Current Research in Microbial Sciences最新文献

{"title":"The multidrug-resistant Candida auris, Candida haemulonii complex and phylogenetic related species: Insights into antifungal resistance mechanisms","authors":"Lívia S. Ramos ,&nbsp;Pedro F. Barbosa ,&nbsp;Carolline M.A. Lorentino ,&nbsp;Joice C. Lima ,&nbsp;Antonio L. Braga ,&nbsp;Raquel V. Lima ,&nbsp;Lucas Giovanini ,&nbsp;Ana Lúcia Casemiro ,&nbsp;Nahyara L.M. Siqueira ,&nbsp;Stefanie C. Costa ,&nbsp;Célia F. Rodrigues ,&nbsp;Maryam Roudbary ,&nbsp;Marta H. Branquinha ,&nbsp;André L.S. Santos","doi":"10.1016/j.crmicr.2025.100354","DOIUrl":"10.1016/j.crmicr.2025.100354","url":null,"abstract":"<div><div>The rise of multidrug-resistant (MDR) fungal pathogens poses a serious global threat to human health. Of particular concern are <em>Candida auris</em>, the <em>Candida haemulonii</em> complex (which includes C<em>. haemulonii sensu stricto, C. duobushaemulonii</em> and <em>C. haemulonii</em> var. <em>vulnera</em>), and phylogenetically related species, including <em>C. pseudohaemulonii</em> and <em>C. vulturna</em>. These emerging, widespread, and opportunistic pathogens have drawn significant attention due to their reduced susceptibility to commonly used antifungal agents, particularly azoles and polyenes, and, in some cases, therapy-induced resistance to echinocandins. Notably, <em>C. auris</em> is classified in the critical priority group on the World Health Organization's fungal priority pathogens list, which highlights fungal species capable of causing systemic infections with significant mortality and morbidity risks as well as the challenges posed by their MDR profiles, limited treatment and management options. The mechanisms underlying antifungal resistance within these emerging fungal species is still being explored, but some advances have been achieved in the past few years. In this review, we compile current literature on the distribution of susceptible and resistant clinical strains of <em>C. auris, C. haemulonii</em> complex, <em>C. pseudohaemulonii</em> and <em>C. vulturna</em> across various antifungal classes, including azoles (fluconazole, voriconazole, itraconazole), polyenes (amphotericin B), echinocandins (caspofungin, micafungin, anidulafungin), and pyrimidine analogues (flucytosine). We also outline the main antifungal resistance mechanisms identified in planktonic cells of these yeast species. Finally, we explore the impact of biofilm formation, a classical virulence attribute of fungi, on antifungal resistance, highlighting the resistance mechanisms associated with this complex microbial structure that have been uncovered to date.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100354"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143241127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evolution of antifungal therapy: Traditional agents, current challenges and future perspectives","authors":"Cássia Milena de Souza ,&nbsp;Bárbara Tavares Bezerra ,&nbsp;Daniel Agreda Mellon ,&nbsp;Haroldo Cesar de Oliveira","doi":"10.1016/j.crmicr.2025.100341","DOIUrl":"10.1016/j.crmicr.2025.100341","url":null,"abstract":"<div><div>Fungal infections kill more than 3 million people every year. This high number reflects the significant challenges that treating these diseases worldwide presents. The current arsenal of antifungal drugs is limited and often accompanied by high toxicity to patients, elevated treatment costs, increased frequency of resistance rates, and the emergence of naturally resistant species. These treatment challenges highlight the urgency of developing new antifungal therapies, which could positively impact millions of lives each year globally. Our review offers an overview of the antifungal drugs currently available for treatment, presents the status of new antifungal drugs under clinical study, and explores ahead to future candidates that aim to help address this important global health issue.