Current Research in Microbial Sciences最新文献_第2页

“Reflexions on the role, diversity, conservation and management of the genetic microbial resources in Agriculture”

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100365

María del Pilar Rodríguez Guzmán , Ismael Fernando Chávez Díaz , Lily Xochilt Zelaya Molina

引用次数: 0

Unearthing the burden of melioidosis in North India – an emerging threat in a non-endemic region

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100344

Shweta Raina , Disha Gautam , Rohit Kumar , Kavita Sisodia , Chiranjay Mukhopadhyay , Harpreet Kaur , Mohammed Ashiq , Rushika Saksena

{"title":"Unearthing the burden of melioidosis in North India – an emerging threat in a non-endemic region","authors":"Shweta Raina , Disha Gautam , Rohit Kumar , Kavita Sisodia , Chiranjay Mukhopadhyay , Harpreet Kaur , Mohammed Ashiq , Rushika Saksena","doi":"10.1016/j.crmicr.2025.100344","DOIUrl":"10.1016/j.crmicr.2025.100344","url":null,"abstract":"<div><div><em>Burkholderia pseudomallei (B. pseudomallei)</em> is the causative agent of the high-mortality disease called melioidosis. It is a severe infection that can be misdiagnosed due to variable presentation and low awareness among clinicians of the disease. It is endemic in India and well-described in southern and eastern coastal states. In the last decade, sporadic cases of melioidosis have been diagnosed in North Indian states, predominantly Rajasthan and Gujarat. The reported cases highlight the many risk factors for infection in this region that was not previously recognised as being endemic for melioidosis, including high prevalence of diabetes mellitus, alcoholism, and large rural population engaged in paddy cultivation. Climate change results in frequent flooding and waterlogging in urban areas, leading to exposure of soil harbouring <em>B. pseudomallei,</em> thus a contributing factor to the rise in cases in cities. As North India has not previously been considered an endemic region for melioidosis, wider awareness amongst clinicians and laboratorians is essential for early identification of symptoms, testing for <em>B. pseudomallei</em> in microbiology laboratories, and timely management of the disease to save lives lost to misdiagnosis. The present article describes various aspects of melioidosis in North India including diverse clinical manifestations, risk factors, and possible reasons for misdiagnosis and underreporting.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100344"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Irrigation water and soil chemistry shape fungal guilds in date palm soils, enhancing pathotroph abundance under saline groundwater irrigation

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100370

Subha Chandran , Dinesh Sanka Loganathachetti , Balamurugan Sadaiappan , Sanjay Swarup , Sunil Mundra

{"title":"Irrigation water and soil chemistry shape fungal guilds in date palm soils, enhancing pathotroph abundance under saline groundwater irrigation","authors":"Subha Chandran , Dinesh Sanka Loganathachetti , Balamurugan Sadaiappan , Sanjay Swarup , Sunil Mundra","doi":"10.1016/j.crmicr.2025.100370","DOIUrl":"10.1016/j.crmicr.2025.100370","url":null,"abstract":"<div><div>Saline groundwater irrigation is a predominant practice, especially in date palm (<em>Phoenix dactylifera L</em>.) farms in arid agroecosystems with scarce freshwater resources. Despite its economic importance, the influence of saline groundwater irrigation on bulk soil fungi remains overlooked. This study examined how saline groundwater irrigation affects fungal diversity, community structure, and assembly processes. Bulk soils from date palm farms with distinct irrigation sources (freshwater and saline groundwater), were analysed, and fungal community analyses were done using the internal transcribed spacer 2 (ITS2) sequencing. Soils irrigated with saline groundwater had a lower percentage (27%) of unique operational taxonomic units (OTUs) compared to freshwater (33.3%). Fungal richness negatively correlated with soil pH. Differences observed in overall and guild-specific fungal communities, with irrigation water electrical conductivity (EC) emerging as a pivotal factor distinguishing between the two irrigation sources. Notably, pathotrophs abundance was significant in soils irrigated with saline groundwater. Furthermore, the dominant pathotroph <em>Fusarium</em>, exhibited drift-based assembly process and was observed to be high under saline groundwater irrigation. Our study reveals that groundwater salinity reduces the number of unique OTUs and alters fungal communities at the overall and guild levels. This insight aids agricultural improvement in regions where saline groundwater is a predominant water source.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100370"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic wounds and adaptive Pseudomonas aeruginosa: A phenotypic and genotypic characterization

