Current Research in Microbial Sciences最新文献_第2页

First report of potentially microcystin-producing Microcystis in the Dominican Republic 多米尼加共和国首次报告可能产生微囊藻毒素的微囊藻

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100389

Alfaniris Vargas Fernandez , Patri Ramírez Ramirez , Iris García Cruz , Claudia Reyes Gutierrez , Adrián Gutiérrez Cepeda , Alexander Valdez Disla , Roel Alejandro Chávez Luzania , Sergio de los Santos Villalobos , Pedro Leao , Vitor Vasconcelos

{"title":"First report of potentially microcystin-producing Microcystis in the Dominican Republic","authors":"Alfaniris Vargas Fernandez , Patri Ramírez Ramirez , Iris García Cruz , Claudia Reyes Gutierrez , Adrián Gutiérrez Cepeda , Alexander Valdez Disla , Roel Alejandro Chávez Luzania , Sergio de los Santos Villalobos , Pedro Leao , Vitor Vasconcelos","doi":"10.1016/j.crmicr.2025.100389","DOIUrl":"10.1016/j.crmicr.2025.100389","url":null,"abstract":"<div><div>When the amount of nutrients in water bodies increases, cyanobacteria tend to proliferate rapidly in freshwater lakes and reservoirs, which can trigger cyanobacterial blooms. This increases the risk of cyanotoxin generation in water sources intended for human consumption, crop irrigation, and livestock. This study focused on identifying the presence of cyanobacteria and cyanotoxins in the Valdesia reservoir, which supplies drinking water to approximately 4 million people in Santo Domingo, Azua, San Cristóbal, San José de Ocoa, and Peravia in the Dominican Republic. Morphological observation suggested the presence of the genus <em>Microcystis</em>, which was confirmed by amplification and sequencing of two fragments of the 16S rRNA gene, as well as a fragment of the <em>mcyA</em> gene involved in encoding microcystins. This is the first report to highlight the urgent need to establish continuous monitoring of potentially microcystins-producing <em>Microcystis</em> sp. in this important reservoir, to implement appropriate water management measures to prevent their negative impact on public health and the environment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100389"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting SARS-CoV-2病毒对医院环境的生物污染

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100355

M Espejo Mambié , D San Jose-Saras , C Bischofberger Valdés , C Díaz-Agero Pérez , JC Galán Montemayor , L Martínez-García , M Abreu Di-Berardino , P Moreno-Nunez , J Vicente-Guijarro , J.M Aranaz-Andrés

{"title":"Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting","authors":"M Espejo Mambié , D San Jose-Saras , C Bischofberger Valdés , C Díaz-Agero Pérez , JC Galán Montemayor , L Martínez-García , M Abreu Di-Berardino , P Moreno-Nunez , J Vicente-Guijarro , J.M Aranaz-Andrés","doi":"10.1016/j.crmicr.2025.100355","DOIUrl":"10.1016/j.crmicr.2025.100355","url":null,"abstract":"<div><h3>Background</h3><div>Demonstrating the capability to isolate biological material from the environment was fundamental to supporting any transmission route. Various and inconsistent methodologies have been used to address this issue; however, the debate in scientific societies about the possibility of airborne transmission as a source of SARS-CoV-2 spread remained open.</div></div><div><h3>Objective</h3><div>To analyze SARS-CoV-2 contamination in the air and on surfaces in a hospital setting during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div>This study involved air and surface sampling in the emergency, hospitalization, and intensive care unit areas of the Ramón y Cajal University Hospital. A consistent methodology was used for all samples, and clinical and environmental parameters and characterization of each location were recorded.</div></div><div><h3>Results</h3><div>A total of 234 samples were collected, comprising 160 surface samples and 74 air samples, of which 6.84 % tested positive (13/160 surface samples and 3/74 air samples). High-contact surfaces had the highest proportion of positive samples (12/13). All positive air samples were identified within 2 m of patients who had recently developed symptoms (<5 days). High dependency and elevated temperatures seemed to indicate a higher risk of environmental biocontamination. Additionally, there was a higher risk of contamination in the intensive care units than in the hospitalization or emergency units.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100355"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea 地中海西北部自由放养海洋哺乳动物单细胞微生物囊虫及其相关肠道微生物群的遗传多样性

