Current Research in Microbial Sciences最新文献

{"title":"Gut microbiome derived short chain fatty acids: Promising strategies in necrotising enterocolitis","authors":"María P Cifuentes,&nbsp;Jonathan A Chapman,&nbsp;Christopher J Stewart","doi":"10.1016/j.crmicr.2024.100219","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100219","url":null,"abstract":"<div><p>Necrotising enterocolitis (NEC) is a devastating condition that poses a significant risk of morbidity and mortality, particularly among preterm babies. Extensive research efforts have been directed at identifying optimal treatment and diagnostic strategies but results from such studies remain unclear and controversial. Among the most promising candidates are prebiotics, probiotics and their metabolites, including short chain fatty acids (SCFAs). Such metabolites have been widely explored as possible biomarkers of gut health for different clinical conditions, with overall positive effects on the host observed. This review aims to describe the role of gut microbiome derived SCFAs in necrotising enterocolitis. Until now, information has been conflicting, with the primary focus on the main three SCFAs (acetic acid, propionic acid, and butyric acid). While numerous studies have indicated the relationship between SCFAs and NEC, the current evidence is insufficient to draw definitive conclusions about the use of these metabolites as NEC biomarkers or their potential in treatment strategies. Ongoing research in this area will help enhance both our understanding of SCFAs as valuable indicators of NEC and their practical application in clinical settings.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100219"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000014/pdfft?md5=02d4ab5c6144264da0b19485693ef433&pid=1-s2.0-S2666517424000014-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139494070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging frontiers in microbial-mediated utilization of crop residues for economically valuable biomaterials","authors":"Debasis Mitra ,&nbsp;Periyasamy Panneerselvam ,&nbsp;Pradeep K.Das Mohapatra ,&nbsp;Marika Pellegrini ,&nbsp;G. Selvakumar","doi":"10.1016/j.crmicr.2024.100225","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100225","url":null,"abstract":"<div><p>Agricultural crop residues include leftover and unmarketable materials, such as crop stover, weeds, leaf litter, sawdust, forest litter, and livestock manure originating from crop cultivation, and post-harvest activities. Such residues are a storehouse of plant nutrients and several other resources and therefore need to be managed in an environment- friendly manner with minimum loss of plant nutrients and other resources that can be recovered. Microbial starter consortia are a key component in the rapid recycling of farm residue wastes and the production of other valuable products, such as biogas, bioethanol/biofuel, enzymes, molecules, and metabolites. Recent advances in microbial biotechnology can also facilitate the conversion of farm residues into economically valuable materials, <em>i.e.</em> soil additives, adsorbents, energy, and enzymes, thereby contributing to a circular economy. This special issue attempts to compile the latest advancements in the field of agricultural crop residue management for enhanced nutrient recycling and resource recovery by the use of compost starters and inoculant formulations.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100225"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000075/pdfft?md5=f5914c29ace76ce2a6b0eeb026644d2c&pid=1-s2.0-S2666517424000075-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139737633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic investigation unveils high-risk ESBL producing Enterobacteriaceae within a rural environmental water body","authors":"P S Seethalakshmi ,&nbsp;Vishnu Prasad Nair RU ,&nbsp;Anushara Prabhakaran ,&nbsp;Ragothaman Prathiviraj ,&nbsp;Rajesh Pamanji ,&nbsp;George Seghal Kiran ,&nbsp;Joseph Selvin","doi":"10.1016/j.crmicr.2023.100216","DOIUrl":"https://doi.org/10.1016/j.crmicr.2023.100216","url":null,"abstract":"<div><p>Antimicrobial resistance is regarded as a global threat to public health, animals, and the environment, emerging in response to extensive utilization of antimicrobials. The determinants of antimicrobial resistance are transported to susceptible bacterial populations through genetic recombination or through gene transfer, mediated by bacteriophages, plasmids, transposons, and insertion sequences. To determine the penetration of antimicrobial resistance into the bacterial population of the Thiruvandarkoil Lake, a water body located in the rural settings of Puducherry, India, culture-based microbiological and genomic approaches were used. Resistant bacterial isolates obtained from microbiological screening were subjected to whole genome sequencing and the genetic determinants of antimicrobial resistance were identified using <em>in silico</em> genomic tools. Cephalosporin-resistant isolates were found to produce extended spectrum beta lactamases, encoded by bla_VEB-6 (in <em>Proteus mirabilis</em> PS01), bla_SHV-12 and <em>ompK</em>36 mutation (in <em>Klebsiella quasipneumoniae</em> PS02) and bla_SHV-12, bla_ACT-16, bla_CTX₋_M and bla_NDM-1 in (<em>Enterobacter hormaechei</em> PS03). Genes encoding heavy metal resistance, virulence and resistance to detergents were also detected in these resistant isolates. Among ESBL-producing organisms, one <em>mcr-9</em>-positive <em>Enterobacter hormaechei</em> was also identified in this study. To our knowledge, this is the first report of <em>mcr-9</em> carrying bacterium in the environment in India. This study seeks the immediate attention of policy makers, researchers, government officials and environmental activists in India, to develop surveillance programs to monitor the dissemination of antimicrobial resistance in the environment.