Current Research in Microbial Sciences最新文献

{"title":"L-serine-O-sulfate alters cellular ultrastructure and mitigates the capacity of biofilm formation in Streptococcus mutans UA159 via interfering with glutamate racemase","authors":"Jianying Zhang ,&nbsp;He-Ling Wang ,&nbsp;Tianyu Ding ,&nbsp;Yingjie Sun ,&nbsp;Shaotai Wang ,&nbsp;Chengcheng Yin","doi":"10.1016/j.crmicr.2025.100427","DOIUrl":"10.1016/j.crmicr.2025.100427","url":null,"abstract":"<div><div>Dental caries, primarily caused by <em>Streptococcus mutans</em> (<em>S. mutans</em>), remains a significant global health challenge. Conventional treatments often disrupt commensal oral flora, necessitating targeted antimicrobial strategies. This study investigated L‑serine-O-sulfate (LSOS), a substrate analog of glutamate racemase (MurI), as a potential agent for interfering with <em>S. mutans</em> UA159 virulence. Computational docking predicted LSOS binding to MurI, while circular dichroism spectroscopy confirmed LSOS-induced structural perturbations in recombinant MurI. LSOS exhibited concentration-dependent bactericidal effects, with 5.0 mM completely suppressing growth and 2.5 mM significantly extending doubling time (11.37 hrs vs. 5.68 hrs in controls). Transmission electron microscopy revealed progressive ultrastructural damage, characterized by membrane blebs and cell wall disintegration. Biofilm formation was severely impaired, with 63 % reduction in biomass and significant disruption of extracellular matrix integrity. Microarray-based gene expression analysis identified 119 differentially expressed genes, predominantly downregulated (111/119), affecting translation machinery, metabolic pathways, and transmembrane transport. Biosafety evaluation in L929 fibroblasts showed reduced proliferation (67.59 % of control at 2.5 mM after 48 hrs) with both G1-phase reduction and S-phase cell cycle accumulation. <em>Caenorhabditis elegans</em> demonstrated uncompromised survival and early development at concentrations &lt;10 mM, with developmental toxicity emerging only at higher doses (≥20 mM). These findings establish LSOS as a promising anti-virulence agent targeting MurI in <em>S. mutans</em> UA159, with favorable biosafety profiles that warrant further investigation for dental caries prevention and treatment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100427"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaken not stirred - effect of different mixing modes during the cultivation of methanogenic pure cultures","authors":"Andja Mullaymeri,&nbsp;Maria Payr,&nbsp;Mathias Wunderer,&nbsp;Eva Maria Eva Maria,&nbsp;Andreas Otto Wagner","doi":"10.1016/j.crmicr.2025.100386","DOIUrl":"10.1016/j.crmicr.2025.100386","url":null,"abstract":"<div><div>Numerous cultivation techniques for aerobic microorganisms have been extensively investigated in the field of microbiology. Optimisation of these techniques is important for scientific and economic reasons. Methanogenic archaea, however, are obligate anaerobic microorganisms requiring different cultivation techniques than aerobic organisms due to the fundamental differences in physiology. Mixing of aerobic cultures is generally considered as very important as it provides organisms with essential oxygen; however, for anaerobic microorganisms lacking the ability to grow with oxygen, this point in cultivation was widely neglected. This work aimed at investigating the effect of different mixing modes on cultures of the methanogenic archaea <em>Methanomethylovorans thermophila, Methanosarcina acetivorans, Methanosarcina thermophila</em> and <em>Methanococcus vannielii</em> by cultivating them anaerobically in the modes standing/lying, shaken/unshaken and large/small serum flask in order to analyse their impact on the methane and biomass production. This study showed that a shaken incubation mode had a positive impact on methane production and resulted in its accelerated production, especially in hydrogenotrophic cultures; however, higher methane production did not necessarily lead to higher biomass production.