Current Research in Microbial Sciences最新文献

{"title":"Transcriptional control of two distinct lactococcal plasmid-encoded conjugation systems","authors":"Guillermo Ortiz Charneco ,&nbsp;Philip Kelleher ,&nbsp;Andrius Buivydas ,&nbsp;Paul P. de Waal ,&nbsp;Irma M.H. van Rijswijck ,&nbsp;Noël N.M.E. van Peij ,&nbsp;Jennifer Mahony ,&nbsp;Douwe Van Sinderen","doi":"10.1016/j.crmicr.2024.100224","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100224","url":null,"abstract":"<div><p>Lactococcal conjugative plasmids are poorly characterized compared to those harbored by numerous other Gram-positive bacteria, despite their significance in dairy fermentations and starter culture development. Furthermore, the transcriptional landscape of these lactococcal conjugation systems and their regulation have not been studied in any detail. Lactococcal plasmids pNP40 and pUC11B possess two genetically distinct and prevalent conjugation systems. Here, we describe the detailed transcriptional analysis of the pNP40 and pUC11B conjugation-associated gene clusters, revealing three and five promoters, respectively, for which the corresponding transcriptional start sites were identified. Regulation of several of these promoters, and therefore conjugation, is shown to involve the individual or concerted activities of the corresponding relaxase and transcriptional repressor(s) encoded by each conjugative plasmid. This work highlights how the conjugative potential of these systems may be unlocked, with significant implications for the starter culture and food fermentation industry.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100224"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000063/pdfft?md5=12e9b6f8499052f2e6a681321530633b&pid=1-s2.0-S2666517424000063-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139713782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota and bile acids: Metabolic interactions and impacts on diabetic kidney disease","authors":"Ping Liu ,&nbsp;Meiping Jin ,&nbsp;Ping Hu,&nbsp;Weiqian Sun,&nbsp;Yuyan Tang,&nbsp;Jiajun Wu,&nbsp;Dongliang Zhang,&nbsp;Licai Yang,&nbsp;Haidong He,&nbsp;Xudong Xu","doi":"10.1016/j.crmicr.2024.100315","DOIUrl":"10.1016/j.crmicr.2024.100315","url":null,"abstract":"<div><div>The intestinal microbiota comprises approximately 10¹³–10¹⁴ species of bacteria and plays a crucial role in host metabolism by facilitating various chemical reactions. Secondary bile acids (BAs) are key metabolites produced by gut microbiota.Initially synthesized by the liver, BA undergoes structural modifications through the activity of various intestinal microbiota enzymes, including eukaryotic, bacterial, and archaeal enzymes. These modified BA then activate specific receptors that regulate multiple metabolic pathways in the host, such as lipid and glucose metabolism, energy balance, inflammatory response, and cell proliferation and death. Recent attention has been given to intestinal flora disorders in diabetic kidney disease (DKD), where activation of BA receptors has shown promise in alleviating diabetic kidney damage by modulating renal lipid metabolism and mitochondrial production. Imbalances in the intestinal flora can influence the progression of DKD through the regulation of bile acid and its receptor pathways. This review aims to propose a mechanism involving the gut-BA-diabetes and nephropathy axes with the goal of optimizing new strategies for treating DKD.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100315"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possibilities and prospects of bioplastics production from agri-waste using bacterial communities: Finding a silver-lining in waste management","authors":"Mamun Mandal ,&nbsp;Anamika Roy ,&nbsp;Debasis Mitra ,&nbsp;Abhijit Sarkar","doi":"10.1016/j.crmicr.2024.100274","DOIUrl":"10.1016/j.crmicr.2024.100274","url":null,"abstract":"<div><p>To meet the need of the growing global population, the modern agriculture faces tremendous challenges to produce more food as well as fiber, timber, biofuels, etc.; hence generates more waste. This continuous growth of agricultural waste (agri-waste) and its management strategies have drawn the attention worldwide because of its severe environmental impacts including air, soil and water pollution. Similarly, growing concerns about the sustainable future have fuelled the development of biopolymers, substances occurring in and/or produced by living organisms, as substitute for different