Current Research in Microbial Sciences最新文献

{"title":"The composition and structure of bacterial and fungal communities in kiwifruit are influenced by photoselective nets","authors":"Paulo Fernandes ,&nbsp;Concha Cano-Díaz ,&nbsp;Rui Pinto ,&nbsp;Isabel Mourão ,&nbsp;Luís Miguel Brito ,&nbsp;Luísa Moura","doi":"10.1016/j.crmicr.2025.100424","DOIUrl":"10.1016/j.crmicr.2025.100424","url":null,"abstract":"<div><div>Photoselective nets in agriculture are typically designed to modify the light spectrum, intensity, and microclimate around crops, influencing plant growth, productivity, and quality. However, knowledge regarding their impact on the microbiota of plants and fruits remains limited. This study assessed the impact of pearl, grey, and yellow photoselective nets on the microbial communities present on kiwifruit surfaces using amplicon high-throughput sequencing of ITS and 16S metagenomic DNA. Kiwifruit pathogens associated with postharvest rot, such as <em>Alternaria, Didymella</em>, and <em>Cladosporium</em>, were significantly more prevalent on kiwis grown without nets. Additionally, different net types influenced microbial diversity, richness, and network structure. Pearl nets promoted bacterial richness and fungal diversity, while yellow nets enhanced overall diversity and resilience in both microbial communities. Grey nets resulted in evenness in fungal communities but led to less robust bacterial networks. Kiwifruit yield increased under photoselective nets compared to outside. At harvest, fruit dry matter, firmness, pH, total soluble solids, and titratable acidity were similar across treatments. However, fruits under yellow and grey nets showed significantly lower firmness compared to other treatments. Understanding these effects may contribute to optimizing fruit production and shelf-life management.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100424"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convalescent plasma therapy and long-term SARS-COV-2 antiviral immune response in a prospective cohort of patients with COVID-19","authors":"Aldo Barrera ,&nbsp;Constanza Martínez-Valdebenito ,&nbsp;Bruno Nervi ,&nbsp;Aracelly Gaete-Argel ,&nbsp;Nicolás M.S. Gálvez ,&nbsp;Catalina Osses ,&nbsp;Cecilia Vizcaya ,&nbsp;María E. Ceballos ,&nbsp;Jaime Pereira ,&nbsp;Mayling Chang ,&nbsp;Luis Rojas ,&nbsp;Sebastián Mondaca ,&nbsp;Carolina Henríquez ,&nbsp;Alexis M. Kalergis ,&nbsp;Alessandro Sette ,&nbsp;Alba Grifoni ,&nbsp;Ricardo Soto-Rifo ,&nbsp;Fernando Valiente-Echeverría ,&nbsp;Marcela Ferres ,&nbsp;María E. Balcells ,&nbsp;Nicole Le Corre","doi":"10.1016/j.crmicr.2025.100440","DOIUrl":"10.1016/j.crmicr.2025.100440","url":null,"abstract":"<div><div>During the SARS-CoV-2 pandemic, the use of convalescent plasma (CP) in high-risk patients was proposed and widely implemented in several countries as a potential COVID-19 therapy. Nonetheless, CP therapy’s impact on immune response is nowadays poorly understood, including the correlation between IgG levels, neutralization capacity, and cellular immune response against SARS-CoV-2. Here we evaluated, in a cohort of patients with COVID-19 requiring hospitalization and having received or not CP, as well as in CP donors (recovered from mild disease), the humoral and cellular immune response assessed by titers of SARS-CoV-2 IgG, neutralizing antibodies, and IFN-γ⁺/IL-2⁺ ELISpot during the first month (early) and up to nine months (long-term) after symptom onset. Results showed higher seropositivity and seroconversion rates between 7–12 days after plasma infusion in CP-recipients. However, similar IgG and neutralizing immune response kinetics between CP-recipients and non-recipients was observed during the first and until the ninth month of analysis. A positive correlation between IgG and neutralizing levels was detected. Compared to outpatient donors, hospitalized individuals showed a higher response at 3 and 6 months after symptoms onset. A sustained SARS-CoV-2-specific CD4⁺ and CD8⁺ T cell response was observed in outpatients and hospitalized patients, regardless of the CP treatment. We concluded that the CP infusion did not affect the long-term SARS-CoV-2 specific humoral and cellular immune responses. Nonetheless, CP may provide a therapeutic window by promoting a higher humoral response during the acute phase of COVID-19.