Current Research in Microbial Sciences最新文献

{"title":"Arbuscular mycorrhizal interaction associated with a botanical garden in the tropics of Mexico","authors":"José Alberto Gío-Trujillo,&nbsp;Carlos J. Alvarado-López","doi":"10.1016/j.crmicr.2024.100320","DOIUrl":"10.1016/j.crmicr.2024.100320","url":null,"abstract":"<div><div>Botanical gardens, areas for vegetation conservation, have become important reservoirs of beneficial soil microbiota, mainly as a source of microbial inoculum for agricultural purposes. Shrubby mycorrhizal fungi (AMF), an important genetic resource of tropical soils, have a high potential for agricultural production, generally used as inoculant medium that provides better yield, productivity and physiological response to crops. This research study explores the presence of AMF in a botanical garden, composed of four areas: cactarium collection, epiphytes and ornamental collection, tropical forest area and coastal zone. Each area is composed of plants representative of its ecosystem. For the study, a random systematic model was used, with nine samples per site at a depth of 20 cm. A physicochemical characterization of the soils was developed. The extraction of AMF spores was carried out by wet sieving and centrifugation in 60% sucrose. The spores were identified by taxon. The results indicate a total of 379 AMF spores identified in the study area. The highest spore incidence was recorded in the tropical forest area with a total of 161 (53.67±5.51) spores extracted, followed by the coastal zone and cactus collection area with 78 (26.00±9.64) and 73 (24.33±4.73) spores in total. In the study two taxa were identified, Glomeraceae and Gigasporaceae. The taxon Glomeraceae is also considered to be the most representative (highest abundance and frequency) of the study. In conclusion, botanical gardens, by their extructure (plant composition) and management can be taken into account as important ecosystems reservoirs and source of viable microbial genetic material for the bioprospecting of beneficial soil genotic resources (rhizophiles and endophylls) for use in modern agriculture and sustainable food production systems.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100320"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142721803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging virucidal potential of an anti-microbial coating agent to mitigate fomite transmission of respiratory viruses","authors":"Bommana Chanakya ,&nbsp;Kavitha Karunakaran ,&nbsp;Oliver Christy Dsa ,&nbsp;Anil Prataprai Sanghvi ,&nbsp;Chiranjay Mukhopadhyay ,&nbsp;Piya Paul Mudgal","doi":"10.1016/j.crmicr.2024.100261","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100261","url":null,"abstract":"<div><p>In the wake of the COVID-19 pandemic, respiratory tract infections have emerged as a significant global threat, yet their impact on public health was previously underappreciated. This study investigated the antiviral efficacy of the nano-coating agent BARRIER90, composed of silicon-quaternary ammonium compound and a naturally derived biopolymer, against three distinct respiratory viruses: Influenza A (H1N1), Adenovirus Type 1, and Enterovirus-Coxsackie B1. BARRIER90 exhibited robust and sustained virucidal activity, persisting up to 90 days post-coating, against the enveloped virus, Influenza A, with significant reduction in viral plaques. Contrastingly, its efficacy against non-enveloped viruses revealed transient activity against Enterovirus-Coxsackie B1, with almost no antiviral activity observed against Adenovirus Type 1. These findings indicate the potential of antimicrobial coatings in mitigating viral transmission through contaminated surfaces (fomites), which harbour pathogenic viruses for longer periods. Antimicrobial coatings may facilitate infection control in various settings, including healthcare facilities and shared workspaces.