synthetic and harmful polymers, especially petroleum-based plastics. Now, the components of agri-waste offer encouraging opportunities for the production of bioplastics through mechanical and microbial procedures. Even the microbial, both bacterial and fungal, system results in lower energy consumption and better eco-friendly alternatives. The review mainly concentrates on cataloging and understanding the bacterial 'input' in developing bioplastics from diverse agri-waste. Especially, the bacteria like <em>Cupriavidus necator, Chromatium vinosum</em>, and <em>Pseudomonas aeruginosa</em> produce short- and medium-chain length poly(3-hydroxyalkanote) (P3HB) polymers using starch (from corn and potato waste), and cellulose (from sugarcane bagasse, corn husks waste). Similarly, <em>C. necator</em>, and transformant <em>Wautersia eutropha</em> produce P3HB polymer using lipid-based components (such as palm oil waste). Important to note that, the synthesis of these polymers are interconnected with the bacterial general metabolic activities, for example Krebs cycle, glycolysis cycle, <em>β</em>-oxidation, calvin cycle, <em>de novo</em> fatty acid syntheses, etc. Altogether, the agri-waste is reasonably low-cost feed for the production of bioplastics using bacterial communities; and the whole process certainly provide an opportunity towards sustainable waste management strategy.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100274"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000567/pdfft?md5=9addcc7be36a71cc5fdecb58fe6a7c99&pid=1-s2.0-S2666517424000567-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the landscape of biofertilizers containing plant growth-promoting rhizobacteria in Iran: Progress and research prospects","authors":"Houshang Khosravi ,&nbsp;Bahman Khoshru ,&nbsp;Alireza Fallah Nosratabad ,&nbsp;Debasis Mitra","doi":"10.1016/j.crmicr.2024.100268","DOIUrl":"10.1016/j.crmicr.2024.100268","url":null,"abstract":"<div><p>The use of chemical fertilizers in agriculture, while effective for plant nutrition, poses environmental pollution risks due to their overuse and imbalance. Sustainable agricultural solutions are required to reduce reliance on chemical fertilizers. Biofertilizers, which harness the potential of beneficial soil microorganisms, are a promising alternative. Plant growth-promoting rhizobacteria (PGPR) are beneficial soil bacteria for the preparing of biofertilizers, as they enhance plant growth through various mechanisms, such as nitrogen fixation, hormone production, and nutrient solubilization. Although significant results have been achieved in laboratory and greenhouse experiments, field studies are relatively limited. In the last three decades, research has been conducted on PGPR in Iran but The Soil and Water Research Institute (SWRI) has been a focal point for concentrated and continuous research on PGPR biofertilizers, resulting in the development of various biofertilizers containing <em>Azotobacter, Pseudomonas</em>, and <em>Bacillus</em> strains. In any case, biofertilizers still do not have a significant place in Iran's agriculture compared to chemical fertilizers. Challenges to the research, development, and adoption of PGPR biofertilizers in Iran include reliance on chemical fertilizers, low soil organic matter, and limited coordination among research, production, and extension efforts. However, there are opportunities for growth, including skilled professionals, technical knowledge, and existing infrastructure in the private sector, as well as an increasing demand for organic and sustainable products. To further develop PGPR biofertilizers in Iran, it is recommended to increase soil organic matter, conduct molecular research to enhance PGPR efficiency, establish research extension farms, and promote the benefits of biofertilizer use. These measures could contribute to broader adoption of biofertilizers and sustainable agricultural practices in Iran.