Current Research in Microbial Sciences最新文献

{"title":"Pseudomonas rossensis sp. nov., a novel psychrotolerant species produces antimicrobial agents targeting resistant clinical isolates of Pseudomonas aeruginosa","authors":"Kateřina Snopková ,&nbsp;Karel Sedlář ,&nbsp;Dana Nováková ,&nbsp;Eva Staňková ,&nbsp;Ivo Sedláček ,&nbsp;Ondřej Šedo ,&nbsp;Veronika Holá","doi":"10.1016/j.crmicr.2025.100353","DOIUrl":"10.1016/j.crmicr.2025.100353","url":null,"abstract":"<div><div>The extreme conditions of the Antarctic environment have driven the evolution of highly specialized microbial communities with unique adaptations. In this study, we characterized five <em>Pseudomonas</em> isolates from James Ross Island, which displayed notable taxonomic and metabolite features. Phylogenomic analysis revealed that strain P2663^T occupies a distinct phylogenetic position within the <em>Pseudomonas</em> genus, related to species <em>Pseudomonas svalbardensis, Pseudomonas silesiensis, Pseudomonas mucoides, Pseudomonas prosekii</em>, and <em>Pseudomonas gregormendelii</em>. The novelty of five Antarctic isolates was further confirmed through analyses of housekeeping genes, ribotyping, and REP-PCR profiling. MALDI-TOF MS analysis identified 11 unique mass spectrometry signals shared by the Antarctic isolates, which were not detected in other related species. Additionally, chemotaxonomic characterization, including fatty acid composition, demonstrated similarities with related <em>Pseudomonas</em> species. Phenotypic assessments revealed distinctive biochemical and physiological traits.</div><div>In-depth genomic analysis of strain P2663^T uncovered numerous genes which could be involved in survival in extreme Antarctic conditions, including those encoding cold-shock and heat-shock proteins, oxidative and osmotic stress response proteins, and carotenoid-like pigments. Genome mining further revealed several biosynthetic gene clusters, some of which are associated with antimicrobial activity. Functional assays supported the antimicrobial capabilities of this novel species, showing antagonistic effects against clinical isolates of <em>Pseudomonas aeruginosa</em>, possibly mediated by tailocins (phage tail-like particles).</div><div>This comprehensive polyphasic study characterized a new cold-adapted species, for which we propose the name <em>Pseudomonas rossensis</em> sp. nov.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100353"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of melioidosis and its associated risk factors –A population-based study in Odisha, Eastern India","authors":"Bijayini Behera ,&nbsp;Arvind K Singh ,&nbsp;Mohammad Ahmad ,&nbsp;Lipipuspa Rout ,&nbsp;Jayanti Jena ,&nbsp;Asmita Patnaik ,&nbsp;Pradeep Behera ,&nbsp;Payal Priyadarshini ,&nbsp;Dhruv Pandey ,&nbsp;Po-Lin Chan ,&nbsp;Biswa Prakash Dutta ,&nbsp;Prasanta R. Mohapatra ,&nbsp;Jitendriya Amrit Pritam ,&nbsp;Srujana Mohanty ,&nbsp;Ashoka Mahapatra ,&nbsp;Abhisek Mishra","doi":"10.1016/j.crmicr.2025.100360","DOIUrl":"10.1016/j.crmicr.2025.100360","url":null,"abstract":"<div><div>Melioidosis, caused by <em>Burkholderia pseudomallei</em>, is an emerging disease in Odisha, a state in eastern coastal India. Difficult to diagnose, the disease is likely to be severely underreported. Seroprevalence studies in the general population are deemed necessary for an estimate of melioidosis endemicity and to explore the associated risk factors. A population-based cross-sectional seroprevalence study was conducted using Indirect Hemagglutination Assay (IHA) among 1920 participants aged 5–60 years residing in six out of thirty districts of Odisha from August to December 2023. Seropositivity was defined as an IHA titer ≥ 1:20. The risk factors associated with seropositivity were determined. Out of 1920 individuals, 1215 (63.3 %) were females and 1680 (87.5 %) were rural residents. 