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100386"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kodamaea ohmeri: An emergent yeast from a One Health perspective","authors":"Sthefany Emanuelle Silva ,&nbsp;Lorena Souza Silva ,&nbsp;Ludmila Gouveia Eufrasio ,&nbsp;Gabriela Silva Cruz ,&nbsp;Fabíola Lucini ,&nbsp;Hareton Teixeira Vechi ,&nbsp;Manoella do Monte Alves ,&nbsp;Luciana Rodrigues Ferreira Ribeiro ,&nbsp;Karine Lilian de Souza ,&nbsp;José Aparecido Moreira ,&nbsp;Janete Gouveia de Souza ,&nbsp;Florent Morio ,&nbsp;Gisela Lara da Costa ,&nbsp;Barbara de Oliveira Baptista ,&nbsp;Luiz Marcelo Ribeiro Tomé ,&nbsp;Sílvia Helena Sousa Pietra Pedroso ,&nbsp;Felipe Campos de Melo Iani ,&nbsp;Talita Émile Ribeiro Adelino ,&nbsp;Débora Castelo-Branco ,&nbsp;Luana Rossato ,&nbsp;Rafael Wesley Bastos","doi":"10.1016/j.crmicr.2025.100359","DOIUrl":"10.1016/j.crmicr.2025.100359","url":null,"abstract":"<div><div><em>Kodamaea ohmeri</em> is an emerging and opportunistic yeast associated with a high mortality rate in humans. As it is commonly found in the environment, it is possible that environmental conditions and agricultural practices contribute to the adaptation of this yeast and the selection of antifungal resistance. During a multicentric study in Brazil, conducted under a One Health perspective, 14 isolates of <em>K. ohmeri</em> were identified from different sources: three from blood cultures, three from animals (swine and poultry), and eight from animal environments (swine and poultry). Yeasts were isolated using CHROmagar® <em>Candida</em> medium and identified by MALDI-TOF MS and ITS rDNA barcoding. Minimum inhibitory concentration (MIC) was determined using the broth microdilution method for clinical (azoles, echinocandins, pyrimidine analogs, and polyenes), and environmental antifungals (tebuconazole, pyraclostrobin, carbendazim, and mancozeb), and hospital disinfectants (quaternary ammonium compounds). Of note, color variations of <em>K. ohmeri</em> were noted on CHROmagar® depending on the incubation time, which is likely to complicate its identification. Following polyphasic identification and taxonomic confirmation, all isolates demonstrated low MIC values for clinical antifungals, disinfectants, and tebuconazole. However, all isolates were able to grow in the presence of carbendazim, mancozeb, and pyraclostrobin. Together, these findings highlight the risks associated with the use of environmental azoles, such as tebuconazole, as they may impact non-target fungi of medical importance, but other fungicides do not present the same risk. This is the first study to demonstrate that <em>K. ohmeri</em>, an important emerging yeast in human medicine, can be isolated from various sources, including patients. Although the isolates exhibited low MIC values for clinical antifungals, it is crucial to monitor changes in sensitivity patterns over time in emerging microorganisms to prevent the development of multidrug resistance, which may originate in the environment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100359"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143535162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of isoprene by soil Actinomycetota from coffee-tea integrated plantations in a tropical evergreen forest","authors":"Toungporn Uttarotai ,&nbsp;Terry J. McGenity ,&nbsp;Sawannee Sutheeworapong ,&nbsp;Wuttichai Mhuantong ,&nbsp;Nuttapon Khongdee ,&nbsp;Sakunnee Bovonsombut ,&nbsp;Thararat Chitov","doi":"10.1016/j.crmicr.2025.100382","DOIUrl":"10.1016/j.crmicr.2025.100382","url":null,"abstract":"<div><div>Isoprene, a biogenic volatile compound emitted largely by plants, can form greenhouse gases when it reacts with atmospheric radicals. A significant amount of isoprene is absorbed into soil and can be degraded by soil microorganisms, but our understanding of the microbial biodegradation of isoprene in tropical ecosystems remains limited. This study investigated isoprene degradation by soil microbes indigenous to a tropical evergreen forest, focusing on those associated with coffee and tea plants grown as integrated crops and their genome characteristics in relation to their biodegradation capabilities. Following a 96-hour incubation with 7.2 × 10⁵ parts per billion by volume (ppbv) of isoprene, soil samples exhibited degradation levels ranging from 11.95 % to 36.54 %. From these soils, bacterial isolates belonging to the genera <em>Rhodococcus</em> and <em>Gordonia</em> (Actinomycetota) were recovered. These isolates demonstrated high isoprene biodegradation activity (50.3 %–69.1 % over seven days) and carried the <em>isoA</em> gene associated with isoprene metabolism. According to genome analysis, the organization of genes in the <em>iso</em> cluster was homologous, and the encoded amino acid sequences were highly similar to those of previously known isoprene-degrading members of the same genera. These findings emphasized the contribution of these widespread isoprene-degrading bacterial genera in the biodegradation of isoprene and the role of their isoprene monooxygenases in modulating atmospheric isoprene flux.