Current Research in Microbial Sciences最新文献

{"title":"Linalool exhibit antimicrobial ability against Elizabethkingia miricola by disrupting cellular and metabolic functions","authors":"Mingwang He ,&nbsp;Weiming Zhong ,&nbsp;Rongsi Dai ,&nbsp;Su Long ,&nbsp;Ying Zhou ,&nbsp;Tongping Zhang ,&nbsp;Boyang Zhou ,&nbsp;Tao Tang ,&nbsp;Linlin Yang ,&nbsp;Sifan Jiang ,&nbsp;Wenbin Xiao ,&nbsp;YanJiao Fu ,&nbsp;Jiajing Guo ,&nbsp;Zhipeng Gao","doi":"10.1016/j.crmicr.2025.100380","DOIUrl":"10.1016/j.crmicr.2025.100380","url":null,"abstract":"<div><div><em>Elizabethkingia miricola</em> is a gram-negative bacillus, a life-threatening pathogen in humans and animals. Linalool, a naturally occurring monoterpene alcohol found in plant volatile oils, exhibits highly effective antibacterial properties. This study investigated the antibacterial activity and mechanism of linalool against <em>E. miricola</em>. Initially, linalool showed potent antibacterial activity against <em>E. miricola</em>, with inhibition zone (ZOI), MIC, and MBC values of 36.41 ± 1.23 mm, 0.125 % (v/v, 1.0775 mg/mL), and 0.125 % (v/v, 1.0775 mg/mL), respectively. Secondly, it was observed by electron microscopy that linalool caused crumpling, depression, and size reduction of the cells. Linalool affected cell membrane integrity, causing membrane damage and rupture. Thirdly, transcriptome analysis suggested that linalool affected C5-branched-chain dicarboxylic acid metabolism and the biosynthesis of valine, leucine, and isoleucine, result in increased energy production to linalool stress. Linalool disrupted cell division and RNA function in <em>E. miricola</em>, and the cells responded to linalool-induced oxidative damage by up-regulating the expression of <em>msrB</em> and <em>katG</em> genes. Fourthly, metabolome analysis revealed an increase in metabolites related to the glycerophospholipid metabolic pathway and NADP content in <em>E. miricola</em>, which may be a metabolic response to linalool stress. Taken together, these findings provide a theoretical basis for the antibacterial mechanism of linalool and suggest potential applications for preventing <em>E. miricola</em> infections.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100380"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143705522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergence of Klebsiella pneumoniae ST14 co-harboring blaNDM-1, blaOXA-232, mcr-1.1, and a novel IncI1 tet(X4) plasmid, with evidence of ColKP3 mobilization under antibiotic pressure","authors":"Thanawat Phuadraksa,&nbsp;Yanisa Choominthong,&nbsp;Sineewanlaya Wichit,&nbsp;Sakda Yainoy","doi":"10.1016/j.crmicr.2025.100466","DOIUrl":"10.1016/j.crmicr.2025.100466","url":null,"abstract":"<div><div>Companion animals and environmental niches act as interconnected reservoirs of antimicrobial resistance (AMR) genes, facilitating their persistence and horizontal transfer across hosts and ecosystems. Pet-associated environments, within the One Health framework linking human, animal, and environmental health, remain an underrecognized source of AMR dissemination. Pet grooming facilities generate wastewater containing bacteria from animal skin, fecal matter, and contaminated surfaces, potentially acting as factors that facilitate environmental contamination and zoonotic transmission. Here, we describe the isolation and complete genomic characterization of an extensively drug-resistant <em>Klebsiella pneumoniae</em> strain from wastewater at a pet grooming facility in Bangkok, Thailand. Whole-genome sequencing identified the isolate as sequence type (ST) 14, a globally disseminated high-risk clone associated with multidrug resistance and clinical outbreaks. The strain harbored four clinically significant resistance genes, <em>bla</em>_NDM-1, <em>bla</em>_OXA-232, <em>mcr</em>-1.1, and <em>tet</em>(X4), each located on distinct plasmids. To our knowledge, this is the first report of <em>tet</em>(X4) in <em>K. pneumoniae</em> ST14. The gene was found on a novel IncI1-type plasmid with a composite transposon, suggesting recent acquisition through horizontal gene transfer. Conjugation assays confirmed high transfer efficiency and phenotypic tigecycline resistance. In this study, although <em>bla</em>_OXA-232 was carried on a non-conjugative ColKP3-type plasmid, colistin selection facilitated its transfer with plasmid size expansion, indicating antibiotic-driven mobilization. These findings highlight the evolutionary adaptability of <em>K. pneumoniae</em> ST14 and the risk posed by pet-associated wastewater as a reservoir for clinically important AMR genes. Integrated genomic surveillance and targeted One Health interventions are urgently needed to prevent environmental and zoonotic spread.