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100261"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000439/pdfft?md5=b03a15078dbb5844912bf479e2b6bdd6&pid=1-s2.0-S2666517424000439-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141606664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial contributions to sustainable paddy straw utilization for economic gain and environmental conservation","authors":"Baljeet Singh Saharan ,&nbsp;Deepika Dhanda ,&nbsp;Neelam Kumari Mandal ,&nbsp;Ramesh Kumar ,&nbsp;Deepansh Sharma ,&nbsp;Pardeep Kumar Sadh ,&nbsp;Dilfuza Jabborova ,&nbsp;Joginder Singh Duhan","doi":"10.1016/j.crmicr.2024.100264","DOIUrl":"10.1016/j.crmicr.2024.100264","url":null,"abstract":"<div><p>Paddy straw is a versatile and valuable resource with multifaceted benefits for nutrient cycling, soil health, and climate mitigation. Its role as a rich nutrient source and organic matter significantly enhances soil vitality while improving soil structure and moisture retention. The impact of paddy straw extends beyond traditional agricultural benefits, encompassing the promotion of microbial activity, erosion control, and carbon sequestration, highlighting its crucial role in maintaining ecological balance. Furthermore, the potential of paddy straw in bioenergy is explored, encompassing its conversion into biogas, biofuels, and thermal energy. The inherent characteristics of paddy straw, including its high cellulose, hemicellulose, and lignin content, position it as a viable candidate for bioenergy production through innovative processes like pyrolysis, gasification, anaerobic digestion, and combustion. Recent research has uncovered state-of-the-art techniques and innovative technologies capable of converting paddy straw into valuable products, including sugar, ethanol, paper, and fiber, broadening its potential applications. This paper aims to underscore the possibilities for value creation through paddy straw, emphasizing its potential use in bioenergy, bio-products, and other environmental applications. Therefore, by recognizing and harnessing the value of paddy straw, we can advocate for sustainable farming practices, reduce waste, and pave the way for a resource-efficient circular economy. Incorporating paddy straw utilization into agricultural systems can pave the way for enhanced resource efficiency and a more sustainable circular economy.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100264"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000464/pdfft?md5=85663c8ca88d7bb242da35d1d00533d2&pid=1-s2.0-S2666517424000464-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141846682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into population adaptation and biodiversity of lactic acid bacteria in challenged date palm leaves silaging, using MALDI–TOF MS","authors":"Muhammad Zaid Jawaid,&nbsp;Mohammad Yousaf Ashfaq,&nbsp;Mohammad Al-Ghouti,&nbsp;Nabil Zouari","doi":"10.1016/j.crmicr.2024.100235","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100235","url":null,"abstract":"<div><p>The study focused on isolating indigenous Qatari lactic acid bacteria (LAB) from various challenged date palm tree leaf silages to construct a comprehensive strain collection, useful to study the diversity of these strains following their adaptation to the uncommon silage. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI–TOF MS) was employed for strain identification and differentiation. The diversity of LAB populations and strains was assessed through principal component analysis (PCA) and dendrogram analyses. A total of 88 LAB isolates were obtained from silages of fresh palm leaves, silage of mixed leaves and dairy feed, along with fresh palm tree leaves, and dairy feed, adapted to local harsh environments. These isolates were categorized according to the new classification of 2020, belonging to genera of <em>Pediococcus, Lactiplantibacillus plantarum, Lacticaseibacillus paracasei, Companilactobacillus farciminis, Limosilactobacillus oris, Limosilactobacillus vaginalis, Lactiplantibacillus pentosus</em> and <em>Lactobacillus johnsonii. Pediococcus</em> was the most prevalent genus, falling mostly within the species <em>Pediococcus lolii</em>. MALDI–TOF MS protein profiles, PCA, and dendrogram analyses successfully grouped the LAB isolates into five distinctive clusters based on the protein's similarities. The high diversity of the indigenous LAB in spontaneous palm leaf silages demonstrated their adaptation and mutualistic interactions, forming robust consortia that ensure the quality of the silage. The straightforward, quick, and accurate identification of LAB in this silage using MALDI–TOF MS presents a valuable approach for formulating LAB consortia for silaging harsh agricultural by-products.