricate realm of cryptococcal infections, particularly focusing on the promising role of nanoparticle-based therapies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100323"},"PeriodicalIF":4.8,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142719807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abscesses due to Melioidosis: A case-based review","authors":"Nitin Gupta ,&nbsp;Sundeep Malla ,&nbsp;Carl Boodman ,&nbsp;Tirlangi Praveen Kumar ,&nbsp;Muralidhar Varma ,&nbsp;Chiranjay Mukhopadhyay","doi":"10.1016/j.crmicr.2024.100321","DOIUrl":"10.1016/j.crmicr.2024.100321","url":null,"abstract":"<div><div>Melioidosis is caused by percutaneous inoculation or inhalation of <em>Burkholderia pseudomallei</em>, predominantly among individuals with risk factors (diabetes mellitus, immunosuppression, etc.) from endemic areas of South Asia, Southeast Asia and Northern Australia. While some patients present acutely with sepsis and multi-organ failure, others present with a subacute to chronic course characterised by abscess formation. We present nine representative cases, each with an abscess at a separate site (lung, skin, bone/ joint, prostate, parotid gland, liver, spleen, brain and orbits). Using these cases as examples, we reviewed the literature on abscesses in various organs.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100321"},"PeriodicalIF":4.8,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifaceted virus-like particles: Navigating towards broadly effective influenza A virus vaccines.","authors":"Jaffar Ali Muhamad Norizwan, Wen Siang Tan","doi":"10.1016/j.crmicr.2024.100317","DOIUrl":"10.1016/j.crmicr.2024.100317","url":null,"abstract":"<p><p>The threat of influenza A virus (IAV) remains an annual health concern, as almost 500,000 people die each year due to the seasonal flu. Current flu vaccines are highly dependent on embryonated chicken eggs for production, which is time consuming and costly. These vaccines only confer moderate protections in elderly people, and they lack cross-protectivity; thereby requiring annual reformulation to ensure effectiveness against contemporary circulating strains. To address current limitations, new strategies are being sought, with great emphasis given on exploiting IAV's conserved antigens for vaccine development, and by using different vaccine technologies to enhance immunogenicity and expedite vaccine production. Among these technologies, there are growing pre-clinical and clinical studies involving virus-like particles (VLPs), as they are capable to display multiple conserved IAV antigens and augment their immune responses. In this review, we outline recent findings involving broadly effective IAV antigens and strategies to display these antigens on VLPs. Current production systems for IAV VLP vaccines are comprehensively reviewed. Pain-free methods for administration of IAV VLP vaccines through intranasal and transdermal routes, as well as the mechanisms in stimulating immune responses are discussed in detail. The future perspectives of VLPs in IAV vaccine development are discussed, particularly concerning their potentials in overcoming current immunological limitations of IAV vaccines, and their inherent advantages in exploring intranasal vaccination studies. We also propose avenues to expedite VLP vaccine production, as we envision that there will be more clinical trials involving IAV VLP vaccines, leading to commercialization of these vaccines in the near future.</p>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"100317"},"PeriodicalIF":4.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of plant growth promoting rhizobacteria from cacti root under drought condition","authors":"Kumar Shreshtha ,&nbsp;Aman Prakash ,&nbsp;Prashant Kumar Pandey ,&nbsp;Arun Kumar Pal ,&nbsp;Jyotsna Singh ,&nbsp;Pooja Tripathi ,&nbsp;Debasis Mitra ,&nbsp;Durgesh Kumar Jaiswal ,&nbsp;Sergio de los Santos-Villalobos ,&nbsp;Vijay Tripathi","doi":"10.1016/j.crmicr.2024.100319","DOIUrl":"10.1016/j.crmicr.2024.100319","url":null,"abstract":"<div><div>Plant growth-promoting rhizobia (PGPR) helps plants grow and develop by protecting them from abiotic and biotic stresses, increasing the synthesis of chemicals that promote growth, and enabling the uptake of nutrients. Drought is one of the biggest problems throughout the world. The search for novel and efficient drought-resistant microorganisms that reduce the adverse effects executed by drought is a significant alternative. This study aimed to isolate and characterize PGPR strains from the <em>Opuntia Ficus-Indica</em> cactus plant's rhizosphere, cultivated in the semi-arid Shankargarh district of Uttar Pradesh, India. Tests for plant growth-promoting activity, such as the generation of indole acetic acid (IAA), phosphate solubilization, ammonia, carboxymethyl cellulase, and protease activity, were performed on all bacterial isolates. There were 246 bacterial strains isolated from the rhizospheric zone, and only 16.6 % showed drought resistance and various plant growth-promoting traits. The <em>Bacillus</em> sp. strain promoted the growth promotion of <em>Capsicum annum L.</em> under water stress (30 % field capacity). Additionally, <em>Bacillus sp</em>. isolates, with their potential for drought tolerance and plant growth promotion, could be applied in sustainable agriculture to enhance crop yield and resilience to water scarcity.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100319"},"PeriodicalIF":4.8,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142707373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}