Current Research in Microbial Sciences最新文献

{"title":"Accelerating the understanding of Aspergillus terreus: Epidemiology, physiology, immunology and advances","authors":"Raman Thakur ,&nbsp;Sonia Kumari Shishodia ,&nbsp;Ananya Sharma ,&nbsp;Arjun Chauhan ,&nbsp;Sumanpreet Kaur ,&nbsp;Jata Shankar","doi":"10.1016/j.crmicr.2024.100220","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100220","url":null,"abstract":"<div><p><em>Aspergillus</em> species encompass a variety of infections, ranging from invasive aspergillosis to allergic conditions, contingent upon the immune status of the host. In this spectrum, <em>Aspergillus terreus</em> stands out due to its emergence as a notable pathogen and its intrinsic resistance to amphotericin-B. The significance of <em>Aspergillus</em>-associated infections has witnessed a marked increase in the past few decades, particularly with the increasing number of immunocompromised individuals. The exploration of epidemiology, morphological transitions, immunopathology, and novel treatment approaches such as new antifungal drugs (PC945, olorofim) and combinational therapy using antifungal drugs and phytochemicals (Phytochemicals: quercetin, shikonin, artemisinin), also using immunotherapies to modulate immune response has resulted in better outcomes. Furthermore, in the context COVID-19 era and its aftermath, fungal infections have emerged as a substantial challenge for both immunocompromised and immunocompetent individuals. This is attributed to the use of immune-suppressing therapies during COVID-19 infections and the increase in transplant cases. Consequently, this review aims to provide an updated overview encompassing the epidemiology, germination events, immunopathology, and novel drug treatment strategies against <em>Aspergillus terreus-</em>associated infections.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100220"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000026/pdfft?md5=ede7f4424c3009102739c9f6f7deaf29&pid=1-s2.0-S2666517424000026-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The search for an antiviral lead molecule to combat the neglected emerging Oropouche virus","authors":"Rafaela dos Santos Peinado ,&nbsp;Marielena Vogel Saivish ,&nbsp;Gabriela de Lima Menezes ,&nbsp;Umberto Laino Fulco ,&nbsp;Roosevelt Alves da Silva ,&nbsp;Karolina Korostov ,&nbsp;Raphael Josef Eberle ,&nbsp;Paulo A. Melo ,&nbsp;Maurício Lacerda Nogueira ,&nbsp;Carolina Colombelli Pacca ,&nbsp;Raghuvir Krishnaswamy Arni ,&nbsp;Mônika Aparecida Coronado","doi":"10.1016/j.crmicr.2024.100238","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100238","url":null,"abstract":"<div><p>Oropouche virus (OROV) is a member of the <em>Peribunyaviridae</em> family and the causative agent of a dengue-like febrile illness transmitted by mosquitoes. Although mild symptoms generally occur, complications such as encephalitis and meningitis may develop. A lack of proper diagnosis, makes it a potential candidate for new epidemics and outbreaks like other known arboviruses such as Dengue, Yellow Fever and Zika virus. The study of natural molecules as potential antiviral compounds is a promising alternative for antiviral therapies. Wedelolactone (WDL) has been demonstrated to inhibit some viral proteins and virus replication, making it useful to target a wide range of viruses. In this study, we report the <em>in silico</em> effects of WDL on the OROV N-terminal polymerase and its potential inhibitory effects on several steps of viral infection in mammalian cells <em>in vitro</em>, which revealed that WDL indeed acts as a potential inhibitor molecule against OROV infection.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100238"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000208/pdfft?md5=6a565bd97ce9ada55b6058d5c19c4e96&pid=1-s2.0-S2666517424000208-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide","authors":"Imen Nouioui ,&nbsp;Judith Boldt ,&nbsp;Alina Zimmermann ,&nbsp;Roman Makitrynskyy ,&nbsp;Gabriele Pötter ,&nbsp;Marlen Jando ,&nbsp;Meike Döppner ,&nbsp;Sarah Kirstein ,&nbsp;Meina Neumann-Schaal ,&nbsp;Juan Pablo Gomez-Escribano ,&nbsp;Ulrich Nübel ,&nbsp;Yvonne Mast","doi":"10.1016/j.crmicr.2024.100290","DOIUrl":"10.1016/j.crmicr.2024.100290","url":null,"abstract":"<div><div>Actinomycetes are a prolific source of bioactive natural compounds many of which are used as antibiotics or other drugs. In this study we investigated the genomic and biochemical diversity of 32 actinobacterial strains that had been deposited at the DSMZ–German Collection of Microorganisms and Cell Cultures decades ago. Genome-based phylogeny and <em>in silico</em> DNA-DNA hybridization supported the assignment of these strains to 26 novel species and two novel subspecies and a reclassification of a <em>Streptomyces</em> species. These results were consistent with the biochemical, enzymatic, and chemotaxonomic features of the strains. Most of the strains showed antimicrobial activities against a range of Gram-positive and Gram-negative bacteria, and against yeast. Genomic analysis revealed the presence of numerous unique biosynthetic gene clusters (BGCs) encoding for potential novel antibiotic and anti-cancer compounds. Strains DSM 41636^T and DSM 61640^T produced the antibiotic compounds A33853 and SF2768, respectively. Overall, this reflects the significant pharmaceutical and biotechnological potential of the proposed novel type strains and underlines the role of prokaryotic systematics for drug discovery. In order to compensate for the gender gap in naming prokaryotic species, we propose the eponyms for all newly described species to honour female scientists.