Current Research in Microbial Sciences最新文献

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Multi-omics analysis-based insights into the microbial community composition and flavor development potentiality of different varieties of sorghum (Sorghum bicolor L. Moench) fermented into Sesame flavor Baijiu 基于多组学分析的不同品种高粱(sorghum bicolor L. Moench)发酵芝麻风味白酒的微生物群落组成及风味发展潜力研究
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-05-15 DOI: 10.1016/j.crmicr.2026.100606
Meina Jiang , Jiaxin Tang , Angui Lai , Wei Sun , Xue Liu , Zhichao Shang , Zuzhong Zheng , Xiaoxue Chen , Yansong Xue , Bei-Zhong Han
{"title":"Multi-omics analysis-based insights into the microbial community composition and flavor development potentiality of different varieties of sorghum (Sorghum bicolor L. Moench) fermented into Sesame flavor Baijiu","authors":"Meina Jiang ,&nbsp;Jiaxin Tang ,&nbsp;Angui Lai ,&nbsp;Wei Sun ,&nbsp;Xue Liu ,&nbsp;Zhichao Shang ,&nbsp;Zuzhong Zheng ,&nbsp;Xiaoxue Chen ,&nbsp;Yansong Xue ,&nbsp;Bei-Zhong Han","doi":"10.1016/j.crmicr.2026.100606","DOIUrl":"10.1016/j.crmicr.2026.100606","url":null,"abstract":"<div><div>Sorghum is a key raw ingredient in the fermentation of Sesame-flavor <em>Baijiu</em> (SFB). However, studies on the microbial and metabolic dynamics of sorghum varieties on SFB fermentation is limited. In this study, microbial and flavor differences during SFB fermentation of three sorghum varieties (<em>Hongyingzi, Hei’e</em>, and <em>Shandong</em>) were analyzed and compared. The physicochemical factors of sorghums influencing bacterial communities were greater than those affecting fungal communities. This is consistent with the finding that bacteria play a dominant role in shaping the differences in microbial community structures during fermentation with different sorghum varieties. In addition, the microbial interactions and their relationship with volatile compounds varied across different sorghum varieties. Meanwhile, the physicochemical factors that govern microbial succession also differed among these sorghum varieties. These variations collectively contribute to the observed differences in fermentation outcomes and product quality. These findings offer insights into the role of raw materials in solid-state fermentation. Specifically, they highlight how microbial dynamics and physicochemical conditions influence the consistency and quality of SFB production.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100606"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147995791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Layer-dependent modulation of S. aureus biofilms by light-induced oxidative stress 光诱导氧化应激对金黄色葡萄球菌生物膜的层依赖性调节
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-02-18 DOI: 10.1016/j.crmicr.2026.100571
Kate C. Blanco , Paul de Figueiredo , Jace A. Willis , Leonardo De Boni , Letícia P. Martinelli , Vladislav V. Yakovlev , Vanderlei S. Bagnato
{"title":"Layer-dependent modulation of S. aureus biofilms by light-induced oxidative stress","authors":"Kate C. Blanco ,&nbsp;Paul de Figueiredo ,&nbsp;Jace A. Willis ,&nbsp;Leonardo De Boni ,&nbsp;Letícia P. Martinelli ,&nbsp;Vladislav V. Yakovlev ,&nbsp;Vanderlei S. Bagnato","doi":"10.1016/j.crmicr.2026.100571","DOIUrl":"10.1016/j.crmicr.2026.100571","url":null,"abstract":"<div><h3>Background</h3><div>Bacterial biofilms display pronounced spatial heterogeneity, generating, yet how these effects are distributed across biofilm layers and how surviving cells respond after treatment remain poorly understood.</div></div><div><h3>Methods</h3><div><em>Staphylococcus aureus</em> biofilms (S biofilm) were exposed to light-induced oxidative stress, and post-treatment responses were evaluated through growth kinetics, biofilm reformation capacity, and layer-dependent photosensitizer penetration. S biofilms with different maturation times and structural complexities were analyzed to assess how biofilm architecture modulates photosensitizer distribution across superficial and deeper regions.