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100268"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000506/pdfft?md5=8bc568e5a41bf45bb42753f78030f4a8&pid=1-s2.0-S2666517424000506-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Draft genome sequence of Agrobacterium pusense strain CMT1: A promising growth-promoting bacterium isolated from nodules of soybean (Glycine max L. Merrill) crops for the One Health approach in Paraguay","authors":"Antonio Samudio Oggero ,&nbsp;Magalí Valdez Borda ,&nbsp;Carmen María Félix Pablos ,&nbsp;Gladis Leguizamón ,&nbsp;Fernando Mathías Morínigo ,&nbsp;Víctor González Caballero ,&nbsp;Ariel Farías ,&nbsp;Héctor Nakayama ,&nbsp;Sergio de los Santos Villalobos","doi":"10.1016/j.crmicr.2024.100259","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100259","url":null,"abstract":"<div><p>Strain CMT1 was isolated from nodules of non-inoculated Roundup Ready (RR) soybean plants (<em>Glycine max</em> L. Merrill), which were collected in fields in Itauguá, Paraguay. The genome of this strain had 338,984,909 bp; 59.2 % G + C content; 377648 bp N50; 5 L50; 55 contigs; 51 RNAs and 5,272 predicted coding DNA sequences (CDS) distributed in 327 subsystems. Based on overall genome-relatedness indices (OGRIs), this strain was taxonomically affiliated with <em>Agrobacterium pusense</em>. Based on genome mining, strain CMT1 is a promising plant growth-promoting bacterium that could be validated in agricultural fields for increasing soybean yield and quality, diminishing the economic, environmental, and health costs of non-sustainable food production.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100259"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000415/pdfft?md5=6a9526b8181f50e59084e65f8fc9f4b6&pid=1-s2.0-S2666517424000415-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141583054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-bleaching of ankara pulp with xylanase-producing bacterial consortium for sustainable handmade paper production","authors":"Meenakshi Rajput ,&nbsp;Disha Pamecha ,&nbsp;Preeti Kumari ,&nbsp;Payal Chaturvedi ,&nbsp;Charu Sharma ,&nbsp;Rahul Mishra ,&nbsp;Sunita Chauhan","doi":"10.1016/j.crmicr.2024.100262","DOIUrl":"10.1016/j.crmicr.2024.100262","url":null,"abstract":"<div><p>The paper industry faces two critical challenges: the scarcity of raw materials and the environmental impact of chemical waste pollution. Addressing the first challenge involves harnessing alternative, sustainable raw materials, while the second challenge can be mitigated through the adoption of bio-bleaching processes, which significantly reduce chemical consumption while enhancing paper brightness and quality. This study proposes a solution to both challenges by using non-woody Calotropis procera (Ankara) and a xylanase-producing microbial consortium for sustainable handmade paper production, a combination not extensively explored in prior research<strong>.</strong> To evaluate this approach, the process was divided into three stages. In stage I, Ankara fibre was pulped through open hot digestion. In stage II, the pulp was subjected to bio-bleaching in two experimental setups: Set I (without sucrose) and Set II (with sucrose) for 5 days. In stage III, chemical bleaching was used to improve the final brightness of the treated pulps. A novel comparison was made between the bio-bleaching efficiency of an individual isolate g5 (BI) and a bacterial consortium (BC). This research highlighted that bio-bleaching with the consortium effectively removed lignin (140±60 mg/l) and colour (1830±50 PCU), especially in the presence of sucrose, compared to using a single xylanase isolate. Pulp residue/filtrate collected at each stage was estimated based on parameters such as colour and lignin content. After stage III (chemical bleaching), the release of colour and lignin in pulp filtrate was higher in BI compared to BC, indicating the consortium's effectiveness during bio-bleaching, which leaves fewer degradable lignin structures for the chemical bleaching stage. Papers crafted from consortium-treated pulp also exhibited higher brightness than those treated with the isolate. This study reveals the synergistic effect of microbial consortia, leading to more efficient lignin degradation and enhanced bio-bleaching capabilities, supporting the development of greener industrial processes. Ultimately, this study demonstrates a unique and eco-friendly approach to papermaking, combining C. procera and enzymatic bio-bleaching to reduce dependency on hazardous chemicals and support sustainable industry practices.