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100235"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000178/pdfft?md5=42956d076b3b4bab2a3a1d2fac807a90&pid=1-s2.0-S2666517424000178-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140605265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brewers’ spent grain as fish feed ingredient: Evaluation of bio-safety and analysis of its impact on gut bacteria of Cirrhinus reba by 16S Metagenomic sequencing","authors":"Sourav Chattaraj ,&nbsp;Debasis Mitra ,&nbsp;Manasi Chattaraj ,&nbsp;Arindam Ganguly ,&nbsp;Hrudayanath Thatoi ,&nbsp;Pradeep K. Das Mohapatra","doi":"10.1016/j.crmicr.2024.100286","DOIUrl":"10.1016/j.crmicr.2024.100286","url":null,"abstract":"<div><div>A comprehensive eight week feeding trial was conducted to investigate the potential of brewers' spent grain (BSG) as a sustainable fish feed ingredient. The study assessed both the biosafety of BSG and its impact on the gut microbiome of <em>Cirrhinus reba</em>, utilizing advanced 16S metagenomic sequencing techniques to analyze the composition and diversity of gut bacteria. A total of 90 healthy <em>C. reba</em> juveniles (average weight: 12 ± 1 g) were divided into two dietary groups [for control (C), for BSG meal (tB)] in triplicates. Feed prepared with conventional ingredients was used to feed the control group (C). The group tB was fed with BSG meal. After the feeding trial, the fish in tB group showed significantly higher (<em>p</em> &lt; 0.05) growth parameters as compared to the control group. The results of bio-safety assessment indicated the absence of any pathological symptoms in the BSG meal fed carps. The fish in tB group didn't show any histopathological abnormality. Fish fed the Brewers' Spent Grain exhibited significantly elevated serum biochemical parameters, including alanine transaminase (ALT) and aspartate transaminase (AST), compared to the control group (<em>p</em> &lt; 0.05). 16S Metagenomic sequencing of the fish gut microbiota provides insights into how BSG inclusion affects microbial diversity and composition within the digestive tract of <em>C. reba</em>. The analysis revealed the existence of 240 and 250 diverse bacterial genera in the gastrointestinal tract (GIT) of <em>C. reba</em> in dietary groups C and tB respectively. Importantly, the study found the gut of fish in tB group to be dominated by different beneficial genus including <em>Bacillus, Lactobacillus, Bifidobacterium, Paenibacillus,</em> and <em>Lysinibacillus</em>. Feeding <em>C. reba</em> with BSG meal significantly increased the alpha diversity of the gastrointestinal microbiota, as evidenced by elevated Chao 1 estimator and Shannon index values compared to the control diet (<em>p</em> &lt; 0.05). This study provides comprehensive evidence for the bio-safety of BSG as a sustainable feed ingredient in aquaculture, demonstrating its potential to support healthy fish growth and development. Moreover, the prebiotic potential of BSG in fish has also been highlighted.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100286"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling growth-promoting potential of plant beneficial actinobacteria on tropical bryophytes","authors":"Mathurin Meethangdee ,&nbsp;Wasu Pathom-aree","doi":"10.1016/j.crmicr.2024.100284","DOIUrl":"10.1016/j.crmicr.2024.100284","url":null,"abstract":"<div><div>Bryophytes are non-vascular plants with dominant gametophyte stage that play vital ecological roles in natural ecosystems. Unfortunately, their populations are currently in decline due to habitat destruction and various anthropogenic activities. The conservation efforts for bryophytes are hampered by their slow growth rates. This study aims to investigate the potential of actinobacteria to promote the growth of bryophytes. In this study, three plant growth-promoting actinobacteria, <em>Dermacoccus abyssi</em> MT1.1^T, <em>Micromonospora chalcea</em> CMU55-4 and <em>Streptomyces thermocarboxydus</em> S3 were cultured in International <em>Streptomyces</em> Project medium 2 (ISP2) broth to obtain culture filtrates containing bioactive compounds for enhancing the growth of two bryophyte species, <em>Physcomotrium sphaericum</em> (C. Ludw.) Fürnr and <em>Sphagnum cuspidatulum</em> C. Müll. Interestingly, the incorporation of actinobacterial culture filtrates into 1/16 Murashige and Skoog (MS) medium yielded superior growth performance of <em>P. sphaericum</em> (C. Ludw.) Fürnr and <em>S. cuspidatulum</em> C. Müll, as observed from the thallus height, fresh weight, total chlorophyll contents, and total carotenoid contents compared to control groups. In addition, the inoculation of <em>M. chalcea</em> CMU55-4 on <em>S. cuspidatulum</em> C. Müll grown in sterile peat moss demonstrated the highest values for thallus height, fresh weight, dry weight, total chlorophyll content, and total carotenoid content. All actinobacteria successfully colonized the moss seedlings without any observable negative impacts, indicating beneficial interactions between actinobacteria and bryophytes. This research sheds light on the potential of harnessing plant beneficial actinobacteria to enhance the growth of bryophytes for conservation purposes.