Current Research in Microbial Sciences最新文献

{"title":"Analysis of novel bacterial metagenome-assembled genomes from lignin-degrading microbial consortia","authors":"Vitória Pinheiro Balestrini ,&nbsp;Otávio Henrique Bezerra Pinto ,&nbsp;Blake A. Simmons ,&nbsp;John M. Gladden ,&nbsp;Ricardo Henrique Krüger ,&nbsp;Betania Ferraz Quirino","doi":"10.1016/j.crmicr.2024.100302","DOIUrl":"10.1016/j.crmicr.2024.100302","url":null,"abstract":"<div><div>Despite recent progress, bacterial degradation of lignin is not completely understood. To address the mechanisms that bacteria from unknown taxonomic groups use to perform lignin-monomer degradation, functional analysis of bacterial metagenome-assembled genomes from soil-derived consortia enriched for microorganisms capable of degrading lignin was performed. A total of 232 metagenome-assembled genomes were recovered. After applying quality criteria of at least 70 % genome completeness and contamination less than or equal to 10 %, 39 genomes were obtained. From these, a total of 14 genomes from bacteria of unknown classification at lower taxonomic levels (i.e., only classified to the order level or higher) were chosen for further functional analysis. A global analysis of the potential ecological functions of these bacteria was performed, followed by a detailed analysis of monolignol degradation pathways. The phylum with the highest number of genomes was Proteobacteria. The genomes presented functions consistent with soil-derived bacteria, like denitrification, with different metabolic capacities related to the sulfur, chlorine, arsenic and carbon cycles, in addition to the degradation of plant cell wall components like cellulose, hemicellulose, and lignin. The Sphingomonadales_OP 08 genome showed the greatest potential to degrade cellulose and hemicellulose, although it does not appear to be able to degrade lignin. The Actinobacteria_BY 70 genome presented the highest number of enzymes and pathways related to the degradation of monolignols; furthermore, it showed the greatest potential for aromatic ring breakage by different fission pathways. The genomes of the two Actinobacteria showed the caffeic acid pathway, an important phenolic compound presenting several biological properties, such as antimicrobial and antioxidant. To our knowledge, this is the first time this pathway has been reported in this class of bacteria.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100302"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142586830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is it time to re-evaluate exposure risks to quaternary ammonium compounds as disinfectants?","authors":"Jason A. Tetro ,&nbsp;Faraz A. Alderson ,&nbsp;Syed A. Sattar","doi":"10.1016/j.crmicr.2024.100258","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100258","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100258"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000403/pdfft?md5=28fa56f50989c7c932ecd98ac94404c8&pid=1-s2.0-S2666517424000403-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141582569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human cytomegalovirus and neonatal infection","authors":"Shelley M. Lawrence","doi":"10.1016/j.crmicr.2024.100257","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100257","url":null,"abstract":"<div><p>Human cytomegalovirus is an ancient virus that has co-evolved with humans. It establishes a life-long infection in suspectable individuals for which there is no vaccination or cure. The virus can be transmitted to a developing fetus in seropositive pregnant women, and it is the leading cause of congenital infectious disease. While the majority of infected infants remain asymptomatic at birth, congenital cytomegalovirus infection can lead to substantial long-term neurodevelopmental impairments in survivors, resulting in considerable economic and social hardships. Recent discoveries regarding cytomegalovirus pathophysiology and viral replication cycles might enable the development of innovative diagnostics and therapeutics, including an effective vaccine. This Review will detail our understanding of human cytomegalovirus infection, with an in-depth discussion regarding the viral genome and transcriptome that contributes to its pathophysiology. The neonate's clinical course will also be highlighted, including maternal and neonatal testing, treatment recommendations, and long-term outcomes.