Current Research in Microbial Sciences最新文献

{"title":"Erratum to ‘Draft genome sequence of Bacillus sp. strain FSQ1, a biological control agent against white mold in common bean (Phaseolus vulgaris L.)’ [Current Research in Microbial Sciences Volume 3, 2022, 100138]","authors":"Carmen María Félix-Pablos, Fannie I. Parra-Cota, Gustavo Santoyo, Ma. del Carmen Orozco-Mosqueda, Sergio de los Santos-Villalobos","doi":"10.1016/j.crmicr.2026.100628","DOIUrl":"10.1016/j.crmicr.2026.100628","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100628"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148730007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, identification, and plant growth-promoting mechanisms of strain BN5, with a focus on exogenously Trp-independent IAA biosynthesis, and its impact on cucumber cultivation","authors":"Qiannan Cui ,&nbsp;Jie Wang ,&nbsp;Yujie Huang ,&nbsp;Fangyuan Zhou ,&nbsp;Jinjuan Wu ,&nbsp;Fanyong Song ,&nbsp;Qingqing Zhao ,&nbsp;Rulong Feng ,&nbsp;Xinjian Zhang","doi":"10.1016/j.crmicr.2026.100549","DOIUrl":"10.1016/j.crmicr.2026.100549","url":null,"abstract":"<div><div>The genus <em>Niallia</em> was recently separated as a taxonomic group from <em>Bacillus</em> based on conserved signature insertions and deletions in the genome. Unlike its role in bioremediation, its function in plant growth promotion has not attracted widespread attention. This study identifies <em>Niallia taxi</em> BN5, focusing on the role of tryptophan in IAA synthesis and regulation, as well as the effects of cucumber. The study employed pot and greenhouse experiments to evaluate the impacts of BN5 on cucumber growth, yield, and quality. Genome analysis explored the tryptophan-regulated IAA biosynthesis pathway, with transcriptome and qPCR examining expression of IAA-related, tryptophan synthesis, and motility genes. It also evaluated impacts on rhizosphere microbial communities and soil enzyme activities. BN5 significantly enhanced cucumber growth (height: +24.29 %, stem diameter: +11.23 %), yield (+17.55 %), and quality (vitamin C: +17.2 % soluble sugar: +37.41 %, all <em>p</em> <em>&lt;</em> <em>0.05</em>). It increased soil enzyme activities (urease by +52.12 %, dehydrogenase by +44.70 %, <em>p</em> <em>&lt;</em> <em>0.05</em>) and reshaped the microbial structure. Owing to its possession of a complete tryptophan synthase operon (<em>trp</em>E-D-G-C-F-B-A), strain BN5 is able to produce IAA at a concentration of 17.64 μg/mL via a tryptophan-dependent biosynthetic pathway, with no reliance on exogenous tryptophan. Exogenous tryptophan downregulated the <em>trp</em> cluster but upregulated motility genes (<em>mcp</em> and <em>fli</em>C, <em>p</em> <em>&lt;</em> <em>0.05</em>), indicating adaptive regulation of IAA precursors. This study clarifies the plant growth-promoting mechanisms of <em>Niallia</em> spp., focusing on tryptophan-mediated regulation of IAA biosynthesis that is independent of exogenous tryptophan. It lays a foundation for efficient microbial fertilizers, highlighting BN5′s potential in sustainable agriculture.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100549"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145925395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed bacterial microbiota: transmission, community assembly, and prospects for engineering heritable functions in crops","authors":"Hamed Azarbad,&nbsp;Mehrdad Alizadeh","doi":"10.1016/j.crmicr.2026.100647","DOIUrl":"10.1016/j.crmicr.2026.100647","url":null,"abstract":"<div><div>Seeds represent specialized, low-biomass microbial niches that facilitate the transmission of the plant-associated microbiota from one generation to another. Residing as both epiphytes and endophytes, seed-associated bacteria contribute to microbial community assembly during the seed-to-seedling transition. Recent integration of single-seed omics and synthetic bacterial communities has moved the field beyond descriptive surveys to identify the microbial drivers of plant germination success and early-life resilience. Despite this potential, the establishment and persistence of seed-borne bacteria are often constrained by competition with soil-derived microbiota. Seedling bacterial community assembly is further shaped by host genotype, domestication history, and maternal environmental conditions. In this review, we synthesize the mechanisms of bacterial transmission from flowering through seedling emergence and evaluate the functional capacity of the seed holobiont. We define the ecological parameters for the successful recruitment of engineered seed-based consortia into the soil-plant continuum, providing an experimental roadmap to move seed-based technologies from the laboratory to the field. Ensuring the functional stability of these heritable microbial traits will be essential for stabilizing agricultural productivity under increasing climatic volatility.