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100349

Marianna Marangi , Sonia Boughattas

{"title":"Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea","authors":"Marianna Marangi , Sonia Boughattas","doi":"10.1016/j.crmicr.2025.100349","DOIUrl":"10.1016/j.crmicr.2025.100349","url":null,"abstract":"<div><div><em>Blastocystis</em> sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on <em>Blastocystis</em> sp. distribution and its circulating subtypes (STs) from the terrestrial environment are available, while those from the marine environment remain still scare. A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to <em>Blastocystis</em> sp. using molecular tools. A predominance of zoonotic subtype ST3 among different species of marine mammals as well as the presence of ST1 allele 4 subtype and even untypable subtype within the fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes and Proteobacteria within the different <em>Blastocystis</em>-carrier marine mammal species as well the identification of Archaebacteria from Methanomethylophilaceae family within the fin whale isolate were detected by Illumina V3-V4 generated data. The present survey presents new insights regarding <em>Blastocystis</em> sp. prevalence and its circulating zoonotic ST1-ST3 subtypes from the marine environment, as well as its associated gut microbiome, providing hence baseline data for a better understanding of the associated risk and to prevent human and marine ecosystem exposure to these anthropogenic microorganisms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100349"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative genomics of Aspergillus nidulans and section Nidulantes 空心曲霉和空心曲霉的比较基因组学研究。

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100342

Sebastian Theobald , Tammi Vesth , Jane L. Nybo , Jens C. Frisvad , Inge Kjærbølling , Stephen Mondo , Kurt LaButti , Sajeet Haridas , Robert Riley , Alan A. Kuo , Asaf A. Salamov , Jasmyn Pangilinan , Anna Lipzen , Maxim Koriabine , Mi Yan , Kerrie Barry , Alicia Clum , Ellen K. Lyhne , Elodie Drula , Ad Wiebenga , Mikael R. Andersen

{"title":"Comparative genomics of Aspergillus nidulans and section Nidulantes","authors":"Sebastian Theobald , Tammi Vesth , Jane L. Nybo , Jens C. Frisvad , Inge Kjærbølling , Stephen Mondo , Kurt LaButti , Sajeet Haridas , Robert Riley , Alan A. Kuo , Asaf A. Salamov , Jasmyn Pangilinan , Anna Lipzen , Maxim Koriabine , Mi Yan , Kerrie Barry , Alicia Clum , Ellen K. Lyhne , Elodie Drula , Ad Wiebenga , Mikael R. Andersen","doi":"10.1016/j.crmicr.2025.100342","DOIUrl":"10.1016/j.crmicr.2025.100342","url":null,"abstract":"<div><div><em>Aspergillus nidulans</em> is an important model organism for eukaryotic biology and the reference for the section <em>Nidulantes</em> in comparative studies. In this study, we <em>de novo</em> sequenced the genomes of 25 species of this section. Whole-genome phylogeny of 34 <em>Aspergillus</em> species and <em>Penicillium chrysogenum</em> clarifies the position of clades inside section <em>Nidulantes</em>. Comparative genomics reveals a high genetic diversity between species with 684 up to 2433 unique protein families. Furthermore, we categorized 2118 secondary metabolite gene clusters (SMGC) into 603 families across Aspergilli, with at least 40 % of the families shared between <em>Nidulantes</em> species. Genetic dereplication of SMGC and subsequent synteny analysis provides evidence for horizontal gene transfer of a SMGC. Proteins that have been investigated in <em>A. nidulans</em> as well as its SMGC families are generally present in the section <em>Nidulantes</em>, supporting its role as model organism. The set of genes encoding plant biomass-related CAZymes is highly conserved in section <em>Nidulantes</em>, while there is remarkable diversity of organization of MAT-<em>loci</em> both within and between the different clades. This study provides a deeper understanding of the genomic conservation and diversity of this section and supports the position of <em>A. nidulans</em> as a reference species for cell biology.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100342"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linalool exhibit antimicrobial ability against Elizabethkingia miricola by disrupting cellular and metabolic functions 芳樟醇通过破坏细胞和代谢功能，表现出对miricola elizabeth ethkingia的抗菌能力