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100382"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and identification of metabolites produced by Cytobacillus firmus in the interaction with Arabidopsis thaliana plants and their effect on Solanum lycopersicum","authors":"Itzel G. Arteaga-Ríos ,&nbsp;Karen Beatriz Méndez-Rodríguez ,&nbsp;Raul Ocampo-Pérez ,&nbsp;María de la Luz Guerrero-González ,&nbsp;Raúl Rodríguez-Guerra ,&nbsp;Pablo Delgado-Sánchez","doi":"10.1016/j.crmicr.2024.100312","DOIUrl":"10.1016/j.crmicr.2024.100312","url":null,"abstract":"<div><div>Currently, the use of bio-inputs is increasing due to the need to reduce the use of agrochemicals. However, one of the limitations is to preserve the viability of the living microorganisms, so it is important to find an alternative that allows us to obtain different metabolites to produce it. We evaluated three different interactions (contact, diffusible and volatile compounds) <em>in vitro</em> in <em>Arabidopsis thaliana</em> (At) seedlings with the strain <em>Cytobacillus firmus</em> M10 and its filtered secondary metabolites (M10F). The results showed that the seedlings inoculated by contact with the filtrate (AtM10F) presented increases in root length (30 %) and leaf area (33 %), as well as in the volatile interaction (At/M10F) with respect to the uninoculated treatment. For both interactions, the seedlings inoculated with the bacteria by contact (AtM10) and volatile (At/M10) obtained greater biomass (48 and 57 %). Subsequently, an evaluation at the end of the <em>A. thaliana</em> cycle showed that the treatments obtained by contact and distance when reinoculated with the bacteria and the filtrate (AtM10, At-M10 and AtM10F) obtained 50 % more seed yield than the control treatment, while AtM10F presented 72 %, while At/M10F presented the highest no. of siliques and seeds, which increased the yield by 65 %. In the <em>Solanum lycopersicum</em> (Sl) experiment, the filtrate (SlM10F) showed significant differences in seedling height, leaf length and width (23, 24 and 36 %, respectively). It also promoted an increase in fresh and dry weight, producing a greater root area and larger leaves compared to the control (Sl) and the bacteria (SlM10). We performed a qualitative characterization of the secondary metabolites present in the filtrate, where we found 2,4-DTBP, sylvopinol, isophthaladehyde, and eicosane of interest with possible growth-promoting effects on <em>A. thaliana</em> and tomato. We identified volatile compounds present in plant-microorganism and plant-filtrate interactions as possible precursors in the induction of plant growth, among which phenols, alcohols, aldehydes, alkanes, and alkenes stand out. Most of the analyzed compounds have not been found in the literature with reports of growth promoters, is important to mention that due to their characteristic functional groups they can derive and trigger the synthesis of new molecules with agronomic application.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100312"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in Acinetobacter baumannii","authors":"Sirui Zhang ,&nbsp;Jingdan Wang ,&nbsp;Rong Yu ,&nbsp;Haiping Liu ,&nbsp;Shuyan Liu ,&nbsp;Kai Luo ,&nbsp;Jin'e Lei ,&nbsp;Bei Han ,&nbsp;Yanjiong Chen ,&nbsp;Shaoshan Han ,&nbsp;E Yang ,&nbsp;Meng Xun ,&nbsp;Lei Han","doi":"10.1016/j.crmicr.2024.100332","DOIUrl":"10.1016/j.crmicr.2024.100332","url":null,"abstract":"<div><div>Although various mechanisms of carbapenem-resistance have been identified in the nosocomial pathogen <em>Acinetobacter baumannii</em>, the critical process of resistance evolution and the factors involved in are not well understood. Herein, we identified a universal stress protein Usp1413 which played an important role in adaptive resistance of <em>A. baumannii</em> to meropenem (MEM). Based on RNA-Seq and genome sequencing, Usp1413 was not only one of the most downregulated USPs, but also the bare one having mutation of tyrosine and glycine inserted at the site of 229-230 (YG229-230) under the stimulation of MEM. Deletion of Usp1413 resulted in increased MEM resistance. In addition, Usp1413 affected the bacterial abilities of biofilm formation and swarm motility, as well as helped <em>A. baumannii</em> response to various environmental stresses. These effects of Usp1413 were achieved by regulating its interaction proteins, within the functions of YigZ family protein, acetyltransferase, and SulP family inorganic anion transporter. The insertion mutation of YG229-230 influenced both the expression of interaction proteins and the phenotypes of bacteria. Finally, the promotor region of Usp1413 was convinced by point mutations. Overall, our findings identified the universal stress protein Usp1413 as a contributor involved in MEM adaptive resistance and responded to numerous environmental stresses. This study provides novel insights into the mechanism of universal stress proteins in participating antibiotic resistance, and affords a potential target for controlling drug resistance development in <em>A. baumannii</em>.