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100257"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000397/pdfft?md5=ac750b4fff9e26378ae946408935652e&pid=1-s2.0-S2666517424000397-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141583042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An updated view of bacterial endophytes as antimicrobial agents against plant and human pathogens","authors":"Lalhmangaihmawia Hnamte ,&nbsp;Vanlallawmzuali ,&nbsp;Ajay Kumar ,&nbsp;Mukesh Kumar Yadav ,&nbsp;Zothanpuia ,&nbsp;Prashant Kumar Singh","doi":"10.1016/j.crmicr.2024.100241","DOIUrl":"10.1016/j.crmicr.2024.100241","url":null,"abstract":"<div><p>Bacterial endophytes are a crucial component of the phytomicrobiome, playing an essential role in agriculture and industries. Endophytes are a rich source of bioactive compounds, serving as natural antibiotics that can be effective in combating antibiotic resistance in pathogens. These bacteria interact with host plants through various processes such as quorum sensing, chemotaxis, antibiosis, and enzymatic activity. The current paper focuses on how plants benefit extensively from endophytic bacteria and their symbiotic relationship in which the microbes enhance plant growth, nitrogen fixation, increase nutrient uptake, improve defense mechanisms, and act as antimicrobial agents against pathogens. Moreover, it highlights some of the bioactive compounds produced by endophytes.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100241"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000233/pdfft?md5=7c2fc8440cd23aa2ccf5a5ee1423eff9&pid=1-s2.0-S2666517424000233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141134688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in groundwater and surface water bacterial communities under disinfection processes: Chlorination, ozonization, photo-fenton and ultraviolet radiation","authors":"Lucía Castaño-Henao ,&nbsp;David Felipe Garcia Mendez ,&nbsp;Siobhon Egan ,&nbsp;Janeth Sanabria","doi":"10.1016/j.crmicr.2024.100244","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100244","url":null,"abstract":"<div><p>Pathogenic bacteria, introduced in water sources through faecal contamination, have traditionally been investigated as individual species, leading to the establishment of microbial, sanitary, and environmental quality indicators. Recent advancements in our understanding of the microbiome and its intricate interactions within the human-microbiome-environment network advocate for a broader evaluation of the impact of disinfection on the entire microbial community. In this study, we conducted a comprehensive screening experiment involving four disinfection processes; ozone, ultraviolet radiation with wavelengths between 200 – 280 nm (UV-C), photo-Fenton, and chlorination, applied to two distinct water sources; surface (SW) and groundwater (GW). The cells that remained viable after treatment were recovered using Brain Heart Infusion (BHI) broth, and 16S rRNA gene sequencing was used for their identification. Our findings confirmed the presence of faecal contamination in the water sources and revealed distinct effects of each treatment on the recovered bacterial populations. The chlorination of groundwater samples likely had a greater impact on bacteria in a vegetative state than on spores. Consequently, this led to a higher abundance in the BHI cultures of sporulating bacteria such as <em>Bacillus</em> (increasing from 0.36 to 93.62 %), while ozonation led to an elevated recovery of <em>Pseudomonas</em> (increasing from 45.2 to 69.9 %). Conversely, in surface water, calcium hypochlorite and ozone treatments favored the selection of <em>Staphylococcus</em> and <em>Bacillus</em>, whose relative abundance in the cultures increased from 0 to 39.22 % and from 0.35 to 96.6 %, respectively. In groundwater, <em>Pseudomonas</em> was resistant to UV-C radiation and their relative abundance increased from 45.2 % to 93.56 %, while photo-Fenton was effective against this bacterial group decreasing its relative abundance to 0.46 %. However, other genera such as <em>Bacteroides, Aeromonas</em>, and <em>Citrobacter</em> seemed to be less injured by this disinfection process. BHI broth was successful in recovering various bacterial groups that exhibited resistance to sublethal water disinfection.