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100380

Mingwang He , Weiming Zhong , Rongsi Dai , Su Long , Ying Zhou , Tongping Zhang , Boyang Zhou , Tao Tang , Linlin Yang , Sifan Jiang , Wenbin Xiao , YanJiao Fu , Jiajing Guo , Zhipeng Gao

{"title":"Linalool exhibit antimicrobial ability against Elizabethkingia miricola by disrupting cellular and metabolic functions","authors":"Mingwang He , Weiming Zhong , Rongsi Dai , Su Long , Ying Zhou , Tongping Zhang , Boyang Zhou , Tao Tang , Linlin Yang , Sifan Jiang , Wenbin Xiao , YanJiao Fu , Jiajing Guo , Zhipeng Gao","doi":"10.1016/j.crmicr.2025.100380","DOIUrl":"10.1016/j.crmicr.2025.100380","url":null,"abstract":"<div><div><em>Elizabethkingia miricola</em> is a gram-negative bacillus, a life-threatening pathogen in humans and animals. Linalool, a naturally occurring monoterpene alcohol found in plant volatile oils, exhibits highly effective antibacterial properties. This study investigated the antibacterial activity and mechanism of linalool against <em>E. miricola</em>. Initially, linalool showed potent antibacterial activity against <em>E. miricola</em>, with inhibition zone (ZOI), MIC, and MBC values of 36.41 ± 1.23 mm, 0.125 % (v/v, 1.0775 mg/mL), and 0.125 % (v/v, 1.0775 mg/mL), respectively. Secondly, it was observed by electron microscopy that linalool caused crumpling, depression, and size reduction of the cells. Linalool affected cell membrane integrity, causing membrane damage and rupture. Thirdly, transcriptome analysis suggested that linalool affected C5-branched-chain dicarboxylic acid metabolism and the biosynthesis of valine, leucine, and isoleucine, result in increased energy production to linalool stress. Linalool disrupted cell division and RNA function in <em>E. miricola</em>, and the cells responded to linalool-induced oxidative damage by up-regulating the expression of <em>msrB</em> and <em>katG</em> genes. Fourthly, metabolome analysis revealed an increase in metabolites related to the glycerophospholipid metabolic pathway and NADP content in <em>E. miricola</em>, which may be a metabolic response to linalool stress. Taken together, these findings provide a theoretical basis for the antibacterial mechanism of linalool and suggest potential applications for preventing <em>E. miricola</em> infections.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100380"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of production of pathogen-suppressing volatiles using amino acids 利用氨基酸增强病原体抑制挥发物的产生

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100385

Christina Papazlatani , Annabell Wagner , Zhijun Chen , Hans Zweers , Wietse de Boer , Paolina Garbeva