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100332"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of carotenoid-producing Rhodotorula yeasts from amazonian substrates for sustainable biotechnology applications","authors":"Raissa Sayumy Kataki Fonseca ,&nbsp;Kevyn Melo Lotas ,&nbsp;Ana Claudia Alves Cortez ,&nbsp;Flávia da Silva Fernandes ,&nbsp;Érica Simplício de Souza ,&nbsp;Laurent Dufossé ,&nbsp;João Vicente Braga de Souza","doi":"10.1016/j.crmicr.2025.100373","DOIUrl":"10.1016/j.crmicr.2025.100373","url":null,"abstract":"<div><div>Carotenoids are natural pigments responsible for the yellow, orange, and red colors seen in various organisms. The aim of this study was to investigate the potential of yeast strains belonging to the genus <em>Rhodotorula</em> isolated from the Amazon Region in the production of carotenoids. Environmental samples from the Amazon Region were collected for yeast isolation. Isolates showing pigmented colonies underwent morphological and biochemical studies, as well as assessment of their potential for carotenoid production. The three best producers were identified through nucleotide sequencing of the ITS1–5.8s-ITS4 rDNA region. The top producer underwent univariate experiments to evaluate the influence of different C/N sources. Carotenoids produced were evaluated using CCD. Results showed the isolation of 13 pigmented yeasts with morphological and biochemical characteristics consistent with the genus <em>Rhodotorula.</em> Isolates RGM42 (601 μg/g), RTC42 (362 μg/g), and RTC45 (351 μg/g) stood out as the top carotenoid producers. These yeasts were identified as belonging to the species <em>Rhodotorula mucilaginosa</em>. Regarding C/N influence, <em>R. mucilaginosa</em> RGM42 produced the highest number of carotenoids using glucose and peptone as carbon and nitrogen sources, respectively. Isolate <em>R. mucilaginosa</em> RGM42 exhibited maximum growth with a peak at 72 h of bioprocess. Extracts from isolates <em>R. mucilaginosa</em> RGM42, <em>R. mucilaginosa</em> RTC42, <em>and R. mucilaginosa</em> RTC45 showed retention indices like β-carotene in thin-layer chromatography; however, <em>R. mucilaginosa</em> RGM42 presented an additional pigment in its chromatographic profile.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100373"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143643067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gracilimonas qinghaiensis sp. nov., a halophilic bacterium from a high-altitude saline lake exhibiting diverse metabolic potential and ecological adaptation","authors":"Haohao Wang ,&nbsp;Chi Zhang ,&nbsp;Yaqin Chen ,&nbsp;Yifan Guo ,&nbsp;Lijian Ding ,&nbsp;Shengfa Zhang ,&nbsp;Gaijun Du ,&nbsp;Weiyan Zhang ,&nbsp;Shan He","doi":"10.1016/j.crmicr.2025.100413","DOIUrl":"10.1016/j.crmicr.2025.100413","url":null,"abstract":"<div><div>Saline lakes are extreme habitats that host unique microbial communities with high biotechnological potential. In this study, a novel strain, designated Q87^T, was isolated from Gaxiukule Lake, a high-altitude magnesium sulfate-type saline lake in the Qaidam Basin, China. A polyphasic taxonomic approach, including morphological, physiological, chemotaxonomic, phylogenetic, and genomic analyses, was applied to characterize the isolate. Strain Q87^T is a Gram-stain-negative, non-motile, rod-shaped bacterium showing high tolerance to salinity (0–15.0 %, w/v; optimum 5.0 %) and alkalinity (pH 6.0–10.5; optimum pH 7.0), with a temperature range for growth of 10–40 °C (optimum 32 °C). Phylogenetic and genomic analyses confirmed its affiliation with the genus <em>Gracilimonas</em> and revealed it as a distinct species. The genome of strain Q87^T (3.3 Mb, <em>G</em> + <em>C</em> 41.5 %) encodes diverse functional genes associated with nitrogen and sulfur metabolism, stress adaptation, and biosynthesis of secondary metabolites, including terpenoids and polyketides. Comparative analyses with reference <em>Gracilimonas</em> strains demonstrated its unique genomic features and ecological adaptability. Structural modeling confirmed functional conservation of key enzymes involved in nitrogen detoxification and sulfide oxidation. Pangenome analysis highlighted the genetic diversity and open nature of the species of the genus <em>Gracilimonas</em>. Biogeographic assessments suggest a wide distribution of the genus in saline environments, especially in sediments. This study expands our understanding of the genus <em>Gracilimonas</em> taxonomy, physiology, and ecological potential, and underscores the importance of extremophilic bacteria as promising resources for environmental and industrial biotechnology.