Current Research in Microbial Sciences最新文献

{"title":"Corrigendum to “Global genomic epidemiology and plasmid-mediated dissemination of blaKPC and blaNDM in the Serratia marcescens complex” [Current Research in Microbial Sciences Volume 9 (2025) 100436]","authors":"Xiao Liu , Zhen Liu , Keyi Yu , Zhenzhou Huang , He Gao , Xuemei Bai , Zhiwen Sun , Qiang Wei , Duochun Wang","doi":"10.1016/j.crmicr.2025.100441","DOIUrl":"10.1016/j.crmicr.2025.100441","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100441"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144663687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering reveals the relative importance of different sugar catabolic pathways during consumption of plant biomass by Aspergillus niger","authors":"Tania Chroumpi ,&nbsp;Astrid Müller ,&nbsp;Mao Peng ,&nbsp;Mar Cubertorer Navarro ,&nbsp;Robin Kuijpers ,&nbsp;Agata Terebieniec ,&nbsp;Jiajia Li ,&nbsp;Lye Meng Markillie ,&nbsp;Hugh D. Mitchell ,&nbsp;Carrie D. Nicora ,&nbsp;Chelsea M. Hutchinson ,&nbsp;Vanessa Paurus ,&nbsp;Samuel O. Purvine ,&nbsp;Chaevien S. Clendinen ,&nbsp;Galya Orr ,&nbsp;Scott E. Baker ,&nbsp;Miia R. Mäkelä ,&nbsp;Ronald P. de Vries","doi":"10.1016/j.crmicr.2025.100454","DOIUrl":"10.1016/j.crmicr.2025.100454","url":null,"abstract":"<div><div>To evaluate the impact of individual sugar catabolic pathways on the physiology of <em>A. niger</em> when using plant biomass as a carbon source, key pathways converting plant biomass derived monomers were blocked. The resulting deletion mutants were analyzed using wheat bran and sugar beet pulp as substrates. On both substrates, the strongest affected single-pathway mutants were the pentose catabolic pathway (PCP) (Δ<em>xkiA</em>) and glycolysis (Δ<em>hxkA</em>Δ<em>glkA</em>) deficient mutants. On wheat bran, which is rich in pentose sugars, blocking the PCP by deletion of <em>xkiA</em> strongly impacted both growth and gene expression. However, the effect was even stronger in Δ<em>hxkA</em>Δ<em>glkA</em> and similar to a strain in which all pathways were blocked, highlighting the crucial role of glycolysis and/or carbon catabolite repression in <em>A. niger</em> physiology. These results demonstrate the complexity of <em>A. niger</em> metabolism during growth on plant biomass and provide insights into aspects to consider during metabolic engineering to obtain fungal cell factories.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100454"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144780312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold stress enhances cryotolerance in Lacticaseibacillus rhamnosus B6 via membrane lipid remodeling and differential protein expression","authors":"Ting Yang ,&nbsp;Jia Zhang ,&nbsp;Ranjin Zhang ,&nbsp;Juan Zhang ,&nbsp;Zhenmin Liu ,&nbsp;Zhengjun Wu","doi":"10.1016/j.crmicr.2025.100453","DOIUrl":"10.1016/j.crmicr.2025.100453","url":null,"abstract":"<div><div>Understanding the molecular mechanisms underlying the cryotolerance of lactic acid bacteria is critical for preserving their viability in food processing. In this study, <em>Lacticaseibacillus rhamnosus</em> B6 and KF7 with different phenotypic properties were pretreated at 4 °C for 2 h before liquid nitrogen freezing. Cold-stressed B6 exhibited significantly higher survival (53 %) than KF7 (30 %) and untreated controls (44 % vs. 10 %, <em>p</em> &lt; 0.05), with few disrupted cells observed under SEM. Cold stress altered the membrane fluidity in B6 by increasing unsaturated fatty acids (UFA) and the ratio of UFA to saturated fatty acids (UFA/SFA) from 1.36 to 1.62 (<em>p</em> &lt; 0.05). 219 proteins, primarily those involved in fatty acid biosynthesis, translation and transport processes were up regulated in cold-stressed B6, compared to untreated counterparts. Noteworthy, capsules of B6 likely contributed to its higher cryotolerance. Our findings revealed a dual cryotolerance mechanism in L. <em>rhamnosus</em> B6, i.e., dynamic lipidome remodeling and coordinated protein expression regulation. This study highlighted the importance of the cell surface features of individual strain of L. <em>rhamnosus</em> and cold stress treatment in preparing probiotics as well as direct to vat starter cultures with high cell vitality employing deep freezing process.