410 out of 1920 individuals had IHA titer ≥ 1:20, contributing to an overall prevalence of 20.9 % [95 % CI: 19.0 % - 22.7 %]. Seropositivity was highest among those aged 21 to 30 years [23.2 % (95 % CI: 19.2 % - 27.4 %)], and in females [21.7 % (95 % CI: 19.4 % – 24.1 %)]. The odds of seropositivity were 1.64 [95 % CI: 1.097 - 2.436, p= 0.016] times higher among rural residents than urban residents with people engaged in cultivation, daily activities involving soil, and water bodies having higher odds of seropositivity (Crude Odds Ratios, 1.22, 1.11 and 1.17 respectively).The present study, covering a single post-monsoon season in Odisha, has yielded a 20.9 % melioidosis seropositivity, matching previous Indian studies with <em>B. pseudomallei</em> seropositivity rates ranging from 20–29 %. The study indicates towards widespread environmental presence of <em>B. pseudomallei</em>, more so in rural areas and thus risk of clinical melioidosis. Adoption of public health interventions as well creation of awareness is of paramount importance in such a scenario.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100360"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143422168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the role of the PhoP master regulator in arsenite resistance through ackA downregulation in Lacticaseibacillus paracasei","authors":"Daniela Corrales ,&nbsp;Cristina Alcántara ,&nbsp;Dinoraz Vélez ,&nbsp;Vicenta Devesa ,&nbsp;Vicente Monedero ,&nbsp;Manuel Zúñiga","doi":"10.1016/j.crmicr.2025.100357","DOIUrl":"10.1016/j.crmicr.2025.100357","url":null,"abstract":"<div><div>In bacteria, the two-component system PhoPR plays an important role in regulating many genes related to phosphate uptake and metabolism. In <em>Lacticaseibacillus paracasei</em> inactivation of the response regulator PhoP results in increased resistance to arsenite [As(III)]. A comparative transcriptomic analysis revealed that the absence of PhoP has a strong effect on the transcriptome, with about 57.5 % of <em>Lc. paracasei</em> genes being differentially expressed, although only 92 of the upregulated genes and 23 of the downregulated genes reached a fold change greater than 2. Among them, the <em>phnDCEB</em> cluster, encoding a putative ABC phosphonate transporter and the acetate kinase encoding gene <em>ackA</em> (LCABL_01600) were downregulated tenfold and sevenfold, respectively. In vitro binding assays with selected PhoP-regulated genes showed that phosphorylation of PhoP stimulated its binding to the promoter regions of <em>pstS</em> (phosphate ABC transporter binding subunit), <em>phnD</em> and <em>glnA</em> glutamine synthetase) whereas no binding to the <em>poxL</em> (pyruvate oxidase) or <em>ackA</em> putative promoter regions was detected. This result identified for the first time three genes/operons belonging to the Pho regulon in a <em>Lactobacillaceae</em> species. Mapping of the reads obtained in the transcriptomic analysis revealed that transcription of <em>ackA</em> was severely diminished in the PhoP mutant after a hairpin structure located within the <em>ackA</em> coding region. Inactivation of <em>phnD</em> did not affect As(III) resistance whereas inactivation of <em>ackA</em> resulted in the same level of resistance as that observed in the PhoP mutant. These finding strongly suggests that PhoP mutant As(III) resistance is due to downregulation of <em>ackA</em>. Possible mechanisms of action are discussed.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100357"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143387382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human microbiome in post-acute COVID-19 syndrome (PACS)","authors":"Arezoo Fallah ,&nbsp;Hamid Sedighian ,&nbsp;Reza Kachuei ,&nbsp;Abbas Ali Imani Fooladi","doi":"10.1016/j.crmicr.2024.100324","DOIUrl":"10.1016/j.crmicr.2024.100324","url":null,"abstract":"<div><div>The global COVID-19 pandemic, which began in 2019, is still ongoing. SARS-CoV-2, also known as the severe acute respiratory syndrome coronavirus 2, is the causative agent. Diarrhea, nausea, and vomiting are common GI symptoms observed in a significant number of COVID-19 patients. Additionally, the respiratory and GI tracts express high level of transmembrane protease serine 2 (TMPRSS2) and angiotensin-converting enzyme-2 (ACE2), making them primary sites for human microbiota and targets for SARS-CoV-2 infection. A growing body of research indicates that individuals with COVID-19 and post-acute COVID-19 syndrome (PACS) exhibit considerable alterations in their microbiome. In various human disorders, including diabetes, obesity, cancer, ulcerative colitis, Crohn's disease, and several viral infections, the microbiota play a significant immunomodulatory role.