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100440"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Global genomic epidemiology and plasmid-mediated dissemination of blaKPC and blaNDM in the Serratia marcescens complex” [Current Research in Microbial Sciences Volume 9 (2025) 100436]","authors":"Xiao Liu ,&nbsp;Zhen Liu ,&nbsp;Keyi Yu ,&nbsp;Zhenzhou Huang ,&nbsp;He Gao ,&nbsp;Xuemei Bai ,&nbsp;Zhiwen Sun ,&nbsp;Qiang Wei ,&nbsp;Duochun Wang","doi":"10.1016/j.crmicr.2025.100441","DOIUrl":"10.1016/j.crmicr.2025.100441","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100441"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial potential and phytochemical analysis of two ethnomedicinally important plants","authors":"Narpat Singh,&nbsp;Neha Chhangani,&nbsp;Sharad Bissa","doi":"10.1016/j.crmicr.2024.100297","DOIUrl":"10.1016/j.crmicr.2024.100297","url":null,"abstract":"<div><div>Medicinal plants exhibited great role in drug industries. Herbal medicines and their derivative products are often prepared from crude plant extracts. <em>Echinops echinatus</em> and <em>Tridax procumbens</em> both are belonging to Asteraceae family and these plants are ethnomedicinally important due to their utilization as traditional medicine to cure various diseases. Aim of the current study is to evaluate the antimicrobial properties, preliminary phytochemical and GC-MS analysis of these ethnomedicinally important plants to identify the compounds which are responsible for antimicrobial properties. Their extracts exhibited antimicrobial activity against <em>Enterobacter aerogenes, Escherichia coli, Agrobacterium tumefaciens, Staphylococcus aureus, Bacillus subtilis, Pseudomonas syringae</em> and <em>Pseudomonas putida</em>. Both plants contain the active principles like flavonoids, alkaloids, glycosides, saponins, terpenoids and tannins. Result of GC-MS analysis showed the presence of many compounds such as n-Hexadecanoic acid, Hexadecanoic Acid, Methyl ester, Octadecanoic acid, Stigmasterol, Naphthalene, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-,5 Hydroxymethylfurfural, Lupeol, Dodecanoic acid, Vitamin E (α-Tocopherol), Neophytadiene, Phytol and many other compounds. These compounds are responsible for antimicrobial, anticancer and medicinal properties.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100297"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11681831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics of Aspergillus nidulans and section Nidulantes","authors":"Sebastian Theobald ,&nbsp;Tammi Vesth ,&nbsp;Jane L. Nybo ,&nbsp;Jens C. Frisvad ,&nbsp;Inge Kjærbølling ,&nbsp;Stephen Mondo ,&nbsp;Kurt LaButti ,&nbsp;Sajeet Haridas ,&nbsp;Robert Riley ,&nbsp;Alan A. Kuo ,&nbsp;Asaf A. Salamov ,&nbsp;Jasmyn Pangilinan ,&nbsp;Anna Lipzen ,&nbsp;Maxim Koriabine ,&nbsp;Mi Yan ,&nbsp;Kerrie Barry ,&nbsp;Alicia Clum ,&nbsp;Ellen K. Lyhne ,&nbsp;Elodie Drula ,&nbsp;Ad Wiebenga ,&nbsp;Mikael R. Andersen","doi":"10.1016/j.crmicr.2025.100342","DOIUrl":"10.1016/j.crmicr.2025.100342","url":null,"abstract":"<div><div><em>Aspergillus nidulans</em> is an important model organism for eukaryotic biology and the reference for the section <em>Nidulantes</em> in comparative studies. In this study, we <em>de novo</em> sequenced the genomes of 25 species of this section. Whole-genome phylogeny of 34 <em>Aspergillus</em> species and <em>Penicillium chrysogenum</em> clarifies the position of clades inside section <em>Nidulantes</em>. Comparative genomics reveals a high genetic diversity between species with 684 up to 2433 unique protein families. Furthermore, we categorized 2118 secondary metabolite gene clusters (SMGC) into 603 families across Aspergilli, with at least 40 % of the families shared between <em>Nidulantes</em> species. Genetic dereplication of SMGC and subsequent synteny analysis provides evidence for horizontal gene transfer of a SMGC. Proteins that have been investigated in <em>A. nidulans</em> as well as its SMGC families are generally present in the section <em>Nidulantes</em>, supporting its role as model organism. The set of genes encoding plant biomass-related CAZymes is highly conserved in section <em>Nidulantes</em>, while there is remarkable diversity of organization of MAT-<em>loci</em> both within and between the different clades. This study provides a deeper understanding of the genomic conservation and diversity of this section and supports the position of <em>A. nidulans</em> as a reference species for cell biology.