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100216"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517423000378/pdfft?md5=3550cf0311d007d7400ebc7284dbd07c&pid=1-s2.0-S2666517423000378-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139109226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transdisciplinary approaches for the study of cyanobacteria and cyanotoxins","authors":"Roel Alejandro Chávez-Luzanía ,&nbsp;María Edith Ortega-Urquieta ,&nbsp;Jaquelyn Aguilera-Ibarra ,&nbsp;Pamela Helué Morales-Sandoval ,&nbsp;José Antonio Hernández-Coss ,&nbsp;Luis Alberto González-Vázquez ,&nbsp;Vielka Berenice Jara-Morales ,&nbsp;Sergio Hiram Arredondo-Márquez ,&nbsp;Marie Jennifer Olea-Félix ,&nbsp;Sergio de los Santos-Villalobos","doi":"10.1016/j.crmicr.2024.100289","DOIUrl":"10.1016/j.crmicr.2024.100289","url":null,"abstract":"<div><div>Cyanobacteria, ancient aerobic and photoautotrophic prokaryotes, thrive in diverse ecosystems due to their extensive morphological and physiological adaptations. They play crucial roles in aquatic ecosystems as primary producers and resource providers but also pose significant ecological and health risks through blooms that produce harmful toxins, called cyanotoxins. The taxonomic affiliation of cyanobacteria has evolved from morphology-based methods to genomic analysis, which offers detailed structural and physiological insights that are essential for accurate taxonomic affiliation and monitoring. However, challenges posed by uncultured species have been extrapolated to the detection and quantification of cyanotoxins. Current advances in molecular biology and informatics improve the precision of monitoring and allow the analysis of groups of genes related to toxin production, providing crucial information for environmental biosafety and public health. Unfortunately, public genomic databases heavily underrepresent cyanobacteria, which limits the understanding of their diversity and metabolic capabilities. Despite the increasing availability of cyanobacterial genome sequences, research is still largely focused on a few model strains, narrowing the scope of genetic and metabolic studies. The challenges posed by cyanobacterial blooms and cyanotoxins necessitate improved molecular, cultivation, and polyphasic techniques for comprehensive classification and quantification, highlighting the need for advanced genomic approaches to better understand and manage cyanobacteria and toxins. This review explores the application of transdisciplinary approaches for the study of cyanobacteria and cyanotoxins focused on diversity analysis, population quantification, and cyanotoxin monitoring, emphasizing their genomic resources and their potential in the genomic mining of toxin-related genes.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100289"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of MDR/XDR-TB on the global tuberculosis epidemic: Challenges and opportunities","authors":"Kai Ling Chin ,&nbsp;Luis Anibarro ,&nbsp;Zi Yuan Chang ,&nbsp;Praneetha Palasuberniam ,&nbsp;Zainal Arifin Mustapha ,&nbsp;Maria E. Sarmiento ,&nbsp;Armando Acosta","doi":"10.1016/j.crmicr.2024.100295","DOIUrl":"10.1016/j.crmicr.2024.100295","url":null,"abstract":"<div><div>Tuberculosis (TB) is the world's second-deadliest infectious disease. Despite the availability of drugs to cure TB, control of TB is hampered by the emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB). The presence of MDR/XDR-TB is alarming due to the low detection rate, high treatment failure, and high mortality. The increasing cases of MDR/XDR-TB are mainly due to the limitations in the diagnostic tests to detect the drug susceptibility of the pathogen, which contribute to the spread of the disease through close contacts. Moreover, inconsistent drug therapy or unsuitable drug regimens could also lead to the subsequent development of drug resistance. The close contacts of an index MDR/XDR-TB patient are at increased risk of developing MDR/XDR-TB. Also, the BCG vaccine may exhibit varying protective effects due to BCG strain diversification, host immune status, exposure to environmental non-tuberculous mycobacteria (NTM), and differences in <em>Mycobacterium tuberculosis</em> (Mtb) subspecies infection, as in the case of sub-optimal protection in the case of Beijing family genotypes of Mtb. This review provides an overview of the current state of drug-resistant tuberculosis (DR-TB) within the context of the global TB pandemic, with a focus on diagnosis, treatment, and the potential impact of BCG vaccination. It highlights the limitations of current approaches and aims to identify opportunities for improving TB control strategies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100295"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiological dimensions and functions in constructed wetlands: A review","authors":"Anju Rani ,&nbsp;Mansi Chauhan ,&nbsp;Pradeep Kumar Sharma ,&nbsp;Mansi Kumari ,&nbsp;Debasis Mitra ,&nbsp;Samiksha Joshi","doi":"10.1016/j.crmicr.2024.100311","DOIUrl":"10.1016/j.crmicr.2024.100311","url":null,"abstract":"<div><div>Constructed wetlands (CWs) have emerged as robust alternatives to traditional wastewater treatment technologies. The efficacy of CWs centers on the intricate interplay of factors, including plant species, substrate attributes, soil conditions, microbial consortia, and operational variables. Microorganisms, the cornerstone of CWs, have a significant influence on mineralization and pollutant mitigation processes. Microbial assemblies within CWs exhibit dynamic responses by adapting novel mechanisms. Here, Proteobacteria possess the ability to efficiently fix nitrogen through innovative pathways, including denitrifying anaerobic methane oxidation (DAMO) and sulfur autotrophic denitrification (SAD). Furthermore, Actinobacteria, Firmicutes, and Gemmatimonadetes have been thoroughly investigated to emphasize their significance in phosphate metabolism. These microorganisms may adapt to various metabolic pathways due to seasonal changes, plant diversity, wastewater composition, and system configuration. On the other hand, microorganisms have been proven to be effective in removing heavy metals in constructed wetlands. Various techniques such as biosorption, transformation, and biomineralization are widely used for this purpose. Therefore, it is essential to conduct a comprehensive investigation of the structure and diversity of microbial communities within constructed wetlands to gain a complete understanding of the system's performance and optimization strategies. This review provides a detailed analysis of microbial studies conducted in constructed wetlands and aimed to understand the mechanisms involved in pollutant removal and the influence of various factors on microbial community prevalence. The outcomes of this review not only deepen our comprehension of CWs and ecosystems, but also provide a basis for knowledgeable choices in their planning and execution, ultimately leading to the responsible stewardship of water resources.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100311"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gamma radiation-induced enhancement of biocontrol agents for plant disease management","authors":"Mahsa Rostami,&nbsp;Abozar Ghorbani,&nbsp;Samira Shahbazi","doi":"10.1016/j.crmicr.2024.100308","DOIUrl":"10.1016/j.crmicr.2024.100308","url":null,"abstract":"<div><div>Gamma radiation-induced mutations in microorganisms can enhance their properties for the biological control of plant diseases. Mutant strains of <em>Bacillus subtilis</em> were found to have improved antifungal properties against <em>Aspergillus flavus</em> and increased production of biosurfactants and biofilms. Furthermore, combining gamma radiation with antagonists was more effective in controlling <em>Penicillium expansum</em> postharvest than either treatment alone. A major focus of this research was on <em>Trichoderma</em> species, which have shown an enhanced ability to control plant diseases through increased production of antifungal metabolites such as hydrolytic enzymes, antibiotics, and total phenols. The mechanism by which gamma radiation alters the genotype of microorganisms is the destruction of double-stranded and single-stranded DNA, resulting in changes in the genome or nucleic acid molecule, altering the antagonistic properties of microorganisms. Sensitivity to radiation is determined by the size of an organism's chromosomes, and the effect on microorganisms is primarily based on DNA or RNA disruption. Molecular analysis of gamma radiation mutants has been used to understand changes in genome composition, including downregulated genes related to secondary metabolism, cytochrome P450 s, carbohydrate-active enzymes, peptidases, and hydrophobins. Gamma radiation thus offers a promising method to induce beneficial genetic changes in microorganisms, enhancing their efficacy in the biological control of plant diseases.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100308"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxoplasma–host endoplasmic reticulum interaction: How T. gondii activates unfolded protein response and modulates immune response","authors":"Obed Cudjoe ,&nbsp;Roger Afful ,&nbsp;Tonny Abraham Hagan","doi":"10.1016/j.crmicr.2024.100223","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100223","url":null,"abstract":"<div><p><em>Toxoplasma gondii</em> is a neurotropic single–celled zoonotic parasite that can infect human beings and animals. Infection with <em>T. gondii</em> is usually asymptomatic in immune–competent individual, however, it can cause symptomatic and life–threatening conditions in immunocompromised individuals and in developing foetuses. Although the mechanisms that allow <em>T. gondii</em> to persist in host cells are poorly understood, studies in animal models have greatly improved our understanding of <em>Toxoplasma</em>–host cell interaction and how this interaction modulates parasite proliferation and development, host immune response and virulence of the parasite. <em>T. gondii</em> is capable of recruiting the host endoplasmic reticulum (ER), suggesting it may influence the host ER function. Herein, we provide an overview of <em>T. gondii</em> infection and the role of host ER during stressed conditions. Furthermore, we highlight studies that explore <em>T. gondii</em>'s interaction with the host ER. We delve into how this interaction activates the unfolded protein response (UPR) and ER stress-mediated apoptosis. Additionally, we examine how <em>T. gondii</em> exploits these pathways to its advantage.