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100466"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salmon nasal cartilage proteoglycan up-regulates Listeria monocytogenes-mediated immune response in mice","authors":"Akio Nakane ,&nbsp;Phawinee Subsomwong ,&nbsp;Tatsuji Takahashi ,&nbsp;Kenichi Ito ,&nbsp;Krisana Asano","doi":"10.1016/j.crmicr.2025.100465","DOIUrl":"10.1016/j.crmicr.2025.100465","url":null,"abstract":"<div><div>Proteoglycan (PG) is a complex glycohydrate that is widely distributed in the extracellular matrix. We have reported that daily oral administration of PG extracted from salmon (<em>Oncorhynchus keta</em>) nasal cartilage modulates the severity and proinflammatory cytokine responses in <em>Escherichia coli-</em>stimulated macrophages, and attenuates mouse models of various inflammatory diseases. Thus, PG has shown anti-inflammatory activities. In this study, we investigated the effect of salmon nasal cartilage PG on a bacterial infection by using a mouse model of infection with <em>Listeria monocytogenes</em>, which is a facultative intracellular pathogen. PG enhanced production of cytokines including TNF-α, IL-6, IL-12 and IL-10 by heat-killed <em>L. monocytogenes</em> in macrophages and dendritic cells. Daily oral administration of PG inhibited proliferation of <em>L. monocytogenes</em> in the livers of infected mice. IFN-γ and IL-12 production was enhanced in the PG-treated mice. Uptake of <em>L. monocytogenes</em> cells by PG-treated macrophages was decreased, followed reduction of intracellular bacteria growth. Differential proteomic analysis of mouse liver revealed that positive and negative immunological molecules for host defense against <em>L. monocytogenes</em> infection were modulated by PG treatment. These results demonstrated that PG can activate immune responses for host defense against <em>L. monocytogenes</em> infection through augmentation of cytokines and limiting infection of macrophages. The present study indicates that PG possesses quite different potentials, depending on the host conditions.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100465"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144931878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The irpT gene influences the multiple stresses tolerance by affecting cytochrome c in Lactococcus lactis N8","authors":"Dingyi Guo ,&nbsp;Chunhui Jia ,&nbsp;Xiaoting Xu ,&nbsp;Shan Jiang ,&nbsp;Wanjin Qiao ,&nbsp;Xiaxia He ,&nbsp;Per Erik Joakim Saris ,&nbsp;Haijin Xu ,&nbsp;Mingqiang Qiao","doi":"10.1016/j.crmicr.2025.100429","DOIUrl":"10.1016/j.crmicr.2025.100429","url":null,"abstract":"<div><div><em>Lactococcus lactis</em>, an internationally recognized safe species of Gram-positive bacteria, produces a variety of antimicrobial peptides. In this study, we examined the <em>irpT</em> gene of <em>L. lactis</em> N8 and its influence on the bacterial stress response. We used the Cre–loxP system to knock out the <em>irpT</em> gene from the <em>L. lactis</em> N8Δ<em>ply10300</em> genome. The mutant strains <em>L. lactis</em> N8Δ<em>ply10300</em> and <em>L. lactis N8</em>Δ<em>ply10300</em>Δ<em>irpT</em> showed higher nisin tolerance and nisin production than the wild-type strain <em>L. lactis</em> N8, and also obtained tolerance for multiple stressors. The biological characteristics of the <em>irpT</em> gene and its encoded protein in <em>L. lactis</em> N8 were investigated. Deletion of the <em>irpT</em> gene significantly reduced the content of cytochrome c, enhanced the tolerance of the strain to various environmental stresses, resulted in increased antimicrobial peptide nisin production and improved nisin resistance. Deletion of <em>irpT</em> gene conferred resistance to multiple stressors, including lysozyme, lactic acid, and antibiotics, and demonstrated resistance against multiple bacteriostatic mechanisms. Our findings lay the foundation for further research into the physiological processes of stress resistance in lactic acid bacteria.