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100290"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the unique roles of extracellular vesicles for gut health modulation: Mechanisms, challenges, and perspectives","authors":"Qiming Wu ,&nbsp;Juntao Kan ,&nbsp;Caili Fu ,&nbsp;Xin Liu ,&nbsp;Zhengying Cui ,&nbsp;Sixu Wang ,&nbsp;Yi Le ,&nbsp;Zhanming Li ,&nbsp;Qin Liu ,&nbsp;Yuyu Zhang ,&nbsp;Jun Du","doi":"10.1016/j.crmicr.2024.100301","DOIUrl":"10.1016/j.crmicr.2024.100301","url":null,"abstract":"<div><div>Extracellular vesicles (EVs), which play significant regulatory roles in maintaining homeostasis and influencing immune responses, significantly impact gut microbiota composition and function, affecting overall gut health. Despite considerable progress, there are still knowledge gaps regarding the mechanisms by which EVs, including plant-derived EVs (PDEVs), animal-derived EVs (ADEVs), and microbiota-derived EVs (MDEVs), modulate gut health. This review delves into the roles and mechanisms of EVs from diverse sources in regulating gut health, focusing on their contributions to maintaining epithelial barrier integrity, facilitating tissue healing, eliciting immune responses, controlling pathogens, and shaping microbiota. We emphasize open challenges and future perspectives for harnessing EVs in the modulation of gut health to gain a deeper understanding of their roles and impact. Importantly, a comprehensive research framework is presented to steer future investigations into the roles and implications of EVs on gut health, facilitating a more profound comprehension of this emerging field.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100301"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drought stress mitigation through bioengineering of microbes and crop varieties for sustainable agriculture and food security","authors":"Satish Kumar,&nbsp;Satyavir Singh Sindhu","doi":"10.1016/j.crmicr.2024.100285","DOIUrl":"10.1016/j.crmicr.2024.100285","url":null,"abstract":"<div><div>Climate change and agriculture are intrinsically connected and sudden changes in climatic conditions adversely impact global food production and security. The climate change-linked abiotic stressors like drought and high temperatures are resulting in crop failure. The most severe abiotic stress drought significantly affect the stomatal closure, production of reactive oxygen species, transpiration, photosynthesis or other physiological processes and plant morphology, and adversely affect plant growth and crop yield. Therefore, there is an exigent need for cost effective and eco-friendly modern technologies to induce drought tolerance in crop plants leading to climate-adapted sustainable agricultural practices for sustained food production. Among many options being pursued in this regard, the use of plant growth promoting microbes (PGPMs) is the most sustainable approach to promote drought stress resilience in crop plants leading to better plant growth and crop productivity. These PGPMs confer drought resistance via various direct or indirect mechanisms including production of antioxidants, enzymes, exopolysaccharides, modulation of phytohormones level, osmotic adjustment by inducing the accumulation of sugars, along with increases in nutrients, water uptake and photosynthetic pigments. However, several technological and ecological challenges limit their use in agriculture and sometimes treatment with plant beneficial microbes fails to produce desired results under field conditions. Thus, development of synthetic microbial communities or host mediated microbiome engineering or development of transgenic plants with the capacity to express desired traits may promote plant survival and growth under drought stress conditions. The present review critically assesses research evidence on the plant growth and stress resilience promoting potentials of PGPMs and their genes as an approach to develop drought resilient plants leading to increased crop productivity. Effective collaboration among scientific communities, policymakers and regulatory agencies is needed to create strong frameworks that both promote and regulate the utilization of synthetic microbial communities and transgenic plants in agriculture.