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100223"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000051/pdfft?md5=6786a7352f986174c4da1a5309d3b207&pid=1-s2.0-S2666517424000051-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139675986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Campus source to sink wastewater surveillance of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)","authors":"M. Folkes ,&nbsp;V.M. Castro-Gutierrez ,&nbsp;L. Lundy ,&nbsp;Y. Bajón-Fernández ,&nbsp;A. Soares ,&nbsp;P. Jeffrey ,&nbsp;F. Hassard","doi":"10.1016/j.crmicr.2024.100240","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100240","url":null,"abstract":"<div><p>Wastewater-based surveillance (WBS) offers an aggregate, and cost-effective approach for tracking infectious disease outbreak prevalence within communities, that provides data on community health complementary to individual clinical testing. This study reports on a 16-month WBS initiative on a university campus in England, UK, assessing the presence of SARS-CoV-2 in sewers from large buildings, downstream sewer locations, raw wastewater, partially treated and treated effluents. Key findings include the detection of the Alpha (B.1.1.7) variant in wastewater, with 70 % of confirmed campus cases correlating with positive wastewater samples. Notably, ammonium nitrogen (NH₄-N) levels showed a positive correlation (ρ = 0.543, <em>p</em> &lt; 0.01) with virus levels at the large building scale, a relationship not observed at the sewer or wastewater treatment works (WWTW) levels due to dilution. The WWTW was compliant to wastewater standards, but the secondary treatment processes were not efficient for virus removal as SARS-CoV-2 was consistently detected in treated discharges. Tools developed through WBS can also be used to enhance traditional environmental monitoring of aquatic systems. This study provides a detailed source-to-sink evaluation, emphasizing the critical need for the widespread application and improvement of WBS. It showcases WBS utility and reinforces the ongoing challenges posed by viruses to receiving water quality.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100240"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000221/pdfft?md5=8dfcff80d95928a8845ee203be089fce&pid=1-s2.0-S2666517424000221-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140901369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro antifungal activity of MMV Pathogen Box® compounds alone or in combination with antifungal drugs against mucormycosis agents","authors":"Fernando Almeida-Silva ,&nbsp;Pedro Henrique Tenório-Alvarenga ,&nbsp;Raiane Valle da Costa ,&nbsp;Rowena Alves Coelho ,&nbsp;Glauber Ribeiro de Sousa Araújo ,&nbsp;Rosely Maria Zancopé-Oliveira ,&nbsp;Susana Frases ,&nbsp;Rodrigo Almeida-Paes","doi":"10.1016/j.crmicr.2024.100242","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100242","url":null,"abstract":"<div><p>Mucormycosis is a severe fungal infection that demands immediate and decisive intervention upon suspicion. The causative agents of mucormycosis exhibit inherent resistance to echinocandins and voriconazole, and their in vitro susceptibility to terbinafine is highly variable and species-specific. Considering these factors and the limitations of currently available antifungal therapies, the identification of novel antifungals with potent activity against mucormycosis is of paramount importance. This study aims to identify compounds from the MMV Pathogen Box® presenting antifungal activity against selected mucormycosis agents and to evaluate their potential synergistic effects when combined with antifungal drugs. A screening of the Pathogen Box® compounds was conducted, isolated or in combination with sub-inhibitory concentrations of amphotericin B, isavuconazole or posaconazole, against a <em>Rhizopus oryzae</em> strain. Hits from the screenings were further evaluated against eight Mucoralean strains for minimal inhibitory and fungicidal concentration determinations and to confirm synergistic interactions using the checkerboard method. Ultrastructural studies were performed using scanning electron microscopy. MMV675968 exhibited fungicidal activity against a <em>R. oryzae</em> strain. All but one <em>Rhizopus</em> spp. strains presented MIC ≤ 1 μg/mL, with a geometric mean of 0.78 μg/mL observed across all isolates for this compound, which did not change significantly the cellular structure of this fungus. The combination screening with antifungal drugs revealed six additional compounds potentially active against the <em>R. oryzae</em> strain, two of them demonstrated proven synergism through the checkerboard assay. This first study with the MMV Pathogen Box® and Zigomycetes highlights promising new treatment options for mucormycosis in the future.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100242"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000245/pdfft?md5=b7e9368142aec1251e26fae43097a595&pid=1-s2.0-S2666517424000245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141068500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}