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100341"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11786858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cooperative mechanisms of LexA and HtpG in the regulation of virulence gene expression in Pseudomonas plecoglossicida","authors":"Rongchao He ,&nbsp;Yanfei Zuo ,&nbsp;Qiu Li ,&nbsp;Qingpi Yan ,&nbsp;Lixing Huang","doi":"10.1016/j.crmicr.2025.100351","DOIUrl":"10.1016/j.crmicr.2025.100351","url":null,"abstract":"<div><div>LexA is a well-known transcriptional repressor of DNA repair genes induced by DNA damage in <em>Escherichia coli</em> and other bacterial species. Recently, this paradigm—that LexA solely regulates the SOS response—has been challenged as studies reveal its involvement in various biological functions linked to virulence. <em>Pseudomonas plecoglossicida</em>, a major pathogen in mariculture, causes substantial economic losses annually in China. Our previous research suggested that LexA might collaboratively regulate virulence gene expression with HtpG during infection. This study aims to elucidate the molecular mechanism by which LexA controls virulence gene expression. We employed an array of methods including molecular dynamics simulations, molecular docking, ChIP-seq, RNA-seq, mass spectrometry, gene mutagenesis, LacZ reporter assays, electrophoretic mobility shift assays, co-immunoprecipitation, and in vitro LexA degradation experiments. Our findings identified 36 downstream virulence genes regulated by LexA, define three critical LexA binding motifs, and provide an in-depth analysis of LexA's recognition and binding to promoters, thereby regulating virulence gene expression. Additionally, we confirm the cooperative regulatory roles of HtpG, RecA, and LexA in virulence gene modulation. This is the first report of an endogenous accessory factor aiding in the binding of LexA to DNA. This study enhances our understanding of LexA's role in virulence regulation and offers a valuable theoretical and practical foundation for disease prevention and control.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100351"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of synergistic fermentation of tea bee pollen with bacteria and enzymes on growth and intestinal health of Apis cerana cerana","authors":"Panpan Xue ,&nbsp;Le Xu ,&nbsp;Yakai Tian ,&nbsp;Mingkui Lv ,&nbsp;Pingping Fang ,&nbsp;Kun Dong ,&nbsp;Qiuye Lin ,&nbsp;Zhenhui Cao","doi":"10.1016/j.crmicr.2025.100343","DOIUrl":"10.1016/j.crmicr.2025.100343","url":null,"abstract":"<div><div>While the health benefits of lactic acid bacteria (LAB)-fermented feed on farmed animals are well-established, its potential benefits for honeybees, specifically <em>Apis cerana cerana</em>, remain largely unexplored. The present study aimed to optimize an enzymatic hydrolysis process for tea bee pollen, employing a complex enzyme comprising acid cellulase and pectinase, followed by fermentation with <em>Limosilactobacillus reuteri</em> LP4. <em>A. c. cerana</em> workers were subsequently fed tea bee pollen processed with this optimized method. Under the optimal processing condition of fermented tea bee pollen, the pH value was 4.41, the protein content was 27.75 %, and the viable count of LAB was 2.31×10⁹ CFU/g. No molds and yeasts as well as pathogens were detected. Compared to the unfermented pollen, honey bee workers administrated with fermented tea pollen with L. <em>reuteri</em> LP4 showed significantly increased survival rate by 24.34 % on day 15. Moreover, the relative abundances of <em>Lactobacillus</em> and <em>Bifidobacterium</em> were elevated, while those of <em>Enterococcus</em> and <em>Bacteroides</em> were diminished. Concurrently, the relative expression levels of immune-related genes including <em>Abaecin, PPO, Defensin</em>, and <em>Vg</em> were significantly upregulated. These findings provide a scientific foundation for application of fermented feeds to enhance the health of <em>A. c. cerana</em> populations and contribute to the sustainable development of apiculture in China.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100343"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel mode of histone-like protein HupB regulating Sinorhizobium meliloti cell division through lysine acetylation","authors":"Ningning Li ,&nbsp;Huibo Jin ,&nbsp;Hongbo Li ,&nbsp;Huilin Yu ,&nbsp;Xiaoxu Wu ,&nbsp;Tianci Zhang ,&nbsp;Liangliang Yu ,&nbsp;Zhaoling Qin ,&nbsp;Li Luo","doi":"10.1016/j.crmicr.2025.100345","DOIUrl":"10.1016/j.crmicr.2025.100345","url":null,"abstract":"<div><div>HU, a small, basic histone-like protein, binds to bacterial genomic DNA, influencing DNA conformation, replication, and transcription. Its acetylation is a key post-translational modification affecting its DNA-binding activity. The role of HU acetylation in regulating cell division through the cell cycle regulatory system remained largely unknown. In this study, we find that stimulation of lysine acetylation or non-acetylation in HupB, a homolog of HU, differentially regulates the expression of cell cycle regulators, as well as cell