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100348

Kasandra Buchholtz , Rie Jønsson , Rasmus L. Marvig , Biljana Mojsoska , Karen Angeliki Krogfelt

引用次数: 0

Heptad repeat 1-derived N peptide inhibitors improve broad-spectrum anti-HIV-1 activity

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100364

Chen Yuan , Jia-Ye Wang , Bu-Yi Wang , Yi-Lin Zhao , Yan Li , Di Li , Hong Ling , Min Zhuang

{"title":"Heptad repeat 1-derived N peptide inhibitors improve broad-spectrum anti-HIV-1 activity","authors":"Chen Yuan , Jia-Ye Wang , Bu-Yi Wang , Yi-Lin Zhao , Yan Li , Di Li , Hong Ling , Min Zhuang","doi":"10.1016/j.crmicr.2025.100364","DOIUrl":"10.1016/j.crmicr.2025.100364","url":null,"abstract":"<div><h3>Background</h3><div>HIV-1 N-peptide inhibitor (NPI) derived from N-terminal heptad-repeat region (HR1) of gp41 can target C-terminal heptad-repeat region (HR2) or the HR1 to interfere with the formation of endogenous six-helix bundle (6HB). However, the NPI is less active than the C-peptide inhibitor. In this study, we reported three HR1-derived NPIs designed by adding fusion peptide proximal region (FPPR) of gp41 or a trimeric motif MTQ into the N36 peptide and then evaluated their anti-HIV-1 activities.</div></div><div><h3>Methods</h3><div>Molecular modeling was performed using Swiss Model. The inhibitory activity of NPIs on HIV-1 was assessed by Env-pseudovirus infection assays and cell-cell fusion assays. Interaction between NPIs and HR2 peptides was evaluated by circular dichroism and Native PAGE.</div></div><div><h3>Results</h3><div>The three newly designed NPIs, FPPR-N36, MTQ-N36, and MTQ-FPPR-N36, exhibited higher anti-HIV-1 activity than N36. The stability of the coiled-coil core formed by three designed NPIs or the 6HB formed by C34 and these NPIs were significantly higher than those of corresponding monomer N36 or isoleucine zipper-engineered trimeric N36 (IZN36). The 50 % inhibitory concentrations (IC<sub>50</sub>) of MTQ-N36 against HIV-1 infection were at a nanomolar level, lower than those of other tested NPIs. The FPPR-N36 could also inhibit infection of HIV-1 strains that were resistant to N36 and IZN36.</div></div><div><h3>Conclusions</h3><div>The three newly designed NPIs had inhibitory activity against HIV-1 infection. Among them, MTQ-N36 exhibited a higher potential to inhibit HIV-1 entry than other peptides, and FPPR-N36 might be a promising candidate NPI for suppressing HIV-1 strains that are resistant to conventional NPIs.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100364"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion of maltose/sucrose related transporters in Ascomycetes and their association with corresponding disaccharide utilization

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100368

Li Xu , Alessia Manassero , Berend Snel , Ronald P. de Vries , Mao Peng

{"title":"Expansion of maltose/sucrose related transporters in Ascomycetes and their association with corresponding disaccharide utilization","authors":"Li Xu , Alessia Manassero , Berend Snel , Ronald P. de Vries , Mao Peng","doi":"10.1016/j.crmicr.2025.100368","DOIUrl":"10.1016/j.crmicr.2025.100368","url":null,"abstract":"<div><div>Sugar transporters (STs) play a crucial role in mediating sugar uptake in fungi and have been increasingly studied due to their important biological roles and industrial potential. In this study, we performed a comprehensive phylogenetic analysis of STs across the fungal kingdom, including species from Ascomycota, Basidiomycota, Mucoromycota and Zoopagomycota. The results revealed a striking diversity of STs among these fungal phyla with respect to their genomic content and predicted sugar specificity. Particularly, we identified a remarkable expansion of maltose/sucrose STs and a strong co-expansion of intracellular α-1,4-glucosidases and invertases in Ascomycota compared to other fungal phyla. In addition, growth profiles support that the utilization of maltose and sucrose across a diverse set of fungi is roughly determined by the presence of both corresponding STs and functionally related hydrolases. This study enhances our understanding of evolutional diversity of fungal STs and provides new insights into metabolic engineering of fungi towards more efficient conversion of plant-derived sugars for relevant industrial applications.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100368"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the efficacy of an antimicrobial peptide in the context of cystic fibrosis airways