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100342"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea","authors":"Marianna Marangi ,&nbsp;Sonia Boughattas","doi":"10.1016/j.crmicr.2025.100349","DOIUrl":"10.1016/j.crmicr.2025.100349","url":null,"abstract":"<div><div><em>Blastocystis</em> sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on <em>Blastocystis</em> sp. distribution and its circulating subtypes (STs) from the terrestrial environment are available, while those from the marine environment remain still scare. A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to <em>Blastocystis</em> sp. using molecular tools. A predominance of zoonotic subtype ST3 among different species of marine mammals as well as the presence of ST1 allele 4 subtype and even untypable subtype within the fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes and Proteobacteria within the different <em>Blastocystis</em>-carrier marine mammal species as well the identification of Archaebacteria from Methanomethylophilaceae family within the fin whale isolate were detected by Illumina V3-V4 generated data. The present survey presents new insights regarding <em>Blastocystis</em> sp. prevalence and its circulating zoonotic ST1-ST3 subtypes from the marine environment, as well as its associated gut microbiome, providing hence baseline data for a better understanding of the associated risk and to prevent human and marine ecosystem exposure to these anthropogenic microorganisms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100349"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The endophytic fungus Cosmosporella sp. VM-42 from Vinca minor is a source of bioactive compounds with potent activity against drug-resistant bacteria","authors":"Ting He ,&nbsp;Xiao Li ,&nbsp;Rosario del Carmen Flores-Vallejo ,&nbsp;Ana-Maria Radu ,&nbsp;Jan Maarten van Dijl ,&nbsp;Kristina Haslinger","doi":"10.1016/j.crmicr.2025.100390","DOIUrl":"10.1016/j.crmicr.2025.100390","url":null,"abstract":"<div><div>Medicinal plants serve as valuable resources for the isolation of endophytic fungi. <em>Vinca minor</em> is a well-known producer of important vinca alkaloids and emerges as a promising source of endophytic fungi with antibacterial potential and biosynthetic capacity. In this study, we isolated an endophytic fungus from <em>V. minor</em> and identified it as <em>Cosmosporella</em> sp. VM-42<em>.</em> To date, relatively little is known about this fungal genus. The ethyl acetate extract of this isolate selectively inhibited Gram-positive bacteria, such as methicillin-sensitive and methicillin-resistant <em>Staphylococcus aureus</em> (MSSA and MRSA). Therefore, we isolated the most abundant compound from the crude extract and identified it as nectriapyrone with MIC and MBC values ranging from 125 to 62.5 µg/mL against MSSA and MRSA strains. We further sequenced and annotated the 39.07 Mb genome of the isolate, revealing that it encodes 9842 protein-coding genes, including 415 genes for carbohydrate-active enzymes and various biosynthetic gene clusters. Our untargeted metabolomic analysis shows that the fungus produces various secondary metabolites, including cyclodepsipeptides, dimeric naphtho-γ-pyrones, and macrolactones, which are known to have antifungal and antibacterial activities. In addition, we used small-molecule epigenetic modulators to activate the expression of silent biosynthetic gene clusters to broaden the chemical profile of <em>Cosmosporella</em> sp. VM-42<em>.</em> Taken together, we provide a first systematic analysis of <em>Cosmosporella</em> sp. VM-42, and our results show that it is a promising source of compounds with pharmacological potential against drug resistant bacteria.