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100244"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000269/pdfft?md5=f2384f79903b936120b2f57e26089d5c&pid=1-s2.0-S2666517424000269-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141314747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential gliding motility responses of Chryseobacterium sp. strain PMSZPI isolated from uranium ore deposit on hard and soft substrates","authors":"Lalitharashmi Yermunja ,&nbsp;Celin Acharya","doi":"10.1016/j.crmicr.2024.100309","DOIUrl":"10.1016/j.crmicr.2024.100309","url":null,"abstract":"<div><div>The <em>Bacteroidota</em> bacterium, <em>Chryseobacterium</em> sp. strain PMSZPI isolated from sub-surface soil of uranium ore deposit was shown to move on solid surfaces via gliding motility resulting in the formation of thin spreading colonies. In this study, we attempted to understand the influence of the surfaces, soft or hard/rigid, on the motility behaviour of PMSZPI cells. The computational tool T9GPred in combination with LC-MS/MS analysis established the presence of orthologs of vital gliding motility proteins in PMSZPI. We analyzed the single cell or population motility phenotypes of PMSZPI under spreading and non-spreading conditions. A low percentage of agar or soft agar (0.35 %) with low nutrient levels induced more active gliding motility in individual cells leading to increased colony spreading. Microscopic analyses indicated the self-assembly of the gliding cells into irregular edged or spherical microcolonies based on the agar concentration. Cells moved at a speed of 0.6 µm s^-1 on low-percentage gliding permissive agar (0.35 %) surface in contrast to significant inhibition of motility on rigid or hard agar (1.5 %) surface. RNA sequencing and real-time quantitative PCR (qPCR) analysis revealed increased expression of gliding motility genes under low agar conditions consistent with increased spreading behaviour. These findings provide the first glimpse into the gliding motility behaviour of a <em>Bacteroidota</em> bacterium from metal enriched environment that apparently could have implications on bacterial adaptation to changing surface environments.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100309"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerating the understanding of Aspergillus terreus: Epidemiology, physiology, immunology and advances","authors":"Raman Thakur ,&nbsp;Sonia Kumari Shishodia ,&nbsp;Ananya Sharma ,&nbsp;Arjun Chauhan ,&nbsp;Sumanpreet Kaur ,&nbsp;Jata Shankar","doi":"10.1016/j.crmicr.2024.100220","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100220","url":null,"abstract":"<div><p><em>Aspergillus</em> species encompass a variety of infections, ranging from invasive aspergillosis to allergic conditions, contingent upon the immune status of the host. In this spectrum, <em>Aspergillus terreus</em> stands out due to its emergence as a notable pathogen and its intrinsic resistance to amphotericin-B. The significance of <em>Aspergillus</em>-associated infections has witnessed a marked increase in the past few decades, particularly with the increasing number of immunocompromised individuals. The exploration of epidemiology, morphological transitions, immunopathology, and novel treatment approaches such as new antifungal drugs (PC945, olorofim) and combinational therapy using antifungal drugs and phytochemicals (Phytochemicals: quercetin, shikonin, artemisinin), also using immunotherapies to modulate immune response has resulted in better outcomes. Furthermore, in the context COVID-19 era and its aftermath, fungal infections have emerged as a substantial challenge for both immunocompromised and immunocompetent individuals. This is attributed to the use of immune-suppressing therapies during COVID-19 infections and the increase in transplant cases. Consequently, this review aims to provide an updated overview encompassing the epidemiology, germination events, immunopathology, and novel drug treatment strategies against <em>Aspergillus terreus-</em>associated infections.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100220"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000026/pdfft?md5=ede7f4424c3009102739c9f6f7deaf29&pid=1-s2.0-S2666517424000026-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The search for an antiviral lead molecule to combat the neglected emerging Oropouche virus","authors":"Rafaela dos Santos Peinado ,&nbsp;Marielena Vogel Saivish ,&nbsp;Gabriela de Lima Menezes ,&nbsp;Umberto Laino Fulco ,&nbsp;Roosevelt Alves da Silva ,&nbsp;Karolina Korostov ,&nbsp;Raphael Josef Eberle ,&nbsp;Paulo A. Melo ,&nbsp;Maurício Lacerda Nogueira ,&nbsp;Carolina Colombelli Pacca ,&nbsp;Raghuvir Krishnaswamy Arni ,&nbsp;Mônika Aparecida Coronado","doi":"10.1016/j.crmicr.2024.100238","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100238","url":null,"abstract":"<div><p>Oropouche virus (OROV) is a member of the <em>Peribunyaviridae</em> family and the