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100284"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptolysin S induces proinflammatory cytokine expression in calcium ion-influx-dependent manner","authors":"Yugo Yamamori ,&nbsp;Rina Shirai ,&nbsp;Kazuto Ohkura ,&nbsp;Hideaki Nagamune ,&nbsp;Toshifumi Tomoyasu ,&nbsp;Atsushi Tabata","doi":"10.1016/j.crmicr.2024.100265","DOIUrl":"10.1016/j.crmicr.2024.100265","url":null,"abstract":"<div><p>Anginosus group streptococci (AGS) are opportunistic pathogens that reside in the human oral cavity. The β-hemolytic strains of <em>Streptococcus anginosus</em> subsp. <em>anginosus</em> (SAA) produce streptolysin S (SLS), a streptococcal peptide hemolysin. In recent clinical scenarios, AGS, including this species, have frequently been isolated from infections and disorders beyond those in the oral cavity. Consequently, investigating this situation will reveal the potential pathogenicity of AGS to ectopic infections in humans. However, the precise mechanism underlying the cellular response induced by secreted SLS and its relevance to the pathogenicity of AGS strains remain largely unknown. This study aims to elucidate the mechanism underlying the host cellular response of the human acute monocytic leukemia cell line THP-1 to secreted SLS. In THP-1 cells incubated with the culture supernatant of β-hemolytic SAA containing SLS as the sole cytotoxic factor, increased Ca²⁺ influx and elevated expression of proinflammatory cytokines were observed. Significantly reduced expression of SLS-dependent upregulated cytokine genes under Ca²⁺-chelating conditions suggests that Ca²⁺ influx triggers SLS-dependent cellular responses. Furthermore, SLS-dependent enhanced expression of IL-8 was also implicated in the activation of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (MAPK) signaling pathways. The findings presented in this study are crucial for a comprehensive understanding of the real pathogenicity of SLS-producing β-hemolytic AGS in the latest clinical situations.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100265"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000476/pdfft?md5=cb45c45e4c295bc59d776d6585a4f4dc&pid=1-s2.0-S2666517424000476-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valorisation of raw mango pickle industry waste into antimicrobial agent against postharvest fungal pathogens","authors":"Gouthami Shivaswamy ,&nbsp;Shalini Gaur Rudra ,&nbsp;Lham Dorjee ,&nbsp;Aditi Kundu ,&nbsp;Robin Gogoi ,&nbsp;Anupama Singh","doi":"10.1016/j.crmicr.2024.100243","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100243","url":null,"abstract":"<div><p>In mango pickle industry, a significant quantity of mango seed kernels is discarded as solid wastes. These seed kernels can be an ideal source for obtaining extracts rich in bioactive polyphenolic compounds with good antioxidant properties. The potential of mango kernel phenolic extract (MKPE) was investigated as a natural and effective antimicrobial agent for controlling major postharvest fungal pathogen infections, a significant threat to global food supply chains. Fungal pathogens contribute to the deterioration of fruits, vegetables, and grains during storage and transportation, leading to economic losses and compromised food safety. MKPE was obtained from pickling variety 'Ramkela' raw mango kernels, and its phenolic composition was characterized using LC–MS. The <em>in vitro</em> antifungal activity of MKPE against <em>Botrytis cinerea, Colletotrichum gloeosporoides</em>, and <em>Rhizopus stolonifer</em> was evaluated in vitro. A concentration-dependent inhibition of fungal radial growth against all three pathogens was observed, exhibiting the potential of MKPE as a valuable natural resource for addressing postharvest losses caused by fungal pathogens. The extraction process yielded a total phenolic content of 2128 mg GAE/100 g. Major polyphenolic bioactive compounds present were mangiferin, quercetin, and rhamnetin. The <em>in-vitro</em> antimicrobial assay showed reduction in the radial growth and inhibition percent of the pathogens. EC₅₀ values of MKPE for <em>B. cineria, C. gloeosporoides</em>, and <em>R. stolonifer</em> was found to 364.17, 963.8 and 926 ppm, respectively. Our results demonstrate an economical, sustainable, and eco-friendly approach to manage post-harvest diseases rendered by fungi using mango MKPE from pickling industry waste.