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100390"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of potentially microcystin-producing Microcystis in the Dominican Republic","authors":"Alfaniris Vargas Fernandez ,&nbsp;Patri Ramírez Ramirez ,&nbsp;Iris García Cruz ,&nbsp;Claudia Reyes Gutierrez ,&nbsp;Adrián Gutiérrez Cepeda ,&nbsp;Alexander Valdez Disla ,&nbsp;Roel Alejandro Chávez Luzania ,&nbsp;Sergio de los Santos Villalobos ,&nbsp;Pedro Leao ,&nbsp;Vitor Vasconcelos","doi":"10.1016/j.crmicr.2025.100389","DOIUrl":"10.1016/j.crmicr.2025.100389","url":null,"abstract":"<div><div>When the amount of nutrients in water bodies increases, cyanobacteria tend to proliferate rapidly in freshwater lakes and reservoirs, which can trigger cyanobacterial blooms. This increases the risk of cyanotoxin generation in water sources intended for human consumption, crop irrigation, and livestock. This study focused on identifying the presence of cyanobacteria and cyanotoxins in the Valdesia reservoir, which supplies drinking water to approximately 4 million people in Santo Domingo, Azua, San Cristóbal, San José de Ocoa, and Peravia in the Dominican Republic. Morphological observation suggested the presence of the genus <em>Microcystis</em>, which was confirmed by amplification and sequencing of two fragments of the 16S rRNA gene, as well as a fragment of the <em>mcyA</em> gene involved in encoding microcystins. This is the first report to highlight the urgent need to establish continuous monitoring of potentially microcystins-producing <em>Microcystis</em> sp. in this important reservoir, to implement appropriate water management measures to prevent their negative impact on public health and the environment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100389"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Reflexions on the role, diversity, conservation and management of the genetic microbial resources in Agriculture”","authors":"María del Pilar Rodríguez Guzmán ,&nbsp;Ismael Fernando Chávez Díaz ,&nbsp;Lily Xochilt Zelaya Molina","doi":"10.1016/j.crmicr.2025.100365","DOIUrl":"10.1016/j.crmicr.2025.100365","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100365"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting","authors":"M Espejo Mambié ,&nbsp;D San Jose-Saras ,&nbsp;C Bischofberger Valdés ,&nbsp;C Díaz-Agero Pérez ,&nbsp;JC Galán Montemayor ,&nbsp;L Martínez-García ,&nbsp;M Abreu Di-Berardino ,&nbsp;P Moreno-Nunez ,&nbsp;J Vicente-Guijarro ,&nbsp;J.M Aranaz-Andrés","doi":"10.1016/j.crmicr.2025.100355","DOIUrl":"10.1016/j.crmicr.2025.100355","url":null,"abstract":"<div><h3>Background</h3><div>Demonstrating the capability to isolate biological material from the environment was fundamental to supporting any transmission route. Various and inconsistent methodologies have been used to address this issue; however, the debate in scientific societies about the possibility of airborne transmission as a source of SARS-CoV-2 spread remained open.</div></div><div><h3>Objective</h3><div>To analyze SARS-CoV-2 contamination in the air and on surfaces in a hospital setting during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div>This study involved air and surface sampling in the emergency, hospitalization, and intensive care unit areas of the Ramón y Cajal University Hospital. A consistent methodology was used for all samples, and clinical and environmental parameters and characterization of each location were recorded.</div></div><div><h3>Results</h3><div>A total of 234 samples were collected, comprising 160 surface samples and 74 air samples, of which 6.84 % tested positive (13/160 surface samples and 3/74 air samples). High-contact surfaces had the highest proportion of positive samples (12/13). All positive air samples were identified within 2 m of patients who had recently developed symptoms (&lt;5 days). High dependency and elevated temperatures seemed to indicate a higher risk of environmental biocontamination. Additionally, there was a higher risk of contamination in the intensive care units than in the hospitalization or emergency units.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100355"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}