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100429"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144534317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A T cell-based ubiquitin-mediated mRNA vaccine provides cross-protection against H1N1 and B influenza viruses in mice","authors":"Yaxin Di ,&nbsp;Ziliang Wang ,&nbsp;Zilin Ren ,&nbsp;Haixin Huang ,&nbsp;Songhui Yang ,&nbsp;Chenchao Zhang ,&nbsp;Shibo Liang ,&nbsp;Pengyuan Dong ,&nbsp;Wanbo Tai ,&nbsp;Xinyu Zhuang ,&nbsp;Mingyao Tian","doi":"10.1016/j.crmicr.2025.100457","DOIUrl":"10.1016/j.crmicr.2025.100457","url":null,"abstract":"<div><div>Given the persistent antigenic drift of seasonal influenza viruses and the continuous threat of emerging pandemics, there is an urgent necessity to develop novel influenza vaccines capable of conferring broad-spectrum immunity against multiple viral subtypes. CD8⁺ T cells provide a promising approach to achieving such protection because of their ability to recognize conserved internal antigens. Particularly, the highly cross-reactive internal nucleoprotein of influenza virus demonstrates remarkable efficacy in safeguarding against infection caused by diverse strains. Ubiquitination modification is critical for the differentiation and functionality of CD8⁺ T cells, thereby modulating the immune response. In this study, three mRNA vaccines were designed using the influenza virus nucleoprotein as an immunogen, including wild-type N protein (WT-N), ubiquitinated wild N protein (Ub-WT-N), and ubiquitinated rearrangement N protein (Ub-Re-N). After immunizing C57BL/6 mice, both WT-N and Ub-WT-N vaccines elicited antibody production, while the Ub-Re-N group exhibited enhanced cellular immune response without inducing antibody production. Subsequently challenged with influenza viruses, the vaccinated mice showed significant protection against mortality and weight loss caused by H1N1 and influenza B strains. Notably, depletion of CD8⁺ T cells led to a substantial reduction in the protective efficacy of the Ub-Re-N vaccine. In conclusion, the mRNA vaccine encoding Ub-Re-N confers potent defense against influenza virus infection through induction of a robust antigen-specific T cell response.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100457"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144841433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sampling frequency matters: mapping of the healthy infants' gut microbiome during the first year of life","authors":"Lenka Micenková ,&nbsp;Soňa Smetanová ,&nbsp;Jacek Marciniak ,&nbsp;Kristýna Brodíková ,&nbsp;Dominika Polaštík Kleknerová ,&nbsp;Barbora Lakotová ,&nbsp;Barbora Zwinsová ,&nbsp;Vojtěch Thon ,&nbsp;Petra Vídeňská ,&nbsp;Eva Budinská","doi":"10.1016/j.crmicr.2025.100470","DOIUrl":"10.1016/j.crmicr.2025.100470","url":null,"abstract":"<div><div>Early life events significantly influence the developing gut microbiome, yet the response time and duration of microbiome changes to specific factors, such as vaccination or solid food introduction, remain unclear. Consequently, determining the optimal sampling frequency to monitor gut microbiome development is challenging. This study monitored gut microbiome plasticity using 16S rRNA gene sequencing almost daily in one infant (A) and weekly in 12 others (B–M) during their first year. Changes were linked to external factors and their duration analyzed. Three bacterial colonization groups emerged: \"Early-life colonizers,\" \"Re-appearing colonizers,\" and \"Later-colonizers.