growth and division in <em>Sinorhizobium meliloti</em>. Lys3, Lys13, and Lys83 in HupB were identified as acetylated residues by mass spectrometry. Mutating these residues to arginine (stimulating non-acetylation) in HupB impedes normal cell division, while substituting them with glycine (mimicking acetylation) allows for rapid cell duplication. The mimicry of non-acetylated HupB leads to enlarged abnormal cells, while stimulating acetylated HupB only reduces cell length. Transcription activation was observed in the mutant cells. Cell cycle regulators such as CtrA, GcrA and DnaA were differentially expressed in the mutants. HupB substitutions differentially bound to these cell cycle regulatory genes. These findings suggest that the appropriate acetylation of HupB regulates the expression of cell cycle regulators, thereby controlling <em>S. meliloti</em> cell division.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100345"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143105138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of amphipathic antimicrobial peptides with broad spectrum activity against multi-drug resistant bacteria.","authors":"Mireia López-Siles ,&nbsp;Ana Tajuelo ,&nbsp;Pau Caravaca-Fuentes ,&nbsp;Marta Planas ,&nbsp;Lidia Feliu ,&nbsp;Michael J. McConnell","doi":"10.1016/j.crmicr.2025.100363","DOIUrl":"10.1016/j.crmicr.2025.100363","url":null,"abstract":"<div><div>Antimicrobial peptides (AMPs) are potential alternatives to antibiotics given the reduced likelihood of resistance and their high selectivity towards bacteria. AMPs with activity against antibiotic-resistant bacteria have been reported. The aim of this study is to characterize the activity of novel <strong>BP100</strong> analogues against multidrug-resistant bacteria. Eleven bacterial strains representing five pathogenic species were used to evaluate the antimicrobial activity of 26 peptides. An initial screen was performed at 50 µg/ml, and those peptides that inhibited ≈90 % of growth of all strains were selected. Minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), inhibition in biofilm formation, time kill assays, stability in human serum and <em>in vivo</em> toxicity were assessed. <strong>BP607, BP76</strong> and <strong>BP145,</strong> had broad activity against multidrug-resistant bacteria. MICs ranged between 3.13 and 50 µg/ml, whereas MBCs ranged between 6.25 and 100 µg/ml. <em>Acinetobacter baumannii, Klebsiella pneumoniae</em> and <em>Escherichia coli</em> were the most susceptible species. At 2x the MIC, all compounds were bactericidal after 6h. <strong>BP76</strong> inhibited ≥ 76.77 % of <em>K. pneumoniae</em> and <em>E. coli</em> biofilm formation at subinhibitory concentrations. <strong>BP145</strong> had improved serum stability and lower toxicity compared to <strong>BP607</strong>. In conclusion, <strong>BP145</strong> and <strong>BP76</strong> demonstrate broad antimicrobial activity, are active at non-toxic concentrations, feature bactericidal activity at 6h and inhibit biofilm formation.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100363"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pyricularia pennisetigena and Pyricularia oryzae isolates from Paraguay's wheat-growing regions and the impact on wheat","authors":"Cinthia Carolina Cazal-Martínez ,&nbsp;Yessica Magaliz Reyes-Caballero ,&nbsp;Alice Rocio Chávez ,&nbsp;Pastor Enmanuel Pérez-Estigarribia ,&nbsp;Man Mohan Kohli ,&nbsp;Alcides Rojas ,&nbsp;Andrea Alejandra Arrua ,&nbsp;Juliana Moura-Mendes ,&nbsp;Ramón Souza-Perera ,&nbsp;José Juan Zúñiga Agilar ,&nbsp;Emile Gluck-Thaler ,&nbsp;Horacio Lopez-Nicora ,&nbsp;Julio Cesar Masaru Iehisa","doi":"10.1016/j.crmicr.2025.100361","DOIUrl":"10.1016/j.crmicr.2025.100361","url":null,"abstract":"<div><div>The <em>Pyricularia</em> genus includes species causing blast disease in monocots, posing significant challenges for disease management due to their ability to infect multiple hosts. This study aimed to identify the pathogenicity and species identity of <em>Pyricularia</em> isolates from 11 plant species in wheat-growing regions of Paraguay and assess their capacity to infect wheat. Twenty-four monosporic isolates were analyzed based on macroscopic and microscopic and phylogenetic characteristics. Three phylogenetic clades corresponding to <em>P. oryzae, P. grisea</em>, and <em>P. pennisetigena</em> were identified through five barcoding genes. For the first time, wheat blast was reported in San Pedro Department, and blast disease was observed