Current Research in Microbial Sciences最新文献

{"title":"Assessing cyanobacterial spatial distribution in reservoirs using acoustic survey techniques for enhanced water quality management","authors":"Taisei Sugawara ,&nbsp;Kenji Minami ,&nbsp;Sangyeob Kim ,&nbsp;Takashi Inagawa ,&nbsp;Jiro Okitsu ,&nbsp;Shougo Sakamoto ,&nbsp;Tomonori Osugi ,&nbsp;Nobuyuki Azuma ,&nbsp;Yanhui Zhu ,&nbsp;Kazushi Miyashita","doi":"10.1016/j.crmicr.2025.100477","DOIUrl":"10.1016/j.crmicr.2025.100477","url":null,"abstract":"<div><div>Increases in water temperature due to climate change are expected to lead to cyanobacterial blooms in various freshwater bodies, including reservoirs, posing a major challenge in terms of water quality management. Therefore, a detailed understanding of the dynamics of cyanobacteria in water bodies is required to increase our knowledge of cyanobacteria. In this study, we attempted to determine the spatial distribution of cyanobacteria in a water body by acoustic techniques, and to understand the distribution of cyanobacteria and how it is affected by the water environment. At the same time, the validity of acoustic measurements of cyanobacteria was verified. Comparison of the estimated abundance of cyanobacteria by acoustic measurements and the measured pigment values of cyanobacteria showed a positive correlation between them, suggesting that acoustic measurements of cyanobacteria are effective. The field survey provided us with a large number of data and allowed us to map the spatial distribution of cyanobacteria in the water body. The results suggest that cyanobacteria are propagated or transported by the water quality control facilities of the reservoir and the water environment. In particular, unlike natural environments, reservoirs are expected to undergo severe environmental changes due to artificial facilities such as water quality control measures, and it will be important to continue monitoring cyanobacteria on a detailed scale using acoustic techniques. Continued research in this area is anticipated to lead to more effective strategies for managing water bodies and mitigating cyanobacterial blooms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100477"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145219095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial potential and phytochemical analysis of two ethnomedicinally important plants","authors":"Narpat Singh,&nbsp;Neha Chhangani,&nbsp;Sharad Bissa","doi":"10.1016/j.crmicr.2024.100297","DOIUrl":"10.1016/j.crmicr.2024.100297","url":null,"abstract":"<div><div>Medicinal plants exhibited great role in drug industries. Herbal medicines and their derivative products are often prepared from crude plant extracts. <em>Echinops echinatus</em> and <em>Tridax procumbens</em> both are belonging to Asteraceae family and these plants are ethnomedicinally important due to their utilization as traditional medicine to cure various diseases. Aim of the current study is to evaluate the antimicrobial properties, preliminary phytochemical and GC-MS analysis of these ethnomedicinally important plants to identify the compounds which are responsible for antimicrobial properties. Their extracts exhibited antimicrobial activity against <em>Enterobacter aerogenes, Escherichia coli, Agrobacterium tumefaciens, Staphylococcus aureus, Bacillus subtilis, Pseudomonas syringae</em> and <em>Pseudomonas putida</em>. Both plants contain the active principles like flavonoids, alkaloids, glycosides, saponins, terpenoids and tannins. Result of GC-MS analysis showed the presence of many compounds such as n-Hexadecanoic acid, Hexadecanoic Acid, Methyl ester, Octadecanoic acid, Stigmasterol, Naphthalene, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-, Squalene, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-,5 Hydroxymethylfurfural, Lupeol, Dodecanoic acid, Vitamin E (α-Tocopherol), Neophytadiene, Phytol and many other compounds. These compounds are responsible for antimicrobial, anticancer and medicinal properties.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100297"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11681831/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative genomics of Aspergillus nidulans and section Nidulantes","authors":"Sebastian Theobald ,&nbsp;Tammi Vesth ,&nbsp;Jane L. Nybo ,&nbsp;Jens C. Frisvad ,&nbsp;Inge Kjærbølling ,&nbsp;Stephen Mondo ,&nbsp;Kurt LaButti ,&nbsp;Sajeet Haridas ,&nbsp;Robert Riley ,&nbsp;Alan A. Kuo ,&nbsp;Asaf A. Salamov ,&nbsp;Jasmyn Pangilinan ,&nbsp;Anna Lipzen ,&nbsp;Maxim Koriabine ,&nbsp;Mi Yan ,&nbsp;Kerrie Barry ,&nbsp;Alicia Clum ,&nbsp;Ellen K. Lyhne ,&nbsp;Elodie Drula ,&nbsp;Ad Wiebenga ,&nbsp;Mikael R. Andersen","doi":"10.1016/j.crmicr.2025.100342","DOIUrl":"10.1016/j.crmicr.2025.100342","url":null,"abstract":"<div><div><em>Aspergillus nidulans</em> is an important model organism for eukaryotic biology and the reference for the section <em>Nidulantes</em> in