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100647"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148640985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qdr3 Coordinates cellular homeostasis, mitochondrial remodeling, and virulence in Candidozyma auris (Candida auris)","authors":"Isadora F Munhoz da Rocha,&nbsp;Craig L. Ennis,&nbsp;Bruno AA Romagnoli,&nbsp;Kayo Schemiko,&nbsp;Suelen A Rossi,&nbsp;Alexandre T da Costa,&nbsp;Haroldo C de Oliveira,&nbsp;Clarissa J Nobile,&nbsp;Lysangela R Alves","doi":"10.1016/j.crmicr.2026.100645","DOIUrl":"10.1016/j.crmicr.2026.100645","url":null,"abstract":"<div><div>Qdr3 acts as a global regulator in <em>Candidozyma auris</em> (<em>Candida auris</em>), coordinating mitochondrial function and cell-surface architecture. Loss of <em>qdr3</em> causes major cellular reprogramming, increasing mitochondrial activity and virulence, highlighting its key role in fungal homeostasis and pathogenicity. The graphical abstract was generated by the Notebook LM tool by Google using the following prompt: “Create a visual abstract for scientific journal submission (BMJ standard). Ensure: (1) accurate spelling, and (2) no fabrication—use only data from the manuscript. Ensure the image is 531×1328 pixels (h x w) or proportionally more, and is readable at a size of 5 × 13 cm.”<span><figure><span><img><ol><li><span><span>Download: <span>Download high-res image (310KB)</span></span></span></li><li><span><span>Download: <span>Download full-size image</span></span></span></li></ol></span></figure></span></div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100645"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148642127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lineage-specific endosymbiosis in Mucorales: restriction of Mycetohabitans to the genus Rhizopus","authors":"Na Li ,&nbsp;Jochem Buil ,&nbsp;Qi-Rui Li ,&nbsp;Anuradha Chowdhary ,&nbsp;Shao-Qin Zhou ,&nbsp;Ying-Qian Kang ,&nbsp;Sybren de Hoog","doi":"10.1016/j.crmicr.2026.100595","DOIUrl":"10.1016/j.crmicr.2026.100595","url":null,"abstract":"<div><div>Endosymbiotic bacteria have been reported in mucoralean fungi, yet their taxonomic distribution, range of ecological niches, and host specificity remain incompletely understood. Clarifying the occurrence of these bacterial partners across clinical and environmental Mucorales is essential for understanding their evolutionary and biological significance. In this study, we screened 578 isolates of Mucorales from both clinical and fermented food sources, including 360 from mucormycosis patients in The Netherlands, 40 from COVID-19-associated mucormycosis (CAM) patients in India, and 178 foodborne isolates from fermented soybean foods in China. Although 16S rRNA gene amplification revealed the presence of bacteria in sixteen mucoralean isolates, fluorescence in situ hybridization (FISH) demonstrated intracellular localization in only five of them. In all five cases, the endobacteria were identified as <em>Mycetohabitans</em>, and all corresponding fungal hosts belonged to <em>Rhizopus</em> species, suggesting that bacterial endosymbiosis within Mucorales is primarily restricted to this genus. Notably, a <em>Rhizopus homothallicus</em> isolate was found to harbor <em>Mycetohabitans</em> sp., with 98.37% 16S rRNA gene sequence similarity to the type of <em>M. rhizoxinica</em>, forming a separate phylogenetic clade and potentially representing a novel lineage. Endosymbionts were not detected in foodborne Mucorales from China, consistent with the predominance of <em>Mucor</em> species in these samples. Together, these results demonstrate a lineage-specific association between <em>Mycetohabitans</em> and <em>Rhizopus</em> species and highlight a lineage-dependent pattern across ecological niches. This study provides a systematic approach to evaluating fungal-bacterial symbiosis and offers a basis for future investigations into the functional and ecological roles of endosymbiotic bacteria in Mucorales.