</div></div><div><h3>Results</h3><div>Light-induced oxidative stress altered post-treatment growth dynamics and impaired the ability of surviving cells to re-establish structured biofilms. Photosensitizer penetration was strongly layer-dependent and influenced by S biofilm maturation and complexity, resulting in spatially heterogeneous photodynamic effects. These effects differentially impacted cells located in superficial versus deeper biofilm regions.</div></div><div><h3>Conclusion</h3><div>These findings demonstrate that photodynamic treatment modulates S. aureus biofilm behavior in a layer-dependent manner, weakening protective niches without requiring complete eradication. By disrupting biofilm microenvironments associated with tolerance and persistence, light-induced oxidative stress limits biofilm recovery and provides a mechanistic basis for photodynamic strategies aimed at controlling biofilm re-establishment.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100571"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147420567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phage P100 resistance in clinical and foodborne Listeria monocytogenes isolates is associated with adsorption-inhibiting mutations and fitness trade-offs 临床和食源性单核细胞增生李斯特菌分离株中噬菌体P100耐药性与吸附抑制突变和适应性权衡有关
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-02-26 DOI: 10.1016/j.crmicr.2026.100575
Christoph Brieske, Frank Hille, Erik Brinks, Hui-Zhi Low , Charles M.A.P. Franz
{"title":"Phage P100 resistance in clinical and foodborne Listeria monocytogenes isolates is associated with adsorption-inhibiting mutations and fitness trade-offs","authors":"Christoph Brieske,&nbsp;Frank Hille,&nbsp;Erik Brinks,&nbsp;Hui-Zhi Low ,&nbsp;Charles M.A.P. Franz","doi":"10.1016/j.crmicr.2026.100575","DOIUrl":"10.1016/j.crmicr.2026.100575","url":null,"abstract":"<div><div><em>Listeria monocytogenes</em> is a common bacterial pathogen causing listeriosis. Phage-based products are increasingly used in food safety, but their use raises concerns about the emergence of phage-resistant bacteria. The broad-host-range phage P100 is applied in foods, however, little is known about the genetic basis of resistance and associated fitness trade-offs in <em>L. monocytogenes</em>.</div><div>In this study, recently (2014–2020) circulating, clinical and food-associated <em>L. monocytogenes</em> strains (n = 14) were exposed to a commercial P100 phage preparation in soft agar at 20 °C and 10 °C. Eight mutants were isolated and confirmed as phage-resistant by spot assays and flow cytometry. Whole-genome sequencing revealed that most mutants derived from serovar 4b strains carried mutations in genes for polysaccharide synthesis indirectly involved in cell wall teichoic acid glycosylation, whereas mutants from serovar 2a or 2b strains had mutations in a gene directly involved in WTA glycosylation. Confocal microscopy and flow cytometry confirmed reduced phage adsorption in all mutants. The resistant mutants displayed increased antibiotic sensitivity, with two showing pronounced sensitivity to β-lactam antibiotics. These two mutants also showed impaired growth at 37 °C. Together with four additional mutants, growth was also impaired at 10 °C, both without and with sodium chloride and sodium nitrite. Overall, serovar 4b-derived mutants exhibited stronger fitness trade-offs than those of serovar 2a or 2b</div><div>The findings of this study contribute to the understanding of the P100 phage-resistance mechanisms in different serovar strains of <em>L. monocytogenes</em> and reveal associated fitness trade-offs that may be relevant for future antimicrobial and biocontrol strategies.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100575"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147420568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Omics-based dissection of alternative splicing and the regulatory architecture in fungal genomes 真菌基因组中选择性剪接和调控结构的组学分析
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-01-20 DOI: 10.1016/j.crmicr.2026.100555
João Neves-da-Rocha, Marcos E.R. Lopes, Maria J. Santos-Saboya, Mayara I.G. Azevedo, Monise F. Petrucelli, Antonio Rossi, Nilce M. Martinez-Rossi