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100453"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144831354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing prostate cancer treatment: the role of fecal microbiota transplantation as an adjuvant therapy","authors":"Rasoul Ebrahimi ,&nbsp;Shahrzad Shahrokhi Nejad ,&nbsp;Mehra Fekri ,&nbsp;Seyed Aria Nejadghaderi","doi":"10.1016/j.crmicr.2025.100420","DOIUrl":"10.1016/j.crmicr.2025.100420","url":null,"abstract":"<div><div>Prostate cancer (PCa) is a major cause of cancer-related deaths worldwide. While current treatments such as surveillance, surgery, and radiation are effective, they have their limitations. These can include patient incompliance due to side effects or resistance to hormonal changes, highlighting the need for alternative approaches. Human microbiota, a complex and dynamic host, plays a significant role in the homeostasis and is associated with several diseases or cancers in cases of dysregulation and dysbiosis. Research on fecal microbiota profiling and its association with certain cancers has opened new possibilities for preventing and managing tumor progression. One such possibility is fecal microbial transplantation (FMT). Studies show that different composition of urinary microbiota is found in various urinary tract diseases. Gut microbiota can regulate immune response against tumors; therefore, FMT may help modulate gut microbiota in a way that potentially enhances responses to immune checkpoint inhibitors, as suggested by emerging evidence in other cancers, though this needs further validation in PCa. Nevertheless, long-term complications and the safety of FMT are still questioned. We reviewed the roles of gut microbiota in PCa and suggested FMT as a potential tool in the treatment of PCa, which needs further investigations.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100420"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melioidosis cases detected in Dhaka, Bangladesh: a positive impact of 3rd South Asian melioidosis congress-2023","authors":"Farhan Muhib ,&nbsp;Maisha Islam ,&nbsp;Salman Ferdous Bhuiyan ,&nbsp;Mir Sajedul Karim ,&nbsp;Aunta Melan ,&nbsp;Kakali Halder ,&nbsp;Sarmin Satter ,&nbsp;Parama Aminul ,&nbsp;Fathma Farhana ,&nbsp;Fahmida Rahman ,&nbsp;Sazzad Bin Shahid ,&nbsp;Mohsina Khatoon ,&nbsp;Zahidul Hasan ,&nbsp;Fazle Rabbi Chowdhury ,&nbsp;Shariful Alam Jilani ,&nbsp;Saika Farook","doi":"10.1016/j.crmicr.2025.100464","DOIUrl":"10.1016/j.crmicr.2025.100464","url":null,"abstract":"<div><div>Melioidosis is a fatal infectious disease caused by the gram-negative soil dwelling bacterium <em>Burkholderia pseudomallei</em>. Bangladesh is considered a definite country for melioidosis owing to the isolation of the organism both from clinical samples as well as soil. Only around 100 cases of melioidosis have been diagnosed sporadically in the country for the past 60 years till October 2023 and about 90 % of these cases were detected in BIRDEM General Hospital. A dilemma existed for researchers worldwide whether the disease is actually rare in this part of the continent or whether it is the limitation of our clinicians and microbiologists keeping the disease neglected due to a lack of clinical and laboratory suspicion, knowledge gap and occasionally lack of adequate laboratory facilities. Consequently, to overcome these obstacles, a group of dedicated researchers began conducting workshops, seminars and finally arranged the 3rd South Asian Melioidosis Congress-2023 to disseminate knowledge on the fatality of the disease and ways to detect and manage the cases among the physicians all over the country. The outstanding outcome of these melioidosis centered educational sessions was the isolation and identification of nine melioidosis cases from different clinical settings of the country, other than BIRDEM. We present the nine melioidosis cases with different clinical manifestations. Interestingly, 33.3 % patients were non-diabetic, although two of the nine patients had other chronic illness, while a young patient of 28 years had no known co-morbidities at all. Eight of the nine patients recovered after six months treatment, while one patient unfortunately expired. The case series is expected to raise vigilance among the concerned physicians and microbiologists of the country to keep the suspicion of melioidosis at the back of their mind in high risk patients, thus improving the chance of early diagnoses and management.