comparative studies. In this study, we <em>de novo</em> sequenced the genomes of 25 species of this section. Whole-genome phylogeny of 34 <em>Aspergillus</em> species and <em>Penicillium chrysogenum</em> clarifies the position of clades inside section <em>Nidulantes</em>. Comparative genomics reveals a high genetic diversity between species with 684 up to 2433 unique protein families. Furthermore, we categorized 2118 secondary metabolite gene clusters (SMGC) into 603 families across Aspergilli, with at least 40 % of the families shared between <em>Nidulantes</em> species. Genetic dereplication of SMGC and subsequent synteny analysis provides evidence for horizontal gene transfer of a SMGC. Proteins that have been investigated in <em>A. nidulans</em> as well as its SMGC families are generally present in the section <em>Nidulantes</em>, supporting its role as model organism. The set of genes encoding plant biomass-related CAZymes is highly conserved in section <em>Nidulantes</em>, while there is remarkable diversity of organization of MAT-<em>loci</em> both within and between the different clades. This study provides a deeper understanding of the genomic conservation and diversity of this section and supports the position of <em>A. nidulans</em> as a reference species for cell biology.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100342"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11787670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity of single-celled microorganism Blastocystis sp. and its associated gut microbiome in free-ranging marine mammals from North-Western Mediterranean Sea","authors":"Marianna Marangi ,&nbsp;Sonia Boughattas","doi":"10.1016/j.crmicr.2025.100349","DOIUrl":"10.1016/j.crmicr.2025.100349","url":null,"abstract":"<div><div><em>Blastocystis</em> sp. is frequently identified in humans and several animal hosts exhibiting a wide genetic diversity. Within One Health perspective, data on <em>Blastocystis</em> sp. distribution and its circulating subtypes (STs) from the terrestrial environment are available, while those from the marine environment remain still scare. A genetic and 16S rRNA gene sequencing analysis were conducted over the period 2022–2024 by screening fecal samples from four different species of free-ranging marine mammals (sperm, fin, long-finned pilot and Cuvier's beaked whales) circulating within North-Western Mediterranean Sea. 10 out of 43 fecal samples (23.2 %) were found positive to <em>Blastocystis</em> sp. using molecular tools. A predominance of zoonotic subtype ST3 among different species of marine mammals as well as the presence of ST1 allele 4 subtype and even untypable subtype within the fin whale specimen was reported. Moreover, Firmicutes, Bacteroidetes and Proteobacteria within the different <em>Blastocystis</em>-carrier marine mammal species as well the identification of Archaebacteria from Methanomethylophilaceae family within the fin whale isolate were detected by Illumina V3-V4 generated data. The present survey presents new insights regarding <em>Blastocystis</em> sp. prevalence and its circulating zoonotic ST1-ST3 subtypes from the marine environment, as well as its associated gut microbiome, providing hence baseline data for a better understanding of the associated risk and to prevent human and marine ecosystem exposure to these anthropogenic microorganisms.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100349"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The endophytic fungus Cosmosporella sp. VM-42 from Vinca minor is a source of bioactive compounds with potent activity against drug-resistant bacteria","authors":"Ting He ,&nbsp;Xiao Li ,&nbsp;Rosario del Carmen Flores-Vallejo ,&nbsp;Ana-Maria Radu ,&nbsp;Jan Maarten van Dijl ,&nbsp;Kristina Haslinger","doi":"10.1016/j.crmicr.2025.100390","DOIUrl":"10.1016/j.crmicr.2025.100390","url":null,"abstract":"<div><div>Medicinal plants serve as valuable resources for the isolation of endophytic fungi. <em>Vinca minor</em> is a well-known producer of important vinca alkaloids and emerges as a promising source of endophytic fungi with antibacterial potential and biosynthetic capacity. In this study, we isolated an endophytic fungus from <em>V. minor</em> and identified it as <em>Cosmosporella</em> sp. VM-42<em>.</em> To date, relatively little is known about this fungal genus. The ethyl acetate extract of this isolate selectively inhibited Gram-positive bacteria, such as methicillin-sensitive and methicillin-resistant <em>Staphylococcus aureus</em> (MSSA and MRSA). Therefore, we isolated the most abundant compound from the crude extract and identified it as nectriapyrone with MIC and MBC values ranging from 125 to 62.5 µg/mL against MSSA and MRSA strains. We further sequenced and annotated the 39.07 Mb genome of the isolate, revealing that it encodes 9842 protein-coding genes, including 415 genes for carbohydrate-active enzymes and various biosynthetic gene clusters. Our untargeted metabolomic analysis shows that the fungus produces various secondary metabolites, including cyclodepsipeptides, dimeric naphtho-γ-pyrones, and macrolactones, which are known to have antifungal and antibacterial activities. In addition, we used small-molecule epigenetic modulators to activate the expression of silent biosynthetic gene clusters to broaden the chemical profile of <em>Cosmosporella</em> sp. VM-42<em>.