{"title":"Omics-based dissection of alternative splicing and the regulatory architecture in fungal genomes","authors":"João Neves-da-Rocha,&nbsp;Marcos E.R. Lopes,&nbsp;Maria J. Santos-Saboya,&nbsp;Mayara I.G. Azevedo,&nbsp;Monise F. Petrucelli,&nbsp;Antonio Rossi,&nbsp;Nilce M. Martinez-Rossi","doi":"10.1016/j.crmicr.2026.100555","DOIUrl":"10.1016/j.crmicr.2026.100555","url":null,"abstract":"<div><div>Assigning mechanistic and functional associations to most of the widespread splicing diversity in fungi has been one of the continuous and most intriguing challenges in the field of molecular mycology. Ongoing advances in sequencing technologies have driven recent outbreaks in the study of the dynamic splicing landscapes of fungal transcriptomes. Nevertheless, most ascomycete and basidiomycete species of agronomic, industrial, and medical interest exhibit a notable proportion of their transcripts affected by alternative splicing (AS), producing mRNA isoforms whose regulation and functions have been largely ignored to date. In contrast to all other fungi, some yeast and basal fungal clades exhibit extremely scarce AS occurrences, which are associated with very low genomic intron counts. In this review, we provide thoughtful insights into three long-standing unsolved questions: (1) the pervasive occurrence and possible stochasticity of intron retention in fungi, (2) the reasons for increased AS rates in pathogenic and filamentous species, and (3) possible hypotheses to explain the functionality and fitness advantages of AS in fungi. We present a critical discussion on the role of AS, highlighting its importance in an evolutionary context that integrates major features of fungal diversification and adaptability. To advance our understanding of fungal splicing control, function, and evolution, we propose a practical roadmap for future research, aimed at filling the most critical gaps in the field.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100555"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146038053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The glutathione pathway is required for biofilm formation in Acinetobacter baumannii 谷胱甘肽途径是鲍曼不动杆菌形成生物膜所必需的
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-01-29 DOI: 10.1016/j.crmicr.2026.100562
Jason M. Thomas , Pegah Mosharaf Ghahfarokhy , Samantha Gabrielle Perrotti Rivera , Clarissa J. Nobile , Mamta Rawat
{"title":"The glutathione pathway is required for biofilm formation in Acinetobacter baumannii","authors":"Jason M. Thomas ,&nbsp;Pegah Mosharaf Ghahfarokhy ,&nbsp;Samantha Gabrielle Perrotti Rivera ,&nbsp;Clarissa J. Nobile ,&nbsp;Mamta Rawat","doi":"10.1016/j.crmicr.2026.100562","DOIUrl":"10.1016/j.crmicr.2026.100562","url":null,"abstract":"<div><div><em>Acinetobacter baumannii</em> is a Gram-negative nosocomial bacterium that is a member of the ESKAPE group of pathogens, notable for its virulence and intrinsic antibiotic resistance. It causes diverse infections, including respiratory and soft tissue disease, that are increasingly difficult to treat. Glutathione (GSH), the major intracellular redox buffer, is known in other bacteria to protect against stress and influence physiological processes such as biofilm formation. To investigate the roles of GSH in <em>A. baumannii</em>, we analyzed transposon mutant strains lacking <em>gshA</em> and <em>gshB</em> genes<em>,</em> which encode the enzymes that catalyze the two steps of GSH biosynthesis. Both mutant strains failed to produce GSH, exhibited impaired growth, and were hypersensitive to oxidative stress, nitrosative stress, toxins, and ferric chloride, compared to the wildtype strain. They also showed pronounced defects in biofilm formation and motility. Transcriptomic analysis of the <em>gshA</em> mutant strain relative to the wildtype strain revealed upregulation of genes involved in phenylacetate degradation and fimbrial biogenesis, while genes involved in iron and sulfur uptake and metabolism were downregulated. Moreover, mutant strains lacking GSH-dependent S-nitrosoglutathione reductases (GSNORs) displayed similar biofilm and nitrosative stress defects. Together, these findings demonstrate that GSH and GSNORs play central roles in stress resistance, biofilm development, and metabolic regulation in <em>A. baumannii</em>, highlighting their importance in the physiology and pathogenesis of this clinically relevant pathogen.