{"title":"Enhancement of production of pathogen-suppressing volatiles using amino acids","authors":"Christina Papazlatani , Annabell Wagner , Zhijun Chen , Hans Zweers , Wietse de Boer , Paolina Garbeva","doi":"10.1016/j.crmicr.2025.100385","DOIUrl":"10.1016/j.crmicr.2025.100385","url":null,"abstract":"<div><div>Bacterial volatile organic compounds can play a significant role in antagonistic interactions. Enhancing the production of bacterial volatiles that suppress the growth of soil-borne phytopathogenic fungi, has perspective as a sustainable disease control strategy. In the present study, we explored the potential of stimulating <em>Burkholderia</em> AD24 and <em>Paenibacillus</em> AD87 to produce volatiles that suppress the growth of the plant pathogenic fungi <em>Fusarium culmorum</em> PV and <em>Rhizoctonia solani</em> AG2.2IIIb. We provided the bacterial strains with a mixture of amino acids that can serve as precursor molecules in metabolic routes leading to emission of suppressive bacterial volatiles. Only <em>Burkholderia</em> AD24 was stimulated to produce a volatile blend that led to higher suppression of both pathogens. Subsequent analysis of the volatile composition emitted by <em>Burkholderia</em> AD24 in the presence of amino acids, showed higher abundance of antifungal compounds, including sulfur compounds (DMDS), pyrazines (2,5-dimethyl pyrazine) and carbohydrates (3-methyl-1-butanol). Follow-up trials with single amino acids revealed a pathogen specific response effect. When <em>Burkholderia</em> AD24 was cultivated in the presence of glutamine and asparagine, the emitted volatile blend suppressed the growth of <em>F. culmorum</em>, whereas when cultivated in the presence of glycine, glutamine, arginine and lysine the volatile blend suppressed the growth of <em>R. solani</em>. Analysis of the volatile blend composition showed differences between the amino acid treatments. Our findings show that amino acid precursor molecules can stimulate the production of fungistatic volatiles but the sensitivity of the fungal pathogens to these bacterial volatiles varies. This should be considered in future application strategies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100385"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Irrigation water and soil chemistry shape fungal guilds in date palm soils, enhancing pathotroph abundance under saline groundwater irrigation 灌溉水和土壤化学形成了枣椰树土壤中的真菌行会，提高了盐水灌溉条件下的病原菌丰度

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100370

Subha Chandran , Dinesh Sanka Loganathachetti , Balamurugan Sadaiappan , Sanjay Swarup , Sunil Mundra

{"title":"Irrigation water and soil chemistry shape fungal guilds in date palm soils, enhancing pathotroph abundance under saline groundwater irrigation","authors":"Subha Chandran , Dinesh Sanka Loganathachetti , Balamurugan Sadaiappan , Sanjay Swarup , Sunil Mundra","doi":"10.1016/j.crmicr.2025.100370","DOIUrl":"10.1016/j.crmicr.2025.100370","url":null,"abstract":"<div><div>Saline groundwater irrigation is a predominant practice, especially in date palm (<em>Phoenix dactylifera L</em>.) farms in arid agroecosystems with scarce freshwater resources. Despite its economic importance, the influence of saline groundwater irrigation on bulk soil fungi remains overlooked. This study examined how saline groundwater irrigation affects fungal diversity, community structure, and assembly processes. Bulk soils from date palm farms with distinct irrigation sources (freshwater and saline groundwater), were analysed, and fungal community analyses were done using the internal transcribed spacer 2 (ITS2) sequencing. Soils irrigated with saline groundwater had a lower percentage (27%) of unique operational taxonomic units (OTUs) compared to freshwater (33.3%). Fungal richness negatively correlated with soil pH. Differences observed in overall and guild-specific fungal communities, with irrigation water electrical conductivity (EC) emerging as a pivotal factor distinguishing between the two irrigation sources. Notably, pathotrophs abundance was significant in soils irrigated with saline groundwater. Furthermore, the dominant pathotroph <em>Fusarium</em>, exhibited drift-based assembly process and was observed to be high under saline groundwater irrigation. Our study reveals that groundwater salinity reduces the number of unique OTUs and alters fungal communities at the overall and guild levels. This insight aids agricultural improvement in regions where saline groundwater is a predominant water source.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100370"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Secondary Metabolites from Bacillus spp. probiotics as potential treatments for multidrug-resistant pathogens: A comprehensive review 芽孢杆菌属益生菌的次生代谢物作为多药耐药病原体的潜在治疗方法：综述

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100392

George Michael Nicolas

引用次数: 0

Canine-derived Weissella confusa ZJUIDS-D034 and Enterococcus faecalis ZJUIDS-D016 combat aging by regulating gut microbiota 犬源卫塞尔菌ZJUIDS-D034和粪肠球菌ZJUIDS-D016通过调节肠道微生物群来对抗衰老

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100381

Nan Chen , Zexu Yu , Xuan Ji , Siyi Zhang , Chongwei Yu , Teresa G. Valencak , Fushan Shi , Daxi Ren