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100595"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147746588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and structural analysis of major antigenic proteins' variability in the human relapsing fever spirochete Borrelia persica","authors":"Dor Shwartz ,&nbsp;Yaarit Nachum-Biala ,&nbsp;Reinhard K. Straubinger ,&nbsp;Ofir Segev ,&nbsp;Asaf Levy ,&nbsp;Job E. Lopez ,&nbsp;Gad Baneth","doi":"10.1016/j.crmicr.2026.100598","DOIUrl":"10.1016/j.crmicr.2026.100598","url":null,"abstract":"<div><div>Antigenic variation enables immune evasion of pathogens, yet the mechanisms for the rapid change in their antigens without loss of structural and functional features are not fully known. Tick-borne relapsing fever (TBRF) spirochetes of the genus <em>Borrelia</em> can be used as a model because their variable major proteins (Vmps) drive antigenic variation followed by recurrent waves of bacteremia. In this study we generated the genome of <em>Borrelia persica</em> LMU-C01 and evaluated Vmp structure across relapsing fever <em>Borrelia</em> species. <em>Borrelia persica</em> was found to have a genome of 1.7Mb with one linear chromosome, 12 linear and one circular plasmids. Telomeric hairpin motifs characteristic of <em>Borrelia</em> linear replicons were detected in the chromosome and in the linear plasmids. Pulsed-field gel electrophoresis indicated that plasmid profiles remained identical after a year of continuous in-vitro cultivation. Genome annotation revealed 48 complete variable large proteins (Vlps) and one variable small protein (Vsp), all encoded in plasmids. Comparative structure modeling of <em>B. persica</em> Vlps together with 420 Vlps from twelve TBRF species revealed a conserved, surface-exposed variable region that occupies a similar external predicted position across structures, even as primary sequences diverge. This indicated that antigenic variation preserves topology while tolerating sequence plasticity, which may be shared with other immune evasive bacteria.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100598"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147746590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress-induced remodeling of membrane-bound organelles in budding yeast","authors":"Peng Sheng,&nbsp;Mengna Sun,&nbsp;Xinyue Wang,&nbsp;Lizhe Bai,&nbsp;Hong Cao,&nbsp;Dan Li","doi":"10.1016/j.crmicr.2026.100653","DOIUrl":"10.1016/j.crmicr.2026.100653","url":null,"abstract":"<div><div>Membrane-bound organelles undergo extensive remodeling during environmental stress, yet systematic side-by-side comparisons of organelle responses in budding yeast remain limited. Here, a panel of fluorescent markers was used to examine multiple organelles in <em>Saccharomyces cerevisiae</em> exposed to heat, hydrogen peroxide, acetic acid, or ethanol. Across all conditions, mitochondria consistently shifted from tubular networks to fragmented puncta, representing a common stress response. Quantitative scoring showed that heat stress induced mitochondrial fragmentation in more than 90% of cells within 20 min, and GFP-HDEL redistribution was detected in a substantial fraction of cells under all four stress conditions, with the strongest effect under heat stress. In contrast, overall endoplasmic reticulum (ER) morphology remained largely preserved, although redistribution of GFP-HDEL indicated altered ER retention and/or endomembrane homeostasis. Heat and oxidative stress also induced Ire1 puncta. Several nuclear proteins exhibited stress-dependent redistribution from the nucleus, indicating dynamic remodeling of nuclear protein localization. Vacuoles generally appeared enlarged and fused, whereas acetic acid induced a distinct phenotype with Ybh3 enrichment at the vacuolar membrane and redistribution of Prc1 and Pep4 to cytoplasmic puncta. Markers of the early and late Golgi and the late endosome showed stress-specific loss, clustering, or relocalization. Lipid droplets, peroxisomes, and autophagy-related structures were also altered. Additionally, Yca1, Aif1, and Mmi1 formed puncta under heat and ethanol stress. Together, these findings provide a comparative imaging framework defining shared and stress-specific features of organelle remodeling in budding yeast.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100653"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148730005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum to ‘Advances in microbial based bio-inoculum for amelioration of soil health and sustainable crop production’ [Current Research in Microbial Sciences Volume 7, 2024, 100251]","authors":"Aurodeepa Samantaray,&nbsp;Sourav Chattaraj,&nbsp;Debasis Mitra,&nbsp;Arindam Ganguly,&nbsp;Rahul Kumar,&nbsp;Ashish Gaur,&nbsp;Pradeep K.Das Mohapatra,&nbsp;Sergio de los Santos-Villalobos,&nbsp;Anju Rani,&nbsp;Hrudayanath Thatoi","doi":"10.1016/j.crmicr.2026.100623","DOIUrl":"10.1016/j.crmicr.2026.100623","url":null,"abstract":"","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100623"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148730653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antiviral