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100367

Albane Jouault , Inès Jeguirim , Inès Ben Hadj Kaddour , Lhousseine Touqui

{"title":"Assessment of the efficacy of an antimicrobial peptide in the context of cystic fibrosis airways","authors":"Albane Jouault , Inès Jeguirim , Inès Ben Hadj Kaddour , Lhousseine Touqui","doi":"10.1016/j.crmicr.2025.100367","DOIUrl":"10.1016/j.crmicr.2025.100367","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) offer a promising alternative to control airway infections with multi-resistant bacteria, such as methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), which commonly infects patients with cystic fibrosis (CF). However, the behavior of AMPs in the CF context has yet to be fully elucidated. CF airways produce large amounts of proteases and viscous mucus (sputum), which may affect the efficacy of AMPs. The present work aimed to determine whether CF conditions affect the bactericidal efficacy of CAMA, a promising AMP known to kill clinical MRSA strains efficiently. Using a killing assay, we quantified CAMA bactericidal activity on a CF clinical MRSA strain in the presence of several compounds of CF airways, including sputum and bronchial epithelial cells (BECs). Our results indicate that CF sputum impairs the bactericidal efficacy of CAMA. Similar results were observed when CAMA was incubated with an artificial sputum medium (ASM). When used separately, sputum components (DNA, lipids, and mucins) reproduced the inhibitory effects of ASM. Additionally, the bactericidal efficacy of CAMA was also slightly altered when planktonic <em>S. aureus</em> strains were co-cultured with CF BECs. However, CAMA was not active against <em>S. aureus</em> cultured on BEC in biofilm mode, characteristic of chronic infections in CF patients. These findings suggest that although CAMA represents a promising tool to treat MRSA strains, the CF environment may impair the efficacy of this AMP. Identifying strategies to protect AMPs from the deleterious effects of CF sputum is a key priority.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100367"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Salinity tolerance in resting cysts of colpodid ciliates: Comparative transcriptomics analysis and chemical analysis of cyst walls to investigate their tolerance capability 纤毛虫休眠囊的耐盐性：通过比较转录组学分析和囊壁化学分析研究囊虫的耐盐能力

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100371

Ryota Saito , Hiroki Yamanobe , Kazuma Yabuki , Tomohiro Suzuki , Takeru Saito , Shuntaro Hakozaki , Manfred Wanner , Ryota Koizumi , Tatsuya Sakai , Maribet Gamboa , Toshihiko Tanaka , Akiko Ono , Hoa Thanh Nguyen , Yuta Saito , Tetsuya Aoyama , Katsuhiko Kojima , Futoshi Suizu , Kozo Watanabe , Yoichiro Sogame

{"title":"Salinity tolerance in resting cysts of colpodid ciliates: Comparative transcriptomics analysis and chemical analysis of cyst walls to investigate their tolerance capability","authors":"Ryota Saito , Hiroki Yamanobe , Kazuma Yabuki , Tomohiro Suzuki , Takeru Saito , Shuntaro Hakozaki , Manfred Wanner , Ryota Koizumi , Tatsuya Sakai , Maribet Gamboa , Toshihiko Tanaka , Akiko Ono , Hoa Thanh Nguyen , Yuta Saito , Tetsuya Aoyama , Katsuhiko Kojima , Futoshi Suizu , Kozo Watanabe , Yoichiro Sogame","doi":"10.1016/j.crmicr.2025.100371","DOIUrl":"10.1016/j.crmicr.2025.100371","url":null,"abstract":"<div><div>The formation of resting cysts is a strategy for survival in unfavorable environments by single cell organisms such as protists. Here, we show that <em>Colpoda</em> resting cysts have high salinity tolerance and investigate the changes in gene expression that underpin this effect. <em>Colpoda</em> resting cysts can tolerate saline conditions up to 3.5 % NaCl. A comparative transcriptome analysis of vegetative cells and resting cysts showed that the relative levels of expression of genes associated with membrane function increased in resting cysts. These changes in gene expression suggest that reconstruction of the plasma membrane is associated with salinity tolerance. The resting cyst forms cyst-specific cellular structure known as the cyst wall. The outer shell-like layer, called the ectocyst, while the inner multiple layers, known as the endocyst. The chemical analysis showed ectocyst contains chitin and endocyst contains several proteins. These structures can protect cells by acting as a biological armor or protective materials. The results of this study offer a possible scenario in which salinity tolerance enables the widespread dispersal of protists.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100371"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143619376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flocculation Mechanisms in Brettanomyces bruxellensis: Influence of ethanol and sulfur dioxide on FLO gene expression

