Current Research in Microbial Sciences最新文献

{"title":"Reflections on microbial genetic resources in agricultural systems","authors":"Iván Pável Moreno-Espíndola,&nbsp;Alonso Gutiérrez-Navarro,&nbsp;Diana Carolina Franco-Vásquez,&nbsp;Daniel Vega-Martínez","doi":"10.1016/j.crmicr.2024.100337","DOIUrl":"10.1016/j.crmicr.2024.100337","url":null,"abstract":"<div><div>This paper reflects on the use of microbial genetic resources in the context of agricultural systems. The benefits of harnessing the diversity of these resources in any agricultural system are highlighted, as well as the importance of knowing and preserving native agrobiodiversity, which is deemed an essential resource for Latin America. In this region, harmful effects of irrational use of agrochemicals and monocultures on the environment, economy, and health have been brought to light. In view of the growing awareness and global interest in the development of bioinputs, rational biodiversity use, environmental crisis, cost of conventional synthetic inputs, and the right to safe and quality food, agriculture requires new tools and effective strategies in its practice. Microbial genetic resources are a potential source of relevant and suitable inputs for this purpose. Using the bibliometric technique of co-occurrence of terms with the VOSviewer tool, an analysis of 60 articles published between 2020 and 2024, collected from databases such as Scopus and Web of Science, was performed. An interest in microbial resources and their potential application in plant nutrition, production of growth regulators, defenses against pests and diseases, and tolerance to limiting environmental conditions can be appreciated. However, elucidating their complex ecological dynamics is necessary to understand them in real production contexts, thus allowing the allocation of relevant technological packages and the fair management of their use and potential benefits.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100337"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11751537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Process optimization and evaluation of quality properties of natto with co-culture of Bacillus subtilis natto and Limosilactobacillus fermentum","authors":"Yi-Ni Ma ,&nbsp;Xun Xu ,&nbsp;Lai-Feng Chen ,&nbsp;Jin-Ping Zhou ,&nbsp;Zhen-Hui Cao ,&nbsp;Qiu-Ye Lin","doi":"10.1016/j.crmicr.2025.100347","DOIUrl":"10.1016/j.crmicr.2025.100347","url":null,"abstract":"<div><div>The present study aimed to optimize the processing technology of natto fermented with <em>Bacillus subtilis</em> natto and <em>Limosilactobacillus fermentum</em> 2–2 and its effect on quality characteristics as well as volatile compounds. The results of single-factor tests and response surface methodology (RSM) showed that the optimal fermentation conditions of natto were L. <em>fermentum</em> inoculation amount of 0.3 %, fermentation temperature at 39 °C, fermentation time of 48 h, and after-ripening time of 24 h. Compared with natto control with <em>B. subtilis</em> natto only, natto co-cultured with <em>B. subtilis</em> natto and L. <em>fermentum</em> 2–2 showed higher scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and greater nattokinase activity. Volatomics analysis revealed that 71 differential volatile compounds were identified with 23 up-regulated and 48 down-regulated. Especially, co-fermentation of <em>B. subtilis</em> natto and L. <em>fermentum</em> 2–2 increased generation of alcohols, ketones and esters while reduced pyrazines formation. The above results indicate that co-culture of <em>B. subtilis</em> natto and L. <em>fermentum</em> can enhance the antioxidant and nattokinase activity and modify the volatile profile in natto and have the potential for application as the mixed starter in natto production.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100347"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First whole genome report of Mangrovibacter phragmitis PSU-3885–11 isolated from a patient in Thailand","authors":"Nattarika Chaichana ,&nbsp;Thunchanok Yaikhan ,&nbsp;Mingkwan Yingkajorn ,&nbsp;Nonthawat Thepsimanon ,&nbsp;Sirikan Suwannasin ,&nbsp;Kamonnut Singkhamanan ,&nbsp;Sarunyou Chusri ,&nbsp;Rattanaruji Pomwised ,&nbsp;Monwadee Wonglapsuwan ,&nbsp;Komwit Surachat","doi":"10.1016/j.crmicr.2025.100350","DOIUrl":"10.1016/j.crmicr.2025.100350","url":null,"abstract":"<div><div><em>Mangrovibacter phragmitis</em> is a Gram-negative bacterium typically found in plant roots that supports nitrogen fixation in nutrient-poor environments such as mangrove ecosystems. Although primarily found in environmental niches, an unusual case in Thailand of <em>M. phragmitis</em> strain PSU-3885–11 isolated from the sputum of a 29-year-old female patient with spinal tuberculosis. This isolate was initially misidentified as part of the <em>Enterobacter cloacae</em> complex (ECC) by MALDI-TOF. However, WGS subsequently confirmed its