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100262"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000440/pdfft?md5=4fba487da2a96ee8a64f608418969f5e&pid=1-s2.0-S2666517424000440-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141693733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADS-J21 is a novel HIV-1 entry inhibitor targeting gp41","authors":"Ruiying Liang ,&nbsp;Dou Dou ,&nbsp;Chunying Wang ,&nbsp;Shanshan Huo ,&nbsp;Yang Wu ,&nbsp;Juan Wang ,&nbsp;Zhengsen Yu ,&nbsp;Shuomin Zhang ,&nbsp;Jingjing Xu ,&nbsp;Yue Liu ,&nbsp;Peng Liu ,&nbsp;Shibo Jiang ,&nbsp;Fei Yu","doi":"10.1016/j.crmicr.2024.100260","DOIUrl":"10.1016/j.crmicr.2024.100260","url":null,"abstract":"<div><p>HIV-1 envelope glycoprotein gp41 mediates fusion between HIV-1 and host cell membranes, making inhibitors of gp41 attractive anti-HIV drugs. We previously reported an efficient HIV-1 fusion inhibitor, ADS-J1, with a Y-shaped structure. Here, we discovered a new compound, ADS-J21, with a Y-shaped structure similar to that of ADS-J1 but with a lower molecular weight. Moreover, ADS-J21 exhibited effective anti-HIV-1 activity against divergent HIV-1 strains <em>in vitro</em>, including several HIV-1 laboratory-adapted strains and primary isolates with different subtypes (clades A to F) and tropisms (X4 or R5). Mechanistic studies have demonstrated that ADS-J21 blocks the formation of the gp41 six-helix bundle (6-HB) by targeting conserved amino acids Lys35 and Trp32. These findings suggest that ADS-J21 can be used as a new lead compound for further optimization in the development of a small-molecule fusion inhibitor.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100260"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000427/pdfft?md5=66eb91c4adefa23816fce7efab38647e&pid=1-s2.0-S2666517424000427-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141697766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crop residue heterogeneity: Decomposition by potential indigenous ligno-cellulolytic microbes and enzymatic profiling","authors":"Sandeep Sharma ,&nbsp;Kailash Chand Kumawat ,&nbsp;Paawan Kaur ,&nbsp;Sukhjinder Kaur ,&nbsp;Nihar Gupta","doi":"10.1016/j.crmicr.2024.100227","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100227","url":null,"abstract":"<div><p>The continuous depletion of fossil resources, energy-crisis and environmental pollution has gained popularity for careful selection of suitable microbial consortium to efficiently decompose crop residue and facilitate nutrient cycling. While crop residue is commonly incorporated into soil, the impact of the heterogeneity of residue on decomposition and biological mechanisms involved in extracellular carbon (C) cycle related enzyme activities remain not fully understood. To address this problem, an incubation study was conducted on chemical heterogeneity of straw and root residue with indigenous ligno-cellulolytic microbial consortium on extracellular enzymes as their activity is crucial for making <em>in-situ</em> residue management decisions under field condition. The activity of extracellular enzymes in different substrates showed differential variation with the type of enzyme and ranged from 16.9 to 77.6 µg mL⁻¹, 135.7 to 410.8 µg mL⁻¹, 66.9 to 177.1 µg mL⁻¹ and 42.1 to 160.9 µg mL⁻¹ for cellulase, xylanase, laccase and lignin peroxidase, respectively. Extracellular enzyme activities were sensitive to heterogeneity of biochemical constituent's present in straw and root residues and enhanced the decomposition processes with indigenous ligno-cellulolytic microbial consortium (<em>Bacillus altitudinis, Streptomyces flavomacrosporus</em> and <em>Aspergillus terreus</em>). Correlation matrix elucidated <em>A. terreus</em> and <em>B. altitudinis</em> as potential indigenous ligno-cellulolytic microbial inoculant influencing soil enzymatic activity (<em>p</em> &lt; 0.001). This research work demonstrates a substantial impact of chemically diverse crop residues on the decomposition of both straw and root. It also highlights the pivotal role played by key indigenous decomposers and interactions between different microorganisms in governing the decomposition of straw and root primarily through release of extracellular enzyme. Consequently, it is novel bio-emerging strategy suggested that incorporation of the crop residues under field conditions should be carried out in conjunction with the potential indigenous ligno-cellulolytic microbial consortium for efficient decomposition in the short period of time under sustainable agriculture system.