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2025.100372

Alessandra Di Canito, Roberto Foschino, Ileana Vigentini

{"title":"Flocculation Mechanisms in Brettanomyces bruxellensis: Influence of ethanol and sulfur dioxide on FLO gene expression","authors":"Alessandra Di Canito, Roberto Foschino, Ileana Vigentini","doi":"10.1016/j.crmicr.2025.100372","DOIUrl":"10.1016/j.crmicr.2025.100372","url":null,"abstract":"<div><div>The mechanisms underlying flocculation in <em>Brettanomyces bruxellensis</em>, unlike the well-characterized <em>FLO</em>-family gene regulation in <em>Saccharomyces cerevisiae</em>, remain largely unexplored. This study investigates the flocculant phenotypes of 99 <em>B. bruxellensis</em> strains, revealing that only a minority exhibits this clumping behavior and confirms its strain-dependent attitude. Focusing on two strains, CBS2499 (flocculant) and UMY321 (non-flocculant), genetic analysis uncovered polymorphisms and distinct allelic heterozygosity in the <em>FLO1</em> and <em>FLO11</em> genes, potentially linked to the phenotypic differences. To further examine these traits, Response Surface Methodology (RSM) was used to simulate oenological conditions, testing the impact of pH, ethanol, and sulfur dioxide (SO₂) levels on flocculation and gene expression. The findings revealed that environmental stressors, especially ethanol and SO₂, significantly increase the expression of <em>FLO1</em> and <em>FLO11</em> in CBS2499, indicating a regulatory role in flocculation under stress. These insights broaden our understanding of stress adaptation in <em>B. bruxellensis</em>, especially its survival strategies in wine environments. By elucidating factors influencing flocculation, this study contributes valuable knowledge for managing <em>B. bruxellensis</em> spoilage, potentially aiding in the development of targeted approaches to reduce its impact on wine quality.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100372"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-microbe interactions: PGPM as microbial inoculants/biofertilizers for sustaining crop productivity and soil fertility 植物与微生物的相互作用：PGPM作为维持作物生产力和土壤肥力的微生物接种剂/生物肥料。

IF 4.8

Current Research in Microbial Sciences Pub Date : 2025-01-01 DOI: 10.1016/j.crmicr.2024.100333

Bibek Laishram , Okram Ricky Devi , Rinjumoni Dutta , T. Senthilkumar , Girish Goyal , Dinesh Kumar Paliwal , Narinder Panotra , Akhtar Rasool

{"title":"Plant-microbe interactions: PGPM as microbial inoculants/biofertilizers for sustaining crop productivity and soil fertility","authors":"Bibek Laishram , Okram Ricky Devi , Rinjumoni Dutta , T. Senthilkumar , Girish Goyal , Dinesh Kumar Paliwal , Narinder Panotra , Akhtar Rasool","doi":"10.1016/j.crmicr.2024.100333","DOIUrl":"10.1016/j.crmicr.2024.100333","url":null,"abstract":"<div><div>Plant-microbe interactions play pivotal roles in sustaining crop productivity and soil fertility, offering promising avenues for sustainable agricultural practices. This review paper explores the multifaceted interactions between plants and various microorganisms, highlighting their significance in enhancing crop productivity, combating pathogens, and promoting soil health. Understanding these interactions is crucial for harnessing their potential in agricultural systems to address challenges such as food security and environmental sustainability. Therefore, the introduction of beneficial microbes into agricultural ecosystems by bio-augmentation reduces the negative effects of intensive, non-sustainable agriculture on the environment, society, and economy, into the mechanisms underlying the application of plant growth promoting microbes as microbial inoculants/biofertilizers; their interactions, the factors influencing their dynamics, and the implications for agricultural practices, emerging technologies and strategies that leverage plant-microbe interactions for improving crop yields, soil fertility, and overall agricultural sustainability.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100333"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11743900/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0