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100562"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146188364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Loss of cPLA2α function attenuates inflammation and epithelial thickening in a mouse model of Haemophilus influenzae-mediated COPD exacerbation 在流感嗜血杆菌介导的慢性阻塞性肺病加重小鼠模型中,cPLA2α功能的丧失可减轻炎症和上皮增厚。
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-01-22 DOI: 10.1016/j.crmicr.2026.100556
Brice Lagrange , Fatima Benmohamed , Alice Eon-Bertho , Laurette Malleret , Alexis Trecourt , Olivier Glehen , Lhousseine Touqui , Abderrazzak Bentaher
{"title":"Loss of cPLA2α function attenuates inflammation and epithelial thickening in a mouse model of Haemophilus influenzae-mediated COPD exacerbation","authors":"Brice Lagrange ,&nbsp;Fatima Benmohamed ,&nbsp;Alice Eon-Bertho ,&nbsp;Laurette Malleret ,&nbsp;Alexis Trecourt ,&nbsp;Olivier Glehen ,&nbsp;Lhousseine Touqui ,&nbsp;Abderrazzak Bentaher","doi":"10.1016/j.crmicr.2026.100556","DOIUrl":"10.1016/j.crmicr.2026.100556","url":null,"abstract":"<div><div>Among the characteristics of chronic obstructive pulmonary diseases (COPD) especially emphysema are chronic airway inflammation and tissue destruction in response to the primary etiologic factor, cigarette smoking (CS). Importantly, COPD is aggravated by respiratory infections. Approximately half of these exacerbations are caused by bacterial infections with <em>Haemophilus influenzae</em> (<em>H. influenzae</em>) being the most common pathogen. Unfortunately, the underlying mechanism(s) of these exacerbations remain poorly understood, resulting in scarce and ineffective therapies. In this study, we investigated the relative contribution of host cytosolic phospholipase 2α (cPLA2α) to this clinical manifestation. We established a mouse model that closely mimics COPD exacerbation in which cPLA2α‐deficient mice (cPLA2α‐KO) and their wild-type littermates were exposed to CS for 4 months intercalated with periodic intranasal infections with <em>H. influenzae</em>. Our findings revealed an increased expression of a series of inflammatory mediators in the lungs of wild type, but not cPLA2α‐KO, mice. Furthermore, unlike cPLA2α‐KO lungs, wild type lungs exhibited a markedly thickenned airway epithelium. Our work shed light on a novel mechanism of COPD exacerbation that involves cPLA2α.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100556"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146167023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metagenomics reveals the functional profiles of soil microorganisms and nutrient cycling under long-term grass vegetation cropping 宏基因组学揭示了长期种植牧草土壤微生物和养分循环的功能特征
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-03-20 DOI: 10.1016/j.crmicr.2026.100583
Hengkang Xu , Jiale Guo , Chao Chen , Zhuo Pang , Guofang Zhang , Weiwei Zhang , Haiming Kan , Xinqing Shao
{"title":"Metagenomics reveals the functional profiles of soil microorganisms and nutrient cycling under long-term grass vegetation cropping","authors":"Hengkang Xu ,&nbsp;Jiale Guo ,&nbsp;Chao Chen ,&nbsp;Zhuo Pang ,&nbsp;Guofang Zhang ,&nbsp;Weiwei Zhang ,&nbsp;Haiming Kan ,&nbsp;Xinqing Shao","doi":"10.1016/j.crmicr.2026.100583","DOIUrl":"10.1016/j.crmicr.2026.100583","url":null,"abstract":"<div><div>Soil microbes are crucial for biogeochemical cycles and their functional potential is greatly affected by ecosystem management. Yet, how does grass vegetation affect the composition of soil microbial communities and the abundance of key nutrient-cycling functional genes? In this study, based on an experimental plot built for 7 years, the long – term influence of two grass vegetation types (<em>Carex breviculmis</em> and <em>Festuca arundinacea</em> Schreb) on