correct identity as <em>M. phragmitis</em>. The genome contains 4,651 coding sequences, along with 72 tRNA genes and 1 tmRNA. Moreover, comparative genomic analysis showed 99.32 % average nucleotide identity (ANI) similar to <em>M. phragmitis</em> MP23, and several antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs) were identified in the PSU-3885–11 genome which may contribute to its ability to survive in diverse environments, including human hosts. The PSU-3885–11 displayed resistance to beta-lactam antibiotics such as ampicillin and cefotaxime, while remaining sensitive to a wide range of other antibiotics. Key virulence genes including <em>ompA, hcp/tssD,</em> and <em>rpoS</em>, were identified which may play a role in its persistence in human hosts as an opportunistic pathogen. The presence of ribosomally synthesized and post-translationally modified peptides (RiPPs) and bacteriocins indicates the antimicrobial properties that may provide a competitive advantage in both environmental and clinical settings of this strain. Therefore, this study provides valuable insights into the genomic features, antibiotic resistance, and potential pathogenicity of <em>M. phragmitis</em> PSU-3885–11. The findings also emphasize the importance of continued surveillance and genomic analysis of environmental bacteria that may emerge as opportunistic pathogens in human infections.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100350"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multidrug resistant Acinetobacter baumannii: A study on its pathogenesis and therapeutics","authors":"Hridesh Mukhopadhyay ,&nbsp;Arnab Bairagi ,&nbsp;Anushka Mukherjee ,&nbsp;Aman Kumar Prasad ,&nbsp;Arjama Dhar Roy ,&nbsp;Aditi Nayak","doi":"10.1016/j.crmicr.2024.100331","DOIUrl":"10.1016/j.crmicr.2024.100331","url":null,"abstract":"<div><div>The overuse of antibiotics has led to the global dissemination of <em>Acinetobacter baumannii</em>, an increasingly challenging nosocomial pathogen. This review explores the medical significance along with the diverse resistance ability of <em>A. baumannii</em>. Intensive care units (ICUs) serve as a breeding ground for <em>A. baumannii</em>, as these settings harbour vulnerable patients and facilitate the spread of opportunistic microorganisms. <em>A. baumannii</em> belongs to the ESKAPE group of bacterial pathogens that are major contributors to antibiotic-resistant infections. The pathogenic nature of <em>A. baumannii</em> is particularly evident in seriously ill patients, causing pneumonia, wound infections, and other healthcare-associated infections. Historically considered benign, <em>A. baumannii</em> is a global threat due to its propensity for rapid acquisition of multidrug resistance phenotypes. The genus <em>Acinetobacter</em> was formally recognized in 1968 following a comprehensive survey by Baumann <em>et al</em>., highlighting the relationship between previously identified species and consolidating them under the name <em>Acinetobacter. A. baumannii</em> is characterized by its Gram-negative nature, dependence on oxygen, positive catalase activity, lack of oxidase activity, inability to ferment sugars, and non-motility. The DNA G+C content of <em>Acinetobacter</em> species falls within a specific range. For diagnostic purposes, <em>A. baumannii</em> can be cultured on specific agar media, producing distinct colonies. The genus <em>Acinetobacter</em> comprises numerous species those are associated with bloodstream infections with high mortality rates. Therefore, <em>A. baumannii</em> poses a significant challenge to global healthcare due to its multidrug resistance and ability to cause various infections. A comprehensive understanding of the mechanisms underlying its resistance acquisition and pathogenicity is essential for combating this healthcare-associated pathogen effectively.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100331"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11718326/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142972467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting","authors":"M Espejo Mambié ,&nbsp;D San Jose-Saras ,&nbsp;C Bischofberger Valdés ,&nbsp;C Díaz-Agero Pérez ,&nbsp;JC Galán Montemayor ,&nbsp;L Martínez-García ,&nbsp;M Abreu Di-Berardino ,&nbsp;P Moreno-Nunez ,&nbsp;J Vicente-Guijarro ,&nbsp;J.M Aranaz-Andrés","doi":"10.1016/j.crmicr.2025.100355","DOIUrl":"10.1016/j.crmicr.2025.100355","url":null,"abstract":"<div><h3>Background</h3><div>Demonstrating the capability to isolate biological material from the environment was fundamental to supporting any transmission route. Various and inconsistent methodologies have been used to address this issue; however, the debate in scientific societies about the possibility of airborne transmission as a source of SARS-CoV-2 spread remained open.