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100285"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142531920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical review on the biotechnological potential of Brewers’ waste: Challenges and future alternatives","authors":"Sourav Chattaraj ,&nbsp;Debasis Mitra ,&nbsp;Arindam Ganguly ,&nbsp;Hrudayanath Thatoi ,&nbsp;Pradeep K. Das Mohapatra","doi":"10.1016/j.crmicr.2024.100228","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100228","url":null,"abstract":"<div><p>In order to comply with the stringent discharge guidelines issued by governmental organizations to protect the ecosystem, the substantial amounts of effluent and sturdy wastes produced by the beer brewing process need to be discarded or handled in the most affordable and secure manner. Huge quantities of waste material released with each brew bestow a significant opportunity for the brewing sector to move towards sustainability. The concept of circular economy and the development of technological advancements in brewery waste processing have spurred interest to valorize brewery waste for implementation in various sectors of medical and food science, industrial science, and many more intriguing fields. Biotechnological methods for valorizing brewery wastes are showing a path towards green chemistry and are feasible and advantageous to environment. The study unfolds most recent prospectus for brewery waste usage and discusses major challenges with brewery waste treatment and valorization and offers suggestions for further work.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100228"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000105/pdfft?md5=78f42849d77ba382a439477219af6a17&pid=1-s2.0-S2666517424000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140016134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus","authors":"P.Suresh Yadav ,&nbsp;B.V.Siva Prasad ,&nbsp;M.Subhosh Chandra ,&nbsp;M. Srinivasulu ,&nbsp;Naga Raju Maddela ,&nbsp;Ram Prasad","doi":"10.1016/j.crmicr.2024.100254","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100254","url":null,"abstract":"<div><p>Recovery of enzymes such as FPase (filter paperase) or exoglucanase from fermented substrate is a sustainable approach in enzyme production; however, there is a scarcity of optimization studies in this field. The present study was aimed to standardize number of parameters (selection of solvent, solvent volume, soaking time, leaching conditions and number of washes) to extract maximum amount of FPase from fermented rice husk by <em>Aspergillus protuberus.</em> Novel <em>Aspergillus protuberus</em> was first report from our lab on cellulases production in solid state fermentation (SSF). Among the tested solvents, citrate phosphate buffer (0.02 M, pH 5.0) proved best solvent for maximum recovery of FPase. Consequent experimental parameters were further optimized with citrate phosphate buffer. Two washes with citrate phosphate buffer each by shaking (60 min) in a ratio of 1 g of rice husk: 5 ml of citrate phosphate buffer together attained higher recovery efficiency (88 %) of FPase from the fermented rice husk.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100254"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000361/pdfft?md5=60c233f082116f9525bd7c066b2353e2&pid=1-s2.0-S2666517424000361-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141582476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidative stress and culture atmosphere effects on bioactive compounds and laccase activity in the white rot fungus Phlebia radiata on birch wood substrate","authors":"Eero Kiviniemi,&nbsp;Arttu Mikkola,&nbsp;Hans Mattila ,&nbsp;Matti Wahlsten,&nbsp;Taina Lundell","doi":"10.1016/j.crmicr.2024.100280","DOIUrl":"10.1016/j.crmicr.2024.100280","url":null,"abstract":"<div><div>Wood-decaying white rot fungi live in changing environmental conditions and may switch from aerobic to fermentative metabolism under oxygen depletion. Decomposition of wood and lignocellulose by fungi is dependent on enzymatic and oxidative biochemistry including generation of reactive oxygen species. In this study, we subjected semi-solid wood-substrate cultures of the white rot fungus <em>Phlebia radiata</em> to oxidative stress by addition of hydrogen peroxide under aerobic and anaerobic cultivation conditions. Wood decomposition and fungal metabolism were followed by analysis of extracellular organic compounds, mycelial growth, and laccase activity. Under both atmospheric conditions, accumulation of bioactive aromatic compounds from birch wood occurred into the culture supernatants after hydrogen peroxide treatment. The supernatants inhibited both fungal growth and laccase activity. However, the fungus recovered from the oxidative stress quickly in a few days, especially when cultivated under regular aerobic conditions. With repeated hydrogen peroxide treatments, laccase suppressive-recovery effect was observed. Culture supernatants demonstrated antioxidant and antimicrobial effects, in concert with emergence of chlorinated birch-derived organic compounds. Bioactivities in the cultures disappeared in the same pace as the chlorinated compounds were transformed and de-chlorinated by the fungus. Our results indicate tolerance of white rot fungi against excessive oxidative stress and wood-derived, growth-inhibiting and harmful agents.