in weeds in Cordillera and Central Departments. In greenhouse trials, <em>P. oryzae</em> isolates from wheat successfully infected both susceptible and resistant wheat cultivars, whereas isolates from non-wheat hosts did not elicit symptoms. Notably, <em>P. pennisetigena</em> isolates derived from <em>Cenchrus echinatus</em> were capable of infecting wheat spikes, producing typical blast symptoms, highlighting the potential for cross-species pathogen transmission. This finding suggests <em>P. pennisetigena</em> may pose an emerging threat to wheat in Paraguay, as its primary host is prevalent near wheat fields. These results highlight the critical importance of integrated disease management strategies, particularly the identification of inoculum sources, to mitigate cross-species pathogen transmission. This approach aligns with the One Health paradigm by addressing interconnected risks to plant health, food security, and environmental sustainability.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100361"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143512053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-omics unveils strain-specific neuroactive metabolite production linked to inflammation modulation by Bacteroides and their extracellular vesicles","authors":"Basit Yousuf ,&nbsp;Walid Mottawea ,&nbsp;Galal Ali Esmail ,&nbsp;Nazila Nazemof ,&nbsp;Nour Elhouda Bouhlel ,&nbsp;Emmanuel Njoku ,&nbsp;Yingxi Li ,&nbsp;Xu Zhang ,&nbsp;Zoran Minic ,&nbsp;Riadh Hammami","doi":"10.1016/j.crmicr.2025.100358","DOIUrl":"10.1016/j.crmicr.2025.100358","url":null,"abstract":"<div><div><em>Bacteroides</em> species are key members of the human gut microbiome and play crucial roles in gut ecology, metabolism, and host-microbe interactions. This study investigated the strain-specific production of neuroactive metabolites by 18 Bacteroidetes (12 <em>Bacteroides</em>, 4 <em>Phocaeicola</em>, and 2 <em>Parabacteroides</em>) using multi-omics approaches. Genomic analysis revealed a significant potential for producing GABA, tryptophan, tyrosine, and histidine metabolism-linked neuroactive compounds. Using untargeted and targeted metabolomics, we identified key neurotransmitter-related or precursor metabolites, including GABA, <span>l</span>-tryptophan, 5-HTP, normelatonin, kynurenic acid, <span>l</span>-tyrosine, and norepinephrine, in a strain- and media-specific manner, with GABA (1–2 mM) being the most abundant. Additionally, extracellular vesicles (EVs) produced by <em>Bacteroides</em> harbor multiple neuroactive metabolites, mainly GABA, and related key enzymes. We used CRISPR/Cas12a-based gene engineering to create a knockout mutant lacking the glutamate decarboxylase gene (<em>gadB</em>) to demonstrate the specific contribution of <em>Bacteroides finegoldii</em>-derived GABA in modulating intestinal homeostasis. Cell-free supernatants from wild-type (WT, GABA+) and Δ<em>gadB</em> (GABA-) provided GABA-independent reinforcement of epithelial membrane integrity in LPS-treated Caco-2/HT29-MTX co-cultures. EVs from WT and Δ<em>gadB</em> attenuated inflammatory immune response of LPS-treated RAW264.7 macrophages, with reduced pro-inflammatory cytokines (IL-1β and IL-6), downregulation of TNF-α, and upregulation of IL-10 and TGF-β. GABA production by <em>B. finegoldii</em> had a limited impact on gut barrier integrity but a significant role in modulating inflammation. This study is the first to demonstrate the presence of a myriad of neuroactive metabolites produced by <em>Bacteroides</em> species in a strain- and media-specific manner in supernatant and EVs, with GABA being the most dominant metabolite and influencing immune responses.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100358"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and identification of metabolites produced by Cytobacillus firmus in the interaction with Arabidopsis thaliana plants and their effect on Solanum lycopersicum","authors":"Itzel G. Arteaga-Ríos ,&nbsp;Karen Beatriz Méndez-Rodríguez ,&nbsp;Raul Ocampo-Pérez ,&nbsp;María de la Luz Guerrero-González ,&nbsp;Raúl Rodríguez-Guerra ,&nbsp;Pablo Delgado-Sánchez","doi":"10.1016/j.crmicr.2024.100312","DOIUrl":"10.1016/j.crmicr.2024.100312","url":null,"abstract":"<div><div>Currently, the use of bio-inputs is increasing due to the need to reduce the use of agrochemicals. However, one of the limitations is to preserve the viability of the living microorganisms, so it is important to find an alternative that allows us to obtain different