causative agent of a dengue-like febrile illness transmitted by mosquitoes. Although mild symptoms generally occur, complications such as encephalitis and meningitis may develop. A lack of proper diagnosis, makes it a potential candidate for new epidemics and outbreaks like other known arboviruses such as Dengue, Yellow Fever and Zika virus. The study of natural molecules as potential antiviral compounds is a promising alternative for antiviral therapies. Wedelolactone (WDL) has been demonstrated to inhibit some viral proteins and virus replication, making it useful to target a wide range of viruses. In this study, we report the <em>in silico</em> effects of WDL on the OROV N-terminal polymerase and its potential inhibitory effects on several steps of viral infection in mammalian cells <em>in vitro</em>, which revealed that WDL indeed acts as a potential inhibitor molecule against OROV infection.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000208/pdfft?md5=6a565bd97ce9ada55b6058d5c19c4e96&pid=1-s2.0-S2666517424000208-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide","authors":"Imen Nouioui ,&nbsp;Judith Boldt ,&nbsp;Alina Zimmermann ,&nbsp;Roman Makitrynskyy ,&nbsp;Gabriele Pötter ,&nbsp;Marlen Jando ,&nbsp;Meike Döppner ,&nbsp;Sarah Kirstein ,&nbsp;Meina Neumann-Schaal ,&nbsp;Juan Pablo Gomez-Escribano ,&nbsp;Ulrich Nübel ,&nbsp;Yvonne Mast","doi":"10.1016/j.crmicr.2024.100290","DOIUrl":"10.1016/j.crmicr.2024.100290","url":null,"abstract":"<div><div>Actinomycetes are a prolific source of bioactive natural compounds many of which are used as antibiotics or other drugs. In this study we investigated the genomic and biochemical diversity of 32 actinobacterial strains that had been deposited at the DSMZ–German Collection of Microorganisms and Cell Cultures decades ago. Genome-based phylogeny and <em>in silico</em> DNA-DNA hybridization supported the assignment of these strains to 26 novel species and two novel subspecies and a reclassification of a <em>Streptomyces</em> species. These results were consistent with the biochemical, enzymatic, and chemotaxonomic features of the strains. Most of the strains showed antimicrobial activities against a range of Gram-positive and Gram-negative bacteria, and against yeast. Genomic analysis revealed the presence of numerous unique biosynthetic gene clusters (BGCs) encoding for potential novel antibiotic and anti-cancer compounds. Strains DSM 41636^T and DSM 61640^T produced the antibiotic compounds A33853 and SF2768, respectively. Overall, this reflects the significant pharmaceutical and biotechnological potential of the proposed novel type strains and underlines the role of prokaryotic systematics for drug discovery. In order to compensate for the gender gap in naming prokaryotic species, we propose the eponyms for all newly described species to honour female scientists.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100290"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the unique roles of extracellular vesicles for gut health modulation: Mechanisms, challenges, and perspectives","authors":"Qiming Wu ,&nbsp;Juntao Kan ,&nbsp;Caili Fu ,&nbsp;Xin Liu ,&nbsp;Zhengying Cui ,&nbsp;Sixu Wang ,&nbsp;Yi Le ,&nbsp;Zhanming Li ,&nbsp;Qin Liu ,&nbsp;Yuyu Zhang ,&nbsp;Jun Du","doi":"10.1016/j.crmicr.2024.100301","DOIUrl":"10.1016/j.crmicr.2024.100301","url":null,"abstract":"<div><div>Extracellular vesicles (EVs), which play significant regulatory roles in maintaining homeostasis and influencing immune responses, significantly impact gut microbiota composition and function, affecting overall gut health. Despite considerable progress, there are still knowledge gaps regarding the mechanisms by which EVs, including plant-derived EVs (PDEVs), animal-derived EVs (ADEVs), and microbiota-derived EVs (MDEVs), modulate gut health. This review delves into the roles and mechanisms of EVs from diverse sources in regulating gut health, focusing on their contributions to maintaining epithelial barrier integrity, facilitating tissue healing, eliciting immune responses, controlling pathogens, and shaping microbiota. We emphasize open challenges and future perspectives for harnessing EVs in the modulation of gut health to gain a deeper understanding of their roles and impact. Importantly, a comprehensive research framework is presented to steer future investigations into the roles and implications of EVs on gut health, facilitating a more profound comprehension of this emerging field.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100301"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}