</div><div>In this review, we investigate the potential therapeutic implications of the interactions between host microbiota and COVID-19. Microbiota-derived metabolites and components serve as primary mediators of microbiota-host interactions, influencing host immunity. We discuss the various mechanisms through which these metabolites or components produced by the microbiota impact the host's immune response to SARS-CoV-2 infection. Additionally, we address confounding factors in microbiome studies. Finally, we examine and discuss about a range of potential microbiota-based prophylactic measures and treatments for COVID-19 and PACS, as well as their effects on clinical outcomes and disease severity.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100324"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The spatial mapping of melioidosis exposure in the eastern Indian state of Odisha","authors":"TS Sarin ,&nbsp;V. Vinoj ,&nbsp;Gopika Pragna Gujjula ,&nbsp;Bijayini Behera ,&nbsp;Jayanti Jena ,&nbsp;Srujana Mohanty","doi":"10.1016/j.crmicr.2025.100346","DOIUrl":"10.1016/j.crmicr.2025.100346","url":null,"abstract":"<div><div>Melioidosis, a potentially fatal yet treatable disease complex caused by environmental Gram-negative saprophyte <em>Burkholderia pseudomallei,</em> is an ignored and neglected clinical entity. Excluding the hyperendemic regions of Thailand and Australia, the Indian subcontinent is among the areas with the highest predicted global burden of this disease. Melioidosis is not a notifiable disease in India, and in the last decade, most of the published cases have come from western coastal India and Odisha. The extent of the prevalence of melioidosis and its relationship with various climatic elements need to be better understood, and only a small number of studies have focused on it. Given the mortality of melioidosis, which is around 50 % worldwide, the identification of vulnerable locations is crucial for government entities and policymakers for the effective management and mitigation of the disease. In numerous studies, the prevalence of melioidosis is significantly linked to weather parameters such as rainfall, soil temperature, air temperature, soil moisture, and solar radiation. In this study, using hospital records of the disease and weather parameters from reanalysis datasets, we estimated the potential exposure of different regions in Odisha, a state in eastern India, a leading recorder of melioidosis cases annually. Our analysis reveals that the disease exhibits a clear seasonality, with most cases occurring during the monsoon season (higher by a factor of two compared to other seasons). Disease occurrence also exhibits relationships with temperature, rainfall, cloud cover and solar radiation. Analysis using these variables on disease exposure reveals that all regions of Odisha are vulnerable to melioidosis to variable degrees, out of which the districts of Cuttack, Balasore, Khordha and Jajpur are most vulnerable. The results obtained are expected to increase awareness regarding the potential risk of the issue and help government agencies develop effective mitigation measures.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100346"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The multidrug-resistant Candida auris, Candida haemulonii complex and phylogenetic related species: Insights into antifungal resistance mechanisms","authors":"Lívia S. Ramos ,&nbsp;Pedro F. Barbosa ,&nbsp;Carolline M.A. Lorentino ,&nbsp;Joice C. Lima ,&nbsp;Antonio L. Braga ,&nbsp;Raquel V. Lima ,&nbsp;Lucas Giovanini ,&nbsp;Ana Lúcia Casemiro ,&nbsp;Nahyara L.M. Siqueira ,&nbsp;Stefanie C. Costa ,&nbsp;Célia F. Rodrigues ,&nbsp;Maryam Roudbary ,&nbsp;Marta H. Branquinha ,&nbsp;André L.S. Santos","doi":"10.1016/j.crmicr.2025.100354","DOIUrl":"10.1016/j.crmicr.2025.100354","url":null,"abstract":"<div><div>The rise of multidrug-resistant (MDR) fungal pathogens poses a serious global threat to human health. Of particular concern are <em>Candida auris</em>, the <em>Candida haemulonii</em> complex (which includes C<em>. haemulonii sensu stricto, C. duobushaemulonii</em> and <em>C. haemulonii</em> var. <em>vulnera</em>), and phylogenetically related species, including <em>C. pseudohaemulonii</em> and <em>C. vulturna</em>. These emerging, widespread, and opportunistic pathogens have drawn significant attention due to their reduced susceptibility to commonly used antifungal agents, particularly azoles and polyenes, and, in some cases, therapy-induced resistance to echinocandins. Notably, <em>C. auris</em> is classified in the critical priority group on the World Health Organization's fungal priority pathogens list, which highlights fungal species capable of causing systemic infections with significant mortality and morbidity risks as well as the challenges posed by their MDR profiles, limited treatment and management options. The mechanisms underlying antifungal resistance within these emerging fungal species is still being explored, but some advances have been achieved in the past few years. In this review, we compile current literature on the distribution of susceptible and resistant clinical strains of <em>C. auris, C. haemulonii</em> complex, <em>C. pseudohaemulonii</em> and <em>C. vulturna</em> across various antifungal classes, including azoles (fluconazole, voriconazole, itraconazole), polyenes (amphotericin B), echinocandins (caspofungin, micafungin, anidulafungin), and pyrimidine analogues (flucytosine). We also outline the main antifungal resistance mechanisms identified in planktonic cells of these yeast species. Finally, we explore the impact of biofilm formation, a classical virulence attribute of fungi, on antifungal resistance, highlighting the resistance mechanisms associated with this complex microbial structure that have been uncovered to date.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100354"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of antifungal therapy: Traditional agents, current challenges and future perspectives","authors":"Cássia Milena de Souza ,&nbsp;Bárbara Tavares Bezerra ,&nbsp;Daniel Agreda Mellon ,&nbsp;Haroldo Cesar de Oliveira","doi":"10.1016/j.crmicr.2025.100341","DOIUrl":"10.1016/j.crmicr.2025.100341","url":null,"abstract":"<div><div>Fungal infections kill more than 3 million people every year. This high number reflects the significant challenges that treating these diseases worldwide presents. The current arsenal of antifungal drugs is limited and often accompanied by high toxicity to patients, elevated treatment costs, increased frequency of resistance rates, and the emergence of naturally resistant species. These treatment challenges highlight the urgency of developing new antifungal therapies, which could positively impact millions of lives each year globally. Our review offers an overview of the antifungal drugs currently available for treatment, presents the status of new antifungal drugs under clinical study, and explores ahead to future candidates that aim to help address this important global health issue.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100341"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida","authors":"Rongchao He ,&nbsp;Yanfei Zuo ,&nbsp;Qiu Li ,&nbsp;Qingpi Yan ,&nbsp;Lixing Huang","doi":"10.1016/j.crmicr.2025.100351","DOIUrl":"10.1016/j.crmicr.2025.100351","url":null,"abstract":"<div><div>LexA is a well-known transcriptional repressor of DNA repair genes induced by DNA damage in <em>Escherichia coli</em> and other bacterial species. Recently, this paradigm—that LexA solely regulates the SOS response—has been challenged as studies reveal its involvement in various biological functions linked to virulence. <em>Pseudomonas plecoglossicida</em>, a major pathogen in mariculture, causes substantial economic losses annually in China. Our previous research suggested that LexA might collaboratively regulate virulence gene expression with HtpG during infection. This study aims to elucidate the molecular mechanism by which LexA controls virulence gene expression. We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. Our findings identified 36 downstream virulence genes regulated by LexA, define three critical LexA binding motifs, and provide an in-depth analysis of LexA's recognition and binding to promoters, thereby regulating virulence gene expression. Additionally, we confirm the cooperative regulatory roles of HtpG, RecA, and LexA in virulence gene modulation. This is the first report of an endogenous accessory factor aiding in the binding of LexA to DNA. This study enhances our understanding of LexA's role in virulence regulation and offers a valuable theoretical and practical foundation for disease prevention and control.