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100243"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000257/pdfft?md5=2276d8e8e7ce495850d02341830871f9&pid=1-s2.0-S2666517424000257-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141243896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of biodegradable food packaging from mango peel via enzymatic hydrolysis and polyhydroxyalkanoates synthesis: A review on microbial intervention","authors":"Vinay Kumar Pandey ,&nbsp;Zaryab Shafi ,&nbsp;Anjali Tripathi ,&nbsp;Gurmeet Singh ,&nbsp;Rahul Singh ,&nbsp;Sarvesh Rustagi","doi":"10.1016/j.crmicr.2024.100292","DOIUrl":"10.1016/j.crmicr.2024.100292","url":null,"abstract":"<div><div>The rising environmental problem of plastic packaging waste has led to the development of sustainable alternatives, particularly for food packaging. Polyhydroxyalkanoates (PHAs) are biodegradable, thermoplastic polyesters. They are employed in the production of various products, including packaging films. The bio-based nature and appropriate features of PHAs, similar to conventional synthetic plastics, have garnered significant attention from researchers and industries. The current study aimed to produce biodegradable food packaging using mango peel (a major agricultural waste) with enzymatic hydrolysis and PHAs synthesis. Mango peel is the hub for macro-and micronutrients, including phytochemicals. The process includes an enzymatic hydrolysis step that converts complex carbohydrates into simple sugars using mango peel as a substrate. The produced sugars are used as raw materials for bacteria to synthesize PHAs, which are a class of biodegradable polymers produced by these microorganisms that can serve as packaging materials in the food industry. To solve environmental problems and increase the utilization of agricultural by-products, this review presents a practical method for producing food packaging that is environmentally friendly.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100292"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Novel Drug Targets and Drug Design for Bordetella pertussis: A Subtractive Proteomics Approach","authors":"Md. Nazmul Islam Bappy ,&nbsp;Foeaz Ahmed ,&nbsp;Tahera Lasker ,&nbsp;Emran Hossain Sajib ,&nbsp;Md. Shariful Islam","doi":"10.1016/j.crmicr.2024.100291","DOIUrl":"10.1016/j.crmicr.2024.100291","url":null,"abstract":"<div><div><em>Bordetella pertussis</em> causes whooping cough in humans that spreads directly from individual to individual mainly by aerosolized respiratory droplets. Nowadays, it gained the attention of scientific community because it has already been reemerged as one of the major public health threats despite widespread vaccination efforts. Moreover, the growing antibiotic resistance has made it difficult to combat this pathogen with currently available antibiotics. Consequently, screening drug targets and discovering drugs against unique proteins of the pathogen could be a promising alternative. With this view, 3,359 proteins of <em>B. pertussis</em> were screened in silico to identify non-duplicate proteins crucial for survival of the bacteria, non-homologous to humans, involved in unique metabolic pathways of the pathogen, and conserved among various bacterial strains. Among these, Chemotaxis protein Mota, Chromosomal replication initiator protein DnaA, Short-chain fatty acids transporter, [protein-PII] uridylyltransferase, Type III secretion protein V, Potassium-transporting ATPase potassium-binding subunit, N-acetylmuramoyl-L-alanine amidase, and RNA polymerase sigma-54 factor fulfilled these criteria. These proteins were further analyzed for qualitative characteristics such as virulence properties and associations with antibiotic resistance, etc. In addition, plant metabolites were screened against these unique proteins utilizing molecular docking to discover putative drugs against them. Four metabolites exhibited superior binding affinity and favorable ADME (Adsorption, distribution, metabolism, and excretion) properties which can further be tested in vivo.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100291"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}