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100413"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144185491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Secretion of antifungal metabolites contributes to the antagonistic activity of Talaromyces oaxaquensis","authors":"Aneliz de Ita Zárate-Ortiz ,&nbsp;José Luis Villarruel-Ordaz ,&nbsp;Ana Claudia Sánchez-Espinosa ,&nbsp;Rommel A. Carballo-Castañeda ,&nbsp;Aldo Moreno-Ulloa ,&nbsp;Luis David Maldonado-Bonilla","doi":"10.1016/j.crmicr.2025.100402","DOIUrl":"10.1016/j.crmicr.2025.100402","url":null,"abstract":"<div><div>Controlling fusarium wilt is critical to guarantee the supply of bananas. In this study, three strains of the endophyte <em>Talaromyces oaxaquensis</em> obtained from pseudostems of <em>Musa</em> sp. AAB cv. Manzano were found to inhibit the <em>in vitro</em> growth of the banana pathogen <em>Fusarium oxysporum</em> f. sp. <em>cubense</em> M5. Constitutive production and secretion of antifungal factors by <em>T. oaxaquensis</em> is a feature of its inhibition effect, as there was no physical contact between the two fungi. An ethyl acetate extract prepared from supernatants of liquid cultures of the three <em>T. oaxaquensis</em> strains displayed inhibitory activity and hyphal swellings. Both the supernatants and mycelia were subjected to metabolic profiling. Penicillides and macrolide polyesters were detected in the supernatants. A molecular docking approach revealed the binding of the polyester 15G256α to chitin synthase 1 of <em>Fusarium oxysporum</em> f. sp. <em>cubense</em>. This is the first study to report the potential of the endophyte <em>Talaromyces</em> from <em>Musa</em> sp. to generate biological control products to protect plants against phytopathogens.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100402"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144105811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between dietary index for gut microbiota and sleep duration in US adults: a cross-sectional study","authors":"Jingjing Liu,&nbsp;Shaoqiang Huang","doi":"10.1016/j.crmicr.2025.100412","DOIUrl":"10.1016/j.crmicr.2025.100412","url":null,"abstract":"<div><h3>Background</h3><div>The microbiota-gut-brain axis underlies the pathogenesis of sleep disorders. The dietary index for gut microbiota (DI-GM) is an innovative diet quality metric related to gut microbiota diversity, with higher scores indicating a healthier gut microbiome. This study aimed to investigate its association with sleep duration.</div></div><div><h3>Methods</h3><div>This cross-sectional study analyzed the National Health and Nutrition Examination Survey (NHANES) data from 2005 to 2018, involving 25,439 participants aged ≥20 years. The DI-GM score was calculated utilizing dietary recall data, encompassing both beneficial (BGMS) and unfavorable (UGMS) to gut microbiota score. Sleep duration was assessed via the question: “How much sleep do you usually get at night on weekdays or workdays?” and categorized as short (&lt;7 h per night), optimal (7–8 h per night), and long (&gt;8 h per night). Multivariable weighted linear and logistic regression models were performed to determine the association between DI-GM and sleep duration. Secondary analyses included restricted cubic splines (RCS) and subgroup analyses.</div></div><div><h3>Results</h3><div>In the fully adjusted model, a higher DI-GM score was associated with longer sleep duration (β =0.03, 95 % confidence interval [CI]: 0.01–0.04, <em>P</em> &lt; 0.001), as was a higher BGMS (β =0.04, 95 % CI: 0.02–0.06, <em>P</em> &lt; 0.001). Using optimal sleep as the reference category, both higher DI-GM score and BGMS were significantly associated with lower odds of short sleep (DI-GM: odds ratio [OR] =0.96, 95 % CI: 0.93–0.99, <em>P</em> = 0.010; BGMS: OR =0.93, 95 % CI: 0.89–0.96, <em>P</em> &lt; 0.001). RCS revealed nonlinear associations between DI-GM and both outcomes, while BGMS exhibited linear patterns. Subgroup analyses confirmed consistent associations across demographic, lifestyle, and health strata, with significant effect modification by race/ethnicity (<em>P</em> = 0.015 for sleep duration; 0.016 for short sleep) and physical activity (<em>P</em> = 0.033 and 0.007, respectively).</div></div><div><h3>Conclusions</h3><div>Higher DI-GM scores were associated with longer sleep duration, underscoring the potential of gut microbiota-targeted diets for sleep health.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100412"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}