</em> Taken together, we provide a first systematic analysis of <em>Cosmosporella</em> sp. VM-42, and our results show that it is a promising source of compounds with pharmacological potential against drug resistant bacteria.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100390"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of potentially microcystin-producing Microcystis in the Dominican Republic","authors":"Alfaniris Vargas Fernandez ,&nbsp;Patri Ramírez Ramirez ,&nbsp;Iris García Cruz ,&nbsp;Claudia Reyes Gutierrez ,&nbsp;Adrián Gutiérrez Cepeda ,&nbsp;Alexander Valdez Disla ,&nbsp;Roel Alejandro Chávez Luzania ,&nbsp;Sergio de los Santos Villalobos ,&nbsp;Pedro Leao ,&nbsp;Vitor Vasconcelos","doi":"10.1016/j.crmicr.2025.100389","DOIUrl":"10.1016/j.crmicr.2025.100389","url":null,"abstract":"<div><div>When the amount of nutrients in water bodies increases, cyanobacteria tend to proliferate rapidly in freshwater lakes and reservoirs, which can trigger cyanobacterial blooms. This increases the risk of cyanotoxin generation in water sources intended for human consumption, crop irrigation, and livestock. This study focused on identifying the presence of cyanobacteria and cyanotoxins in the Valdesia reservoir, which supplies drinking water to approximately 4 million people in Santo Domingo, Azua, San Cristóbal, San José de Ocoa, and Peravia in the Dominican Republic. Morphological observation suggested the presence of the genus <em>Microcystis</em>, which was confirmed by amplification and sequencing of two fragments of the 16S rRNA gene, as well as a fragment of the <em>mcyA</em> gene involved in encoding microcystins. This is the first report to highlight the urgent need to establish continuous monitoring of potentially microcystins-producing <em>Microcystis</em> sp. in this important reservoir, to implement appropriate water management measures to prevent their negative impact on public health and the environment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100389"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Reflexions on the role, diversity, conservation and management of the genetic microbial resources in Agriculture”","authors":"María del Pilar Rodríguez Guzmán ,&nbsp;Ismael Fernando Chávez Díaz ,&nbsp;Lily Xochilt Zelaya Molina","doi":"10.1016/j.crmicr.2025.100365","DOIUrl":"10.1016/j.crmicr.2025.100365","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100365"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental biocontamination by SARS-CoV-2 Virus in the hospital setting","authors":"M Espejo Mambié ,&nbsp;D San Jose-Saras ,&nbsp;C Bischofberger Valdés ,&nbsp;C Díaz-Agero Pérez ,&nbsp;JC Galán Montemayor ,&nbsp;L Martínez-García ,&nbsp;M Abreu Di-Berardino ,&nbsp;P Moreno-Nunez ,&nbsp;J Vicente-Guijarro ,&nbsp;J.M Aranaz-Andrés","doi":"10.1016/j.crmicr.2025.100355","DOIUrl":"10.1016/j.crmicr.2025.100355","url":null,"abstract":"<div><h3>Background</h3><div>Demonstrating the capability to isolate biological material from the environment was fundamental to supporting any transmission route. Various and inconsistent methodologies have been used to address this issue; however, the debate in scientific societies about the possibility of airborne transmission as a source of SARS-CoV-2 spread remained open.</div></div><div><h3>Objective</h3><div>To analyze SARS-CoV-2 contamination in the air and on surfaces in a hospital setting during the COVID-19 pandemic.</div></div><div><h3>Methods</h3><div>This study involved air and surface sampling in the emergency, hospitalization, and intensive care unit areas of the Ramón y Cajal University Hospital. A consistent methodology was used for all samples, and clinical and environmental parameters and characterization of each location were recorded.</div></div><div><h3>Results</h3><div>A total of 234 samples were collected, comprising 160 surface samples and 74 air samples, of which 6.84 % tested positive (13/160 surface samples and 3/74 air samples). High-contact surfaces had the highest proportion of positive samples (12/13). All positive air samples were identified within 2 m of patients who had recently developed symptoms (&lt;5 days). High dependency and elevated temperatures seemed to indicate a higher risk of environmental biocontamination. Additionally, there was a higher risk of contamination in the intensive care units than in the hospitalization or emergency units.