soil microbial community structure and C, N, P, and S cycles were explored by metagenomics. The results showed that both plants significantly increased the diversity and richness of soil bacteria and fungi, and the abundance of Pseudomonadota and Ascomycota in <em>Carex breviculmis</em> increased significantly, while those of Actinomycetota and Mucoromycota decreased. Microbial network analysis shows that <em>Carex breviculmis</em> forms a highly modular, low - complexity microbial interaction network, indicating specialized and stable microbial community functions. Conversely, <em>Festuca arundinacea</em> Schreb has a more complex and less modular network, suggesting enhanced microbial interactions. <em>Carex breviculmis</em> significantly increased the abundance of genes related to carbon fixation (<em>fumA/B, pps, ppc</em>) and phosphorus mineralization (<em>phoR/P/B, phnF/P</em>), and also enhanced soil denitrification potential. In contrast, <em>Festuca arundinacea</em> Schreb showed a enrichment of soil nitrogen fixation genes (<em>nifh</em>). Additionally, growing <em>Carex breviculmis</em> and <em>Festuca arundinacea</em> Schreb induced the growth of sulfur - oxidizing bacteria (e.g., Thiobacillus), enriching the abundance of sulfur - metabolism - related genes (<em>apr, sox</em>). Genes related to microbial C, N, P, and S cycles are positively correlated with soil pH, available P, and alkali-hydrolyzed nitrogen. Overall, this study reveals how different grass vegetation types regulate microbial community structure and functional gene abundance to drive nutrient cycling differentiation in grassland ecosystems, thereby providing a theoretical basis for optimizing grass vegetation configuration in managed and restored grasslands to enhance soil ecological functions.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100583"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147702857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Targeting the US21 viroporin of human cytomegalovirus by calcium channel blockers as a new antiviral strategy 钙通道阻滞剂靶向人巨细胞病毒US21病毒蛋白的研究
IF 5.8
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-04-19 DOI: 10.1016/j.crmicr.2026.100599
Giulia Sibille , Davide Loggia , Gianluca Catucci, Alessandra Gilardino, Alessandra Fiorio Pla, Giovanna Di Nardo, Luca Munaron, Gianfranco Gilardi, Giorgio Gribaudo, Anna Luganini
{"title":"Targeting the US21 viroporin of human cytomegalovirus by calcium channel blockers as a new antiviral strategy","authors":"Giulia Sibille ,&nbsp;Davide Loggia ,&nbsp;Gianluca Catucci,&nbsp;Alessandra Gilardino,&nbsp;Alessandra Fiorio Pla,&nbsp;Giovanna Di Nardo,&nbsp;Luca Munaron,&nbsp;Gianfranco Gilardi,&nbsp;Giorgio Gribaudo,&nbsp;Anna Luganini","doi":"10.1016/j.crmicr.2026.100599","DOIUrl":"10.1016/j.crmicr.2026.100599","url":null,"abstract":"<div><div>The Human cytomegalovirus (HCMV) US21 protein is a calcium-conducting viroporin that modulates intracellular Ca<sup>2+</sup> homeostasis, safeguards cells from apoptosis, stimulates cell migration, and supports efficient HCMV replication. To validate pUS21 as a novel target for the identification of antiviral agents, <em>in silico</em> structure-based virtual screening was performed using its predicted structure to identify small molecules capable of engaging the inner part of the pore. Four dihydropyridine compounds (azelnidipine, efonidipine, lercanidipine, and niguldipine) were selected from 249 Calcium Channel Blockers (CCBs) in the DrugBank database. Molecular dynamics simulations of pUS21-ligand complexes predicted that the four selected CCBs formed dynamically stable and low-mobility interactions within the US21 pore, whereas the weak CCB binder felodipine remained highly mobile, supporting the predicted docking-based binding mode. The selected CCBs showed dose-dependent inhibition of HCMV replication in both fibroblasts and endothelial cells, with low micromolar EC<sub>50</sub> values. Their antiviral effect was neither cell type- nor strain-dependent, as confirmed against two different clinical isolates, TRwt and VR1814, and