\" Weekly sampling underestimated microbiome variability, as individual samples within the same week differed by over 1 Shannon index, and most of the weekly coefficients of variation of different alpha diversity indices in the first 23 weeks were higher than 10 %. Alpha diversity variability decreased with age, but beta diversity variability remained high. Key events like solid food introduction and probiotics caused gradual but significant bacterial composition changes, with effects varying among infants. Sparse weekly sampling hindered a detailed understanding of the impact of maternal microbiome, diet, probiotics, vaccinations, and unforeseen variables. Analysis of weekly variability in alpha and beta diversity suggests that such rare sampling may not be sufficient in terms of the outcomes of interest.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100470"},"PeriodicalIF":5.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145060194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of compost as a soil amendment on the structure and function of fungal diversity in saline–alkali soil","authors":"Daolong Xu ,&nbsp;Xiaowen Yu ,&nbsp;Yusen Zhang ,&nbsp;Yaru Liu ,&nbsp;Chunmei Chen ,&nbsp;Li Li ,&nbsp;Shuming Fan ,&nbsp;Xingrong Lu ,&nbsp;Xiujuan Zhang","doi":"10.1016/j.crmicr.2025.100405","DOIUrl":"10.1016/j.crmicr.2025.100405","url":null,"abstract":"<div><div>Agricultural productivity is significantly impeded by salinization, which also adversely affects the environment. The use of compost as a soil conditioner has been shown to effectively ameliorate saline-alkali soils. Nonetheless, there is a paucity of research investigating the effects of compost on microbial populations within these soils. This study utilized high-throughput sequencing technology to specifically investigate the impact of compost addition on fungal diversity and the composting process in saline-alkali soils. The aim was to elucidate the effects of compost incorporation on the composition, structure, and functional group characteristics of fungal communities in such soils. The findings indicate that fungal diversity varied across different fermentation stages, with the predominant functional microbiota differing at each stage. Ascomycota, Mortierellomycota, and Basidiomycota were identified as the dominant fungal taxa in the soil. FUNGuild analysis predicted that the functional capacity of the rhizosphere soil fungal community was significantly enhanced by saprotrophic fungi in saline-alkali soil, resulting in improved soil quality. The Redundancy Analysis indicated that the main environmental factors affecting the fungal community were soil pH, organic matter, and soil enzymes. The findings from this research could offer a theoretical foundation for enhancing saline-alkali soils with microbial biofertilizers and boosting agricultural productivity.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"9 ","pages":"Article 100405"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144203424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canine-derived Weissella confusa ZJUIDS-D034 and Enterococcus faecalis ZJUIDS-D016 combat aging by regulating gut microbiota","authors":"Nan Chen ,&nbsp;Zexu Yu ,&nbsp;Xuan Ji ,&nbsp;Siyi Zhang ,&nbsp;Chongwei Yu ,&nbsp;Teresa G. Valencak ,&nbsp;Fushan Shi ,&nbsp;Daxi Ren","doi":"10.1016/j.crmicr.2025.100381","DOIUrl":"10.1016/j.crmicr.2025.100381","url":null,"abstract":"<div><div>Old age raises the susceptibility of age-related disease in domestic dogs. Discovering effective anti-aging interventions is key for mitigating age-related disease and conserving “healthspan” in pet dogs. In this study, 2 bacterial strains were isolated from canine feces. After screening and identifying the strains, <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 were chosen to intervene during <em><span>d</span>-</em>galactose-induced senescence in mice. We found that administering <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 improved the aging phenotype of mice, including an increase in antioxidant activity, a decrease in pro-inflammatory cytokines, and the restoration of intestinal and liver tissue damage. In addition, <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 lead to changes in the structure of intestinal microbiota in aging mice. Specifically, there was a decrease in the abundance of the <em>Cyanobacteria</em> and an increase in the abundance of <em>Akkermansia</em> and <em>Lactobacillus</em>. More importantly, there was a significant increase in acetic acid, a short-chain fatty acid, due to intervention with the 2 strains. This increase might be attributed to higher <em>Akkermansia</em>. We show that the modulation of gut microbiota and metabolism in aging mice may be a promising strategy through which <em>Weissella confusa</em> ZJUIDS-D034 and <em>Enterococcus faecalis</em> ZJUIDS-D016 might exert their anti-aging effects.