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100464"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the type IX secretion system: an exploration focusing on Riemerella anatipestifer","authors":"Pengfei Niu ,&nbsp;Rong Guo ,&nbsp;Chan Ding ,&nbsp;Shengqing Yu","doi":"10.1016/j.crmicr.2025.100404","DOIUrl":"10.1016/j.crmicr.2025.100404","url":null,"abstract":"<div><div>The type IX secretion system (T9SS) is specific to the Fibrobacteres-Chlorobi-Bacteroidetes superphylum, the members of which have many specialized features, including gliding motility and the transport of cargo proteins across the outer membrane. At least 20 proteins have been identified as the components of T9SS. Proteins secreted by T9SS use the Sec system to cross the inner membrane, and the conserved C-terminal domain targets them to the T9SS for delivery to the extracellular environment or attachment to the outer membrane. <em>Riemerella anatipestifer</em> is an important pathogen of waterfowl, causing septicemic and exudative diseases. A genetic analysis recently detected T9SS in <em>R. anatipestifer</em>, in which it transports proteins and affects virulence. Moreover, at least 19 T9SS-transported proteins in <em>R. anatipestifer</em> strain Yb2 were identified, with multiple functions and affecting <em>R. anatipestifer</em> in different ways. In this review, we focus on recent advances in our understanding of the structure, function, and organization of the T9SS machinery and new findings about this highly novel secretion system in <em>R. anatipestifer</em>.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100404"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144893496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The composition and structure of bacterial and fungal communities in kiwifruit are influenced by photoselective nets","authors":"Paulo Fernandes ,&nbsp;Concha Cano-Díaz ,&nbsp;Rui Pinto ,&nbsp;Isabel Mourão ,&nbsp;Luís Miguel Brito ,&nbsp;Luísa Moura","doi":"10.1016/j.crmicr.2025.100424","DOIUrl":"10.1016/j.crmicr.2025.100424","url":null,"abstract":"<div><div>Photoselective nets in agriculture are typically designed to modify the light spectrum, intensity, and microclimate around crops, influencing plant growth, productivity, and quality. However, knowledge regarding their impact on the microbiota of plants and fruits remains limited. This study assessed the impact of pearl, grey, and yellow photoselective nets on the microbial communities present on kiwifruit surfaces using amplicon high-throughput sequencing of ITS and 16S metagenomic DNA. Kiwifruit pathogens associated with postharvest rot, such as <em>Alternaria, Didymella</em>, and <em>Cladosporium</em>, were significantly more prevalent on kiwis grown without nets. Additionally, different net types influenced microbial diversity, richness, and network structure. Pearl nets promoted bacterial richness and fungal diversity, while yellow nets enhanced overall diversity and resilience in both microbial communities. Grey nets resulted in evenness in fungal communities but led to less robust bacterial networks. Kiwifruit yield increased under photoselective nets compared to outside. At harvest, fruit dry matter, firmness, pH, total soluble solids, and titratable acidity were similar across treatments. However, fruits under yellow and grey nets showed significantly lower firmness compared to other treatments. Understanding these effects may contribute to optimizing fruit production and shelf-life management.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100424"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144480469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Convalescent plasma therapy and long-term SARS-COV-2 antiviral immune response in a prospective cohort of patients with COVID-19","authors":"Aldo Barrera ,&nbsp;Constanza Martínez-Valdebenito ,&nbsp;Bruno Nervi ,&nbsp;Aracelly Gaete-Argel ,&nbsp;Nicolás M.S. Gálvez ,&nbsp;Catalina Osses ,&nbsp;Cecilia Vizcaya ,&nbsp;María E. Ceballos ,&nbsp;Jaime Pereira ,&nbsp;Mayling Chang ,&nbsp;Luis Rojas ,&nbsp;Sebastián Mondaca ,&nbsp;Carolina Henríquez ,&nbsp;Alexis M. Kalergis ,&nbsp;Alessandro Sette ,&nbsp;Alba Grifoni ,&nbsp;Ricardo Soto-Rifo ,&nbsp;Fernando Valiente-Echeverría ,&nbsp;Marcela Ferres ,&nbsp;María E. Balcells ,&nbsp;Nicole Le Corre","doi":"10.1016/j.crmicr.2025.100440","DOIUrl":"10.1016/j.crmicr.2025.100440","url":null,"abstract":"<div><div>During the SARS-CoV-2 pandemic, the use of convalescent plasma (CP) in high-risk patients was proposed and widely implemented in several countries as a potential COVID-19 therapy. Nonetheless, CP therapy’s impact on immune response is nowadays poorly understood, including the correlation between IgG levels, neutralization capacity, and cellular immune response against SARS-CoV-2. Here we evaluated, in a cohort of patients with COVID-19 requiring hospitalization and having received or not CP, as well as in CP donors (recovered from mild disease), the humoral and cellular immune response assessed by titers of SARS-CoV-2 IgG, neutralizing antibodies, and IFN-γ⁺/IL-2⁺ ELISpot during the first month (early) and up to nine months (long-term) after symptom onset. Results showed higher seropositivity and seroconversion rates between 7–12 days after plasma infusion in CP-recipients. However, similar IgG and neutralizing immune response kinetics between CP-recipients and non-recipients was observed during the first and until the ninth month of analysis. A positive correlation between IgG and neutralizing levels was detected. Compared to outpatient donors, hospitalized individuals showed a higher response at 3 and 6 months after symptoms onset. A sustained SARS-CoV-2-specific CD4⁺ and CD8⁺ T cell response was observed in outpatients and hospitalized patients, regardless of the CP treatment. We concluded that the CP infusion did not affect the long-term SARS-CoV-2 specific humoral and cellular immune responses. Nonetheless, CP may provide a therapeutic window by promoting a higher humoral response during the acute phase of COVID-19.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100440"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance and novel Sequence Types of Klebsiella spp. in human, animal, and food sources: a One Health perspective from Northern Nigeria","authors":"Ayodele Timilehin Adesoji ,&nbsp;Vittoria Mattioni Marchetti ,&nbsp;Claudia Cortimiglia ,&nbsp;Francesca Piscopiello ,&nbsp;Ilaria Petrizzi ,&nbsp;Aurora Piazza ,&nbsp;Emmanuel Dayo Alabi ,&nbsp;Pier Sandro Cocconcelli ,&nbsp;Roberta Migliavacca","doi":"10.1016/j.crmicr.2025.100458","DOIUrl":"10.1016/j.crmicr.2025.100458","url":null,"abstract":"&lt;div&gt;&lt;div&gt;This study aimed to evaluate the genomic characteristics of &lt;em&gt;Klebsiella&lt;/em&gt; spp. from human, animal, and food sources, and to establish their clonal relationships with globally circulating sequence types (STs). Using both phenotypic and next-generation sequencing approaches, a total of 300 samples comprising food samples (&lt;em&gt;n&lt;/em&gt; = 180) and human clinical samples (&lt;em&gt;n&lt;/em&gt; = 120) were collected and analyzed. Of these, 45 &lt;em&gt;Klebsiella&lt;/em&gt; spp. were isolated from human (&lt;em&gt;n&lt;/em&gt; = 16), animal (&lt;em&gt;n&lt;/em&gt; = 15), and food (&lt;em&gt;n&lt;/em&gt; = 14) sources. &lt;em&gt;K. pneumoniae&lt;/em&gt; was the predominant species (34/45; 75.5%), followed by &lt;em&gt;K. variicola&lt;/em&gt; subsp&lt;em&gt;. variicola&lt;/em&gt; (4/45; 8.8%), &lt;em&gt;K. quasipneumoniae&lt;/em&gt; (3/45; 6.6%), &lt;em&gt;K. pneumoniae&lt;/em&gt; subsp. &lt;em&gt;similipneumoniae&lt;/em&gt; (2/45; 4.4%), &lt;em&gt;K. aerogenes&lt;/em&gt; (1/45; 2.2%)&lt;em&gt;,&lt;/em&gt; and &lt;em&gt;K. michiganensis&lt;/em&gt; (1/45; 2.2%). Considering all the sources, 57.8 % of the isolates resulted in resistant to trimethoprim-sulfamethoxazole, while a lower percentage of strains showed resistance to aztreonam (17.8%), cefpodoxime (17.8%), cefotaxime (22.2%), ceftazidime (22.2%), ciprofloxacin (24.4%), colistin (15.6%), gentamicin (8.9%), levofloxacin (2.2%) and tobramycin (11.1%). In general, resistant strains were detected more frequently in samples of human and animal origin than in those of food origin. A total of 17 distinct STs were identified, including 9 novel STs. The fluoroquinolones resistance was mainly attributed to the presence of gene &lt;em&gt;qnrS1&lt;/em&gt; (33%), while &lt;em&gt;strB&lt;/em&gt; (22%), &lt;em&gt;strA&lt;/em&gt; (17.7%), and &lt;em&gt;aac (6′)-Ibr-cr&lt;/em&gt; (11.1%) for aminoglycoside. β-lactam resistance genes &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;CTX−&lt;/sub&gt;&lt;sub&gt;M&lt;/sub&gt;&lt;em&gt;&lt;sub&gt;,&lt;/sub&gt; bla&lt;/em&gt;&lt;sub&gt;SHV&lt;/sub&gt;, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;TEM&lt;/sub&gt;, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;OXA&lt;/sub&gt;, and &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;OKP&lt;/sub&gt;-&lt;sub&gt;B&lt;/sub&gt; were considerably present in human (15/16; 93.7%) and animal (17/19; 89.5%) isolates. Among ESBL-encoding genes, &lt;em&gt;bla&lt;/em&gt;&lt;sub&gt;OXY-1&lt;/sub&gt; (1/10; 10%) was exclusively found in food samples. The emergence of antibiotic-resistant and novel lineages of &lt;em&gt;Klebsiella&lt;/em&gt; spp., with identical STs across human, animal, and food niches, indicates clonal expansion of high-risk strains. Surveillance and control of ESBL-producing &lt;em&gt;Enterobacteriaceae&lt;/em&gt; using the One Health approach are urgently needed in Northern Nigeria.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Importance&lt;/h3&gt;&lt;div&gt;. This study contributes to the understanding of the epidemiology of &lt;em&gt;Klebsiella&lt;/em&gt; spp. in Nigeria. The study highlights that even in regions with relatively limited use of last resort antibiotics, high-risk global clones are circulating. These findings, aligned with the One Health approach, and emphasize the close interconnection between human, animal, and food sources, which facilitates the spread of common clones. Given the continuous emergence of new resistance mechanisms in &lt;em&gt;Klebsiella&lt;/em&gt; spp., ongoing surveillance across Africa is war","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100458"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145018582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Di-(2-ethylhexyl) phthalate induces endometriosis by modulating IGF-1 m6A methylation via the intestinal Odoribacter–butyric acid axis in female rats","authors":"Hailing Yang ,&nbsp;Yanxiu Sha ,&nbsp;Chunmei Zhang ,&nbsp;Xiuying Li ,&nbsp;Yingying Xu ,&nbsp;Te Liu","doi":"10.1016/j.crmicr.2025.100474","DOIUrl":"10.1016/j.crmicr.2025.100474","url":null,"abstract":"<div><div>Endometriosis, a complex gynecological disorder characterized by aberrant growth of endometrial tissue outside the uterine cavity, poses a significant challenge to women's health. Emerging evidence implicates environmental pollutants, particularly di-(2-ethylhexyl) phthalate (DEHP), as potential contributors to endometriosis development. However, the precise molecular mechanisms underlying this effect remain poorly understood. Herein, we investigated the role of intestinal <em>Odoribacter</em>/butyric acid-mediated m6A methylation in METTL3/IGF-1 signaling in DEHP-induced epithelial-mesenchymal transition (EMT) and endometriosis in a rat model. Our study demonstrated that DEHP exposure alters the gut microbiota composition, leading to modulation of METTL3-mediated m6A modification in the insulin-like growth factor 1 (IGF-1) pathway. This modification enhances EMT in endometrial cells and promotes endometriotic lesion formation. We used a multi-layered approach, including 16S rRNA sequencing, targeted metabolomics, MeRIP-seq, quantitative polymerase chain reaction, western blotting, and immunohistochemistry, to elucidate the mechanistic role of intestinal <em>Odoribacter</em>/butyric acid pathway-mediated METTL3/IGF-1 m6A modification in DEHP-induced endometriosis. The results revealed a significant shift in microbial diversity and a corresponding increase in METTL3/IGF-1 m6A methylation in DEHP-exposed rats, which was directly linked to EMT markers such as E-cadherin and N-cadherin. Our findings reveal a novel gut microbiota-mediated mechanism by which DEHP exposure drives endometriosis via m6A methylation, providing valuable insights into the environmental and molecular basis of the disease. This study not only advances our understanding of the role of DEHP in endometriosis pathogenesis, but also suggests a putative intestinal <em>Odoribacter</em>–butyrate–METTL3/IGF-1 axis that may contribute to disease progression. However, these associations remain correlative, and causality requires further validation through functional experiments.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100474"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}