was observed to be reduced against a US21-deficient virus, suggesting the specificity of pUS21 as a molecular target. Consistent with the predicted engagement within the pUS21 pore, CCBs prevented pUS21-mediated Ca<sup>2+</sup> leakage from the endoplasmic reticulum and impaired both pUS21-induced cell migration and anti-apoptotic activity. Finally, drug combination studies revealed synergistic interactions between CCBs and maribavir treatment. Together, these findings support the hypothesis that clinically used CCBs may target pUS21 viroporin activity and hamper HCMV replication, thus offering a novel and promising antiviral strategy against HCMV, including drug-resistant strains.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100599"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147746591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sublethal oxytetracycline and copper exposure alters rRNA gene copy number, expression, and intragenomic polymorphism in ciliates 亚致死的土霉素和铜暴露改变了纤毛虫的rRNA基因拷贝数、表达和基因组内多态性
IF 5.6
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-07-10 DOI: 10.1016/j.crmicr.2026.100643
Songbao Zou, Rao Fu, Huiwen Deng, Julin Yuan, Jun Gong
{"title":"Sublethal oxytetracycline and copper exposure alters rRNA gene copy number, expression, and intragenomic polymorphism in ciliates","authors":"Songbao Zou,&nbsp;Rao Fu,&nbsp;Huiwen Deng,&nbsp;Julin Yuan,&nbsp;Jun Gong","doi":"10.1016/j.crmicr.2026.100643","DOIUrl":"10.1016/j.crmicr.2026.100643","url":null,"abstract":"<div><div>Ribosomal RNA (rRNA) genes serve as foundational markers for microbial eukaryotic diversity assessment, yet their responses to environmental stressors remain underexplored. This study examined the effects of sublethal oxytetracycline and CuCl<sub>2</sub> on phenotypic and ribotypic traits in the ciliated protists <em>Paramecium bursaria</em> and <em>Euplotes vannus</em> using single-cell quantitative PCR and high-throughput sequencing of 18S rDNA/rRNA. We found that both pollutants inhibited growth, enlarged cell volume, and elevated per-cell rDNA and rRNA copy numbers, but oxytetracycline selectively elevated rRNA: rDNA ratios. These effects increased the variance around established allometric scaling relationships between rDNA copy number and cell volume, without significantly altering the scaling slope. Intragenomic polymorphisms also increased, with the amplicon sequence variant (ASV) number of rRNA transcripts exceeding that of rDNA, and these elevated polymorphisms persisted post-stress relief. Re-analysis of field 18S metabarcoding data from CuCl<sub>2</sub>-polluted marine biofilms revealed dose-dependent increases in the number of ASVs per operational taxonomic unit (OTU, defined at a cutoff of 97% sequence identity) in many protistan groups, suggesting that copper-induced mutagenic pressure on ribosomal RNA genes may prevail across diverse protistan taxa. Our findings imply that: (1) when interpreting ASV-based microbial diversity patterns, the potential for ASV richness inflation and elevated per-cell rDNA and rRNA abundances should be recognized as a likely consequence of exposure to environmental pollutants; (2) there is a potential for using the ASV-to-OTU number ratio in assessing environmental stress; and (3) metabarcoding of rRNA likely introduces more artificial ASVs than targeting rDNA. A combination of double metabarcoding of both rDNA and rRNA to identify active microbial members is recommended.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100643"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148568051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Erratum to ‛Mangrove cyanobacterial diversity as a source of bioactive natural products’ [Current Research in Microbial Sciences 10 (2026) 100557] “红树林蓝藻多样性作为生物活性天然产物的来源”的勘误[微生物科学研究现状10 (2026)100557]
IF 5.6
Current Research in Microbial Sciences Pub Date : 2026-01-01 Epub Date: 2026-06-12 DOI: 10.1016/j.crmicr.2026.100619
Arup Ratan Roy, Shayontani Basu, Sergio de los Santos-Villalobos, Joydeep Mukherjee
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