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100227"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000099/pdfft?md5=0ec104dde22613a002ce7415186cc1c4&pid=1-s2.0-S2666517424000099-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140000301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of MDR/XDR-TB on the global tuberculosis epidemic: Challenges and opportunities","authors":"Kai Ling Chin ,&nbsp;Luis Anibarro ,&nbsp;Zi Yuan Chang ,&nbsp;Praneetha Palasuberniam ,&nbsp;Zainal Arifin Mustapha ,&nbsp;Maria E. Sarmiento ,&nbsp;Armando Acosta","doi":"10.1016/j.crmicr.2024.100295","DOIUrl":"10.1016/j.crmicr.2024.100295","url":null,"abstract":"<div><div>Tuberculosis (TB) is the world's second-deadliest infectious disease. Despite the availability of drugs to cure TB, control of TB is hampered by the emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB). The presence of MDR/XDR-TB is alarming due to the low detection rate, high treatment failure, and high mortality. The increasing cases of MDR/XDR-TB are mainly due to the limitations in the diagnostic tests to detect the drug susceptibility of the pathogen, which contribute to the spread of the disease through close contacts. Moreover, inconsistent drug therapy or unsuitable drug regimens could also lead to the subsequent development of drug resistance. The close contacts of an index MDR/XDR-TB patient are at increased risk of developing MDR/XDR-TB. Also, the BCG vaccine may exhibit varying protective effects due to BCG strain diversification, host immune status, exposure to environmental non-tuberculous mycobacteria (NTM), and differences in <em>Mycobacterium tuberculosis</em> (Mtb) subspecies infection, as in the case of sub-optimal protection in the case of Beijing family genotypes of Mtb. This review provides an overview of the current state of drug-resistant tuberculosis (DR-TB) within the context of the global TB pandemic, with a focus on diagnosis, treatment, and the potential impact of BCG vaccination. It highlights the limitations of current approaches and aims to identify opportunities for improving TB control strategies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100295"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transdisciplinary approaches for the study of cyanobacteria and cyanotoxins","authors":"Roel Alejandro Chávez-Luzanía ,&nbsp;María Edith Ortega-Urquieta ,&nbsp;Jaquelyn Aguilera-Ibarra ,&nbsp;Pamela Helué Morales-Sandoval ,&nbsp;José Antonio Hernández-Coss ,&nbsp;Luis Alberto González-Vázquez ,&nbsp;Vielka Berenice Jara-Morales ,&nbsp;Sergio Hiram Arredondo-Márquez ,&nbsp;Marie Jennifer Olea-Félix ,&nbsp;Sergio de los Santos-Villalobos","doi":"10.1016/j.crmicr.2024.100289","DOIUrl":"10.1016/j.crmicr.2024.100289","url":null,"abstract":"<div><div>Cyanobacteria, ancient aerobic and photoautotrophic prokaryotes, thrive in diverse ecosystems due to their extensive morphological and physiological adaptations. They play crucial roles in aquatic ecosystems as primary producers and resource providers but also pose significant ecological and health risks through blooms that produce harmful toxins, called cyanotoxins. The taxonomic affiliation of cyanobacteria has evolved from morphology-based methods to genomic analysis, which offers detailed structural and physiological insights that are essential for accurate taxonomic affiliation and monitoring. However, challenges posed by uncultured species have been extrapolated to the detection and quantification of cyanotoxins. Current advances in molecular biology and informatics improve the precision of monitoring and allow the analysis of groups of genes related to toxin production, providing crucial information for environmental biosafety and public health. Unfortunately, public genomic databases heavily underrepresent cyanobacteria, which limits the understanding of their diversity and metabolic capabilities. Despite the increasing availability of cyanobacterial genome sequences, research is still largely focused on a few model strains, narrowing the scope of genetic and metabolic studies. The challenges posed by cyanobacterial blooms and cyanotoxins necessitate improved molecular, cultivation, and polyphasic techniques for comprehensive classification and quantification, highlighting the need for advanced genomic approaches to better understand and manage cyanobacteria and toxins. This review explores the application of transdisciplinary approaches for the study of cyanobacteria and cyanotoxins focused on diversity analysis, population quantification, and cyanotoxin monitoring, emphasizing their genomic resources and their potential in the genomic mining of toxin-related genes.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100289"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}