potential of black tea and blueberry extracts against monkeypox virus by targeting orthopoxvirus surface proteins","authors":"Yi’ou Sha ,&nbsp;Yixin Li ,&nbsp;Yanchen Liu ,&nbsp;Jiannan Chen ,&nbsp;Chen Hua ,&nbsp;Yangguang Fu ,&nbsp;Xinxin Yuan ,&nbsp;Yan Yan ,&nbsp;Suya Jin ,&nbsp;Yitong Li ,&nbsp;Anqi Xia ,&nbsp;Qian Wang ,&nbsp;Qiao Wang ,&nbsp;Yinghua Chen ,&nbsp;Rong Zhang ,&nbsp;Shibo Jiang ,&nbsp;Jian Ying","doi":"10.1016/j.crmicr.2026.100608","DOIUrl":"10.1016/j.crmicr.2026.100608","url":null,"abstract":"<div><div>Monkeypox virus (MPXV) outbreaks primarily affect resource-limited regions in Africa, highlighting an urgent need for low-cost, accessible therapeutics. Food-medicine homologous (FMH) substances, which are bioactive food components with medicinal properties, offer a promising therapeutic avenue owing to their low cost, favorable safety, and oral bioavailability. Here, we report that black tea extract (BTE) and blueberry extract (BBE), two representative FMH substances, can inhibit the vaccinia virus (VACV) and MPXV. Mechanistic studies suggest that BTE and BBE act at the post-attachment stage of viral entry by interacting with the orthopoxvirus surface proteins B6R and L1R. In a murine model of VACV infection, both extracts demonstrated modest <em>in vivo</em> antiviral efficacy. Furthermore, BTE and BBE exhibit <em>in vitro</em> synergistic antiviral activity when combined with each other or 3-hydroxypropionic acid-modified β-lactoglobulin (3HP-β-LG), a reported antiviral protein. These findings position BTE and BBE as promising, cost-effective candidates for further development as antivirals against MPXV in African regions.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"10 ","pages":"Article 100608"},"PeriodicalIF":5.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148158300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial drivers of soil health: Integrating physical, chemical and biological properties for food security under climate change","authors":"Gloria Elva Dorado-González,&nbsp;Alberto Muro-Reyes,&nbsp;Sergio de los Santos-Villalobos,&nbsp;Daniel García-Cervantes,&nbsp;Jesús Gerardo García-Olivares,&nbsp;Héctor Manuel Robles-Berlanga,&nbsp;Héctor Gutiérrez-Bañuelos","doi":"10.1016/j.crmicr.2026.100636","DOIUrl":"10.1016/j.crmicr.2026.100636","url":null,"abstract":"<div><div>Climate change is intensifying heat, drought/flooding extremes, salinity, and CO₂-driven shifts that disrupt soil structure, chemistry, and biological activity, with cascading consequences for crop productivity and food security. This review synthesizes evidence that soil health results from the interconnected interactions among physical structure (aggregation, porosity, bulk density, and pore connectivity), chemical constraints (pH, salinity, nutrient availability, cation exchange capacity, and redox heterogeneity), and biological activity (microbial biomass, diversity, and functional pathways). A central conclusion is that climate impacts are frequently mediated through pore-scale microhabitats (oxygen and moisture gradients, redox microsites, and substrate accessibility), which reorganize microbial functional guilds and regulate C-N-P transformations, organic matter turnover, and aggregation dynamics. We highlight mechanistic pathways by which microbiomes actively shape soil resilience, including EPS/biofilm-mediated aggregate stabilization, extracellular enzyme systems that control depolymerization and nutrient acquisition, and metabolite-driven nutrient mobilization (e.g., organic acids and siderophores), alongside nitrogen and phosphorus cycling processes that are highly sensitive to aeration and moisture regimes. Evidence across agroecosystems indicates that effective climate-smart soil management is most robust when “habitat-first” practices (reduced disturbance, continuous plant inputs, organic amendments) are combined with context-dependent microbiome steering (diversified rotations/cover crops and targeted inoculants). Overall, integrating cross-domain indicators with mechanistic understanding offers actionable pathways to strengthen soil multifunctionality, stabilize yields under climate variability, and support sustainable food systems</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"11 ","pages":"Article 100636"},"PeriodicalIF":5.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13330518/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148391512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}