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100280"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142359458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quorum quenching activity of endophytic Bacillus sp. EBS9 from Tecomella undulata and its biocontrol applications","authors":"Etisha Paul,&nbsp;Charu Sharma,&nbsp;Payal Chaturvedi,&nbsp;Pradeep Bhatnagar","doi":"10.1016/j.crmicr.2024.100307","DOIUrl":"10.1016/j.crmicr.2024.100307","url":null,"abstract":"<div><div>This study investigates the quorum quenching (QQ) activity of an endophytic bacterium, <em>Bacillus</em> sp. EBS9, isolated from the native medicinal plant <em>Tecomella undulata</em> of Rajasthan, and its biocontrol potential against the soft rot pathogen <em>Pectobacterium carotovorum</em> subsp. <em>carotovorum</em> (<em>Pcc</em>). QQ activity was confirmed by the loss of violacein pigment in <em>Chromobacterium violaceum</em> (MCC 2290). Quorum quenching metabolites were extracted using ethyl acetate, and the Quorum Quenching Extract (QQE) demonstrated positive activity in assays with <em>C. violaceum</em> CV026 (MCC 2216). HRLC-MS analysis identified diketopiperazines, L,L-Cyclo (leucylprolyl) and Cyclo (L-Phe-L-Pro), which are N-acyl homoserine lactones (AHLs) antagonists competing for LuxR receptor binding sites. In vitro and <em>in planta</em> assays evaluated QQB's biocontrol potential using treatment I (<em>Pcc</em>), treatment II (<em>Pcc</em> + QQB), and a control (sterile water). In the in vitro soft rot attenuation assay showed that treatment I caused severe maceration in vegetable slices, particularly in radish, exhibiting the highest maceration diameter (25.33 ± 3.52 mm) and percentage (46.14 ± 5.70 %). However, co-inoculation with QQB significantly reduced maceration across all tested vegetables. In the plate assay, germination rates decreased to approximately 50 % in both <em>Vigna radiata</em> and <em>Raphanus sativus</em> for treatment I, but improved to 86.67 % in treatment II. The seed vigour and germination indices also improved with QQB treatment in both plant species. In the pot assay after 30 days, in contrast to a 50 % decrease in root and shoot lengths in treatment I, treatment II led to a substantial recovery, with root lengths increase by 112.07 % and 138.76 %, while shoot length by 315.65 % and 163.63 % in <em>V. radiata</em> and <em>R. sativus</em>, respectively. This study highlights the QQ and biocontrol potential of <em>Bacillus</em> sp. EBS9 against <em>P. carotovorum</em> (<em>Pcc</em>), suggesting its promise in effective management of phytopathogens, which is crucial for agricultural productivity while minimizing environmental impact.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"7 ","pages":"Article 100307"},"PeriodicalIF":4.8,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142661848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into population adaptation and biodiversity of lactic acid bacteria in challenged date palm leaves silaging, using MALDI–TOF MS","authors":"Muhammad Zaid Jawaid,&nbsp;Mohammad Yousaf Ashfaq,&nbsp;Mohammad Al-Ghouti,&nbsp;Nabil Zouari","doi":"10.1016/j.crmicr.2024.100235","DOIUrl":"https://doi.org/10.1016/j.crmicr.2024.100235","url":null,"abstract":"<div><p>The study focused on isolating indigenous Qatari lactic acid bacteria (LAB) from various challenged date palm tree leaf silages to construct a comprehensive strain collection, useful to study the diversity of these strains following their adaptation to the uncommon silage. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI–TOF MS) was employed for strain identification and differentiation. The diversity of LAB populations and strains was assessed through principal component analysis (PCA) and dendrogram analyses. A total of 88 LAB isolates were obtained from silages of fresh palm leaves, silage of mixed leaves and dairy feed, along with fresh palm tree leaves, and dairy feed, adapted to local harsh environments. These isolates were categorized according to the new classification of 2020, belonging to genera of <em>Pediococcus, Lactiplantibacillus plantarum, Lacticaseibacillus paracasei, Companilactobacillus farciminis, Limosilactobacillus oris, Limosilactobacillus vaginalis, Lactiplantibacillus pentosus</em> and <em>Lactobacillus johnsonii. Pediococcus</em> was the most prevalent genus, falling mostly within the species <em>Pediococcus lolii</em>. MALDI–TOF MS protein profiles, PCA, and dendrogram analyses successfully grouped the LAB isolates into five distinctive clusters based on the protein's similarities. The high diversity of the indigenous LAB in spontaneous palm leaf silages demonstrated their adaptation and mutualistic interactions, forming robust consortia that ensure the quality of the silage. The straightforward, quick, and accurate identification of LAB in this silage using MALDI–TOF MS presents a valuable approach for formulating LAB consortia for silaging harsh agricultural by-products.</p></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"6 ","pages":"Article 100235"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666517424000178/pdfft?md5=42956d076b3b4bab2a3a1d2fac807a90&pid=1-s2.0-S2666517424000178-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140605265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}