{"title":"Canine-derived Weissella confusa ZJUIDS-D034 and Enterococcus faecalis ZJUIDS-D016 combat aging by regulating gut microbiota","authors":"Nan Chen , Zexu Yu , Xuan Ji , Siyi Zhang , Chongwei Yu , Teresa G. Valencak , Fushan Shi , Daxi Ren","doi":"10.1016/j.crmicr.2025.100381","DOIUrl":"10.1016/j.crmicr.2025.100381","url":null,"abstract":"<div><div>Old age raises the susceptibility of age-related disease in domestic dogs. Discovering effective anti-aging interventions is key for mitigating age-related disease and conserving “healthspan” in pet dogs. In this study, 2 bacterial strains were isolated from canine feces. After screening and identifying the strains, <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 were chosen to intervene during <em><span>d</span>-</em>galactose-induced senescence in mice. We found that administering <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 improved the aging phenotype of mice, including an increase in antioxidant activity, a decrease in pro-inflammatory cytokines, and the restoration of intestinal and liver tissue damage. In addition, <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 lead to changes in the structure of intestinal microbiota in aging mice. Specifically, there was a decrease in the abundance of the <em>Cyanobacteria</em> and an increase in the abundance of <em>Akkermansia</em> and <em>Lactobacillus</em>. More importantly, there was a significant increase in acetic acid, a short-chain fatty acid, due to intervention with the 2 strains. This increase might be attributed to higher <em>Akkermansia</em>. We show that the modulation of gut microbiota and metabolism in aging mice may be a promising strategy through which <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 might exert their anti-aging effects.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100381"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Large scale identification of pellicle and cell-free liquid phase associated proteins in Bacillus amyloliquefaciens L-17 解淀粉芽孢杆菌L-17胞膜及无细胞液相相关蛋白的大规模鉴定

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100387

Tassadit Ouidir , Julie Hardouin , Claire-Emmanuelle Marcato-Romain , Elisabeth Girbal-Neuhauser , Yassine Nait Chabane

{"title":"Large scale identification of pellicle and cell-free liquid phase associated proteins in Bacillus amyloliquefaciens L-17","authors":"Tassadit Ouidir , Julie Hardouin , Claire-Emmanuelle Marcato-Romain , Elisabeth Girbal-Neuhauser , Yassine Nait Chabane","doi":"10.1016/j.crmicr.2025.100387","DOIUrl":"10.1016/j.crmicr.2025.100387","url":null,"abstract":"<div><div><em>Bacillus amyloliquefaciens</em> is a soil-associated and plant growth-promoting bacterium. It is the focus of numerous studies due to its ability to sporulate, form biofilms, produce antimicrobial peptides and commercial enzymes. The ability of <em>B. amyloliquefaciens</em> <span>l</span>-17 to form floating biofilm at the air-liquid interface “pellicle” was previously demonstrated. This pellicle exhibits a highly structured architecture which is provided by loosely and tightly matrix bound polysaccharides and proteins. In this study, a first large scale proteomic investigation of both the pellicle and the cell-free liquid phase of <span>l</span>-17 strain was performed. An approach based on physical and chemical extraction of the pellicular matrix combined with protein analysis by mass spectrometry identified 87 weakly matrix-bound proteins and 62 tightly bound proteins. A total of 131 pellicle-associated proteins were identified, including (i) the conserved proteins TasA and TapA, involved in biofilm formation and cohesion (ii) BslA, important for biofilm hydrophobicity (iii) several enzymes that make nutrients available and protect the biofilm from competitors (iv) flagellin and (v) proteins involved in the sporulation process. Proteomic characterization of the cell-free liquid phase underlying the analyzed pellicle allowed the identification of 423 proteins including 118 proteins yet identified in the matrix of the pellicle. The proteins identified specifically in the liquid phase include enzymes involved in the biosynthesis process of non-ribosomal peptides and a variety of commercial enzymes such as proteases, lipases, aminotransferases, peroxidases and phytases. This provides valuable clues to promote the industrial and agricultural application of the cell-free liquid phase of <em>B. amyloliquefaciens</em> <span>l</span>-17.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100387"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0