metabolites to produce it. We evaluated three different interactions (contact, diffusible and volatile compounds) <em>in vitro</em> in <em>Arabidopsis thaliana</em> (At) seedlings with the strain <em>Cytobacillus firmus</em> M10 and its filtered secondary metabolites (M10F). The results showed that the seedlings inoculated by contact with the filtrate (AtM10F) presented increases in root length (30 %) and leaf area (33 %), as well as in the volatile interaction (At/M10F) with respect to the uninoculated treatment. For both interactions, the seedlings inoculated with the bacteria by contact (AtM10) and volatile (At/M10) obtained greater biomass (48 and 57 %). Subsequently, an evaluation at the end of the <em>A. thaliana</em> cycle showed that the treatments obtained by contact and distance when reinoculated with the bacteria and the filtrate (AtM10, At-M10 and AtM10F) obtained 50 % more seed yield than the control treatment, while AtM10F presented 72 %, while At/M10F presented the highest no. of siliques and seeds, which increased the yield by 65 %. In the <em>Solanum lycopersicum</em> (Sl) experiment, the filtrate (SlM10F) showed significant differences in seedling height, leaf length and width (23, 24 and 36 %, respectively). It also promoted an increase in fresh and dry weight, producing a greater root area and larger leaves compared to the control (Sl) and the bacteria (SlM10). We performed a qualitative characterization of the secondary metabolites present in the filtrate, where we found 2,4-DTBP, sylvopinol, isophthaladehyde, and eicosane of interest with possible growth-promoting effects on <em>A. thaliana</em> and tomato. We identified volatile compounds present in plant-microorganism and plant-filtrate interactions as possible precursors in the induction of plant growth, among which phenols, alcohols, aldehydes, alkanes, and alkenes stand out. Most of the analyzed compounds have not been found in the literature with reports of growth promoters, is important to mention that due to their characteristic functional groups they can derive and trigger the synthesis of new molecules with agronomic application.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100312"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of universal stress protein Usp1413 in meropenem adaptive resistance and environmental stress responses in Acinetobacter baumannii","authors":"Sirui Zhang ,&nbsp;Jingdan Wang ,&nbsp;Rong Yu ,&nbsp;Haiping Liu ,&nbsp;Shuyan Liu ,&nbsp;Kai Luo ,&nbsp;Jin'e Lei ,&nbsp;Bei Han ,&nbsp;Yanjiong Chen ,&nbsp;Shaoshan Han ,&nbsp;E Yang ,&nbsp;Meng Xun ,&nbsp;Lei Han","doi":"10.1016/j.crmicr.2024.100332","DOIUrl":"10.1016/j.crmicr.2024.100332","url":null,"abstract":"<div><div>Although various mechanisms of carbapenem-resistance have been identified in the nosocomial pathogen <em>Acinetobacter baumannii</em>, the critical process of resistance evolution and the factors involved in are not well understood. Herein, we identified a universal stress protein Usp1413 which played an important role in adaptive resistance of <em>A. baumannii</em> to meropenem (MEM). Based on RNA-Seq and genome sequencing, Usp1413 was not only one of the most downregulated USPs, but also the bare one having mutation of tyrosine and glycine inserted at the site of 229-230 (YG229-230) under the stimulation of MEM. Deletion of Usp1413 resulted in increased MEM resistance. In addition, Usp1413 affected the bacterial abilities of biofilm formation and swarm motility, as well as helped <em>A. baumannii</em> response to various environmental stresses. These effects of Usp1413 were achieved by regulating its interaction proteins, within the functions of YigZ family protein, acetyltransferase, and SulP family inorganic anion transporter. The insertion mutation of YG229-230 influenced both the expression of interaction proteins and the phenotypes of bacteria. Finally, the promotor region of Usp1413 was convinced by point mutations. Overall, our findings identified the universal stress protein Usp1413 as a contributor involved in MEM adaptive resistance and responded to numerous environmental stresses. This study provides novel insights into the mechanism of universal stress proteins in participating antibiotic resistance, and affords a potential target for controlling drug resistance development in <em>A. baumannii</em>.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100332"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142932893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}