</div></div><div><h3>Objective</h3><div>To analyze SARS-CoV-2 contamination in the air and on surfaces in a hospital setting during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div>This study involved air and surface sampling in the emergency, hospitalization, and intensive care unit areas of the Ramón y Cajal University Hospital. A consistent methodology was used for all samples, and clinical and environmental parameters and characterization of each location were recorded.</div></div><div><h3>Results</h3><div>A total of 234 samples were collected, comprising 160 surface samples and 74 air samples, of which 6.84 % tested positive (13/160 surface samples and 3/74 air samples). High-contact surfaces had the highest proportion of positive samples (12/13). All positive air samples were identified within 2 m of patients who had recently developed symptoms (&lt;5 days). High dependency and elevated temperatures seemed to indicate a higher risk of environmental biocontamination. Additionally, there was a higher risk of contamination in the intensive care units than in the hospitalization or emergency units.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100355"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics of Aspergillus nidulans and section Nidulantes","authors":"Sebastian Theobald ,&nbsp;Tammi Vesth ,&nbsp;Jane L. Nybo ,&nbsp;Jens C. Frisvad ,&nbsp;Inge Kjærbølling ,&nbsp;Stephen Mondo ,&nbsp;Kurt LaButti ,&nbsp;Sajeet Haridas ,&nbsp;Robert Riley ,&nbsp;Alan A. Kuo ,&nbsp;Asaf A. Salamov ,&nbsp;Jasmyn Pangilinan ,&nbsp;Anna Lipzen ,&nbsp;Maxim Koriabine ,&nbsp;Mi Yan ,&nbsp;Kerrie Barry ,&nbsp;Alicia Clum ,&nbsp;Ellen K. Lyhne ,&nbsp;Elodie Drula ,&nbsp;Ad Wiebenga ,&nbsp;Mikael R. Andersen","doi":"10.1016/j.crmicr.2025.100342","DOIUrl":"10.1016/j.crmicr.2025.100342","url":null,"abstract":"<div><div><em>Aspergillus nidulans</em> is an important model organism for eukaryotic biology and the reference for the section <em>Nidulantes</em> in comparative studies. In this study, we <em>de novo</em> sequenced the genomes of 25 species of this section. Whole-genome phylogeny of 34 <em>Aspergillus</em> species and <em>Penicillium chrysogenum</em> clarifies the position of clades inside section <em>Nidulantes</em>. Comparative genomics reveals a high genetic diversity between species with 684 up to 2433 unique protein families. Furthermore, we categorized 2118 secondary metabolite gene clusters (SMGC) into 603 families across Aspergilli, with at least 40 % of the families shared between <em>Nidulantes</em> species. Genetic dereplication of SMGC and subsequent synteny analysis provides evidence for horizontal gene transfer of a SMGC. Proteins that have been investigated in <em>A. nidulans</em> as well as its SMGC families are generally present in the section <em>Nidulantes</em>, supporting its role as model organism. The set of genes encoding plant biomass-related CAZymes is highly conserved in section <em>Nidulantes</em>, while there is remarkable diversity of organization of MAT-<em>loci</em> both within and between the different clades. This study provides a deeper understanding of the genomic conservation and diversity of this section and supports the position of <em>A. nidulans</em> as a reference species for cell biology.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100342"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea","authors":"Marianna Marangi ,&nbsp;Sonia Boughattas","doi":"10.1016/j.crmicr.2025.100349","DOIUrl":"10.1016/j.crmicr.2025.100349","url":null,"abstract":"<div><div><em>Blastocystis</em> sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on <em>Blastocystis</em> sp. distribution and its circulating subtypes (STs) from the terrestrial environment are available, while those from the marine environment remain still scare. A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to <em>Blastocystis</em> sp. using molecular tools. A predominance of zoonotic subtype ST3 among different species of marine mammals as well as the presence of ST1 allele 4 subtype and even untypable subtype within the fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes and Proteobacteria within the different <em>Blastocystis</em>-carrier marine mammal species as well the identification of Archaebacteria from Methanomethylophilaceae family within the fin whale isolate were detected by Illumina V3-V4 generated data. The present survey presents new insights regarding <em>Blastocystis</em> sp. prevalence and its circulating zoonotic ST1-ST3 subtypes from the marine environment, as well as its associated gut microbiome, providing hence baseline data for a better understanding of the associated risk and to prevent human and marine ecosystem exposure to these anthropogenic microorganisms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100349"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial potential and phytochemical analysis of two ethnomedicinally important plants","authors":"Narpat Singh,&nbsp;Neha Chhangani,&nbsp;Sharad