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100381"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Irrigation water and soil chemistry shape fungal guilds in date palm soils, enhancing pathotroph abundance under saline groundwater irrigation","authors":"Subha Chandran ,&nbsp;Dinesh Sanka Loganathachetti ,&nbsp;Balamurugan Sadaiappan ,&nbsp;Sanjay Swarup ,&nbsp;Sunil Mundra","doi":"10.1016/j.crmicr.2025.100370","DOIUrl":"10.1016/j.crmicr.2025.100370","url":null,"abstract":"<div><div>Saline groundwater irrigation is a predominant practice, especially in date palm (<em>Phoenix dactylifera L</em>.) farms in arid agroecosystems with scarce freshwater resources. Despite its economic importance, the influence of saline groundwater irrigation on bulk soil fungi remains overlooked. This study examined how saline groundwater irrigation affects fungal diversity, community structure, and assembly processes. Bulk soils from date palm farms with distinct irrigation sources (freshwater and saline groundwater), were analysed, and fungal community analyses were done using the internal transcribed spacer 2 (ITS2) sequencing. Soils irrigated with saline groundwater had a lower percentage (27%) of unique operational taxonomic units (OTUs) compared to freshwater (33.3%). Fungal richness negatively correlated with soil pH. Differences observed in overall and guild-specific fungal communities, with irrigation water electrical conductivity (EC) emerging as a pivotal factor distinguishing between the two irrigation sources. Notably, pathotrophs abundance was significant in soils irrigated with saline groundwater. Furthermore, the dominant pathotroph <em>Fusarium</em>, exhibited drift-based assembly process and was observed to be high under saline groundwater irrigation. Our study reveals that groundwater salinity reduces the number of unique OTUs and alters fungal communities at the overall and guild levels. This insight aids agricultural improvement in regions where saline groundwater is a predominant water source.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100370"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expansion of maltose/sucrose related transporters in Ascomycetes and their association with corresponding disaccharide utilization","authors":"Li Xu ,&nbsp;Alessia Manassero ,&nbsp;Berend Snel ,&nbsp;Ronald P. de Vries ,&nbsp;Mao Peng","doi":"10.1016/j.crmicr.2025.100368","DOIUrl":"10.1016/j.crmicr.2025.100368","url":null,"abstract":"<div><div>Sugar transporters (STs) play a crucial role in mediating sugar uptake in fungi and have been increasingly studied due to their important biological roles and industrial potential. In this study, we performed a comprehensive phylogenetic analysis of STs across the fungal kingdom, including species from Ascomycota, Basidiomycota, Mucoromycota and Zoopagomycota. The results revealed a striking diversity of STs among these fungal phyla with respect to their genomic content and predicted sugar specificity. Particularly, we identified a remarkable expansion of maltose/sucrose STs and a strong co-expansion of intracellular α-1,4-glucosidases and invertases in Ascomycota compared to other fungal phyla. In addition, growth profiles support that the utilization of maltose and sucrose across a diverse set of fungi is roughly determined by the presence of both corresponding STs and functionally related hydrolases. This study enhances our understanding of evolutional diversity of fungal STs and provides new insights into metabolic engineering of fungi towards more efficient conversion of plant-derived sugars for relevant industrial applications.</div></div>","PeriodicalId":34305,"journal":{"name":"Current Research in Microbial Sciences","volume":"8 ","pages":"Article 100368"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}