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100351"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana","authors":"Panpan Xue ,&nbsp;Le Xu ,&nbsp;Yakai Tian ,&nbsp;Mingkui Lv ,&nbsp;Pingping Fang ,&nbsp;Kun Dong ,&nbsp;Qiuye Lin ,&nbsp;Zhenhui Cao","doi":"10.1016/j.crmicr.2025.100343","DOIUrl":"10.1016/j.crmicr.2025.100343","url":null,"abstract":"<div><div>While the health benefits of lactic acid bacteria (LAB)-fermented feed on farmed animals are well-established, its potential benefits for honeybees, specifically <em>Apis cerana cerana</em>, remain largely unexplored. The present study aimed to optimize an enzymatic hydrolysis process for tea bee pollen, employing a complex enzyme comprising acid cellulase and pectinase, followed by fermentation with <em>Limosilactobacillus reuteri</em> LP4. <em>A. c. cerana</em> workers were subsequently fed tea bee pollen processed with this optimized method. Under the optimal processing condition of fermented tea bee pollen, the pH value was 4.41, the protein content was 27.75 %, and the viable count of LAB was 2.31×10⁹ CFU/g. No molds and yeasts as well as pathogens were detected. Compared to the unfermented pollen, honey bee workers administrated with fermented tea pollen with L. <em>reuteri</em> LP4 showed significantly increased survival rate by 24.34 % on day 15. Moreover, the relative abundances of <em>Lactobacillus</em> and <em>Bifidobacterium</em> were elevated, while those of <em>Enterococcus</em> and <em>Bacteroides</em> were diminished. Concurrently, the relative expression levels of immune-related genes including <em>Abaecin, PPO, Defensin</em>, and <em>Vg</em> were significantly upregulated. These findings provide a scientific foundation for application of fermented feeds to enhance the health of <em>A. c. cerana</em> populations and contribute to the sustainable development of apiculture in China.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100343"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel mode of histone-like protein HupB regulating Sinorhizobium meliloti cell division through lysine acetylation","authors":"Ningning Li ,&nbsp;Huibo Jin ,&nbsp;Hongbo Li ,&nbsp;Huilin Yu ,&nbsp;Xiaoxu Wu ,&nbsp;Tianci Zhang ,&nbsp;Liangliang Yu ,&nbsp;Zhaoling Qin ,&nbsp;Li Luo","doi":"10.1016/j.crmicr.2025.100345","DOIUrl":"10.1016/j.crmicr.2025.100345","url":null,"abstract":"<div><div>HU, a small, basic histone-like protein, binds to bacterial genomic DNA, influencing DNA conformation, replication, and transcription. Its acetylation is a key post-translational modification affecting its DNA-binding activity. The role of HU acetylation in regulating cell division through the cell cycle regulatory system remained largely unknown. In this study, we find that stimulation of lysine acetylation or non-acetylation in HupB, a homolog of HU, differentially regulates the expression of cell cycle regulators, as well as cell growth and division in <em>Sinorhizobium meliloti</em>. Lys3, Lys13, and Lys83 in HupB were identified as acetylated residues by mass spectrometry. Mutating these residues to arginine (stimulating non-acetylation) in HupB impedes normal cell division, while substituting them with glycine (mimicking acetylation) allows for rapid cell duplication. The mimicry of non-acetylated HupB leads to enlarged abnormal cells, while stimulating acetylated HupB only reduces cell length. Transcription activation was observed in the mutant cells. Cell cycle regulators such as CtrA, GcrA and DnaA were differentially expressed in the mutants. HupB substitutions differentially bound to these cell cycle regulatory genes. These findings suggest that the appropriate acetylation of HupB regulates the expression of cell cycle regulators, thereby controlling <em>S. meliloti</em> cell division.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100345"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}