Bissa","doi":"10.1016/j.crmicr.2024.100297","DOIUrl":"10.1016/j.crmicr.2024.100297","url":null,"abstract":"<div><div>Medicinal plants exhibited great role in drug industries. Herbal medicines and their derivative products are often prepared from crude plant extracts. <em>Echinops echinatus</em> and <em>Tridax procumbens</em> both are belonging to Asteraceae family and these plants are ethnomedicinally important due to their utilization as traditional medicine to cure various diseases. Aim of the current study is to evaluate the antimicrobial properties, preliminary phytochemical and GC-MS analysis of these ethnomedicinally important plants to identify the compounds which are responsible for antimicrobial properties. Their extracts exhibited antimicrobial activity against <em>Enterobacter aerogenes, Escherichia coli, Agrobacterium tumefaciens, Staphylococcus aureus, Bacillus subtilis, Pseudomonas syringae</em> and <em>Pseudomonas putida</em>. Both plants contain the active principles like flavonoids, alkaloids, glycosides, saponins, terpenoids and tannins. Result of GC-MS analysis showed the presence of many compounds such as n-Hexadecanoic acid, Hexadecanoic Acid, Methyl ester, Octadecanoic acid, Stigmasterol, Naphthalene, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-,5 Hydroxymethylfurfural, Lupeol, Dodecanoic acid, Vitamin E (α-Tocopherol), Neophytadiene, Phytol and many other compounds. These compounds are responsible for antimicrobial, anticancer and medicinal properties.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100297"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11681831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unearthing the burden of melioidosis in North India – an emerging threat in a non-endemic region","authors":"Shweta Raina ,&nbsp;Disha Gautam ,&nbsp;Rohit Kumar ,&nbsp;Kavita Sisodia ,&nbsp;Chiranjay Mukhopadhyay ,&nbsp;Harpreet Kaur ,&nbsp;Mohammed Ashiq ,&nbsp;Rushika Saksena","doi":"10.1016/j.crmicr.2025.100344","DOIUrl":"10.1016/j.crmicr.2025.100344","url":null,"abstract":"<div><div><em>Burkholderia pseudomallei (B. pseudomallei)</em> is the causative agent of the high-mortality disease called melioidosis. It is a severe infection that can be misdiagnosed due to variable presentation and low awareness among clinicians of the disease. It is endemic in India and well-described in southern and eastern coastal states. In the last decade, sporadic cases of melioidosis have been diagnosed in North Indian states, predominantly Rajasthan and Gujarat. The reported cases highlight the many risk factors for infection in this region that was not previously recognised as being endemic for melioidosis, including high prevalence of diabetes mellitus, alcoholism, and large rural population engaged in paddy cultivation. Climate change results in frequent flooding and waterlogging in urban areas, leading to exposure of soil harbouring <em>B. pseudomallei,</em> thus a contributing factor to the rise in cases in cities. As North India has not previously been considered an endemic region for melioidosis, wider awareness amongst clinicians and laboratorians is essential for early identification of symptoms, testing for <em>B. pseudomallei</em> in microbiology laboratories, and timely management of the disease to save lives lost to misdiagnosis. The present article describes various aspects of melioidosis in North India including diverse clinical manifestations, risk factors, and possible reasons for misdiagnosis and underreporting.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100344"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic wounds and adaptive Pseudomonas aeruginosa: A phenotypic and genotypic characterization","authors":"Kasandra Buchholtz ,&nbsp;Rie Jønsson ,&nbsp;Rasmus L. Marvig ,&nbsp;Biljana Mojsoska ,&nbsp;Karen Angeliki Krogfelt","doi":"10.1016/j.crmicr.2025.100348","DOIUrl":"10.1016/j.crmicr.2025.100348","url":null,"abstract":"<div><div>Phenotypic and genetic diversity is found in varying prevalence in clinical populations where beneficial adaptations enable the bacteria to avoid recognition and eradication by the host immune system. This study aimed to investigate the presence of <em>Pseudomonas aeruginosa</em> in chronic venous leg ulcers wounds over an 8-week time course. This was performed using genomic and phenotypic approaches to understand the survival and persistence of <em>Pseudomonas</em> strains. The findings of this study show that the two patients were colonized with a recurring <em>P. aeruginosa</em> genotype with only minor phenotypic differences and few SNP differences, suggesting that the <em>Pseudomonas</em> isolates present in the wound can survive and proliferate in the host's hostile environment. The results provided from this study will allow us to understand <em>P. aeruginosa</em> colonization during a 8 week time period.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100348"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}