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100355"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple influences on cyanobacterial abundance and diversity in the Beijing-Tianjin-Hebei Economic Circle and nearby areas of China","authors":"Wei Wang,&nbsp;Hanjie Huang,&nbsp;Kangxu Zhao,&nbsp;Junping Lv,&nbsp;Xudong Liu,&nbsp;Shulian Xie,&nbsp;Jia Feng","doi":"10.1016/j.crmicr.2025.100400","DOIUrl":"10.1016/j.crmicr.2025.100400","url":null,"abstract":"<div><div>In recent years, there has been a marked increase in the frequency and duration of cyanobacterial blooms, which has attracted widespread attention from all sectors of society. However, the processes and pathways leading to the formation of cyanobacterial blooms are largely stochastic, and a targeted quantitative model incorporating multiple drivers is required. A series of fieldwork and analytical procedures were conducted on 11 lakes in Beijing-Tianjin-Hebei and neighboring regions. The objective of this research was to investigate the effects of location, climate, habitat, metabolism and biology on the abundance of cyanobacteria. The findings indicated that location factors exerted an indirect influence on cyanobacterial density, with a calculated effect size of −0.001. This observation is consistent with the documented prevalence of cyanobacterial bloom outbreaks, suggesting that cyanobacterial abundance is not directly associated with location. Furthermore, the water environment (0.35 indirect and −0.37 direct effects) and climatic conditions (−0.2 indirect and 0.16 direct effects) had significant direct and indirect effects on cyanobacterial cell densities. It was also determined that high nutrients and high temperatures remained the basis for promoting cyanobacterial blooms. It is noteworthy that the microbial community exerted a substantial inhibitory effect on the proliferation of the cyanobacterial community (1.13 direct inhibitory effect). It is hypothesized that the negative effect is derived from two primary sources: the competition for ecological niches by organisms exhibiting similarity, and the direct inhibitory effect of certain bacteria on the growth of cyanobacteria, a negative correlation between acidobacteriota, bacteroide and a variety of cyanobacteria was observed in our study. The effect of specific bacteria is found to be significantly negatively correlated with the prevalence of a wide range of cyanobacteria, which is consistent with the results of many extant studies. These extant studies indicate that the formation of cyanobacterial communities is primarily influenced by microorganisms in the environment. The results of this study will provide new and complementary evidence on the mechanisms of cyanobacterial bloom occurrence and development and the design of management strategies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100400"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143928568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and discovery of an antiviral candidate inhibiting the SARS-CoV-2 envelope (2-E) channel","authors":"Han Zhang ,&nbsp;Shuxin Shi ,&nbsp;Lujia Sun ,&nbsp;Shuangqu Li ,&nbsp;Yan Zhang ,&nbsp;Ziyue Li ,&nbsp;Jingjing Hou ,&nbsp;Pingan Li ,&nbsp;Jingshan Shen ,&nbsp;Xi Cheng ,&nbsp;Shibo Jiang ,&nbsp;Zhaobing Gao ,&nbsp;Xinling Wang ,&nbsp;Xiangrui Jiang ,&nbsp;Bingqing Xia","doi":"10.1016/j.crmicr.2025.100409","DOIUrl":"10.1016/j.crmicr.2025.100409","url":null,"abstract":"<div><div>The SARS-CoV-2-encoded 2-E channel is critical in the viral life cycle and pathogenesis. By facilitating viral replication, it promotes the dysregulation of inflammatory pathways, leading to cytokine storm, and triggers DNA damage response (DDR), thus exacerbating disease progression. The 2-E channel, a viroporin, is a promising antiviral target. However, the lack of specific inhibitors and effective screening methods has hindered therapeutic exploitation of the 2-E channel. To address this gap, we report on a fluorescence-based screening assay that targets the 2-E channel activity, resulting in the identification of potential inhibitory molecules. After performing both electrophysiological studies and surface plasmon resonance (SPR) analyses, we identified the top-ranked candidate, TPN10518, as a pore-blocking inhibitor of the 2-E channel. TPN10518 binds to a hydrophobic pocket in the C-terminal vestibule of the 2-E channel, thereby inhibiting its activity. Functional evaluation showed that TPN10518 exhibits significant antiviral efficacy <em>in vitro</em>, while, at the same time, effectively protecting against 2-E channel-mediated host damage and suppressing cytokine storm caused by dysregulated homeostasis of inflammatory pathways <em>in vivo</em>. Therefore, our work introduces a screening method for targeting 2-E channels, establishes the 2-E channel as a viable therapeutic target against SARS-CoV-2, and identifies TPN10518 as a promising antiviral candidate.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100409"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}